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1.
Neural Regen Res ; 17(1): 130-136, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34100448

RESUMO

Our previous study showed that cell cycle exit and neuronal differentiation 1 (CEND1) may participate in neural stem cell cycle exit and oriented differentiation. However, whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear. In this study, we performed quantitative proteomic analysis and found that after traumatic brain injury, CEND1 expression was downregulated in mouse brain tissue. Three days after traumatic brain injury, we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site. We found that at 5 weeks after traumatic brain injury, transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function. In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells, but significantly promoted their neuronal differentiation. Additionally, CEND1 overexpression reduced protein levels of Notch1 and cyclin D1, but increased levels of p21 in CEND1-transfected neural stem cells. Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection. These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury. This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University, China (approval No. 2016034) on November 25, 2016.

2.
Sci Total Environ ; 803: 150008, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34482130

RESUMO

The intensifying globalization contributes to the anthropogenic methane (CH4) emissions outsourcing, a strong greenhouse gas and harmful air pollutant, through the increasingly complex global trade network. However, the CH4 flow patterns embodied in global traded goods and services have not been interpreted from the perspective of a complex network. In this paper, we integrate global CH4 emission inventory from the EDGAR (the Emission Database for Global Atmospheric Research) databases, global multi-regional input-output model from the GTAP database, and complex network analysis to reveal the structural characteristics of the global CH4 flow network (GCFN). In the GCFN, more than one quarter of the global anthropogenic CH4 emissions in 2014 are associated with international trade. The top 20 economies contribute to about 70% of the total embodied CH4 emission flows. The GCFNs mainly consist of tripartite patterns centered on China, the USA and Russia. Some emerging countries, such as Thailand and Brazil, also exhibit dominated positions in different kinds of GCFNs. Moreover, the core-periphery structure of the GCFN confirms the existence of a few hub economies associated with a large amount of CH4 emissions. The results emphasize the multinational cooperation on global CH4 emission mitigation, and well-focused mitigation policies should be implemented on some key economies.


Assuntos
Gases de Efeito Estufa , Serviços Terceirizados , Comércio , Internacionalidade , Metano/análise
3.
Food Chem ; 371: 131388, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34808779

RESUMO

In recent years, the safety of copper in drinking water has increasingly been questioned. Copper speciation is an important factor that affects its bioavailability and toxicity; thus, it is critical to investigate the speciation of copper that is ingested from food and drinking water during in vitro digestion. After digestion, water- and food-derived copper formed 60 ± 4% 0.1-1 kDa and 49 ± 6% 10-1,000 kDa copper complexes, respectively. Under simulated fasting drinking water conditions, up to 90 ± 2% 0.1-1 kDa copper complexes formed. In addition, using ion selective electrode analysis, water-derived copper was detected that contained higher Cu2+ concentrations after digestion than those of food-derived copper. These results indicate that water-derived copper forms smaller-sized species and exhibits higher Cu2+ concentrations during digestion than those of food-derived copper, thereby highlighting the importance of reassessing the safety limit for copper in drinking water.

4.
Bioresour Technol ; 343: 126132, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34655787

RESUMO

A novel double-anammox process for advanced mainstream nitrogen removal was established using step-feed sequencing batch reactor (SBR) system with integration of suspend sludge and biofilms. Following optimization of influent distribution ratio, the effluent total inorganic nitrogen (TIN) was < 10.2 mg N/L, with influent TIN of 43.4 mg N/L, and anammox contributed 71.4% to TIN removal. Biological processes and batch tests revealed that gradient C/N reduction promoted denitratation/anammox in anoxic stage, and simultaneous nitritation and anammox were achieved in oxic stage. Specially, anammox maintained on biofilms with abundance over 109 copies/ (g dry sludge). High-throughput sequencing revealed that Thauera and Nitrosomonas were enriched in flocs. Furthermore, metagenomic sequencing confirmed that Thauera owns narG and napA (NO3-→NO2-) and Nitrosomonas owns amoA (NH4+→NO2-), support stable NO2- supply for double-anammox. This mainstream anammox-dominant process could potentially be used for stable nitrogen removal in municipal wastewater treatment plants.

