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1.
Front Psychol ; 15: 1306122, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38414881

RESUMO

Objective: Childhood sexual abuse (CSA) can have a negative impact on women's psychological, emotional and social functioning. The purpose of this study was to explore the relationship between CSA and depressive symptoms in female college students, as well as the mediating roles of negative core schema and experiential avoidance. Methods: 515 female college students responded to the Sexual Abuse subscale of the Childhood Trauma Questionnaire, the Depression subscale of the Depression Anxiety Stress Scale, the Brief Core Schema Scales, and the Acceptance and Action Questionnaire - II. The structural equation modeling was used for the mediation analysis. Results: There was a significant positive correlation between CSA and depressive symptoms in female college students. The theoretical model was well fitted, χ2/df = 3.422, RMSEA = 0.069, CFI = 0.929, TLI = 0.919. The negative core schema played a mediating role between CSA and depressive symptoms. Experiential avoidance played a mediating role between CSA and depressive symptoms. The negative core schema and experiential avoidance played a serial mediating role between CSA and depressive symptoms. Conclusion: These results deepen our understanding of the relationship between CSA and depressive symptoms in female college students, and provide theoretical guidance for the prevention of depression in female college students. Attention should be paid to female college students who have experienced CSA, to eliminate the adverse influence of negative core schema on these students. Meanwhile, we should teach female college students to accept themselves as they are, and thereby reduce their use of experiential avoidance strategies.

2.
BMC Psychiatry ; 24(1): 147, 2024 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-38383358

RESUMO

BACKGROUND: Postpartum depression (PPD) is a major public health problem worldwide. Previous studies have shown that postpartum negative life events and neuroticism are both important risk factors for PPD. However, few studies have considered the role of protective factors in the influence of postpartum negative life events and neuroticism on PPD. Based on the diathesis-stress model and Acceptance and Commitment Therapy (ACT), a moderated mediating model was established to examine the mediating role of neuroticism between postpartum negative life events and PPD, as well as the moderating role of psychological flexibility in this mediating effect. METHODS: A sample of 776 parturients from three different Grade A hospitals in China were assessed using the Edinburgh Postpartum Depression Scale, the Postpartum Negative Life Events Scale, the Neuroticism Subscale of the Big Five Personality Scale, and the Acceptance and Action Questionnaire- II. RESULTS: PPD, postpartum negative life events, neuroticism, and experiential avoidance were significantly positively correlated with one another. Neuroticism partially mediated the relationship between postpartum negative life events and PPD. In this mediation model, the direct path and the second half of the mediation path were moderated by psychological flexibility. Specifically, the links between postpartum negative life events and PPD, as well as between neuroticism and PPD, were stronger when psychological flexibility was low, but weaker when psychological flexibility was high. CONCLUSIONS: The results show that psychological flexibility plays an important role in buffering the negative effects of postpartum negative life events and neuroticism on PPD. These findings provide implications for the prevention and intervention of PPD using an ACT approach.


Assuntos
Terapia de Aceitação e Compromisso , Depressão Pós-Parto , Feminino , Humanos , Depressão Pós-Parto/psicologia , Neuroticismo , Período Pós-Parto/psicologia , Personalidade , Fatores de Risco
3.
Sci Total Environ ; 918: 170403, 2024 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-38307282

RESUMO

Northwest China has been experiencing severe land degradation for a long time due to various natural and social elements. Evaluating and analyzing the process of occurrence and driving mechanism of land degradation sensitivity in this area is crucial for enhancing the local ecological environment. In this study, 18 social and environmental elements were used to construct a land degradation sensitivity index (LDSI) evaluation system in the area from vegetation, climate, management, soil, and geomorphology five factors. The spatio-temporal characteristics of LDSI in Northwest China from 2000 to 2020 were evaluated on the basis of analyzing the developmental changes of each factor. Correlation analysis and multiscale geographical weighting regression (MGWR) were used to reveal the driving mechanism of land degradation sensitivity. The results indicated a high level of land degradation sensitivity in Northwest China, with >66 % of the area (190.96 × 104 km2) in the critical sensitive class from 2000 to 2020. But the land degradation sensitivity decreased in 18.52 % of the area (53.58 × 104 km2) from 2000 to 2020, the overall trend was weakening. The spatial distribution mainly showed stronger sensitivity in the northwest and weaker sensitivity in the southeast. By exploring the driving mechanism of land degradation sensitivity, it was found that vegetation and climate showed a strong correlation, with a correlation coefficient >0.8. Drought resistance played a strong role in the dynamic process of land degradation. The basic dynamic elements showed some spatial variability in land degradation in different regions. This study is of significance for land degradation prevention and sustainable development in Northwest China.

