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1.
Braz J Biol ; 83: e277489, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38126643

RESUMO

Cowpea is one of the main crops in family agriculture, especially in the Northeastern region of Brazil, and it is expanding to other regions in Brazil. The use of seeds with low physiological and health quality is reflected in the plant development and consequently yield, making it important to study the seed physiological and health quality. The objective of the present study was to assess the physiology and health of traditional and biofortified cowpea seeds. The traditional cowpea varieties (Angelim, Mercado and Manteguinha) and the biofortified cowpea cultivars (BRS Aracê, BRS Xique-Xique and BRS Tumucumaque) were assessed for the following physiological parameters: water content (WC), first count (FC), germination test (G), germination speed index (GSI), seedling emergence in the greenhouse (E), emergence speed index (ESI), seedling aerial part and root length (APL and RL) and electric conductivity test (EC). The seed health quality was assessed by the Blotter Test. The water content present in the seeds of the traditional and biofortified varieties ranged from 10% to 14%. All the traditional and biofortified varieties showed high germination and emergence value in the greenhouse. The germination and emergence speed indexes indicated the BRS Aracê and BRS Xique-Xique cultivars as the most vigorous. In the health tests the highest indexes were the storage fungi Aspergillus sp., Cladosporium sp. and Penicillium sp., with the highest prevalence in the BRS Tumucumaque variety, which was probably related to the higher water content present in this variety.


Assuntos
Vigna , Plântula , Germinação/fisiologia , Sementes , Água
2.
Braz. j. biol ; 83: 1-5, 2023. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1468828

RESUMO

Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), ɣ-terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.


Numerosos estudos têm investigado a composição química e as atividades biológicas de óleos essenciais extraídos de cascas dos frutos, folhas e flores de diferentes espécies de Citrus. Este trabalho tem como objetivo investigar a composição química e as atividades larvicida e leishmanicida in vitro do óleo essencial das cascas dos frutos de Citrus reticulata (CR-EO). CR-EO foi obtido pela técnica de extração em aparelho Clevenger e sua composição química foi determinada por CG-EM e CG-DIC. Limoneno (85,7%), ɣ-terpineno (6,7%) and mirceno (2,1%) foram identificados como os constituintes majoritários. CR-EO mostrou alta atividade contra as formas promastigota de Leishmania amazonensis (CI50 = 8,23 µg/mL). CR-EO também exibiu alta atividade larvicida contra as larvas do terceiro estágio do Aedes aegypti com concentração letal (CL50 = 58,35 µg/mL) e mortalidade de 100% em 150 µg/mL. Este estudo sugere, pela primeira vez, o uso potencial de CR-EO contra esta importante doença viral transmitida por mosquitos do gênero Aedes.


Assuntos
Aedes/efeitos dos fármacos , Citrus/química , Leishmania/efeitos dos fármacos , Limoneno/análise , Técnicas In Vitro , Óleos Voláteis/química
3.
Braz. j. biol ; 832023.
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469044

RESUMO

Abstract Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), -terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.


Resumo Numerosos estudos têm investigado a composição química e as atividades biológicas de óleos essenciais extraídos de cascas dos frutos, folhas e flores de diferentes espécies de Citrus. Este trabalho tem como objetivo investigar a composição química e as atividades larvicida e leishmanicida in vitro do óleo essencial das cascas dos frutos de Citrus reticulata (CR-EO). CR-EO foi obtido pela técnica de extração em aparelho Clevenger e sua composição química foi determinada por CG-EM e CG-DIC. Limoneno (85,7%), -terpineno (6,7%) and mirceno (2,1%) foram identificados como os constituintes majoritários. CR-EO mostrou alta atividade contra as formas promastigota de Leishmania amazonensis (CI50 = 8,23 µg/mL). CR-EO também exibiu alta atividade larvicida contra as larvas do terceiro estágio do Aedes aegypti com concentração letal (CL50 = 58,35 µg/mL) e mortalidade de 100% em 150 µg/mL. Este estudo sugere, pela primeira vez, o uso potencial de CR-EO contra esta importante doença viral transmitida por mosquitos do gênero Aedes.

4.
Braz. j. biol ; 83: e247539, 2023. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1278542

RESUMO

Abstract Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), ɣ-terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.