5.
J Hazard Mater ; 423(Pt A): 126866, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34482079

RESUMO

Antibiotic resistant bacteria (ARB) and the antibiotic resistance genes (ARGs) dissemination via plasmid-mediated conjugation have attracted considerable attentions. In this research, sulfidated nanoscale zerovalent iron (S-nZVI)/peroxymonosulfate (PMS) and S-nZVI/peroxydisulfate (PDS) process were investigated to inactivate ARB (Escherichia coli DH5α with RP4 plasmid, Pseudomonas. HLS-6 contains sul1 and intI1 on genome DNA sequence). S-nZVI/PMS system showed higher efficiency than S-nZVI/PDS on ARB inactivation. Thus, the optimal condition 28 mg/L S-nZVI coupled with 153.7 mg/L (0.5 mM) PMS was applied to remove both intracellular ARGs (iARGs) and ARB. The oxidative damage of ARB cell was systemically studied by cell viability, intracellular Mg2+ levels, the changes of extracellular and internal structure, integrity of cell walls and membranes and enzymatic activities. S-nZVI/PMS effectively inactivated ARB (~7.32 log) within 15 min. These effects were greatly higher than those achieved individually. Moreover, removal efficiencies of iARGs sul1, intI1 and tetA were 1.52, 1.79 and 1.56 log, respectively. These results revealed that S-nZVI and PMS have a synergistic effect against ARB and iARGs. The regrowth assays illustrated that the ARB were effectively inactivated. By verifying the inhibitory impacts of S-nZVI/PMS treatment on conjugation transfer, this work highlights a promising alternative technique for inhibiting the horizontal gene transfer.

6.
Sci Total Environ ; 804: 150199, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34520918

RESUMO

Flooded condition enhances arsenic (As) mobility in paddy soils, posing an imminent threat to food safety and human health. Hence, iron oxyhydroxide and iron oxyhydroxide sulfate-modified coal gangue (CG-FeOH and CG-FeOS) were synthesized for remediation of As-contaminated paddy soils under a flooded condition. Compared to the control, CG-FeOH and CG-FeOS application decreased the soil pH by 0.10-0.80 and 0.13-1.63 units, respectively. CG-FeOH and CG-FeOS application significantly (P < 0.05) decreased available As concentration by 13.46-43.44% and 21.31-54.37%, respectively. CG-FeOH and CG-FeOS significantly (P < 0.05) reduced the non-specifically adsorbed and specifically adsorbed As fractions and increased As(V) proportion by 22.61-26.53% and 29.10-36.51%, respectively. Our results showed that CG-FeOH and CG-FeOS could change As geochemical fraction and valence state, consequently reducing available As concentration in paddy soils. Moreover, the sulfate could enhance the oxidation and co-precipitation of As with CG-FeOH. Compared to CG-FeOH, CG-FeOS was more effective in decreasing available As concentration and oxidizing As(III) to As(V). This study revealed that CG-FeOS is a potential amendment for As immobilization in paddy soils.


Assuntos
Arsênio , Oryza , Poluentes do Solo , Arsênio/análise , Carvão Mineral , Compostos Férricos , Humanos , Ferro/análise , Solo , Poluentes do Solo/análise , Sulfatos
7.
Neural Regen Res ; 17(5): 1115-1124, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34558540

