Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Pathogens ; 13(4)2024 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-38668258

RESUMO

Rabies, one of the most lethal global zoonoses, affects all mammals. It remains circulating worldwide in sylvatic cycles through terrestrial and airborne reservoirs, and in Brazil, bats are currently the main reservoirs and source of transmission. Wild boars, an important invasive alien species in Brazil, are a proven food source for hematophagous bats and may participate in the Brazilian sylvatic cycle of rabies. We evaluated the presence of this pathogen in hunted wild boars from the São Paulo state using histopathology, the direct fluorescent antibody test (DFA), viral isolation in cell culture (VICC), the rapid fluorescent focus inhibition test (RFFIT), and quantitative reverse transcription polymerase chain reaction (RT-qPCR). The results of histopathological, DFA, VICC, and RT-qPCR analysis were negative for all samples; seven serum samples tested positive in the RFFIT, and titers ranged from 0.13 IU/mL to 0.5 IU/mL. The presence of rabies virus-neutralizing antibodies in the studied wild boars suggests the circulation of the virus in these animals. Educative actions directed at hunters should include information on the prevention of this important zoonosis.

2.
Rev Bras Parasitol Vet ; 33(1): e016523, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38422329

RESUMO

Callithrix kuhlii is present in forest mosaics, edge habitats, and abandoned fields in the Atlantic Forest. In Bahia and Minas Gerais. This study aimed to identify helminths from C. kuhlii and relate them to the clinical data, weights, and indices of the liver and gonads. Necropsies were performed on 13 adult marmosets that were run over on the BA-001 highway. A principal component analysis (PCA) was conducted to describe the relationships between the variables investigated. Fifty-one helminths were collected from 30.77% (4/13) of the marmosets analyzed. Helminths were classified based on their morphological and morphometric characteristics. Primasubulura jacchi (Marcel, 1857), Platynosomum illiciens (Dougherty, 1946), and Prosthenorchis confusus (Dougherty, 1946) were the species identified, with prevalence rates of 7.69%, 7.69%, and 15.38%, respectively. In addition, this is a new host record of P. confusus. The two main axes of the PCA explained a high variability (PCA=67.7%), indicating reduced weight and indices of the organs of parasitized animals. This study expands the knowledge on parasites of C. kuhlii and its vulnerability to parasites, contributing to constructing an epidemiological profile of environmental health.


Assuntos
Callitrichinae , Helmintos , Animais , Callithrix/parasitologia , Brasil , Florestas
3.
Parasit Vectors ; 16(1): 449, 2023 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-38053206

RESUMO

BACKGROUND: Wild boars (Sus scrofa) may cause substantial damage to crops and can spread zoonotic parasites to domestic animals, posing a risk to health and animal production. Metastrongylus spp. can negatively affect the wild boar population, increasing piglet mortality. In addition to that, studies with Metastrongylus genetic characterization are still scarce in Brazil. The present study aims to characterize Metastrongylus spp. from wild boars hunted in the states of São Paulo, Paraná, and Rio Grande do Sul, Brazil, using traditional morphological description and DNA sequences in an integrative taxonomic approach. METHODS: After nematode collection from 58 wild boars, the parasites were morphologically identified and genetically characterized by the amplification of 18S ribosomal DNA (rDNA), 28S rDNA, internal transcribed spacer (ITS) region, and cox-1 mitochondrial DNA (mtDNA). Descriptors of infection were determined and Pearson's Chi-square test was applied to compare the prevalence of infections among the identified parasite species, host age group (juveniles and adults), and sex. The Mann-Whitney U test was performed to compare the mean intensity between the age groups and sex. RESULTS: Metastrongylus salmi, Metastrongylus apri, and Metastrongylus pudendotectus were identified in 77.6% (45/58) of the necropsied wild boars. Metastrongylus salmi was the most prevalent and abundant species (70.7%, 11.1), followed by M. pudendotectus (18.9%, 4.3) and M. apri (17.2%, 2.2). Metastrongylus pudendotectus showed the highest mean intensity and range (25.2, 1-93), followed by M. salmi (15.7, 1-58) and M. apri (12.6, 3-27). We found a significantly higher prevalence of Metastrongylus spp. and M. salmi in adult wild boars, probably associated with a more prolonged time of exposure to intermediate host species. The phylogenetic analysis revealed that ITS2 region and cox-1 mtDNA are the most suitable genetic markers for Metastrongylus species characterization. Genetic variability between M. apri and M. salmi isolates was verified. CONCLUSIONS: We expand the knowledge about the Metastrongylus community in the non-captive wild boar population from Brazil as well as the importance of this exotic species in the maintenance of Metastrongylus spp. in its areas of occurrence. The novel genetic sequences obtained may help further studies to understand the genetic diversity in other nematode populations from Brazil and other countries.


