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1.
J Nanosci Nanotechnol ; 20(4): 2205-2213, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492229

RESUMO

In this study, three-dimensional (3D) networked porous polyvinyl alcohol/sodium alginate/graphene oxide (PVA/SA/GO) spherical composites were fabricated by the sol-gel method and employed as adsorbents for the adsorption of methylene blue (MB) in aqueous solution. The obtained samples were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermal property analysis, and nitrogen adsorption/desorption isotherms. Moreover, the adsorption properties for MB were investigated by batch experiments. The pseudo-first-order and pseudo-second-order equations were used to fit the adsorption kinetics data, and the Langmuir and Freundlich isothermal models were used to analyze the adsorption isothermals. The results showed that the spherical composites had 3D porous structures, and GO, PVA and SA were fused and linked together by self-assembly, physical intertwining, hydrogen bonding, and Ca2+ and boric acid crosslinking. The maximum adsorption capacity of the 3D porous PVA/SA/GO spherical composites for MB was 759.3 mg/g. The adsorption kinetics had a better agreement with the pseudo-secondorder equation than the pseudo-first-order equation, and the equilibrium data followed the Freundlich model.

2.
J Immunol Res ; 2019: 9164202, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31482100

RESUMO

Collectin-12 (collectin placenta 1, CL-P1, or CL-12) is a newly identified pattern recognition molecule of the innate immune system. Recent evidences show that CL-12 plays important roles not only in innate immune protection against certain clinically important pathogens but also in scavenging of host molecules, leukocyte recruitment, and cancer metastasis. Furthermore, CL-12 has been shown to be associated with the pathogenesis of human diseases such as Alzheimer's disease and multiple sclerosis lesion development. Therefore, the functional consequence of CL-12 remains intriguing and awaits further elucidation. However, available protocols for the purification of recombinant CL-12 with high purity are laborious and inefficient and hamper further functional studies. Here, we report a simple, rapid, and efficient solution to obtain biologically active CL-12 with high purity. We established stable transfected Flp-In™-CHO cells expressing the recombinant CL-12 extracellular domain in high amounts. Recombinant CL-12 was purified from cell culture supernatants using a 3-step rapid purification procedure utilizing disposable affinity and ion exchange minicolumns. Purified recombinant CL-12 adopted an oligomeric structure with monomers, dimers, and trimers and retained its binding capacity towards the A. fumigatus strain that has been described before. Furthermore, we demonstrated the opsonic properties towards eight clinical isolates of A. fumigatus strains and diverse clinically important fungal pathogens. Purified recombinant CL-12 revealed a differential binding capacity towards selected fungal pathogens in vitro. In conclusion, we demonstrate a rapid and efficient purification solution for further biochemical and functional characterization of CL-12 and reveal opsonic properties of CL-12 towards diverse fungal pathogens.

3.
Microbiologyopen ; : e929, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31482690

RESUMO

The Siamese crocodile (Crocodylus siamensis) is a freshwater, endangered crocodile with high economic value in the farming industry. Gut microflora plays an essential role in host physiological activity, and it contributes significantly to both the health and diseased states of animals. However, thus far, no study has focused on the correlation between diseases and intestinal bacterial communities in crocodilians. Here, we first compared the composition and function of gut microbial communities in captive juvenile C. siamensis suffering from anorexia and healthy crocodile controls using deep amplicon sequencing. The gut microbial diversity of anorexic crocodiles was much lower than the healthy individuals. Obvious changes in gut microbial composition were observed between sick and healthy crocodiles, except for Cetobacterium somerae of phylum Fusobacteria. In particular, the abundance of Bacteroides luti, Clostridium disporicum, Plesiomonas shigelloides, and Odoribacter sp. in the gut flora of healthy crocodiles was distinctly higher than the diseased group. Conversely, the species Edwardsiella tarda was overrepresented in the gut of anorexic crocodiles compared to the healthy group. Furthermore, in anorexic crocodiles, the predicted microbial functions that were related to amino acid metabolism, biosynthesis of other secondary metabolites, nucleotide metabolism, replication and repair, and translation were significantly reduced, while signal transduction was significantly enriched. These findings of the present study provide a reference to enrich the field of gut microorganism studies in crocodilians and suggest that alterations in the composition and function of gut bacteria in C. siamensis juveniles may be associated with anorexia in crocodiles.