RESUMO

Multiple sclerosis is associated with structural and functional brain alterations leading to cognitive impairments across multiple domains including attention, memory, and the speed of information processing. The hippocampus, which is a brain important structure involved in memory, undergoes microstructural changes in the early stage of multiple sclerosis. In this study, we analyzed hippocampal function and structure in patients with relapsing-remitting multiple sclerosis and explored correlations between the functional connectivity of the hippocampus to the whole brain, changes in local brain function and microstructure, and cognitive function at rest. We retrospectively analyzed data from 20 relapsing-remitting multiple sclerosis patients admitted to the Department of Neurology at the China-Japan Union Hospital of Jilin University, China, from April 2015 to November 2019. Sixteen healthy volunteers were recruited as the healthy control group. All participants were evaluated using a scale of extended disability status and the Montreal cognitive assessment within 1 week before and after head diffusion tensor imaging and functional magnetic resonance imaging. Compared with the healthy control group, the patients with relapsing-remitting multiple sclerosis had lower Montreal cognitive assessment scores and regions of simultaneously enhanced and attenuated whole-brain functional connectivity and local functional connectivity in the bilateral hippocampus. Hippocampal diffusion tensor imaging data showed that, compared with the healthy control group, patients with relapsing-remitting multiple sclerosis had lower hippocampal fractional anisotropy values and higher mean diffusivity values, suggesting abnormal hippocampal structure. The left hippocampus whole-brain functional connectivity was negatively correlated with the Montreal cognitive assessment score (r = -0.698, P = 0.025), and whole-brain functional connectivity of the right hippocampus was negatively correlated with extended disability status scale score (r = -0.649, P = 0.042). The mean diffusivity value of the left hippocampus was negatively correlated with the Montreal cognitive assessment score (r = -0.729, P = 0.017) and positively correlated with the extended disability status scale score (r = 0.653, P = 0.041). The right hippocampal mean diffusivity value was positively correlated with the extended disability status scale score (r = 0.684, P = 0.029). These data suggest that the functional connectivity and presence of structural abnormalities in the hippocampus in patients with relapse-remission multiple sclerosis are correlated with the degree of cognitive function and extent of disability. This study was approved by the Ethics Committee of China-Japan Union Hospital of Jilin University, China (approval No. 201702202) on February 22, 2017.

8.
Front Genet ; 12: 753953, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721541

RESUMO

Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop that has strong resistance to abiotic stress. While heat shock protein 20 (HSP20) has been implicated in vegetable response to heat stress, little is known regarding activity of HSP20 family proteins in C. moschata. Here, we performed a comprehensive genome-wide analysis to identify and characterize the functional dynamics of the Cucurbita moschata HSP20 (CmoHSP20) gene family. A total of 33 HSP20 genes distributed across 13 chromosomes were identified from the pumpkin genome. Our phylogenetic analysis determined that the CmoHSP20 proteins fell into nine distinct subfamilies, a division supported by the conserved motif composition and gene structure analyses. Segmental duplication events were shown to play a key role in expansion of the CmoHSP20 gene family. Synteny analysis revealed that 19 and 18 CmoHSP20 genes were collinear with those in the cucumber and melon genomes, respectively. Furthermore, the expression levels of pumpkin HSP20 genes were differentially induced by heat stress. The transcript level of CmoHSP20-16, 24 and 25 were down-regulated by heat stress, while CmoHSP20-7, 13, 18, 22, 26 and 32 were up-regulated by heat stress, which could be used as heat tolerance candidate genes. Overall, these findings contribute to our understanding of vegetable HSP20 family genes and provide valuable information that can be used to breed heat stress resistance in cucurbit vegetable crops.

9.
Front Psychol ; 12: 747039, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777138

RESUMO

Previous studies have suggested that reading stories of role models can improve the growth mindsets of students. The current study aimed to investigate the types of stories that can increase the growth mindsets of high school students, undergraduates, and graduates and how many specific stories undergraduates with low and high perseverance need to read to improve their growth mindsets. In study 1, high school students, undergraduates, and graduates were assigned to read either five struggle stories or five achievement stories of role models. Their mindsets were measured before and after reading the stories. The results showed that reading struggle stories rather than achievement stories of role models increased the growth mindsets of undergraduates and graduates. In study 2, undergraduates with high and low perseverance were assigned to read five struggle stories or five achievement stories of role models. Their mindsets were measured before reading stories and after reading each story. The results showed that the growth mindsets of undergraduates with low perseverance increased after reading two struggle stories of role models, and increased further after reading five struggle stories of role models. More importantly, the level of growth mindsets of undergraduates with low perseverance was equal to that of undergraduates with high perseverance after reading five struggle stories of role models. These findings reveal that reading struggle stories of role models can improve the growth mindsets of undergraduates and graduates. The personality of students affects the effectiveness of story-based mindset intervention.