4.
Medicine (Baltimore) ; 102(46): e36047, 2023 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-37986401

RESUMO

BACKGROUND: Severe community-acquired pneumonia (sCAP) is characterized by severe symptoms and a poor prognosis, especially with the recent global impact of novel coronavirus in recent years. The use of glucocorticoids in sCAP is currently a subject of debate. To evaluate the clinical efficacy and safety of glucocorticoids and provide guidance for their rational use in clinical practice, we conducted this study. METHODS: We searched PubMed, Web of Science, and China National Knowledge Infrastructure using the following search terms: "pneumonia", "pneumonias", "Pulmonary Inflammation", "Pulmonary Inflammations", "Lung Inflammation", and "Lung Inflammations". The primary outcomes included mortality and the length of hospital stay. The secondary outcomes included the duration of mechanical ventilation, duration of vasoactive drug use, gastrointestinal bleeding, and multiple infections. The Cochrane Collaboration was used to assess the risk of bias of the included studies. Stata/MP14 was used for meta-analysis. RESULTS: These studies contained information on 1252 patients who received glucocorticoids and 1280 patients who did not. Meta-analysis showed that there was no difference in terms of mortality [risk ratio (RR) = 0.93, 95% confidence interval (CI): 0.81-1.07, P  > .05], gastrointestinal bleeding (RR = 1.38, 95% CI: 0.83-2.30, P  <  .05), multiple infections (RR = 1.17, 95% CI: 0.90-1.53, P  > .05) and length of hospital stay (mean difference [MD] = -0.87, 95% CI: -2.35 to 0.61, P  > .05) between the hormonal and nonhormonal groups. However, there was a significant difference in the duration of mechanical ventilation (MD = -1.54; 95% CI, -1.89 to -1.12, P  <  .05) and the duration of use of vasoactive drugs (MD = -14.09, 95% CI: -15.72 to -12.46, P < .05). CONCLUSION: Glucocorticoids reduced the duration of mechanical ventilation duration and vasoactive drug use in sCAP patients without increasing the risk of adverse events including hyperglycemia and multiple infections. However, there was no significant difference in mortality or length of hospital stay in sCAP patients between glucocorticoid and non-glucocorticoid groups. Glucocorticoids could be recommended for patients with sCAP with respiratory failure or hemodynamic instability.


Assuntos
Infecções Comunitárias Adquiridas , Pneumonia , Humanos , Glucocorticoides/uso terapêutico , Ensaios Clínicos Controlados Aleatórios como Assunto , Pneumonia/tratamento farmacológico , Infecções Comunitárias Adquiridas/tratamento farmacológico , Hemorragia Gastrointestinal/tratamento farmacológico , Inflamação
5.
Front Public Health ; 11: 1137921, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37457254

RESUMO

Objective: To explore the mediating role of depression in the relationship between family functioning and suicidal ideation (SI) in college students, and to explore whether acceptance (It is one of the core components of psychological flexibility) plays a moderating role in this mediating model. Methods: In a cross-sectional study, questionnaires were distributed to college students during November and December 2022. The sample of Chinese college students (n = 592, 43.07% male, 56.93% female, mean age 19.40 years, SD = 1.24 years) completed the Family Adaptability and Cohesion Evaluation Scale (FACES III), the Center for Epidemiological Depression Scale (CES-D), the Positive and Negative Suicide Ideation Inventory (PANSI), and the Acceptance and Action Questionnaire-Second Edition (AAQ II). SPSS 25.0 for Windows and PROCESS 2.15 macros were used for data analysis. Results: There was a significant negative correlation between family functioning and SI, and depression played a mediating role in this relationship. Acceptance moderated the indirect effects of depression and SI in college students. In college students with a lower acceptance level (i.e., higher experiential avoidance level), depression had more influence on SI, while the influence of depression on SI was less in college students with a higher acceptance level (i.e., lower experiential avoidance level). Family functioning indirectly influenced SI through the moderation of acceptance. Conclusion: Mental health educators in colleges and universities should pay more attention to identifying and relieving depression in college students, thereby dealing with suicide risk more effectively. At the same time, college students should be discouraged from excessive use of experiential avoidance strategies, and instead taught to master effective emotional regulation strategies such as mindfulness, distress tolerance, and radical acceptance skills to improve their acceptance level and alleviate the influence of depression on SI.


