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1.
Chemosphere ; 286(Pt 2): 131826, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34426141

RESUMO

Pesticides play critical roles in agricultural fields; however, pesticide residues can cause serious damage to human health and the ecological environment; therefore, developing a rapid and sensitive method for pesticide detection is urgently needed. Nanostructure-assisted matrix laser desorption/ionization (MALDI) mass spectrometry (MS) has great potential for the detection of low-mass pesticides. In this study, a novel Ti3C2 MXene nanowire (TMN) was prepared by a facile sol-gel method and served as a matrix to enhance MALDI MS performance in the analysis of pesticides in positive ion mode. The TMN showed superior performance in the high-throughput detection of six kinds of pesticides (organophosphorus, organochlorine, carbamate, neonicotinoids, triazole, and oxadiazines), with ultrahigh sensitivity (detection limits at sub-ppt levels), remarkable repeatability, excellent salt tolerance, and extremely low background compared to traditional organic matrices due to the specific polyaromatic structure and the doping of nitrogen. Furthermore, this matrix was successfully employed for the analysis of residual pesticides in traditional Chinese herbs, and the level of diniconazole was quantified with a linear range of 0-50 ng/mL (R2 > 0.99). More importantly, the spatial distribution of various endogenous compounds (e.g., amino acids and saccharides, fatty acids, alkaloids, and plant hormones) and xenobiotic pesticides from the intact root of the medicinal plant P. quinquefolium was clearly visualized using the TMN self-assembly film as a matrix for MALDI imaging mass spectrometry (IMS). With superior advantages such as sensitivity, simplicity, rapidness, and minimal sample requirement, TMN as a matrix-assisted MALDI MS shows great promise for various applications.


Assuntos
Nanofios , Praguicidas , Ensaios de Triagem em Larga Escala , Humanos , Lasers , Praguicidas/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Titânio
2.
Sex Health ; 18(5): 405-412, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34782058

RESUMO

Background The 'Down to Test (DTT)' campaign is a sexually transmissible infection (STI) social marketing intervention delivered through outdoor music festival activations and supported by digital media communications in New South Wales, Australia. This paper investigates whether and how the tailored messages reached the intended audience. Methods Data was collected through three annual rounds of online surveys post campaign exposure, targeting young people (aged 15-29years) attending 14 music festivals in NSW from October 2017 to March 2020. Descriptive statistics, principal component analysis and multivariable logic regression were applied to identify the key client segment and factors associated with a strong intention for future STI screening. Results Of the 10044 participants with a valid urine specimen submitted, 261 (2.8%) tested positive for chlamydia. Altogether, 1776 participants (median age=22) self-completed the evaluation surveys online with more being female (73.4%) than male (26.2%). Participants were mostly Australian-born (89.5%), heterosexual (82.6%) and the majority being sexually active (96.7%). Rates of self-reported lifetime STI testing (70.4%) and intention for future STI screening ('definitely yes' in the next 12months, 39.0%) were also high. The most significant factor associated with future intention for STI testing is the Sexual Experience and Perception Factor (adjusted odds ratio [AOR]=2.02; 95%CI 1.76-2.32; P<0.001), followed by the Sexual Beliefs and Attitudes Factor (AOR=1.14; 95% CI 1.01-1.30; P<0.05). Conclusions The NSW state-wide DTT campaign has largely reached sexually active youth who are attentive to sexual health promotion messages and contributed to enhanced STI screening in a fun and peer-supportive environment.

3.
Sex Health ; 18(5): 432-435, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34789362

RESUMO

Background Rising demand for sexual health services requires publicly funded service providers to ensure they are seeing members of priority populations. Sydney Sexual Health Centre in New South Wales, Australia developed an innovative online triage tool called 'Am I OK?' to support this goal. Methods This paper outlines the findings of a review that examined the use of the triage tool using retrospective cross-sectional analysis of 2017 data. Results The tool has achieved its purpose in ensuring that non-priority populations are referred to other services, consequently saving a significant amount (approximately 6months equivalent) of phone triage nurse time. Conclusion More work may need to be done to ensure that the tool is not creating a barrier for priority populations wishing to access the service.