10.
Front Oncol ; 11: 604428, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34778020

RESUMO

Objectives: This study was conducted in order to explore the value of histogram analysis of the intravoxel incoherent motion-kurtosis (IVIM-kurtosis) model in the diagnosis and grading of prostate cancer (PCa), compared with monoexponential model (MEM). Materials and Methods: Thirty patients were included in this study. Single-shot echo-planar imaging (SS-EPI) diffusion-weighted images (b-values of 0, 20, 50, 100, 200, 500, 1,000, 1,500, 2,000 s/mm2) were acquired. The pathologies were confirmed by in-bore MR-guided biopsy. The postprocessing and measurements were processed using the software tool Matlab R2015b for the IVIM-kurtosis model and MEM. Regions of interest (ROIs) were drawn manually. Mean values of D, D*, f, K, ADC, and their histogram parameters were acquired. The values of these parameters in PCa and benign prostatic hyperplasia (BPH)/prostatitis were compared. Receiver operating characteristic (ROC) curves were used to investigate the diagnostic efficiency. The Spearman test was used to evaluate the correlation of these parameters and Gleason scores (GS) of PCa. Results: For the IVIM-kurtosis model, D (mean, 10th, 25th, 50th, 75th, 90th), D* (90th), and f (10th) were significantly lower in PCa than in BPH/prostatitis, while D (skewness), D* (kurtosis), and K (mean, 75th, 90th) were significantly higher in PCa than in BPH/prostatitis. For MEM, ADC (mean, 10th, 25th, 50th, 75th, 90th) was significantly lower in PCa than in BPH/prostatitis. The area under the ROC curve (AUC) of the IVIM-kurtosis model was higher than MEM, without significant differences (z = 1.761, P = 0.0783). D (mean, 50th, 75th, 90th), D* (mean, 10th, 25th, 50th, 75th), and f (skewness, kurtosis) correlated negatively with GS, while D (kurtosis), D* (skewness, kurtosis), f (mean, 75th, 90th), and K (mean, 75th, 90th) correlated positively with GS. The histogram parameters of ADC did not show correlations with GS. Conclusion: The IVIM-kurtosis model has potential value in the differential diagnosis of PCa and BPH/prostatitis. IVIM-kurtosis histogram analysis may provide more information in the grading of PCa than MEM.

11.
Front Cardiovasc Med ; 8: 656536, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34778384

RESUMO

Aims: HFA-PEFF score has been proposed for diagnosing heart failure with preserved ejection fraction (HFpEF). Currently, there are only a limited number of tools for predicting the prognosis. In this study, we evaluated whether the HFA-PEFF score can predict mortality in patients with HFpEF. Methods: This single-center, retrospective observational study enrolled patients diagnosed with HFpEF at the First Affiliated Hospital of Dalian Medical University between January 1, 2015, and April 30, 2018. The subjects were divided according to their HFA-PEFF score into low (0-2 points), intermediate (3-4 points), and high (5-6 points) score groups. The primary outcome was all-cause mortality. Results: A total of 358 patients (mean age: 70.21 ± 8.64 years, 58.1% female) were included. Of these, 63 (17.6%), 156 (43.6%), and 139 (38.8%) were classified into the low, intermediate, and high score groups, respectively. Over a mean follow-up of 26.9 months, 46 patients (12.8%) died. The percentage of patients who died in the low, intermediate, and high score groups were 1 (1.6%), 18 (11.5%), and 27 (19.4%), respectively. A multivariate Cox regression identified HFA-PEFF score as an independent predictor of all-cause mortality [hazard ratio (HR):1.314, 95% CI: 1.013-1.705, P = 0.039]. A Cox analysis demonstrated a significantly higher rate of mortality in the intermediate (HR: 4.912, 95% CI 1.154-20.907, P = 0.031) and high score groups (HR: 5.291, 95% CI: 1.239-22.593, P = 0.024) than the low score group. A receiver operating characteristic (ROC) analysis indicated that the HFA-PEFF score can effectively predict all-cause mortality after adjusting for age and New York Heart Association (NYHA) class [area under the curve (AUC) 0.726, 95% CI 0.651-0.800, P = 0.000]. With an HFA-PEFF score cut-off value of 3.5, the sensitivity and specificity were 78.3 and 54.8%, respectively. The AUC on ROC analysis for the biomarker component of the score was similar to that of the total score. Conclusions: The HFA-PEFF score can be used both to diagnose HFpEF and predict the prognosis. The higher scores are associated with higher all-cause mortality.

