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1.
Food Chem ; 400: 133998, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36055141

RESUMO

Colostrum is essential for immune system development and has a protective role for infants in early life. However, the lipid compositions of human and ewe colostra have not been characterized. We hypothesized that lipidomics can be used to compare lipids in two mammalian colostra. Herein, 1004 lipids assigned to 26 subclasses were identified in both human and ewe colostra using a quantitative lipidomics approach. In total, 173 significantly different lipids (SDLs) were investigated (variable importance in projection > 1.1, fold change (FC) ≥ 2 or ≤0.5, and P < 0.0001). Four potential lipid biomarkers, namely, DG (19:0/18:0), TG (10:0/15:0/16:0), FFA (22:0), and TG (18:1/24:1/18:2), were selected from the 173 SDLs based on FC values. These different lipids were involved in 44 metabolic pathways, of which sphingolipid metabolism and glycerophospholipid metabolism were the major pathways. Our results improve the understanding of the differences between human and ewe colostra lipids.


Assuntos
Colostro , Lipidômica , Animais , Biomarcadores/metabolismo , Colostro/metabolismo , Feminino , Glicerofosfolipídeos/metabolismo , Humanos , Metabolismo dos Lipídeos , Lipídeos , Mamíferos , Gravidez , Ovinos , Esfingolipídeos
2.
Eur J Med Chem ; 243: 114729, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36084535

RESUMO

Butyrylcholinesterase (BChE) is recently regarded as a biomarker in progressed Alzheimer's disease (AD), the development of selective BChE inhibitors has attracted a great deal of interest and may be a viable therapeutic strategy for AD. Previously, an aromatic tertiary amine derivative (S17-1001) was screened and validated as a selective BChE inhibitor. Structured-based molecular modification guided the synthesis of 43 analogs. Biological test of cholinesterase inhibition, in vitro blood brain barrier permeation assay, neurotoxicity assay and neuroprotective effects assay indicated two optimal compounds 17c and 19c. Both compounds showed selective BChE inhibitory (hBChE < 20 nM, eeAChE > 10 µM), good BBB permeation and primary cell safety. Besides, 17c can dose-response protect cell from Aß1-42 induced damage. It also demonstrated that 17c and 19c were able to restore cognitive impairment in vivo test. These data suggest that 17c and 19c represent promising candidate for follow-up in the drug-discovery process against AD.

3.
Phys Chem Chem Phys ; 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36129075

RESUMO

Selective methanol synthesis via CO2 hydrogenation has been thoroughly investigated over defective In-doped m-ZrO2 using density functional theory (DFT). Three types of oxygen vacancies (Ovs) generated either at the top layer (O1_v and O4_v) or at the subsurface layer (O2_v) are chosen as surface models due to low Ov formation energy. Surface morphology reveals that O1_v has smaller oxygen vacancy size than O4_v. Compared with perfect In@m-ZrO2, indium on both O1_v and O4_v is partially reduced, whereas the Bader charge of In on O2_v remains almost the same. Our calculations show that CO2 is moderate in adsorption energy (∼-0.8 eV) for all investigated surface models, which facilitates the formate pathway for both O1_v and O4_v. O2_v is not directly involved in CO2 methanolization but could readily transform into O1_v once CO2/H2 feed gas is introduced. Based on the results, the synthesis of methanol from CO2 hydrogenation turns out to exhibit conspicuous vacancy size-dependency for both O1_v and O4_v. The reaction mechanism for small-sized O1_v is controlled by both the vacancy size effect and surface reducibility effect. Thus, H2COO* favors direct C-O bond cleavage (c-mechanism) before further hydrogenation to methanol, which is similar to the defective In2O3. The vacancy size effect is more competitive than the surface reducibility effect for large-sized O4_v. Therefore, H2COO* prefers protonation to H2COOH before C-O bond cleavage (p-mechanism) which is similar to the ZnO-ZrO2 solid solution. Furthermore, we also determined that stable-CH3O*, which is too stable to be hydrogenated, originates from the O1_v surface. In contrast, CH3O* with similar configuration is allowed to be further converted to methanol on O4_v. Overall, our findings offer a new perspective towards how reaction mechanisms are determined by the size of oxygen vacancies.