Resumo Numerosos estudos têm investigado a composição química e as atividades biológicas de óleos essenciais extraídos de cascas dos frutos, folhas e flores de diferentes espécies de Citrus. Este trabalho tem como objetivo investigar a composição química e as atividades larvicida e leishmanicida in vitro do óleo essencial das cascas dos frutos de Citrus reticulata (CR-EO). CR-EO foi obtido pela técnica de extração em aparelho Clevenger e sua composição química foi determinada por CG-EM e CG-DIC. Limoneno (85,7%), ɣ-terpineno (6,7%) and mirceno (2,1%) foram identificados como os constituintes majoritários. CR-EO mostrou alta atividade contra as formas promastigota de Leishmania amazonensis (CI50 = 8,23 µg/mL). CR-EO também exibiu alta atividade larvicida contra as larvas do terceiro estágio do Aedes aegypti com concentração letal (CL50 = 58,35 µg/mL) e mortalidade de 100% em 150 µg/mL. Este estudo sugere, pela primeira vez, o uso potencial de CR-EO contra esta importante doença viral transmitida por mosquitos do gênero Aedes.


Assuntos
Animais , Óleos Voláteis/farmacologia , Citrus , Aedes , Inseticidas/farmacologia , Frutas , Larva
5.
Braz J Biol ; 83: e247539, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34190766

RESUMO

Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), É£-terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.


Assuntos
Aedes , Citrus , Inseticidas , Óleos Voláteis , Animais , Frutas , Inseticidas/farmacologia , Larva , Óleos Voláteis/farmacologia
6.
Prev Vet Med ; 176: 104938, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32143028

RESUMO

Diseases that affect cattle represent obstacles to the development of livestock activity. Brucellosis is a significant such disease because it is transmissible, has a chronic nature, and causes health and economic damages to the herd and rural producer. Data from surveys performed in 2002 and 2014 were compared to identify the spatial distribution of bovine brucellosis and to evaluate clusters of outbreaks and areas of greater risk to have infected cattle in the state of Mato Grosso, Brazil. The present study analyzed the data obtained in the aforementioned investigations with a statistical model based on a spatial point process called a generalized additive model (GAM). The analysis made it possible to identify the regions of highest and lowest risk in the state of Mato Grosso. Of the 1001 properties analyzed in 2002, 198 were in areas with high-odds ratio, and 121 were in a low-odds ratio area. Of the 1248 properties sampled in 2014, 119 were in a high-odds ratio area, and 162 were in a low-odds ratio area. Areas with high-odds ratio are more likely to have infected cattle and can be considered to be at higher risk for the disease. The results of the present study highlight the reduction in foci, prevalence, and its relationship with the spatial distribution of bovine brucellosis. The study results should help the official defense service of Mato Grosso direct its activities according to the profile of each region.


Assuntos
Brucelose Bovina/epidemiologia , Surtos de Doenças/veterinária , Animais , Brasil/epidemiologia , Bovinos , Feminino , Masculino , Prevalência , Medição de Risco , Análise Espacial
7.
Public Health ; 171: 31-40, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31082758

RESUMO

OBJECTIVES: The aims of this study were (1) to identify attributes for patient safety at a primary healthcare level and (2) to analyze conceptions of patients, professionals, and managers about how these attributes are being addressed. STUDY DESIGN: This was a qualitative study. METHODS: Participants were recruited from three primary care settings in Brazil. A total of 37 subjects (four physicians, three nurses, three dentists, three managers, five community assistants, and 19 patients) participated on interviews about their perceptions of safety attributes at the primary care settings involved in the study. Some of these participants attended a focus group meeting. A thematic categorical analysis was carried out to interpret the interviews. RESULTS: The main attributes for patient safety were valued by the participants. However, barriers such as discontinuity of care, interruptions during consultations, breakdowns in the communication, and ineffective teamwork were reported as frequent sources of patient safety issues. Reports of patients left unattended for excessive time because of the lack of accurate information and disruptions that took up to 35 min show that there is still a long way to go for primary care to be safe and effective in the study settings. CONCLUSIONS: It is necessary that the strategies meet the patient safety needs more effectively and efficiently. Further research is needed to understand the complex nature of the problems that affect patient safety in these settings so that appropriate decisions can be made.