Assuntos
Depressão , Ideação Suicida , Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Depressão/psicologia , Estudos Transversais , Inquéritos e Questionários , Estudantes/psicologia
6.
Biology (Basel) ; 12(4)2023 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-37106794

RESUMO

Plant and algal LDs are gaining popularity as a promising non-chemical technology for the production of lipids and oils. In general, these organelles are composed of a neutral lipid core surrounded by a phospholipid monolayer and various surface-associated proteins. Many studies have shown that LDs are involved in numerous biological processes such as lipid trafficking and signaling, membrane remodeling, and intercellular organelle communications. To fully exploit the potential of LDs for scientific research and commercial applications, it is important to develop suitable extraction processes that preserve their properties and functions. However, research on LD extraction strategies is limited. This review first describes recent progress in understanding the characteristics of LDs, and then systematically introduces LD extraction strategies. Finally, the potential functions and applications of LDs in various fields are discussed. Overall, this review provides valuable insights into the properties and functions of LDs, as well as potential approaches for their extraction and utilization. It is hoped that these findings will inspire further research and innovation in the field of LD-based technology.

7.
Oxid Med Cell Longev ; 2023: 1493684, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36778209

RESUMO

Objective: Patients with severe asthma respond poorly to corticosteroids, and their care accounts for more than 60% of the total costs attributed to asthma. Neutrophils form neutrophil extracellular traps (NETs), which play a crucial role in severe asthma. Statins have shown anti-inflammatory effects by reducing NETosis. In this study, we investigate if simvastatin can attenuate severe asthma by reducing NETosis and the underlying mechanism. Methods: Mice were concomitantly sensitized with ovalbumin (OVA), house dust mite (HDM), and lipopolysaccharide (LPS) during sensitization to establish a mouse model of severe asthma with neutrophil predominant inflammation (OVA+LPS mice) and treated with or without simvastatin. In inflammatory response, proportions of Th2, Th17, and Treg cells in lung tissue were detected by flow cytometry, and the levels of cytokines, dsDNA, and MPO-DNA in bronchoalveolar lavage fluid (BALF) were analyzed by ELISA. Citrullinated histone H3 (CitH3) and peptidyl arginine deiminase 4 (PAD4) in lung tissue were determined by Western blot and immunofluorescence imaging. PAD4 mRNA was determined by quantitative PCR (qPCR). HL-60 cells were differentiated into neutrophil-like cells by 1.25% DMSO. The neutrophil-like cells were treated with or without LPS, and simvastatin was then stimulated with PMA. CitH3 and PAD4 expressions were determined. Results: Sensitization with OVA, HDM, and LPS resulted in neutrophilic inflammation and the formation of NETs in the lungs. Simvastatin treatment reduced the inflammation score, cytokine levels, total cells, and neutrophil counts in the BALF and reduced proportions of Th2 and Th17 but increased Treg cells in lungs of OVA+LPS mice. Simvastatin-treated OVA+LPS mice show reduced NET formation in BALF and lung tissue compared to control mice. Adoptive transfer of neutrophils was sufficient to restore NETosis and neutrophilic inflammation in simvastatin-treated OVA+LPS mice. Simvastatin reduced PAD4 mRNA and protein expression in lung tissues and neutrophils isolated from lungs of OVA+LPS mice and consequent NET formation. In vitro, simvastatin reduced LPS-induced PAD4 upregulation and NETosis in HL-60-differentiated neutrophil-like cells. Furthermore, PAD4-overexpressed lentiviral transduction was sufficient to restore PAD4 protein expression and NETosis in simvastatin-treated HL-60-differentiated neutrophil-like cells. Conclusions: Simvastatin reduces Th17-mediated neutrophilic inflammation and airway hyperreactivity by reducing PAD4 expression and inhibiting NETosis in a mouse model of severe asthma. Severe asthmatic patients with high levels of circulating NETs or sputum NETs may show improved responses to statin treatment.


