Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.896
Filtrar
1.
Methods Mol Biol ; 2081: 177-190, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31721125

RESUMO

Pseudoviruses are useful tools because of their safety and versatility compared to wild type viruses. Optical imaging of reporter gene labeled pseudoviruses in small animal models can allow for real-time analysis of the infection process without sacrificing the host, which has proven invaluable in the longitudinal study of disease events and testing the antiviral efficiencies of vaccine candidates, monoclonal antibodies and small molecule compounds. Here, we describe the generation of Marburg pseudovirus (pMARV) and establishment of imaging mouse model by using a deep-cooled CCD camera imager. We also describe the widespread organ distribution of pMARV during infection by ex vivo imaging of necropsied tissues. This system can significantly facilitate Marburg virus studies and enable the evaluation of treatments against MARV in BSL-2 containments.

2.
Cytokine ; 125: 154853, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31557634

RESUMO

Immune thrombocytopenia (ITP) is an autoimmune disease characterized by low platelet count with heterogeneous bleeding manifestations. Severe bleeding in ITP is not completely related with low platelet count. Fcγ receptor (FcγR)-mediated platelet destruction is one of the major mechanisms of ITP. Interleukin-37 (IL-37) is a fundamental natural suppressor of innate immunity and inflammatory responses in several autoimmune diseases. However, the role of IL-37 in the pathogenesis of ITP is unknown. In the present study, we identified that IL-37 expression was elevated in ITP patients, which was correlated with platelet count and the severity of bleeding in ITP, indicating that IL-37 could be a candidate in evaluating disease severity of ITP. In the in vitro study, IL-37 initiated an anti-inflammatory effect on monocytes/macrophages from ITP patients by down-regulating the phosphorylation of MAPK, AKT, and NF-κB signaling pathways. Moreover, IL-37 restored the balance of activating and inhibitory FcγRs and decreased antibody-mediated platelet phagocytosis by monocytes/macrophages. Our findings suggest that IL-37 may be a promising indicator of the disease severity and supplementation of IL-37 may be therapeutically beneficial for ITP patients.

3.
J Cell Biochem ; 121(1): 49-62, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31571264

RESUMO

Acute coronary syndrome (ACS) is characterized by atherosclerotic plaque rupture with a high incidence of recurrent ischemic events. Several microRNAs are found to be aberrantly expressed in atherosclerotic plaques. This study aims to investigate the effects of microRNA-9 (miR-9) on vulnerable atherosclerotic plaque and vascular remodeling in ACS and underlying mechanisms. Microarray-based gene expression profiling was used to identify differentially expressed genes related to ACS and regulatory miRNAs. Oxidized low-density lipoprotein (lectin-like) receptor 1 (OLR1) was identified to be aberrantly activated in ACS and regulated by miR-9. OLR1 was verified as a target gene of miR-9 by bioinformatics prediction and dual luciferase reporter gene assay. The atherosclerotic models were induced in ApoE-/- mice, in which the agomir or antagomir of miR-9, or small interfering RNA (siRNA) against OLR1 were separately introduced. Serum lipid levels and expression of vascular remodeling and inflammatory response-related factors were determined, respectively. On the basis of the obtained results, in the atherosclerosis mice treated with the agomir of miR-9 and siRNA against OLR1, the p38-mitogen-activated protein kinase (p38MAPK) pathway was inhibited; levels of triglyceride, total cholesterol, low-density lipoprotein cholesterol, tumor necrosis factor-α, interleukin-6, and vascular endothelial growth factor were reduced, but the high-density lipoprotein cholesterol level was increased, along with decreased vulnerable atherosclerotic plaque area and enhanced vascular remodeling. Taken together, these findings suggested an inhibitory role miR-9 acts in the formation of vulnerable atherosclerotic plaques in ACS mice, along with a promoted vascular remodeling, via a negative feedback regulation of OLR1-mediated p38MAPK pathway.

