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1.
J Proteomics ; 210: 103541, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31614210

RESUMO

Recently it was discovered that thioproline, an unnatural analog of proline, can arise in vivo from the reaction of cysteine and formaldehyde in cells under oxidative stress. Sequence-specific bioincorporation of thioproline into proteins was studied via shotgun proteomics of Escherichia coli (E. coli) cells. In a strain auxotrophic for proline, thioproline was found widely incorporated in lieu of proline when the cells were incubated with thioproline. In total 1428 proteins and 235 distinct thioproline-containing peptides were identified. Label-free relative quantitation revealed 102 differentially expressed proteins (82 up-regulated and 20 down-regulated) in the thioproline-treated group (with thioproline in the medium) relative to the control group (with proline in the culture medium). Pathway enrichment analysis of the differentially expressed proteins showed that amino acid biosynthesis and protein synthesis has been most affected by thioproline exposure, as expected. Phenotypically, the thioproline-treated group was found to exhibit slower cell growth and stronger antioxidant capacity relative to the control. SIGNIFICANCE: Thioproline is a secondary metabolite of formaldehyde and a structural analog of proline. It is also known to exhibit a wide variety of pharmaceutical properties, but its exact biochemical role in the cell has not been elucidated. In this paper, we studied thioproline misincorporation (in lieu of proline) events during protein synthesis in E. coli. Global proteome profiling revealed that thioproline is extensively misincorporated throughout the proteome in E. coli cells exposed to thioproline, and pathways related to amino acid and protein biosynthesis are up-regulated. In addition, we demonstrated that pretreatment with thioproline appeared to increase E. coli cells' capacity to tolerate oxidative stress. Our findings suggest a novel explanation of thioproline's known antioxidative properties. This is, to our knowledge, the first ever study of thioproline misincorporation at the proteome level in any organism.

2.
Arch Iran Med ; 22(10): 612-626, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31679364

RESUMO

BACKGROUND: Acute and severe infections are an absolute indication for the use of intravenous broad-spectrum antibiotics. However, previous studies have found inconsistent clinical advantages of prolonged (extended [≥3-hour infusion] or continuous [24-hour fixed rate infusion]) over intermittent (6, or 8, or 12 interval hours infusion) infusion. The clinical superiority between prolonged and intermittent infusion in treating acute and severe infections thus continues to be elusive. We conducted a meta-analysis to summarize all published randomized controlled trials (RCTs), prospective and retrospective observational studies to determine whether prolonged infusion, compared to intermittent infusion, is correlated with lower mortality and better clinical outcome. METHODS: We performed a literature search using MEDLINE (source PubMed, January 1, 1966 to August 31, 2018) and EMBASE (January 1, 1980 to August 31, 2018) with no restrictions to collect RCTs and observational studies comparing prolonged infusion with intermittent infusion of the same intravenous administered antibiotics among adult hospitalized patients. A total of 43 studies including 30 RCTs, 5 prospective observational studies and 8 retrospective observational studies were identified. RESULTS: In comparison with intermittent infusion, prolonged infusion of antibiotics was associated with a reduction in all-cause mortality (pooled relative risk [RR] = 0.77, 95% confidence interval [CI] = 0.66-0.89) and improvement in clinical cure (RR = 1.11, 95% CI = 1.04-1.19), which was also observed in subgroups such as non-RCTs (mortality, RR = 0.63, 95% CI = 0.48-0.81; clinical cure RR = 1.33, 95% CI = 1.13-1.57) or studies with patients and APACHE II scores 15 (mortality, RR = 0.74, 95% CI 0.63-0.89; clinical cure RR = 1.19, 95% CI = 1.07-1.32). Moreover, in RCTs, mortality (RR = 0.86, 95% CI 0.72-1.03) between the two dosing strategies was not remarkably changed but clinical cure (RR = 1.07, 95% CI = 1.01-1.13) showed a significant advantage for prolonged infusion. Additionally, no significant differences in mortality between the two dosing strategies was found (RR = 0.87, 95% CI = 0.70-1.09) but a distinct improvement in clinical cure was observed (RR = 1.14, 95% CI = 1.02-1.28) in the prolonged infusion group for septic patients. Among two infusion modes, statistically significant severe adverse events were not reported (RR=0.83, 95% CI = 0.62-1.13). CONCLUSION: Better outcomes in hospitalized patients, especially in those who were critical ill, were reported in prolonged infusion of intravenous antibiotics compared with traditional intermittent infusion.