Assuntos
Determinação de Necessidades de Cuidados de Saúde , Segurança do Paciente , Atenção Primária à Saúde/organização & administração , Pessoal Administrativo/psicologia , Pessoal Administrativo/estatística & dados numéricos , Adulto , Brasil , Comunicação , Feminino , Pessoal de Saúde/psicologia , Pessoal de Saúde/estatística & dados numéricos , Humanos , Relações Interprofissionais , Masculino , Pessoa de Meia-Idade , Pesquisa Qualitativa , Adulto Jovem
8.
Int Endod J ; 52(4): 400-406, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30284719

RESUMO

AIM: To evaluate the frequency of post-treatment apical periodontitis associated with root filled teeth with at least one untreated root canal. METHODOLOGY: Eight hundred and seven cone beam computed tomography images containing at least one root filled tooth were selected from a collection of 1543 images from Brazilian individuals. Scans were taken using ICAT Classic devices (Imaging Sciences, Hatfield, PA, USA) in a private oral radiology clinic from January to April 2015. All root filled teeth were analysed for the presence of missed canals and apical periodontitis. The chi-square and odds ratio tests were used to verify if there were an association and risk relationship between the occurrence of untreated canals and apical periodontitis. RESULTS: A total of 2294 teeth with evidence of root fillings were identified. Two hundred and eighty-one teeth had at least one untreated missed canal (12%). The frequency of apical periodontitis in teeth with at least one untreated canal was significantly greater in comparison to teeth with all canals treated (274/281, 98% versus 1736/2013, 86%) (P < 0.01). The odds for apical periodontitis to be present was 6.25 times greater for teeth with an untreated canal. The mesiobuccal roots of maxillary first molars had the greatest frequency of untreated canals (114/154, 74%), with the second mesiobuccal canal being the most frequently missed (n = 106/114, 93%). CONCLUSION: Root filled teeth with at least one missed canal had a high prevalence of post-treatment apical periodontitis.


Assuntos
Periodontite Periapical , Brasil , Cavidade Pulpar , Humanos , Obturação do Canal Radicular , Raiz Dentária
9.
Arch Virol ; 162(3): 857-861, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27885560

RESUMO

Here we determined the relative expression of HERV-K and W proviruses in HIV infected and non-infected mothers as well as their respective babies up to 1 year-old. HIV-infected mothers, their babies and uninfected control groups presented expression of both HERV-K and HERV-W with relatively high frequency. While the level of HERV-K expression was similar among groups, the level of HERV-W expression in HIV-infected mothers was four-fold higher than the uninfected mothers from the control group (p < 0.01). HERV-W was down regulated in HIV-exposed babies in comparison to non-exposed babies. To our knowledge, this is the first report of HERV transcriptional activity in babies from 0-1 year-old.


Assuntos
Retrovirus Endógenos/isolamento & purificação , Infecções por HIV/transmissão , Infecções por HIV/virologia , HIV-1/isolamento & purificação , Doenças do Recém-Nascido/virologia , Adulto , Retrovirus Endógenos/classificação , Retrovirus Endógenos/genética , Feminino , HIV-1/classificação , HIV-1/genética , Humanos , Lactente , Recém-Nascido , Transmissão Vertical de Doenças Infecciosas , Masculino , Transcrição Gênica
10.
Theriogenology ; 82(5): 715-9, 2014 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-25037750

RESUMO

Recent work with P-36 demonstrates that the replacement of the last two doses of Follicle-Stimulating Hormone (FSH) with equine chorionic gonadotropin (eCG) increases embryo yields. However, it is unclear if the positive effect of eCG is related to its FSH-like activity, LH-like activity, or both. This study aimed to verify the replacement of eCG with pLH on the last day of superstimulatory treatment. Twenty-five Nelore cows were allocated to four groups: P-36 (control), P-36/eCG, P-36/LH2, and P-36/LH4. All animals underwent four treatments in a crossover design. The control group cows were superstimulated with decreasing doses of porcine Follicle-Stimulating Hormone (pFSH, 133 mg, im). In the P-36/eCG, P-36/LH2, and P-36/LH4 groups, the last two doses of pFSH were replaced in the former group by two doses of eCG (200 IU each dose, im) and in the latter two groups by two doses of pLH (1 and 2 mg each dose, im), respectively. Donors received fixed-time artificial insemination 12 and 24 hours after pLH. Embryo flushing was performed on D16. Data were analyzed by ANOVA (Proc Mixed, SAS). There was a trend of decreasing ovulation rate when comparing groups LH2 and eCG (P = 0.06). However, there was no significant difference in the mean number of viable embryos among groups P-36 (3.3 ± 0.7), P-36/eCG (4.5 ± 0.5), P-36/LH2 (3.7 ± 0.8), and P-36/LH4 (4.2 ± 1.0). It is concluded that the replacement of eCG by pLH on the last day of superstimulatory treatment can be performed with no significant variation in the production of viable embryos.