Assuntos
Asma , Sinvastatina , Animais , Camundongos , Asma/tratamento farmacológico , Asma/metabolismo , Líquido da Lavagem Broncoalveolar , Citocinas/metabolismo , Modelos Animais de Doenças , DNA/metabolismo , Histonas/metabolismo , Inflamação/metabolismo , Lipopolissacarídeos/farmacologia , Pulmão/metabolismo , Neutrófilos/metabolismo , Ovalbumina , RNA Mensageiro/metabolismo , Sinvastatina/farmacologia , Sinvastatina/uso terapêutico , Armadilhas Extracelulares
8.
Curr Pharm Biotechnol ; 24(2): 310-316, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-35570553

RESUMO

BACKGROUND: Human brain tumor glioblastoma (GBM) is the most hostile malignancy, currently lacking a successful cure and good prognosis. OBJECTIVE: To examine the anticancer effects of syringic acid (SA) on human cancer GBM cells. METHODOLOGY: The different doses of SA were added to GBM cells to study its effect on viability, invasion, relocation, apoptosis, and mRNA and protein levels. Hence, we explored the antiproliferative, anti-invasive, and apoptotic activity of SA on GBM human U-251 cells. RESULTS: MTT assay and live/dead assay revealed the anti-proliferative activity of SA on U-251 glioma cells. Apoptotic activity of SA was shown by DAPI staining, caspase-3, Bax, and Bcl-2 mRNA expressions. The cell cycle regulation was also confirmed by reducing the mRNA expression of cyclinD1, CDK4, and CDK6. Treatment of SA with U-251 cells suppressed MMPs expressions and enhanced TIMPs protein levels. CONCLUSION: Our findings put forward that SA could prevent GBM cells' invasion and relocation. SA is an ideal neuroprotective agent for controlling brain malignancy.


Assuntos
Neoplasias Encefálicas , Glioblastoma , Humanos , Glioblastoma/tratamento farmacológico , Glioblastoma/genética , Proliferação de Células , Linhagem Celular Tumoral , Apoptose , Neoplasias Encefálicas/tratamento farmacológico , Neoplasias Encefálicas/genética , RNA Mensageiro
9.
Sci Rep ; 12(1): 16034, 2022 09 26.
Artigo em Inglês | MEDLINE | ID: mdl-36163366

RESUMO

Continuous rigid-frame bridges are widely used, but the large deflection in the mid-span during operation has always been their disease. This problem is generally solved by setting the finished bridge pre-camber. There are many calculation methods for pre-camber, and the effects are different. In this paper, based on a large number of design parameters of continuous rigid-frame bridges obtained from the investigation, 18 finite element analysis models of different span combinations were established, and 30 sets of valid data were obtained under the action of multi-factor. The results show that the shrinkage and creep of concrete is the most important factor for the mid-span deflection of continuous rigid frame bridges, and the deflection amount has an obvious functional relationship with the span. The effect of prestress loss on mid-span deflection is second, and stiffness reduction has little effect on mid-span long-term deflection. In this paper, the least-squares method is used to perform polynomial fitting, and the fitting formula for the mid-span finished bridge pre-camber is finally obtained. The applicability of the calculation formula is proved by comparing it with the specification solution, the empirical solution, and the measured value.


