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1.
J Nanosci Nanotechnol ; 20(2): 802-809, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383075

RESUMO

AgIO3/Ag2O/Ag nanocomposites with enhanced photocatalytic activities were synthesized by a onestep coprecipitation method at room temperature. The optimum hybrid of AgIO3/Ag2O = 1.25:1 with Ag nanoparticles (Ag NPs) loading (denoted as AA125) exhibited superior photocatalytic activity, demonstrating 97.19% tetracycline (TC) degradation within 60 min under simulated solar irradiation. This was approximately 10.44 and 2.63 times higher than that of pure Ag2O and AgIO3, respectively. The advanced photocatalytic activity can be ascribed to the synergetic effects of the heterostructured AgIO3/Ag2O/Ag and the strong surface plasmon resonance (SPR) effect of Ag NPs generated on the surface, which improved the separation and transfer efficiency of photoinduced electron-hole pairs. The results from radical scavenger experiments indicated that the degradation of TC was driven mainly by the participation of superoxide radical (·O-2).

2.
J Cell Physiol ; 235(1): 26-30, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31219174

RESUMO

Microtubule-interfering agents have been very useful both as biological tools in studying mitosis and as chemotherapeutic agents against cancer. It remains poorly understood how these agents converge on the spindle assembly checkpoint (SAC) to halt mitotic progression, while inhibiting microtubule dynamics by different mechanisms. Cells arrested at mitosis by various microtubule-interfering agents exhibit strikingly different defects in the mitotic spindle. However, all the arrested cells possess the 3F3/2 phosphoepitope at the sister kinetochores of chromosomes, indicating the decrease of tension across the paired kinetochores. In addition, microtubule-interfering agents result in a comparable reduction in the distance between sister kinetochores, suggesting that these agents decrease interkinetochore tension to similar degrees. Here, we discuss recent progress that suggests impairment of kinetochore-microtubule attachment and reduction of interkinetochore tension as common mechanisms underlying the persistent SAC activation in response to diverse microtubule-interfering agents.

3.
Medicine (Baltimore) ; 98(38): e17236, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31567987

RESUMO

BACKGROUND: The number of citations a published article receives can be used to demonstrate its impact on a field of study. The objective of this study was to identify and characterize the 100 most-cited research articles (T100) published on prenatal diagnosis. METHODS: The Web of Science (WOS) database was searched for papers on prenatal diagnosis published between 1900 and 2018. The 100 most-cited original articles and reviews were recorded. Each eligible paper was reviewed for authors, journal name, year of publication, country, institution, total citations, citation density, H-index, research field, article type, and keywords. RESULTS: The T100 were published between 1972 and 2015 with a mean of 332.7 citations per paper (range: 196-1254). Most of the T100 were published between 1990 and 2005, in 35 journals led by New England Journal of Medicine (n = 14) followed by Lancet (n = 10), and Proceedings of The National Academy of Sciences of the United States of America (n = 8). Studies on method application, which promotes field development, were the majority article type. The team of Lo YM featured prominently in the field, and the United States of America, United Kingdom, and Hong Kong, China were the leading countries/regions. Frequency of cooperation was also highest among these 3 regions. Hierarchical cluster analysis produced 4 groups of keywords. CONCLUSION: Our analysis provides a historical perspective on scientific progress in prenatal diagnosis and may assist clinicians and researchers in assessing the quality of research over the past 50 years. It also provides concise information to guide future research.


Assuntos
Bibliometria , Diagnóstico Pré-Natal , Feminino , Humanos , Gravidez
4.
J Diabetes Res ; 2019: 4845729, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31583249