3.
Cancer Res ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690669

RESUMO

The emerging role of heparanase in tumor initiation, growth, metastasis, and chemoresistance is well recognized, encouraging the development of heparanase inhibitors as anticancer drugs. Unlike the function of heparanase in cancer cells, little attention has been given to heparanase contributed by cells composing the tumor microenvironment. Here, we focused on the cross-talk between macrophages, chemotherapy, and heparanase and the combined effect on tumor progression. Macrophages were markedly activated by chemotherapeutics paclitaxel (PCT) and cisplatin, evidenced by increased expression of pro-inflammatory cytokines, supporting recent studies indicating that chemotherapy may promote rather than suppress tumor re-growth and spread. Strikingly, cytokine induction by chemotherapy was not observed in macrophages isolated from heparanase-knockout mice, suggesting macrophage activation by chemotherapy is heparanase-dependent. PCT-treated macrophages enhanced the growth of lewis lung carcinoma tumors which was attenuated by a CXCR2 inhibitor. Mechanistically, PCT and cisplatin activated methylation of histone H3 on lysine 4 (H3K4) in wild-type but not heparanase-knockout macrophages. Furthermore, the H3K4 presenter WDR5 functioned as a molecular determinant that mediated cytokine induction by PCT. This epigenetic, heparanase-dependent host-response mechanism adds a new perspective to the tumor-promoting functions of chemotherapy, and offers new treatment modalities to optimize chemotherapeutics.

4.
Mass Spectrom Rev ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31682024

RESUMO

Metabolism is the collection of biochemical reactions enabled by chemically diverse metabolites, which facilitate different physiological processes to exchange substances and synthesize energy in diverse living organisms. Metabolomics has emerged as a cutting-edge method to qualify and quantify the metabolites in different biological matrixes, and it has the extraordinary capacity to interrogate the biological significance that underlies metabolic modification and modulation. Liquid chromatography combined with mass spectrometry (LC/MS), as a robust platform for metabolomics analysis, has increased in popularity over the past 10 years due to its excellent sensitivity, throughput, and versatility. However, metabolomics investigation currently provides us with only phenotype data without revealing the biochemical functions and associated mechanisms. This limitation indeed weakens the core value of metabolomics data in a broad spectrum of the life sciences. In recent years, the scientific community has actively explored the functional features of metabolomics and translated this cutting-edge approach to be used to solve key multifaceted questions, such as disease pathogenesis, the therapeutic discovery of drugs, nutritional issues, agricultural problems, environmental toxicology, and microbial evolution. Here, we are the first to briefly review the history and applicable progression of LC/MS-based metabolomics, with an emphasis on the applications of metabolic phenotyping. Furthermore, we specifically highlight the next era of LC/MS-based metabolomics to target functional metabolomes, through which we can answer phenotype-related questions to elucidate biochemical functions and associated mechanisms implicated in dysregulated metabolism. Finally, we propose many strategies to enhance the research capacity of functional metabolomics by enabling the combination of contemporary omics technologies and cutting-edge biochemical techniques. The main purpose of this review is to improve the understanding of LC/MS-based metabolomics, extending beyond the conventional metabolic phenotype toward biochemical functions and associated mechanisms, to enhance research capability and to enlarge the applicable scope of functional metabolomics in small-molecule metabolism in different living organisms.