4.
Opt Express ; 27(15): 21741-21749, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31510245

RESUMO

The nonlinear optical properties of Bi2Se3 nanosheets of different diameters were studied using the femtosecond Z-scan technique. As the excitation wavelength increased from that for resonant absorption to non-resonant absorption, the nonlinear absorption of the smaller nanosheets with diameter of 30 or 80 nm changed from saturation to reverse saturation and the corresponding relaxation times decreased, unlike for larger nanosheets with diameter of 2000nm. The more sensitive nonlinear refractive index changed from positive to negative for all the nanosheets, when the excitation wavelengths were near-resonance absorption wavelengths. A simplified model similar to noble metal nanomaterials explains the observations suitably. The reversal in optical nonlinearities of Bi2Se3 nanosheets vastly enhances their properties.

5.
Medicine (Baltimore) ; 98(37): e17114, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517847

RESUMO

BACKGROUND: We will investigate the efficacy and safety of weekly cisplatin (WC) for treatment of patients with breast cancer (BC) systematically. METHODS: This study will describe and critically appraise shared decision approaches used in randomized controlled trials of WC for treatment of patients with BC. We will comprehensively search the following databases: PubMed, EMBASE, Web of Science, Cochrane Library, CINAHL, PsycINFO, Allied and Complementary Medicine Database, Wanfang, and Chinese Biomedical Literature Database from inception through July 1, 2019. We will utilize RevMan V.5.3 software (London, UK) for statistical analysis. RESULTS: This study will systematically explore the efficacy and safety of WC for the treatment of patients with BC through evaluating primary outcomes of overall survival, pathological complete response; and secondary outcomes of cancer-specific survival, recurrence-free survival, disease-free survival, quality of life, and toxicities. CONCLUSION: This study will provide latest evidence of WC for the treatment of patients with BC. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42019145358.

6.
Am J Trop Med Hyg ; 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31436160

RESUMO

A 50-year-old Chinese woman with a history of weakness and paroxysmal seizures of the left limb presented to our hospital with a ten-day history of neck pain. Imaging showed that there was a mass in the frontal lobe of her brain. On resection of the mass, a motile worm was identified. Morphologic study and molecular analysis of the mitochondrial COX1 and 28S rRNA genes of the worm extracted from the brain identified the causative agent as Spirometra mansoni. Homology search of the PCR-amplified products from the case was conducted against gene fragments from local wild frogs. High homology was found between them, showing her likely exposure was frog consumption.

7.
J Biomol Struct Dyn ; : 1-10, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31402760

RESUMO

PTP-MEG2 plays a significant role in insulin production and is able to enhance insulin signaling and improve insulin sensitivity. So, PTP-MEG2 inhibitors are closely associated with type 2 diabetes therapy. A series of novel (R)-5-methylthiazolidin-4-one derivatives were designed and synthesized, and their PTP-MEG2 inhibitory activities (IC50) were determined. Among the desired compounds, 1h shares the highest inhibitory activity (IC50 = 1.34 µM) against PTP-MEG2. Additionally, various post-dynamic analyses confirmed that when compound 1h bound to the PTP-MEG2, the protein conformations became unstable and the function of the pTyr recognition loop (Asn331-Cys338) would be disturbed. And thus, the ideal conformations needed for the catalytic activity was difficult to be maintained. In brief, these might be how the compound 1h worked. Furthermore, we also found that the key residues Arg332 would play a critical role in disturbing the residue interactions. Abbreviations DCCM dynamic cross-correlation mapping DMF N,N-dimethylformamide DSSP definition of secondary structure of proteins FOXO forkhead transcription factors MD molecular dynamics PCA principal component analysis PDB protein data bank PTKs protein tyrosine kinases PTPs protein tyrosine phosphatases PTP-MEG2 megakaryocyte protein tyrosine phosphatase 2 RIN residue interaction network RING Residue Interaction Network Generator RMSD root means square deviation RMSF root mean square fluctuation Communicated by Ramaswamy H. Sarma.