12.
J Sep Sci ; 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34784448

RESUMO

Rhubarb is a widely used HM. To achieve different effects, rhubarb is usually steamed with rice wine (steamed rhubarb). This steaming treatment increases the blood-activating and stasis-removing effects of rhubarb. A specific and accurate ultra-HPLC-MS/MS method was established for simultaneous determination of anthraquinone glycosides, aglycones and glucuronic acid metabolites in plasma and tissues after administration of raw rhubarb and steamed rhubarb in blood stasis rats. Chromatographic separation was performed on ACQUITY ultra-HPLC BEH Shield RP 18 column using the mobile phase consisting of water and acetonitrile both containing 0.1% formic acid. Satisfactory linearity, precision, accuracy, extraction recovery and matrix effect have been achieved. From pharmacokinetic study, it showed that glucuronic acid metabolites were found abundantly in plasma as bioactive components. The lower area under concentration-time curve, maximum concentration and higher apparent volume of distribution (P<0.01), body clearance (P<0.01) values in steamed rhubarb showed that most components of steamed rhubarb have lower bioavailability in plasma compared with raw rhubarb. But it found these components were mainly distributed in spleen and liver with large blood flow and perfusion rates. The pharmacokinetics and tissue distribution studies of anthraquinone components will provide helpful information for clinical application of steamed rhubarb and raw rhubarb. This article is protected by copyright. All rights reserved.

14.
World J Pediatr ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34787828

RESUMO

BACKGROUND: The clinical use of gentamicin always lies in its antimicrobial activity in the past as an aminoglycoside antibiotic. However, in the past decade, there were considerable interests in therapeutic approaches in treating hereditary diseases. Some of the genodermatosis is caused by nonsense mutations that create premature termination codons and lead to the production of truncated or non-functional proteins. Gentamicin could induce readthrough of nonsense mutations and enable the synthesis of full-length proteins. We focus on previous publications on topical application of gentamicin and review its utility in genetic skin diseases. DATA SOURCES: We search the MEDLINE through PubMed, EMBASE databases, and the Clinical Trials Registry Platform from January 1960 to July 2020 using the key search terms "gentamicin, topical gentamicin, genodermatosis, genetic skin diseases". RESULTS: The application of gentamicin in genodermatosis yielded promising results, both in vivo and in vitro, including Nagashima-type palmoplantar keratosis, epidermolysis bullosa, Hailey-Hailey disease, hereditary hypotrichosis simplex of the scalp, etc. CONCLUSIONS: Topical gentamicin is a potential treatment option for genodermatosis caused by nonsense mutation.