5.
Fitoterapia ; : 104416, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31704261

RESUMO

Vitiligo is a common depigmentary disease characterized as diagnosis simplicity and cure difficulty in view of the ambiguity of etiology, thus novel and effective treatments are urgently needed. Paeoniflorin, the major active compound extracted from the root of Paeonia lactiflora Pall, a traditional Chinese medicine, has been validated pharmacological properties such as antioxidant stress, a theory participating in the occurrence of vitiligo, but the effect on melanogenesis is still unclear. In this study, melanosythesis effect of paeoniflorin and the potential mechanism were evaluated. We found that treatment with paeoniflorin at the concentration of 10 µg/ml significantly increased melanin content and intracellular tyrosinase activity of human melanocytes, in accordance with the elevation of protein levels of microphthalmia-associated transcription factor (MITF), tyrosinase-related protein 1 (TRP-1). In addition, we also investigated that paeoniflorin promoted phosphorylation of cAMP-response element binding (CREB) and extracellular signal-regulated kinase (ERK) without affecting p38 and c-Jun N-terminal kinase (JNK). These results demonstrated that paeoniflorin had a synergistic effect on normal human melanocytes via ERK/CREB pathway with up-regulation of MITF and TRP-1, enhancing melanin synthesis. Meanwhile, the milder pathological changes in vitiligo mice treat with paeoniflorin also confirmed its potential in treating vitiligo. To sum up, we suggest that paeoniflorin may be a potential medicine of vitiligo treatment in clinical.

6.
Cancer Lett ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31705927

RESUMO

Triple-negative breast cancer (TNBC) is characterized by high vascularity, but anti-angiogenic therapies show poor efficacy. Centromere protein U (CENPU), a centromere component essential for mitosis, is associated with tumorigenesis in multiple cancers; however, little is known of its role in breast cancer. Here, we investigate its expression and function of promoting angiogenesis in TNBC. Immunohistochemical staining revealed high CENPU expression in TNBC tissue and high CENPU levels correlated significantly with poor distant metastasis-free and overall survival. Knockdown of CENPU in TNBC cells inhibited vascular endothelial growth factor A (VEGFA) production and significantly reduced tube formation and migration of human umbilical vein endothelial cells in vitro. In a mouse xenograft model, CENPU knockdown reduced TNBC tumor growth concomitant with a reduction in CD31+ microvessel density. Mechanistic studies revealed that CENPU promoted angiogenesis by inhibiting the ubiquitination and proteasomal degradation of cyclooxygenase-2 (COX-2), leading to increased activation of the COX-2-p-ERK-HIF-1α-VEGFA signaling pathway. Taken together, our results demonstrate a critical role for CENPU in COX-2-mediated signaling for angiogenesis, and identify a role of CENPU in regulating angiogenesis in TNBC.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31696201

RESUMO

As the most common cancer and one of the leading causes of cancer-associated mortality, breast cancer continues to need more key molecules to regulate its progression. F-box and leucine-rich repeat protein 19 antisense RNA 1 (known as FBXL19-AS1) is a long non-coding RNA (lncRNA) which has been reported as an oncogene in several types of human cancers. However, the specific downstream targets of FBXL19-AS1 remain unknown. In this study, we set out to find more reliable downstream molecules of FBXL19-AS1 in breast cancer. FBXL19-AS1 was expressed at a high level in breast cancer cells. Loss-of-function experiments revealed that silencing FBXL19-AS1 could impair cell proliferation and induce cell apoptosis in breast cancer. In addition, the location of FBXL19-AS1 in the cytoplasm was detected by fluorescent in situ hybridization assay, while FBXL19-AS1 regulated the expression of Forkhead box M1 (FOXM1) by directly absorbing miR-876-5p. Through rescue assays, it was observed that FOXM1 overexpression recovered the inhibited tumor growth caused by FBXL19-AS1 downregulation. We affirmed the function of FBXL19-AS1 in breast cancer and described the mechanism of the FBXL19-AS1/miR-876-5p/FOXM1 axis. The current work presents the molecular mechanism which underlies FBXL19-AS1 in breast cancer and suggests a comprehensive, feasible FBXL19-AS1-mediated therapeutic approach for treating breast cancer.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31654599

RESUMO

Palladium-catalyzed alkene-directed cross-coupling of aryl iodide with another aryl halide through C-H arylation opens a unique avenue for unsymmetrical biaryl-derived molecules. However, homo-coupling of aryl iodides often eroded the overall synthetic efficiency. Reported herein is a highly chemoselective Pd(0)-catalyzed alkyne-directed cross-coupling of aryl iodides with bromophenols, which was subsequently followed by phenol dearomatization to furnish a very attractive [2+2+1] spiroannulation. Noteworthy, possible homo-coupling of aryl iodides was not observed at all. Mechanistic studies indicated that five-membered aryl/vinyl palladacycle was most likely accounted for promoting the key step of biaryl cross-coupling.

