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1.
Chem Biodivers ; : e202400330, 2024 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-38701178

RESUMO

Fungal infections represent a serious health problem worldwide. The study evaluated the antifungal activity of 4-chlorobenzyl p-coumarate, an unprecedented semi-synthetic molecule. Docking molecular and assay experiments were conducted to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC), mode of action, effect on growth, fungal death kinetics, drug association, effects on biofilm, micromorphology, and against human keratinocytes. The investigation included 16 strains of Candida spp, including C. albicans, C. krusei, C. glabrata, C. tropicalis, C. dubliniensis, C. lusitaniae, C. utilis, C. rugosa, C. guilhermondi, and C. parapsilosis. Docking analysis predicted affinity between the molecule and all tested targets. MIC and MFC values ranged from 3.9 µg/mL (13.54 µM) to 62.5 µg/mL (217.01 µM), indicating a probable effect on the plasma membrane. The molecule inhibited growth from the first hour of testing. Association with nystatin proved to be indifferent. All concentrations of the molecule reduced fungal biofilm. The compound altered fungal micromorphology. The tested compound exhibited an IC50 of 7.90 ± 0.40 µg/mL (27.45 ± 1.42 µM) for keratinocytes. 4-chlorobenzyl p-coumarate showed strong fungicidal effects, likely through its action on the plasma membrane and alteration of fungal micromorphology, and mildly cytotoxic to human keratinocytes.

2.
Clin Oral Investig ; 26(7): 4825-4833, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35305150

RESUMO

OBJECTIVE: To advance studies on the effect of a new pharmaceutical formulation for the treatment of oral fungal infections, we evaluated the safety and tolerability of orabase ointment containing cinnamaldehyde for use on the oral mucosa. MATERIAL AND METHODS: A clinical trial (phase I) was carried out on 35 individuals with healthy oral mucosa divided into three groups: ointments at 200 µg/mL, n = 12; 300 µg/mL, n = 11; and 400 µg/mL, n = 12. Product safety was assessed using three parameters: (a) clinical evolution as recorded by trained examiners; (b) evolution of the inflammatory process as registered by an exfoliative cytology exam and analyzed by trained pathologists; (c) mucosal swab to count Candida spp. colony-forming units (CFU). These parameters were analyzed both beforehand and at 15 days of treatment. RESULTS: The three ointment concentrations evaluated did not trigger inflammatory processes. The mycological analyses revealed a reduction of at least 99% in the number of Candida spp. CFU. In the exfoliative cytology analyses, the cells were found to be healthy. Participants reported a pleasant taste, yet 17% reported a slight burning sensation when applying the product. CONCLUSIONS: The ointment is safe and tolerable for use on healthy oral mucosa. TRIAL REGISTRATION: Registration number: RBR-7zwzs3. CLINICAL RELEVANCE: The ointment proved to be safe and tolerable for use on oral mucosa, encouraging studies to evaluate its clinical efficacy in patients with oral candidiasis, and contributing to a new therapeutic proposal for the treatment of fungal infections caused by Candida spp.


Assuntos
Candidíase Bucal , Micoses , Acroleína/análogos & derivados , Antifúngicos/farmacologia , Candida , Candidíase Bucal/tratamento farmacológico , Candidíase Bucal/microbiologia , Carboximetilcelulose Sódica/análogos & derivados , Humanos , Micoses/tratamento farmacológico , Pomadas/farmacologia
3.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 215-225, may. 2021. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1342813

RESUMO

This review describes the geographical distribution, botanical data, popular use, chemical composition, pharmacological activities and genetic aspects related to Eugenia luschnathiana, a native Brazilian plant popularly known as "bay pitomba". E. luschnathiana leaves are characterized morphologically by the presence of a petiole, an attenuated base, acuminated apex, elliptical shape, and parallel venation. The major chemical compounds found in E. luschnathiana are sesquiterpenes. Literature reports showed that E. luschnathiana extracts have antioxidant properties and antimicrobial activity against Gram-negative and Gram-positive bacteria. The extractsfrom the leaf, fruit and stem, and a concentrated residual solution of its essential oil, displayed negligible toxicity. Lastly, a cytogenetic analysis indicated that some markers can be used for the study of genetic diversity, population structure, and genetic improvements. The information available on E. luschnathiana supports the hypothesis that this plant may be a source of compounds with promising pharmacological activity.


