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1.
Ultrasound Med Biol ; 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31474385

RESUMO

To investigate the feasibility of ultrasonography in detecting the oropharynx movement during deep breathing and to quantitatively analyze oropharynx airway lumen changes during deep breathing. The motions of oropharynx were monitored, and sonographic measurements of airway lumen were obtained during deep breathing in 448 healthy volunteers. Adequate visualization of oropharynx movement was obtained on all healthy volunteers. The anterior-posterior (AP) diameters and AP/transverse (T) diameter ratios were greater at the end of deep inspiration than that at the end of deep expiration for each sex (p < 0.01). The anterior-posterior dimensional changes were greater than lateral airway dimensional changes each sex (p < 0.05). Ultrasonography could provide realistic impression of the process on the oropharynx movement during deep breathing and perform the quantitative analysis of the oropharynx airway lumen changes during deep breathing. The results were encouraging and supported the utility of ultrasonography in future studies.

2.
EBioMedicine ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31474552

RESUMO

BACKGROUND: The objective of the current study was to study the molecular mechanism(s) underlying cardiac troponin I autoantibody (cTnIAAb) binding to cardiomyocyte and resultant myocardial damage/dysfunction. METHODS: cTnIAAb was purified from serum of 10 acute myocardial infarction (AMI) patients with left ventricular remodeling. Recombinant human cTnI was used to generate three mouse-derived monoclonal anti-cTnI antibodies (cTnImAb1, cTnImAb2, and cTnImAb3). The target proteins in cardiac myocyte membrane bound to cTnImAb and effect of cTnIAAb and cTnImAb on apoptosis and myocardial function were determined. FINDINGS: We found that cTnIAAb/cTnImAb1 directly bound to the cardiomyocyte membraneα-Enolase (ENO1) and triggered cell apoptosis via increased expression of ENO1 and Bax, decreased expression of Bcl2, subsequently activating Caspase8, Caspase 3, phosphatase and tensin homolog (PTEN) while inhibiting Akt activity. This cTnIAAb-ENO1-PTEN-Akt signaling axis contributed to increased myocardial apoptosis, myocardial collagen deposition, and impaired systolic dysfunction. INTERPRETATION: Results obtained in this study indicate that cTnIAAb is involved in the process of ventricular remodeling after myocardial injury. FUND: The National Natural Science Foundation of China (Grant#: 81260026).

3.
Ultrason Sonochem ; 59: 104744, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31473426

RESUMO

Ultrasonic cavitation is a physical dynamic phenomenon of bubbles inflation, compression, and collapse in liquid. A dual-frequency ultrasonic cavitation dynamics model is established in this paper to investigate dynamic evolution of bubble under single and dual frequency ultrasonic modes. The variation of bubble radius, pressure, energy, temperature, and number of water vapor molecules inside the bubble in single and dual frequency ultrasonic modes are analyzed, respectively. The results show the oscillation of cavitation bubbles is more unstable and easier to collapse in dual-frequency ultrasound field than those in single-frequency ultrasound field. With the increase of the ultrasonic frequency, cavitation effect is weakened due to the shortage of oscillation period. Under the same ultrasonic power, the maximums of bubble radius, pressure, and water vapor molecules number inside the bubble in the dual-frequency mode are larger than those in the single-frequency mode. Under the ultrasonic excited by 50 kHz + 70 kHz, the maximum bubble radius and pressure can reach 36.061 µm and 2285.9 MPa, respectively, which are much larger than 18.183 µm, 730.61 MPa at 50 kHz and 14.576 µm, 332.25 MPa at 70 kHz. The calculation results of three different frequency combinations (30 kHz + 50 kHz, 40 kHz + 60 kHz and 50 kHz + 70 kHz) indicate dual-frequency ultrasound can significantly enhance the cavitation effect.

