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1.
J Nanosci Nanotechnol ; 20(3): 1495-1503, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492312

RESUMO

A new enzymatic biosensor worn on eyeglasses has been developed for low-noise and noninvasive determination of lactate in human sweat during physical exercise. The Os (osmium)-complex, the electron mediator between the enzyme and the electrode, was first immobilized on a flexibly printed carbon electrode. Then, a gel membrane with the stereoscopic reticular structure of lactate oxidase and horseradish peroxidase was casted on the electrode to form the biosensor. Linearity of the biosensor was observed for up to 25 mM lactate in a phosphate buffered solution of pH 7.0. Chemical selectivity was evaluated by adding common interferent species such as ascorbic acid, glucose and uric acid to the lactate. The negligible current interference indicated excellent discriminatory selectivity of the biosensor. Applied to an analysis of the real sweat lactate dynamics of healthy subjects during cycling exercise, the amperometric profiles of the biosensors reflected changes in sweat lactate that depended on physical exercise intensity. Compared with other reported epidermal biosensors attached to the arm or leg, our biosensor not only exhibited a similar current change tendency but also rarely suffered from deformational interference due to their forehead measurement position. Such a successful application of real-time monitoring of sweat lactate means that eyeglass-bound biosensors hold considerable promise in the physical exercise and biomedical fields.

2.
J Nanosci Nanotechnol ; 20(3): 1740-1748, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492338

RESUMO

Pure CuC2O4·xH2O and CuC2O4·xH2O/carbon nanotubes (CNTs) composites are synthesized by a low-temperature hydrothermal process. The structure and morphology of the products are analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TG) and Raman spectrum. The results demonstrate that the as-prepared CuC2O4·xH2O takes on a microsphere-like morphology, all CuC2O4·xH2O/CNTs nanocomposites are constructed by the intertwining of tabular CuC2O4·xH2O nanoparticles (NPs) and CNTs to form a tanglesome net. When evaluated as an anode materials for lithium ion batteries (LIBs), all CuC2O4·xH2O/CNTs electrodes possess higher reversible discharge capacities (more than 1000 mAh g-1) than the pure CuC2O4·xH2O, up to 200th cycle at a current density of 100 mA g-1. The results illustrate that the addition of CNTs can enhance the electrochemical performance of CuC2O4·xH2O. Overall, CuC2O4·xH2O/CNTs composite can be a promising candidate used as a promising anode for LIBs.

3.
Dev Comp Immunol ; 102: 103486, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31473265

RESUMO

The complement system is a crucial component of the innate immune system that links innate and adaptive immunity. CL-11, a protein similar to Mannose-binding lectin (MBL), plays significant role in the innate immune system in mammals and fish, serving as an initiator of the lectin pathway of complement activation. In this study, a CL-11 homolog (TfCol-11) was identified in roughskin sculpin (Trachidermus fasciatus), and its expression and role in immune responses were characterized. The open reading frame of TfCol-11 is 795 bp long, encoding a 264 amino acid polypeptide. The deduced amino acid sequence of this protein is highly homologous to sequences in other teleosts, and is similar to vertebrate CL-11, containing a canonical collagen-like region, a carbohydrate recognition domain, and a neck region. Recombinant TfCol-11 purified from Escherichia coli(E.coli) was able to bind to different microbes in a Ca2+-independent manner. Meanwhile, a 993 bp-long of partial MASP cDNA with a 96 bp 5' untranslated region (UTR) was also cloned from roughskin sculpin, containing 299 amino acids and consisting of three domains (CUB-EGF-CUB). qRT-PCR indicated that TfCol-11 and MASP mRNAs were predominately co-expressed in the liver. The temporal expression of TfCol-11 and MASP were both drastically up-regulated in the liver, skin, and blood by LPS challenge. Recombinant TfCol-11 purified from E.coli BL21(DE3) was able to agglutinate some bacteria in a Ca2+-dependent manner. In addition, an in vitro pull-down experiment demonstrated that TfCol-11 was able to bind to MASP, and in vivo experiments showed that TfCol-11 was associated with increased membrane attack complex (MAC) levels. It is therefore possible that TfCol-11 may plays a role in activating the complement system and in the defense against invading microorganisms in roughskin sculpin.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31567715