RESUMO

Aims: To evaluate the performance of a factory-calibrated flash glucose monitoring system in children with diabetes compared to venous blood glucose (BG). Methods: A total of 13 hospitalized participants newly diagnosed with type 1 diabetes, aged 1~14 years old, were involved in the study. Sensor glucose measurements on days 2, 3, 6, 7, 12, and 13 of wear were compared with venous BG. During these days, the venous BG results were obtained either 4 or 7 times per day. Results: The accuracy was evaluated against venous BG, with 469 of 469 (100.0%) sensor and venous BG pairs within consensus error grid zones A and B, including 94.7% in zone A. The overall mean absolute relative difference (MARD) was 11.67%. The MARD of blood glucose lower than 4.0 mmol/L (MARD = 16.89%) was higher than blood glucose between 4 and 10 mmol/L (MARD = 11.58%) and blood glucose higher than 10 mmol/L (MARD = 7.79%). Compared to venous BG, the MARDs of wear days 2, 3, 6, 7, 12, and 13 were 11.53%, 9.66%, 11.79%, 10.89%, 13.18%, and 13.92%, respectively, with no statistically significant difference (P = 0.25). The median ARD was highest when the glucose decreased >0.11 mmol/L/min (20.27%) and lower than 10.00% when the glucose changed between 0.06 and 0.11 mmol/L/min, changed <0.06 mmol/L/min, and increased >0.11 mmol/L/min. Conclusions: The accuracy of the system is good and remains stable over 14 days of wear; however, the accuracy depends on the glucose level and rates of glucose concentration changes.

5.
Cancer Lett ; 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31589905

RESUMO

Due to a global increase in the incidence of thyroid cancer, numerous novel mouse models were established to reveal thyroid cancer pathogenesis and test promising therapeutic strategies, necessitating a comprehensive review of translational medicine that covers (i) the role of mouse models in the research of thyroid cancer pathogenesis, and (ii) preclinical testing of potential anti-thyroid cancer therapeutics. The present review article aims to: (i) describe the current approaches for mouse modeling of thyroid cancer, (ii) provide insight into the biology and genetics of thyroid cancers, and (iii) offer guidance on the use of mouse models for testing potential therapeutics in preclinical settings. Based on research with mouse models of thyroid cancer pathogenesis involving the RTK, RAS/RAF/MEK/ERK, PI3K/AKT/mTOR, SRC, and JAK-STAT signaling pathways, inhibitors of VEGFR, MEK, mTOR, SRC, and STAT3 have been developed as anti-thyroid cancer drugs for "bench-to-bedside" translation. In the future, mouse models of thyroid cancer will be designed to be ''humanized" and "patient-like," offering opportunities to: (i) investigate the pathogenesis of thyroid cancer through target screening based on the CRISPR/Cas system, (ii) test drugs based on new mouse models, and (iii) explore the underlying mechanisms based on multi-omics.

6.
Nat Commun ; 10(1): 4614, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601814

RESUMO

Autophagy is a central component of integrated stress responses that influences many inflammatory diseases, including inflammatory bowel disease (IBD) and colorectal cancer (CRC). While the core machinery is known, the molecular basis of the epigenetic regulation of autophagy and its role in colon inflammation remain largely undefined. Here, we report that BRG1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, is required for the homeostatic maintenance of intestinal epithelial cells (IECs) to prevent the inflammation and tumorigenesis. BRG1 emerges as a key regulator that directly governs the transcription of Atg16l1, Ambra1, Atg7 and Wipi2, which are important for autophagosome biogenesis. Defective autophagy in BRG1-deficient IECs results in excess reactive oxygen species (ROS), which leads to the defects in barrier integrity. Together, our results establish that BRG1 may represent an autophagy checkpoint that is pathogenetically linked to colitis and is therefore likely a potential therapeutic target for disease intervention.

7.
J Clin Lab Anal ; : e23069, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31602678

RESUMO

OBJECTIVE: To evaluate the diagnostic accuracy of cerebrospinal fluid (CSF)-based routine clinical examinations for post-neurosurgical bacterial meningitis (PNBM) in multicenter post-neurosurgical patients. METHODS: The diagnostic accuracies of routine examinations to distinguish between PNBM and post-neurosurgical aseptic meningitis (PNAM) were evaluated by determining the values of the area under the curve (AUC) of the receiver operating characteristic curve in a retrospective analysis of post-neurosurgical patients in four centers. RESULTS: An algorithm was constructed using the logistic analysis as a classical method to maximize the capacity for differentiating the two classes by integrating the measurements of five variables. The AUC value of this algorithm was 0.907, which was significantly higher than those of individual routine blood/CSF examinations. The predicted value from 70 PNBM patients was greater than the cutoff value, and the diagnostic accuracy rate was 75.3%. The results of 181 patients with PNAM showed that 172 patients could be correctly identified with specificity of 95.3%, while the overall correctness rate of the algorithm was 88.6%. CONCLUSIONS: Routine biomarkers such as CSF/blood glucose ratio (C/B-Glu), CSF lactate (C-Lac), CSF glucose concentration (C-Glu), CSF leukocyte count (C-Leu), and blood glucose concentration (B-Glu) can be used for auxiliary diagnosis of PNBM. The multicenter retrospective research revealed that the combination of the five abovementioned biomarkers can effectively improve the efficacy of the PNBM diagnosis.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31577406