4.
Sci Total Environ ; 701: 135036, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31710901

RESUMO

The catalytic activity of a new catalyst for NO reduction is usually obtained by a gas-solid phase experiment. For the first time, a relationship has been established between catalytic performance and electrochemistry property. A bimetallic catalyst showed a low-temperature activity and removed 67.27% of NO at 300 °C, 33.39% bigger than a single-metallic catalyst. The bimetallic catalyst removed 9.770 mmol/g of NO after 1440 min, 5.212 mmol/g bigger than the single-metallic one. At the same time, the bimetallic catalyst showed a reduction capacity of 0.441 mmole/g. In comparison, the single-metallic one only had 0.242 mmole/g. Moreover, the limiting current of bimetallic catalyst (4.020 e-4A) was also bigger than that of single-metallic one (3.698 e-4A). Therefore, a catalyst for NO reduction showed better catalytic activity and electron-transfer ability at the same time. In other words, the catalytic activity of a catalyst can be potentially estimated by detecting its electrochemistry properties.

5.
Urol Oncol ; 38(1): 5.e17-5.e23, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31672484

RESUMO

OBJECTIVES: It remains unclear whether the immunologic status of cells in peripheral blood can be used as a prognostic indicator of response to treatment for patients with neoadjuvant chemotherapy (NAC). This study sought to evaluate whether the proportion of granulocytic myeloid-derived suppressor cells (G-MDSCs) and monocytic myeloid-derived suppressor cells could correlate with pathologic response in bladder cancer patients receiving NAC. PATIENTS AND METHODS: Pretreatment peripheral blood levels of G-MDSCs and monocytic myeloid-derived suppressor cells were measured by flow cytometry. We divided patients into high and low (above and below the median, respectively) groups based on the median value for each immune cell subset and compared outcomes of the two groups. RESULTS: A significant pathological response (pT0-1) was attained in 13% (6 of 45) of patients with high G-MDSCs compared with 58% (26 of 45) of patients with low G-MDSCs (P < 0.001). Patients with high G-MDSCs had significantly shorter disease specific survival and progression-free survival (both P < 0.001). In the multivariate analysis for survival, high G-MDSCs and pathological response emerged as independent prognostic factor for progression-free survival (P < 0.001 and P = 0.017) and disease-specific survival (P < 0.001 and P = 0.014). CONCLUSIONS: Pretreatment peripheral G-MDSCs may represent a potential marker for the outcome of patients treated with cisplatin-based NAC.

6.
J Cell Physiol ; 235(2): 1615-1623, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31347168

RESUMO

The incidence of gallbladder cancer (GBC) is relatively rare but a high degree of malignancy. The migration and invasion potential of GBC severely affects the prognosis of patients with GBC. Glycochenodeoxycholate (GCDC) is one of the most important components in GBC-associated microenvironment. However, the role of GCDC in the metastatic feature of GBC cells is not fully understood. First, the results of this study found that GCDC could effectively enhance the metastasis of GBC cells. Furthermore, GCDC could lead to the enhancement of epithelial to mesenchymal transition (EMT) phenotype in GBC cells, which is concerned to be an important mechanism of tumor metastasis. Further studies showed that GCDC treatment induced the upregulation of matrix metalloproteinase-3 (MMP3), MMP9, and SOCS3/JAK2/p-STAT3 signal pathway in GBC cells, which could regulate the level of EMT. Beside that, we also found the positive expression of farnesoid X receptor (FXR) in GBC cells and inhibition of FXR could significantly block the effect of GCDC on the metastasis of GBC cells. These results indicated that GCDC promoted GBC cells metastasis by enhancing the level of EMT and inhibition of FXR could significantly block the effect of GCDC. On one hand, FXR might be an indicator for predicting the metastasis of patient with GBC. On the other hand, FXR might serve as a potential antimetastasis target in GBC therapy.