4.
Am J Cancer Res ; 11(10): 4884-4899, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34765298

RESUMO

Herceptin-resistant tumor relapse remains a major clinical issue responsible for the poor prognosis of HER2+ breast cancer. Understanding the underlying mechanisms and finding a therapeutic solution are of paramount urgency to improve the patient management. Here we report that anticancer redox active cerium oxide nanoparticles (CONPs) can potently sensitize the cancer cells to the cytotoxicity of Herceptin. By comparing between Herceptin-sensitive and Herceptin-resistant human breast cancer cell lines under normoxic as well as hypoxic culture conditions, we found that in the presence of CONPs, Herceptin can kill the Herceptin-resistant cells equally effectively as it kills the Herceptin-sensitive cells under the hypoxic, but not normoxic, culture conditions by inhibiting the cell viability, survival and proliferation. Signaling analysis reveals that under the normoxic conditions, the levels of hypoxia induced factor 1α as well as vascular endothelial growth factor are higher in the Herceptin-resistant cells than that in the Herceptin-sensitive cells and are strongly induced once the culture is switched to the hypoxic conditions, which can be potently suppressed by CONPs. Treatment with CONPs plus Herceptin significantly slows down the primary tumor growth and lung metastasis of the Herceptin-resistant cells in a xenograft mouse model of orthotopic breast cancer through inhibiting the cell proliferation and survival as well as tumor angiogenesis. These results shed new lights on the mechanisms underlying the Herceptin resistance of the HER2+ breast cancer and provide insights into introducing CONPs-like agents to Herceptin-based therapy to improve treatment outcomes.

5.
Cell Chem Biol ; 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-34478637

RESUMO

2'3'-cyclic GMP-AMP (2'3'-cGAMP), generated by cyclic GMP-AMP synthase (cGAS) under activation by cytosolic DNA, has a vital role in innate immune response via its receptor protein stimulator of interferon genes (STING) to fight viral infections and tumors. In order to have a complete understanding of biological functions of 2'3'-cGAMP, it is important to find out whether 2'3'-cGAMP has other unrevealed binding proteins present in mammalian cells and executes unknown functions. Here we report the 2'3'-cGAMP-based photoaffinity probes that capture and isolate 2'3'-cGAMP-binding proteins. These probes enable the identification of some potential 2'3'-cGAMP-binding proteins from HeLa cells. EF1A1, an essential protein regulating protein synthesis, is further validated to associate with 2'3'-cGAMP in vitro and in cells to impede protein synthesis. Thus, our studies provide a powerful approach to enable identification of the 2'3'-cGAMP interactome, discover unknown functions of 2'3'-cGAMP, and understand its physiological/pathological roles in tumor immunity and immune-related diseases.

6.
PeerJ Comput Sci ; 7: e688, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34497874

RESUMO

Background: Rumor detection is a popular research topic in natural language processing and data mining. Since the outbreak of COVID-19, related rumors have been widely posted and spread on online social media, which have seriously affected people's daily lives, national economy, social stability, etc. It is both theoretically and practically essential to detect and refute COVID-19 rumors fast and effectively. As COVID-19 was an emergent event that was outbreaking drastically, the related rumor instances were very scarce and distinct at its early stage. This makes the detection task a typical few-shot learning problem. However, traditional rumor detection techniques focused on detecting existed events with enough training instances, so that they fail to detect emergent events such as COVID-19. Therefore, developing a new few-shot rumor detection framework has become critical and emergent to prevent outbreaking rumors at early stages. Methods: This article focuses on few-shot rumor detection, especially for detecting COVID-19 rumors from Sina Weibo with only a minimal number of labeled instances. We contribute a Sina Weibo COVID-19 rumor dataset for few-shot rumor detection and propose a few-shot learning-based multi-modality fusion model for few-shot rumor detection. A full microblog consists of the source post and corresponding comments, which are considered as two modalities and fused with the meta-learning methods. Results: Experiments of few-shot rumor detection on the collected Weibo dataset and the PHEME public dataset have shown significant improvement and generality of the proposed model.

