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1.
Sci Rep ; 10(1): 3689, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32111871

RESUMO

The root of Paeonia lactiflora Pall. (Chishao, CS) and Ligusticum chuanxiong Hort. (Chuanxiong, CX) were widely used as a drug pair in Chinese Medicine, and the combination of CS and CX showed a more significant inhibition on neuronal apoptosis in our previous study. In the present study, total paeony glycosides (TPGs) from CS and total ligustici phenolic acids (TLPAs) from CX were combined to evaluate the synergistic effects against focal cerebral ischemia both in vitro and in vivo. The combination of TPGs and TLPAs at 7:3 had the best anti-oxidative stress and anti-inflammatory effect on OGD-induced HUVEC. Additionally, the infarction area proportion and neuron apoptosis of rats by TPGs:TLPAs (7:3) was significantly lower than their alone in MCAO rats. Moreover, TPGs: TLPAs of 7:3 showed a more significant effect on decreasing the expression of MMP-2 and MMP-9, and increasing the protein expression or mRNA level of TIMP-1 than other combinations. The optimal ratio of TPGs and TLPAs at 7:3 could bring more remarkable protective effects against focal cerebral ischemia in MCAO rats by alleviating oxidative stress, inflammatory and neuronal apoptosis to protect the blood-brain barrier. Overall, the present study provided benefical evidence for clinical application of CS and CX as a "drug pair".

2.
Eur J Med Chem ; 192: 112197, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32172082

RESUMO

Protein misfolding diseases (PMDs) are chronic and progressive, with no effective therapy so far. Aggregation and misfolding of amyloidogenic proteins are closely associated with the onset and progression of PMDs, such as amyloid-ß (Aß) in Alzheimer's disease, α-Synuclein (α-Syn) in Parkinson's disease and human islet amyloid polypeptide (hIAPP) in type 2 diabetes. Inhibiting toxic aggregation of amyloidogenic proteins is regarded as a promising therapeutic approach in PMDs. The past decade has witnessed the rapid progresses of this field, dozens of inhibitors have been screened and verified in vitro and in vivo, demonstrating inhibitory effects against the aggregation and misfolding of amyloidogenic proteins, together with beneficial effects. Natural products are major sources of small molecule amyloid inhibitors, a number of natural derived compounds have been identified with great bioactivities and translational prospects. Here, we review the non-polyphenolic natural inhibitors that potentially applicable for PMDs treatment, along with their working mechanisms. Future directions are proposed for the development and clinical applications of these inhibitors.

3.
Nano Lett ; 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32119561

RESUMO

Simultaneous analysis based on encoded fluorophores suffers from potential crosstalk between fluorophores and the limited number of colors that can be practically resolved. Inspired by nontrivial temporal patterns in living organisms, we developed a DNA-templated probe by utilizing DNA polymerase (DNAP) for multiplexed detection of nucleic acids. These probes use differential delay times of signaling by a DNAP-mediated extension to distinguish different targets, which serve as the primers. Taking advantage of the high processivity and the controllable kinetics of DNAP, we find that multiplexed detection can be achieved in homogeneous solution using a single fluorophore. As a proof of concept, we developed assays for genomic DNA from four different bacteria. In addition, we designed and implemented probes to undergo a single oscillation in signal as an alternative way for multiplexing. We anticipate this approach will find broad applications not only in sensing but also in synthetic DNA nanosystems.