RESUMO

BACKGROUND AND AIM: Open surgery remains the major approach to treat hepatocellular carcinoma, and laparoscopy-assisted liver resection has been recommended as a superior treatment. However, the efficacy of laparoscopic surgery versus open surgery for cirrhotic patients is under debate. Therefore, the aim of this meta-analysis was to compare the clinical outcomes of laparoscopic and open resection of hepatocellular carcinoma in patients with cirrhosis. METHODS: Electronic databases were searched for eligible literature updated on November 2018. After rigorous review of quality, the data were extracted from eligible trials. All the data were pooled with the corresponding 95% confidence interval using RevMan software. Sensitivity analyses and heterogeneity were quantitatively evaluated. RESULTS: Fourteen trials met the inclusion criteria. According to the pooled result of surgery duration, laparoscopic surgery was associated with significantly shorter hospital stay [STD mean difference (SMD) = -0.61, 95% confidence interval -0.89 to -0.32; P < 0.0001], lower intraoperative blood loss (SMD = -0.56, 95% confidence interval -0.99 to -0.12; P = 0.01), fewer complications (odds ratio = 0.38, 95% confidence interval 0.28 to 0.52; P < 0.00001) and lower transfusion rate (odds ratio = 0.58, 95% confidence interval 0.36-0.93; P = 0.02). Nevertheless, there was no remarkable difference in operative time (SMD = 0.17, 95% confidence interval -0.25 to -0.59; P = 0.42) between the two groups. The pooled analysis of overall survival showed that laparoscopic surgery did not achieve benefit compared with open surgery (P = 0.02). Moreover, the pooled results of three subgroups indicated that laparoscopic surgery was associated with significantly better disease-free survival (P < 0.05). CONCLUSION: The current analysis indicates that laparoscopic liver resection for hepatocellular carcinoma improved intraoperative and disease-free survival, with similar overall survival compared to the open procedure. Laparoscopic surgery may serve as a safe and feasible alternative for selected hepatocellular carcinoma patients with cirrhosis.

5.
Adv Mater ; : e1902807, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566828

RESUMO

1D nanomaterials with high aspect ratio, i.e., nanowires and nanotubes, have inspired considerable research interest thanks to the fact that exotic physical and chemical properties emerge as their diameters approach or fall into certain length scales, such as the wavelength of light, the mean free path of phonons, the exciton Bohr radius, the critical size of magnetic domains, and the exciton diffusion length. On the basis of their components, aspect ratio, and properties, there may be imperceptible connections among hundreds of nanowires prepared by different strategies. Inspired by the heredity system in life, a new concept termed the "nanowire genome" is introduced here to clarify the relationships between hundreds of nanowires reported previously. As such, this approach will not only improve the tools incorporating the prior nanowires but also help to precisely synthesize new nanowires and even assist in the prediction on the properties of nanowires. Although the road from start-ups to maturity is long and fraught with challenges, the genetical syntheses of more than 200 kinds of nanostructures stemming from three mother nanowires (Te, Ag, and Cu) are summarized here to demonstrate the nanowire genome as a versatile toolbox. A summary and outlook on future challenges in this field are also presented.

6.
J Agric Food Chem ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31578056

RESUMO

Astaxanthin is a carotenoid of high commercial value because of its excellent antioxidative, anti-inflammatory and anticancer properties. Here, we developed a novel strategy for improving the production of astaxanthin via morphology and oxidative stress engineering. First, we identified the morphology-/membrane-and oxidative stress-related genes, which should be knocked down, using the CRISPRi system. Deleting the morphology-/membrane-related genes (lpp and bamB) and the oxidative stress-related genes (uspE and yggE) generated longer and larger cells with higher ROS levels, thus enhancing the production of astaxanthin and decreasing cell growth. To not only improve cell growth but also obtain longer and larger cells with higher ROS levels, a complementary expression system using a temperature-sensitive plasmid was established. Complementarily expressing the morphology-/membrane-related genes (lpp and bamB) and the oxidative stress-related genes (uspE and yggE) further improved the production of astaxanthin to 11.92 mg/g dry cell weight (DCW) in shake flask cultures.