9.
BMJ Open ; 9(10): e031812, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31594897

RESUMO

OBJECTIVES: Soluble urokinase plasminogen activated receptor (suPAR) is a biomarker that may predict the occurrence of focal segmental glomerulosclerosis (FSGS); however, there is still controversy about whether suPAR can predict FSGS. In this study, we performed a systematic evaluation and meta-analysis to prove whether suPAR can predict FSGS, and to detect a threshold concentration of suPAR that can be used to diagnose FSGS. In addition, a threshold concentration of suPAR for the diagnosis of FSGS was proposed. DESIGN: Systematic review and meta-analysis. DATA SOURCES: We systematically searched PubMed, Embase, Cochrane Library, Web of Science and China Biology Medicine databases for studies published from the inception dates to 1 December 2018. ELIGIBILITY CRITERIA: (1) Data involving the suPAR level were from blood samples; (2) FSGS was diagnosed by biopsy; and (3) randomised controlled trials, cohort studies, case-control studies and cross-sectional studies. DATA EXTRACTION AND SYNTHESIS: Initially, a total of 364 studies were searched, among which 29 studies were finally included. In addition, seven studies described the cut-off value of suPAR, which ranged from 2992.6 to 5500 pg/mL. RESULTS: The results showed that the suPAR levels in the primary FSGS group were significantly higher when compared with that in the normal control group (p<0.001; standard mean difference (SMD): 2.56; 95% CI 1.85 to 3.28), and significant differences were observed in the secondary FSGS and in the normal control group (p<0.001; SMD: 1.68; 95% CI 1.37 to 1.98). A suPAR concentration of 3000 pg/mL may be the best threshold for the diagnosis of primary FSGS (sensitivity=0.72; specificity=0.88; area under the curve=0.85). CONCLUSION: Our results suggested that suPAR might be a potential biomarker for predicting primary and secondary FSGS. In addition, our data showed that a suPAR concentration of 3000 pg/mL might be used as a threshold for the diagnosis of FSGS. TRIAL REGISTRATION NUMBER: CRD42019120948.

10.
Comput Intell Neurosci ; 2019: 5367217, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31662739

RESUMO

This paper presents a fault detection and diagnosis (FDD) method, which uses one-dimensional convolutional neural network (1-D CNN) and WaveCluster clustering analysis to detect and diagnose sensor faults in the supply air temperature (T sup) control loop of the air handling unit. In this approach, 1-D CNN is employed to extract man-guided features from raw data, and the extracted features are analyzed by WaveCluster clustering. The suspicious sensor faults are indicated and categorized by denoting clusters. Moreover, the T c acquittal procedure is introduced to further improve the accuracy of FDD. In validation, false alarm ratio and missing diagnosis ratio are mainly used to demonstrate the efficiency of the proposed FDD method. Results show that the abrupt sensor faults in T sup control loop can be efficiently detected and diagnosed, and the proposed method is equipped with good robustness within the noise range of 6 dBm∼13 dBm.

11.
Chem Commun (Camb) ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31660558

RESUMO

In this communication, the mechanism of how surface chirality affects amyloid-ß (Aß) fibrillation was firstly unravelled at the molecular level: a chiral surface serves to control the 2D-diffusion and surface residence time of Aß molecules via the chiral recognition with Aß to allow precursor Aß to laterally diffuse and collide with each other for oligomerization and fibrillation. Surface chirality that shortens the surface residence time of Aß, for example, R-cysteine modification with carboxylic, secondary amine and thiol groups surrounding the chiral center, can retard Aß oligomerization and fibrillation. This work is essential to a deeper fundamental understanding of the effects of surface chirality on amyloidosis processes as well as the development of chiral materials to inhibit Aß fibrillation.