4.
EBioMedicine ; 53: 102689, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32114396

RESUMO

BACKGROUND: How the oncoprotein epidermal growth factor receptor (EGFR) evades proteolytic degradation and accumulates in non-small cell lung cancer (NSCLC) remains unclear, and ubiquitin pathway genes (UPGs) that are critical to NSCLC needs to be systematically identified. METHODS: A total of 696 UPGs (including E1, E2, E3, and deubiquitinases) were silenced by small interfering RNA (siRNA) library in NSCLC cells, the candidates were verified, and their significance was evaluated in patients with NSCLC. The effects of a candidate gene on EGFR were investigated in vitro and in vivo. FINDINGS: We report 31 candidates that are required for cell proliferation, with the E2 ubiquitin conjugase CDC34 as the most significant one. CDC34 is elevated in tumor tissues in 76 of 114 (66.7%) NSCLCs and inversely associated with prognosis, is higher in smoker patients than nonsmoker patients, and is induced by tobacco carcinogens in normal human lung epithelial cells. Forced expression of CDC34 promotes, whereas knockdown of CDC34 inhibits, NSCLC cell proliferation in vitro and in vivo. CDC34 competes with c-Cbl to bind Y1045 to inhibit polyubiquitination and degradation of EGFR. In EGFR-L858R and EGFR-T790M/Del (exon 19)-driven lung tumor growth in mouse models, knockdown of CDC34 significantly inhibits tumor formation. INTERPRETATION: These results demonstrate that an E2 enzyme is capable of competing with E3 ligase to stabilize substrates, and CDC34 represents an attractive therapeutic target for NSCLCs. FUNDING: National Key Research and Development Program of China, National Natural Science Foundation of China, and the CAMS Innovation Fund for Medical Sciences.

5.
Biomed Pharmacother ; 126: 110056, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32145589

RESUMO

Activation of G protein-coupled receptor 120 (GPR120) could inhibit apoptosis and inflammation in cerebral ischemic injury and liver ischemia-reperfusion injury. However, whether GPR120 agonism exerted potential for cisplatin-induced acute kidney injury and the involved mechanisms remained unknown. In our study, pharmacological activation of GPR120 by TUG891 treatment remarkably reduced the elevated serum creatinine level and attenuated tubular injury. Cisplatin triggered ATF6, PERK and IRE1 pathways of unfolded protein response (UPR) of ER stress in the injured kidney tissue, as well as the downstream molecules eIF2α, ATF4 and XBP1. Protein of ER stress-mediated apoptosis, CHOP, was overexpressed in the cisplatin group. Oral application of TUG891 displayed effective inhibition of ER stress and apoptosis. TUG891 treatment significantly decreased the TUNEL positive cells and the flow cytometry of HK-2 cells delineated the similar results that the apoptosis rates were considerably reduced in the TUG891 group compared to cisplatin group. Collectively, activation of GPR120 by TUG891 exhibited renal protection against cisplatin-induced AKI via suppressing ER-associated apoptosis in tubular epithelial cells.

6.
Chemosphere ; 252: 126483, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32197180

RESUMO

This study investigated the removal of Orange II by an electro-Fenton process using a novel recirculation flow-through reactor. The hydrogen peroxide was generated in-situ on the activated carbon fiber (ACF) modified with carbon black and polytetrafluoroethylene (PTFE). The modified ACF cathode was characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption study. In light of the production of H2O2 and removal of Orange II, the optimum weight percentage of PTFE in the mixture of carbon black and PTFE was 75%. The effects of some important operating parameters such as current and flow rate were investigated. The best Orange II removal reached 96.7% with mineralization efficiency of 55.4% at 120 min under the current of 100 mA, initial pH 3, Fe2+ 0.3 mM and the flow rate of 7 mL min-1. The cathode exhibited good regeneration ability and stability. OH was proved to be the main oxidizing species in this flow-through electro-Fenton system. This work demonstrated that such electro-Fenton process using modified ACF cathode was promising for the degradation of organic pollutants.

7.
Cell Death Dis ; 11(3): 183, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32170066

RESUMO

Exposure to nanomaterials (NMs) is an emerging threat to human health, and the understanding of their intracellular behavior and related toxic effects is urgently needed. Ferroptosis is a newly discovered, iron-mediated cell death that is distinctive from apoptosis or other cell-death pathways. No evidence currently exists for the effect of "iron free" engineered NMs on ferroptosis. We showed by several approaches that (1) zinc oxide nanoparticles (ZnO NPs)-induced cell death involves ferroptosis; (2) ZnO NPs-triggered ferroptosis is associated with elevation of reactive oxygen species (ROS) and lipid peroxidation, along with depletion of glutathione (GSH) and downregulation of glutathione peroxidase 4 (GPx4); (3) ZnO NPs disrupt intracellular iron homeostasis by orchestrating iron uptake, storage and export; (4) p53 largely participates in ZnO NPs-induced ferroptosis; and (5) ZnO particle remnants and dissolved zinc ion both contribute to ferroptosis. In conclusion, our data provide a new mechanistic rationale for ferroptosis as a novel cell-death phenotype induced by engineered NMs.