Esta revisión describe la distribución geográfica, datos botánicos, uso popular, composición química, actividad farmacológica y el análisis genético de Eugenia luschnathiana, una planta originaria del Brasil conocida popularmente como "pitomba da baía". Las hojas de E. luschnathiana se caracterizan por la presencia de pecíolo, base atenuada, ápice acuminado, forma elíptica y venación paralela. Su composición química presenta mayormente sesquiterpenos. Los informes en la literatura muestran que los extractos de E. luschnathiana presentan propiedades antioxidantes y actividad antimicrobiana contra las bacterias Gram-negativas y Gram-positivas. Los extractos de la hoja, fruto y tallo, y una solución residual concentrada del aceite esencial, presentaron baja toxicidad. Por último, un análisis citogenético indicó que algunos marcadores pueden utilizarse para estudios de diversidad genética, estructura poblacional y mejoramiento genético. Las informaciones disponibles acerca de E. luschnathiana proponen la hipótesis de que esta planta puede ser una fuente de compuestos con actividad farmacológica prometedora.


Assuntos
Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Eugenia/química , Anti-Infecciosos/farmacologia , Terpenos/análise , Bactérias/efeitos dos fármacos , Óleos Voláteis/química , Extratos Vegetais/genética , Extratos Vegetais/química , Folhas de Planta/química , Eugenia/genética , Medicina Tradicional , Anti-Infecciosos/química
4.
Biomed Res Int ; 2021: 6653311, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33880374

RESUMO

INTRODUCTION: The absence of a standardized classification scheme for the antifungal potency of compounds screened against Candida species may hinder the study of new drugs. This systematic review proposes a scheme of interpretative breakpoints for the minimum inhibitory concentration (MIC) of bioactive compounds against Candida species in in vitro tests. MATERIALS AND METHODS: A literature search was conducted in the PubMed, Scopus, Web of Science, Lilacs, and SciFinder databases for the period from January 2015 to April 2020. The following inclusion criterion was used: organic compounds tested by the microdilution technique according to the Clinical and Laboratory Standards Institute protocol against reference strains of the genus Candida. A total of 545 articles were retrieved after removing duplicates. Of these, 106 articles were selected after applying the exclusion criteria and were evaluated according to the number of synthesized molecules and their chemical classes, the type of strain (reference or clinical) used in the antifungal test, the Candida species, and the MIC (in µg/mL) used. RESULTS: The analysis was performed based on the median, quartiles (25% and 75%), maximum, and minimum values of four groups: all strains, ATCC strains, C. albicans strains, and C. albicans ATCC strains. The following breakpoints were proposed to define the categories: MIC < 3.515 µg/mL (very strong bioactivity); 3.516-25 µg/mL (strong bioactivity); 26-100 µg/mL (moderate bioactivity); 101-500 µg/mL (weak bioactivity); 500-2000 µg/mL (very weak bioactivity); and >2000 µg/mL (no bioactivity). CONCLUSIONS: A classification scheme of the antifungal potency of compounds against Candida species is proposed that can be used to identify the antifungal potential of new drug candidates.


Assuntos
Antifúngicos/análise , Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Bibliometria , Humanos , Testes de Sensibilidade Microbiana
5.
Artigo em Português | LILACS | ID: biblio-1525576