8.
BMC Pregnancy Childbirth ; 19(1): 294, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31409379

RESUMO

BACKGROUND: The risk of venous thromboembolism (VTE) during pregnancy and puerperal periods is significantly higher than during the non-pregnant period and is one of the major causes of maternal mortality. Developed countries have promulgated guidelines for risk assessment and prevention of maternal VTE, and standardized management has led to a significant reduction in maternal mortality. However, there is a paucity of relevant research related to pregnancy and puerperal VTE in China. METHODS/DESIGN: We will perform a prospective cohort study and recruit 13,000 pregnant women from 2018 to 2020 in Beijing, China. VTE risk assessment will be conducted using the Royal College of Obstetricians and Gynaecologists (RCOG) pregnancy and puerperal VTE risk-assessment-scoring tool during early and late pregnancy, as well as during the puerperal period. Venous ultrasonography of lower extremities, routine blood tests, and coagulation parameters will be examined. These VTE risk assessments will be performed again if patients have VTE-related symptoms during their pregnancies, or if any of the following occur: (1) patients are hospitalized over 7 days due to any pregnancy complications; (2) patients are placed under strict bed rest for ≥ 3 days to prevent miscarriage. For patients with a confirmed diagnosis of VTE, treatment and follow-up plans will be decided jointly by the obstetricians, vascular surgeons, and pulmonologists. All patients in the study will be followed up by dedicated healthcare providers for up to 42 days postpartum. Statistical analyses will be performed to test the feasibility of the RCOG scoring tool for the Chinese population. The RCOG scoring tool will then be revised based upon the characteristics of the Chinese population, and the revised assessment scoring tool will then be tested in the cohort to evaluate its efficacy. Finally, a pregnancy and puerperal VTE risk-assessment tool will be proposed based on our study results. DISCUSSION: This study will establish a preliminary VTE risk-assessment tool that is applicable to pregnant and puerperal women in China and provide guidelines for further thrombophylactic interventions. Furthermore, we wish to draw increased attention to pregnancy-associated VTE to reduce VTE-related mortality. TRIAL REGISTRATION: Chi CTR1800015848 (04/24/2018).

9.
ACS Nano ; 13(8): 8903-8916, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31374171

RESUMO

A major concern about glucose oxidase (GOx)-mediated cancer starvation therapy is its ability to induce serious oxidative damage to normal tissues through the massive production of H2O2 byproducts in the oxygen-involved glucose decomposition reaction, which may be addressed by using a H2O2 scavenger, known as an antioxidation agent. Surprisingly, H2O2 removal accelerates the aerobic glycometabolism of tumors by activating the H2O2-dependent "redox signaling" pathway of cancer cells. Simultaneous oxygen depletion further aggravates tumor hypoxia to increase the toxicity of a bioreductive prodrug, such as tirapazamine (TPZ), thereby improving the effectiveness of cancer starvation therapy and bioreductive chemotherapy. Herein, a "nitrogen-protected silica template" method is proposed to design a nanoantioxidant called an organosilica-based hollow mesoporous bilirubin nanoparticle (HMBRN), which can act as an excellent nanocarrier to codeliver GOx and TPZ. In addition to efficient removal of H2O2 for self-protection of normal tissues via antioxidation, GOx/TPZ-coloaded HMBRN can also rapidly deplete intratumoral glucose/oxygen to promote a synergistic starvation-enhanced bioreductive chemotherapeutic effect for the substantial suppression of solid tumor growth. Distinct from the simple combination of two treatments, this study introduces antioxidation-activated self-protection nanotechnology for the significant improvement of tumor-specific deoxygenation-driven synergistic treatment efficacy without additional external energy input, thus realizing the renaissance of precise endogenous cancer therapy with negligible side effects.