Assuntos
Algoritmos , Análise de Elementos Finitos
10.
Sci Rep ; 12(1): 13372, 2022 08 04.
Artigo em Inglês | MEDLINE | ID: mdl-35927453

RESUMO

The ecological environment of the hilly and gully area of the Loess Plateau in northern Shaanxi is fragile and the soil fertility is low. As a result, the yield and quality of Pear-jujube which constitute one of the dominant economic forests in this region, have been severely restricted. At present, the scientific application of fertilizer is important for comprehensively improving the quality of fruit trees, and for devising the optimal management of fruit trees. In particular, the application of organic fertilizers plays an important role in improving soil and improving fruit quality. In this experiment, a field study was conducted to understand the effects of different organic fertilizer applications on physiological growth, photosynthetic characteristics, reproductive growth and nutritional quality of Pear-jujube in the Loess Plateau. The results showed that organic fertilizer significantly promoted the physiological growth of Pear-jujube. The Pear-jujube bearing branch and leaf area under the soybean cake fertilizer (SC) treatment were 20.17 cm and 1246 mm2/leaf, respectively, which are increased by 34% and 44.46% compared with the no fertilizer treatment, which was a control check (CK). The total chlorophyll content of fertilization treatment was significantly higher than that of CK (P < 0.05). The maximum of chlorophyll content was 10.90 mg/dm2 under the biogas fertilizer (BM). The content of LAI was in the order BM > conventional fertilizer (CF) > sheep manure (SM) > SC > CK. The changing trend of gap fraction was opposite to that of LAI, and the density of light was consistent with that of LAI. The density of light BM was the largest, reached 38.06 mol/(m2 d), which was 15.13% higher than that of CK. Organic fertilizer significantly improved the net photosynthetic rate (Pn) and water use efficiency (WUEp) of Pear-jujube. The WUEp of SC was up to 3.30%. Organic fertilizer significantly promoted the reproductive growth and improved the nutritional quality of Pear-jujube. The yield under the SC was 19,177 kg/hm2, increased by 138.5% compared with that of CK. The fruit water content (FWC), total soluble solids (TSS), solid-acid ratio (TSS/TA), Vc and total flavonoids content improved, and the maxima of FWC, TSS, TSS/TA, Vc and total flavonoids content under the SC treatment were 86.30%, 18.48%, 40.17, 46.18 mg/kg and 14.35 mg/kg, respectively, which were significantly different from those of CK (P < 0.05). Organic fertilization effectively promotes the growth, development, yield and fruit quality of Pear-jujube in the Loess Plateau and the effect of the soybean cake fertilizer is the most significant.


Assuntos
Pyrus , Ziziphus , Agricultura/métodos , Animais , China , Clorofila , Fertilizantes , Flavonoides , Florestas , Frutas , Nitrogênio , Ovinos , Solo , Água
11.
Front Psychol ; 13: 962147, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36017432

RESUMO

In consideration of family system theory, the vulnerability model of depression, and the stress buffering model of social support, the current study examined the effect of family functioning on adolescent depression, the mediating effect of self-esteem, and the moderating effect of peer relationships. A sample of Chinese adolescents (n = 562, 47.15% male, 52.85% female, mean age 14.33 years, SD = 1.81 years) completed questionnaires regarding family functioning, depression, self-esteem, and peer relationships. The results showed that: (1) family functioning had a significant negative predictive effect on adolescent depression; (2) self-esteem plays a mediating role between family functioning and adolescent depression; and (3) peer relationships have a moderating effect on the relationship between self-esteem and adolescent depression, supporting the moderated mediation model. These results reveal the influence mechanism of family functioning on adolescent depression and have implications for adolescent depression intervention.

12.
Cell Mol Biol (Noisy-le-grand) ; 67(6): 149-154, 2022 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-35818202

RESUMO

The occurrence of glioma is gradually promoted by various factors, and it has gone through multiple stages of development, involving abnormal expression of multiple genes. One of the important reasons for the development of gliomas is the interaction of genetic factors and the environment. Non-coding transcripts can also form this high-level structure, and the formation of binding sites for interactions between lncRNA and proteins, DNA, and other RNA molecules may be related to their structural diversity. Due to the importance of glioma-related research and the potential effectiveness of lncRNA, this paper focuses on the mechanism of long-chain non-coding RNA targeting the Mir signal axis to regulate apoptosis, invasion and migration of glioma U251 cells. In this paper, human glioma cell line U251 was used as experimental material for simulation analysis. The results showed that after miR simulation, the pass rate of U251 stem cells through the filter was 17.3%, which was significantly less than 85.4% of group C; compared with 77.6% of the negative control group, the cell penetration rate of the miR inhibitor group was significantly improved. 92.5%. The miR expression level can affect the invasion ability of U251 stem cells, and can negatively regulate the expression of fzd4 to inhibit the invasion and metastasis of glioma U251 cells.