RESUMO

Introdução: A doença cárie possui uma etiologia multifatorial que pode atingir toda a estrutura dentária. Em cavidades profundas, o tratamento expectante pode ser realizado com a finalidade de evitar danos à polpa. Já em lesões iniciais, a técnica ART é uma alternativa aceitável para o caso. Objetivo: Discutir sobre as indicações, contraindicações, técnicas e relevâncias clínicas do tratamento expectante (convencional e modificado) e da técnica restauradora atraumática (ART) no tratamento das lesões cariosas. Métodos: Foi realizada uma revisão da literatura de artigos científicos publicados nas bases de dados PubMED/Medline e Scielo, entre 2009 e 2020, e de livros considerados relevantes para este estudo. Resultados: No tratamento expectante, a remoção da dentina cariada é realizada em duas etapas: remoção da lesão cariosa de forma superficial, na primeira consulta, e remoção final após diferentes intervalos de tempo. No entanto, alguns autores defendem que não é necessária a realização da segunda etapa, basta apenas rebaixar o cimento de ionômero de vidro restaurador e, em seguida, realizar a restauração definitiva, técnica nomeada como tratamento expectante modificado. Com relação à técnica ART, o manejo da lesão é feito com a mínima intervenção possível, utilizando apenas instrumentos manuais. É confiável e bem aceita, principalmente na clínica de Odontopediatria. Ambas culminam em resultados terapêuticos favoráveis. Conclusão: Inicialmente, o dentista deve sempre realizar um diagnóstico minucioso das lesões de cárie, visando a máxima prevenção da estrutura dental. Devemos optar pelo tratamento restaurador convencional somente quando outros métodos não forem mais suficientes para interromper a progressão da lesão.


Introduction: Caries disease has a multifactorial etiology and can affect the entire tooth structure. In deep cavities, expectant treatment can avoid damage to the pulp. In early lesions, the ART technique is an acceptable alternative. Objective: To discuss indications, contraindications, techniques, and clinical relevance of the expectant treatment (conven-tional and modified) and the ART technique in the treatment of carious lesions. Methods: A literature review of scientific articles (published in PubMED/Medline and Scielo data-bases, between 2009 and 2020) and books considered relevant to this study. Results: In the expectant treatment, carious dentin is removed in two stages: a superficial removal of the carious lesion at the first visit, and a final removal after different time intervals. However, some authors argue that it is not necessary to perform the second step. Just lower the glass-ionomer cements and then perform the definitive restoration, a technique known as modified expectant treatment, is necessary. Regarding the ART technique, the management of the lesion is conducted with the least possible intervention, using only manual instruments. It is reliable and well accepted especially in the Pediatric Dentistry clinic. Both culminate in favorable therapeutic results. Conclusion: Initially, the dentist must always carry out a thorough diagnosis of caries lesions aiming at the maximum prevention of the dental structure. You should opt for conventional restorative treatment only when other methods are no longer sufficient to stop the progression of the lesion.


Assuntos
Cárie Dentária/prevenção & controle , Odontopediatria , Dentina
6.
Rev. Salusvita (Online) ; 38(3): 795-810, 2019.
Artigo em Português | LILACS | ID: biblio-1052125

RESUMO

Introdução: Um procedimento restaurador estético requer previsibilidade quanto ao seu resultado. Nesse sentido, vêm sendo elaboradas novas técnicas e instrumentos computadorizados que auxiliam o planejamento e permitem tanto ao clínico quanto ao paciente, ainda nas etapas que antecedem o tratamento restaurador, uma percepção mais clara do procedimento que será realizado. Objetivos: compreender melhor a importância da utilização do ensaio restaurador (mockup) e do planejamento digital por meio do programa Digital Smile Design (DSD), na obtenção de um sorriso harmonioso e previsível, ressaltando aspectos como a execução das técnicas, os materiais utilizados, bem como indicações, contra-indicações, vantagens e desvantagens. Métodos: Realizou-se um levantamento bibliográfico, com busca nas principais bases de dados PubMED/Medline, Lilacs e Scielo, por meio dos descritores Sorriso ("Smile"), Planejamento ("Planning"), Estética ("Aesthetic") e Design ("Design"), obtendo-se artigos de pesquisa, revisão, e ainda, relatos de caso, que se enquadravam nos critérios de inclusão previamente estabelecidos. Resultados: Ante o exposto, tornou-se clara a importância do DSD e mockup na busca por resultados cada vez mais satisfatórios e condizentes com as expectativas dos pacientes, permitindo ao clínico, maior previsibilidade durante a elaboração dos seus tratamentos; reduzindo o número de ajustes intra-orais e desgastes desnecessários; favorecendo ainda, uma melhor comunicação entre a equipe e os pacientes. Conclusão: Tanto o DSD quanto o mockup, são consideradas ferramentas indispensáveis para odontologia atual que vem exigindo do cirurgião- -dentista um trabalho cada vez mais minucioso e perfeccionista.