7.
Reprod Fertil Dev ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31578166

RESUMO

Mitochondrial DNA (mtDNA) has been widely associated with complex traits in farm animals. The present study evaluated the effects of mtDNA on litter size in pigs. Mitogenome sequencing of 1017 sows distinguished 232 variations, including 229 single nucleotide polymorphisms and three indels, which constituted 11 haplotypes and further clustered into two haplogroups that differed significantly (P<0.05) in litter size. In order to explain the associations between the effect of haplogroup on litter size and different maternal origins, extant mitogenome sequences were used for phylogenetic or principal component analyses. The results of these analyses led to the identification of two groups, representing Chinese and European origins. The haplotypes corresponding to high litter size were all in the Chinese cluster, whereas haplotypes corresponding to low litter size were all in the European cluster. The results of this study suggest that the effect of haplogroup on litter size in the pig could be caused by diverse maternal origins, and that mtDNA haplogroup may be a marker for genetic selection for pig litter size.

8.
Sci Rep ; 9(1): 14355, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31586104

RESUMO

Two new species of Lasiodiplodia (Lasiodiplodia endophytica and Lasiodiplodia magnoliae) are described and illustrated from Magnolia forests in Yunnan, China. Endophytic and saprobic Lasiodiplodia pseudotheobromae and endophytic L. thailandica are new records from this host. The internal transcribed spacers (ITS), part of the translation elongation factor-1α (tef1) and partial ß-tubulin (tub2) sequence data were analyzed to investigate the phylogenetic relationships of the new species with other Lasiodiplodia species. Lasiodiplodia magnoliae is phylogenetically sister to L. mahajangana and L. pandanicola but morphologically distinct from L. mahajangana in having larger conidia. Lasiodiplodia endophytica is most closely related to L. iraniensis and L. thailandica and the three species can be distinguished from one another by 2 base pair differences in ITS and three or four base pair differences in tef1. The new collections suggest that Magnolia forest plants are good hosts for Lasiodiplodia species with endophytic and saprobic life-styles.

9.
J Environ Qual ; 48(4): 792-802, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31589688

RESUMO

Cold agricultural regions are important sites of global food production. This has contributed to widespread water quality degradation influenced by processes and hydrologic pathways that differ from warm region analogues. In cold regions, snowmelt is often a dominant period of nutrient loss. Freeze-thaw processes contribute to nutrient mobilization. Frozen ground can limit infiltration and interaction with soils, and minimal nutrient uptake during the nongrowing season may govern nutrient export from agricultural catchments. This paper reviews agronomic, biogeochemical, and hydrological characteristics of cold agricultural regions and synthesizes findings of 23 studies that are published in this special section, which provide new insights into nutrient cycling and hydrochemical processes, model developments, and the efficacy of different potentially beneficial management practices (BMPs) across varied cold regions. Growing evidence suggests the need to redefine optimum soil phosphorus levels and input regimes in cold regions to allow achievement of water quality targets while still supporting strong agricultural productivity. Practices should be considered through a regional and site-specific lens, due to potential interactions between climate, hydrology, vegetation, and soils, which influence the efficacy of nutrient, crop, water, and riparian buffer management. This leads to differing suitability of BMPs across varied cold agricultural regions. We propose a systematic approach (""), to achieve water quality objectives in variable and changing climates, which combines nutrient transport process onceptualization, nderstanding BMP functions, redicting effects of variability and change, onsideration of producer input and agronomic and environmental tradeoffs, practice daptation, nowledge mobilization, and valuation of water quality improvement.

10.
J Environ Qual ; 48(4): 869-879, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31589691

RESUMO

Reducing eutrophication in surface water is a major environmental challenge in many countries around the world. In cold Canadian prairie agricultural regions, part of the eutrophication challenge arises during spring snowmelt when a significant portion of the total annual nutrient export occurs, and plant residues can act as a nutrient source instead of a sink. Although the total mass of nutrients released from various crop residues has been studied before, little research has been conducted to capture fine-timescale temporal dynamics of nutrient leaching from plant residues, and the processes have not been represented in water quality models. In this study, we measured the dynamics of P and N release from a cold-hardy perennial plant species, alfalfa ( L.), to meltwater after freeze-thaw through a controlled snowmelt experiment. Various winter conditions were simulated by exposing alfalfa residues to different numbers of freeze-thaw cycles (FTCs) of uniform magnitude prior to snowmelt. The monitored P and N dynamics showed that most nutrients were released during the initial stages of snowmelt (first 5 h) and that the magnitude of nutrient release was affected by the number of FTCs. A threshold of five FTCs was identified for a greater nutrient release, with plant residue contributing between 0.29 (NO) and 9 (PO) times more nutrients than snow. The monitored temporal dynamics of nutrient release were used to develop the first process-based predictive model controlled by three potentially measurable parameters that can be integrated into catchment water quality models to improve nutrient transport simulations during snowmelt.