Assuntos
Glioma , MicroRNAs , RNA Longo não Codificante , Apoptose/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Receptores Frizzled/genética , Receptores Frizzled/metabolismo , Regulação Neoplásica da Expressão Gênica , Glioma/patologia , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Invasividade Neoplásica/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo
13.
Phys Chem Chem Phys ; 24(21): 13066-13073, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35583864

RESUMO

Criegee intermediates are important oxidants produced in the ozonolysis of alkenes in the atmosphere. Quantitative kinetics of the reactions of Criegee intermediates are required for atmospheric modeling. However, the experimental studies do not cover the full relevant range of temperature and pressure. Here we report the quantitative kinetics of CH2OO + CH3C(O)CH3 by using our recently developed dual strategy that combines coupled cluster theory with high excitation levels for conventional transition state theory and well validated levels of density functional theory for direct dynamics calculations using canonical variational transition theory including tunneling. We find that the W3X-L//DF-CCSD(T)-F12b/jun-cc-pVDZ electronic structure method can be used to obtain quantitative kinetics of the CH2OO + CH3C(O)CH3 reaction. Whereas previous investigations considered a one-step mechanistic pathway, we find that the CH2OO + CH3C(O)CH3 reaction occurs in a stepwise manner. This has implications for the modeling of Criegee-intermediate reactions with other ketones and with aldehydes. In the kinetics calculations, we show that recrossing effects of the conventional transition state are negligible for determining the rate constant of CH2OO + CH3C(O)CH3. The present findings reveal that the rate ratio between CH2OO + CH3C(O)CH3 and OH + CH3C(O)CH3 has a significant negative dependence on temperature such that the CH2OO + CH3C(O)CH3 reaction can contribute as a significant sink for atmospheric CH3C(O)CH3 at low temperature. The present findings should have broad implications in understanding the reactions of Criegee intermediates with carbonyl compounds and ketones in the atmosphere.

14.
Front Psychol ; 13: 792108, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35465482

RESUMO

Objective: To explore the relationship between adolescents' social mobility belief and their learning engagement, as well as the mediating effect of achievement goal orientation and the moderating effect of psychological capital. Method: A sample of 895 adolescents from Hunan, Jiangxi, Hainan, Henan, and Guangdong provinces were assessed using the social mobility belief questionnaire, the achievement goal orientation questionnaire, the adolescents' psychological capital questionnaire, and the adolescents' learning engagement questionnaire. Results: First, adolescents' social mobility belief was positively related to their learning engagement (r = 0.481, p < 0.01); Second, the two achievement goal orientations-mastery goal orientations and performance goal orientations-had mediating effects between social mobility belief and learning engagement (r = 0.603, p < 0.01; r = 0.367, p < 0.01); Third, the relationship between achievement goal orientation and learning engagement was regulated by psychological capital. Adolescents with high psychological capital had higher learning engagement (r = 0.684, p < 0.01). Conclusion: Adolescents' social mobility belief can directly affect their learning engagement, and it can also indirectly affect their learning engagement through achievement goal orientation moderated by their level of psychological capital. Our findings highlighted the importance of providing adolescents with guidance in setting up positive social values and definitions of success while stimulating their psychological capital as a part of the teaching process.

15.
Front Genet ; 13: 843711, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35401698

RESUMO

The overall survival of patients with lower grade glioma (LGG) that might develop into high-grade malignant glioma shows marked heterogeneity. The currently used clinical evaluation index is not sufficient to predict precise prognostic outcomes accurately. To optimize survival risk stratification and the personalized management of patients with LGG, there is an urgent need to develop an accurate risk prediction model. The TCGA-LGG dataset, downloaded from The Cancer Genome Atlas (TCGA) portal, was used as a training cohort, and the Chinese Glioma Genome Atlas (CGGA) dataset and Rembrandt dataset were used as validation cohorts. The levels of various cancer hallmarks were quantified, which identified glycolysis as the primary overall survival-related risk factor in LGGs. Furthermore, using various bioinformatic and statistical methods, we developed a strong glycolysis-related gene signature to predict prognosis. Gene set enrichment analysis showed that in our model, high-risk glioma correlated with the chemoradiotherapy resistance and poor survival. Moreover, based on established risk model and other clinical features, a decision tree and a nomogram were built, which could serve as useful tools in the diagnosis and treatment of LGGs. This study indicates that the glycolysis-related gene signature could distinguish high-risk and low-risk patients precisely, and thus can be used as an independent clinical feature.