12.
ACS Appl Mater Interfaces ; 11(43): 40585-40591, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31589394

RESUMO

Developing new filters for the effective removal of high-temperature particulate matter is of great importance but still remains a challenge. Herein, we demonstrate a novel and facile strategy for producing hierarchical ceramic foams with three-dimensional interconnected porous architecture via the combination of chemical grafting of pore-forming agent and polyurethane foaming technique. Carbamate groups are directly grafted onto carbon black surface to enhance its dispersion. Abundant micrometer-sized pores are generated on the cell walls of porous frameworks to form three-dimensional interconnected porous architectures, resulting in the mullite foam with high particulate matter removal efficiency and relatively low pressure drop. The optimized mullite foam exhibits integrated properties of high particulate matter removal efficiency (96.7%), ultralow pressure drop (35 Pa), and outstanding recyclability. Our results open new opportunities for fabricating efficient particulate matter filters used in high-temperature environmental fields.

13.
J Diabetes Res ; 2019: 9696521, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31565658

RESUMO

Interventional therapies, such as percutaneous transluminal angioplasty and endovascular stent implantation, are used widely for the treatment of diabetic peripheral vascular complications. Reendothelialization is an essential process in vascular injury following interventional therapy, and hyperglycemia in diabetes mellitus (DM) plays an important role in damaging endothelial layer integrity, leading to the retardance of reendothelialization and excessive neointimal formation. Notoginsenoside Fc (Fc), a novel saponin isolated from Panax notoginseng, effectively counteracts platelet aggregation. Nevertheless, the potential effects and molecular mechanisms of Fc on reendothelialization have yet to be explored. In this study, we present novel findings that show the benefit of Fc in accelerating reendothelialization and alleviating excessive neointimal formation following carotid artery injury in diabetic Sprague-Dawley rats in vivo. Simultaneously, the decreased autophagy of the injured carotid artery in diabetic rats was restored by Fc treatment. Our in vitro results also demonstrated that Fc promoted endothelial cell proliferation and migration under high-glucose treatment by increasing autophagy. In summary, this study supported the notion that Fc could accelerate reendothelialization following vascular injury in diabetic rats by promoting autophagy, suggesting that Fc may exert therapeutic benefits for early endothelial injury and restenosis following intervention in diabetes-associated vascular diseases.

14.
Medicine (Baltimore) ; 98(38): e17034, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31567940

RESUMO

BACKGROUND: Pericardial infection caused by Acinetobacter baumannii is rare, particularly that of carbapenem-resistant A baumannii (CRAB). CASE PRESENTATION: We describe a rare case of purulent pericarditis due to CRAB in a 76-year-old man with acute myocardial infarction and acute kidney injury. The man was admitted to the intensive care unit for a catheter-related bloodstream infection. Pericardial effusion was detected via the bedside X-ray and ultrasound, and pericardiocentesis was performed. Cultures of the pericardial fluid, catheter tip, and blood independently revealed the presence of CRAB. These findings confirmed a diagnosis of purulent pericarditis. CONCLUSIONS: Clinicians should be reminded that CRAB infection can lead to purulent pericarditis, particularly in patients with congestive heart failure or renal insufficiency.


Assuntos
Infecções por Acinetobacter/diagnóstico , Acinetobacter baumannii/isolamento & purificação , Lesão Renal Aguda/diagnóstico , Infarto do Miocárdio sem Supradesnível do Segmento ST/diagnóstico , Pericardite/diagnóstico , Infecções por Acinetobacter/complicações , Acinetobacter baumannii/efeitos dos fármacos , Lesão Renal Aguda/complicações , Idoso , Carbapenêmicos/farmacologia , Diagnóstico Diferencial , Farmacorresistência Bacteriana , Evolução Fatal , Humanos , Masculino , Infarto do Miocárdio sem Supradesnível do Segmento ST/complicações , Pericardiocentese , Pericardite/complicações , Pericardite/diagnóstico por imagem , Tomografia Computadorizada por Raios X
15.
Sci Adv ; 5(10): eaax6525, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31633028