7.
J Hazard Mater ; 383: 121151, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31678744

RESUMO

Green Zn-based spinel sorbents for hot coal gas desulfurization have been developed with the assistance of optimization procedures. The pilot study highlights an outstanding ordered mesoporous support (SBET = 323 m2 g-1, Da = 4.3 nm) of SAPO-34@as-prepared SBA-15 (SS) for loading active metal oxides. ZnCo2O4 spinel loaded onto SS (ZnCo2/SS) exhibits a prominent desulfurization performance compared to other sorbents whose partial Co is substituted by Mn or Fe in spinel B-site, owing to the slight effect of PO43- in SS. After systematic evaluation on role of sulfidation condition, 50 wt% ZnCo2/SS sorbent possesses the sulfur storage capacity of 138.08  mg g-1at550 °C and little loss of active species in 5 desulfurization-regeneration cycles. Results of high resolution transmission electron microscopy (HRTEM), Brunauer-Emmett-Teller (BET), X-ray photoelectron spectroscopy (XPS) etc. demonstrate that 70.42% of initial sulfur capacity of ZnCo2/SS presented in the 2nd utilization is associated with zinc evaporation, existence of high stable sulfides and partial sintering. The improved deactivation kinetic model suitably describes that the H2S concentration distribution relates with the spatial position of fixed-bed reactor and desulfurization time.

8.
Org Biomol Chem ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793614

RESUMO

The ring-opening of cyclopropanols is one of the most active areas of research and it has been well documented in recent years owing to subsequent coupling with various partners, thus providing the facile syntheses of a large number of multifunctional compounds that may otherwise be difficult to access. Evidently, the useful cascade reaction requires easy access to diversely functionalized cyclopropanol substrates. However, developments in the construction of cyclopropanols have not received adequate attention. Herein, recent reports on the formation of cyclopropanols are summarized, and the highly stereoselective production of new promising substrates for the cyclopropanol ring-opening/cross-coupling reactions are introduced and improved syntheses of known cyclopropanols are depicted. This review may facilitate more interesting applications of the cyclopropanol ring-opening/coupling reaction in the synthesis of pharmaceutical compounds, natural products, and structurally more diversified organic synthetic intermediates.

9.
Cancer Biomark ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31796663

RESUMO

BACKGROUND: Esophageal cancer is a common tumor with high mortality worldwide. In the present study, we aimed to investigate the prognostic significance and regulatory effects of miR-105 on cellular functions of esophageal cancer cells. METHODS: The expression level of miR-105 was analyzed in esophageal cancer tissues and cell lines by qRT-PCR. Survival analysis was carried out using the Kaplan-Meier and the prognostic significance of miR-105 was analyzed with Cox regression analysis. The effects of miR-105 on cell proliferation, migration, and invasion abilities were detected with cellular experiments. RESULTS: We found that miR-105 was significantly upregulated in esophageal cancer tissues and cell lines, compared with the control group, respectively. Moreover, overexpression of miR-105 was significantly associated with positive lymph node metastasis, advanced TNM stage, and poor overall survival. In addition, overexpression of miR-105 promoted cell proliferation, migration, and invasion in esophageal cancer cells, while downregulation of miR-105 suppressed these cellular behaviors. CONCLUSION: Our findings indicate that a higher level of miR-105 predicts poorer prognosis in esophageal cancer patients, and miR-105 can promote esophageal cancer cell proliferation, migration, and invasion.

10.
Front Immunol ; 10: 2576, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31787973

RESUMO

Cigarette smoke is a major preventable risk factor of ischemic stroke. Cigarette smoke induces a significant increase in circulating leukocytes. However, it remains unclear to what extent and by what mechanisms smoke priming influences stroke severity. Here we report that exposure to cigarette smoke exacerbated ischemic brain injury in mice subjected to transient middle cerebral artery occlusion (MCAO). The augmentation of neurodeficits and brain infarction was accompanied by increased production of pro-inflammatory factors and brain infiltration of neutrophils and monocytes. Prior to brain ischemia, exposure to cigarette smoke induced mobilization of peripheral neutrophils, and monocytes. Furthermore, the detrimental effects of smoke priming on ischemic brain injury were abolished either by pharmacological inhibition of the recruitment of neutrophils and monocytes or by blockade of the NLRP3 inflammasome, an effector protein of neutrophils and monocytes. Our findings suggest that cigarette smoke-induced mobilization of peripheral neutrophils and monocytes augments ischemic brain injury.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31791582

