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1.
Artigo em Inglês | MEDLINE | ID: mdl-32069397

RESUMO

Many recent efforts on electrode design for advanced Li-ion batteries (LIBs) are often devoted to increasing the gravimetric capacity, but little attention is paid to the volumetric capacity which is more critical for practical application. Though the alloying-type anode materials are quite attractive, challenge is that they must be composited with large amount of carbon materials (e.g. GO, rGO, CNT) to buffer their large volume change, which would undoubtedly sacrifice the volumetric energy density of the whole electrode due to the carbon's low tap density (0.05 g/cm3). Herein, we propose the unique layered Sb2Te3, which possesses high conductivity and large volumetric capacity (3419 mAh/cm3), to be served as the alternative anode for LIBs. Furthermore, we introduce natural graphite, which is low price and with high density (2.25 g/cm3), into Sb2Te3 to successfully build a novel Sb2Te3@Gra composite in which the Sb2Te3 particles are wrapped by graphite layers. Interestingly, this modified Sb2Te3@Gra exhibits much more superior cycle stability (570 mAh/g after 200 cycles, 96% retention) than pure Sb2Te3 (130 mAh/g after 200 cycles, 22% retention), while keeping its original large volumetric capacity output (3200 mAh/cm3) at the same time. More specially, it enables a reversible structure recovery of Sb2Te3, guaranteeing the electrode integrity and cyclability. These extraordinary phenomena are investigated in detail, whose results display that the outer graphite layer plays an important role by facilitating the intimate contact with Sb2Te3 particles and protecting them from pulverization. Besides, such graphite layer greatly promotes the electron-transfer during lithiation, helping to improve the rate capability (372 mAh/g at 2000 mA/g, 60% retention). Consequently, the assembled Sb2Te3//LiCoO2 full cell delivers a large capacity of 500 mAh/g, with stable discharge plateau and cycle stability, revealing its high potential for practical application.

3.
Brief Bioinform ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32010933

RESUMO

Gastric cancer (GC) continues to be one of the major causes of cancer deaths worldwide. Meanwhile, liquid biopsies have received extensive attention in the screening and detection of cancer along with better understanding and clinical practice of biomarkers. In this work, 58 routine blood biochemical indices were tentatively used as integrated markers, which further expanded the scope of liquid biopsies and a discrimination system for GC consisting of 17 top-ranked indices, elaborated by random forest method was constructed to assist in preliminary assessment prior to histological and gastroscopic diagnosis based on the test data of a total of 2951 samples. The selected indices are composed of eight routine blood indices (MO%, IG#, IG%, EO%, P-LCR, RDW-SD, HCT and RDW-CV) and nine blood biochemical indices (TP, AMY, GLO, CK, CHO, CK-MB, TG, ALB and γ-GGT). The system presented a robust classification performance, which can quickly distinguish GC from other stomach diseases, different cancers and healthy people with sensitivity, specificity, total accuracy and area under the curve of 0.9067, 0.9216, 0.9138 and 0.9720 for the cross-validation set, respectively. Besides, this system can not only provide an innovative strategy to facilitate rapid and real-time GC identification, but also reveal the remote correlation between GC and these routine blood biochemical parameters, which helped to unravel the hidden association of these parameters with GC and serve as the basis for subsequent studies of the clinical value in prevention program and surveillance management for GC. The identification system, called GC discrimination, is now available online at http://lishuyan.lzu.edu.cn/GC/.

4.
J Biomol Struct Dyn ; : 1-29, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32036779

RESUMO

SHP2 is a non-receptor protein tyrosine phosphatase (PTP) encoded by the PTPN11 gene involved in cell death pathway (PD-1/PD-L1) and cell growth and differentiation pathway (MAPK). Moreover, mutations in SHP2 have been implicated in Leopard syndrome(LS),Noonan syndrome (NS), juvenile myelomonocytic leukemia (JMML) and several types of cancer and solid tumors. Thus, SHP2 inhibitors are much needed reagents for evaluation of SHP2 as a therapeutic target. A series of novel ethyl 4-(phenoxymethyl)-2-phenylthiazole-5-carboxylate derivatives were designed and synthesized, and their SHP2 inhibitory activities (IC50) were determined. Among the desired compounds, 1d shares the highest inhibitory activity (IC50 = 0.99 µM) against SHP2. Additionally, a common feature pharmacophore model was established to explain the structure activity relationship of the desired compounds. Finally, molecular dynamics simulation was carried out to explore the most likely binding mode of compound 1d with SHP2. In brief, the findings reported here may at least provide a new strategy or useful insights in discovering novel effective SHP2 inhibitors.

