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1.
Biogerontology ; 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32026209

RESUMO

The cochlear basilar membrane (CBM) contains inner hair cells and outer hair cells that convert sound waves into electrical signals and transmit them to the central auditory system. Cochlear aging, the primary reason of age-related hearing loss, can reduce the signal transmission capacity. There is no ideal in vitro aging model of the CBM. In this study, we cultured the CBM, which was dissected from the cochlea of the C57BL/6 mice 5 days after birth, in a medium containing 20 mg/mL, 40 mg/mL, or 60 mg/mL D-galactose (D-gal). Compared with the control group, the levels of senescence-associated ß-galactosidase were increased in a concentration-dependent manner in the CBM of the D-gal groups. In addition, levels of the mitochondrial superoxide and patterns of an age-related mitochondrial DNA3860-bp deletion were significantly increased. The ATP levels and the membrane potential of the mitochondrial were significantly decreased in the CBM of the D-gal groups compared with the control group. Furthermore, in comparison with the control group, damaged hair cell stereocilia and a loss of inner hair cell ribbon synapses were observed in the CBM of the D-gal groups. A loss of hair cells and activation of caspase-3-mediated outer hair cell apoptosis were also observed in the CBM of the high-dose D-gal group. These insults induced by D-gal in the CBM in vitro were similar to the ones that occur in cochlear natural aging in vivo. Thus, we believe that this is a successful in vitro aging model using cultured CBM. These results demonstrate the effects of mitochondrial oxidative damage on presbycusis and provide a reliable aging model to study the mechanisms of presbycusis in vitro.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32031775

RESUMO

Colorimetric chemosensors have attracted tremendous interest for sensing hazardous substances in an uncomplicated and economical manner. Herein, a series of push-pull dicyanovinyl-substituted oligothiophene derivatives were designed and the impacts of different end-cappers on their photophysical properties were comprehensively investigated. Interestingly, combined with a zinc porphyrin derivative (Zn-TPP), one dicyanovinyl-substituted oligothiophene derivative (NA-3T-CN) can be further developed into colorimetric and fluorescent sensor array for dual-mode detection of aliphatic amines and hydrazine. The obtained sensors showed satisfactory results between optical response and analyte's concentration both in selective single-sensor type and in enhanced multisensory mode. Base on the fluorescence change of the NA-3T-CN system, the detection limit for N2H4 was calculated to be around 1.22 × 10-5 mol/L in THF. The stained TLC-supported sensor array offers obvious optical changes for down to 0.5wt% hydrazine solution for naked-eye sensing. Aromatic amine like aniline has no obvious effect on the dicyanovinyl-substituted oligothiophene derivatives. We also found that a zinc porphyrin derivative has an obvious colorimetric response to the presence of hydrazine, ethanolamine, and aniline. Furthermore, smartphone-enabled readout system and data treatment based on RGB changes of the sensor array were performed and the discrimination capability among hydrazine, aliphatic amines and aromatic amine was satisfactory. In this regard, related push-pull oligothiophene derivatives not only can be regarded as models for a fundamental understanding of the relationship between molecular structure and photophysical properties, but also present potential applications in the field of real-time and visual detecting of hazardous chemicals.

3.
Neuroscience ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32032574

RESUMO

Cochlear ribbon synapses formed between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) are immature at birth and they require dramatic morphological and functional developments to achieve auditory maturation in postnatal mice. However, the mechanism underlying this remodeling process of cochlear ribbon synapse remains elusive. Here, we report that autophagy is necessary for the development and maturation of cochlear ribbon synapses in mice. In this study, significantly high levels of LC3B (a widespread marker of autophagy) were found in the cochlea from postnatal day 1 (P1) to P15, which then decreased at P28 to P30. Treatment of mice at P7 with rapamycin or 3-methyladenine (activator and inhibitor of autophagy, respectively) for 7 days led to the significant elevations of hearing threshold across frequencies from P15 to P30. Moreover, abnormal morphology of cochlear ribbon synapses and reduced IHC exocytosis function were detected from P15 to P30, which were likely associated to hearing impairment. Thus, our study demonstrated that autophagy was required for remodeling of cochlear ribbon synapses and provided a new insight into autophagy-related hearing disorder during auditory development. Furthermore, we implicated a novel therapeutic target for sensorineural hearing loss.