RESUMO

Functionally related genes tend to be chromosomally clustered in eukaryotic genomes even after the exclusion of tandem duplicates, but the biological significance of this widespread phenomenon is unclear. We propose that stochastic expression fluctuations of neighboring genes resulting from chromatin dynamics are more or less synchronized such that their expression ratio is more stable than that for unlinked genes. Consequently, chromosomal clustering could be advantageous when the expression ratio of the clustered genes needs to stay constant, for example, because of the accumulation of toxic compounds when this ratio is altered. Evidence from manipulative experiments on the yeast GAL cluster, comprising three chromosomally adjacent genes encoding enzymes catalyzing consecutive reactions in galactose catabolism, unequivocally supports this hypothesis and elucidates how disorder in one biological phenomenon-gene expression noise-could prompt the emergence of order in another-genome organization.

16.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3221-3225, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602875

RESUMO

The non-starch polysaccharides,mainly composed of glucomannans,are the major bioactive compounds in Dendrobium catenatum. In order to evaluate the quality of the medicinal materials and guide the production and processing,a quantification method of non-starch polysaccharides was established by stems of D. catenatum C15 strain collected from the pear epiphytic cultivation. The non-starch polysaccharides were obtained by " water extraction,α-amylase pretreatment,and alcohol precipitation once" method. The contents of starches,non-starch polysaccharides and monosaccharides were analyzed. In addition,the system suitability was tested. Compared with method of the Chinese Pharmacopoeia( 2015 edition),the contents of total polysaccharides,glucose,and mannose were decreased by 20. 9%,58. 8% and 1. 6% respectively. The method effectively digested starch and retained non-starch polysaccharides,and the analysis result was accurate and repeatable. Therefore,it is suitable for the content measurement of non-starch polysaccharides of D. catenatum. Furthermore,it could be an alternative method for quality control of D. catenatum and a reference in the determination of non-starch polysaccharides in other starch-containing medicinal materials.


Assuntos
Dendrobium/química , Polissacarídeos/análise , Monossacarídeos/análise , Compostos Fitoquímicos/análise , Amido/análise
17.
ACS Nano ; 13(10): 11603-11612, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31518116

RESUMO

Nanofibrous aerogels constructed solely by ceramic components with temperature-invariant hyperelasticity could have broad technological implications in extreme environments. However, creating such materials has proven to be extremely challenging. Despite the results from laboratory, those aerogels are, unfortunately, still plagued with issues that would retard their further application: inferior structural integrity, failure at large compressive deformation, high production cost, and inability to withstand rigorous working conditions. To tackle these challenges, we report a facile strategy combining the chemical vapor deposition process and layer-by-layer self-assembly to construct hyperelastic SiC nanofibrous aerogels with three-dimensional porous architecture and improved structural integrity. The resultant aerogels outperform their natural counterparts and most state-of-the-art ceramic nanofibrous aerogels in their capability to quickly recover from large compressive deformation (50% strain), function in a wide range of temperatures, from -196 °C to 1100 °C in air, maintain high particle matter removal efficiency of >99.96%, and rapidly absorb various organic solvents and oils with high capacity and robust recoverability. Nanofibrous aerogels constructed by such a versatile method could provide fresh insights into the exploration of multifunctional nanofibrous aerogels for a variety of applications in extreme environments.

