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1.
Sci Adv ; 6(3): eaay4508, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-32010770

RESUMO

Monolithic strong magnetic induction at the mtesla to tesla level provides essential functionalities to physical, chemical, and medical systems. Current design options are constrained by existing capabilities in three-dimensional (3D) structure construction, current handling, and magnetic material integration. We report here geometric transformation of large-area and relatively thick (~100 to 250 nm) 2D nanomembranes into multiturn 3D air-core microtubes by a vapor-phase self-rolled-up membrane (S-RuM) nanotechnology, combined with postrolling integration of ferrofluid magnetic materials by capillary force. Hundreds of S-RuM power inductors on sapphire are designed and tested, with maximum operating frequency exceeding 500 MHz. An inductance of 1.24 µH at 10 kHz has been achieved for a single microtube inductor, with corresponding areal and volumetric inductance densities of 3 µH/mm2 and 23 µH/mm3, respectively. The simulated intensity of the magnetic induction reaches tens of mtesla in fabricated devices at 10 MHz.

2.
Chem Commun (Camb) ; 56(3): 462-465, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31825446

RESUMO

In this study, a multi-step phase transition hybrid composed of (Pr-dabco)2Ag4I6 clusters (Pr-dabco+ = 1-propyl-1,4-diazabicyclo[2.2.2]octan-1-ium) has been prepared and characterized by microanalysis, IR and UV-vis spectroscopy, TG and DSC techniques, etc. This hybrid is thermally stable up to ∼486 K with five phases in the temperature region below 486 K. The phase transition shows symmetry breaking (SB) character between phases II (space group P21/c) and III (space group Pa3[combining macron]), while inverse symmetry breaking (ISB) between phases II and I (space group Pbca), and it is rather exceptional for matter to exhibit simultaneously SB and ISB nature in two successive phase transitions. Most importantly, each phase transition is associated with a dielectric anomaly, and phase V appears to be a plastic crystal with extra high ac conductivity (>10-2 S cm-1). Our work opens up new avenues to find a multi-phase transition material in silver halide hybrids.

3.
BMC Vet Res ; 15(1): 438, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801544

RESUMO

BACKGROUND: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. RESULTS: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80, 15.61, 9.27 and 2.44%, respectively. CONCLUSIONS: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

4.
Nanotechnology ; 30(36): 364001, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31121568

RESUMO

Monolithic capacitors operating at radio frequencies (RF) serve as critical components in integrated circuits for wireless communication. Design and fabrication innovations for high capacitance density RF capacitors are highly desired for the miniaturization of RFIC chips. However, practical and simple solutions are limited by existing capabilities in three-dimensional (3D) structure construction and the effective configuration of electrodes. We report a unique route to achieve unprecedentedly high capacitance density at a high operating frequency through a capacitor configuration of 3D coil interdigital electrodes using planar semiconductor processing compatible materials and fabrication methods. A systematic mechanical-electrical design principle is demonstrated, and fabricated devices show a maximum 21.5 pF capacitance, which is 17.2× larger after rolling up. The corresponding capacitance density is as large as 371 pF mm-2, with resonant frequency of 1.5 GHz. The performance could be improved significantly by simply rolling up more turns with minimal change to the area footprint.

5.
J Cell Biochem ; 118(9): 2581-2586, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28106294

RESUMO

The biological consequences of cellular senescence and immortalization in aging and cancer are in conflict. Organisms have developed common cellular signaling pathways and surveillance mechanisms to control the processing of aging against tumorigenesis. The imbalance of any signals involved in this process may result in either premature aging or tumorigenesis and reduce the life span of the organism. In contrast, the balance between aging and tumorigenesis at a higher level (homeostatic-balance) may benefit the organism with tumor-free longevity. The focus of this perspective is to review the literature on the balance between "Yin" and "Yang" in traditional Chinese medicine. Modern cellular and molecular techniques now permit a more robust system to screen herbs in traditional Chinese medicine for their activity in balancing aging and tumorigenesis. The understanding of the crosstalk between aging and tumorigenesis and new perspectives on the application of Chinese medicine might shed light on anti-aging and tumor-free strategies. J. Cell. Biochem. 118: 2581-2586, 2017. © 2017 Wiley Periodicals, Inc.