8.
J Nat Prod ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32191465

RESUMO

Overexpression of phosphopantetheinyl transferase (PPtase)-encoding genes sfp and svp in the marine-derived Verrucosispora sp. SCSIO 40062 led to the production of two new aminofuran monomers, proximicin F (1) and proximicin G (3) and a new dimer diproximicin A (2), along with two known compounds, proximicins B (4) and C (5). Their structures were unambiguously elucidated on the basis of detailed NMR spectroscopic analysis and high-resolution electrospray ionization mass spectrometry (HRESIMS) data. Proximicin B (4) showed moderate antibacterial activities against Staphylococcus aureus, methicillin-resistant S. aureus, and Bacillus subtilis.

9.
mBio ; 11(2)2020 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-32127451

RESUMO

Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multicopy gene family, termed the major surface glycoprotein (Msg) gene superfamily. This superfamily has been identified in all Pneumocystis species characterized to date, highlighting its important role in Pneumocystis biology. In this report, through a comprehensive and in-depth characterization of 459 msg genes from 7 Pneumocystis species, we demonstrate, for the first time, the phylogeny and evolution of conserved domains in Msg proteins and provide a detailed description of the classification, unique characteristics, and phylogenetic relatedness of five Msg families. We further describe, for the first time, the relative expression levels of individual msg families in two rodent Pneumocystis species, the substantial variability of the msg repertoires in P. carinii from laboratory and wild rats, and the distinct features of the expression site for the classic msg genes in Pneumocystis from 8 mammalian host species. Our analysis suggests multiple functions for this superfamily rather than just conferring antigenic variation to allow immune evasion as previously believed. This study provides a rich source of information that lays the foundation for the continued experimental exploration of the functions of the Msg superfamily in Pneumocystis biology.IMPORTANCE Pneumocystis continues to be a major cause of disease in humans with immunodeficiency, especially those with HIV/AIDS and organ transplants, and is being seen with increasing frequency worldwide in patients treated with immunodepleting monoclonal antibodies. Annual health care associated with Pneumocystis pneumonia costs ∼$475 million dollars in the United States alone. In addition to causing overt disease in immunodeficient individuals, Pneumocystis can cause subclinical infection or colonization in healthy individuals, which may play an important role in species preservation and disease transmission. Our work sheds new light on the diversity and complexity of the msg superfamily and strongly suggests that the versatility of this superfamily reflects multiple functions, including antigenic variation to allow immune evasion and optimal adaptation to host environmental conditions to promote efficient infection and transmission. These findings are essential to consider in developing new diagnostic and therapeutic strategies.

10.
mBio ; 11(2)2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32156824

RESUMO

Environmental exposure has a significant impact on human health. While some airborne fungi can cause life-threatening infections, the impact of environment on fungal spore dispersal and transmission is poorly understood. The democratization of shotgun metagenomics allows us to explore important questions about fungal propagation. We focus on Pneumocystis, a genus of host-specific fungi that infect mammals via airborne particles. In humans, Pneumocystis jirovecii causes lethal infections in immunocompromised patients if untreated, although its environmental reservoir and transmission route remain unclear. Here, we attempt to clarify, by analyzing human exposome metagenomic data sets, whether humans are exposed to different Pneumocystis species present in the air but only P. jirovecii cells are able to replicate or whether they are selectively exposed to P. jirovecii Our analysis supports the latter hypothesis, which is consistent with a local transmission model. These data also suggest that healthy carriers are a major driver for the transmission.