7.
J Interv Cardiol ; 2021: 5662697, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34456640

RESUMO

Purpose: The objective of this investigation was to study the early efficacy of in situ fenestration with triple chimney technique for high-risk type A aortic dissection patients. Methods: This study included 24 patients who were treated by in situ fenestration with TCT for high-risk TAAD between January 2018 and December 2019. Multiple comorbidities or preoperative critical conditions rendered patients ineligible for open surgery, but all patients that were evaluated and considered had to undergo operation. By analyzing the regular follow-up data, the early postoperative efficacy of the patients was evaluated. Results: The average age of the 24 patients was 65.4 ± 9.3 years. The success rate of the operation was 100%, as all the patients were discharged successfully. There were no serious neurological complications or persistent endoleakage. The mean follow-up time was 21.4 ± 6.9 months. The patency rate of all branching stents was 100%, with no stent displacement, stenosis, or blockage observed. While none presented with type I endoleakage, one patient (4.2%) presented asymptomatic type II endoleakage around the left subclavian artery stent. Currently, 23 of the 24 patients remain alive. Conclusion: Initial results are encouraging with TCT for high-risk TAAD. However, due to its high selectivity and potential complexity related to surgical risks, the mid- and long-term efficacy of this technique remains unknown. For patients who are eligible for open heart surgery, we still recommend it be performed.


Assuntos
Aneurisma Dissecante , Aneurisma da Aorta Torácica , Implante de Prótese Vascular , Procedimentos Endovasculares , Idoso , Aneurisma Dissecante/diagnóstico por imagem , Aneurisma Dissecante/cirurgia , Aneurisma da Aorta Torácica/diagnóstico por imagem , Aneurisma da Aorta Torácica/cirurgia , Prótese Vascular , Implante de Prótese Vascular/efeitos adversos , Humanos , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Stents , Fatores de Tempo , Resultado do Tratamento
8.
World J Clin Cases ; 9(16): 4046-4051, 2021 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-34141765

RESUMO

BACKGROUND: Previous studies reported that most of the intracranial dermoid cyst ruptures were spontaneous, and only a few were traumatic, with asymptomatic much rarer than the symptomatic ruptures. Hence, how to deal with the asymptomatic traumatic rupture of intracranial dermoid cyst remains a challenge in the clinic. CASE SUMMARY: A 59-year-old man was accidentally diagnosed with intracranial dermoid cyst through a cranial computed tomography (CT) scan due to a car accident. A mixed-density lesion with fat and a calcified margin was observed in the midline of the posterior fossa, accompanied with lipid droplet drifts in brain sulci, fissures, cisterns, and ventricles. After 1 wk of conservative observation, no change was observed on the updated cranial CT scan. After 2 wk of conservative observation, magnetic resonance imaging examination confirmed that the lesion was a traumatic rupture of a posterior fossa dermoid cyst with lipid droplet drifts. As the patient exhibited no adverse symptoms throughout the 2 wk, a 6-mo follow-up visit was arranged for him instead of aggressive treatment. Nonetheless, the patient did not show any abnormal neurological symptoms in the 6 mo of follow-up visits. CONCLUSION: Asymptomatic traumatic rupture of intracranial dermoid cyst could be just followed or treated conservatively rather than treated aggressively.

9.
Sci Prog ; 104(2): 368504211026284, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34143701

RESUMO

Aiming at the problem of bus voltage control in DC microgrid, a dynamic compensation control strategy based on a residual generator is designed to complete the voltage compensation of DC-DC converter. Firstly, based on the DC microgrid system architecture, the bus voltage fluctuations are analyzed theoretically, and then the DC-DC converter state-space mathematical models of the DC microgrid system are established to obtain the input-output relationship of the control system. Based on the theory of double coprime decomposition and Youla parameterization stable controller, the proposed control architecture based on the residual generator is obtained, and the output value generated by the current disturbance is compensated in reverse by applying model matching theory. The voltage loop compensation controller Q(s) is obtained by the linear matrix inequality method (LMI), and the current loop compensation controller H(s) is designed according to the dynamic structure diagram of the DC-DC converter. Hardware-in-the-loop simulation (HILS) results show that the architecture can improve the dynamic performance of the DC-DC converter without changing the original system structure parameters, and suppress the DC bus voltage fluctuations caused by load switching, power fluctuations, and AC-side load imbalances, and enhance the robustness of the system.