Assuntos
Envelhecimento , Medicina Tradicional Chinesa , Biologia Molecular , Neoplasias , Plantas Medicinais , Envelhecimento/genética , Envelhecimento/metabolismo , Humanos , Neoplasias/genética , Neoplasias/metabolismo , Neoplasias/terapia
6.
Exp Ther Med ; 12(4): 2747-2752, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27698780

RESUMO

The incidence of oral squamous cell carcinoma (OSCC) is continuously increasing while its survival rate has not notably improved. There is a pressing need for improved understanding of the genetic regulation of OSCC tumorigenesis and progression. In this study, the function of miR-448 in the regulation of OSCC growth and its putative target were thoroughly analyzed in vitro. The expression of miR-448 was detected in human OSCC specimens and OSCC cell lines (Cal-27 and Scc-9) by reverse transcription-quantitative polymerase chain reaction. The function of miR-448 was investigated in Cal-27 cells transfected with miR-448 inhibitor, and its putative target determined using a luciferase reporter assay. MTT and wound healing assays and flow cytometry were used to evaluate the effects of miR-448 on OSCC proliferation, metastasis and apoptosis. The level of miR-448 was significantly elevated in human OSCC tissues and the Cal-27 cell line. Suppression of miR-448 expression attenuated cell proliferation and migration, and induced apoptosis of Cal-27 cells. Furthermore, miR-448 bound with the 3'-untranslated region of metallophosphoesterase domain containing 2 (MPPED2) mRNA, thereby reducing the MPPED2 protein level. Thus, it appears that miR-448 acts as a tumor inducer, causing OSCC growth by inhibiting the expression of its target MPPED2. These results demonstrate that miR-448 plays a critical role in OSCC tumorigenesis, and is a potential therapeutic target.

7.
J Biomed Res ; 292015 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-26273018

RESUMO

Differentially expressed genes are thought to regulate the development and progression of oral squamous cell carcinomas (OSCC). The purpose of this study was to screen differentially expressed mRNAs in OSCC and matched paraneoplastic normal tissues, and to explore the intrinsic mechanism of OSCC development and progression. We obtained the differentially expressed mRNA expression profiles in 10 pairs of fresh-frozen OSCC tissue specimens and matched paraneoplastic normal tissue specimens by high-throughput RNA sequencing. By using Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, the functional significance of the differentially expressed genes were analyzed. We identified 1,120 significantly up-regulated mRNAs and 178 significantly down-regulated mRNAs in OSCC, compared to normal tissue. The differentially expressed mRNAs were involved in 20 biological processes and 68 signal pathways. Compared to adjacent normal tissue, the expression of MAGEA11 was up-regulated; TCHH was down-regulated. These findings were verified by real-time PCR. These differentially expressed mRNAs may function as oncogenes or tumor suppressors in the development and progression of OSCC. This study provides novel insights into OSCC. However, further work is needed to determine if these differentially expressed mRNAs have potential roles as diagnostic biomarkers and candidate therapeutic targets for OSCC.

8.
Nutr Cancer ; 67(1): 68-72, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25298278

RESUMO

Inconclusive information for the role of dairy food intake in relation to ovarian cancer risk may associate with adverse effects of lactose, which has been hypothesized to increase gonadotropin levels in animal models and ecological studies. Up to now, several studies have indicated the association between dairy food intake and risk of ovarian cancer, but no identified founding was reported. We performed this meta-analysis to derive a more precise estimation of the association between dairy food intake and ovarian cancer risk. Using the data from 19 available publications, we examined dairy food including low-fat/skim milk, whole milk, yogurt and lactose in relation to risk of ovarian cancer by meta-analysis. Pooled odds ratio (OR) with 95% confidence interval (CI) were used to assess the association. We observed a slightly increased risk of ovarian cancer with high intake of whole milk, but has no statistical significance (OR = 1.228, 95% CI = 1.031-1.464, P = 0.022). The results of other milk models did not provide evidence of positive association with ovarian cancer risk. This meta-analysis suggests that low-fat/skim milk, whole milk, yogurt and lactose intake has no associated with increased risk of ovarian cancer. Further studies with larger participants worldwide are needed to validate the association between dairy food intake and ovarian cancer.