Assuntos
Metastrongyloidea , Parasitos , Doenças dos Suínos , Suínos , Animais , Brasil/epidemiologia , Filogenia , DNA Ribossômico/genética , DNA Mitocondrial/genética , Sus scrofa , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/parasitologia
4.
Rev Bras Parasitol Vet ; 32(3): e005823, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37851716

RESUMO

Knowledge of taxonomy and biodiversity of parasites is fundamental to better understand ecosystem dynamics. The objective of this study was to describe the helminth fauna of two species of marsupials in five fragments of the Atlantic rainforest in the western region of Paraná State, Brazil. In a total of 4050 trap-nights, the animals were captured using Sherman, Tomahawk, and Pitfall traps, euthanized, necropsied, and their organs inspected for helminths. After identification of the parasites, descriptors of infection, such as prevalence, mean abundance, mean intensity, and range of intensity, were calculated. Collectively, six helminth species were observed in 18 animals. The following five species were observed in Marmosa paraguayana: Viannaia hamata (58.8%), Gracilioxyuris agilisis (52.9%), Travassostrongylus sextus (17.6%), Oncicola luehei (5.9%), and Pritchardia boliviensis (5.9%). Whereas the following two species were observed in Monodelphis dimidiata: Trichohelix tuberculata (100%) and Travassostrongylus sextus (100%). This study represents a new locality record for all helminths described herein, and a new host for four helminth species. This is the first report on the helminth fauna of Monodelphis dimidiata, expanding knowledge about marsupials in the Brazilian Atlantic Forest.


Assuntos
Helmintos , Monodelphis , Animais , Floresta Úmida , Brasil/epidemiologia , Ecossistema
5.
Pathogens ; 12(2)2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36839447

RESUMO

Wild boars (Sus scrofa) are a significant invasive species in Brazil. We evaluated the helminth diversity of 96 wild boars in São Paulo state. Helminth infection descriptors were calculated, the species were identified and their 18S, 28S rDNA and internal transcribed spacer (ITS) regions were amplified for phylogenetic analyses. Ascarops strongylina, Strongyloides ransomi, Globocephalus urosubulatus, Oesophagostomum dentatum, Trichuris suis, Metastrongylus salmi, Metastrongylus pudendotecus, Ascaris suum and Stephanurus dentatus and Macracanthorhynchus hirudinaceus were identified. Globocephalus urosubulatus had the highest prevalence and mean abundance, and most animals had mixed infections with three parasite species. There was no association between parasite intensity and prevalence and host sex and body condition index (p > 0.05). Novel DNA sequences were obtained from G. urosubulatus, A. strongylina, and S. dentatus. This is the first study on the helmint diversity of non-captive wild boars in Brazil, and the first report of the occurrence of M. hirudinaceus, G. urosubulatus and S. dentatus in Brazilian wild boars. Non-captive wild boars of São Paulo State did not act as capture hosts for native helminth species but maintained their typical parasites, common to domestic pigs. They may act as parasite dispersers for low-tech subsistence pig farming and for native Tayassuidae.

6.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 60: e199050, 2023. tab, graf
Artigo em Inglês | VETINDEX, LILACS | ID: biblio-1415384

RESUMO

Gastrointestinal parasites are a significant challenge in sheep farming, and periparturient ewes are one of the most susceptible categories in sheep flocks. This study aimed to verify whether the injection of B vitamins at the acupuncture point governing vessel 14 (GV14) can influence the parasitic load or host-parasite interaction in naturally infected ewes during the peripartum period. Four weeks before parturition, 25 ewes were divided into five treatment groups: (1) Dose Control: 0.2 mL of B complex via IM; (2) Drug Control: 2 mL Complex B via IM; (3) Acupoint Control: 0.2 mL of Water at GV14 point; (4) Control; (5) Test group: 0.2 mL of B complex at the GV14 point. Treatments were performed at weeks -3, -1, 2, 4, and 6 about the parturition time. Fecal samples to estimate the parasitic load by fecal egg counts (FEC) and total blood samples for hemogram examination were performed seven days after the treatment, coinciding with weeks -2, 3, 5, and 7 about the parturition time. The number of peripheral eosinophils was higher in group 3 than in group 1 (p <0.05). Although groups 3 and 5 had lower mean values of FEC and higher hematological values at the time of weaning, these differences were not statistically significant (p> 0.05) according to the F test. However, all animals in groups 3 and 5 maintained low parasitism levels until the end of the experiment. Further studies should be performed with larger sample sizes and minor changes to the experimental protocol to elucidate the role of GV14 acupoint stimulation in the host-parasite relationship.(AU)