11.
J Environ Qual ; 48(4): 803-812, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31589694

RESUMO

Managing P export from agricultural land is critical to address freshwater eutrophication. However, soil P management, and options to draw down soil P have received little attention in snowmelt-dominated regions because of limited interaction between soil and snowmelt. Here, we assessed the impacts of soil P drawdown (reducing fertilizer P inputs combined with harvest removal) on soil Olsen P dynamics, runoff P concentrations, and crop yields from 1997 to 2014 in paired fields in Manitoba, Canada. We observed that Olsen P concentrations in the 0- to 5-cm soil layer were negatively correlated with the cumulative P depletion and declined rapidly at the onset of the drawdown practice (3.1 to 5.4 mg kg yr during 2007-2010). In both snowmelt runoff and rainfall runoff, concentrations of total dissolved P (TDP) were positively correlated with the concentrations of soil Olsen P. Soil P drawdown to low to moderate fertility levels significantly decreased mean annual flow-weighted TDP concentrations in snowmelt runoff from 0.60 to 0.30 mg L in the field with high initial soil P and from 1.17 to 0.42 mg L in the field with very high initial soil P. Declines in TDP concentration in rainfall runoff were greater. Critically, yields of wheat ( spp.) and canola ( L.) were not affected by soil P depletion. In conclusion, we demonstrate that relatively rapid reductions in P loads are achievable at the field scale via managing P inputs and soil P pools, highlighting a management opportunity that can maintain food security while improving water security in cold regions.

12.
J Environ Qual ; 48(4): 850-868, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31589697

RESUMO

The use of cover crops and crop residues is a common strategy to mitigate sediment and nutrient losses from land to water. In cold climates, elevated dissolved P losses can occur associated with freeze-thaw of plant materials. Here, we review the impacts of cover crops and crop residues on dissolved P and total P loss in cold climates across ∼41 studies, exploring linkages between water-extractable P (WEP) in plant materials and P loss in surface runoff and subsurface drainage. Water-extractable P concentrations are influenced by plant type and freezing regimes. For example, WEP was greater in brassica cover crops than in non-brassicas, and increased with repeated freeze-thaw cycles. However, total P losses in surface runoff and subsurface drainage from cropped fields under cold climates were much lower than plant WEP, owing to retention of 45 to >99% of released P by soil. In cold climatic regions, cover crops and crop residues generally prevented soil erosion and loss of particle-bound P during nongrowing seasons in erodible landscapes but tended to elevate dissolved P loss in nonerodible soils. Their impact on total P loss was inconsistent across studies and complicated by soil, climate, and management factors. More research is needed to understand interactions between these factors and plant type that influence P loss, and to improve the assessment of crop contributions to P loss in field settings in cold climates. Further, tradeoffs between P loss and the control of sediment loss and N leaching by plants should be acknowledged.

13.
Mol Pharm ; 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31589818

RESUMO

Colorectal cancer (CRC) is one of the most common cancers worldwide. MicroRNAs (miRNAs) play a vital role in a variety of biology processes. Our previous work identified miR-139-5p as a tumor suppressor gene overexpressed in CRC that assisted in inhibiting progression of cancer. The main challenge of miRNAs as therapeutic agents is their rapid degradation in plasma, poor uptake and off-target effects. Therefore, the development of miRNA-based therapies is necessary. In this study, we developed a cationic liposome-based nanoparticles loaded with miR-139-5p (miR-139-5p-HSPC/DOTAP/Chol/DSPE-PEG2000-COOH nanoparticles, MNPs) and surface-decorated with epithelial cell adhesion molecule (EpCAM) aptamer (Apt) (miR-139-5p-EpCAM Apt-HSPC/DOTAP/Chol/DSPE-PEG2000-COOH nanoparticles, MANPs) for the targeted treatment of CRC. The size of MANPs was 150.3±8.8 nm, which had a round-shaped appearance and functional dispersion capabilities. It also showed negligible hemolysis in the blood. MANPs markedly inhibited the proliferation, migration and invasion of one or more CRC cell lines in-vitro. Furthermore, we demonstrated the uptake and targeting ability of MANPs in-vivo and in-vitro. MANPs inhibits the growth of HCT8 cells in-vitro, and has a significant tumor suppressive effect on subcutaneous HCT8 colorectal tumor mice. Our results demonstrated that MANPs were effective carrier approach to deliver therapeutic miRNAs to CRC.