5.
Chemosphere ; 239: 124732, 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31499304

RESUMO

A polar modified post-cross-linked poly (divinylbenzene-co-ethyleneglycol-dimethacrylate) (PCL-PDE) resin was synthesized by suspension polymerization of ethylene glycol dimethacrylate (EGDMA) and divinylbenzene (DVB), and a post-cross-linked reaction. After characterization, the adsorption behaviors of 5-hydroxymethylfurfural (5-HMF) on PCL-PDE resin were determined in comparison with the starting copolymers PDE resin. The equilibrium adsorption capacity of 5-HMF on PCL-PDE resin was much larger than PDE resin and the increase rate was greater than 52.6%. The equilibrium data of 5-HMF onto PCL-PDE resin were found to be better fitted by the Langmuir isotherm model. The kinetic data shows that the adsorption reached equilibrium in a short time (less than 20 min) can be fitted by the pore diffusion model (PDM) at various operating conditions. The effective pore diffusion coefficient was dependent upon adsorption temperature, and were 6.706 × 10-10, 8.958 × 10-10, 1.136 × 10-9 and 1.429 × 10-9 m2 s-1 at 288, 298, 308 and 318 K, respectively. Furthermore, the effects of feed flow rate (Qf = 0.6, 1.5, 3.0 and 6.0 mL min-1) and initial 5-HMF concentration (cf = 0.52, 1.02, 2.00 and 4.96 g L-1) on the adsorption were investigated systematically. Besides, a general rate model (GRM) was used to predict adsorption breakthrough curves of 5-HMF. The simulation results are highly consistent with the experimental data, indicating that the GRM can successfully simulate this process. In the desorption process, the desorption capacity reaches 99.6% of adsorbed capacity, suggesting that the PCL-PDE resin exhibited good reusability. Therefore, it could be suggested that the PCL-PDE resin has a potential application in the separation and purification of 5-HMF.

6.
New Phytol ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31514232

RESUMO

Arabidopsis CRY1 and phyB are the primary blue and red light photoreceptors mediating blue and red light inhibition of hypocotyl elongation, respectively. Auxin is a pivotal phytohormone involved in promoting hypocotyl elongation. CRY1 and phyB interact with and stabilize Aux/IAA proteins (Aux/IAAs) to inhibit auxin signaling. Here, we investigated whether photoreceptors might interact directly with Auxin Response Factors (ARFs) to regulate auxin signaling. We demonstrated by protein-protein interaction studies that CRY1 and phyB physically interact with ARF6 and ARF8 through their N-terminal domains in a blue and red light-dependent manner, respectively. Moreover, the N-terminal DNA-binding domain of ARF6 and ARF8 is involved in mediating their interactions with CRY1. Genetic studies showed that ARF6 and ARF8 act partially downstream from CRY1 and PHYB to regulate hypocotyl elongation under blue and red light, respectively. ChIP-PCR assays demonstrated that CRY1 and phyB mediate blue and red light repression of the DNA-binding activity of ARF6 and ARF6-target gene expression, respectively. Altogether, our results suggest that the direct repression of auxin-responsive gene expression mediated by the interactions of CRY1 and phyB with ARFs constitutes a new layer of the regulatory mechanisms by which light inhibits auxin-induced hypocotyl elongation.

7.
Cell Death Differ ; 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31511651

RESUMO

Centrosomal P4.1-associated protein (CPAP) is overexpressed in hepatocellular carcinoma (HCC) and positively correlated with recurrence and vascular invasion. Here, we found that CPAP plays an important role in HCC malignancies. Functional characterization indicated that CPAP overexpression increases tumor growth, angiogenesis, and metastasis ex vivo and in vivo. In addition, overexpressed CPAP contributes to sorafenib resistance. Mechanical investigation showed that the expression level of CPAP is positively correlated with activated STAT3 in HCC. CPAP acts as a transcriptional coactivator of STAT3 by directly binding with STAT3. Interrupting the interaction between CPAP and STAT3 attenuates STAT3-mediated tumor growth and angiogenesis. Overexpression of CPAP upregulates several STAT3 target genes such as IL-8 and CD44 that are involved in angiogenesis, and CPAP mRNA expression is positively correlated with the levels of both mRNAs in HCC. Knocked-down expression of CPAP impairs IL-6-mediated STAT3 activation, target gene expression, cell migration, and invasion abilities. IL-6/STAT3-mediated angiogenesis is significantly increased by CPAP overexpression and can be blocked by decreased expression of IL-8. Our findings not only shed light on the importance of CPAP in HCC malignancies, but also provide potential therapeutic strategies for inhibiting the angiogenesis pathway and treating metastatic HCC.