4.
Poult Sci ; 99(2): 1225-1231, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32036971

RESUMO

This study was undertaken to assess the effect of various levels of intramuscular fat (IMF: <0.5%, 0.5-0.99%, 1.0-1.49%, 1.5-1.99%, 2.0-2.49%, and >2.5%) on the physical meat quality of partridge. Physical characteristics such as moisture, pH, shear force, water-holding capacity (WHC), and color, along with IMF, were measured on 414 partridges (pectoral muscle). In this study, partridge meat was described as a kind of tender and moderately juicy meat with a nice color. Additionally, tenderness was significantly different between IMF < 0.5% and IMF > 0.5% levels (P < 0.01). IMF >0.5% also obtained higher value of WHC than IMF <0.5% although the difference was statistically nonsignificant. IMF levels did not affect pH or color. The results showed that IMF content was not significantly correlated with physical meat quality. However, in terms of eating experience, the minimum IMF level for better meat quality for consumer was 0.5%. Pearson correlation coefficients indicated that IMF content had significant negative correlations with moisture content but not with pH (r = -0.066), shear force (r = -0.072), WHC (r = 0.085), or color (L*(r = -0.049), a*(r = -0.028) and b*(r = 0.045)). Besides, meat pH had significant negative correlations with WHC (r = -0.036; P < 0.01) and lightness (L*) (r = -0.292; P < 0.01). Consequently, we consider pH to be one of the most important factors in evaluating meat quality of partridge.

5.
J Cell Physiol ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32017846

RESUMO

Chemokines and their receptors show a strong relationship with poor clinical outcomes in various cancers. However, their underlying mechanisms remain to be fully elucidated. In our research, we found C-C chemokine receptor 7 (CCR7) and its ligand chemokine ligand 21 (CCL21) were abnormally abundant in oral squamous cell carcinoma (OSCC) tissues, and CCR7 expression was correlated with poor prognosis of OSCC. After exogenous CCL21 stimulation, epithelial-mesenchymal transition (EMT) was promoted in OSCC cells, and cancer stem cell-related markers CD133, CD44, BMI1, ALDH1A1, and OCT4 increased. The migration, invasion, tumorsphere formation, and colony formation abilities of OSCC cells were enhanced, indicating that the stemness of OSCC cells was also improved. The knockdown and overexpression of CCR7 efficiently affected the CCL21-induced EMT and stemness of OSCC cells. When treated with CCL21, the phospho-JAK2 and phospho-STAT3 markedly increased. The inhibitor of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) significantly suppressed CCL21-induced EMT and stemness of OSCC cells. In conclusion, CCL21/CCR7 axis regulated EMT progress and promoted the stemness of OSCC by activating the JAK2/STAT3 signaling pathway. CCL21/CCR7 might be an effective target for OSCC prevention and treatment.

6.
Eur J Med Chem ; 190: 112079, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-32028140

RESUMO

A series of novel triptolide/furoxans hybrids were designed and synthesized as analogues of triptolide, which is a naturally derived compound isolated from the thunder god vine (Tripterygium wilfordii Hook. F). Some of these synthesized compounds exhibited antiproliferative activities in the nanomolar range. Among them, compound 33 exhibited both good antiproliferative activity and NO-releasing ability and the acute toxicity of compound 33 decreased more than 160 times (LD50 = 160.9 mg/kg) than triptolide. Moreover, compound 33 significantly inhibited the growth of melanoma at a low dose (0.3 mg/kg) and showed strong anti-inflammatory activity in vitro and in vivo. These results indicate that compound 33 could be a promising candidate for further study.