5.
Virol J ; 17(1): 15, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32005266

RESUMO

BACKGROUND: Hantaan virus (HTNV) can cause hemorrhagic fever with renal syndrome (HFRS) in humans with severe morbidity and high mortality. Although inactivated HFRS vaccines are given annually for prevention in populations, China still has the highest number of HFRS cases and deaths worldwide. Consequently, vaccination for HFRS requires the development of novel, more effective vaccines. Epitope peptide vaccines have been developed rapidly in recent years and are considered a novel approach for the prevention of infection. Specifically, the multiple antigenic peptide (MAP) design with preferable immunogenicity can arouse a satisfactory immune response for vaccination. However, there are few reports on the design and evaluation of MAP for HTNV. METHODS: Three HLA-A*02-restricted 9-mer cytotoxic T lymphocyte (CTL) epitopes on HTNV glycoprotein and one HLA-A*02-restricted 9-mer CTL epitope on the HTNV nucleocapsid, which have been proven to be immunoprotective in our previous study, were selected for the design of HTNV MAP. A four-branched HTNV MAP was evaluated by the IFN-γ-secreting enzyme-linked immunospot assay and proliferation induction capacity of CD8+ T cells and compared with the single HTNV CTL epitope in 17 HLA-A*02+ patients with HFRS. The Mann-Whitney U test was used for comparison of parameters between different subject groups. RESULTS: The macromolecular HTNV MAP was designed with a polylysine core and four radially branched single CTL epitope chains. Importantly, HTNV MAP could stimulate CD8+ T cell secretion of IFN-γ in HLA-A*02+ patients with HFRS. The frequency of IFN-γ-secreting CD8+ T cells in the MAP stimulation group was significantly higher than that in the single HTNV CTL epitope stimulation groups (P < 0.005). Meanwhile, the activity of IFN-γ-secreting CD8+ T cells in the HTNV MAP group was also higher than that of the single CTL epitope groups (P < 0.05). Moreover, there was a much stronger ability of HTNV MAP to stimulate CD8+ T cell proliferation compared with that of a single HTNV CTL epitope. CONCLUSIONS: The designed HTNV MAP could induce CTL responses ex vivo and may be considered a candidate for the design and development of novel HTNV peptide vaccines.

6.
Nat Prod Res ; : 1-10, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32037886

RESUMO

Sanguisorba officinalis L. is a traditional herbal plant that belongs to the genus Sanguisorba and the family Rosaceae. Two new phenolic glycosides (1-2), ten known phenolics (3-12), and six known monoterpenoid glycosides (13-18) were isolated from the roots of S. officinalis using silica gel column and preparative middle pressure liquid chromatography (MPLC). The chemical structures were elucidated based on extensive spectroscopic experiments, including 1D and 2D NMR as well as HR-ESI-MS, and comparison with those reported in the literature. Compounds 3-5, and 13 were isolated from the Rosaceae family and compound 7 was obtained from the genus Sanguisorba for the first time. Additionally, all compounds were evaluated for their anti-complementary activities against the classical pathway. Furthermore, compounds 1, 5, 9, and 14 showed significant anti-complementary activities with the 50% haemolytic inhibition concentrations (CH50) values of 0.40 ± 0.03, 0.57 ± 0.01, 0.51 ± 0.07, and 0.53 ± 0.05 mM, respectively.