8.
Bioanalysis ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31512492

RESUMO

Aim: An innovative Atg4B inhibitor, S130, exhibited a negative influence on colorectal cancer cells in vitro and in vivo. To assist reliable toxicodynamic and pharmacokinetic evaluation, an LC-MS/MS assay of S130 in rat plasma must be necessary. Results: An LC-MS/MS assay for determination of S130 in rat plasma has been first developed and fully verified whose values met the admissible limits as per the US FDA guidelines. Chromatographic separation was achieved by using an isocratic elution after 3 min. MS was conducted under the ESI+ mode fitted with selected reaction monitoring. The calibration curve proved acceptable linearity over 0.50-800 ng/ml. Conclusion: The developed LC-MS/MS assay of S130 in rat plasma is easily applicable in pharmacokinetics study and the further toxicological evaluation.

9.
J Med Virol ; 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31471921

RESUMO

Gastric cancer (GC) is one of the infection-related cancers. Helicobacter pylori and Epstein-Barr virus (EBV) were established risk factors for GC. Recently, there are several reports showing the inconsistent association between hepatitis B virus (HBV) infection and the development of GC. To explore the relationship between HBV infection and the development of GC, we designed a meta-analysis of previous epidemiological studies, a hospital-based case-control study, followed by an immunohistochemistry (IHC) assay of HBV-exposed GC samples. We found that HBV infection was associated with an increased risk of GC based on the meta-analysis. No significant association between HBV infection and GC was detected according to our hospital-based case-control study. Histological examination showed that the gastric epithelium positive for HBx demonstrated a higher nuclear-cytoplasmic ratio compared to those HBx-negative cells. HBx and HBcAg were expressed more in tumors than those in normal counterparts in HBV-exposed subjects, and PD-L1 was lower in GC tissues from HBV carriers than those in HBV clearances. In conclusion, HBV infection may contribute to a higher risk for GC based on the meta-analysis and to the morphological atypia of gastric epithelium by the histological assessment, and GC patients among HBV carriers showed lower expression of PD-L1 may lose the chance for immune checkpoint blockade therapy.

10.
Appl Opt ; 58(23): 6370-6376, 2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31503784

RESUMO

In this paper, we propose a new calibration method for long-focal-length measurement using a double-grating interferometer. A numerical analysis of the relative error in measurement proves that the main factor affecting accuracy is the angle of inclination between grating grids. Hence, we present a least-squares calibration method using a series of lenses to correct this error. Experiments conducted to demonstrate high precision in wide-range focal length measurement (5-85 m) exhibited an error lower than 0.1% and a repeatability better than 0.03%.

11.
J Craniofac Surg ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31449217

RESUMO

Maxillary hypoplasia is a common developmental deformity affecting patients with cleft lip and palate. Various surgical techniques including conventional orthognathic surgery, total maxillary distraction osteogenesis, and anterior maxillary segmental distraction have been applied to address the deformity. With the evolution of 3D computed tomography imaging, the visualization of skeletal complexities in different perspectives is greatly enhanced and comprehensive surgical planning is achieved. Intraoperative efficiency is also improved with the fabrication of 3D-printed templates. The study aims to present different surgical techniques with virtual surgical planning (VSP) and 3D-printed surgical templates and the solution of representative cases. From January 2014 to January 2019, VSP was transferred to actual surgery or distraction precisely in 80 adult patients with cleft-related maxillary hypoplasia. The accuracy was analyzed and the relapse was also estimated and observed in 18 patients after 1-year follow-up. Based on our experience, VSP provides a more reliable and effective option to conventional model surgery. It facilitates the preoperative planning and accurately transfers the virtual plan to correct the cleft-related maxillary hypoplasia.