10.
Am J Cancer Res ; 11(5): 2188-2201, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34094677

RESUMO

Metastasis and drug resistance are intertwined processes that are responsible for the vast majority of patient deaths from breast cancer. The underlying mechanisms remain incompletely understood. We previously demonstrated that KLF8 activates CXCR4 transcription in metastatic breast cancer. Here, we report a novel role of KLF8-CXCR4 signaling for converting single organ metastasis into multiple organ metastasis associated with chemotherapeutic resistance. We show that KLF8 expression in metastatic breast cancer cells can be over-induced by chemotherapeutic drugs. Analysis of data from large-cohorts of patients indicates that post-chemotherapy there is a close correlation between the aberrant high levels of KLF8 and CXCR4 and that this correlation is well associated with drug resistance, metastasis, and poor prognosis. To mimic their aberrant high levels, we overexpressed KLF8 or CXCR4 in a human breast cancer cell line known to metastasize only to the lungs after intravenous injection in nude mice. As expected, these cells become more resistant to chemotherapeutic drugs. Surprisingly, these KLF8 or CXCR4 overexpressing cells, even implanted orthotopically, metastasized extensively to multiple organs particularly the CXCL12-rich organs. Tube formation assay, Ki67 staining and Western blotting revealed that KLF8 or CXCR4 overexpression enhanced angiogenesis involving increased expression and secretion of VEGF protein. We also found that KLF8 or CXCR4 overexpression strongly enhanced formation of filopodium-like protrusions and proliferation via CXCR4 stimulation in a 3D culture model mimicking the colonization step of metastasis. Taken together, these results suggest that the chemo-induction of KLF8 upregulation be critical for drug resistance and systemic metastasis through enhanced tumor angiogenesis and colonization via CXCR4 over-activation and that KLF8-CXCR4 signaling axis may be a new therapeutic target for drug-resistant breast cancer metastasis.

11.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(3): 865-868, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34105485

RESUMO

OBJECTIVE: To understand the genotypes and distribution characteristics of thalassemia in Baise, Guangxi Zhuang Autonomous Region, to provide references for the prevention and diagnosis of thalassemia in the region and improve the quality of eugenics. METHODS: 3 482 pregnant women and their spouses from January 2019 to August 2019 in Baise Maternal and Child Health Hospital for prenatal genetic diagnosis were selected, α, ß- thalassemia genes were detected by Gap-PCR, PCR and DNA reverse dot hybridization, cases carrying thalassemia gene were confirmed and statistical analyzed. RESULTS: 2 260 samples (64.90%) carrying thalassemia gene were found, among which 1 459 cases (64.56%) were diagnosed as α- thalassemia, 617 cases (27.30%) as ß- thalassemia, 184 cases (8.14%) as α complex ß- thalassemia. Among 1 459 α- thalassemia genes, --SEA /αα(637 cases, 43.66%), -α3.7 /αα (306 cases, 20.97%), -αCS /αα(143 cases, 9.80%), -α4.2 /αα(124 cases, 8.50%) and -αWS /αα(77 cases, 5.27%) were the most common, while among 617 ß- thalassemia genes, CD17 (229 cases, 37.12%), CD41-42 (213 cases, 34.52%), IVS-I-1 (41 cases, 6.65%), ßE (38 cases, 6.16%) and CD71-72 (34 cases, 5.51%) were the most common. And --SEA /αα/ CD17 (24 cases, 13.04%), -α4.2 /αα/ CD17 (13 cases, 7.07%), -α3.7 /αα/ CD41-42 (12 cases, 6.52% ) and --SEA /αα/ CD41-42 (12 cases, 6.52%) were mainly found in 184 cases of α complex ß - thalassemia. CONCLUSION: Genotyes of thalassemia in Baise, Guangxi Zhuang Autonomous Region are complex and diverse. The prenatal screening and diagnosis of thalassemia in the region should be strengthened in accordance with the characteristics of genetypes in the region, in order to reduce birth defects and improve eugenics quality.