10.
Eur Radiol ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31468159

RESUMO

OBJECTIVE: To investigate the discriminative capabilities of different machine learning-based classification models on the differentiation of small (< 4 cm) renal angiomyolipoma without visible fat (AMLwvf) and renal cell carcinoma (RCC). METHODS: This study retrospectively collected 163 patients with pathologically proven small renal mass, including 118 RCC and 45 AMLwvf patients. Target region of interest (ROI) delineation, followed by texture feature extraction, was performed on a representative slice with the largest lesion area on each phase of the four-phase CT images. Fifteen concatenations of the four-phasic features were fed into 224 classification models (built with 8 classifiers and 28 feature selection methods), classification performances of the 3360 resultant discriminative models were compared, and the top-ranked features were analyzed. RESULTS: Image features extracted from the unenhanced phase (UP) CT image demonstrated dominant classification performances over features from other three phases. The two discriminative models "SVM + t_score" and "SVM + relief" achieved the highest classification AUC of 0.90. The 10 top-ranked features from UP included 1 shape feature, 5 first-order statistics features, and 4 texture features, where the shape feature and the first-order statistics features showed superior discriminative capabilities in differentiating RCC vs. AMLwvf through the t-SNE visualization. CONCLUSION: Image features extracted from UP are sufficient to generate accurate differentiation between AMLwvf and RCC using machine learning-based classification model. KEY POINTS: • Radiomics extracted from unenhanced CT are sufficient to accurately differentiate angiomyolipoma without visible fat and renal cell carcinoma using machine learning-based classification model. • The highest discriminative models achieved an AUC of 0.90 and were based on the analysis of unenhanced CT, alone or in association with images obtained at the nephrographic phase. • Features related to shape and to histogram analysis (first-order statistics) showed superior discrimination compared with gray-level distribution of the image (second-order statistics, commonly called texture features).

11.
Artigo em Inglês | MEDLINE | ID: mdl-31443054

RESUMO

An increasing number of neural memory networks have been developed, leading to the need for a systematic approach to analyze and compare their underlying memory structures. Thus, in this paper, we first create a framework for memory organization and then compare four popular dynamic models: vanilla recurrent neural network, long short-term memory, neural stack, and neural RAM. This analysis helps to open the dynamic neural networks' black box from the memory usage prospective. Accordingly, a taxonomy for these networks and their variants is proposed and proved using a unifying architecture. With the taxonomy, both network architectures and learning tasks are classified into four classes, and a one-to-one mapping is built between them to help practitioners select the appropriate architecture. To exemplify each task type, four synthetic tasks with different memory requirements are selected. Moreover, we use some signal processing applications and two natural language processing applications to evaluate the methodology in a realistic setting.

12.
Cell Death Differ ; 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31406304

RESUMO

ß-transducin repeat-containing protein (ß-TrCP), one of the best-characterized substrate recognition components of the SKP1-CUL1-F-box (SCF) E3 ligase, has two distinct paralogs, ß-TrCP1 and ß-TrCP2, expressed in mammals. Through governing the ubiquitination and degradation of numerous key regulators, ß-TrCP1/2 regulates various cellular physiological and pathological processes. However, whether and how these two proteins cross talk and whether they regulate cell autophagy and proliferation in different manners is completely unknown. Herein, we report that ß-TrCP1 and ß-TrCP2 are the physiological substrates of SCF E3 ligase and target each other for degradation that is dependent on their ß-TrCP degron sequences. Furthermore, glucose deprivation activates AMPK kinase to phosphorylate ß-TrCP1 and promotes the subsequent ubiquitination and degradation of ß-TrCP1 by ß-TrCP2, but does not promote ß-TrCP2 degradation by ß-TrCP1. Finally, we found that ß-TrCP2, not ß-TrCP1, preferentially degrades DEPTOR and REDD1, the inhibitors of mTORC1, to activate mTORC1, leading to autophagy inhibition and cell growth. Thus, our study demonstrates that ß-TrCP1 and ß-TrCP2 mutually target each other for degradation and that ß-TrCP2 acts as a dominant paralog in the regulation of cell autophagy and growth, which might be a promising anticancer target.