RESUMO

Defect-engineering of semiconductors has been identified as an effective route towards enhancing the photoelectrochemical performances. There is a security threat in the traditional hydrogen-annealing process. In this work, oxygen defective nanofibers WO3-x/WO3 homojunction photoanode was in-situ synthesized via a novel metal self-reducing method. The as-prepared photoanode exhibits a 1.8-time higher solar water oxidation photocurrent density than that of bare WO3 film at 1.2 V vs. Ag/AgCl. The enhanced photoelectrochemical properties originate from the effective charge separation and injection, attributed to the stronger built-in electric field created by the oxygen-deficient homojunction. Importantly, this novel method is universal applicable to synthesize oxygen-deficient semiconductors materials, including films and powders.

9.
Nanotechnology ; 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31569084

RESUMO

We present the plasmon-enhanced linear and second-order surface nonlinear optical response of silver nanoparticles (Ag NPs) fabricated by using femtosecond pulse. Theoretical analysis indicates that the Ag NPs with diameter of ~100 nm has excellent linear response within visible band, and the electric field intensity enhancement factor reaches ~105 under excitation of continuous light of 632.8 nm. Meanwhile, the simulation result of second-order surface nonlinear optical response shows that the second harmonic conversion efficiency of Ag NPs dimer is two orders of magnitude higher than that of single Ag NP, under excitation of femtosecond pulse. In experiment, the linear response of Ag NPs is examined by using surface enhanced Raman spectroscopy (SERS) with Raman enhancement factor of ~1.7×1010, revealing the excellent linear optical response of Ag NPs. Moreover, the spectrum of second harmonic can be measured clearly under condition of the average pump power of 40 µW, revealing the excellent second-order surface nonlinear optical response of Ag NPs.

10.
Sensors (Basel) ; 19(19)2019 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-31569585

RESUMO

Localization technologies play an important role in disaster management and emergence response. In areas where the environment does not change much after an accident or in the case of dangerous areas monitoring, indoor fingerprint-based localization can be used. In such scenarios, a positioning system needs to have both a high accuracy and a rapid response. However, these two requirements are usually conflicting since a fingerprint-based indoor localization system with high accuracy usually has complex algorithms and needs to process a large amount of data, and therefore has a slow response. This problem becomes even worse when both the size of monitoring area and the number of reference nodes increase. To address this challenging problem, this paper proposes a two-level positioning algorithm in order to improve both the accuracy and the response time. In the off-line stage, a fingerprint database is divided into several sub databases by using an affinity propagation clustering (APC) algorithm based on Shepard similarity. The online stage has two steps: (1) a coarse positioning algorithm is adopted to find the most similar sub database by matching the cluster center with the fingerprint of the node tested, which will narrow the search space and consequently save time; (2) in the sub database area, a support vector regression (SVR) algorithm with its parameters being optimized by particle swarm optimization (PSO) is used for fine positioning, thus improving the online positioning accuracy. Both experiment results and actual implementations proved that the proposed two-level localization method is more suitable than other methods in term of algorithm complexity, storage requirements and localization accuracy in dangerous area monitoring.

11.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(8): 898-904, 2019 Aug 28.
Artigo em Chinês | MEDLINE | ID: mdl-31570677