A parasitose gastrointestinal é o principal desafio sanitário para a criação de ovinos a pasto, e as ovelhas no periparto são uma das categorias mais susceptíveis a esta infeção no rebanho. O objetivo deste trabalho foi verificar se a injeção de vitaminas do complexo B no acuponto Vaso Governador 14 (VG14) poderia influenciar a carga parasitária ou modular a interação hospedeiro-parasito em ovelhas naturalmente infectadas no período de periparto. Quatro semanas antes da data prevista para o parto, 25 ovelhas foram divididas em cinco grupos de tratamento (1) Controle da dose: 0,2 mL de vitaminas do complexo B via IM; (2) Controle do fármaco: 2mL complexo B via IM; (3) Controle do Ponto: 0,2 mL de água destilada no ponto VG14; (4) Controle sem tratamento; (5) Grupo teste: 0,2 mL de complexo B no pontoVG14. Estes tratamentos foram realizados nas semanas -3; -1; 2; 4 e 6 em relação à data do parto. Amostras de fezes para contagem de ovos por grama de fezes (OPG), assim como amostras de sangue para realizar o hemograma, foram obtidas dos animais sete dias após os tratamentos, coincidindo com as semanas -2, 3, 5 e 7 do parto. O número de eosinófilos periféricos foi superior no grupo 3 se comparado ao grupo 1 (p <0,05). Os animais dos grupos 3 e 5 tiveram menores valores de OPG e maior hematócrito, mas estas diferenças não foram consideradas significativas pelo teste F (p> 0,05). No entanto, todas as ovelhas dos grupos 3 e 5 se mantiveram com níveis baixos de parasitismo durante todo o experimento. Sugere-se que novos estudos sejam realizados com algumas modificações deste protocolo a fim de aprofundar o conhecimento do potencial imunomodulador do acuponto VG14.(AU)


Assuntos
Animais , Feminino , Gravidez , Ovinos/parasitologia , Terapia por Acupuntura/veterinária , Controle de Doenças Transmissíveis/métodos , Complexo Vitamínico B/análise , Período Periparto , Gastroenteropatias
7.
Rev. bras. parasitol. vet ; 32(3): e005823, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1515082

RESUMO

Abstract Knowledge of taxonomy and biodiversity of parasites is fundamental to better understand ecosystem dynamics. The objective of this study was to describe the helminth fauna of two species of marsupials in five fragments of the Atlantic rainforest in the western region of Paraná State, Brazil. In a total of 4050 trap-nights, the animals were captured using Sherman, Tomahawk, and Pitfall traps, euthanized, necropsied, and their organs inspected for helminths. After identification of the parasites, descriptors of infection, such as prevalence, mean abundance, mean intensity, and range of intensity, were calculated. Collectively, six helminth species were observed in 18 animals. The following five species were observed in Marmosa paraguayana: Viannaia hamata (58.8%), Gracilioxyuris agilisis (52.9%), Travassostrongylus sextus (17.6%), Oncicola luehei (5.9%), and Pritchardia boliviensis (5.9%). Whereas the following two species were observed in Monodelphis dimidiata: Trichohelix tuberculata (100%) and Travassostrongylus sextus (100%). This study represents a new locality record for all helminths described herein, and a new host for four helminth species. This is the first report on the helminth fauna of Monodelphis dimidiata, expanding knowledge about marsupials in the Brazilian Atlantic Forest.