16.
Metab Brain Dis ; 37(4): 1133-1143, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35254598

RESUMO

Glioblastoma (GBM), a predominant central nervous system (CNS) malignancy, is correlated with high mortality and severe morbidity. Mammalian methyltransferase-like 7B (METTL7B) as a methyltransferase has been identified to participate in cancer progression. However, its function in GBM is elusive. Accordingly, we aimed to explore the effect of METTL7B on GBM. The expression of METTL7B and EGR2 in GBM patients and GBM cells were detected by qPCR, western blots and immunohistochemical staining. Cell viability was assessed by CCK-8 assays. Cell proliferation was determined by EdU, colony formation, and tumor sphere formation assays. METTL7B shRNA was injected into the Balb/c nude mice. The size and weight of isolated tumor was measured. And the expression levels of Ki67, METTL7B and EGR1 were examined by immunohistochemical staining. METTL7B was significantly elevated, while EGR1 was downregulated in clinical GBM tissues. METTL7B upregulation was associated with the low overall survival of GBM patients. Moreover, METTL7B depletion remarkably attenuated GBM cell proliferation. Mechanistically, METTL7B overexpression inhibited EGR1 expression in GBM cells. EGR1 knockdown rescued the inhibitory effect of METTL7B depletion on GBM cell proliferation. Meanwhile, METTL7B depletion arrested more GBM cells at the G0/G1, but fewer cells at the S phase, which EGR1 knockdown reversed these effects. Furthermore, tumorigenicity analysis revealed that METTL7B promotes tumor growth of GBM cells in vivo. METTL7B contributes to the malignant progression of GBM by inhibiting EGR1 expression. METTL7B and EGR1 may be utilized as the treatment targets for GBM therapy.


Assuntos
Proteínas de Transporte/metabolismo , Glioblastoma , Animais , Linhagem Celular Tumoral , Proliferação de Células , Proteína 1 de Resposta de Crescimento Precoce/genética , Glioblastoma/metabolismo , Humanos , Mamíferos , Metiltransferases/genética , Camundongos , Camundongos Nus
17.
J Orthop Surg Res ; 16(1): 687, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34809649

RESUMO

OBJECTIVE: To compare the effects between computer-assisted and traditional cannulated screw internal fixation on treating femoral neck fracture. METHODS: The search was conducted in Embase, Pubmed, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI) and Wanfang Database from the beginning to August 2020. RevMan5.4 software, which was provided by the International Cochrane Group, was used for the meta-analysis comparing the differences in operation time, intraoperative bleeding volume, fluoroscopy frequency, fracture healing time, total drilling times, Harris score, fracture healing rate, and femoral head necrosis rate between computer-assisted and traditional methods groups. RESULTS: A total of 1028 patients were included in 16 studies. Primary outcome indicators: Compared with the traditional method group, the computer-assisted group had less operative time (2RCTs, P < 0.00001; 8 non-RCTs, P = 0.009; Overall, P < 0.00001), intraoperative bleeding (1 RCTs, P < 0.00001; 9non-RCTs, P < 0.00001; Overall, P < 0.00001), femoral head necrosis rate (1 RCT, P = 0.11;7 non-RCTs, P = 0.09; Overall, P = 0.02) and higher Harris scores (1 RCT, P < 0.0001; 9 non-RCTs, P = 0.0002; Overall, P < 0.0001), and there were no significant differences in fracture healing rate between the two groups (5 non-RCTs, P = 0.17). Secondary outcomes indicators: The computer-assisted group had a lower frequency of intraoperative fluoroscopy and total number of drills compared with the traditional method group, while there was no significant difference in fracture healing time. CONCLUSION: Compared with the traditional hollow screw internal fixation on the treatment of femoral neck fracture, computer-assisted percutaneous cannulated screw fixation can shorten the operation time and improve the operation efficiency and reduce the X-ray injury of medical staff and help patients obtain a better prognosis. Therefore, computer-assisted percutaneous cannulated screw fixation is a better choice for the treatment of femoral neck fracture. Study registration PROSPERO registration number CRD42020214493.