RESUMO

OBJECTIVE: To explore the clinical characteristics, risk factors and factors affecting the severity of the disease in patients with diabetic foot at the current stage through a multi-center cross-sectional survey.
 Methods: Clinical data of 326 patients with diabetic foot (205 males and 121 females) from 13 general hospitals nationwide were collected from October to November 2017 using a unified clinical data collection table. The clinical characteristics were analyzed, and the influential factors for severe diabetic foot were analyzed by logistic regression analysis.
 Results: Among 326 patients with diabetic foot, 68.4% of the patients were more than 60 years old, and 60.1% of the patients received primary or junior high school education; 96.3% of the patients developed Type 2 diabetes; 80.1% of patients had glycated hemoglobin (HbA1c)≥7%; 60.1% of patients suffered dyslipidemia. Improper wearing of footwear (38.5%) is the main cause of diabetic foot. Diabetic neuropathy (76.7%), diabetic retinopathy (62.3%) and lower limb vascular disease (57.4%) were the most common complications. Logistic regression analysis showed that diabetic nephropathy, diabetic lower extremity vascular disease, and HbA1c levels were independent risk factors for severe diabetic foot, and receiving foot care education can be regarded as a protective factor.
 Conclusion: The diabetic foot occurs mostly in male patients, and Type 2 diabetes with older age, lower education level, poor glycemic control and dyslipidemia are the risk factors. Diabetic nephropathy, diabetic lower extremity vascular disease, HbA1c, and receiving foot care education are independent influential factors for the severity of diabetic foot.

12.
J Sci Food Agric ; 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31591721

RESUMO

BACKGROUND: Climate change has posed great challenges to rice production. Temperature and solar radiation show significant variations in central China. This study aims to analyze the responses of different rice genotypes to the variations of temperature and solar radiation in central China, and to find the way for identifying the optimal sowing date to improve and stabilize rice production. For this end, four rice genotypes (two Indica and two Japonica cultivars) were cultivated at two locations under irrigation conditions in two years with six sowing dates. RESULTS: We investigated variations of rice grain yield, resource use efficiency, average daily temperature and solar radiation during different phenological stages. Rice grain yield could increase by about 2% -17% in central China. Compared with solar radiation, temperature was a more important factor affecting rice grain yield in central China. The grain yield showed great correlation with the means temperature during different phenological stages, especially during the first 20 days after heading (GT20). Besides our results demonstrated that the grain yield displayed slighter variations when the GT20 was within 24.9-26.4°C. However, GT20 was higher than 26.4°C in most cases, which became more frequent due to climate changes. Analysis of climate change during the last 25 years revealed that the frequency of GT20 within 24.9-26.4°C was increased by the delay of sowing date. CONCLUSION: We propose that delaying sowing date to achieve the optimal GT20 (24.9°C-26.4°C) can be an effective strategy to stabilize and improve rice grain yield and resource use efficiency in central China. This article is protected by copyright. All rights reserved.

13.
Int J Biol Sci ; 15(10): 2087-2099, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31592124

RESUMO

Primary cilia and intraflagellar transport (IFT) proteins control a wide variety of processes during development and tissue homeostasis. However, their potential roles in the regulation of stem cell differentiation and tooth development remain elusive. Here, we uncovered the critical roles of ciliary IFT80 in cilia formation and differentiation of dental pulp stem cells (DPSCs). IFT80-deficient DPSCs showed reduced fibroblast growth factor receptor 1 (FGFR1) expression, leading to the disruption of FGF2-FGFR1 signaling. We found, during DPSC differentiation, FGF2-FGFR1 signaling induces stress fiber rearrangement to promote cilia elongation, meanwhile stimulates PI3K-AKT signaling to aid Hh/bone morphogenetic protein 2 (BMP2) signaling activation. These signaling pathways and their coupling were disrupted in IFT80-deficient DPSCs, causing impaired differentiation. Our findings revealed a novel mechanism that ciliary protein regulates the odontogenic differentiation of DPSCs through FGF/FGFR1 and Hh/BMP2 signaling.