15.
Waste Manag ; 137: 39-49, 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34731679

RESUMO

Anisotropy of hydraulic conductivity is an important parameter controlling fluid movement in municipal solid waste (MSW) landfills, while measurements of anisotropy are rare. In this study, a laboratory-scale enhanced reactor was built to create MSW samples with different degrees of degradation. Vertical and horizontal hydraulic conductivities of these samples were measured in a self-designed permeameter to study the effects of compression and degradation on anisotropy of MSW. CT scanning was performed to observe the internal pore-structure of MSW under compression. A prediction model of anisotropy under compression was established. It was found that as degradation time increased from 0 month to 18 months, the dry mass percent of 0D particles increased from 12.3% to 38.8%, while 2D particles content decreased from 78.7% to 47.2%. As vertical stress increased from 50 kPa to 400 kPa, dry unit weight (γd) increased from 3.26 kN/m3 to 5.51 kN/m3, anisotropy (A) increased from 1.26 to 5.17. It was because that the size and continuity of pores decreased and the angle of pore arrangement tended to be horizontal as the vertical stress increased. The relation between anisotropy and vertical stress could be well fitted with the prediction model. When degradation time increased from 0 month to 18 months, A decreased linearly from 5.02 to 2.75 due to the decreasing content of 2D particles. Anisotropy also decreased with the decreasing C/L. Compression has much greater influence on waste anisotropy than that of degradation. Anisotropy of MSW at different depths of landfills could be determined based on the trend lines in this study.

16.
J Hazard Mater ; 424(Pt C): 127611, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34740163

RESUMO

Vegetations play a vital role in the ecological function of constructed wetlands (CW), but the systemic phytoremediation mechanism of CW is still unclear. An integrated vertical-flow constructed wetland (IVCW) was established to elucidate the phytoremediation mechanisms and plants eco-physiological response to an emerging contaminant, sulfamethoxazole (SMX). Attenuation of SMX in IVCW with and without vegetation (Acorus calamus) are comparatively analyzed. The results showed significant enhancement of removal efficiencies of total nitrogen (via intensified denitrification) and SMX by up to 10% respectively with vegetation. A unique micro-rhizo environment was created by stimulating the denitrifiers, Clostridium_sensu_stricto, Ignavibacterium, Rhodanobacter, and Geobacter. Free-living plant growth-promoting bacteria, unclassified_Burkholderiales and unclassified_Betaproteobacteria, proliferated in the rhizosphere, protecting the growth mechanism of A. calamus and, consequently, promoting performance of the IVCW. Overall, A. calamus exhibited tolerance to SMX, maintaining its photosynthesis rate and stabilizing the plant cell structure by an effective antioxidant system. The growth and defense mechanisms of A. calamus appeared to positively correlate with the IVCW performance, whereby the photosynthetic rate and antioxidant enzymes activities peaked together with the maximum removal efficiency of TN (77.81%) and SMX (99.88%). The contribution of vegetation to ecotoxicity reduction in CW might be underrated as absorbed SMX could be phytodegraded into less toxic metabolites via specific enzymes.

17.
J Colloid Interface Sci ; 608(Pt 2): 2088-2099, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34763289

RESUMO

Nitrophenols (NPs) are widely used in industries and highly toxic to ecological environment and human health. Because aminophenols (APs) are important chemicals, catalytic reduction of NPs via efficient and environment-friendly strategies is of great importance. Herein, we developed a green photocatalysis route to efficiently convert NPs to APs using a Fe (II) modified graphitic carbon nitride (g-C3N4) photocatalyst, where phenanthroline units were employed to bridge Fe (II) and carbon nitride framework. The optimized sample P-CN-8-Fe presented significantly improved absorption of visible light, separation of photogenerated charges and carrier transportation in comparison with the pristine g-C3N4 and the modified samples of CN-Fe and P-CN-8. Accordingly, the P-CN-8-Fe showed a high conversion (97%) of p-nitrophenol (p-NP) to p-aminophenol (p-AP) under 2 h visible light irradiation, and meanwhile possessed high photocatalytic durability. Its high activity was also demonstrated through photocatalytic reduction of other NPs and nitrobenzene compounds. Finally, a possible mechanism was proposed for the photocatalytic reduction of p-NP by P-CN-8-Fe. This work provides an effective approach to prepare Fe (II) modified g-C3N4 through the bridging effect of phenanthroline group, which is a potential visible light driven photocatalyst for reduction of nitrobenzene derivatives.