4.
World J Emerg Med ; 13(5): 367-372, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119770

RESUMO

BACKGROUND: Sepsis is a common cause of death in emergency departments and sepsis-associated encephalopathy (SAE) is a major complication. Rosuvastatin may play a neuroprotective role due to its protective effects on the vascular endothelium and its anti-inflammatory functions. Our study aimed to explore the potential protective function of rosuvastatin against SAE. METHODS: Sepsis patients without any neurological dysfunction on admission were prospectively enrolled in the "Rosuvastatin for Sepsis-Associated Acute Respiratory Distress Syndrome" study (SAILS trial, ClinicalTrials.gov number: NCT00979121). Patients were divided into rosuvastatin and placebo groups. This is a secondary analysis of the SAILS dataset. Baseline characteristics, therapy outcomes, and adverse drug events were compared between groups. RESULTS: A total of 86 patients were eligible for our study. Of these patients, 51 were treated with rosuvastatin. There were significantly fewer cases of SAE in the rosuvastatin group than in the placebo group (32.1% vs. 57.1%, P=0.028). However, creatine kinase levels were significantly higher in the rosuvastatin group than in the placebo group (233 [22-689] U/L vs. 79 [12-206] U/L, P=0.034). CONCLUSION: Rosuvastatin appears to have a protective role against SAE but may result in a higher incidence of adverse events.

5.
Front Immunol ; 13: 987841, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36110863

RESUMO

Objective: To explore the clinical features and prognoses of dermatomyositis (DM) associated with a double-positive anti-MDA5 and anti-aminoacyl-tRNA synthetase (anti-ARS) antibody presentation. Methods: We retrospectively analyzed 1280 consecutive patients with idiopathic inflammatory myopathy (IIM). Individuals with anti-MDA5 and anti-ARS antibodies (anti-MDA5+/ARS+) were compared to anti-MDA5-/ARS+ and anti-MDA5+/ARS- control individuals based on clinical, pulmonary radiological characteristics, treatment, and follow-up information. Results: Six individuals (0.47%) presented with anti-MDA5+/ARS+; of these, 2 (33.3%) were anti-PL-12+, 2 (33.3%) were anti-Jo-1+, 1 (16.7%) was anti-EJ+, and 1 (16.7%) was anti-PL-7+. Hallmark cutaneous manifestations, including Gottron's sign (100%), heliotrope rash (50%), mechanic's hand (66.7%), and skin ulcers (16.7%) were common. Anti-MDA5+/ARS+ patients tended to have higher ferritin levels (p = 0.038) than anti-MDA5-/ARS+ group, and higher CD4+ T-cell counts (p = 0.032) compared to the anti-MDA5+/ARS- group. Radiologically, NSIP with OP overlap was predominant (60%). Consolidation (60%), ground-glass attenuation (GGA) (80%), traction bronchiectasis (80%), and intralobular reticulation (100%) were common in anti-MDA5+/ARS+ individuals. All were diagnosed with ILD and 50% were categorized as RPILD. All patients received glucocorticoids combined with one or more immunosuppressants. Most (83.3%) had a good prognosis following treatment, but there was no difference in the survival rate between the three subgroups. Conclusion: Presentation with anti-MDA5+/ARS+ DM was rare. The clinical and radiological characteristics of anti-MDA5+/ARS+ DM combined the features of anti-MDA5+ and anti-ARS+ individuals. Individuals with anti-MDA5+/ARS+ antibodies may respond well to glucocorticoid therapy; glucocorticoids combined with one or more immunosuppressants may be considered a basic treatment approach.


Assuntos
Aminoacil-tRNA Sintetases , Dermatomiosite , Doenças Pulmonares Intersticiais , Autoanticorpos , Dermatomiosite/diagnóstico , Dermatomiosite/tratamento farmacológico , Ferritinas , Glucocorticoides , Humanos , Imunossupressores , Doenças Pulmonares Intersticiais/complicações , Doenças Pulmonares Intersticiais/diagnóstico , Doenças Pulmonares Intersticiais/tratamento farmacológico , Prognóstico , Estudos Retrospectivos
6.
BMC Med Imaging ; 22(1): 157, 2022 09 03.
Artigo em Inglês | MEDLINE | ID: mdl-36057576