15.
Neurol Sci ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31478148

RESUMO

Treatment with recombinant tissue plasminogen activator (rt-PA) is the most effective therapeutic option against brain ischemic stroke at the present time. However, elevated incidence of symptomatic intracerebral hemorrhage (SIH) greatly hinders ideal treatment outcome of rt-PA. We sought to assess the impacts of hesperidin on SIH following rt-PA therapies. Patients with ischemic stroke were assigned into two groups in a random fashion, to receive either rt-PA + placebo (Pc) or rt-PA + hesperidin. Treatment outcome was evaluated 24 h after the initial reperfusion using the transcranial Doppler ultrasonography (TCD) and the NIH Stroke Scale (NIHSS). Further, serum concentrations of transforming growth factor (TGF)-ß1, matrix metalloproteinase (MMP)-2, and MMP-9 were examined. Following the initial administration, stroke patients continued to receive either daily Pc or daily hesperidin, and the treatment outcome after 7 days was examined using the TCD, NIHSS, Glasgow Outcome Scale (GOS), and the Modified Rankin Scale (MRS). Combined treatment of rt-PA with hesperidin yielded significant improvement of outcomes, as revealed by better TCD and NIHSS scores as well as decreased SIH incidences, which could be attributable to elevation of TGF-ß1 and reduction in serum levels of both MMP-2 and MMP-9 caused by hesperidin. Follow-up hesperidin treatment for 7 consecutive days also markedly enhanced the recovery of stroke patients, as indicated by TCD, MRS, GOS, and NIHSS. Findings of the present study strongly suggested potential clinical application of hesperidin supplement in rt-PA therapies to reduce SIH and thereby improve the treatment outcomes of rt-PA in patients with ischemic stroke.

16.
Opt Express ; 27(17): 23830-23838, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31510282

RESUMO

Optical rogue waves emerge in nonlinear optical systems with extremely large amplitudes, and leave without a trace. In this work, we reveal the emergence of optical polarization rogue waves in supercontinuum generation from a zero-dispersion fiber, pumped by a dissipative soliton laser. Flat spectral broadening is achieved by modulation instability, followed by cascaded four-wave-mixing. In this process, we identify the emergence of optical polarization rogue waves, based on the probability density function of the relative distance among polarization states. Experimental results show that optical polarization rogue waves originate from vector multi-wave-mixing. Besides, we observe double peaks, and even triple peaks in the histogram of the state of polarization. This is a new and intriguing property, never observed so far in optical rogue waves, for example those emerging in the statistics of pulse intensities. Our polarization domain statistical analysis provides a new insight into the still debated topic of the mechanism for rogue wave generation in optical supercontinuum.

17.
Compr Psychiatry ; 94: 152121, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31476590

RESUMO

BACKGROUND: Methylenetetrahydrofolate reductase (MTHFR) is the critical enzyme in biotransformation. The polymorphism of MTHFR is a risk factor for schizophrenia. However, whether the MTHFR polymorphism is associated with schizophrenia disease phenotypes and what is the underlying mechanism of MTHFR polymorphism in schizophrenia is under-investigated. In this study, we aim to verify the correlation between MTHFR polymorphisms and clinical features of schizophrenia, while exploring the differential genomic methylation and disease related genes as the potential targets for schizophrenia. METHOD: 242 patients of schizophrenia and 234 matched healthy controls were enrolled in this study. Polymorphisms of MTHFR from three sites (C677T, A1298C, G1793A) were examined by Taqman fluorescence probe method in leukocytes from all subjects. The positive and negative syndrome scale (PANSS), trail making test (TMT) and Clinical Global Impression (CGI) were checked on patients. Genomic methylation was tested and analyzed in fields of differential methylation positions (DMPs) and enrichment of genes that are potentially related to schizophrenia. RESULTS: Schizophrenic patients showed higher frequency of MTHFR polymorphisms at both single and multiple sites than healthy controls. Our data also showed that MTHFR C677T and multiple-site polymorphisms were positively correlated with PANSS positive rating, not negative score in male schizophrenia patients. Furthermore, while a significant reduction of global DNA methylation level was observed in schizophrenic patients, we also identified several genes which differentiated between schizophrenia and healthy controls at methylation levels. CONCLUSIONS: This is a pilot study revealing that MTHFR polymorphisms at both single and multiple sites are related to the risk of schizophrenia and positive symptom of the disease. The risk of MTHFR polymorphism in schizophrenia and the clinical symptoms was only significant in male patients. While the sex-specific risk of MTHFR in schizophrenia is new and the reasons remain unanswered. Our methylation analysis suggested that there was significant hypomethylation of genomic DNA in schizophrenia patients with no sex difference. The correlation between MTHFR polymorphism and schizophrenia may attribute to the change of DNA methylation level, and some certain genes could be potential research objects for MTHFR effects on schizophrenia.