18.
Shock ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31490358

RESUMO

BACKGROUND: Soluble urokinase-type plasminogen activator receptor (suPAR) has the potential to diagnose infectious diseases. Due to the lack of reliable biomarkers and the importance of timely diagnosis for sepsis treatment, we conducted this systematic review and meta-analysis to evaluate the value of suPAR diagnosis and prognosis for sepsis. METHODS: PubMed, Embase, Web of Science and Cochrane Library databases were searched for studies, which reported the value of suPAR diagnosis and/or prognosis in patients with sepsis. RESULTS: 30 studies involving 6906 patients were included. Sensitivity and specificity of suPAR for diagnosing sepsis were 0.76 [95% confidence interval (CI): 0.63-0.86] and 0.78 (95%CI: 0.72-0.83), respectively. The area under the summary receiver operating characteristic curve (AUC) was 0.83 (95%CI: 0.80-0.86). Pooled sensitivity and specificity for predicting mortality were 0.74 (95%CI: 0.67-0.80) and 0.70 (95%CI: 0.63-0.76), respectively, with AUC of 0.78 (95%CI: 0.74-0.82). In addition, AUC for differentiating sepsis from systemic inflammatory response syndrome (SIRS) was 0.81 (95%CI: 0.77-0.84), and the sensitivity and specificity were 0.67 (95%CI: 0.58-0.76) and 0.82 (95%CI: 0.73-0.88) respectively. CONCLUSION: suPAR is a feasible biomarker for timely diagnosis and prognosis of sepsis. Compared to effective value of procalcitonin (PCT) identified by previous meta-analysis, suPAR has similar clinical guiding value, while suPAR exhibits higher specificity, which can facilitate the deficiencies of PCT. suPAR also shows a diagnostic value in differentiating sepsis from SIRS. Considering the lack of biomarkers for sepsis and the similar clinical value of suPAR and PCT, suPAR should be considered as a biomarker in clinical practice for sepsis.

19.
Can J Diabetes ; 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31515158

RESUMO

Extracorporeal shock wave therapy (ESWT) as a new adjuvant therapy has shown a potential capability to promote diabetic foot ulcer (DFU) healing. The purpose of this study was to assess the efficacy and safety of ESWT on the healing of DFUs. The Cochrane Library, PubMed, Embase, Web of Science, China Biology Medicine and reference lists were searched for studies published up to December 2018. Randomized controlled trials of any design, including ESWT for patients with DFU, were included. Two reviewers extracted data, including the wound surface area (WSA), percentage of re-epithelialization, population of complete cure and unchanged and other related outcomes. Eight randomized controlled trials (N=339) were included. ESWT was found to be associated with a greater reduction of WSA by 1.54 cm2, and increase of re-epithelialization by 26.31%. A greater population with complete cure was found at the end of treatment (risk ratio [RR] = 2.22; 95% confidence interval [CI], 1.46 to 3.40); however, there was no statistically significant difference at the end of follow up (p=0.052). It can also reduce treatment inefficiency by 4.8-fold (95% CI, 0.12 to 0.37). In addition, ESWT also showed a higher superiority than hyperbaric oxygen therapy in the population for complete cure and unchanged ulcer (RR=1.83; 95% CI, 1.14 to 2.94 and RR=0.25; 95% CI, 0.13 to 0.48, respectively). ESWT is a feasible adjuvant treatment for DFUs. It can effectively improve the complete cure rate, shorten the healing period of DFUs and significantly reduce treatment ineffectiveness. This can provide new therapeutic ideas for clinical practice of intractable and recurrent DFUs.

20.
Int Immunopharmacol ; 76: 105840, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31487614

RESUMO

The purpose of this research was to evaluate the therapeutic effects of gentiopicroside (GPS) on adjuvant-induced arthritis (AA) rats. Rats were injected with complete Freund's adjuvant (CFA) for 0.1 mL in the right hind paw to induce AA. Thirty rats from three groups were treated with GPS (30, 60, 90 mg/kg) from day 15 to day 26. Arthritis was evaluated by arthritis index, paw volume, paw thickness, and X-ray. The effect of GPS on inflammation was assessed by measuring the levels of interleukin (IL)-1ß, tumor necrosis factor (TNF)-α, IL-6 and IL-17, as well as related mRNA. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), glutathione (GSH) protein carbonyl (PCO) and malondialdehyde (MDA) were measured to assess the effect of GPS on oxidative stress. These results indicate that GPS increases the levels of GSH-Px, SOD and GSH, and reduces the levels of MDA and PCO. GPS can significantly down-regulate the levels of IL-1ß, TNF-α, IL-6 and IL-17, as well as related mRNA. In addition, X-ray and histopathological results show that GPS has a therapeutic effect on joints in AA rats. In summary, the therapeutic effects of GPS on AA rats are associated with anti-inflammation and antioxidation.

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