Assuntos
Bovinos/fisiologia , Gonadotropina Coriônica/farmacologia , Hormônio Luteinizante/farmacologia , Superovulação/efeitos dos fármacos , Animais , Gonadotropina Coriônica/administração & dosagem , Cloprostenol/administração & dosagem , Cloprostenol/farmacologia , Estradiol/administração & dosagem , Estradiol/farmacologia , Sincronização do Estro/efeitos dos fármacos , Sincronização do Estro/métodos , Feminino , Hormônio Foliculoestimulante/administração & dosagem , Hormônio Foliculoestimulante/farmacologia , Inseminação Artificial/veterinária , Hormônio Luteinizante/administração & dosagem , Progesterona/administração & dosagem , Progesterona/farmacologia
11.
Clin Microbiol Infect ; 19(1): E31-E43, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23167493

RESUMO

Human parvovirus B19V (B19V) has been associated with various haematological disorders, but data on its prevalence in leukaemia are scarce. In this cross-sectional study, we investigated patients in Sao Paulo, Brazil with leukaemia to determine the molecular frequency of B19 variants and characterize the viral genetic variability by partial and complete sequencing of the coding of non-structural protein 1 (NS1)/viral capsid proteins 1 and 2 (VP1/VP2). The presence of B19V infections was investigated by PCR amplification of the viral NS1 gene fragment and confirmed by sequencing analysis. The NS1/VP1/VP2 and partially larger gene fragments of the NS1-positive samples were determined by overlapping nested PCR and direct sequencing results. The B19V NS1 was detected in 40 (16%) of 249 bone marrow samples including 12/78 (15.4%) acute lymphoblastic leukaemia, 25/155 (16.1%) acute myeloid leukaemia and 3/16 (18.7%) chronic myeloid leukaemia samples. Of the 40 participants, 25 (62.5%) were infected with genotype 1a and 15 (37.5%) with genotype 3b. The phylogenetic analysis of other regions revealed that 12/40 (30%) of the patients with leukaemia were co-infected with genotypes 1a and 3b. In addition, a new B19V intergenotypic recombinant (1a/3b) and an NS1 non-recombinant genotype 1a were detected in one patient. Our findings demonstrated a relatively high prevalence of B19V monoinfections and dual infections and provide, for the first time, evidence of inter-genotypic recombination in adults with leukaemia that may contribute to the genetic diversity of B19V and may also be a source of new emerging viral strains with future implications for diagnosis, therapy and efficient vaccine development.


Assuntos
Leucemia/virologia , Infecções por Parvoviridae/complicações , Parvovirus B19 Humano/genética , Parvovirus B19 Humano/isolamento & purificação , Adolescente , Adulto , Idoso , Distribuição de Qui-Quadrado , Análise por Conglomerados , Coinfecção/virologia , Estudos Transversais , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Infecções por Parvoviridae/virologia , Filogenia , Reação em Cadeia da Polimerase , Estatísticas não Paramétricas , Proteínas não Estruturais Virais/genética , Adulto Jovem
12.
Braz. j. phys. ther. (Impr.) ; 7(1): 25-30, jan.-abr. 2003. ilus, tab
Artigo em Português | LILACS | ID: lil-344305

RESUMO

O objetivo deste estudo foi analisar o efeito do alongamento dos musculos da cadeia muscular inspiratoria, proposto pelo metodo de reeducacao postural global(RPG), sobre a forca muscular respiratoria e a expansibilidade toracica. Participaram deste estudo 20 voluntarios sadios, do sexo feminino, com idade entre 18 e 23 anos(media=21,2, DP=1,61), que foram divididos de forma randonizada em dois grupos: 10 voluntarios submetidos a postura `ra no chao com bracos fechados` por 20 minutos (grupo RPG) e 10 voluntarios que nao receberam o alongamento, permanecendo em repouso por 20 minutos(grupo controle). Utilizaram-se para a valiacao a medida das pressoes respiratorias maximas e a toracometria em ambos os grupos, antes e apos o periodo estabelecido. Observou-se que o metodo de alongamento utilizado proporcionou aumento da expansibilidade toracica e das pressoes respiratorias maximas, havendo significancia estatistica pelo metodo de Wilcoxon no que se refere a forca dos musculos inspiratorios no grupo que recebeu o alongamento. Tambem houve diferenca significativa na expansibilidade toracica da regiao axilar no grupo RPG quando comparado com o grupo controle. Os resultados sugerem investigar o efeito desse metodo de alongamento em pneumonopatias cronicos, os quais poderiam ser beneficiados, visando a sua utilizacao como recurso fisioterapeutico


Assuntos
Alongamento Ósseo , Manipulação da Coluna , Especialidade de Fisioterapia , Músculos Respiratórios
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