Assuntos
Dieta/efeitos adversos , Carboidratos da Dieta/efeitos adversos , Lactose/efeitos adversos , Leite/efeitos adversos , Neoplasias Ovarianas/epidemiologia , Iogurte/efeitos adversos , Animais , Dieta com Restrição de Gorduras/efeitos adversos , Carboidratos da Dieta/análise , Feminino , Lactose/análise , Leite/química , Neoplasias Ovarianas/etiologia , Neoplasias Ovarianas/prevenção & controle , Risco , Iogurte/análise
9.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 32(4): 400-3, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25241546

RESUMO

OBJECTIVE: To construct and analyze the differential expression profiles of microRNAs (miRNA) and mRNA in oral squamous cell carcinoma (OSCC). To explore the miRNA and mRNA of OSCC development and progression. METHODS: The differential expression profiles of miRNA and mRNA were built by high-throughput deep sequencing technology. Using Gene Ontology (GO) enrichment analysis, the roles of differentially expressed miRNA and mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis were analyzed. RESULTS: Seventy-seven differentially expressed miRNA and 1 298 differentially expressed mRNAs were identified in OSCC. GO analysis showed that 73 miRNA had found target mRNA in cell cycle, cell proliferation, cell differentiation and cell apoptosis of OSCC. Moreover, a miRNA could regulate multiple mRNA. CONCLUSION: The differential expression profiles of miRNA and mRNA have close relationship with the development and progression of OSCC.


Assuntos
Carcinoma de Células Escamosas , MicroRNAs , Neoplasias Bucais , RNA Mensageiro , Apoptose , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos
10.
Mol Biol Rep ; 41(1): 217-23, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24218166

RESUMO

Our previous studies indicated that a purified rabbit antiserum against X-sperm contained sex-specific antibodies (SSAbs) which preferentially bound to sex-sorted X-sperm. The specificity of sex-specific antiserum was initially demonstrated using flow cytometry only, which resulted in uncertainty. In this study, the putative SSAbs against bovine X-sperm (XSSAb) were produced by a series of immunological approaches, and the effectiveness of separation of sperm using putative XSSAb was validated. Subsequently, the XSSAb was used to immunoprecipitate sex-specific proteins (SSPs) in bovine sperm, followed by two-dimensional gel electrophoresis. The results showed 7.6, 15.2 and 52.1 % of sex-sorted Y-sperm, sex-sorted X-sperm and unsorted sperm were recognized by the neutralized rabbit antisera against X-sperm, respectively. Also the purity of separation of sperm using putative XSSAb reached 74.3 % when the immunologically separated sperm were injected into oocytes. In addition, three candidate SSP sports about 30 kDa were captured by the XSSAb. Our results confirmed that the putative XSSAb contained SSAbs, and implied that these three protein sports might be SSPs in bovine X-sperm. This provides a potentially more efficient method for sorting sperm and lays a foundation for future search for SSPs.


Assuntos
Soros Imunes/química , Proteínas de Membrana/metabolismo , Espermatozoides/fisiologia , Animais , Bovinos , Separação Celular , Eletroforese em Gel Bidimensional , Feminino , Citometria de Fluxo , Imunoprecipitação , Masculino , Proteínas de Membrana/imunologia , Coelhos , Análise para Determinação do Sexo , Cromossomo X/metabolismo , Cromossomo Y/metabolismo
11.
Huan Jing Ke Xue ; 34(1): 98-107, 2013 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-23487924