RESUMO

Neural crest stem cells (NCSCs) often referred to as the fourth germ layer, comprise a migratory, stem and progenitor cell population and are synonymous with vertebrate evolution and development. The cells follow specific paths to migrate to different locations of the body where they generate a diverse array of cell types and tissues. There are NCSCs which are maintained in an undifferentiated state throughout the life in the animal tissues. Based on some cells migratory property, we successfully developed a separation strategy to isolate and identify a population of adipose-derived stem cells with neural crest stem cell features in adult bovine adipose tissues within minimally-invasive surgical procedures. The cells have a high degree of multi-potency and self-renewal capabilities, can be cultured and maintained in feeder-free adhesion conditions as monolayer cells, and also be able to grow in the suspension condition in the form of neurosphere. For the purpose of simple description, we name this type cell as bovine adipose-derived neural crest stem cell (baNCSC). Taken together our study describes a readily accessible source of multipotent baNCSC for autologous tissue engineer and cell-based therapeutic researches.

12.
Nat Commun ; 10(1): 5492, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792210

RESUMO

Gemcitabine is the first-line treatment for locally advanced and metastatic gallbladder cancer (GBC), but poor gemcitabine response is universal. Here, we utilize a genome-wide CRISPR screen to identify that loss of ELP5 reduces the gemcitabine-induced apoptosis in GBC cells in a P53-dependent manner through the Elongator complex and other uridine 34 (U34) tRNA-modifying enzymes. Mechanistically, loss of ELP5 impairs the integrity and stability of the Elongator complex to abrogate wobble U34 tRNA modification, and directly impedes the wobble U34 modification-dependent translation of hnRNPQ mRNA, a validated P53 internal ribosomal entry site (IRES) trans-acting factor. Downregulated hnRNPQ is unable to drive P53 IRES-dependent translation, but rescuing a U34 modification-independent hnRNPQ mutant could restore P53 translation and gemcitabine sensitivity in ELP5-depleted GBC cells. GBC patients with lower ELP5, hnRNPQ, or P53 expression have poor survival outcomes after gemcitabine chemotherapy. These results indicate that the Elongator/hnRNPQ/P53 axis controls gemcitabine sensitivity in GBC cells.

13.
Sci Rep ; 9(1): 17262, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754257

RESUMO

Photoacoustic imaging has the advantages of high contrast and deep imaging depth. However, with the increasing of imaging depth, the signal-to-noise ratio (SNR) of the detected signal decreases, due to the light scattering that seriously affects the recovery image quality. In this paper, we experimentally demonstrated that higher contrast photoacoustic imaging was achieved using photoacoustic wavefront shaping technology in the presence of light scattering and low SNR signals. The imaging contrast is improved from 1.51 to 5.30. More importantly, we propose a dynamic time window method for the photoacoustic signal extraction algorithm, named correlation detection of adaptive time window, which further improves the contrast of photoacoustic imaging to 9.57. Our method effectively improves the contrast of photoacoustic imaging through scattering media.

14.
Opt Express ; 27(20): 27873-27881, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684548

RESUMO

A common-path dual-wavelength phase demodulation technique for extrinsic Fabry-Perot interferometric (EFPI) sensors is proposed on the basis of a broadly tunable modulated grating Y-branch (MG-Y) laser. It can address the three main concerns of existing dual-wavelength phase interrogation methods: the imbalances and disturbances caused by two optical paths utilizing two lasers or two photodetectors, the restrictions between two operating wavelengths and the cavity length of EFPI, and the difficulty in eliminating the direct current (DC) component of the interferometric fringe. Dual-wavelength phase interrogation is achieved in a common optical path through high-speed wavelength switching. Taking advantage of the MG-Y laser's full spectrum scanning ability (1527 ∼ 1567 nm), initial cavity length and DC component can be directly measured by white light interferometry. Two quadrature wavelengths are then selected to perform high speed phase demodulation scheme. Three polyethylene terephthalate (PET) diaphragm based EFPI acoustic sensors with cavity lengths of 127.954 µm, 148.366 µm and 497.300 µm, are used to demonstrate the effectiveness.