14.
Respir Res ; 20(1): 161, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31590675

RESUMO

BACKGROUND: There is growing literature suggesting a link between vitamin D and asthma lung function, but the results from systematic reviews are conflicting. We conducted this meta-analysis to investigate the relation between serum vitamin D and lung function in asthma patients. METHODS: Major databases, including OVID, MEDLINE, Web of Science and PUBMED, were searched until 10th October 2018. All published observational studies related to vitamin D and asthma were extracted. All meta-analyses were performed using Review Manager 5.3.5. RESULTS: This quantitative synthesis found that asthma patients with low vitamin D levels had lower forced expiratory volume In 1 s (FEV1) (mean difference (MD) = - 0.1, 95% CI = - 0.11 to - 0.08,p < 0.01;I2 = 49%, p = 0.12) and FEV1% (MD = - 10.02, 95% CI = - 11 to - 9.04, p < 0.01; I2 = 0%, p = 0.82) than those with sufficient vitamin D levels. A positive relation was found between vitamin D and FEV1 (r = 0.12, 95% CI = 0.04 to 0.2, p = 0.003; I2 = 59%,p = 0.01), FEV1% (r = 0.19, 95% CI = 0.13 to 0.26, p < 0.001; I2 = 42%, p = 0.11), forced vital capacity (FVC) (r = 0.17, 95% CI = 0.00 to 0.34, p = 0.05; I2 = 60%, p = 0.04), FEV1/FVC (r = 0.4, 95% CI = 0.3 to 0.51, p < 0.001; I2 = 48%, p = 0.07), and the asthma control test (ACT) (r = 0.33, 95% CI = 0.2 to 0.47, p < 0.001; I2 = 0%, p = 0.7). Subgroup analysis indicated that the positive correlation between vitamin D and lung function remained significant in both children and adults. CONCLUSIONS: Our meta-analysis suggested that serum vitamin D levels may be positively correlated with lung function in asthma patients. Future comprehensive studies are required to confirm these relations and to elucidate potential mechanisms.

15.
Zhongguo Zhong Yao Za Zhi ; 44(14): 2896-2901, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602831

RESUMO

In 2013,China pharmaceutical regulatory department issued guiduance on postmarketing drug safety monitoring for industry. It aimed to encourage industry to carry out postmarketing drug safety monitoring including hospital-based intensive monitoring of postmarketing Chinese patent medicine. Subsequently,more and more such kind of studies have been performed all over China. However,in view of the current situation in this field,the development of hospital-based intensive monitoring of postmarketing Chinese patent medicine lacks standardization,such as unreasonable design,omission of reports about adverse drug reactions,inadequate process of quality control,non-standardized interpretation of adverse reactions,etc. Therefore,it is necessary to formulate relevant technical specifications to guide this area. The developing of this technical specification refered to the international post-marketing safety monitoring model and advanced design concepts and methods. We developed it under the guidance of relevant laws,regulations and technical documents in China. Meanwhile the characteristics of Chinese patent medicines and the real situation in this area were considered. The aim of this technical specification is to obtain the incidence,type,degree and clinical manifestation of adverse drug reactions of Chinese patent medicines,to find new risk signals of adverse reactions,to identify risk factors,and to provide a basis for the formulation of risk management and control plans. This specification has been approved by China association of Chinese medicine which is numbered T/CACM011-2016.

16.
Zhongguo Zhong Yao Za Zhi ; 44(14): 2926-2931, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602834

RESUMO

Diemailing~® Kudiezi Injection( DKI) is widely used in the treatment of cerebral infarction,coronary heart disease and angina pectoris. Long-term clinical application and related research evidence showed that DKI has a good effect in improving the clinical symptoms of cardiovascular and cerebrovascular diseases. However,this injection has not been included in any clinical practice guideline. It has been found that the use of DKI is in wrong way in clinical practice in recent years. Therefore,clinical experts from the field of cardiovascular and cerebrovascular diseases nationwide are invited to compile this expert consensus in order to guide clinicians.GRADE system is used to grade the quality of evidence according to different outcomes according to degrading factors. Then it forms the recommendation or consensus suggestion through the nominal group method. The formation of expert consensus mainly considers six factors: quality of evidence,economy,efficacy,adverse reactions,patient acceptability and others. Based on these six aspects,if the evidence is sufficient,a " recommendation" supported by evidence is formed,and GRADE grid voting rule is adopted. If the evidence is insufficient,a " consensus suggestions" will be formed,using the majority voting rule. In this consensus,the clinical indications,efficacy,safety evidences and related preliminary data of DKI were systematically and comprehensively summarized in a concise and clear format,which could provide valuable reference for the clinical use of DKI. This consensus has been approved by China association of Chinese medicine which is numbered GS/CACM 202-2019.