Assuntos
Fraturas do Colo Femoral , Parafusos Ósseos , Computadores , Fraturas do Colo Femoral/diagnóstico por imagem , Fraturas do Colo Femoral/cirurgia , Necrose da Cabeça do Fêmur , Fixação Interna de Fraturas/efeitos adversos , Humanos , Resultado do Tratamento
18.
Front Psychol ; 12: 738170, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721210

RESUMO

Based on interpersonal acceptance-rejection theory, family systems theory, and psychological inflexibility theory, this study aimed to examine the mediating roles of a protective factor (self-esteem) and a risk factor (psychological inflexibility) on the influence of parenting style on adolescent mental health. A sample of Chinese adolescents (n = 916, 46% male, mean age = 14.44 years, SD = 1.84 years) completed the Short Egna Minnen Barndoms Uppfostran, the Rosenberg Self-esteem Scale, the Avoidance and Fusion Questionnaire for Youth, the Satisfaction with Life Scale, and the Depression Subscale of the Youth Self-Report. Results show that the self-esteem and psychological inflexibility play a chain mediating role in the relationship between parenting style and adolescent mental health. Specifically, parental emotional warmth had a positive effect on adolescent mental health through the chain mediating effects of self-esteem and psychological inflexibility. Parental rejection and parental over-protection had negative effects on adolescent mental health by lowering self-esteem but increasing psychological inflexibility. These results provide further guidance in the prevention of and intervention in adolescent mental health problems.

19.
PLoS One ; 16(6): e0253209, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34143822

RESUMO

To realize the accurate extraction of surface water in complex environment, this study takes Sri Lanka as the study area owing to the complex geography and various types of water bodies. Based on Google Earth engine and Sentinel-2 images, an automatic water extraction model in complex environment(AWECE) was developed. The accuracy of water extraction by AWECE, NDWI, MNDWI and the revised version of multi-spectral water index (MuWI-R) models was evaluated from visual interpretation and quantitative analysis. The results show that the AWECE model could significantly improve the accuracy of water extraction in complex environment, with an overall accuracy of 97.16%, and an extremely low omission error (0.74%) and commission error (2.35%). The AEWCE model could effectively avoid the influence of cloud shadow, mountain shadow and paddy soil on water extraction accuracy. The model can be widely applied in cloudy, mountainous and other areas with complex environments, which has important practical significance for water resources investigation, monitoring and protection.


Assuntos
Monitoramento Ambiental/métodos , Imagens de Satélites , Recursos Hídricos , Água , Sri Lanka
20.
Front Psychol ; 12: 773421, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-35115984

RESUMO

This study examined the effect of self-support on loneliness, the mediation effect of school belonging, and the moderation effect of self-esteem using a sample comprising 1,126 Chinese mainland primary school students, 621 are boys and 505 are girls, and their mean age was 10.51 years (SD = 1.63, range 8-13). Participants completed questionnaires regarding self-support, loneliness, school belonging and self-esteem. In the model hypothesis, self-support is an independent variable, loneliness is an outcome variable, school belonging is a mediating variable, and self-esteem is a regulatory variable. After controlling the demographic variables, the data were analyzed, and the results showed that: (1) self-support had a significantly negative predictive effect on loneliness; (2) the relation between self-support and loneliness was mediated by school belonging; and (3) the relation between school belonging and loneliness was moderated by self-esteem, supporting the moderated mediation model. Moderated mediation analysis further indicated that the mediated path make loneliness weaker for pupils with higher levels of self-esteem. These results revealed the formation mechanism of loneliness in primary school students and have certain enlightenment significance for the intervention of loneliness in primary school students. These results revealed the formation mechanism of loneliness among primary school students and have significant implications for interventions against loneliness in the primary school context.

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