Assuntos
Talassemia alfa , Talassemia beta , Criança , China , Feminino , Genótipo , Humanos , Mutação , Gravidez , Diagnóstico Pré-Natal , Talassemia alfa/diagnóstico , Talassemia alfa/epidemiologia , Talassemia alfa/genética , Talassemia beta/diagnóstico , Talassemia beta/genética
12.
J Agric Food Chem ; 69(23): 6710-6719, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34080852

RESUMO

Panax quinquefolius is one of the most recognized ginseng species. In this study, lipidome and metabolome extraction methods for P. quinquefolius were optimized with methanol/methyl-tert-butyl ether/water (0.3 mg/1 µL/6 µL/8 µL). A total of 497 metabolites were identified, including 365 lipids and 76 ginsenosides. Comprehensive lipidome profiling was first performed for P. quinquefolius, in which 32.6% glycerophospholipids, 39.5% glycerolipids, 9.3% sphingolipids, 3.3% sterol lipids, and 15.3% fatty acyls were identified. Orthogonal partial least squares discrimination analysis (OPLS-DA) showed obvious metabolomic differences in two growing regions of China. In the northern growing region, the ratio of bilayer- to nonbilayer-forming membrane lipids (PCs/PEs, DGDGs/MGDGs), the degree of unsaturation of acyl chains in galactolipids, and the content of membrane glycerophospholipids were increased. In the eastern growing region, the synthesis of storage lipids, ceramides, and fatty acyls was increased, and secondary metabolism was enhanced with 24 differential ginsenosides found. The investigation deepens the understanding of metabolic regulation mechanisms of P. quinquefolius.


Assuntos
Ginsenosídeos , Panax , China , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Ginsenosídeos/análise , Lipidômica , Espectrometria de Massas , Metaboloma
13.
Redox Biol ; 43: 101970, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33887608

RESUMO

BACKGROUND: Chronic gastroesophageal reflux disease (GERD) is a major risk factor for the development of metaplastic Barrett's esophagus (BE) and its progression to esophageal adenocarcinoma (EAC). Uncontrolled accumulation of reactive oxygen species (ROS) in response to acidic bile salts (ABS) in reflux conditions can be lethal to cells. In this study, we investigated the role of APE1/REF1 in regulating nuclear erythroid factor-like 2 (NRF2), the master antioxidant transcription factor, in response to reflux conditions. RESULTS: We found that APE1 protein was critical for protecting against cellular ROS levels, oxidative DNA damage, double strand DNA breaks, and cell death in response to conditions that mimic reflux. Analysis of cell lines and de-identified tissues from patients with EAC demonstrated overexpression of both APE1 and NRF2 in EAC cells, as compared to non-neoplastic esophageal cells. Using reflux conditions, we detected concordant and prolonged increases of APE1 and NRF2 protein levels for several hours, following transient short exposure to ABS (20 min). NRF2 transcription activity, as measured by ARE luciferase reporter, and expression of its target genes (HO-1 and TRXND1) were similarly increased in response to ABS. Using genetic knockdown of APE1, we found that APE1 was required for the increase in NRF2 protein stability, nuclear localization, and transcription activation in EAC. Using knockdown of APE1 with reconstitution of wild-type and a redox-deficient mutant (C65A) of APE1, as well as pharmacologic APE1 redox inhibitor (E3330), we demonstrated that APE1 regulated NRF2 in a redox-dependent manner. Mechanistically, we found that APE1 is required for phosphorylation and inactivation of GSK-3ß, an important player in the NRF2 degradation pathway. CONCLUSION: APE1 redox function was required for ABS-induced activation of NRF2 by regulating phosphorylation and inactivation of GSK-3ß. The APE1-NRF2 network played a critical role in protecting esophageal cells against ROS and promoting cell survival under oxidative reflux conditions.