7.
Microb Pathog ; 142: 104043, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32032768

RESUMO

Cherax quadricarinatus is seriously affected by multiple types of pathogens, including bacteria and viruses, and has been widely transplanted around the world. Heat shock proteins (Hsps) are a group of molecular chaperones that play important roles in promoting the proper refolding and blocking the aggregation of denatured proteins. In this study, CqHsp60, CqHsp70 and CqHsp90 from C. quadricarinatus were cloned, and their expression patterns were analysed. The CDS (coding sequence) lengths of the CqHsp60, CqHsp70 and CqHsp90 genes were 1731 bp, 1932 bp and 2199 bp, encoding 576, 643 and 732 amino acids, respectively. CqHsp60 was 99.13%, 98.78% and 88.63% identical to the corresponding sequences of Cherax cainii, Cherax destructor and Eriocheir sinensis, respectively. CqHsp70 showed 99.84%, 92.73% and 91.58% identity to the corresponding sequences of C. cainii, C. destructor and E. sinensis, while CqHsp90 was 98.25%, 98.51% and 91.41% identical with those of C. cainii, C. destructor and E. sinensis, respectively. The expression patterns of the three CqHsps were different between males and females. CqHsp60 and CqHsp70 exhibited the highest expression in the hepatopancreas of males and the gonads of females, and CqHsp90 presented the highest expression in the gonads of males and hepatopancreas of females. After pathogenic inoculation, the death trend of C. quadricarinatus at different time points was the same in association with different pathogens, with most deaths occurring within 6 h post-inoculation. The trend of CqHsp transcription at different time points was the same among the groups treated with Vibrio alginolyticus, Vibrio parahemolyticus and Aeromonas hydrophila, exhibiting upregulation first and then downregulation. The expression of CqHsp60 and CqHsp70 in the gills of living C. quadricarinatus was less than 3.5 times that in the PBS group, but in the gills of dead C. quadricarinatus under A. hydrophila inoculation, its expression was more than 5-9 times that in the PBS group. CqHsp90 expression changed dramatically in the V. alginolyticus, V. parahemolyticus and A. hydrophila groups, in which it exceeded 50 times the level in the PBS group. These results indicated that CqHsps could induce the activation of the immune system within a short time and that CqHsp90 could be used as a more effective molecular biomarker than CqHsp70 and CqHsp60 in a pathogenic bacterium-polluted environment.

8.
Ultrason Sonochem ; 64: 104996, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32050142

RESUMO

Micro-nanobubbles (MNBs) generated during hydrodynamic cavitation (HC) have been extensively studied in mineral processing field in the past two decades. Many researchers have claimed that MNBs can effectively promote the collection of fine particles in flotation, while studies on MNBs assisted mineral separation are rare. In this study, the role of bulk MNBs in desorbing flotation reagent was investigated, with the aim of illustrating the potential effects of MNBs on minerals separation. The results showed that bulk MNBs could efficiently remove the sodium oleate (NaOl) from diaspore surfaces, reducing the residual concentration of NaOl on solids, which was more significant when the amount of NaOl pre-adsorbed was relatively small. Furthermore, lower residual concentration of NaOl on solids caused by MNBs cleaning made the particles less hydrophobic and flocs more friable. Given that gangue entrapment in flocs was one of the main limits for high-selective flotation, the roles of MNBs in enhancing reagent desorption and associated flocs breakup and reorganization probably contribute to higher separation efficiency of different minerals, which was confirmed by the flotation results of diaspore/kaolinite mixture.

9.
Artigo em Inglês | MEDLINE | ID: mdl-32041895

RESUMO

BACKGROUND: Epidemiologic studies have investigated the association between nut intake and risk for multiple cancers. However, current findings are inconsistent and no definite conclusion has been drawn from prospective studies. We therefore conducted this meta-analysis to evaluate the relationship between nut consumption and risk of cancer. METHODS: Prospective studies reporting associations between nut intake and risk for all types of cancer were identified by searching Web of Science and PubMed databases up to June 2019. Risk ratios (RR) and 95% confidence intervals (CI) were extracted and then pooled across the studies using a random-effect model. A dose-response analysis was modeled by performing restricted cubic splines when data were available. RESULTS: Thirty-three studies that included more than 50,000 cancer cases were eligible for the analysis. When comparing the highest with the lowest category of nut intake, high consumption of nuts was significantly associated with decreased risk of overall cancer (RR = 0.90; 95% CI, 0.85-0.95). The protective effect of nut consumption was especially apparent against cancers from the digestive system (RR = 0.83; 95% CI, 0.77-0.89). Among different nut classes, significant association was only obtained for intake of tree nuts. We also observed a linear dose-response relationship between nut consumption and cancer: Per 20 g/day increase in nut consumption was related to a 10% (RR = 0.90; 95% CI, 0.82-0.99) decrease in cancer risk. CONCLUSIONS: Our analysis demonstrated an inverse association of dietary nut consumption with cancer risk, especially for cancers from the digestive system. IMPACT: This study highlights the protective effect of nuts against cancer.