17.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3520-3525, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602917

RESUMO

The effect of triptolide( TP) on VEGFA,SDF-1,CXCR4 pathway were investigated in vitro to explore the mechanism in improving platelet activation in patients with ankylosing spondylitis( AS). Peripheral blood mononuclear cells( PBMC) were used for the experiment and divided into 4 groups: normal group( NC),model group( MC),triptolide group( TP),and AMD3100 group. The optimal concentration of TP was measured by the MTT method. The expressions of TNF-α,IL-1ß,IL-4,IL-10,VEGFA and VEGFR were detected by ELISA. The expressions of SDF-1,CXCR4 and VEGFA were detected by real-time quantitative PCR( RT-qPCR).The expressions of SDF-1,CXCR4,VEGFA and VEGFR were detected by Western blot. The expression levels of CD62 p,CD40 L and PDGFA were detected by immunofluorescence. MTT results showed that medium-dose TP had the strongest inhibitory effect on cells at24 h. The results of ELISA and PCR showed that TP inhibited mRNA expressions of IL-1ß,TNF-α,VEGFA,VEGFR and SDF-1,CXCR4 and VEGFA. The results of Western blot indicated that TP inhibited SDF-1,CXCR4 and VEGFA,VEGFR protein expressions; immunofluorescence results indicate that TP can inhibit the expressions of CD62 p,CD40 L,PDGFA. TP may regulate platelet activation by down-regulating SDF-1,CXCR4,VEGFA and VEGFR mRNA expressions,thereby down-regulating IL-1ß and TNF-αexpressions,and up-regulating the expressions of IL-4 and IL-10 cytokines.

18.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3526-3532, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602918

RESUMO

This paper aims to investigate the effect of oral administration of Tripterygium Glycosides Tablets combined with traditional Chinese medicine on immune inflammatory index in patients with rheumatoid arthritis,in order to explore the compatibility mode of traditional Chinese medicine in the treatment of rheumatoid arthritis. Medical records of hospitalized patients with rheumatology at the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine from June 2012 to December 2017 were collected. The combined administration of Tripterygium Glycosides Tablets and traditional Chinese medicine was adopted for the experimental group,while the simply administration of Tripterygium Glycosides Tablets were adopted for the control group. SPSS 21. 0 was used to analyze the changes of general conditions and immune inflammatory metabolic indexes in the two groups of RA patients. The association rules were analyzed by SPSS Clementine 14. 2 software Apriori module,and the random walk model was evaluated by ORACLE 10 g tool. The results showed that a total of 1 220 patients with rheumatoid arthritis met the requirements of this study,including 322 in the experimental group and 898 in the control group. Before treatment,there was no significant difference in age and duration between the two groups. The difference value of Ig A,Ig G,RF,CCP-AB,hs-CRP and ESR in the two groups of RA patients decreased before and after treatment,and the experimental group was superior to the control group in reduction of Ig A,Ig G,RF,CCP-AB,hs-CRP and ESR.The control group was superior to the experimental group in reduction of Ig M( P<0. 01 or P<0. 05). Compared with before treatment,ALT,AST,ALP,GGT,CREA,BUN,b-MG,MA,TRU and Ig U all increased,with statistically significant differences( P<0. 01).The UA of the two groups of RA patients decreased after treatment,with statistically significant differences( P<0. 01). The experimental group was superior to the control group in reduction of UA,with statistically significant differences( P < 0. 05 or P < 0. 01). The herbs adopted in the prescriptions of 1 220 patients were mainly classified into four categories,namely spleen-sweating herbs,blood-activating and stasis-relieving herbs,phlegm and phlegm-relieving herbs,and heat-clearing and antidote herbs. The results of association rule analysis indicated a significant correlation between the single-flavored Tripterygium Glycosides Tablets,oral Chinese medicine and immune inflammation,and improvement of liver and kidney function indexes. The results of the random walk model analysis indicated that the experimental group's Ig M and hs-CRP were superior to those of the control group in terms of random fluctuation maximum,walking positive growth rate,comprehensive evaluation index increasing rate,comprehensive improvement rate,comprehensive evaluation index recording times,and expected improvement value. The results of this study showed that the single administration of Tripterygium Glycosides Tablets can effectively improve the immune inflammatory metabolic index of patients with rheumatoid arthritis,and the combined administration of Tripterygium Glycosides Tablets and traditional Chinese medicine could alleviate the immune inflammatory index of RA patients and reduce liver and kidney dysfunction compared with simple oral administration. The comprehensive evaluation Ig M and hs-CRP in the group of combined administration of Tripterygium Glycosides Tablets and traditional Chinese medicine were better than those in the group of the Tripterygium Glycosides Tablets. There was a long-term correlation between the comprehensive evaluation index and the intervention measures of the two groups of patients.