15.
Elife ; 82019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31718773

RESUMO

The central nervous system transforms sensory information into representations that are salient to the animal. Here we define the logic of this transformation in a Caenorhabditis elegans integrating interneuron. AIA interneurons receive input from multiple chemosensory neurons that detect attractive odors. We show that reliable AIA responses require the coincidence of two sensory inputs: activation of AWA olfactory neurons that are activated by attractive odors, and inhibition of one or more chemosensory neurons that are inhibited by attractive odors. AWA activates AIA through an electrical synapse, while the disinhibitory pathway acts through glutamatergic chemical synapses. AIA interneurons have bistable electrophysiological properties consistent with their calcium dynamics, suggesting that AIA activation is a stereotyped response to an integrated stimulus. Our results indicate that AIA interneurons combine sensory information using AND-gate logic, requiring coordinated activity from multiple chemosensory neurons. We propose that AIA encodes positive valence based on an integrated sensory state.

16.
Int J Clin Oncol ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31720994

RESUMO

OBJECTIVE: PBRM1, located on 3p21, functions as a tumor suppressor and somatic mutation of PBRM1 is frequent in clear cell renal cell carcinoma (ccRCC). This study aims to determine the influence of PBRM1 expression on the prognosis of patients with mRCC receiving tyrosine kinase inhibitor (TKI) treatment. METHODS: We identified 116 mRCC patients who were administered sunitinib or sorafenib as first-line therapy, between January 2006 and December 2016 at our institution. PBRM1 expression was assessed by immunohistochemistry. The Kaplan-Meier method was used to estimate the progression-free survival (PFS) and overall survival (OS), log-rank test was used to compare the survival outcomes between patients with low and high PBRM1 expression levels, and the Cox proportional hazard regression model was used to estimate the prognostic value. Prognostic accuracy was determined using Harrell concordance index, and nomograms were built to evaluate the prognosis of mRCC. RESULTS: Patients with low PBRM1 expression had significantly shorter median PFS (9 vs 26 months, P < 0.001) and OS (21 vs 44 months, P < 0.001) than those with high expression. Multivariate analysis showed that PBRM1 expression was an independent predictor of PFS (HR 1.975, P = 0.013) and OS (HR 2.282, P = 0.007). The model built by the addition of PBRM1 improved the C-index of PFS and OS to 0.72 and 0.82, respectively. CONCLUSIONS: The expression of PBRM1 could be a significant prognostic factor for mRCC patients treated with targeted therapy, and it increases the prognostic accuracy of the established prognostic model.

17.
Sci Total Environ ; 695: 133787, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31756871

RESUMO

Satellite-derived terrestrial latent heat flux (LE) models are useful tools to understand regional surface energy and water cycle processes for terrestrial ecosystems in the Heihe River basin (HRB) of Northwest China. This study developed a satellite-derived hybrid LE model parameterized by three soil moisture (SM) constraints: SM, relative humidity (RH), and diurnal air temperature range (DT); and assessed model performance and sensitivity. We used MODerate Resolution Imaging Spectroradiometer (MODIS) and eddy covariance (EC) data from 12 EC flux tower sites across the HRB. The hybrid model was trained using observed LE over 2012/2013-2014, and validated using observed LE for 2015 and leave-one-out cross-validation. The results show that the three SM constraints schemes exhibited some modeling differences at the flux tower site scale. LE estimation using SM achieved the highest correlation (R2 = 0.87, p < 0.01) and lowest root mean square error (RMSE = 20.1 W/m2) compared to schemes using RH or DT schemes. We then produced regional daily LE maps at 1 km × 1 km across the HRB for 2013-2015. Regional analysis shows that our LE estimates from all three constraint models exhibited large spatial variability and strong seasonal and annual variations, attributed to differences in parameterizing the model water constraints. This study provides data and model based evidence to improve satellite-derived hybrid LE models with regard to water constraints.