RESUMO

Sediment quality criterion (SQC) is the concentrations of sediment-associated contaminants that are unlikely to be associated with sediment toxicity or other adverse effects on benthic invertebrates. Thus, the derivation of SQC is crucial to protect benthic invertebrates, and can serve as the tool for scientific sediment management. Sediment, interstitial water, and plant samples were collected at 43 sampling sites from the Xiangjiang River, and metal concentrations were determined. Based on equilibrium partitioning approach, spiked sediment toxicity approach using Hyalella azteca, and background value approach, SQC for Cd and Hg in the Xiangjiang River was derived. Results showed that, SQC-L for Cd and Hg in the Xiangjiang River were 1.89 mg x kg(-1) and 0.13 mg x kg(-1) respectively, and SQC-H were 28.32 mg x kg(-1) and 0.79 mg x kg(-1) respectively. SQC were comparable to those in previous studies. Also, the reasonability of SQC was demonstrated by the heavy metal concentrations in plants, matching sediment chemistry and toxicity data for benthic invertebrates in the Xiangjiang River. To assess the sediment quality of Xiangjiang, metal concentrations in sediment samples were compared with the SQC. It was found that the proportion of sampling sites with Cd and Hg concentrations lower than SQC-L or higher than SQC-H was low, and 74.4% and 76.7% of sampling sites showed Cd and Hg concentrations between SQC-L and SQC-H.


Assuntos
Cádmio/análise , Sedimentos Geológicos/química , Mercúrio/análise , Rios/química , Poluentes Químicos da Água/análise , Animais , Cádmio/toxicidade , China , Monitoramento Ambiental/métodos , Invertebrados/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade
12.
Anim Reprod Sci ; 131(3-4): 129-34, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22516229

RESUMO

This study aims to investigate the effects of follicle stimulating hormone (FSH) in combination with growth and differentiation factor-9 (GDF-9) or basic fibroblast growth factor (bFGF) on the activation, survival and growth of cattle primordial follicles. Ovarian tissues were cultured for 3, 7, 14, 22 days in α minimum essential medium (α-MEM) supplemented with FSH, FSH+GDF-9 or FSH+bFGF. Non-cultured and cultured ovarian fragments were processed for histological and TUNEL analysis. Compared to the FSH medium, the results showed FSH+GDF-9 medium increased the percentage of primary follicles in all culture periods and secondary follicles after 14 days of culture (P<0.05), meanwhile the diameter of primary and secondary follicles were also observed to increase in this medium after 7 days of cultures (P<0.05). FSH+bFGF medium appeared to increase the percentage of primary follicles after 14 days of culture and secondary follicles at day 14 of culture than FSH medium (P<0.05). Furthermore, the FSH+GDF-9 and FSH+bFGF mediums had a greater percentage of normal follicles, and lesser apoptotic cell rates than FSH medium. The results first indicated that FSH in combination with GDF-9 or bFGF can improve the survival, activation, and growth of cattle primordial follicles after the long-term culture of ovarian cortex.


Assuntos
Bovinos/fisiologia , Fator 2 de Crescimento de Fibroblastos/farmacologia , Hormônio Foliculoestimulante/farmacologia , Fator 9 de Diferenciação de Crescimento/farmacologia , Folículo Ovariano/efeitos dos fármacos , Animais , Feminino , Fator 2 de Crescimento de Fibroblastos/administração & dosagem , Hormônio Foliculoestimulante/administração & dosagem , Fator 9 de Diferenciação de Crescimento/administração & dosagem , Técnicas de Cultura de Tecidos
13.
Mol Biol Rep ; 39(6): 7117-23, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22327646

RESUMO

Genes of hypothalamic-pituitary-gonadal axis play a key role in male reproductive performance. This study evaluated the polymorphisms of luteinizing hormone receptor (LHR) and hypothalamic gonadotropin-releasing hormone (GnRH) genes and their effects on sperm quality traits including semen volume per ejaculate (VOL), sperm density (SD), fresh sperm motility (FSM), thawed sperm motility (TSM), acrosome integrity rate (AIR), and abnormal sperm rate (ASR) collected from 205 Chinese Hostein bulls. The study bulls consisted of 205 mature Chinese Holstein, 27 Simmental, 28 Charolais, and 14 German yellow cattle. One single nucleotide polymorphism (SNP) (A883G) in exon 2 of GnRH and two SNPs (A51703G and G51656T) in intron 9 of LHR were identified in 274 bulls. Analysis of variance in 205 Chinese Holstein bulls showed that age had significant effect on both SD and FSM (P < 0.01), and ASR (P < 0.05). With regards to genotype and its interaction with age, only the SNP of G51656T in LHR gene had significant effect on SD (P < 0.05, P < 0.01; respectively). The association result showed that bulls with AG genotype had higher FSM than bulls with AA and GG genotype in LHR at 51,703 locus (P < 0.10), and bulls with GG genotype had higher SD than bulls with TT genotype in LHR at G51656T locus (P < 0.10). Phenotypic correlation among the traits revealed that significant negative correlations were observed between ASR and AIR (r = -0.736, P < 0.01), ASR and AIR (r = -0.500, P < 0.01). There were moderate positive correlations between VOL and SD (r = 0.422, P < 0.01), as well as FSM (r = 0.411, P < 0.01). In conclusion, LHR may be a potential marker for sperm quality of SD and FSM.