Assuntos
Adenocarcinoma , Neoplasias Esofágicas , DNA Liase (Sítios Apurínicos ou Apirimidínicos) , Glicogênio Sintase Quinase 3 beta , Humanos , Fator 2 Relacionado a NF-E2/metabolismo , Oxirredução
14.
Huan Jing Ke Xue ; 42(5): 2213-2222, 2021 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-33884790

RESUMO

Urban water is a significant part of the urban ecosystem. Therefore, a comprehensive evaluation method of the water environment was proposed based on domestic high-resolution images. The relationships between the spectral characteristics and water quality parameters of urban water were analyzed based on sampling in Nanjing, Wuxi, Changzhou, and Yangzhou from 2017 to 2019. An index named the U-FUI (urban Forel-Ule index) suitable for urban water based on GF-2 images was proposed to achieve the classification of urban water on the basis of the international standard chroma conversion model and the Forel-Ule index. Independent verification data showed that the recognition accuracy of the classification model could reach 72%. The results indicated that urban water can be classified into six classes from Ⅰ to Ⅵ, which represent water colors of blue, light green, dark green, yellow, yellowish brown, and dark grey, respectively, according to the U-FUI. Among them, the water quality of U-FUI Ⅰ water is good, but is rarely distributed in urban water. The concentrations of chlorophyll-a in U-FUI Ⅱ-Ⅲ water are higher than those of the other classes; the concentrations of total suspended solids, particularly inorganic suspended solids, of U-FUI Ⅳ-Ⅴ water are higher than those of the other classes; and the water quality of U-FUI Ⅵ water is poor and the water quality parameters are different from those of the other classes. Meanwhile, the method was successfully applied to the GF-2 image of Nanjing on April 9, 2018. The results showed that the urban water in Nanjing is mainly composed of U-FUI Ⅱ-Ⅳ water, whereas the distribution of U-FUI Ⅰ, Ⅴ, and Ⅵ water is lower in the city. The spatial distribution characteristics were consistent with the results of in-situ sampling in the same period.

15.
Acta Pharmacol Sin ; 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33686245

RESUMO

Aristolochic acid I (AAI) is a well-known nephrotoxic carcinogen, which is currently reported to be also associated with hepatocellular carcinoma (HCC). Whether AAI is a direct hepatocarcinogen remains controversial. In this study we investigated the association between AAI exposure and HCC in adult rats using a sensitive rat liver bioassay with several cofactors. Formation of glutathione S-transferase placental form-positive (GST-P+) foci was used as the marker for preneoplastic lesions/clonal expansion. We first conducted a medium-term (8 weeks) study to investigate whether AAI had any tumor-initiating or -promoting activity. Then a long-term (52 weeks) study was conducted to determine whether AAI can directly induce HCC. We showed that oral administration of single dose of AAI (20, 50, or 100 mg/kg) in combination with partial hepatectomy (PH) to stimulate liver proliferation did not induce typical GST-P+ foci in liver. In the 8-week study, only high dose of AAI (10 mg · kg-1 · d-1, 5 days a week for 6 weeks) in combination with PH significantly increased the number and area of GST-P+ foci initiated by diethylnitrosamine (DEN) in liver. Similarly, only high dose of AAI (10 mg· kg-1· d-1, 5 days a week for 52 weeks) in combination with PH significantly increased the number and area of hepatic GST-P+ foci in the 52-week study. No any nodules or HCC were observed in liver of any AAI-treated groups. In contrast, long-term administration of AAI (0.1, 1, 10 mg· kg-1· d-1) time- and dose-dependently caused death due to the occurrence of cancers in the forestomach, intestine, and/or kidney. Besides, AAI-DNA adducts accumulated in the forestomach, kidney, and liver in a time- and dose-dependent manner. Taken together, AAI promotes clonal expansion only in the high-dose group but did not induce any nodules or HCC in liver of adult rats till their deaths caused by cancers developed in the forestomach, intestine, and/or kidney. Findings from our animal studies will pave the way for further large-scale epidemiological investigation of the associations between AA and HCC.

16.
Diabetes Metab Syndr Obes ; 14: 329-344, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33519221

RESUMO

Diabetic kidney disease (DKD), as the main complication of diabetes mellitus, is the primary cause of the end-stage renal disease (ESRD) and the most common chronic kidney disease. Overall, 30-40% of patients with type 1 and type 2 diabetes eventually develop DKD. Although some diabetes patients have intensified glycemic control, they still develop diabetic kidney disease. Current treatment methods can alleviate but do not markedly halt disease development, resulting in renal failure and severe complications, even contributing to elevated morbidity and mortality rates. DKD is a disease with interactions of genes and the environment. Emerging evidence indicates that DKD-associated key genes are also regulated by the epigenetic mechanism. Recently, increasing researches involving cells and experimental animals demonstrated that histone post-translational modifications can mediate gene expression, which correlated with diabetic kidney disease. Novel therapeutic strategies for epigenetic events could be beneficial for the early detection and treatment of DKD to prevent it from developing into end-stage renal disease (ESRD). In this review, we discuss prior findings in the field of histone modifications in DKD, especially histone acetylation and histone methylation. We then focus on recent developments in histone acetylation and methylation involved in the pathogenesis of DKD.