12.
Sci Rep ; 9(1): 11795, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31409884

RESUMO

Post-transplant thoracic air-leak syndrome (ALS) is rare but potentially life-threatening in patients receiving allogeneic haematopoietic stem cell transplantation (HSCT). Nevertheless, papers on thoracic ALS are limited, and this complication remains largely unknown. We reviewed 423 adult patients undergoing allogeneic HSCT from 2003 to 2014. Risk factors, clinical features and survival for thoracic ALS were collected and analysed. Thirteen out of 423 patients (3.1%) developed post-transplant thoracic ALS, including two ALS patients in the early phase. The median age at HSCT was 33 years among 13 patients with thoracic ALS. Male patients were predominant (69%). The median onset time was 253 days (range: 40-2680) after HSCT. Multivariate analysis revealed that grade III-IV acute graft-versus-host disease (GVHD) (p = 0.017), extensive chronic GVHD (cGVHD) (p = 0.019) and prior history of pulmonary invasive fungal infection (p = 0.007) were significant risk factors for thoracic ALS. In patients with cGVHD, those with thoracic ALS had a significantly worse survival than those without thoracic ALS (p = 0.04). Currently, published data analysing and exploring post-transplant thoracic ALS are limited. Our study employed a large patient cohort and determined the risk factors and clinical features for post-transplant thoracic ALS.

13.
PLoS One ; 14(8): e0221638, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31442259

RESUMO

OBJECTIVE: To explore the use of Contrast-enhanced Ultrasound (CEUS) in evaluating angiogenesis in a xenograft nasopharyngeal carcinoma (NPC) model in nude mice and the evolution of CEUS parameters according to the growth of NPC. METHODS: Nude mice were divided into three groups according to experiments conducted at various times from tumor implantation (8 mice/group; group A: 4 weeks from implantation; group B:6 weeks from implantation; group C:8 weeks from implantation). CNE-2 cells were transplanted in 24 nude mice and CEUS evaluations of the tumors were performed at 4, 6 or 8 weeks from implantation. CEUS parametric perfusion images and pathological findings were recorded. R version 3.4.4 software was used to analyze the CEUS parameters and pathological findings. RESULTS: One-way anova analysis indicated statistically significant differences among the three groups with the parameters of peak intensity (PI) (p<0.001), area wash in (AWI) (p<0.001), area wash out (AWO) (p<0.001) and tumor volumes (p<0.001).Pearson correlation coefficient analysis indicated that microvessel density (MVD) was correlated with tumor volume (r = 0.644, p = 0.001), PI (r = 0.904, p<0.0001), AWI (r = 0.547, p = 0.008) and AWO (r = 0.744, P<0.0001). Tumor volume was correlated with MVD (r = 0.644, p = 0.001), PI (r = 0.625, p = 0.002), AWI (r = 0.528, p = 0.012) and AWO (r = 0.784, p<0.001). The percentage of necrosis in histological sections was correlated with the percentage of CEUS unperfused area (r = 0.446,p = 0.038). Spearman rank correlation coefficient analysis indicated that vascular endothelial growth factor (VEGF) was correlated with PI (r = 0.462, P = 0.032). Welch t test indicated PI, AWI and AWO parameters were significantly lower than that of kidneys (p<0.001, p = 0.009, p = 0.005). CONCLUSIONS: The CEUS parameters PI, AWI and AWO indirectly reflect the MVD and the tumor volume in our model of subcutaneous transplanted NPC in nude mice, providing precious information on angiogenesis and tumor growth. VEGF may play a role in promoting angiogenesis of NPC.