Assuntos
Bovinos/genética , Hormônio Liberador de Gonadotropina/genética , Polimorfismo de Nucleotídeo Único , Receptores do LH/genética , Espermatozoides/fisiologia , Acrossomo/fisiologia , Fatores Etários , Animais , Criopreservação , Frequência do Gene , Estudos de Associação Genética , Genótipo , Hipotálamo/metabolismo , Masculino , Polimorfismo de Fragmento de Restrição , Preservação do Sêmen , Análise de Sequência de DNA , Contagem de Espermatozoides , Motilidade Espermática/genética
14.
Anim Reprod Sci ; 126(3-4): 151-6, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21684095

RESUMO

Candidate genes follicle stimulation hormone receptor (FSHR), inhibin alpha (INHA), inhibin bata A (INHBA) and prolactin (PRL) were investigated for their association with sperm quality traits of semen volume per ejaculate (VOL), sperm concentration (SCON), motility (MOT), sperm motility in frozen semen (FMOT), acrosome integrity rate (AIR) and abnormal sperm rate (ASR) in a total of 123 normal mature Holstein bulls. Three reported single nucleotide polymorphisms (SNPs) of FSHR A-234500T (rs43676359), IHNA A192G (rs41257116), and IHNBA C7639T (rs43408735), and 3 novel SNPs (G7550A, C7661T, and T8370C) in exons 4 and 5 of bovine PRL gene (NC 007324) were analyzed. Analysis of variance revealed that FSHR A-234500T and INHBA C7639T polymorphisms significantly associated with VOL (P<0.05) and SCON (P<0.05), and the polymorphism of INHBA C7639T also had significant effects on MOT (P<0.05). Furthermore, the INHA A192G polymorphism significantly associated with AIR (P<0.05). No significant differences were found between the polymorphisms of PRL gene and sperm quality traits.


Assuntos
Bovinos/genética , Subunidades beta de Inibinas/genética , Inibinas/genética , Polimorfismo Genético/genética , Prolactina/genética , Receptores do FSH/genética , Espermatozoides/metabolismo , Animais , Frequência do Gene/genética , Genótipo , Masculino , Polimorfismo de Nucleotídeo Único/genética , Característica Quantitativa Herdável , Sêmen/metabolismo , Motilidade Espermática/genética
15.
Sheng Wu Gong Cheng Xue Bao ; 24(6): 995-8, 2008 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-18807981

RESUMO

In the current work, the fusion gene including somatostatin (SS) and the hepatitis B surface antigen gene was cloned into a balanced lethal system plasmid (pYA3493), and then transformed into asd- attenuated Salmonella choleraesuis C500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and DNA sequencing, designated as pYA-SS. The expression and immunogenicity of fusion protein were detected by SDS-PAGE and Western blot analysis. These results show that the recombinant prokaryotic expression plasmid pYA-SS could express the SS fusion protein with good immunogenicity in C500 strain. In above all, this study could provide reliable materials to develop novel, good and safe vaccine in enhancing the growth of animals.


Assuntos
Fusão Gênica Artificial , Antígenos de Superfície da Hepatite B/genética , Salmonella arizonae/genética , Somatostatina/genética , Somatostatina/imunologia , Animais , Clonagem Molecular , Humanos , Plasmídeos/genética , Células Procarióticas/metabolismo , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Salmonella arizonae/metabolismo , Somatostatina/biossíntese
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