7.
Int J Biol Macromol ; 150: 459-467, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32057866

RESUMO

Precise prediction and measurement of dispersibility of silver nanoparticles (AgNPs) under atmospheric conditions are extremely vital for their potential commercial application. In the present work, the dispersibility of AgNPs capped by polysaccharide-protein from viscera of abalone (PSP-AgNPs) was studied using the combination of ultraviolet-visible spectroscopy (UV-vis), dynamic light scattering (DLS) and multiple-light-scattering (MLS) techniques. The results showed that the combination of UV/vis, DLS and MLS not only accurately determined the dispersibility of PSP-AgNPs, but also provided detailed information about the aggregation behavior of PSP-AgNPs. Furthermore, the results revealed a high dispersibility of PSP-AgNPs in the studied environment. The system temperature, pH value and thermal treatment (pasteurization and sterilization) had no effect on the dispersion of PSP-AgNPs in the effective concentration range against the pathogenic bacteria. Besides, an excellent stable dispersion and antibacterial activity against common pathogenic vibrio was also found in the dispersed PSP-AgNPs in seawater. Overall, the study provides a suitable method for the precise measurement of the dispersibility of AgNPs in environment. The AgNPs act as a potential bactericide with good dispersion and antibacterial activity in mariculture and other fields.

8.
J Exp Clin Cancer Res ; 39(1): 17, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31952546

RESUMO

BACKGROUND: STAT5 plays an important role in the transformation of hematopoietic cells by BCR-ABL. However, the downstream target genes activated by STAT5 in chronic myeloid leukemia (CML) cells remain largely unclear. Here, we investigated the mechanistic functional relationship between STAT5A-regulated microRNA and CML cell apoptosis. METHODS: The expression of USP15, Caspase-6, STAT5A-regulated miR-202-5p and STAT5A was detected by qRT-PCR and Western blotting in CML cell lines and PBMCs of CML patients. Cell apoptosis was evaluated by flow cytometry. Both gain- and loss-of-function experiments were used to investigate the roles of USP15, miR-202-5p and STAT5A in CML. Luciferase reporter assay detected the effect of miR-202-5p on USP15 expression. Xenograft animal model was used to test the effect of anti-miR-202-5p and pimozide on K562 cell xenograft growth. RESULTS: USP15 expression was significantly downregulated in CML cell lines and PBMCs of CML patients. Depletion of USP15 increased, whereas overexpression of USP15 reduced the resistance of CML cells to Imatinib. Further, decreased deubiquitinating activity of USP15 by USP15 downregulation led to reduced caspase-6 level, thus attenuating CML cell apoptosis. Mechanistically, miR-202-5p was upregulated in K562G cells and negatively regulated USP15 expression by directly targeting USP15 3'-UTR. Correspondingly, upregulation of miR-202-5p enhanced the resistance of CML cells to Imatinib by inhibiting cell apoptosis. Importantly, STAT5A was upregulated in CML cells and directly activated miR-202-5p transcription by binding to the pre-miR-202 promoter. Pimozide induced CML cell apoptosis and significantly reduced K562 cell xenograft growth in vivo by blocking STAT5A/miR-202-5p/USP15/Caspase-6 regulatory axis. CONCLUSIONS: we provide the first evidence that de-regulated STAT5A/miR-202-5p/USP15/Caspase-6 regulatory axis suppresses the apoptosis of CML cells, targeting this pathway might be a promising therapeutic approach for the treatment of CML.

9.
Psychiatr Q ; 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31925625

RESUMO

The objective of this study was to investigate the correlation of tinnitus severity and sleep quality prior to tinnitus onset in a Chinese population.We recruited patients with primary tinnitus from a tertiary teaching hospital in southwest China, retrospectively. The Pittsburgh Sleep Quality Index (PSQI) and the Mandarin version of the Tinnitus Handicap Inventory (THI-M) were employed to assess tinnitus severity and sleep quality of past, respectively. A battery of hearing tests was also administered to subjects, including TEOAE, pure tone audiometry, and tympanometry, for hearing evaluation.We enrolled 190 patients and nine were excluded. Subjects were divided into two groups: group A (PSQI <7) and group B (PSQI ≥7). The mean duration of tinnitus in both groups was above 6 months. There was a significant difference between THI-M global scores of group A and group B (P < 0.001). The difference in tinnitus severity ranks between the two groups was also significant (P = 0.006). The proportion of severe tinnitus levels in group B was higher than that of group A. Spearman's correlation analysis did not show correlation between the scores of THI-M and that of the PSQI in group A (P = 0.077); in verse, a positive correlation between THI-M and PSQI scores was found in group B (P < 0.001).The tinnitus severity is positively correlated with sleep quality before tinnitus onset, suggesting that the sleep quality of the past may have an impact on tinnitus occurrence.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31961653