14.
Biomed Chromatogr ; : e4683, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31419314

RESUMO

Dexmedetomidine has an increasingly pivotal role in sedative agents in the pediatric premedication and procedural sedation. To describe the correlation between genetic state and the concentration of dexmedetomidine, it is necessary to develop a specific, time-saved and economical method of detection in vast scale plasma samples. In the present study, an UHPLC-MS/MS method has been established and validated for detection of dexmedetomidine in pediatric population. After a simple liquid-liquid extraction with organic solution, the analytes were separated on Acquity BEH C18 column (2.1 mm × 50 mm,1.7µm particle size, Waters, USA) by gradient elution with the mobile phase of acetonitrile, 1‰ aqueous formic acid with a flow rate of 0.3 mL·min-1 . The mass spectrometry was under the positive selective reactions monitoring mode and the mass transitions monitored were m/z 201.3→95.1, 204.2→98.0 for dexmedetomidine and deuterated medetomidine (IS), respectively. The full validated method was satisfied with acceptable limits. This method using a stable isotope-labeled internal standard was specific and has been successfully used to detect the dexmedetomidine in plasma samples from 260 pediatric patients. The subsequent application to pharmacogenetic study was described. Specifically, it is the first found that CYP2A6 rs835309 was closely associated with the concentration of dexmedetomidine.

15.
Science ; 365(6453): 578-582, 2019 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-31395780

RESUMO

Dielectric capacitors with ultrahigh power densities are fundamental energy storage components in electrical and electronic systems. However, a long-standing challenge is improving their energy densities. We report dielectrics with ultrahigh energy densities designed with polymorphic nanodomains. Guided by phase-field simulations, we conceived and synthesized lead-free BiFeO3-BaTiO3-SrTiO3 solid-solution films to realize the coexistence of rhombohedral and tetragonal nanodomains embedded in a cubic matrix. We obtained minimized hysteresis while maintaining high polarization and achieved a high energy density of 112 joules per cubic centimeter with a high energy efficiency of ~80%. This approach should be generalizable for designing high-performance dielectrics and other functional materials that benefit from nanoscale domain structure manipulation.

16.
Phytomedicine ; 64: 153060, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31401495

RESUMO

BACKGROUD: Diabetic nephropathy is the most serious complication of diabetes. Cyclocarya paliurus (CP), an herbal plant in China, has been reported the biological activity of anti-hyperglycemia. However, its effects on the diabetic nephropathy (DN) remain unclear. PURPOSE: We aimed to investigate the potential role of CP and its underlying mechanisms on DN. STUDY DESIGN: In this study, the effects of triterpenic acids-enriched fraction from CP (CPT) on DN was evaluated in streptozotocin (STZ)-induced rats and high glucose (HG)-induced HK-2 cells models. METHODS: After oral administration with or without CPT for 10 weeks, body weight, glucose, microalbumin, serum creatinine and blood urea in STZ-induced rats were detected. Histological analysis was performed to evaluate renal function of mice. Moreover, the level of autophagy was detected by western blot or immunostaining. In vitro, HG-induced HK-2 cell was conducted to evaluate the renal protection and mechanism of CPT. RESULTS: CPT dramatically decreased the levels of microalbumin, serum creatinine and blood urea nitrogen and ameliorated increased mesangial matrix and glomerular fibrosis. In addition, we found the CPT prevented renal damage and cell apoptosis through the autophagy. Furthermore, CPT could increase the phosphorylation of AMPK and decrease its downstream effector phosphorylation of mTOR. Besides, the expression of LC3-II were locked by AMPK inhibitor dorsomorphin dihydrochloride (compound C), implying that the autophagy may be regulated with AMPK activation. CONCLUSION: These findings suggested that CPT might be a desired candidate against diabetes, potentially through AMPK-mTOR-regulated autophagy pathway.