11.
Anal Chim Acta ; 1107: 40-47, 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32200900

RESUMO

As an ideal biomarker candidate, circulating tumor DNA (ctDNA) plays a vital role in noninvasive diagnosis of cancer. However, most traditional approaches for quantifying ctDNA are cumbersome and expensive. In the present work, a novel electrochemical biosensor based on nest hybridization chain reaction was proposed for the sensitive and specific detection of PIK3CA E545K ctDNA with a simple process. The nest hybridization chain reaction was initiated by the hybridization of two dumbbell-shaped DNA units which were assembled by two classes of well-designed DNA probes respectively, leading to the formation of a complex DNA structure. In the presence of target ctDNA, the amplified hybridization chain reaction products were captured by target ctDNA, resulting in a significant increase of electrochemical signal. Under the optimal conditions, the developed biosensor exhibited good analytical performance for the detection of target ctDNA with the linear range from 5 pM to 0.5 nM and the detection limit of 3 pM. Furthermore, this assay was successfully applied to the detection of ctDNA in spiked-in samples, pleural effusion and serum samples of malignant tumor patients. This simple and cost-effective sensing system holds great potentials for ctDNA detection and cancer diagnosis.

12.
Psychiatry Res ; 287: 112913, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32203751

RESUMO

Computerized cognitive remediation therapy (CCRT) has been found to generally improve cognition among patients with schizophrenia, but its effect on functioning has not been extensively studied. This study addressed this gap in the literature by investigating the effect of CCRT and its long-term efficacy among community-dwelling patients with schizophrenia. 157 Chinese patients with schizophrenia were recruited from communities and randomized to CCRT (n = 78) or treatment as usual (TAU; n = 79) groups for 12 weeks with 4-5 sessions per week. Neurocognition, functioning, and symptoms of participants were assessed at baseline, after treatment, and at the 6 month follow-up. The CCRT group showed significantly greater improvements than the TAU group regarding the MATRICS Consensus Cognitive Battery (MCCB) total score and social cognition score. Significant cognitive benefits for functioning were observed (Personal and Social Performance scale, PSP). Moreover, improvement of the MCCB total score mediated a positive effect on functional capacity (UCSD Performance-based Skills Assessment, UPSA), and mediated decreases in negative symptoms across both groups. CCRT improved social functioning and general cognitive functioning among community-dwelling patients with schizophrenia. These improvements persisted for 6 months after treatment. CCRT also led to improvements in functioning and symptom severity by modulating cognitive functioning.

13.
Bioinformatics ; 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32159762

RESUMO

MOTIVATION: Single-cell sequencing (SCS) data provide unprecedented insights into intratumoral heterogeneity. With SCS, we can better characterize clonal genotypes and reconstruct phylogenetic relationships of tumor cells/clones. However, SCS data are often error-prone, making their computational analysis challenging. RESULTS: To infer the clonal evolution in tumor from the error-prone SCS data, we developed an efficient computational framework, termed RobustClone. It recovers the true genotypes of subclones based on the low-rank matrix decomposition method with extended robust principal component analysis (RPCA), and reconstructs the subclonal evolutionary tree. RobustClone is a model-free method, which can be applied to both scSNV and scCNV data. It is efficient and scalable to large-scale datasets. We conducted a set of systematic evaluations on simulated datasets and demonstrated that RobustClone outperforms state-of-the-art methods in large-scale data both in accuracy and efficiency. We further validated RobustClone on 2 single-cell SNV and 2 single-cell CNV datasets and demonstrated that RobustClone could recover genotype matrix and infer the subclonal evolution tree accurately under various scenarios. In particular, RobustClone revealed the spatial progression patterns of subclonal evolution on the large-scale 10X Genomics scCNV breast cancer dataset. AVAILABILITY: RobustClone software is available at https://github.com/ucasdp/RobustClone. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

14.
Chembiochem ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32189429

RESUMO

A novel immobilization of penicillin G acylase (PGA) with a lanthanum-incorporated mesostructured cellular foam (La-MCF) support has been developed utilizing the interaction between the strong Lewis acid sites on the surface of La-MCF and the free amino groups of lysine residues of PGA. The La-MCF support was successfully synthesized by the in situ synthesis method through the addition of a citric acid (CA) complexant. The results of pyridine-IR show the presence of strong Lewis acid sites on the surface of the prepared La-MCF (with CA), attributed to the incorporation of lanthanum species into the framework of MCF. Based on the interaction with the strong Lewis acid sites, the enzymes can be firmly immobilized on the surface of the support. The results indicate that the PGA/La-MCF (with CA) exibits a high specific activity and greatly enhancing operational stability. For the hydrolysis of penicillin G potassium salt, the initial specific activity of PGA/La-MCF (with CA) reaches 10023 U/g. After recycled for 10 times, PGA/La-MCF (with CA) retains 89% of its initial specific activity, much higher than 77% of PGA/Si-MCF.