13.
Biotechnol Adv ; : 107423, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31398397

RESUMO

Seed coating is a technique of covering seeds with adhesive agents to improve seed performance and plant establishment while reducing production cost. To meet the needs of development of precision agriculture, seed coating has been widely used in agriculture as an effective means to alleviate biotic and abiotic stresses, thus enhancing crop growth, yield, and health. Plant growth promoting microorganisms (PGPM) are recognized as essential contributors to improving agricultural productivity via direct application to the rhizosphere and plant tissues, or seed inoculation. However, during conventional inoculation processes, several factors such as insufficient microbial survival, hindrance in the application of biocontrol inocula to the seeds and exposure to unsuitable temperature and light in subsequent seed storage, force us to explore efficient and reliable microbial application tools. Recently, biological seed coating with PGPM is proposed as an alternative to conventional seed treatment (such as fertilizer and protection products) due to its ecological safety and socio-economic aspects. In this review, microbial seed coating technology and its contribution to sustainable precision agriculture are well discussed and highlighted in the extensive table and elaborate schematic drawings.

14.
Zhen Ci Yan Jiu ; 44(6): 439-42, 2019 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-31368268

RESUMO

OBJECTIVE: To compare the clinical efficacy of soft-tissue relaxing needling and electroacupuncture (EA) in the treatment of knee osteoarthritis (KOA), so as to explore a new and more effective therapy for KOA. METHODS: Forty patients with KOA who met our diagnostic criteria were randomly and equally divided into acupuncture group and soft-tissue relaxing needling (relaxing-needling) group. EA (20 Hz, a tolerable strength and duration of 20 min) was applied to the unilateral Neixiyan(EX-LE5) and Waixiyan(EX-LE5), and manual acupuncture stimulation was applied to Heding(EX-LE2), Xuehai (SP10), Xiyangguan (GB33), Liangqiu(ST34), Yanglingquan(GB34) and Yinlingquan(SP9) on the affected side by using uniform reinforcing-reducing technique. In the relaxing-needling group, after identifying the tender point and nodule-like or stiff-strip-muscle spot at the affected limb by palpation, we used filiform needles to insert into them, then, made a longitudinal separation or point-like pricking. The visual analog scale (VAS) pain score, knee flexion activity (range of motion, ROM), and the knee osteoarthritis severity (Lequesne index, composed of daily living, walking distance and pain) were measured before and after the treatment. The therapeutic effect was assessed by consulting the Guiding Principles for Researching New Drugs of Traditional Chinese Medicine (2002) and Criteria for Diagnosis and Assessment of Therapeutic Effect of Syndromes or Illnesses of Traditional Chinese Medicine (1994). RESULTS: After the treatment, the VAS score and Lequesne index were significantly decreased in both acupuncture and relaxing-needling groups (P<0.001), and the ROM score was considerably increased in both groups in comparison with their own pre-treatment (P<0.001). The difference values of VAS score and Lequesne index between pre- and post-treatment were significantly higher in the relaxing-needling group than in the acupuncture group (P<0.05). Of the two 20 cases in the relaxing-needling and acupuncture groups, 8 and 3 experienced a remarkable improvement in their symptoms, 10 and 13 were effective, 2 and 4 failed, with the effective rate being 90.0% and 80.0%, respectively. No significant difference was found between the two groups in the difference value of ROM score and the effective rate (P>0.05).. CONCLUSION: Both relaxing-needling and EA therapies are comparable in the therapeutic effect for KOA, and the former is superior to the latter in reducing the joint pain and improving the knee joint locomotor function, thus being worthy of clinical application.


Assuntos
Eletroacupuntura , Osteoartrite do Joelho , Pontos de Acupuntura , Humanos , Medicina Tradicional Chinesa , Osteoartrite do Joelho/terapia , Resultado do Tratamento
15.
Bioorg Med Chem ; 27(19): 115041, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31402203