RESUMO

Conductive coatings show great promise for next-generation electromagnetic interference (EMI) shielding challenges on textile; however, their stringent requirements for electrical conductivity are difficult to meet by conventional approaches of increasing the loading and homogeneity of conductive nanofillers. Here, the axial alignment of carbon nanotubes (CNTs) on fibers that were obtained by spontaneous capillary-driven self-assembly is shown on commercial cotton fabrics, and its great potential for EMI shielding is demonstrated. The aligned CNTs structurally optimize the conductive network on fabrics and yield an 81-fold increase in electrical conductivity per unit of CNT, compared with the disordered CNT microstructure. The high-efficiency electrical conductivity allows a several-micron-thick coating on insulating fabrics to endow an EMI shielding effectiveness of 21.5 dB in the X band and 20.8 dB in the Ku band, which meets the standard shielding requirement in commercial applications. It is among the minimum reported thicknesses for conductive nanocomposite coatings to date. Moreover, the coated fabrics with aligned CNTs possess a desirable stability upon bending, scratching, stripping, and even washing, which is attributed to the dense CNT packing in the aligned microarchitecture. This work presents the anisotropic structure on large areas by self-assembly, offering new opportunities for next-generation portable and wearable electronic devices.

11.
J Am Chem Soc ; 142(1): 290-299, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31801348

RESUMO

Polymer-stabilized liquid/liquid interfaces are an important and growing class of bioinspired materials that combine the structural and functional capabilities of advanced synthetic materials with naturally evolved biophysical systems. These platforms have the potential to serve as selective membranes for chemical separations and molecular sequencers and to even mimic neuromorphic computing elements. Despite the diversity in function, basic insight into the assembly of well-defined amphiphilic polymers to form functional structures remains elusive, which hinders the continued development of these technologies. In this work, we provide new mechanistic insight into the assembly of an amphiphilic polymer-stabilized oil/aqueous interface, in which the headgroups consist of positively charged methylimidazolium ionic liquids, and the tails are short, monodisperse oligodimethylsiloxanes covalently attached to the headgroups. We demonstrate using vibrational sum frequency generation spectroscopy and pendant drop tensiometery that the composition of the bulk aqueous phase, particularly the ionic strength, dictates the kinetics and structures of the amphiphiles in the organic phase as they decorate the interface. These results show that H-bonding and electrostatic interactions taking place in the aqueous phase bias the grafted oligomer conformations that are adopted in the neighboring oil phase. The kinetics of self-assembly were ionic strength dependent and found to be surprisingly slow, being composed of distinct regimes where molecules adsorb and reorient on relatively fast time scales, but where conformational sampling and frustrated packing takes place over longer time scales. These results set the stage for understanding related chemical phenomena of bioinspired materials in diverse technological and fundamental scientific fields and provide a solid physical foundation on which to design new functional interfaces.