Resumo Conhecimentos taxonômicos e da biodiversidade parasitária são fundamentais para uma melhor compreensão da dinâmica do ecossistema. O objetivo do estudo foi descrever a helmintofauna de marsupiais em cinco fragmentos de Mata Atlântica, na região Oeste do Estado do Paraná, Brasil. Em um total de 4.050 armadilhas/noite, os animais foram capturados com as do tipo Sherman, Tomahawk e Pitfall, eutanasiados, necropsiados e seus órgãos inspecionados em busca de helmintos. Após a identificação do parasita, foram calculados os indicadores de infecção (prevalência, abundância média, intensidade média e variação de intensidade). Seis espécies de helmintos foram observadas em dezoito animais; cinco em Marmosa paraguayana: Viannaia hamata (58,8%), Gracilioxyuris agilisis (52,9%), Travassostrongylus sextus (17,6%), Oncicola luehei (5,9%) e Pritchardia boliviensis (5,9%), e duas em Monodelphis dimidiata: Trichohelix tuberculata (100%) e Travassostrongylus sextus (100%). Este estudo representa um novo registro de localidade para todos os helmintos descritos e um novo hospedeiro para quatro deles. Trata-se do primeiro relato da helmintofauna de Monodelphis dimidiata, expandindo-se os conhecimentos sobre marsupiais na mata Atlântica brasileira.

8.
Parasitology ; 148(5): 576-583, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33314998

RESUMO

Pancreatic eurytrematosis (PE) is an under diagnosed and neglected parasitosis in goats and sheep in the Americas. Clinical and pathological features of PE are not well defined in small ruminants worldwide. Natural cases of PE in small ruminants were detected in the Federal District, Brazil. A survey of necropsy records, including epidemiological and clinicopathological data, in goats and sheep was conducted. Most cases of PE occurred during the rainy season in adult females, with an incidence of 12.9% in goats and 0.8% in sheep. Clinical signs varied from asymptomatic infections to anorexia, lethargy, weakness, marked weight loss and death in some goats. Overall, most cases of PE in goats and sheep were incidental necropsy findings with minor pancreatic lesions. Three goats, however, showed severe chronic pancreatitis, dilation of major pancreatic ducts with numerous trematodes present and marked abdominal fat necrosis. Morphological and molecular characterization of flukes detected Eurytrema coelomaticum. Our findings shed light on the prevalence of E. coelomaticum infections in small ruminants in the region and highlight the possibility of severe and lethal cases in goats. PE must be further investigated in small ruminant populations in relevant livestock production regions of the Americas.


Assuntos
Dicrocoeliidae/isolamento & purificação , Doenças das Cabras/epidemiologia , Doenças dos Ovinos/epidemiologia , Infecções por Trematódeos/veterinária , Animais , Brasil/epidemiologia , Feminino , Doenças das Cabras/parasitologia , Cabras , Incidência , Masculino , Prevalência , Ovinos , Doenças dos Ovinos/parasitologia , Carneiro Doméstico , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/parasitologia
9.
Biosci. j. (Online) ; 36(5): 1726-1731, 01-09-2020. tab
Artigo em Inglês | LILACS | ID: biblio-1147921

RESUMO

The objective of this work was to evaluate the proteins, minerals, metabolites, and serum enzymes profile in Quarter Mile equines subjected to training for Team Penning competitions by comparing the results before and after training. The animals were from the Agricultural Exhibition Park of Uberlândia (CAMARU), in Uberlândia in the State of Minas Gerais, Brazil. These animals perform constant periodic training with an average duration of two and a half hours per training. Blood samples from twenty male and female equines were collected at the beginning and end of a 45-day interval and then analyzed. A 5 mL blood sample from each animal was collected before and after training by a jugular external venipuncture, and the samples were placed in a tube­without anticoagulant and containing separator gel­to obtain the serum. The components analyzed were: total proteins, albumin, globulins, albumin to globulin ratio (A:G), total and ionized calcium, phosphorus, calcium to phosphorus ratio (Ca:P), cholesterol, triglycerides, creatinine, urea, alkaline phosphatase, ɤ-glutamyl transferase, lactate dehydrogenase (LDH), aspartate amino transferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK). Statistical differences were observed with higher values after exercise for total calcium, ionized calcium, Ca: P ratio, creatinine and ALT and decreased concentrations were found for globulins, phosphorus, cholesterol and triglycerides. Despite the changes observed in the serum concentrations of some elements after exercises, it was concluded that the animals were in adequate physical condition to perform the proposed physical exercises.