18.
Chemosphere ; 239: 124678, 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31494323

RESUMO

In the developing countries such as China, most well-developed areas have suffered severe haze pollution, which was associated with increased premature morbidity and mortality and attracted widespread public concerns. Since ground-based PM2.5 monitoring has limited temporal and spatial coverage, satellite aerosol remote sensing data has been increasingly applied to map large-scale PM2.5 characteristics through advanced spatial statistical models. Although most existing research has taken advantage of the polar orbiting satellite instruments, a major limitation of the polar orbiting platform is its limited sampling frequency (e.g., 1-2 times/day), which is insufficient for capturing the PM2.5 variability during short but intense heavy haze episodes. As the first attempt, we quantitatively investigated the feasibility of using the aerosol optical depth (AOD) data retrieved by the Geostationary Ocean Color Imager (GOCI) to estimate hourly PM2.5 concentrations during winter haze episodes in the Yangtze River Delta (YRD). We developed a three-stage spatial statistical model, using GOCI AOD and fine mode fraction, as well as corresponding monitoring PM2.5 concentrations, meteorological and land use data on a 6-km modeling grid with complete coverage in time and space. The 10-fold cross-validation R2 was 0.72 with a regression slope of 1.01 between observed and predicted hourly PM2.5 concentrations. After gap filling, the R2 value for the three-stage model was 0.68. We further analyzed two representative large regional episodes, i.e., a "multi-process diffusion episode" during December 21-26, 2015 and a "Chinese New Year episode" during February 7-8, 2016. We concluded that AOD retrieved by geostationary satellites could serve as a new valuable data source for analyzing the heavy air pollution episodes.

19.
Sci Total Environ ; 698: 134298, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31505343

RESUMO

Many studies have examined the acute toxicity of nanoparticles (NPs) towards model bacteria. In this study, we report the time-dependent effects of ZnO NPs on native, selected Zn-resistant and dominant bacteria in estuarine waters. An initial inhibition of bacterial growth followed by a recovery at 24 h was observed, and this rebound phenomenon was particularly notable when the raw water samples were treated with relatively high ZnO NP concentrations (1 and 10 mg/L).By comparing the groups treated with Zn2+, Zn2+ was shown to largely explain the acute cytotoxic effect of ZnO NPs on bacteria in raw waters. Furthermore, similar to the native bacteria, especially the dominant bacteria, the viability of Escherichia coli (E. coli) decreased with the increasing treatments time and the concentrations of ZnO NPs in water with different salinities. Moreover, the expression of Zn-resistance genes including zntA and zntR in E. coli suggested that the Zn-resistance system in E. coli can be activated to defend against the stress of Zn2+ released from ZnO NPs, and salinity may promote this process in estuarine aquatic systems. Thus, the effect of ZnO NPs on bacteria in estuarine water bodies is likely determined by the synergistic effect of environmental salinity and dissolved Zn ions. As such, our findings are of high relevance and importance for understanding the ecological disturbances caused by anthropogenic NPs in estuarine environments.

20.
Int J Biol Macromol ; 141: 345-350, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31491517

RESUMO

Natural resistance associated macrophage protein genes (Nramp) is one of the important candidate genes responsible for regulating immune response against pathogen infection. The aim of the present was to quantify expression of Nramp gene in response to pathogen infection. Here, a Nramp was identified and molecularly characterized from Pelteobagrus fulvidraco (PfNramp). The obtained 3134 bp cDNA fragment of PfNramp comprised a 5'-untranslated region (UTR) of 81 bp, a 3'-UTR of 1403 bp and an open reading frame (ORF) of 1650 bp, encoding a polypeptide of 549 amino acids that contained a typical structural features of Nramp domain (Pfam01566). BLAST analysis exhibited that PfNramp shared sequence similarity to other organisms, in particular to Ictalurus furcatus (92%), Danio rerio (82%), and Homo sapiens (77%). Phylogenetic analysis revealed that PfNramp is close to Teleostei. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis showed that PfNramp was expressed in all examined tissues, with the highest abundance in liver. The mRNA expression of PfNramp was remarkably increased at different time points after lipopolysaccharide (LPS), and polyriboinosinic polyribocytidylic acid (poly I:C) challenge. These results suggest that PfNramp is an inducible protein in the innate immune reactions of P. fulvidraco and probably in other fish species.

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