RESUMO

The efficient radiosynthesis of biomolecules utilizing minute quantities of maleimide substrate is important for availability of novel peptide molecular imaging agents. We evaluated both 3-18F-fluoropropane-1-thiol and 2-(2-(2-(2-18F-fluoroethoxy)ethoxy)ethoxy)ethane-1-thiol (18F-fluoro-PEG4 thiol) as prosthetic groups for radiolabeling under physiological conditions. The precursor employed a benzoate for protection of the thiol and an arylsulfonate leaving group. The radiofluorination was fully automated on an Eckert & Ziegler synthesis system using standard Kryptofix222/K2CO3 conditions. In order to minimize the amount of biological molecule required for subsequent conjugation, the intermediates, S-(3-18F-fluoropropyl) benzothioate and 18F-fluoro-PEG4 benzothioate, were purified by HPLC. The intermediates were isolated from the HPLC in yields of 37-47% and 28-35%, respectively, and retrieved from eluate using solid phase extraction. Treatment of the benzothioates with sodium methoxide followed by acetic acid provided the free thiols. The desired maleimide substrate in acetonitrile or phosphate buffer was then added and incubated at room temperature for 15 min. The final radiolabeled bioconjugate was purified on a separate HPLC or NAP-5 column. Maleimides utilized for the coupling reaction included phenyl maleimide, an Evans Blue maleimide derivative, a dimeric RGDfK maleimide (E[c(RGDfK)]2), two aptamer maleimides, and PSMA maleimide derivative. Isolated radiochemical yields (non-decay corrected) of maleimide addition products based on starting 18F-fluoride ranged from 6 to 22% in a synthesis time of about 90 min. 18F-thiol prosthetic groups were further tested in vivo by conjugation to E[c(RGDfK)]2 maleimide in a U87MG xenograft model. PET studies demonstrated similar tumor accumulation of both prosthetic groups. 18F-fluoro-PEG4-S-E[c(RGDfK)]2 displayed a somewhat favorable pharmacokinetics compared to 18F-fluoropropyl-S-E[c(RGDfK)]2. Bone uptake was low for both indicating in vivo stability.

16.
Mol Med Rep ; 20(3): 2303-2315, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31322177

RESUMO

Restraint water­immersion stress (RWIS) can induce a gastric mucosal lesions within a few hours. The medial prefrontal cortex (mPFC) is involved in the RWIS process. The present study investigated the modulatory effects and molecular mechanisms of the mPFC on gastric function under an RWIS state. Male Wistar rats were divided into four groups; namely, the control, RWIS 4 h (RWIS for 4 h only), sham­operated and bilateral­lesioned (bilateral­lesioned mPFC) groups. The gastric erosion index (EI) and gastric motility (GM) were determined, and the proteomic profiles of the mPFC were assessed by isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two­dimensional liquid chromatography and tandem mass spectrometry. Additionally, iTRAQ results were verified by western blot analysis. Compared with the RWIS 4 h group and the sham­control group, the bilateral­lesioned group exhibited a significantly lower EI (P<0.01). In the bilateral­lesioned group, RWIS led to a significant decrease in EI and GM. When comparing the control and RWIS 4 h groups, 129 dysregulated proteins were identified, of which 88 were upregulated and 41 were downregulated. Gene Ontology functional analysis demonstrated that 29 dysregulated proteins, including postsynaptic density protein 95, were directly associated with axon morphology, axon growth and synaptic plasticity. Ingenuity pathway analysis revealed that the dysregulated proteins were mainly involved in neurological disease signaling pathways, including the NF­κB and ERK signaling pathways. These data indicated that the presence of the mPFC exacerbates gastric mucosal injury in awake rats during RWIS. Although the quantitative proteomic analysis elucidated the nervous system molecular targets associated with the production of gastric mucosal lesions, such as the role of PSD95. The underlying molecular mechanisms of synaptic plasticity need to be further elucidated.

17.
Transbound Emerg Dis ; 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-31342637

RESUMO

Porcine circovirus type 2 (PCV2) has been causing huge economic losses in Chinese swine herds since it was first identified in China in 1999. Genotypes of PCV2 except for PCV2c coexist in swine herds in China, which may facilitate virus recombination. In the current study, six novel PCV2 strains were detected in China, and these strains shared high nucleotide similarity of the Rep gene with the PCV2c strain DK1987PMWSfree and high homology of the Cap gene with PCV2d. Genome sequence analysis revealed that the complete genomes of these strains were 1767 nucleotides (nt) in length and shared 99.8%-99.9% nucleotide identity with each other and 91.7%-98.7% with representative strains. Phylogenetic analysis, sequencing analysis, base-by-base comparisons and comprehensive recombination analysis demonstrated that these six strains originated from recombination within the Rep gene between PCV2c and PCV2d strains. Surprisingly, further investigation through theoretical recombination analysis of Chinese PCV2 GenBank sequences showed that these novel patterns of recombinant PCV2 strains have been generated since 2010. Collectively, our findings provide additional evidence of inter-genotypic recombination of PCV2.