17.
Environ Sci Pollut Res Int ; 28(21): 27067-27083, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33501583

RESUMO

As the remote sensing technology develops, there are increasingly more kinds of remote sensing images available from different sensors. High-resolution remote sensing images are widely used in the detection of land cover/land change due to their plenty of characteristics of a specific feature in terms of spectrum, shape, and texture. Current studies regarding cultivated land resources that are the material basis for the human beings to survive and develop focus on the method to accurately obtain the quantity of cultivated land in a region and understand the conditions and the trend of change of the cultivated land. Pixel-based method and object-oriented method are the main methods to extract cultivated land in remote sensing field. Pixel-based method ignores high-level image information, while object-oriented method takes the image spot after image segmentation as the basic unit of information extraction, which can make full use of spectral features, spatial features, semantic features, and contextual features. Image segmentation is a key step of object-oriented method; the core problem is how to obtain the optimal segmentation scale. Traditional methods for determining the optimal segmentation scale of features (such as the homogeneity-heterogeneity method, the maximum area method, and the mean variance method), in which only the spectral and geometrical characteristics are considered, while the textural characteristics are neglected. Based on this, the Quickbird and unmanned aerial vehicle (UAV) images obtained in Xiyu Village, Pengzhou City, Sichuan Province, China, were selected as experimental objects, and the texture mean and spectral grayscale mean method (MANC method based on GLCM), which comprehensively considered the spectrum, shape, and texture features, was proposed to calculate the optimal segmentation scale of cultivated land in the study area. The error segment index (ESI) and centroids distance index (CDI) were adopted to evaluate image segmentation quality based on the method of area and position differences. The experimental results show that the MANC method based on GLCM can obtain higher segmentation precision than the traditional methods, and the segmentation results are in good agreement with the cultivated land boundary obtained by visual interpretation.


Assuntos
Tecnologia de Sensoriamento Remoto , China , Humanos
18.
Huan Jing Ke Xue ; 41(11): 5060-5072, 2020 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124249

RESUMO

Remote sensing monitoring of black-odor water is an important method for understanding the current status of urban water quality, and comprehensively evaluating the effect of urban water environment treatment. A total of 171 samples were collected in Nanjing, Changzhou, Wuxi, and Yangzhou cities and water quality parameters and optical parameters were measured simultaneously. Based on the analysis of the water color and optical characteristics of the black-odor water and non-black-odor water (denoted as general water), a decision tree was constructed to identify the severe, mild black-odor water, and general water as green and yellow water. The results found that:①According to the water color, the water bodies can be divided into six types. Among them, type 1 to 4 water bodies are black-odor water, which are gray black, dark gray, gray, and light gray water, respectively, and type 5 and 6 water bodies are general water, which are green and yellow water, respectively; ②Type 1 water body contains high contents of non-pigmented particulate matter and colored dissolved organic matter(CDOM), however, the absorption of pigmented particulate matter is not dominant. Type 2 and 5 water bodies are dominated by pigmented particulate matter. Type 3, 4, and 6 water bodies are dominated by non-pigmented particulate matter; ③After water color classification, and according to the differences of the reflection spectrums of the six types of water bodies, the difference of black-odorous water index (DBWI), green-red-nir area water index (G-R-NIR AWI), the green band reflectance and the normalized difference black-odorous water index (NDBWI) were used to construct a decision tree to identify the severe, mild black-odor water, and general water; ④The decision tree was applied to the PlanetScope satellite image of Yangzhou City on April 9, 2019, and 10 synchronous sampling points were used for verification. The overall recognition accuracy reached 80.00%, and the K value reached 0.67. The urban water classification model, after water color classification, can be applied to other similar water bodies, and provides a technical method for the supervision of black-odor water bodies.