15.
Cell Death Dis ; 11(2): 103, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32029706

RESUMO

N6 methyladenosine (m6A) is one of the most prevalent epitranscriptomic modifications of mRNAs, and plays a critical role in various bioprocesses. Bone-derived mesenchymal stem cells (BMSCs) can attenuate apoptosis of nucleus pulposus cells (NPCs) under compression; however, the underlying mechanisms are poorly understood. This study showed that the level of m6A mRNA modifications was decreased, and the autophagic flux was increased in NPCs under compression when they were cocultured with BMSCs. We report that under coculture conditions, RNA demethylase ALKBH5-mediated FIP200 mRNA demethylation enhanced autophagic flux and attenuated the apoptosis of NPCs under compression. Specific silencing of ALKBH5 results in impaired autophagic flux and a higher proportion of apoptotic NPCs under compression, even when cocultured with BMSCs. Mechanistically, we further identify that the m6A "reader" YTHDF2 is likely to be involved in the regulation of autophagy, and lower m6A levels in the coding region of FIP200 lead to a reduction in YTHDF2-mediated mRNA degradation of FIP200, a core molecular component of the ULK1 complex that participates in the initiating process of autophagy. Taken together, our study reveals the roles of ALKBH5-mediated FIP200 mRNA demethylation in enhancing autophagy and reducing apoptosis in NPCs when cocultured with BMSCs.

16.
Mol Psychiatry ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32047265

RESUMO

Genome-wide association studies (GWAS) have successfully identified 145 loci implicated in schizophrenia (SCZ). However, the underlying mechanisms remain largely unknown. Here, we analyze 1497 RNA-seq data in combination with their genotype data and identify SNPs that are associated with expression throughout the genome by dissecting expression features to genes (eGene) and exon-exon junctions (eJunction). Then, we colocalize eGene and eJunction with SCZ GWAS using SMR and fine mapping. Multiple ChIP-seq data and DNA methylation data generated from brain were used for identifying the causal variants. Finally, we used a hypothesis-free (no SCZ risk loci considered) enrichment analysis to determine implicated pathways. We identified 171 genes and eight splicing junctions located within four genes (SNX19, ARL6IP4, APOPT1, and CYP2D6) that potentially contribute to SCZ susceptibility. Among the genes, CYP2D6 is significantly associated with SCZ SNPs in eGene and eJunction. In-depth examination of the CYP2D6 region revealed that a nonsynonymous single nucleotide variant rs16947 is strongly associated with a higher abundance of CYP2D6 exon 3 skipping junctions. While we found rs133377 and other functional SNPs in high linkage disequilibrium with rs16947 (r2 = 0.9539), histone acetylation analysis showed they are located within active transcription start sites. Furthermore, our data-driven enrichment analysis showed that CYP2D6 is significantly involved in drug metabolism of codeine, tamoxifen, and citalopram. Our study facilitates an understanding of the genetic architecture of SCZ and provides new drug targets.

17.
Bioengineered ; 11(1): 219-228, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32083506

RESUMO

Neuroblastoma (NB) is responsible for 15% of all childhood cancer deaths. Despite advances in treatment and disease management, the overall 5-year survival rates remain poor in high-risk disease (25-40%). It is well known that miR-145 functions as a tumor suppressor in several types of cancer. However, the impact of miR-145 on NB is still ambiguous. Our aim was to investigate the potential tumor suppressive role and mechanisms of miR-145 in high-risk neuroblastoma. Expression levels of miR-145 in tissues and cells were determined using RT-qPCR. The effect of miR-145 on cell viability was evaluated using MTT assays, apoptosis levels were determined using TUNEL staining, and the MTDH protein expression was determined using western blot and RT-PCR. Luciferase reporter plasmids were constructed to confirm direct targeting for MTDH. The results showed that miR-145 expression was significantly lower in high-risk MYCN amplified (MNA) tumors and low miR-145 expression was associated with worse EFS and OS in our cohort. Over-expression of miR-145 reduced cell viability and increased apoptosis in SH-SY-5Y cells. We identified MTDH as a direct target for miR-145 in SH-SY-5Y cells. Targeting MTDH has the similar results as miR-145 overexpression. Our findings suggest that low miR-145 expression was associated with poor prognosis in patients with NB, and the overexpression of miR-145 inhibited NB cells growth by down-regulating MTDH, thus providing a potential target for the development of microRNA-based approach for NB therapy.