12.
Anal Chem ; 92(1): 740-748, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31750649

RESUMO

The dynamics of mitochondria in live cells play a pivotal role in biological events such as cell metabolism, early stage apoptosis, and cell differentiation. Triphenylphosphonium (TPP) is a commonly used mitochondria-targeting agent for mitochondrial studies. However, there has been a lack of understanding in intracellular behaviors of TPP in the course of targeting mitochondria due to the difficulty in tracking and quantifying small molecules in a biological environment. Here, we report the utility of hyperspectral stimulated Raman scattering (SRS) microscopy associated with a Raman tag synthesized for real-time visualization and quantitation of TPP dynamics within live cells at the subcellular level. With the myriad of merits offered by a synthesized aryl-diyne-based Raman tag such as excellent photostability, negligible background interferences, and a linear dependence of the SRS signal on the TPP concentration, we successfully establish a quantitative model to associate the mitochondrial membrane potential with the key pharmacokinetic parameters of TPP inside the live cells. The model reveals that reduction in the mitochondrial membrane potential leads to significant decreases in both the uptake rate and intracellular concentrations of TPP. Further, on the basis of the multiplexed SRS images concurrently highlighting the cellular proteins and lipids without further labeling, we find that the TPP uptake causes little cytotoxicity to the host cells. The bioorthogonal hyperspectral SRS microscopy imaging reveals that TPP can maintain stable affinity to mitochondria during the restructuring of mitochondrial networking, demonstrating its great potential for real-time monitoring of pharmacokinetics of small molecules associated with live biological hosts, thereby promoting the development of mitochondria-targeting imaging probes and therapies in the near future.

13.
J Sci Food Agric ; 100(3): 1092-1098, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31667839

RESUMO

BACKGROUND: Plant-growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal (AM) fungi have the ability to enhance the growth, fitness, and quality of various agricultural crops, including cowpea. However, field trials confirming the benefits of microbes in large-scale applications using economically viable and efficient inoculation methods are still scarce. Microbial seed coating has a great potential for large-scale agriculture through the application of reduced amounts of PGPR and AM fungi inocula. Thus, in this study, the impact of seed coating with PGPR, Pseudomonas libanensis TR1 and AM fungus, Rhizophagus irregularis (single or multiple isolates) on grain yield and nutrient content of cowpea under low-input field conditions was evaluated. RESULTS: Seed coating with P. libanensis + multiple isolates of R. irregularis (coatPMR) resulted in significant increases in shoot dry weight (76%), and in the number of pods and seeds per plant (52% and 56%, respectively) and grain yield (56%), when compared with non-inoculated control plants. However, seed coating with P. libanensis + R. irregularis single-isolate (coatPR) did not influence cowpea grain yield. Grain lipid content was significantly higher (25%) in coatPMR plants in comparison with control. Higher soil organic matter and lower pH were observed in the coatPMR treatment. CONCLUSIONS: Our findings indicate that cowpea field productivity can be improved by seed coating with PGPR and multiple AM fungal isolates under low-input agricultural systems. © 2019 Society of Chemical Industry.


Assuntos
Produção Agrícola/métodos , Glomeromycota/fisiologia , Pseudomonas/fisiologia , Sementes/microbiologia , Vigna/crescimento & desenvolvimento , Micorrizas/fisiologia , Sementes/crescimento & desenvolvimento , Solo/química , Vigna/microbiologia
14.
Biol Trace Elem Res ; 194(1): 76-83, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31147977

RESUMO

Previous studies have reported an association between blood lead (BPb) levels and depressive symptoms in adults. However, the relationship between BPb levels and depression remains unclear in the old population. Our purpose was to investigate the association of BPb levels with depression among the old population. Data were gathered from 994 elderly persons in the Cohort Study of Elderly Health and Environmental Controllable Factors, which was created in Anhui, China. BPb was measured by inductively coupled plasma mass spectrometry. Our subjects were divided into four groups according to BPb quartiles. Multivariate logistic regressions were used to assess the association between BPb levels and depression. The median (25th-75th) BPb level was 26.94 µg/L (20.34 µg/L-36.13 µg/L). BPb levels in participants with depression were higher than those in participants without depression. The prevalence of depression was 27.64%. After multivariate adjustment for confounding factors, participants with BPb level in the highest quartile had 2.03 times higher risk of depression compared with those in the lowest quartile. In rural areas, the risk of depression in higher BPb quartile was higher than that in the lowest quartile. These findings suggest that higher BPb level was associated with increased odds of depression in the old population. This association was obvious among older people living in rural areas.