Introduction: An aesthetic restorative procedure requires predictability as to its result. In this sense, new techniques and computerized instruments are being developed, which help to plan and allow both the clinician and the patient, even in the stages that precede the restorative treatment, a clearer perception of the procedure that will be performed. Objectives: to better understand the importance of the use of the mockup and digital planning through the Digital Smile Design (DSD) program, in order to achieve a harmonious and predictable smile, highlighting aspects such as the implementation of the techniques, the materials used, as well as indications, contraindications, advantages and disadvantages. Methods: A bibliographic survey was carried out, with a search of the main PubMED / Medline, Lilacs and Scielo databases, using the descriptors Smile ("Smile"); Planning ("Planning"); Aesthetic ("Aesthetic") and Design ("Design"), obtaining articles of research, review, and also, case reports, that fit the inclusion criteria previously established. Results: The importance of DSD and mockup in the search for results that are increasingly satisfactory and in line with the expectations of the patients has become clear, allowing the clinician greater predictability during the elaboration of his treatments; reducing the number of adjustments intra-oral and unnecessary wear and tear; favoring a better communication between the team and patients. Conclusion: Both DSD and mockup are considered indispensable tools for current dentistry that has been requiring the dental surgeon to work more and more thoroughly and perfectionist.


Assuntos
Estética Dentária , Procedimentos Cirúrgicos Bucais
7.
Rev. Salusvita (Online) ; 37(4): 985-1007, 2018.
Artigo em Português | LILACS | ID: biblio-1050851

RESUMO

Introdução: atualmente, tanto no contexto literário quanto na prática, são muitos os procedimentos e testes utilizados para estabelecer o diagnóstico da condição pulpar dos dentes. Esses métodos de diagnóstico são de fundamental importância para o conhecimento da condição pulpar. Para tanto, o paciente deve passar por abordagens como a anamnese, exames físicos e complementares. Objetivo: discutir sobre os métodos utilizados para chegar a um correto diagnóstico pulpar, visando, posteriormente, um adequado tratamento. Métodos: foi realizada uma pesquisa bibliográfica nas principais bases de dados eletrônicos online, por meio da busca de artigos relacionados ao tema proposto, publicados entre o período de 2002 a 2018. Os descritores utilizados para seleção dos artigos foram: Endodontia; Diagnóstico; Teste; Anamnese; Pulpite. Dessa forma, foramselecionados para inclusão no estudo os trabalhos que obedeceram aos critérios de inclusão e exclusão. Conclusão: os testes são subdivididos em teste do frio, do calor, da cavidade, da anestesia, dentre outros. Tais testes, juntamente com a análise dos sinais e sintomas apresentados pelo paciente, permitem chegar a uma conclusão do estado em que a polpa se encontra, ou seja, se ela se apresenta patológica ou não, o que nos levará a decidir se devemos submetê-la ou não a determinados tipos de tratamentos.


Introduction: currently, both in the literary and in the practical context, there are many procedures and tests used to establish the diagnosis of the pulp condition of the teeth. These diagnostic methods are of fundamental importance for the knowledge of the pulp condition. To do so, the patient must undergo approaches such as anamnesis, physical and complementary exams. Objective: the present work aimed to discuss the methods used to arrive at a correct pulpal diagnosis, aiming at an appropriate treatment. Method: a bibliographic search was carried out in the main electronic databases online, through the search of articles related to the proposed theme, published between 2002 and 2018. The descriptors used to select the articles were: Endodontics; Diagnosis; Test; Anamnesis; Pulpit. In this way, the works that obeyed the inclusion criteria were selected. Conclusion: the tests are subdivided into tests of cold, heat, cavity, anesthesia, among others. Such tests, together with the analysis of the signs and symptoms presented by the patient, allow us to reach a conclusion on the state in which the pulp is found, that is, whether it is pathological or not, which will lead us to decide whether to submit it, or not to certain types of treatments.


Assuntos
Endodontia , Pulpite , Diagnóstico , Anamnese
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