RESUMO

OBJECTIVES: We aimed to investigate the value of performing gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) enhanced magnetic resonance imaging (MRI) radiomics for preoperative prediction of microvascular invasion (MVI) of hepatocellular carcinoma (HCC) based on multiple sequences. METHODS: We randomly allocated 165 patients with HCC who underwent partial hepatectomy to training and validation sets. Stepwise regression and the least absolute shrinkage and selection operator algorithm were used to select significant variables. A clinicoradiological model, radiomics model, and combined model were constructed using multivariate logistic regression. The performance of the models was evaluated, and a nomogram risk-prediction model was built based on the combined model. A concordance index and calibration curve were used to evaluate the discrimination and calibration of the nomogram model. RESULTS: The tumour margin, peritumoural hypointensity, and seven radiomics features were selected to build the combined model. The combined model outperformed the radiomics model and the clinicoradiological model and had the highest sensitivity (90.89%) in the validation set. The areas under the receiver operating characteristic curve were 0.826, 0.755, and 0.708 for the combined, radiomics, and clinicoradiological models, respectively. The nomogram model based on the combined model exhibited good discrimination (concordance index = 0.79) and calibration. CONCLUSIONS: The combined model based on radiomics features of Gd-EOB-DTPA enhanced MRI, tumour margin, and peritumoural hypointensity was valuable for predicting HCC microvascular invasion. The nomogram based on the combined model can intuitively show the probabilities of MVI.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Carcinoma Hepatocelular/cirurgia , Meios de Contraste , Gadolínio DTPA , Humanos , Neoplasias Hepáticas/irrigação sanguínea , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/cirurgia , Imageamento por Ressonância Magnética/métodos
9.
Int J Mol Sci ; 23(17)2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36077485

RESUMO

The metal titanium is often used as a dental implant material, and the elastic modulus of solid titanium implants does not match the biological bone tissue, which can easily produce a stress shielding effect and cause implant failure. In this paper, a three-level gradient porous structure implant was designed, and its mechanical and biological adaptability were studied by finite element simulation analysis. Combined with the comprehensive evaluation of the mechanical and biological properties of implants of various structures, the analysis found that a porous implant with porosity of 59.86% of the gradient was the best structure. The maximum equivalent stress of this structure in the mandible that simulated the oral environment was 154.34 MPa, which was less than half of its theoretical compression yield strength. The strain of the surrounding bone tissue lies in the bone compared with other structures, the proportion of the active state of plastic construction is larger, at 10.51%, and the fretting value of this structure and the bone tissue interface is the smallest, at only 10 µm.


Assuntos
Implantes Dentários , Titânio , Análise de Elementos Finitos , Mandíbula , Porosidade , Estresse Mecânico , Titânio/química
10.
Cells ; 11(17)2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36078104

RESUMO

Obesity is a major risk factor for both metabolic and cardiovascular disease. We reported that, in obese male mice, histone deacetylase 9 (HDAC9) is upregulated in adipose tissues, and global deletion of HDAC9 protected against high fat diet (HFD)-induced obesity and metabolic disease. Here, we investigated the impact of adipocyte-specific HDAC9 gene deletion on diet-induced obesity in male and female mice. The HDAC9 gene expression was increased in adipose tissues of obese male and female mice and HDAC9 expression correlated positively with body mass index in humans. Interestingly, female, but not male, adipocyte-specific HDAC9 KO mice on HFD exhibited reduced body weight and visceral adipose tissue mass, adipocyte hypertrophy, and improved insulin sensitivity, glucose tolerance and adipogenic differentiation gene expression. Furthermore, adipocyte-specific HDAC9 gene deletion in female mice improved metabolic health as assessed by whole body energy expenditure, oxygen consumption, and adaptive thermogenesis. Mechanistically, compared to female mice, HFD-fed male mice exhibited preferential HDAC9 expression in the stromovascular fraction, which may have offset the impact of adipocyte-specific HDAC9 gene deletion in male mice. These results suggest that HDAC9 expressed in adipocytes is detrimental to obesity in female mice and provides novel evidence of sex-related differences in HDAC9 cellular expression and contribution to obesity-related metabolic disease.