18.
Ecotoxicol Environ Saf ; 228: 112987, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34781129

RESUMO

Increasing evidence shows that human exposure to bisphenols can increase the risk of allergic disease, such as child asthma. However, the mechanism by which exposure to bisphenols causes allergic disease is unclear. In addition, the effects of exposure to bisphenols during pregnancy on infantile eczema have been poorly studied. The aim of our study was to investigate the effect of bisphenols (BPA, BPF and BPS) exposure during pregnancy on immune cells in cord blood, and on the occurrence of infantile eczema. 111 mother-child pairs with urine samples from pregnant women and cord blood were recruited from a birth cohort established in February 2019 in Shenyang, China. The levels of urinary bisphenols and Th1-, Th2-, Treg- and Th17-related genes, and cytokines in cord blood, as well as the incidence of infantile eczema at 6 and 12 months follow up were determined. Our results show that BPA, BPF and BPS were detected in 100%, 63.1% and 46.8% of the urine samples, respectively. The median concentration of urine specific gravity adjusted BPA (SG-BPA) was 7.46 ng/mL. High SG-BPA levels during pregnancy was independently associated with increased risk of infantile eczema (adjusted OR = 2.731, 95%CI: 1.064-7.012, P = 0.037). Higher levels of FOXP3 gene in cord blood had a significantly lower risk of developing eczema in infants (adjusted OR=0.430, 95%CI: 0.190-0.972, P = 0.042). However, BPS and BPF levels were not associated with infantile eczema. FOXP3 gene levels in cord blood mediated the relationship between SG-BPA levels during pregnancy and infantile eczema (indirect effect: ß = 0.350 [CI:0.011,1.077]). Our findings indicate that high levels of BPA exposure during pregnancy increase the risk of infantile eczema, which may be associated with down-regulation of FOXP3 gene expression in cord blood.

19.
Int J Biol Sci ; 17(15): 4192-4206, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34803492

RESUMO

Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271+ BM-derived mononuclear cells (BM-MNCs) from two human subjects. We successfully identified LEPRhiCD45low BM-MSCs within the CD271+ BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. Biological functional annotations of the transcriptomes suggest that osteoblast precursors induce angiogenesis coupled with osteogenesis, and chondrocyte precursors have the potential to differentiate into myocytes. We also discovered transcripts for several clusters of differentiation (CD) markers that were either highly expressed (e.g., CD167b, CD91, CD130 and CD118) or absent (e.g., CD74, CD217, CD148 and CD68) in BM-MSCs, representing potential novel markers for human BM-MSC purification. This study is the first systematic in vivo dissection of human BM-MSCs cell subtypes at the single-cell resolution, revealing an insight into the extent of their cellular heterogeneity and roles in maintaining bone homeostasis.

20.
Food Chem X ; 12: 100155, 2021 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-34816121

RESUMO

In the production of canned citrus, large amounts of processing water were discharged during the segment membrane removal process, causing severe pollution. In order to reduce pollution and recover the bioactive compounds in the processing water, the production of canned satsuma mandarin, sweet orange and grapefruit were studied, and improved acid (0.1% HCl, 0.4% citric acid) and alkali (0.1% KOH, 0.2% NaOH) were used to conduct the new chemical hydrolysis process to remove the segment membrane. The obtained acid and alkali processing water were firstly explored the potential to make novel beverages, which contain electrolytes (Na: 472-945 ppm; K: 208-279 ppm; Cl: 364-411 ppm; citrate: 1105-1653 ppm) and potential prebiotics such as pectin and flavonoids. The improved segment membrane removal process realized the conversion of wastewater into drinkable beverages at low costs. The bioactive compounds were fully recovered without wastewater discharging, which produced great environmental, economic and health value.

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