15.
Eur Radiol ; 30(2): 1254-1263, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31468159

RESUMO

OBJECTIVE: To investigate the discriminative capabilities of different machine learning-based classification models on the differentiation of small (< 4 cm) renal angiomyolipoma without visible fat (AMLwvf) and renal cell carcinoma (RCC). METHODS: This study retrospectively collected 163 patients with pathologically proven small renal mass, including 118 RCC and 45 AMLwvf patients. Target region of interest (ROI) delineation, followed by texture feature extraction, was performed on a representative slice with the largest lesion area on each phase of the four-phase CT images. Fifteen concatenations of the four-phasic features were fed into 224 classification models (built with 8 classifiers and 28 feature selection methods), classification performances of the 3360 resultant discriminative models were compared, and the top-ranked features were analyzed. RESULTS: Image features extracted from the unenhanced phase (UP) CT image demonstrated dominant classification performances over features from other three phases. The two discriminative models "SVM + t_score" and "SVM + relief" achieved the highest classification AUC of 0.90. The 10 top-ranked features from UP included 1 shape feature, 5 first-order statistics features, and 4 texture features, where the shape feature and the first-order statistics features showed superior discriminative capabilities in differentiating RCC vs. AMLwvf through the t-SNE visualization. CONCLUSION: Image features extracted from UP are sufficient to generate accurate differentiation between AMLwvf and RCC using machine learning-based classification model. KEY POINTS: • Radiomics extracted from unenhanced CT are sufficient to accurately differentiate angiomyolipoma without visible fat and renal cell carcinoma using machine learning-based classification model. • The highest discriminative models achieved an AUC of 0.90 and were based on the analysis of unenhanced CT, alone or in association with images obtained at the nephrographic phase. • Features related to shape and to histogram analysis (first-order statistics) showed superior discrimination compared with gray-level distribution of the image (second-order statistics, commonly called texture features).

16.
Brain Behav ; 10(1): e01496, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31828968

RESUMO

INTRODUCTION: The association between preceding infection of hepatitis E virus (HEV) and Guillain-Barre syndrome (GBS) has been found for more than a decade, while hepatitis E virus-associated Guillain-Barre syndrome (HEV-associated GBS) still remains poorly understood. Initially discovered in 2000, the association between GBS and HEV has been focused by neurologists increasingly. Five percent of patients with GBS had preceding acute HEV infection in the Netherlands and higher rate was found in Bangladesh (11%) where HEV is endemic. METHOD: An extensive review of relevant literature was undertaken. RESULTS: Hepatitis E virus infection may induce GBS via direct viral damage according to recent research findings. On the other hand, the presence of antiganglioside GM1 or GM2 antibodies in serum of some HEV-associated GBS patients indicates that HEV infection may trigger GBS by activating autoimmune response to destroy myelin or axon mistakenly. Management of HEV-associated GBS has no obvious difference from GBS. It mainly consists of supportive therapy and immunotherapy. Intravenous immunoglobulin (IVIG) or plasma exchange (PLEX) was used in most reported cases, which is the main strategy for clinical treatment of HEV-associated GBS. Whether antiviral therapy could be additional strategy other than the routine therapy to shorten the length of disease course is one of the most urgent problems and requires further study. CONCLUSIONS: An overview of possible pathogenesis will gain a first insight into why HEV, traditionally recognized as only hepatotropic, can induce many neurological disorders represented by GBS. Moreover, understanding of the underlying mechanisms may contribute to development of a novel therapeutic strategy. This review also summarizes management and clinical characteristics of HEV-associated GBS, aiming to achieve early recognition and good recovery.

17.
Nat Prod Res ; 34(4): 518-524, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30470127

RESUMO

A new secoiridoid, (1R,5S,8S,9R)-1-methyl-kingiside aglucone (1), along with nine known compounds (2-10), were isolated from the ethanol extract of the stem bark of Syringa reticulata (Bl.) Hara. The structure of compound 1 was elucidated using HR-ESI-MS, 1D and 2D NMR spectroscopy. Compounds 1-10 were evaluated for their inhibitory activity against NO, TNF-α and IL-6 production. Compounds 1, 3, 5 and 7-10 significantly inhibited the levels of NO, TNF-α and IL-6 in LPS-induced RAW264.7 cells from concentrations of 3 to 30 µM.