19.
Technol Health Care ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31594271

RESUMO

BACKGROUND: Few studies have investigated interleukin (IL)-33 as a potential proinflammtory cytokine in the joint cavity following subchondral fracture occurring during osteonecrosis of the femoral head (ONFH). OBJECTIVE: The aim of the present study was to investigate whether IL-33 and other proinflammtory cytokines, in addition to cartilage degeneration, were elevated in the joint cavity following subchondral fracture. METHODS: IL-33 levels were analyzed in the plasma, synovial fluid, and bone marrow aspirate, and the levels of IL-6, IL-17, IL-1ß, tumor necrosis factor (TNF)-α, matrix metallopeptidase (MMP)-13, and carboxy-terminal telopeptides of type II collagen (CTX-II) in synovial fluid were also quantitated to compare the levels of these proteins between stages II and III. Macro- and microscopic articular cartilage were evaluated and compared between stages II and III in the same patient. Moreover, immunohistochemical staining of MMP-13 and Col-II were examined. RESULTS: Of the 33 patients enrolled in the present study, 15 had hips in stage II and 18 had hips in stage III. IL-33 levels in all three sample types were significantly higher in stage III. Synovial fluid IL-6 and CTX-II levels were significantly higher in stage III. A clear step-off region in the weight-bearing area was macroscopically observed in the stage III femoral head. Microscopic evaluation showed symmetrical cartilage matrix deposition around the chondrocytes in the stage II articular cartilage, unlike that in stage III. Immunohistochemical staining of the stage III articular cartilage samples revealed positive MMP-13 staining in chondrocytes and decreased Col-II deposition in the matrix. CONCLUSIONS: The present study revealed elevated IL-33 and IL-6 levels and cartilage degeneration in stage III ONFH.

20.
J Thromb Haemost ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31573759

RESUMO

BACKGROUND: Heparins are usually produced from animal tissues. It is now possible to synthesize heparins. This provides the perspectives to overcome shortages of heparin, to optimize biological effects, and to reduce adverse drug effects. Heparins interact with platelet factor 4 (PF4), which can induce an immune response causing thrombocytopenia. This side effect is called heparin-induced thrombocytopenia (HIT). We characterized the interaction of PF4 and HIT antibodies with oligosaccharides of 6-, 8-, 10-, and 12-mer size and a hyper-sulfated 12-mer (S12-mer). METHODS: We utilized multiple methodologies including isothermal calorimetry, circular dichroism spectroscopy, single molecule force spectroscopy (SMFS), enzyme immunosorbent assay (EIA), and platelet aggregation test to characterize the interaction of synthetic heparin analogs with PF4 and anti-PF4/heparin antibodies. RESULTS: The synthetic heparin-like compounds display stronger binding characteristics to PF4 than animal-derived heparins of corresponding lengths. Upon complexation with PF4, 6-mer and S12-mer heparins showed much lower enthalpy, induced less conformational changes in PF4, and interacted with weaker forces than 8-, 10-, and 12-mer heparins. Anti-PF4/heparin antibodies bind weaker to complexes formed between PF4 and heparins ≤8-mer than with complexes formed between PF4 and heparins ≥10-mer. Addition of one sulfate group to the 12-mer resulted in a S12-mer which showed substantial changes in its binding characteristics to PF4. CONCLUSIONS: We provide a template for characterizing interactions of newly developed heparin-based anticoagulant drugs with proteins, especially PF4 and the resulting potential antigenicity.

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