10.
Fish Physiol Biochem ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31993854

RESUMO

Type 1 diabetes is characterized by an increase in blood glucose levels resulting from damage to ß cells in pancreatic islets and the consequent absolute insufficiency of insulin. Animal models of type 1 diabetes were usually established using drugs toxic to ß cells, such as streptozotocin (STZ). To assess the application of zebrafish larvae in diabetes research, we explore the effects of STZ on pancreatic islets and glucose metabolism in zebrafish larvae. STZ was microinjected into the pericardial cavity of zebrafish larvae on alternate days for three times. At 2 days after the whole series of STZ injection (12 dpf), free-glucose level in larvae tissue shows a significant increase, and the fluorescence signal in immunohistochemistry, which indicates the insulin expression, was significantly weaker compared with the solution-injected control. Obvious apoptosis signals were also observed in the location of pancreatic islet, and insulin content decreased to be undetectable in STZ-injected larvae. Gene expression level of ins decreased to half of the solution injection control and that of casp3a was upregulated by 2.20-fold. Expression level of glut2 and gck decreased to 0.312-fold and 0.093-fold, respectively. pck1 was upregulated by 2.533-fold in STZ-injected larvae. By tracking detection, we found the free-glucose level in STZ-injected larvae gradually approached the level of the solution injection control and the insulin content recovered at 6 days post-STZ injection (16 dpf). Consistent with the change of the glucose level, the regeneration rate of the caudal fin in the STZ-injected group decreased initially, but recovered and accelerated gradually finally at 8 days post-amputation (20 dpf). These results indicate the generation of a transient hyperglycemia model due to ß-cell apoptosis caused by STZ, which is abated by the vigorous regeneration ability of ß cells in zebrafish larvae.

11.
Microb Biotechnol ; 13(2): 386-396, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31958883

RESUMO

Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species. Here, we report that the presence of an intron is necessary for the efficient expression of the heterologous phosphinothricin-resistance and green fluorescent protein genes in G. lucidum. Moreover, we improved the CRISPR/Cas9-mediated gene disruption frequency in G. lucidum by adding an intron upstream of the Cas9 gene. Our results showed that the disruption frequency of the orotidine 5'-monophosphate decarboxylase gene (ura3) in transformants containing the glyceraldehyde-3-phosphate dehydrogenase gene intron in the Cas9 plasmid is 14-18 in 107 protoplasts, which is 10.6 times higher than that in transformants without any intron sequence. Furthermore, genomic fragment deletions in the ura3 and GL17624 genes were achieved via a dual sgRNA-directed CRISPR/Cas9 system in G. lucidum. We achieved a ura3 deletion frequency of 36.7% in G. lucidum. The developed method provides a powerful platform to generate gene deletion mutants and will facilitate functional genomic studies in G. lucidum.

12.
Electrophoresis ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31945803

RESUMO

Prevention of infectious diseases, diagnosis of diseases, and determination of treatment options all rely on biosensors to detect and analyze biomarkers, which are usually divided into four parts: cell analysis, biochemical analysis, immunoassay, and molecular diagnosis. However, traditional biosensing devices are expensive, bulky, and require a lot of time to detect, which also limited its application in resource-limited areas. In recent years, Lab-on-PCB, which combines biosensing technology and PCB technology, has been widely used in biomedical applications due to its high integration, personalized design, and easy mass production. Among these Lab-on-PCB sensing devices, the PCB circuit plays an important role. It can be directly used as a resistance sensor to count cells, and also used as a control device to automatically control the detection device. Flexible PCBs can be used to make wearable medical biosensors. In addition, due to the high degree of integration of the PCB circuit, Lab-on-PCB can perform multiple inspections on the same platform, which reduces the inspection time equivalently. Therefore, in this review paper, we discuss the application of Lab-on-PCB in four analysis methods of cell analysis, biochemical analysis, immunoassay, and molecular diagnosis, and give some suggestions for improvement and future development trends at the end.