18.
Sci Total Environ ; 717: 137090, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32065899

RESUMO

Thallium (Tl) pollution has attracted environmental attention due to its high toxicity, thus the cleanup of Tl from the environment is of significance. Titanate nanomaterials (TNMs) with different morphologies can be synthesized via a hydrothermal reaction under different conditions but the knowledge of the Tl(I) removal by them is limited. Our results indicated that TNM prepared at 130 °C exhibited a nanotubular appearance and a longer reaction time resulted in the formation of perfect nanotube, while that prepared at 180 °C exhibited a nanowire-like arrangement. The nanotubular and nanowire-like TNMs possessed approximately excellent Tl(I) adsorption capacities, wide pH, and temperature application ranges but different adsorption kinetics. Inorganic ions influenced the Tl(I) removal and the inhibiting effect of heavy metal ions followed the sequence Pb(II) > Cu(II) > Cd(II) > Zn(II). The anti-interference ability and selectivity of wire-like TNMs for Tl(I) removal were higher than those of tubular TNMs. High Tl(I) uptakes of tubular and wire-like TNMs were driven by the electrostatic attraction, ion exchange with Na+/H+, and complexation with -ONa functional groups in the interlayers and Ti-OH on the surfaces of TNMs as well as microprecipitation; while their adsorption configurations were different. TNMs are promising for potential applications in Tl(I) elimination from wastewater due to the high adsorption capacity and regenerability. This work indicates that TNMs synthesized under different conditions have the similar Tl(I) adsorption performances and the preparation of TNMs used for Tl(I) removal has an undemanding synthesis condition.

19.
Sci Rep ; 10(1): 2189, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32041971

RESUMO

The remediation of heavy metal-contaminated soils has attracted increased attention worldwide. The immobilization of metals to prevent their uptake by plants is an efficient way to remediate contaminated soils. This work aimed to seek the immobilization of cadmium in contaminated soils via a combination method. Flask experiments were performed to investigate the effects of hydroxyapatite (HAP) and the Cupriavidus sp. strain ZSK on soil pH and DTPA-extractable cadmium. Pot experiments were carried out to study the effects of the combined amendment on three plant species. The results showed that HAP has no obvious influence on the growth of the strain. With increasing concentrations of HAP, the soil pH increased, and the DTPA-extractable Cd decreased. Via the combined amendment of the strain and HAP (SH), the DTPA-extractable Cd in the soil decreased by 58.2%. With the combined amendment of the SH, the cadmium accumulation in ramie, dandelion, and daisy decreased by 44.9%, 51.0%, and 38.7%, respectively. Moreover, the combined amendment somewhat benefitted the growth of the three plant species and significantly decreased the biosorption of cadmium. These results suggest that the immobilization by the SH combination is a potential method to decrease the available cadmium in the soil and the cadmium accumulation in plants.

20.
Sensors (Basel) ; 20(4)2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32085588

RESUMO

Timely calls for help can really make a difference for elders who suffer from falls, particularly in private locations. Considering privacy protection and convenience for the users, in this paper, we approach the problem by using impulse-radio ultra-wideband (IR-UWB) monostatic radar and propose a learning model that combines convolutional layers and convolutional long short term memory (ConvLSTM) to extract robust spatiotemporal features for fall detection. The performance of the proposed scheme was evaluated in terms of accuracy, sensitivity, and specificity. The results show that the proposed method outperforms convolutional neural network (CNN)-based methods. Of the six activities we investigated, the proposed method can achieve a sensitivity of 95% and a specificity of 92.6% at a range of 8 meters. Further tests in a heavily furnished lounge environment showed that the model can detect falls with more than 90% sensitivity, even without re-training effort. The proposed method can detect falls without exposing the identity of the users. Thus, the proposed method is ideal for room-level fall detection in privacy-prioritized scenarios.

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