Assuntos
Doenças Metabólicas , Obesidade , Tecido Adiposo/metabolismo , Animais , Dieta Hiperlipídica/efeitos adversos , Feminino , Histona Desacetilases , Humanos , Doenças Metabólicas/genética , Camundongos , Camundongos Obesos , Obesidade/metabolismo , Proteínas Repressoras
11.
Annu Int Conf IEEE Eng Med Biol Soc ; 2022: 3043-3046, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-36086158

RESUMO

Ultrasound computed tomography (USCT) is considered to have great potential for breast cancer screening. Compared with the ray based methods, the reconstructed image using full waveform inversion (FWI) methods have higher spatial resolution. However, the results of FWI is difficult to converge to the real value when cycle skipping occurs. In this paper, a cross-correlation full waveform inversion(CC-FWI) is proposed for USCT image reconstruction. In the first stage, the ajoint source is adjusted as the residual of predicted signal and time-shifted measured signal to avoid cycle skipping. In the remaining stage, the FWI with source encoding is employed to accelerate convergence. The simulations are conducted to demonstrate the validity of the proposed algorithm. The root mean squared error (RMSE) of the proposed algorithm is much smaller than that of conventional FWI. The results suggest that CC-FWI is effective in avoiding cycle skipping. Clinical Relevance- Clinical relevance- New imaging modalities of high resolution, safety to examines for early-stage breast cancer imaging are urgently needed for researching and development. Ultra-sound computed tomography (USCT) is supposed to meet the above requirements and it can be potentially deployed in breast scanning.


Assuntos
Neoplasias da Mama , Tomografia Computadorizada por Raios X , Algoritmos , Neoplasias da Mama/diagnóstico por imagem , Feminino , Humanos , Imagens de Fantasmas , Som , Ultrassonografia/métodos
12.
Annu Int Conf IEEE Eng Med Biol Soc ; 2022: 553-556, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-36086307

RESUMO

In minimally invasive interventional surgery, ultrasound imaging is usually used to provide real-time feedback in order to obtain the best diagnostic results or realize treatment plans, so how to accurately obtain the position of the medical biopsy needle is a problem worthy of study. 2D ultrasound simulation images containing the medical biopsy needle are generated, and our images background is from the real breast ultrasound image. Based on the deep learning network, the images containing the medical biopsy needle are used to analyze the effectiveness of different networks for needle localization for the purpose of returning needle positions in non-uniform ultrasound images. The results show that attention U-Net performed best and can accurately reflect the real position of the medical biopsy needle. The IoU and Precision can reach 90.19% and 96.25%, and the Angular Error is 0.40°. Clinical Relevance- Based on the deep network, for 2D ultrasound images containing medical biopsy needle, the localization precision can reach 96.25% and the Angular Error is 0.40°.


Assuntos
Algoritmos , Agulhas , Biópsia por Agulha/métodos , Feminino , Humanos , Ultrassonografia/métodos , Ultrassonografia Mamária
13.
Curr Opin Rheumatol ; 2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36082751

RESUMO

PURPOSE OF REVIEW: This review provides updates regarding biomarker studies that address key clinical unmet needs, which relate to the evaluation of the disease activity in patients with dermatomyositis. RECENT FINDINGS: Increasing evidence supports that the serum levels of dermatomyositis-specific antibodies (DM-MSAs), which include anti-Mi-2, anti-NXP2, anti-MDA5, anti-TNF1-γ, and anti-SAE, are correlated with the disease activity. Moreover, serial measurements of DM-MSA levels may help to predict the disease status. Beyond the MSA, macrophage activation-related biomarker-soluble CD163, CD206, neopterin, and galectin-3/9 are the most currently talked biomarkers for disease activity in dermatomyositis; new circulating T-cell subsets CD4+CXCR5+CCR7loPD-1hi and TIGIT+CD226+ CD4 T cells can potentially harbor biomarkers of disease activity in dermatomyositis. In addition, LDGs and NETs were also shown to be correlated with the disease activities of dermatomyositis. SUMMARY: Promising candidate biomarkers are now available for evaluating disease activity in dermatomyositis. These biomarkers need external validation in other large cohort studies.

14.
Cell Rep ; 40(12): 111361, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36130501

RESUMO

Hematopoietic stem/progenitor cells (HSPCs) are supported and regulated by niche cells in the bone marrow with an important characterization of physiological hypoxia. However, how hypoxia regulates HSPCs is still unclear. Here, we find that meteorin (Metrn) from hypoxic macrophages restrains HSPC mobilization. Hypoxia-induced factor 1α and Yin Yang 1 induce the high expression of Metrn in macrophages, and macrophage-specific Metrn knockout increases HSPC mobilization through modulating HSPC proliferation and migration. Mechanistically, Metrn interacts with its receptor 5-hydroxytryptamine receptor 2b (Htr2b) to regulate the reactive oxygen species levels in HSPCs through targeting phospholipase C signaling. The reactive oxygen species levels are reduced in HSPCs of macrophage-specific Metrn knockout mice with activated phospholipase C signaling. Targeting the Metrn/Htr2b axis could therefore be a potential strategy to improve HSPC mobilization for stem cell-based therapy.