13.
Molecules ; 25(1)2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31948058

RESUMO

Grapefruit essential oil has been proven to have wide range of bioactivities. However, bioactivity of its molecular distillate has not been well studied. In this study, a light phase oil was obtained by molecular distillation from cold-pressed grapefruit essential oil and GC-MS was used to identify its chemical composition. The antimicrobial activity of the light phase oil was tested by filter paper diffusion method, and the anticancer activity was determined by the Cell Counting Kit-8 (CCK-8) assay. Twenty-four components were detected with a total relative content of 99.74%, including 97.48% of terpenes and 1.66% of oxygenated terpenes. The light phase oil had the best antimicrobial effect on Bacillus subtilis, followed by Escherichia coli, Staphylococcus aureus and Salmonellaty phimurium. DPPH and ABTS assays demonstrated that the light phase oil had good antioxidant activity. The CCK-8 assay of cell proliferation showed that the light phase oil had a good inhibitory effect on the proliferation of HepG2 liver cancer cells and HCT116 colon cancer cells.

14.
Oncol Lett ; 19(1): 1089, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31897222

RESUMO

[This corrects the article DOI: 10.3892/ol.2018.8971.].

15.
Neuroscience ; 429: 173-184, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31935490

RESUMO

Repeated induction of a temporary threshold shift (TTS) may result in a permanent threshold shift (PTS) and is thought to be associated with early onset of age-related hearing loss (ARHL). The possibility that a PTS might be induced by administration of repeated TTS-inducing noise exposures (NEs) over a short period during early adulthood has not been formally investigated. We aimed to investigate possible cumulative acoustic overstimulation effects that permanently shift the auditory threshold. Young adult C57BL/6J mice were exposed twice to moderate white noise in an experimental design that minimized the effects of aging. The first exposure resulted in a reversible noise-induced hearing loss (NIHL) measured as recoverable alterations in auditory brainstem response (ABR) thresholds, waveform amplitudes, and numbers of ribbon synapses. The second NE with the same parameters caused persistent threshold shifts, wave I amplitude reductions, wave IV/I ratio enhancements, and synaptic losses, even though recovery time sufficient for a TTS had been provided. The pattern of PTS resembled NIHL since the observed impairments tonotopically followed the power spectrum of the noise insult, rather than ARHL, which distributes at higher frequencies. No significant changes were observed in the control group as the mice aged. To conclude, our results demonstrate a cumulative effect of repetitive TTS-inducing NE on hearing function and synaptic plasticity that does not cause premature ARHL, thereby providing insight into the pathophysiological mechanisms underlying NIHL and ARHL.

16.
Theranostics ; 10(2): 797-815, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31903151

RESUMO

Background: Histone post-translational modifications (PTMs) are involved in various biological processes such as transcriptional activation, chromosome packaging, and DNA repair. Previous studies mainly focused on PTMs by directly targeting histone-modifying enzymes such as HDACs and HATs. Methods and Results: In this study, we discovered a previously unexplored regulation mechanism for histone PTMs by targeting transcription regulation factor 14-3-3ζ. Mechanistic studies revealed 14-3-3ζ dimerization as a key prerequisite, which could be dynamically induced via an allosteric effect. The selective inhibition of 14-3-3ζ dimer interaction with histone H3 modulated histone H3 PTMs by exposing specific modification sites including acetylation, trimethylation, and phosphorylation, and reprogrammed gene transcription profiles for autophagy-lysosome function and endoplasmic reticulum stress. Conclusion: Our findings demonstrate the feasibility of editing histone PTM patterns by targeting transcription regulation factor 14-3-3ζ, and provide a distinctive PTM editing strategy which differs from current histone modification approaches.

17.
Anal Chem ; 92(1): 1068-1073, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31820635

RESUMO

Portable, miniaturized, and inexpensive detectors are in high demand for detecting and discriminating volatile organic compounds (VOCs). Sensor array design and exploitation are two key issues for new detector development. In contrast to the most reported plane-shaped sensor array for gaseous analyte sensing, here we report a column-shaped fluorometric sensor array by using fluorophore-loaded silica particles (∼40 µm) filled capillary. In the design, the capillary serves as test chamber and facilitates visualization. The orifices of the capillary were used as inlet and outlet for gaseous analyte. Sensing modules are installed in series, which lays foundation for their even and effective contact with the gaseous analyte. Meanwhile, further capsulation could be avoided. Silica particles were chosen as carries due to their preferred adsorption behavior to VOCs. By choosing four typical fluorophores (PBI-CB, Py-CB-Ph, Py-At, and NA-Ch) as sensing units, a 4-element fluorometric sensor array was achieved. Fluorescence of the array varied when different alcohol vapors were pumped in. The six tested alcohols could not only be distinguished as primary, secondary, or tertiary, but also be identified individually. The array had good reproducibility in visualization of the six alcohols. In addition, the orders of the fluorophores can be changed as desired. It is believed that the proofed concept provides not only a totally new design of sensor array but also contributes a new strategy for the discrimination of the alcohols as examined.