O presente estudo objetivou comparar o perfil de proteínas, metabólitos, minerais e enzimas séricas em equinos Quarto de Milha submetidos a treinamento para provas de Team Penning no Parque de Exposições Agropecuárias de Uberlândia (CAMARU), Uberlândia-MG. Os animais realizavam treinos periódicos e constantes com duração de duas horas e meia em média. Foram analisadas 40 (quarenta) amostras de sangue de 20 (vinte) equinos, machos e fêmeas, coletadas em dois momentos com intervalo de 45 dias. Por meio de venopunção jugular externa, coletou-se 05 (mL) de sangue em tubo sem anticoagulante e contendo gel separador para obtenção de soro, antes e após o treinamento de cada momento. Os constituintes analisados foram proteínas totais, albumina, globulinas, relação albumina globulina (A:G), cálcio total e ionizado, fósforo, relação cálcio fósforo (Ca:P), colesterol, triglicérides, creatinina, ureia, fosfatase alcalina, ɤ-glutamiltransferase (GGT), lactato desidrogenase (LDH), aspartato aminotransferase (AST), alanina aminotransferase (ALT) e creatina quinase (CK). Sendo observado diferenças estatísticas com valores mais elevados após o exercicio para cálcio total, cálcio ionizado, relação Ca:P, creatinina e ALT e concentrações diminuidas para globulinas, fósforo, colesterol e triglicérides. Apesar das alterações observadas nas concentrações séricas de alguns elementos após a prática esportiva, conclui-se que os animais se encontravam em condicionamento físico adequado para realizarem os exercícios físicos propostos.


Assuntos
Proteínas , Cavalos
10.
Rev. patol. trop ; 48(4): 243-248, 2019. ilus
Artigo em Inglês | LILACS | ID: biblio-1099597

RESUMO

The occurrence of Oncicola luehei is reported in a road killed crab-eating raccoon (Procyon cancrivorus) near the municipality of Uberlândia, Minas Gerais State. The animal was collected as part of a study that monitors wildlife road killing in the Triângulo Mineiro region. In necropsy, a single male acanthocephalan was recovered from the large intestine. The parasite was wrinkled, whitish in color, with a total body length of 15.88mm, globular proboscis (0.71 x 0.81mm) armed with 36 spiraled hooks, long lemniscus (7.30 x 0.81 mm) surpassing the anterior testis. The testes were ellipsoid in shape, disposed in tandem, the anterior measuring 1.44 x 0.53mm and the posterior 1.5 x 0.50mm. At the posterior part of the body, eight cement glands arranged in two rows of four, measuring 0.38 x 0.46 mm each. Based on this, the parasite was classified as Oncicola luehei. This study represents a new host and locality records for the parasite.


Assuntos
Guaxinins , Acantocéfalos , Helmintos , Animais Selvagens
11.
Int J Exp Pathol ; 99(5): 226-235, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30456925

RESUMO

Ophidic accidents are among the problems of public health in Brazil. The components from bothropic venom are responsible for many systemic clinical complications resulting from envenomation. The present work aimed to analyse the systemic changes induced in mice after intraperitoneal administration of BmooTX-I, a myotoxic acidic phospholipase A2 isolated from Bothrops moojeni venom. Urinalysis was performed and the following plasma biochemical markers were documented: urea, creatinine and uric acid (renal function); glucose and amylase (pancreatic function); alanine aminotransferase, alkaline phosphatase and gamma-GT (intra- and extrahepatic function); creatine kinase and enzymatic lactate (muscle function). Our results showed that after the intraperitoneal injection of BmooTX-I the urine of these animals showed glycosuria, proteinuria, haematuria, bacteriuria, bilirubinuria, polyuria and nitrite. The plasma biochemical analysis showed alterations in levels of urea, creatinine and uric acid. Amylase concentration was not altered significantly, but the plasma glucose increased significantly compared to controls. The plasma levels of alanine aminotransferase and alkaline phosphatase decreased and increased, respectively, in these same animals. On the other hand, the plasma γGT concentration did not undergo significant modification compared to the control group. The plasma concentration of CK increased, while the enzymatic lactate concentration decreased after the injection of the BmooTX-I. Therefore, in mice BmooTX-I is capable of causing systemic alterations which manifest as renal, muscular, hepatic and pancreatic impairment.


Assuntos
Bothrops , Venenos de Crotalídeos/enzimologia , Fosfolipases A2/toxicidade , Animais , Biomarcadores/sangue , Biomarcadores/urina , Creatina Quinase/sangue , Venenos de Crotalídeos/química , Venenos de Crotalídeos/toxicidade , Injeções Intraperitoneais , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Camundongos , Músculo Esquelético/efeitos dos fármacos , Pâncreas/efeitos dos fármacos , Fosfolipases A2/isolamento & purificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...