Assuntos
Células da Medula Óssea , Medula Óssea , Animais , Medula Óssea/metabolismo , Células da Medula Óssea/metabolismo , Mobilização de Células-Tronco Hematopoéticas , Células-Tronco Hematopoéticas/metabolismo , Hipóxia/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Espécies Reativas de Oxigênio/metabolismo , Receptores de Serotonina/metabolismo , Fosfolipases Tipo C/metabolismo
15.
Mol Psychiatry ; 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36138127

RESUMO

Social anhedonia, a loss of interest and pleasure in social interactions, is a common symptom of major depression as well as other psychiatric disorders. Depression can occur at any age, but typically emerges in adolescence or early adulthood, which represents a sensitive period for social interaction that is vulnerable to stress. In this study, we evaluated social interaction reward using a conditioned place preference (CPP) paradigm in adolescent male and female mice. Adolescent mice of both sexes exhibited a preference for the social interaction-associated context. Chronic unpredictable stress (CUS) impaired the development of CPP for social interaction, mimicking social anhedonia in depressed adolescents. Conversely, administration of leptin, an adipocyte-derived hormone, enhanced social interaction-induced CPP in non-stressed control mice and reversed social anhedonia in CUS mice. By dissecting the motivational processes of social CPP into social approach and isolation avoidance components, we demonstrated that leptin treatment increased isolation aversion without overt social reward effect. Further mechanistic exploration revealed that leptin stimulated oxytocin gene transcription in the paraventricular nucleus of the hypothalamus, while oxytocin receptor blockade abolished the leptin-induced enhancement of socially-induced CPP. These results establish that chronic unpredictable stress can be used to study social anhedonia in adolescent mice and provide evidence that leptin modulates social motivation possibly via increasing oxytocin synthesis and oxytocin receptor activation.

16.
RSC Adv ; 12(38): 24590-24595, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36128376

RESUMO

Seven undescribed carotane sesquiterpenoids named fusanoids A-G (1-7), along with one known analog (8) and two known sesterterpenes (9 and 10), were isolated from the fermentation broth of the desert endophytic fungi Fusarium sp. HM166. The structures of the compounds, including their absolute configurations, were determined by spectroscopic data, single-crystal X-ray diffraction analysis, and ECD calculations. Compound 10 showed cytotoxic activities against human hepatoma carcinoma cell line (Huh-7) and human breast cell lines (MCF-7 and MDA-MB-231), and compound 2 showed cytotoxic activity against MCF-7, while compounds 4-9 were inactive against all the tested cell lines. Compounds 4 and 10 showed potent inhibitory activities against the IDH1R132h mutant.

17.
Int J Mol Sci ; 23(18)2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36142797

RESUMO

Strigolactones (SLs) are a class of important hormones in the regulation of plant branching. In the model plant Arabidopsis,&nbsp;AtMAX1 encodes a cytochrome P450 protein and is a crucial gene in the strigolactone synthesis pathway. Yet, the regulatory mechanism of MAX1 in the shoot branching of wintersweet (Chimonanthus praecox) remains unclear. Here we identified and isolated three MAX1 homologous genes, namely CpMAX1a, CpMAX1b, and CpMAX1c. Quantitative real-time PCR (qRT-PCR) revealed the expression of CpMAX1a in all tissues, being highest in leaves, whereas CpMAX1b was only expressed in stems, while CpMAX1c was expressed in both roots and stem tips. However, CpMAX1a's expression decreased significantly after decapitation; hence, we verified its gene function. CpMAX1a was located in Arabidopsis chloroplasts. Overexpressing CpMAX1a restored the phenotype of the branching mutant max1-3, and reduced the rosette branch number, but resulted in no significant phenotypic differences from the wild type. Additionally, expression of AtBRC1 was significantly upregulated in transgenic lines, indicating that the CpMAX1a gene has a function similar to the homologous gene of Arabidopsis. In conclusion, our study shows that CpMAX1a plays a conserved role in regulating the branch development of wintersweet. This work provides a molecular and theoretical basis for better understanding the branch development of wintersweet.