18.
Bioorg Chem ; 94: 103435, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31812262

RESUMO

Two unique nitrogenous sesquiterpene quinone meroterpenoids, dysidinoid B (1) and dysicigyhone A (2), together with eight known analogues (3-10) were isolated and characterized from the marine sponge Dysidea septosa. Their structures with absolute configurations were established by a combination of extensive spectroscopic, electron circular dichroism (ECD) and single-crystal X-ray diffraction data analysis. Structurally, dysicigyhone A (2) possessed a unique benzo[d]oxazolidine-2-one unit. Additionally, dysidinoid B (1) exhibited significant anti-inflammatory effect by inhibiting TNF-α and IL-6 generation with IC50 values of 9.15 µM and 17.62 µM, respectively. Further in vivo anti-inflammatory assay verified that the dysidinoid B (1) alleviated the CuSO4-induced robust acute inflammatory response in zebrafish model.

19.
Dev Comp Immunol ; 104: 103535, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31697956

RESUMO

Karyopherin α4 (KPNA4) is an adaptor molecule that mediates type I interferon (IFN) production by facilitating the nuclear translocation of IFN transcription factors. Here, we cloned the duck KPNA4 (duKPNA4) gene and analyzed its involvement in type I IFN expression as well as antiviral response against Japanese encephalitis virus (JEV). The full-length duKPNA4 gene encoded a 520-amino acid protein that shared 97.3-98.7% sequence similarity with its orthologues in chickens, humans and mice. The duKPNA4 was extensively expressed in various duck tissues at the mRNA level. Analysis of the subcellular localization of duKPNA4 by immunofluorescence assays indicated that the duKPNA4 was primarily distributed in both the cytoplasm and nucleus in primary duck embryonic fibroblasts (DEFs). However, it translocated from the cytoplasm to the nucleus in response to poly(I:C) stimulation or JEV infection. The duKPNA4 interacted with duck IFN regulatory factor 7 and facilitated its nuclear translocation, thereby up-regulating the expression of IFN-α and IFN-ß in DEFs in the presence of poly(I:C) stimulation. Exogenous expression of duKPNA4 significantly elevated the expression of IFN-α and IFN-ß induced by JEV infection and inhibited JEV replication in DEFs. These data demonstrate the importance of duKPNA4 in type I IFN signaling as well as the antiviral response against JEV replication.

20.
J Adv Nurs ; 76(2): 600-610, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31736133

RESUMO

AIMS: To investigate workplace violence and nurse outcomes by comparing gender differences. DESIGN: A secondary analysis of cross-sectional survey data. METHODS: Workplace violence was measured by four items from the International Hospital Outcome Study. Nurse outcomes were measured by tools including burnout, job satisfaction and intention to stay. We used propensity score matching to generate a sociodemographic balanced dataset of 108 male and 288 female nurses. A hypothetical relationship model was derived from the affective events theory. Comparative statistics and multi-group structural equation modelling were conducted to analyze gender differences. Data were collected in China from December 2013 - August 2014. RESULTS: Male nurses reported more workplace violence from staff and less intention to stay than females. Besides finding the mediation of burnout sharing with female nurses consistent with the affective events theory, workplace violence was directly linked to less intention to stay in male nurses. CONCLUSION: Male nurses experience more workplace violence by staff than female nurses. Besides responding emotionally to workplace violence like female nurses, male nurses also respond behaviourally. IMPACT: What problem did the study address? Gender differences in workplace violence and its relationship to nurse outcomes. What were the main findings? Male nurses experienced more workplace violence than female nurses, linked directly to less intention to stay. Workplace violence linked to less job satisfaction and intention to stay in nurses was mediated by burnout. Where and on whom will the research have impact? Gender-based prevention of and coping with workplace violence should be included in nursing training.

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