Assuntos
Tecnologia de Sensoriamento Remoto , Água , Cidades , Árvores de Decisões , Monitoramento Ambiental , Odorantes
19.
Cancers (Basel) ; 12(9)2020 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-32927604

RESUMO

The incidence of esophageal adenocarcinoma (EAC) has been rising dramatically in the past few decades in the United States and Western world. The N-myc downregulated gene 4 (NDRG4) belongs to the human NDRG family. In this study, we aimed to identify the expression levels, regulation, and functions of NDRG4 in EAC. Using an integrative epigenetic approach, we identified genes showing significant downregulation in EAC and displaying upregulation after 5-Aza-deoxycitidine. Among these genes, likely to be regulated by DNA methylation, NDRG4 was among the top 10 candidate genes. Analyses of TCGA (The Cancer Genome Atlas) and GEO (Gene Expression Omnibus) data sets and EAC tissue samples demonstrated that NDRG4 was significantly downregulated in EAC (p < 0.05). Using Pyrosequencing technology for quantification of DNA methylation, we detected that NDRG4 promoter methylation level was significantly higher in EAC tissue samples, as compared to normal esophagus samples (p < 0.01). A strong inverse correlation between NDRG4 methylation and its gene expression levels (r = -0.4, p < 0.01) was observed. Treatment with 5-Aza restored the NDRG4 expression, confirming that hypermethylation is a driving force for NDRG4 silencing in EAC. Pathway and gene set enrichment analyses of TCGA data suggested that NDRG4 is strongly associated with genes related to cell cycle regulation. Western blotting analysis showed significant downregulation of Cyclin D1, CDK4 and CDK6 in EAC cells after overexpression of NDRG4. Functionally, we found that the reconstitution of NDRG4 resulted in a significant reduction in tumor cell growth in two-dimensional (2D) and three-dimensional (3D) organotypic culture models and inhibited tumor cell proliferation as indicated by the EdU (5-ethynyl-2'-deoxyuridine) proliferation assay.

20.
Cancer Lett ; 491: 87-96, 2020 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-32735911

RESUMO

Infection with Helicobacter pylori (H. pylori) is the main risk factor for gastric carcinogenesis. In this study, we investigated the expression, molecular functions, and downstream effectors of miR490-3p in gastric cancer. We used in vitro and in vivo models to investigate the role of H. pylori in regulating miR490-3p, DARPP-32-dependent functions, and therapeutic resistance. Human and mouse neoplastic gastric lesions demonstrated a negative correlation between DARPP-32 and miR490-3p expression (R = -0.58, P < 0.01). This was also detected following infection with H. pylori (R = -0.66, P < 0.01). Molecular assays confirmed DARPP-32 as a direct target of miR490-3p. CHRM2, the host gene of miR490-3p, was hypermethylated and downregulated in neoplastic gastric tissues (P < 0.05). H. pylori induced methylation and downregulation of CHRM2 and miR490-3p. Functionally, the reconstitution of miR490-3p sensitized cancer cells to gefitinib by inactivating DRAPP-32-dependent AKT and STAT3 pathways. Patients with low miR490-3p or high DARPP-32 expression had decreased overall survival (P < 0.05). Hypermethylation-mediated silencing of CHRM2 and miR490-3p by H. pylori increased DARPP-32 expression. Downregulation of miR490-3p in gastric cancer plays a role in gefitinib response by inducing DARPP-32-mediated activation of PI3K/AKT, STAT3 signaling pathways.


Assuntos
Fosfoproteína 32 Regulada por cAMP e Dopamina/fisiologia , Gefitinibe/uso terapêutico , Helicobacter pylori/patogenicidade , MicroRNAs/fisiologia , Neoplasias Gástricas/tratamento farmacológico , Animais , Linhagem Celular Tumoral , Metilação de DNA , Resistencia a Medicamentos Antineoplásicos , Infecções por Helicobacter/complicações , Humanos , Camundongos , Camundongos Endogâmicos C57BL , MicroRNAs/antagonistas & inibidores , Receptor Muscarínico M2/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/microbiologia
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