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1.
Cancer Lett ; 468: 27-40, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31604115

RESUMO

Patients with recurrent nasopharyngeal carcinoma (NPC) have more co-existing distant metastasis than those of no-recurrence and are more likely to suffer distant metastasis after re-irradiation than patients with newly diagnosed NPC. However, the relationship between radioresistance and distant metastasis and the mechanisms involved in radioresistance-associated metastasis are still unclear. In this study, we proved that C-C motif chemokine ligand 2 (CCL2) expression was significantly elevated in HONE1-IR cells and recurrent NPC tumour. Inhibition of CCL2 enhanced sensitivity to radiotherapy in NPC cells. Moreover, autocrine CCL2 promoted NPC cell adaptive radioresistance, metastasis and epithelial-mesenchymal transition. Additionally, p53 activated CCL2 transcription. High CCL2 expression was highly associated with poorer locoregional recurrence free survival, progression free survival and overall survival in patients with newly diagnosed NPC. Notably, high CCL2 expression was an independent prognostic factor for distant metastasis free survival in recurrent NPC patients. Our results provide insights into the autocrine signalling mechanisms of CCL2 and suggest that inhibition of autocrine CCL2 may be a candidate treatment strategy for management of radioresistant NPC.

2.
Chemosphere ; 239: 124833, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31526990

RESUMO

A facile and effective method has been developed to prepare hierarchical porous carbon nanofibers (PCNFs), carbon nanofibers supported nickel nanoparticles (PCNFs-Ni) and carbon nanofibers encapsulating gold nanoparticles (PCNFs-Au). PCNFs or PCNFs-Au were obtained by embedding metal-organic frameworks (e.g. ZIF-8 or ZIF-8-Au) into polyacrylonitrile via electrospinning and subsequent carbonization. In addition, PCNFs-Ni were obtained by impregnating PAN/ZIF-8 nanofibers in Ni(NO3)2·6H2O followed by carbonization. Both PCNF and PCNF-Ni exhibited excellent adsorption activities for methylene blue (MB) and congo red (CR). Especially, PCNF-Ni could be removed and separated via a magnet. PCNFs-Au showed excellent catalytic properties in the reduction reaction of 4-nitrophenol (4-NP).

3.
J Nanosci Nanotechnol ; 20(2): 789-801, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383074

RESUMO

Magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were prepared via a solution combustion process, and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), Energy dispersive X-ray Spectroscopy (EDX) and vibrating sample magnetometer (VSM). The magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites were employed to remove Congo red (CR) from aqueous solution, and the adsorption process was optimized by response surface methodology (RSM). The optimum conditions were the silica content of 12.6 wt%, the calcination temperature of 501 °C and the pH value of 7.13. The adsorption kinetics and the adsorption isotherm of CR onto magnetic Ni0.5Cu0.5Fe2O4/SiO2 nanocomposites at room temperature were investigated, and the intraparticle diffusion kinetics model and Redlich-Peterson isotherm model fitted well the respective process.

4.
Food Chem ; 306: 125616, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31622832

RESUMO

This research aimed to explore the role of protein S-nitrosylation in regulating the tenderness of postmortem beef, from the perspective of µ-calpain autolysis and protein proteolysis. Five bovine semimembranosus muscles were incubated with three treatments including S-nitrosoglutathione (GSNO, nitric oxide donor), normal saline and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME, nitric oxide synthase inhibitor). The results showed that the level of protein S-nitrosylation was improved by GSNO treatment and reduced by L-NAME treatment (p < 0.05). Compared to the control, GSNO treatment had higher shear force while L-NAME treatment presented lower shear force at 7 d postmortem (p < 0.05). In addition, µ-calpain autolysis, myofibrillar protein and desmin degradation were reduced by GSNO treatment and accelerated by L-NAME treatment (p < 0.05). Therefore, it can be speculated that protein S-nitrosylation could affect beef tenderization by regulating the autolysis of µ-calpain and the degradation of myofibrillar proteins.

5.
Food Chem ; 307: 125645, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31644983

RESUMO

A simple electrochemical sensor was developed to determine the concentration of Ca2+ in meat. Graphene was treated with oxygen plasma for 10 s and 30 s comparing with the pristine graphene. Through analyzing morphology and chemical composition, the graphene with the lowest defect density was chosen to mix with bovine serum albumin molecule-functionalized gold nanoparticles. It was interesting that only a few gold nanoparticles were trapped in the graphene with 10 s plasma treatment. Then, under the optimal condition measured, the limit of detection was detected as 3.9 × 10-8 M with a linear relationship from 5 × 10-8 to 3 × 10-4 M. Finally, the proposed electrochemical method was applied to detect Ca2+ in the pork sample with stability and reproducibility verified by parallel detections. Thus, the proposed method demonstrates its potential for effectively detecting Ca2+ in meat and prominently reduces time consumption on operations and pretreatments.

6.
J Sci Food Agric ; 100(1): 258-267, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31512250

RESUMO

BACKGROUND: Composite gels were individually prepared from 20 g kg-1 myofibrillar protein (MP) imbedded with typical native starch (potato, tapioca, rice or corn starch) in 0.6 mol L-1 NaCl at pH 6.2. The gel strength, water holding capacity, rheological properties and microstructure of the obtained myofibrillar protein-starch composite gels were evaluated. RESULTS: Tapioca starch improved (P < 0.05) gel strength and water holding capacity of MP composite gel at 80 °C. Rheological properties of MP-starch composites differed significantly with the addition of different types of native starch. Additionally, the promoting effect of starch on the storage modulus of the composite gels positively correlated with the gelatinization properties of different typical starch. Environmental scanning electron microscopy showed that the filling effect of starch on the composite gel was related to the pasting temperature and particle size of typical starch, with almost no particles forming at 80 °C. Moreover, the addition of starch changed the relaxation peak area and increased the relaxation time in nuclear magnetic resonance tests, which suggested that starch could improve the water holding capacity of MP-starch composite gels. CONCLUSION: Different typical native starch has varied impacts on the gel strength, water holding capacity, rheological properties and microstructure of MP gels, indicating the potential and feasibility of these typical native starches as an addition agent to modify the textural properties in comminuted meat products. © 2019 Society of Chemical Industry.

7.
Sci Total Environ ; 701: 134776, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31726411

RESUMO

As an important part of groundwater systems, aquitards may have a considerable impact on the quality of groundwater in an aquifer. Organic carbon (OC) serves as an important component in biogeochemical processes which affects the hydrochemical composition of pore water; the association of OC with (Iron) Fe-containing minerals is recognized as an important stabilization mechanism for OC. However, the characteristics of the process forming complexes between OC and Fe oxides in subsurface aquitards and the contribution of OC to aquifers has been poorly understood. In this study, the content, speciation and reaction types of OC and Fe oxides were investigated to reveal the characteristics and mechanisms of OC-Fe interaction in subsurface aquitards at different depths in two typical sedimentary facies of the Jianghan Plain in central China. The total organic carbon (TOC) contents of aquitard sediments in alluvial-lacustrine and residual slope facies were 7.25 ±â€¯5.10 and 2.79 ±â€¯0.86 mg g-1, respectively. In general, the proportion of the heavy fraction of OC (HFOC) to TOC gradually increased with increasing depth because consumption of the light fraction of OC (LFOC) that caused more HFOC to remain in sediment. On average, Fe bound-OC contributed 30.3% and 31.6% of TOC by adsorption and coprecipitation, respectively. The adsorption-stabilized OC has not changed obviously but the coprecipitation-stabilized OC increased gradually with depth. Coprecipitation stabilized more OC in a stable environment (residual slope facies) when compared with an unstable environment (alluvial-lacustrine facies), which can be supported by the greater average ratio of FePP bound-OC:TOC ratio and increased enrichment of carboxylates and aromatics in a stable environment. The transformation of OC-Fe complexes could affect the transport of As, Cr and ammonium which chemically bind to organic matter (OM) and Fe minerals from sediment to pore water by reductive dissolution. The findings of this study are helpful in understanding the interaction between OC and Fe oxides in subsurface aquitard environments, in which reaction products may affect adjacent aquifers.

8.
J Nanosci Nanotechnol ; 20(5): 2675-2688, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635602

RESUMO

Led to significant capacity improvement to 1800 mA/h after 100 cycles for nano-graphene-N4, which is the first report for a carbonaceous materials anode. In addition, the doping level, id est, number of nitrogen atoms, had a significant influence on the molecular self-assembled structures through hierarchical self-assembly. As the nitrogen concentration increased, the d-space between the nanosheets increased from 3.4 to 4.3. The capacity of the nano-graphene increased greatly from 500 mAh/g for nano-graphene without N-doping to 1800 mAh/g for nano-graphene with nanographene-N4, indicating that the capacity is related to the structures, and was defined and the relationship between performance and structure was determined.

9.
J Nanosci Nanotechnol ; 20(5): 3031-3037, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635644

RESUMO

The α-Fe2O3 nanoparticles were prepared via the co-precipitation process, and they were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). The effect of the water bath temperature on the average grain size of the α-Fe2O3 nanoparticles was investigated. The minimum grain size of the α-Fe2O3 nanoparticles was 19.6 nm when the water bath temperature was 40 °C. Furthermore, the α-Fe2O3 nanoparticles were successfully modified with silica (SiO2) and chitosan (CTS) using the idea of nanoarchitectonics. the experimental results showed that, the average diameter of the as-prepared α-Fe2O3/SiO2 nanocomposites was around 65 nm; while, the average hydrodynamic diameter of the α-Fe2O3/CTS nanocomposites increased gradually with the increase of chitosan in solution. When the mass ratio of chitosan and the α-Fe2O3 nanoparticles reached 40:1, the diameter distribution range of the α-Fe2O3/CTS nanocomposites was very wide of 100- 900 nm, so the mass ratio of chitosan and the α-Fe2O3 nanoparticles was selected from 10:1 to 20:1 to be applied.

10.
J Nanosci Nanotechnol ; 20(4): 2330-2336, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492244

RESUMO

We report a facile synthesis of Au nanoparticles (Au NPs) decorated Fe3O4@carbon (Fe3O4@C) core-shell nanoparticles (denoted as Fe3O4@C@Au) with easy inverting the surface charge of the composites through the hexadecyl trimethyl ammonium bromide (CTAB) treatment (denoted as Fe3O4@C@Au-CTAB). Nanoparticles of both kinds have been studied as catalysts for an anionic methyl orange (MO) reduction. Fe3O4@C@Au-CTAB positively charged exhibits a faster catalytic ability toward anionic MO than negatively charged Fe3O4@C@Au, indicating an effect of the electrostatic interaction on catalytic reduction. The facile and green preparation, high catalytic capability, selective surface property and recyclability make Fe3O4@C@Au-CTAB an appropriate platform for nanocatalysis in wastewater treatment.

11.
Org Lett ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31794234

RESUMO

Regioselectivity-switchable reactions hold irreplaceable importance in the construction of diversified architectures. In this work, Brønsted base-catalyzed regioselectivity-switchable annulations between alkynyl α-diketones and α-cyanoketones have been achieved for the first time, delivering a series of skeletally thoroughly different dihydrofurofuran and furan derivatives. A range of novel transformations of the products can be realized. The work also demonstrates the unique features of alkynyl α-diketone chemistry, which are in sharp contrast to the current understanding of ynone-related chemistry.

12.
Theranostics ; 9(25): 7648-7665, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31695792

RESUMO

Alternative splicing (AS) has emerged as a key event in tumor development and microenvironment formation. However, comprehensive analysis of AS and its clinical significance in head and neck squamous cell carcinoma (HNSC) is urgently required. Methods: Genome-wide profiling of AS events using RNA-Seq data from The Cancer Genome Atlas (TCGA) program was performed in a cohort of 464 patients with HNSC. Cancer-associated AS events (CASEs) were identified between paired HNSC and adjacent normal tissues and evaluated in functional enrichment analysis. Splicing networks and prognostic models were constructed using bioinformatics tools. Unsupervised clustering of the CASEs identified was conducted and associations with clinical, molecular and immune features were analyzed. Results: We detected a total of 32,309 AS events and identified 473 CASEs in HNSC; among these, 91 were validated in an independent cohort (n = 15). Functional protein domains were frequently altered, especially by CASEs affecting cancer drivers, such as PCSK5. CASE parent genes were significantly enriched in pathways related to HNSC and the tumor immune microenvironment, such as the viral carcinogenesis (FDR < 0.001), Human Papillomavirus infection (FDR < 0.001), chemokine (FDR < 0.001) and T cell receptor (FDR < 0.001) signaling pathways. CASEs enriched in immune-related pathways were closely associated with immune cell infiltration and cytolytic activity. AS regulatory networks suggested a significant association between splicing factor (SF) expression and CASEs and might be regulated by SF methylation. Eighteen CASEs were identified as independent prognostic factors for overall and disease-free survival. Unsupervised clustering analysis revealed distinct correlations between AS-based clusters and prognosis, molecular characteristics and immune features. Immunogenic features and immune subgroups cooperatively depict the immune features of AS-based clusters. Conclusion: This comprehensive genome-wide analysis of the AS landscape in HNSC revealed novel AS events related to carcinogenesis and immune microenvironment, with implications for prognosis and therapeutic responses.

13.
Food Funct ; 10(11): 7576-7587, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31687710

RESUMO

Honey is a natural sweetener that contains a large amount of monosaccharides such as glucose and fructose, as well as small amounts of disaccharides and trisaccharides such as sucrose and pine trisaccharides. In addition to carbohydrates, honey also contains vitamins, minerals, enzymes, amino acids, and polyphenols including phenolic acids and flavonoids. The polyphenols in honey have been proved to have great antioxidant activity, besides inhibiting α-glycosidase activity and improving blood-lipid metabolism. However, whether it is safe for diabetic patients to consume honey remains controversial. This study investigated the effects of honey, metformin and their combination on the characteristic pathological changes and glucose metabolism in STZ-induced diabetic mice over five weeks. Our results showed that honey and its combination with metformin could prevent hyperglycemia, stimulate insulin secretion, reduce liver fat accumulation, attenuate liver injury and kidney damage in STZ-induced diabetic mice. Moreover, treatment with honey or combination of honey and metformin significantly enhanced glucokinase (GK) activity (p < 0.05), and meanwhile suppressed the activities of glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate carboxylase (PC) and pyruvate dehydrogenase kinases (PDK) (p < 0.05) in diabetic mice.

14.
Bull Environ Contam Toxicol ; 103(6): 834-840, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31676938

RESUMO

Sugarcane is a potential species for use in heavy metal remediation. To analyze the effect of excess copper on sugarcane, the biomass, mineral nutrient content and activities of antioxidative enzymes were measured under copper stress. The results revealed that the biomass of roots and shoots significantly decreased with increasing copper concentration in solution. Most copper accumulated in the roots, and the translocation factor of copper decreased with an increase in copper stress. The MDA content in sugarcane roots notably increased under copper stress. The POD activity in sugarcane roots increased, and CAT activity decreased under copper stress. The Zn, Fe and Mn contents in shoots increased significantly under 200 µmol L-1 Cu2+ treatments. The Zn and Mg contents in roots notably decreased under copper stress, while the Zn and Mg translocation factors increased. These results indicated that the increase in POD activity and the modification of mineral nutrient uptake and transfer might play an important role in reducing the detrimental effects of excess copper.

15.
J Ethnopharmacol ; : 112365, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31678414

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ginkgo biloba L. (Ginkgoaceae) is a traditional Chinese medicine known to treating stroke and other cardio-cerebrovascular diseases for thousands of years in China. Ginkgo diterpene lactones (GDL) attracted much attention because of their neuroprotective properties. AIM OF THE STUDY: To uncover the effects of GDL, which consist of ginkgolide A (GA), ginkgolide B (GB), and ginkgolide K (GK), on ischemic stroke, as well as the underlying molecular mechanisms. MATERIALS AND METHODS: We used middle cerebral artery occlusion/reperfusion (MCAO/R) and oxygen-glucose deprivation/reoxygenation (OGD/R) models mimicking the process of ischemia/reperfusion in vivo and in vitro, respectively. Anticoagulant effects of GDL were investigated on platelet activating factor (PAF), arachidonic acid (AA) and adenosine diphosphate (ADP)-induced platelet aggregation both in vivo and in vitro. We also evaluated the effects of GDL on lipopolysaccharide (LPS)-induced inflammatory response in primary cultured rats' astrocytes. Infarct size, neurological deficit score, and brain edema were measured at 72 h after MCAO. Immunohistochemistry was utilized to analyze neurons necrosis and astrocytes activation. Expression of pro-inflammatory cytokines, including tumor necrotic factor-α (TNF-α) and interleukin-1ß (IL-1ß) were detected using enzyme-linked immunosorbent assay (ELISA) and real time PCR. The levels of toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) were assessed by real time PCR or Western blot. RESULTS: Compared with MCAO/R rats, GDL significantly reduced infarct size and brain edema, improved neurological deficit score. Meanwhile, GDL suppressed platelet aggregation, astrocytes activation, pro-inflammatory cytokines releasing, TLR4 mRNA expression and transfer of NF-κB from cytoplasm to nucleus. Furthermore, GDL alleviated OGD/R injury and LPS-induced inflammatory response in primary astrocytes, characterized by promoting cell viability, decreasing lactate dehydrogenase (LDH) activity, and inhibiting IL-1ß and TNF-α releasing. CONCLUSIONS: In summary, GDL attenuate cerebral ischemic injury, inhibit platelet aggregation and astrocytes activation. The anti-inflammatory activity might be associated with the downregulation of TLR4/NF-κB signal pathway. Our present findings provide an innovative insight into the novel treatment of GDL in ischemic stroke therapy.

16.
Plant Biotechnol J ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31693306

RESUMO

Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is a devastating disease of rapeseed (Brassica napus L.). To date, the genetic mechanisms of rapeseed' interactions with S. sclerotiorum are not fully understood, and molecular-based breeding is still the most effective control strategy for this disease. Here, Arabidopsis thaliana GDSL1 was characterized as an extracellular GDSL lipase gene functioning in Sclerotinia resistance. Loss of AtGDSL1 function resulted in enhanced susceptibility to S. sclerotiorum. Conversely, overexpression of AtGDSL1 in B. napus enhanced resistance, which was associated with increased reactive oxygen species (ROS) and salicylic acid (SA) levels, and reduced jasmonic acid levels. In addition, AtGDSL1 can cause an increase in lipid precursor phosphatidic acid levels, which may lead to the activation of downstream ROS/SA defence-related pathways. However, the rapeseed BnGDSL1 with highest sequence similarity to AtGDSL1 had no effect on SSR resistance. A candidate gene association study revealed that only one AtGDSL1 homolog from rapeseed, BnaC07g35650D (BnGLIP1), significantly contributed to resistance traits in a natural B. napus population, and the resistance function was also confirmed by a transient expression assay in tobacco leaves. Moreover, genomic analyses revealed that BnGLIP1 locus was embedded in a selected region associated with SSR resistance during the breeding process, and its elite allele type belonged to a minor allele in the population. Thus, BnGLIP1 is the functional equivalent of AtGDSL1 and has a broad application in rapeseed S. sclerotiorum-resistance breeding.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31740131

RESUMO

As one type of bifunctional oxygen electrocatalyst for Zn-air battery, herein, FeNi alloy was successfully embedded into N-doped carbon with tailored architectures by integrating MOF precursor method and polymer coating/encapsulation strategy. The content of Fe in primary precursor has been proven to be able to obviously affect the morphology of the final catalyst. Benefiting from the mature active site (e.g. FeNi alloy) and the stable carbon matrix, a series of catalysts exhibited good performance towards ORR and OER. Of great significance, a particular ratio of Fe/Ni happened to be able to catalyze the growth of 1D bamboo-like carbon nanotubes, giving rise to a conductive network to diffuse ORR/OER-relevant species. Apparently, a low discharge-charge voltage gap (1.1 V) was acquired in a liquid Zn-air battery with 1.5FeNi@NCNT air cathode. Moreover, the solid-state Zn-air battery assembled on it also displayed a high open circuit voltage (1.38 V) and yielded a high power density of 81 mW cm-2 at 0.83 V. This would leverage a choice to tailor carbon geometry of FeNi alloy-based active sites for ORR/OER and further serve for devices of practical significance.

18.
Phytother Res ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31667930

RESUMO

Mas-related G protein-coupled receptor-X2 (MRGPRX2) expressed on mast cells (MCs) has been shown to be a pivotal target for pseudo-allergic diseases. Therefore, MRGPRX2 might be a therapeutic target for allergic contact dermatitis, atopic dermatitis, and red man syndrome. Paeoniflorin (PF) was reported to have an antiinflammatory effect in neuroinflammation, enteritis, and so forth. In this study, we investigated the anti-pseudo-allergic effect of PF and the underlying molecular mechanisms. Our results showed that PF can suppress compound 48/80 (C48/80)-induced PCA and MCs degranulation in vivo, in a dose-dependent manner. Moreover, PF can reduce C48/80-induced calcium influx and suppress MC degranulation and chemokines release in vitro. PF can downregulate the phosphorylation levels of key kinases in PLCγ-regulated calcium influx and subsequent cytokine synthesis pathways. Our study revealed that PF could inhibit C48/80-induced allergic responses both in vivo and in vitro. As such, it may be regarded as a novel inhibitor for preventing MRGPRX2-mediated allergic diseases.

19.
Anal Chim Acta ; 1092: 32-41, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31708030

RESUMO

Deer-hide gelatin (DHG) is an important animal-derived traditional Chinese medicine (TCM), which has been applied in TCM for over 400 years. However, it is extremely difficult to distinguish DHG with adulteration or made with other animal skins due to the highly processing procedure. Therefore, a simple strategy for identifying species-specific peptide biomarkers in deer-hide gelatin (DHG) is needed. In the present study, untargeted and targeted mass spectrometry approaches were implemented to analyze comprehensive peptidomic profiles of trypsin-digested animal gelatins. Mathematics set theory was then used to interrogate the relationship between different samples and peptides in the target species set, while the peptides were not considered as species-specific biomarkers in other sets. Two peptides were identified as DHG-specific peptides. Targeted mass spectrometry approach was then used to verify these two peptides. It showed that these two peptides could be used for distinguishing DHG from other animal hide gelatins. The present strategy provides a simple method for peptide biomarker discovery, which can be applied in the identification of specific peptides in some highly processed animal derived traditional Chinese medicines (TCMs). Thus, the present work provides an effective strategy for rapid, simple discovery and application of species-specific peptide biomarkers to ensure animal derived TCMs quality and make them authenticable and traceable.

20.
J Ethnopharmacol ; : 112412, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31751649

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Xiaoshui decoction (XSD) is a traditional Chinese medicine compound prescription that has been shown to reinforce the spleen and remove the fluid retention, while being widely used in the treatment of malignant pleural effusion (MPE). We previously reported that XSD alleviates symptoms and improves the quality of life in patients with MPE; however, the mechanism employed by XSD on MPE has not yet been elucidated. AIM OF THE STUDY: To investigate the role and mechanism of XSD in inhibiting the development of MPE, and in regulating macrophage polarization in vitro and in vivo. MATERIALS AND METHODS: A murine MPE model was used to study the effect of XSD on MPE. Mice with MPE were randomly allocated to a control group and XSD-low-dose (1.144 g/mL), XSD-middle-dose (2.288 g/mL), XSD-high-dose (4.576 g/mL), or cisplatin groups. RAW264.7 cells were induced to form tumor-associated macrophages (TAMs) as well as M1 and M2 macrophages using different conditioned media in vitro. RESULTS: XSD effectively inhibited MPE formation, reduced pleural permeability and angiogenesis, and prolonged mice survival. Particularly, XSD treatment induced the polarization of TAMs to the M1 phenotype in MPE. Moreover, in-vitro XSD remarkably promoted the expansion of M1 macrophages and reduced M2 macrophages by enhancing autophagy. CONCLUSIONS: XSD inhibits MPE development and regulates macrophage polarization by activating autophagy, indicating that XSD may serve as a novel option for integrative MPE therapies.

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