18.
J Mol Cell Biol ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36073763

RESUMO

In eukaryotic cells, both alternative splicing and alternative polyadenylation (APA) play essential roles in gene regulation network. U1 small ribonucleoprotein particle (U1 snRNP) is a major component of spliceosome, and U1 snRNP complex can suppress proximal APA sites through crosstalking with 3' end processing factors. However, here we show that both knockdown and overexpression of SNRPA, SNRPC, SNRNP70, and SNRPD2, the U1 snRNP proteins, promote the usage of proximal APA sites in transcriptome level. SNRNP70 can drive the phase transition of PABPN1 from droplet to aggregate, which may reduce the repression effects of PABPN1 on the proximal APA sites. Additionally, SNRNP70 can also promote the proximal APA sites by recruiting CPSF6, suggesting that the function of CPSF6 on APA is related with other RNA binding proteins and cell context-dependent. Consequently, these results reveal that, on the contrary to U1 snRNP complex, the free proteins of U1 snRNP complex can promote proximal APA sites through the interaction with 3' end processing machinery.

19.
J Hazard Mater ; 441: 129875, 2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36067554

RESUMO

Although iron-based metal-organic frameworks (Fe-MOFs) have displayed the photocatalytic activity, there is still abundant room for improving their photocatalytic performance through tuning the structures. In this work, four novel iron-based metal-organic frameworks (Fe-MOFs) were successfully synthesized via ligand modulation for better photocatalytic Cr(VI) reduction, in which MTBDC-TPT-Fe had the highest catalytic activity (MTBDC = 2,5-bis(methylthio)terephthalic acid, TPT = 2,4,6-tri(4-pyridyl)- 1,3,5-triazine). The boosted photocatalytic reduction may be mainly ascribed to the enhanced electron push-pull effect between iron-oxygen clusters and organic ligands. The introduction of -SCH3 groups can enhance the light absorption and donate electrons to iron center under visible-light irradiation, meanwhile the separation and transfer of photogenerated charge carriers can be enhanced resulting from the electron-pulling effect when introducing TPT. Moreover, enhanced specific surface areas and positive skeleton charge due to the introduction of TPT may improve active sites exposure and Cr(VI) adsorption, thereby enhancing photocatalytic Cr(VI) reduction activity without the presence of any assisted scavengers. In addition, the photocatalytic mechanism (i.e. active species) were also studied and presented. This work confirmed an effective structure-performance regulation strategy on Fe-MOFs for photocatalytic Cr(VI) reduction.

20.
Front Endocrinol (Lausanne) ; 13: 893238, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36147561

RESUMO

Hypothalamus-pituitary-adrenal (HPA) axis plays critical roles in stress responses under challenging conditions such as hypoxia, via regulating gene expression and integrating activities of hypothalamus-pituitary-targets cells. However, the transcriptional regulatory mechanisms and signaling pathways of hypoxic stress in the pituitary remain to be defined. Here, we report that hypoxia induced dynamic changes in the transcription factors, hormones, and their receptors in the adult rat pituitary. Hypoxia-inducible factors (HIFs), oxidative phosphorylation, and cAMP signaling pathways were all differentially enriched in genes induced by hypoxic stress. In the pituitary gene network, hypoxia activated c-Fos and HIFs with specific pituitary transcription factors (Prop1), targeting the promoters of hormones and their receptors. HIF and its related signaling pathways can be a promising biomarker during acute or constant hypoxia. Hypoxia stimulated the transcription of marker genes for microglia, chemokines, and cytokine receptors of the inflammatory response. Corticotropin-releasing hormone receptor 1 (CRHR1) mediated the transcription of Pomc, Sstr2, and Hif2a, and regulated the function of HPA axis. Together with HIF, c-Fos initiated and modulated dynamic changes in the transcription of hormones and their receptors. The receptors were also implicated in the regulation of functions of target cells in the pituitary network under hypoxic stress. CRHR1 played an integrative role in the hypothalamus-pituitary-target axes. This study provides new evidence for CRHR1 involved changes of hormones, receptors, signaling molecules and pathways in the pituitary induced by hypoxia.

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