18.
Hum Vaccin Immunother ; : 1-14, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31687875

RESUMO

Chimigen® HBV Immunotherapeutic Vaccine (C-HBV), a recombinant chimeric fusion protein comprising hepatitis B virus (HBV) S1 and S2 surface antigen fragments, Core antigen and a murine monoclonal antibody heavy chain fragment (Fc), was designed and produced in Sf9 insect cells. C-HBV targets the host immune system through specific receptors present on dendritic cells (DCs) which facilitates antigen internalization, processing, and presentation on MHC class I and II to induce both cellular and humoral immune responses against HBV antigens. T cell responses, previously assessed by ex vivo antigen presentation assays using human peripheral blood mononuclear cell (PBMC)-derived DCs and T cells from uninfected and HBV chronic-infected donors, demonstrated that C-HBV was highly immunogenic. A vaccine dose response study was performed in sheep to analyze the immunogenicity of C-HBV in vivo. Sheep (n = 8/group) received three consecutive subcutaneous injections of each dose of C-HBV at four-week intervals. Analysis of serum antibody levels confirmed C-HBV induced a dose-dependent antibody response to C-HBV and S1/S2-Core. Kinetics of the S1/S2-Core specific antibody response was similar to hepatitis B surface antigen (HBsAg)-specific antibody responses induced by ENGERIX-B. Analysis of cell-mediated immune responses (CMI) confirmed C-HBV induced both dose-dependent S1/S2-Core-specific lymphocyte proliferative responses and IFN-γ secretion. These responses were stronger with blood lymphocytes than with cells isolated from the lymph node draining the vaccination site. No correlation was seen between antibody titers and CMI. The results confirm C-HBV is an effective delivery vehicle for the induction of T cell responses and may be an appropriate candidate for immunotherapy for chronic HBV infections.

19.
Biomed Res Int ; 2019: 7081032, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31737674

RESUMO

Objective: The aim of this study was to compare the functional outcome and complications in midshaft clavicle fractures receiving minimally invasive plate osteosynthesis and conventional open plating. Methods: Relevant studies were searched in the databases of Medline, EMBASE, Cochrane Library, Ovid, and Web of Science from inception to March 1, 2019. Pooled data were analyzed with Cochrane Collaboration's Review Manager 5.3. Results: A total of 7 studies were included, of which 2 were randomized controlled trials, 3 were retrospective cohort studies, and 2 were prospective cohort studies including 316 patients. No statistical differences in functional outcome (weighted mean difference [WMD] = 0.99, P = 0.12), operation time (WMD = -10.44, P = 0.07) and time to bone union (WMD = -0.23, P = 0.70) were observed between the two groups. However, minimally invasive plate osteosynthesis reduced rates of skin numbness (odds ratio (OR) = 0.25, 95% CI : 0.13 to 0.48; P < 0.0001) and complications (OR = 0.33, 95% CI : 0.16 to 0.71; P = 0.005) compared with conventional open plating. Conclusion: This systematic review and meta-analysis found no differences in terms of functional outcomes, operation time, and fracture healing time between minimally invasive plate osteosynthesis and conventional open plating. However, minimally invasive plate osteosynthesis had apparent advantages in rates of skin numbness and complications.

20.
Analyst ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31750449

RESUMO

Different from the classical antiparallel DNA double-stranded structure, parallel DNA duplexes possess unique structures and potential biological functions. In this work, we found that the parallel DNA homoduplex from the [TG(GA)3]n sequence ([TG(GA)3]n-dsDNA) can dramatically enhance the fluorescence of thioflavin T (ThT), and the fluorescence enhancement is proportional to the number (n) of TG(GA)3 units in [TG(GA)3]n. Compared with the traditional G-quadruplex/ThT system, [TG(GA)3]n/ThT showed more stable and stronger fluorescence emission. In addition, coupled with an isothermal exponential amplification reaction, [TG(GA)3]3/ThT was used as a label-free fluorescent probe to detect microRNA, and the [TG(GA)3]3/ThT probe exhibited higher sensitivity than the G-quadruplex/ThT probe. This work provides a new paradigm to design label-free fluorescent biosensing/imaging systems.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA