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1.
J Cell Mol Med ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960591

RESUMO

Blockade of cell cycle re-entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture (Coptis chinensis, Pinellia ternata and Fructus trichosanthis) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re-entry in quiescent lung cancer cells by reduction of FACT and c-MYC mRNA and protein levels, with concomitant decrease in H3K4 tri-methylation and RNA polymerase II occupancy at FACT and c-MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c-MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.

2.
Trop Med Int Health ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31912627

RESUMO

OBJECTIVE: To evaluate the association between oral third-generation cephalosporin antibiotic treatment and mortality in Ebola virus disease (EVD). METHODS: This retrospective cohort studied EVD-infected patients admitted to five Ebola Treatment Units in Sierra Leone and Liberia during 2014-15. Empiric treatment with cefixime 400 mg once daily for five days was the clinical protocol; however, due to resource variability, only a subset of patients received treatment. Data on sociodemographics, clinical characteristics, malaria status and Ebola viral loads were collected. The primary outcome was mortality compared between cases treated with cefixime within 48 h of admission to those not treated within 48 h. Propensity scores were derived using clinical covariates. Mortality between treated and untreated cases was compared using propensity-matched conditional logistic regression and bootstrapped log-linear regression analyses to calculate an odds ratio (OR) and relative risk (RR), respectively, with associated 95% confidence intervals (CI). RESULTS: Of 424 cases analysed, 360 (84.9%) met the cefixime treatment definition. The mean age was 30.5 years and 40.3% were male. Median cefixime treatment duration was 4 days (IQR: 3, 5). Among cefixime-treated patients, mortality was 54.7% (95% CI: 49.6-59.8%) vs. 73.4% (95% CI: 61.5-82.7%) in untreated patients. In conditional logistic regression, mortality likelihood was significantly lower among cases receiving cefixime (OR = 0.48, 95% CI: 0.32-0.71; P = 0.01). In the bootstrap analysis, a non-significant risk reduction was found with cefixime treatment (RR = 0.82, 95% CI: 0.64-1.16, P = 0.11). CONCLUSION: Early oral cefixime may be associated with reduced mortality in EVD and warrants further investigation.

3.
Cell Death Dis ; 11(1): 41, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31959918

RESUMO

Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer. Despite recently improved management of LSCC, chemotherapy resistance of patients remains a challenge. In this study, we identified that long noncoding RNA FOXD2-AS1 regulates LSCC therapeutic resistance by augmenting LSCC stemness. LSCC chemotherapy-resistant patients showed increased FOXD2-AS1 expression compared with that in chemotherapy-sensitive patients, which predicted poor prognosis. Gain- or loss-of-function experiments showed that upregulated FOXD2-AS1 maintained cancer stemness, reducing the response to chemotherapy, while FOXD2-AS1 downregulation had the opposite effects. FOXD2-AS1 acted as a scaffold for STAT3 and PRMT5, promoting STAT3 transcriptional activity, which is essential to maintain cancer stemness and promote chemotherapeutic resistance. Interfering with FOXD2-AS1 using short hairpin RNA rescued LSCC's chemotherapeutic sensitivity. Thus, FOXD2-AS1 promotes LSCC chemotherapeutic resistance and is an upstream activator of STAT3, making FOXD2-AS1 a potential therapeutic target to improve the chemotherapy effect in LSCC patients.

4.
Sci Total Environ ; 713: 136357, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31962241

RESUMO

Microplastic (MP) ingestion has been recorded in 39 zooplankton species from 28 taxonomic orders, and marine zooplankton are the organisms most susceptible to MPs. However, few field studies have examined the characteristics and major influencing factors of MPs in marine zooplankton. The present study investigated the shape, color, size, chemical composition and quantity of MPs in zooplankton in the rainy and dry seasons in the Bohai Sea. Furthermore, the relationship between the MPs in zooplankton and the MPs in seawater was studied. The results showed that the MPs in zooplankton of the Bohai Sea were dominated by blue fibers. In the rainy and dry seasons, fibers accounted for 92% and 93%, respectively, of all ingested MPs, while 50% and 55%, respectively, of ingested MPs were blue. The average size of MPs in zooplankton was 1300 ± 1520 µm in the rainy season and 1040 ± 1060 µm in the dry season. Regarding the MP chemical composition, in the rainy and dry seasons, the ingested MPs were dominated by cellophane, which accounted for 53% and 68%, respectively, of MPs, followed by polyester (PET), which accounted for 18% and 20%, respectively, of MPs. The composition of MPs in zooplankton was mainly affected by the composition of MPs in seawater. No significant difference in the MP composition was observed between the two seasons or among the different zooplankton groups. The MP number was significantly higher in individual medusa than in individuals of other zooplankton groups. The mean quantity of MPs in the Bohai Sea zooplankton community in the rainy season was 2.03 ± 2.87 piece/m3, which was significantly higher than that in the dry season, 0.41 ± 0.38 piece/m3. The above results will provide a reference for marine ecological risk assessments based on the characteristics of MPs in natural seas.

5.
Sci Total Environ ; 710: 136403, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31927294

RESUMO

Considering its ubiquitous occurrence and potential adverse effects of organophosphorus flame retardant (OPFR), it is urgent to explore the efficient treatment for OPFRs wastewater. Thus, integrated vertical-flow constructed wetlands (IVCWs) were set up to comparatively evaluate their nitrogen removal capacity under tidal flow operations and to investigate environmental behavior and rhizosphere microbial responses after short-term exposure to three OPFRs. The results show that IVCWs have an excellent TN removal rate (628.13 ± 110.63 mg m-2 d-1) and moderate mitigation efficiencies (48.37 ± 9.52 to 82.28 ± 7.48%) for target OPFRs when treating low-C/N ratio wastewater. Moreover, the sorption of selected OPFRs to soil (28.85-308.41 ng g-1, dry weight (dw)), igneous rock (659.85-970.80 ng g-1 dw) and zeolite (1045.60-1351.70 ng g-1 dw) and accumulation in tissues of C. alternifolius (0-289.68 ng g-1 dw) and P. australis (0.56-108.22 ng g-1 dw) showed a hydrophobicity-specific feature. Namely, the highly hydrophobic compound tricresyl phosphate (TCrP) partitioned preferentially to sediment, and the chlorinated analytes were more easily taken up and then translocated into the plant body. Simultaneously, further mass balance analysis revealed the fate of OPFRs in IVCW components. A total of 53.25% of the highly hydrophobic TCrP inflow mass settled in sediment, while tris (2-chloroethyl) phosphate (TCEP) and tris (1-chloro-2-propyl) phosphate (TCPP) were more liable to discharge (35.33-50.89%) and other pathways (38.77-39.87%). Furthermore, the abundance of aerobic denitrifying bacteria (AD) in rhizosphere soil (2.25-5.12%), jointly with the prevalence of nitrobacteria (NOBs, 1.84-13.60%) and denitrifying bacteria (DNBs, 5.84-7.89%) in sublayer matrices, was responsible for superior TN removal. Additionally, the rhizosphere microbial richness, diversity and nitrogen-related microorganisms were clearly influenced by the presence of OPFRs. Notably, the genera Pseudomonas and Sphingobium might be the functional microorganisms for mixture OPFRs biodegradation.

6.
Medicine (Baltimore) ; 99(1): e18667, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895832

RESUMO

BACKGROUND: Laparoscopic right hepatectomy (LRH) is one of the most challenging procedures. Right liver resections have been always performed in open procedure and open right hepatectomy (ORH) was initially considered as routine way. Moreover, it is unclear how beneficial the minimally invasive technique is to patients; thus, we conducted a meta-analysis to acquire a more reliable conclusion about the feasibility and safety of LRH compared with ORH. METHODS: We comprehensively searched the electronic databases of PubMed, Embase, and the Cochrane Library using the key words. Meta-analysis was performed using the Review Manager, with results expressed as odds ratio and weighted mean difference with 95% confidence intervals. The fixed-effect model was selected initially if high heterogeneity was not present between the studies; otherwise, the randomized-effect model was used. Subgroup analysis was performed based on different surgical methods of pure laparoscopic operation or hand-assisted operation. RESULTS: Seven studies with 467 patients were included. In the overall analysis, less intraoperative blood loss (MD = -155.17; 95% CI, -238.89, -71.45; P = .0003) and a shorter length of stay (MD = -4.45; 95% CI, -5.84, -3.07; P < .00001) were observed in the LRH group compared to the ORH group. There were fewer overall complications (OR = 0.30; 95% CI, 0.10, 0.90; P = 0.03) and severe complications (OR = 0.24; 95% CI, 0.10, 0.58; P = .002;) in the LRH group than in the ORH group. The disadvantage of LRH was the longer operative time (MD = 49.39; 95% CI, 5.33, 93.45; P = .03). No significant difference was observed between the 2 groups in portal occlusion, rate of R0 resection, transfusion rate, mild complications, and postoperative mortality. In the subgroup analysis, intraoperative blood loss was significantly lower in the pure LRH group and hand-assist LRH group compared with ORH group. Length of stay was shorter by use of pure LRH and hand-assisted LRH manners than ORH. The incidence rate of complications was lower in the pure LRH group than in the ORH group. In contrast, there was no significant difference between hand-assisted LRH group and ORH group. CONCLUSION: Compared to ORH, LRH has short-term surgical advantages and leads to a shorter recovery time in selected patients. We speculate that the operative time of LRH is closer with ORH. Overall, LRH can be considered a feasible choice in routine clinical practice with experienced surgeons, although more evidence is needed to make a definitive conclusion.


Assuntos
Hepatectomia/métodos , Neoplasias Hepáticas/cirurgia , Humanos , Laparoscopia , Complicações Pós-Operatórias
7.
J Phys Chem Lett ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31931563

RESUMO

It is widely accepted that the sensitivity of Surface-enhanced Raman Spectroscopy (SERS) is mainly manipulated by electromagnetic enhancement mechanism (EM). Herein, we figured out that the direct adsorption of the target on the SERS active surface is vital as well, through the systematic investigation of the SERS behavior of three positively charged molecules on negatively charged gold (Au) or silver nanoparticles (Ag NPs). Facilitated by the synergistic effect among the molecule, the surface, and the specific adsorbed halide ions (Cl, Br, and I), high SERS sensitivity for trace target was realized, which was mainly from the directly adsorbed molecules. Noteworthy, little contribution from the non-directly adsorbed molecules was discernable, although the EM enhancement was in the same level for these two surface species dwelling within a distance significantly less than 1 nm from the surface. Further, the related strategy for trace detection sheds light on how to realize sensitive SERS detection of new targets.

8.
DNA Cell Biol ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31934791

RESUMO

Lung cancer with highest morbidity and mortality seriously threatens human health worldwide. Long noncoding RNAs (lncRNAs) exert important biological functions by acting as microRNA, which is implicated in tumorigenesis and cancer development. Previous work has reported that lncRNA-ATB expression was significantly upregulated in lung adenocarcinoma tissues and promoted tumor progression; however, the mechanisms of lncRNA-ATB in lung squamous carcinoma (LSC) are still fairly elusive. In our study, lncRNA-ATB expression also markedly increases in LSC tissues and cell lines in comparison to the adjacent normal tissues and normal lung epithelial cells, respectively. Functional experiments indicate that lncRNA-ATB overexpression improves the proliferative, migratory, and invasive capabilities of normal lung epithelial cells compared with control group. Furthermore, the migratory and invasive abilities are strikingly inhibited in lncRNA-ATB silenced LSC cells. Mechanistically, lncRNA-ATB directly binds to microRNA-590-5p and downregulates microRNA-590-5p level, leading to the upregulation of NF-90 expression. In addition, lncRNA-ATB overexpression promotes the epithelial-mesenchymal transition process, where lncRNA-ATB overexpression facilitates the expression of mesenchymal phenotype related molecules N-cadherin and vimentin, while restrains the expression of epithelial phenotype related proteins E-cadherin and CK-19, compared to the control. Conversely, microRNA-590-5p mimics can reverse the results caused by lncRNA-ATB overexpression. Taken together, our initial data suggest that lncRNA-ATB overexpression may promote the progression of LSC by modulating the microRNA-590-5p/NF-90 axis.

9.
Environ Health Perspect ; 128(1): 17006, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31909654

RESUMO

BACKGROUND: Both extreme heat and air pollution exposure during pregnancy have been associated with preterm birth; however, their combined effects are unclear. OBJECTIVES: Our goal was to estimate the independent and joint effects of heatwaves and fine particulate matter [PM <2.5µm in aerodynamic diameter (PM2.5)], exposure during the final gestational week on preterm birth. METHODS: Using birth registry data from Guangzhou, China, we included 215,059 singleton live births in the warm season (1 May-31 October) between January 2015 and July 2017. Daily meteorological variables from 5 monitoring stations and PM2.5 concentrations from 11 sites were used to estimate district-specific exposures. A series of cut off temperature thresholds and durations (2, 3, and 4 consecutive d) were used to define 15 different heatwaves. Cox proportional hazard models were used to estimate the effects of heatwaves and PM2.5 exposures during the final week on preterm birth, and departures from additive joint effects were assessed using the relative excess risk due to interaction (RERI). RESULTS: Numbers of preterm births increased in association with heatwave exposures during the final gestational week. Depending on the heatwave definition used, hazard ratios (HRs) ranged from 1.10 (95% CI: 1.01, 1.20) to 1.92 (1.39, 2.64). Associations were stronger for more intense heatwaves. Combined effects of PM2.5 exposures and heatwaves appeared to be synergistic (RERIs>0) for less extreme heatwaves (i.e., shorter or with relatively low temperature thresholds) but were less than additive (RERIs<0) for more intense heatwaves. CONCLUSIONS: Our research strengthens the evidence that exposure to heatwaves during the final gestational week can independently trigger preterm birth. Moderate heatwaves may also act synergistically with PM2.5 exposure to increase risk of preterm birth, which adds new evidence to the current understanding of combined effects of air pollution and meteorological variables on adverse birth outcomes. https://doi.org/10.1289/EHP5117.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31912458

RESUMO

Lentiviral expression vectors for calcitonin gene-related peptide (CGRP) were used to transfect rat bone marrow mesenchymal stem cells (MSCs). After assessing the biological characteristics of proliferation and aging in MSCs transfected with CGRP, we observed the effects of the CGRP-modified rat MSCs on the migration and proliferation of rat vascular smooth muscle cells (VSMCs) in vitro. Rat MSCs were isolated, cultured in vitro, and identified by flow cytometry. A CGRP recombinant lentivirus was transfected into MSCs. The transfection efficiency was determined by fluorescence microscopy and flow cytometry, and CGRP in MSCs was detected by real-time quantitative PCR, ELISA, and immunofluorescence. The proliferation and senescence of CGRP-modified MSCs were evaluated by MTT assay and beta-galactosidase staining. VSMCs were isolated, cultured in vitro, and identified by immunofluorescence. CGRP-modified MSCs and VSMCs were cocultured in a Transwell system. The proliferation and migration of VSMCs were evaluated by scratch testing and the MTT method. Rat bone marrow MSCs showed a spindle-shaped morphology, adherent growth in vitro, positive CD29 and CD90 expression, and negative CD45 expression. CGRP was stably expressed in MSCs after 48 h of recombinant lentivirus transfection. CGRP mRNA and protein secretion in CGRP recombinant lentivirus-transfected MSCs were higher than that in control MSCs. Immunofluorescence showed that CGRP protein could be expressed in CGRP-modified MSCs. The proliferation ability and senescence rates did not differ between lentivirus-transfected MSCs and untransfected MSCs. Rat VSMCs expressed α-SMA protein and exhibited a spindle-shaped morphology and adherent growth in vitro. In Transwell coculture experiments, scratch testing of VSMCs showed that CGRP-modified MSCs could reduce VSMC proliferation and migration. The CGRP gene can be stably expressed in MSCs after CGRP recombinant lentivirus transfection. CGRP recombinant lentivirus transfection has little effect on the proliferation or senescence of MSCs, and CGRP-modified MSCs can inhibit the proliferation and migration of VSMCs. These results lay a foundation for research on the use of CGRP gene-engineered MSCs in restenosis therapy.

11.
Chemosphere ; 247: 125831, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31935576

RESUMO

Moving-bed biofilm reactor (MBBR) is a well-established technology for simultaneous nitrification and denitrification (SND). In MBBR, biofilm development and pollutant removal performance are strictly governed by the physico-chemical properties of the carriers. In this study, novel surface-modified carriers with enhanced hydrophilicity (surface contact angle of 60.2 ± 2.3°) and positively-charged surfaces (+11.7 ± 1.1 mV, pH 7.0) had been prepared successfully via polymer blending, and they had also been implemented in SND system for the treatment of real domestic wastewater. Results showed that accelerated startup of SND with more biomass on the carriers was observed in MBBR system filled with surface-modified carriers. At low DO level (0.6-0.8 mg L-1) and low C/N ratio (≤5), highly efficient organics removal and SND performance could be achieved with COD removal, TN removal and SND efficiencies of 79.3-85.7%, 62.0-75.9% and 58.5-71.8%, respectively. The efficient performance of SND in MBBR system filled with surface-modified carriers was mainly attributed to the coexistence of enriched mixtrophic nitrifiers and denitrifiers like autotrophic nitrifers (Nitrosomonas, Nitrospira, Nitrobacter), heterotrophic nitrifers (Rudaea), aerobicdenitrifiers (Dokdonella, Terrimonas), anoxic denitrifiers (Gemmobacter, Ottowia, Methyloversatilis, Thermomonas) and N2O producer (Mesorhizobium).

12.
J Hazard Mater ; 389: 122116, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31972527

RESUMO

This study evaluated the effect of integrated bacterial culture and biochar on heavy metal (HM) stabilization and microbial activity during pig manure composting. High-throughput sequencing was carried out on six treatments, namely T1-T6, where T2 was single application of bacteria culture (C), T3 and T5 were supplemented with 12 % wood (WB) and wheat-straw biochar (WSB), respectively, and T4 and T6 had a combination of bacterial consortium mixed with biochar (12 % WB and 12 % WSB, respectively). T1 was used as control for the comparison. The results show that the populations of bacterial phyla were significantly greater in T6 and T4. The predominate phylum were Proteobacteria (56.22 %), Bacteroidetes (35.40 %), and Firmicutes (8.38 %), and the dominant genera were Marinimicrobium (53.14 %), Moheibacter (35.22 %), and Erysipelothrix (5.02 %). Additionally, the correlation analysis revealed the significance of T6, as the interaction of biochar and bacterial culture influenced the HM adsorption efficiency and microbial dynamics during composting. Overall, the integrated bacterial culture and biochar application promoted the immobilization of HMs (Cu and Zn) owing to improved adsorption, and enhanced the abundance and selectivity of the bacterial community to promote degradation and improving the safety and quality of the final compost product.

13.
Sleep Breath ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31898189

RESUMO

OBJECTIVE: The endocannabinoid system (ECS) regulates bone turn-over and remodeling. Chronic intermittent hypoxia (CIH) occurring during obstructive sleep apnea (OSA) may lead to disorders of the ECS and bone metabolism abnormalities. This study aimed to investigate whether or not the cannabinoid receptor 1 (CB1R) antagonist rimonabant (Ri) alleviates bone metabolism abnormalities and bone destruction induced by chronic intermittent hypoxia (CIH). METHODS: Healthy male Sprague Dawley (SD) rats (n=48) were randomly divided into 6 groups of 8 rats: 2 normal control (NC) groups, 2 intermittent hypoxia (IH) groups, and 2 IH + Ri groups. Rats in NC groups breathed room air for 4 weeks (4w NC group) and 6 weeks (6w NC group). Rats in IH groups experienced IH environment for 4 weeks (4w IH group) and 6 weeks (6w IH group). In addition to the same IH exposure, rats in IH + Ri group were given daily intraperitoneal injection of Ri at the dosage of 1.5 mg/kg/d for 4 weeks (4w IH + Ri group) and 6 weeks (6w IH + Ri group). Levels of serum tartrate-resistant acid phosphatase (TRAP, a marker of bone resorption) were determined by ELISA. Hematoxylin and eosin (HE) staining was performed on bone sections to observe the changes in bone microstructure. Expression of CB1R in bone tissue was determined by immunohistochemistry. RESULTS: TRAP levels were higher in the 4w IH and 6w IH groups than in the 4w NC and 6w NC groups; TRAP levels were lower in the 4w IH + Ri and 6w IH + Ri groups than in the 4w IH and 6w IH groups. HE staining showed that the morphology of bone cells in the NC group was normal, but the 4w IH group had mild edema of bone cells, reduction in trabecular bone, and destruction of bone microstructure. Changes were more severe in the 6w IH group than 4w IH. The 4w IH + Ri group was slightly improved compared with the 4w IH group. The 6w IH + Ri group was improved compared with the 4w IH + Ri group. The results of immunohistochemistry showed that the expression of CB1R in IH group was significantly higher than that in NC group. The expression of CB1R in the IH + Ri group was lower than that in the IH group. With the prolongation of hypoxia, the expression of CB1R in bone cells of IH group increased. The expression level of CB1R in IH + Ri group decreased with the prolongation of intervention time. Correlation analysis showed that the expression rate of CB1R in bone cells was positively correlated with the level of TRAP in serum. CONCLUSION: CIH increases serum TRAP levels and triggers metabolic bone disorder by activating bone CB1R. Intervention with CB1R antagonist (rimonabant) reduces the bone dysmetabolism in the CIH rat model.

14.
Chin Med J (Engl) ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31904729

RESUMO

BACKGROUND: Baicalein has been shown to have anti-inflammatory and anti-tumor activities. However, the mechanisms underlying its anti-inflammatory effect on colitis remain unclear. METHODS: A dextran sodium sulfate (DSS)-induced model of acute colitis was established in BALB/c mice (6-8 weeks old, weighing 18-22 g). Six groups of mice received: (1) water for 10 days (control), n = 6; (2) DSS 4% solution in the drinking water for 7 days, followed by normal water for 3 days, n = 7; (3), (4), and (5) as for group 2 plus baicalein (10, 20, 40 mg/kg) administered once daily starting on day 1, n = 6; and (6) as for (2) plus 5-aminosalicylic acid (50 mg/kg) administered once daily starting on day 1, n = 6. Body weights, stool consistency, and hematochezia were recorded, and the severity of colitis was evaluated using a disease activity index. On day 11, the mice were euthanized, and organs and blood were collected for analysis. Serum inflammatory factors were detected by enzyme-linked immunosorbent assay; CD11b-positive cells were analyzed by immunofluorescence microscopy; expression of retinoic-acid-receptor-related orphan nuclear receptor gamma, sphingosine kinase 1 (SPHK1), and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) was detected by immunohistochemistry; and expression of nucleotide-binding oligomerization domain 2 (NOD2), SPHK1, sphingosine 1-phosphate receptor 1 (S1PR1), total STAT3, and p-STAT3 were detected by western blotting analysis. Inter-group differences were compared using Student's t test. RESULTS: Baicalein treatment dose-dependently reduced DSS-induced weight loss (P < 0.01 or P < 0.05), splenomegaly (P < 0.01), and colonic damage, as reflected by amelioration of diarrhea, rectal bleeding, and colonic ulceration, congestion, edema (shown as colon length, P < 0.05 or P < 0.01), and inflammatory cell infiltration. Baicalein also significantly decreased the levels of inflammatory mediators in the serum (P < 0.01) and colon, and significantly inhibited expression of NOD2 SPHK1, S1PR1, and p-STAT3 in the colon (P < 0.05). CONCLUSIONS: Baicalein treatment ameliorated colitis in mice by inhibiting S1P-STAT3 signaling, suggesting that this flavonoid might be beneficial in the treatment of colitis.

15.
Bioinformatics ; 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31904845

RESUMO

MOTIVATION: Predicting the association between microRNAs (miRNAs) and diseases plays an import role in identifying human disease-related miRNAs. As identification of miRNA-disease associations via biological experiments is time-consuming and expensive, computational methods are currently used as effective complements to determine the potential associations between disease and miRNA. RESULTS: We present a novel method of neural inductive matrix completion with graph convolutional network (NIMCGCN) for predicting miRNA-disease association. NIMCGCN first uses graph convolutional networks (GCNs) to learn miRNA and disease latent feature representations from the miRNA and disease similarity networks. Then, learned features were input into a novel neural inductive matrix completion (NIMC) model to generate an association matrix completion. The parameters of NIMCGCN were learned based on the known miRNA-disease association data in a supervised end-to-end way. We compared the proposed method with other state-of-the-art methods. The AUC results showed that our method is significantly superior to existing methods. Furthermore, 50, 47, and 48 of the top 50 predicted miRNAs for three high-risk human diseases, namely, colon cancer, lymphoma, and kidney cancer, were verified using experimental literature. Finally, 100% prediction accuracy was achieved when breast cancer was used as a case study to evaluate the ability of NIMCGCN for predicting a new disease without any known related miRNAs. AVAILABILITY AND IMPLEMENTATION: https://github.com/ljatynu/NIMCGCN/. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

16.
PLoS One ; 15(1): e0227481, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31899762

RESUMO

BACKGROUND: Ischemic Stroke (IS) is a major disease which greatly threatens human health. Recent studies showed sex-specific outcomes and mechanisms of cerebral ischemic stroke. This study aimed to identify the key changes of gene expression between male and female IS in humans. METHODS: Gene expression dataset GSE22255, including peripheral blood samples, was downloaded from the Gene Expression Omnibus (GEO) dataset. Differentially Expressed Genes (DEGs) with a LogFC>1, and a P-value <0.05 were screened by BioConductor R package and grouped in female, male and overlap DEGs for further bioinformatic analysis. Gene Ontology (GO) functional annotation, Protein-Protein Interaction (PPI) network, "Molecular Complex Detection" (MCODE) modules, CytoNCA (cytoscape network centrality analysis) essential genes and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway interrelation analysis were performed. RESULTS: In a total of 54,665 genes, 185 (73 ups and 112 downs) DEGs in the female dataset, 461 DEGs (297 ups and 164 downs) in the male dataset, within which 118 DEGs overlapped (7 similar changes in female and male, 111 opposite changes in female and male) were obtained from the GSE22255 dataset. Female, male and overlapping DEGs enriched for similar cellular components and molecular function. Male DEGs enriched for divergent biological processes from female and overlapping DEGs. Sex-specific and overlapping DEGs were put into the PPI network. Overlapping genes such as IL6, presented opposite changes and were mainly involved in cytokine-cytokine receptor interactions, the TNF-signalling pathway, etc. CONCLUSION: The analysis of sex-specific DEGs from GEO human blood samples showed that not only specific but also opposite DEG alterations in the female and male stroke genome wide dataset. The results provided an overview of sex-specific mechanisms, which might provide insight into stroke and its biomarkers and lead to sex-specific prognosis and treatment strategies in future clinical practice.

17.
AAPS PharmSciTech ; 21(3): 79, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31974817

RESUMO

In order to investigate the correlation among energy input-related, drug-related, and stabilizer-related aspects for both top-down and bottom-up nanocrystal production, meloxicam nanosuspensions (NS) were produced by using three different methods (low-energy wet milling, high-pressure homogenization, and precipitation) and each method was optimized by using design of experiment (DoE). Box-Behnken design of 3 factors and 3 levels was applied for the optimization of each method. All the three models were found to be significant and the optimized process parameters were used for production of NS, respectively. Interestingly, by comparison of the top-down and bottom-up approaches, the influence of energy input (homogenization pressure or milling speed) from the instruments seemed not significant for top-down compared with bottom-up for this drug. Different mechanisms of homogenization (relatively high energy zone) and milling (relatively low energy zone) led to obtained various significant correlations for each method. Capsules containing nanocrystals were successfully produced by using a novel method applying NS (after wet bead milling and homogenization processes) as wetting agent for direct capsuling and showed superiority regarding as dissolution rate compared with the traditional two-step method (freeze-dried powder used for capsuling as the first step). Different NS preparation methodologies proved to have a direct influence on the following capsuling process and consequently, in the dissolution rate. This study also proved that residual DMSO in nanosuspension after precipitation process could affect the freeze-drying process, which might further alter the redispersion and influence the downstream processes.

18.
J Colloid Interface Sci ; 562: 363-369, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-31855799

RESUMO

Constructing high-efficiency electrocatalysts is vital towards electrocatalytic water splitting, but it remains a challenge. Although Ni-based materials have drawn extensive attention as highly active catalysts, the relatively limited electroactive sites in Ni-based catalysts still remains a great issue. In order to further boost the electrocatalytic performances, heteroatom doping and interface engineering are usually adopted for modification. Here, a new strategy is developed to construct W doped NiO/NiS2 interfaced nanosheets directly on carbon sheet, which is working as efficient and bifunctional electrocatalysts for overall water splitting. W doped NiO nanosheets are directly constructed on the carbon sheet by the hydrothermal and annealing processes. After that, W-NiO was subjected to Ar plasma assisted sulfuration treatment for forming W doped NiO/NiS2 interfaced nanosheets. Based on systematic investigations, we find that W doping can effectively induce the modified electronic structure of Ni to boost the intrinsic activities in NiO/NiS2. Further, forming NiO/NiS2 nanointerfaces can also provide rich electroactive sites and boost the charge transfer rate. Consequently, W doped NiO/NiS2 exhibits the much enhanced performances for overall water splitting. As a bifunctional electrode, W-NiO/NiS2 demonstrates a remarkable activity with a 1.614 V cell voltage at 10 mA cm-2 for overall water splitting.

19.
Bioresour Technol ; 299: 122555, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31866141

RESUMO

Increasing food waste (FW) generation has put significant pressure on the environment and has increased the global financial costs of its appropriate management. Among the traditional organic waste recycling technologies (i.e., incineration, landfilling and anaerobic digestion), composting is an economically feasible and reliable technology for FW recycling regardless of its technical flaws and social issues. The global scenario of FW generation, technical advancement in FW composting and essential nutrient recovery from organic waste with waste recycling are discussed in this article. Recent research on various strategies to improve FW composting, including co-composting, the addition of organic/inorganic additives, the mitigation of gaseous emission, and microbiological variations are comprehensively explained. Subsequently, it is shown that the performing FW composting in an existing mechanical facility can improve organic waste degradation and produce value-added mature compost to save on costs and increase the technological feasibility and viability of FW composting to some extent.

20.
J Low Genit Tract Dis ; 24(1): 21-26, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31860571

RESUMO

OBJECTIVE: The aim of the study was to compare loop electrosurgical excision procedure (LEEP) as treatment for cervical intraepithelial neoplasia (CIN) 2/3 in HIV- versus HIV+ women. MATERIALS AND METHODS: Seventy-five HIV- and 75 HIV+ women at 6 months or more after LEEP for CIN 2/3 were enrolled between September 2013 and November 2014 in this prospective cohort study at the cervical cancer screening clinic in Eldoret, Kenya. Visual inspection with acetic acid (VIA), followed by cervical cytology with conventional cytology, was performed on all women. Women with positive VIA or abnormal cervical cytology underwent colposcopy/biopsy. Lesion progression, persistence, and regression were assessed to quantify the efficacy of LEEP. RESULTS: Post-loop electrosurgical excision procedure screening test showed both a negative VIA and normal cervical cytology in 64 (85%) of HIV- and 57 (77%) HIV+ women (risk difference = 8.3%, CI = -4.2% to 21%, p = .20). Eleven (15%) HIV- and 17 (23%) HIV+ (p = .20) women had positive VIA, abnormal cervical cytology, or both and were referred for colposcopy/biopsy. Twenty-one (8 HIV-, 13 HIV+) women were biopsied. Of the 8 HIV- women, 4 (50%) had CIN lesions that regressed, 3 (38.0%) persisted, and 1 (12%) progressed to invasive cancer after LEEP. Of the 13 HIV+ women, 6 (46%) had CIN lesions that regressed, 7 (54%) had CIN lesions that persisted, and no HIV+ women had CIN lesions that progressed after LEEP. There was no difference in estimated efficacies of LEEP for HIV- and HIV+ women (92.7% versus 89.4%, risk difference = 3.3%, CI = -4.8% to 15.3%, p = .85). CONCLUSIONS: Loop electrosurgical excision procedure for CIN 2/3 is effective treatment for HIV- and HIV+ women in low-resource settings. Future efforts should improve follow-up after treatment.

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