17.
Yi Chuan ; 41(8): 703-715, 2019 Aug 20.
Artigo em Chinês | MEDLINE | ID: mdl-31447421

RESUMO

Hybrid rice has contributed greatly to global food security. Cytoplasmic male sterility (CMS) and photo/ thermo sensitive genic male sterility (P/TGMS) are genetic bases for three-line and two-line hybrid rice production, respectively. In contrast, (sub-) specific hybrid sterility (HS) is a major barrier for utilization of hybrid vigor of distant hybrid rice. Therefore, understanding the molecular regulatory mechanism of rice fertility is a key technical issue for hybrid rice industry, and a long-standing basic scientific issue for nuclear-cytoplasmic interaction and reproductive isolation. Chinese geneticists of plant sciences have made tremendous contributions on the molecular genetic basis of rice fertility related to hybrid rice production. Here, we review the development of hybrid rice production systems in China and summarize current advance on genetic basis and molecular mechanism of CMS, P/TGMS, and HS involved in hybrid rice. We also discuss problems of hybrid rice production in China and point out new direction for future utilization of heterosis in rice.


Assuntos
Hibridização Genética , Oryza/genética , Oryza/fisiologia , Infertilidade das Plantas/genética , China , Fertilidade/genética , Vigor Híbrido
18.
Biosci Rep ; 39(9)2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31416885

RESUMO

Preeclampsia (PE) is a disorder of pregnancy that is characterised by hypertension and a significant amount of proteinuria beginning after 20 weeks of pregnancy. It is closely associated with high maternal morbidity, mortality, maternal organ dysfunction or foetal growth restriction. Therefore, it is necessary to identify early and novel diagnostic biomarkers of PE. In the present study, we performed a multi-step integrative bioinformatics analysis of microarray data for identifying hub genes as diagnostic biomarkers of PE. With the help of gene expression profiles of the Gene Expression Omnibus (GEO) dataset GSE60438, a total of 268 dysregulated genes were identified including 131 up- and 137 down-regulated differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEGs suggested that DEGs were significantly enriched in disease-related biological processes (BPs) such as hormone activity, immune response, steroid hormone biosynthesis, metabolic pathways, and other signalling pathways. Using the STRING database, we established a protein-protein interaction (PPI) network based on the above DEGs. Module analysis and identification of hub genes were performed to screen a total of 17 significant hub genes. The support vector machines (SVMs) model was used to predict the potential application of biomarkers in PE diagnosis with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.958 in the training set and 0.834 in the test set, suggesting that this risk classifier has good discrimination between PE patients and control samples. Our results demonstrated that these 17 differentially expressed hub genes can be used as potential biomarkers for diagnosis of PE.

19.
Curr Diab Rep ; 19(9): 74, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31375932

RESUMO

PURPOSE OF REVIEW: We discuss opportunities to address key barriers to widespread implementation of teleophthalmology programs for diabetic eye screening in the United States (U.S.). RECENT FINDINGS: Teleophthalmology is an evidence-based form of diabetic eye screening. This technology has been proven to substantially increase diabetic eye screening rates and decrease blindness. However, teleophthalmology implementation remains limited among U.S. health systems. Major barriers include financial concerns as well as limited utilization by providers, clinical staff, and patients. Possible interventions include increasingly affordable camera technology, demonstration of financially sustainable billing models, and engaging key stakeholders. Significant opportunities exist to overcome barriers to scale up and promote widespread implementation of teleophthalmology in the USA. Further development of methods to sustain effective increases in diabetic eye screening rates using this technology is needed. In addition, the demonstration of cost-effectiveness in a variety of billing models should be investigated to facilitate widespread implementation of teleophthalmology in U.S. health systems.

20.
Sheng Wu Gong Cheng Xue Bao ; 35(8): 1401-1410, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31441611

RESUMO

Gene editing is a technique for modifying gene fragments. The novel gene editing technology focuses on the field of artificial nuclease cleavage technology, mainly ZFN technology, TALEN technology, CRISPR technology and base editing technology. The continuous improvement of gene editing technology has promoted the rapid development of agriculture, animal husbandry and biomedicine, but at the same time, technical defects and ethical controversy have brought enormous challenges to its own development. This article will briefly discuss the development and challenges of gene editing technology, as well as the views at home and abroad, and hope to inspire readers to recognize gene editing technology.


Assuntos
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas , Edição de Genes , Agricultura , Animais , Endonucleases
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