18.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(6): 563-568, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31292062

RESUMO

Melatonin (MLT) is an amine hormone secreted mainly by the pineal gland. The main physiological function of MLT is to regulate the circadian rhythm. Recent studies have shown that MLT could affect the immune system through several ways to regulate the function of immune system. In infectious diseases, MLT can regulate the number of immune cells and the expression of cytokines. Moreover, MLT may enhance the function of the immune system by reducing the secretion of inflammatory factors, down-regulating the adhesion of leukocytes and anti-oxidation, which may reduce the pathology caused by inflammation damage. Meanwhile, MLT could play a role against pathogens infection by directly inhibiting the replication and proliferation of pathogens. Therefore, MLT may be considered as a novel target and a new method for the clinical treatment of infectious diseases. This article reviews the progress of the effects and mechanisms of MLT in regulating the immune system and the important role in infectious diseases.


Assuntos
Doenças Transmissíveis/fisiopatologia , Sistema Imunitário/fisiologia , Melatonina/fisiologia , Citocinas/imunologia , Humanos , Inflamação/imunologia
19.
Anticancer Drugs ; 30(9): 948-952, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31283544

RESUMO

Pegylated liposomal doxorubicin (Peg-Dox) treatment resulted in a good outcome for patients with lymphoma and multiple myeloma, with reduced cardiotoxicity and an improved pharmacokinetic profile when compared to those of conventional doxorubicin. However, the use of Peg-Dox in myeloid neoplasms remains poorly studied. In this study, we first tested the role of Peg-Dox in the killing of myeloid cell lines and of primary myeloid leukemia cells. Then, a Peg-Dox-based protocol was used to treat patients with myeloid neoplasms. The results showed that the Peg-Dox and Peg-Dox-based protocols had a similar killing ability in myeloid cell lines and in primary myeloid leukemia cells compared to that of conventional doxorubicin. The complete remission rate was 87.5% and 100% for patients with refractory/relapsed acute myeloid leukemia and myelodysplastic syndrome with excess blasts, respectively, after treatment with Peg-Dox. All patients developed grade 3 or 4 hematological toxicity and recovered approximately 2 weeks after completing chemotherapy. No deaths or other severe complications were reported. Our results showed that Peg-Dox can be used in the treatment of myeloid neoplasms with high rates of complete remission and with mild complications.

20.
Mol Med Rep ; 20(2): 1781-1789, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31257544

RESUMO

GC binding factor 2 (GCF2) is a transcriptional repressor that inhibits the transcription of GC­rich promoters, thereby regulating biological processes, including proliferation. However, the role of GCF2 in vascular smooth muscle cells (VSMCs) remains unclear. The level of α­smooth muscle (α­SM) actin was determined by immunofluorescence. Cell viability, migration and invasion were analyzed using Cell Counting Kit­8, wound healing and Transwell assays, respectively. Apoptosis and cell cycle progression were determined using flow cytometry. The expressions of Bcl­2, Bax, cleaved caspase­3, cyclin E, CDK2 and the CDK inhibitor p21 were determined by reverse transcription­quantitative (RT­q)PCR and western blot analysis. RT­qPCR was performed to analyze the levels of GCF2 and western blot analysis was conducted to determine the phosphorylation levels of PI3K and AKT. α­SM actin was found to be expressed in VSMCs. Cell viability, migration and invasion were inhibited by small interfering (si)RNA targeting GCF2. Changes in the expression levels of Bcl­2, Bax and cleaved caspase­3 showed that the pro­apoptotic capacity of the cells was increased by siGCF2. Cell cycle arrest in the G0/G1 phase was induced by siGCF2, which was accompanied by changes in the levels of cyclin E, CDK2 and p21. Furthermore, phosphorylation of PI3K and AKT was suppressed by siGCF2. However, the inhibitory effects of siGCF2 on cell viability, migration and invasion were increased by insulin­like growth factor 1, which is a specific agonist of AKT. The anti­proliferative activity of siGCF2 may be associated with the PI3K/AKT pathway in VSMCs.

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