18.
ACS Nano ; 14(1): 620-631, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31877023

RESUMO

Combination therapy that could better balance immune activation and suppressive signals holds great potential in cancer immunotherapy. Herein, we serendipitously found that the pH-responsive nanovesicles (pRNVs) self-assembled from block copolymer polyethylene glycol-b-cationic polypeptide can not only serve as a nanocarrier but also cause immunogenic cell death (ICD) through preapoptotic exposure of calreticulin. After coencapsulation of a photosensitizer, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a (HPPH) and an indoleamine 2,3-dioxygenase inhibitor, indoximod (IND), pRNVs/HPPH/IND at a single low dose elicited significant antitumor efficacy and abscopal effect following laser irradiation in a B16F10 melanoma tumor model. Treatment efficacy attributes to three key factors: (i) singlet oxygen generation by HPPH-mediated photodynamic therapy (PDT); (ii) increased dendritic cell (DC) recruitment and immune response provocation after ICD induced by pRNVs and PDT; and (iii) tumor microenvironment modulation by IND via enhancing P-S6K phosphorylation for CD8+ T cell development. This study exploited the nanocarrier to induce ICD for the host's immunity activation. The "all-in-one" smart nanovesicles allow the design of multifunctional materials to strengthen cancer immunotherapy efficacy.

19.
Plant Dis ; 104(2): 330-339, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31850823

RESUMO

The utility of traditional methods for detecting seed-borne fungi is limited by the fact some fungi are unculturable or difficult to isolate. The seed-borne pathogens affecting Panax ginseng cultivation have not been fully characterized. Seed-borne fungi can be identified based on the high-throughput sequencing of internal transcribed spacer (ITS) amplicons. A hierarchical clustering tree diagram analysis based on operational taxonomic units revealed a relationship between the seed-borne fungi and the region from which the seeds were collected. This study analyzed the fungal diversity on 30 ginseng seed samples from the main ginseng-producing areas of China. The 50 most abundant genera were identified including those responsible for ginseng diseases, Fusarium, Alternaria, Nectria, Coniothyrium, Verticillium, Phoma, and Rhizoctonia. Fusarium species, which are the primary causes of root rot, were detected in all seed samples. The results of a phylogenetic analysis indicated that the seed-borne fungal species originating from the same region were closely related. Fungi on ginseng seeds from eight different regions were divided into eight clades, suggesting they were correlated with the local storage medium. A total of 518 Fusarium isolates were obtained and 10 species identified, all of which can be detrimental to ginseng production. Pathogenicity tests proved that seed-borne Fusarium species can infect ginseng seedlings and 2-year-old ginseng root, with potentially adverse effects on ginseng yield and quality.

20.
Biol Chem ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31863691

RESUMO

Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been identified as a regulatory molecule in angiogenesis. The goal of this study was to illustrate how MEG3 affects the angiogenesis of vascular endothelial cells. Expression of MEG3, miR-147 and intracellular cell adhesion molecule-1 (ICAM-1) in human microvascular endothelial cell line (HMEC-1) was altered by transfection, then cell viability, apoptosis, migration, tube formation, as well as the correlation among MEG3, miR-147 and ICAM-1 were explored. MEG3 was down-regulated during tube formation of HMEC-1 cells. MEG3 expression suppressed cells viability, migration and tube formation, while it induced apoptosis. MEG3 could bind with miR-147 and repress miR-147 expression. MiR-147 induced ICAM-1 expression, and contained ICAM-1 target sequences. The anti-atherogenic actions of MEG3 were inhibited by miR-147, and the anti-atherogenic actions of miR-147 suppression were also inhibited when ICAM-1 was overexpressed. Further, ICAM-1 overexpression showed activated roles in Wnt/ß-catenin and Jak/Stat signaling pathways. In low-density lipoprotein receptor (Ldlr)-/- mice, MEG3 overexpression reduced CD68+, CD3+ and ICAM-1 areas in lesions and increased collagen content. MEG3 inhibited HMEC-1 cell growth, migration and tube formation. The anti-atherogenic actions of MEG3 might be mediated via sponging miR-147, and thereby repressing the expression of ICAM-1.

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