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1.
J Nanobiotechnology ; 19(1): 388, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34823537

RESUMO

BACKGROUND: Esophageal cancer is the fifth most common cancer affecting men in China. The primary treatment options are surgery and traditional radio-chemotherapy; no effective targeted therapy exists yet. Self-assembled RNA nanocarriers are highly stable, easily functionally modified, and have weak off-tumor targeting effects. Thus, they are among the most preferred carriers for mediating the targeted delivery of anti-tumor drugs. miR-375 was found to be significantly down-regulated in esophageal squamous cell carcinoma (ESCC) tissues and its overexpression effectively inhibits the proliferation, migration, and invasion of ESCC cells. Moreover, epidermal growth factor receptor (EGFR) was overexpressed in ESCC cells, and accumulation of RNA nanoparticles in ESCC tumors was enhanced by EGFR-specific aptamer (EGFRapt) modification. RESULTS: Herein, a novel four-way junction RNA nanocarrier, 4WJ-EGFRapt-miR-375-PTX simultaneously loaded with miR-375, PTX and decorated with EGFRapt, was developed. In vitro analysis demonstrated that 4WJ-EGFRapt-miR-375-PTX possesses strong thermal and pH stabilities. EGFRapt decoration facilitated tumor cell endocytosis and promoted deep penetration into 3D-ESCC spheroids. Xenograft mouse model for ESCC confirmed that 4WJ-EGFRapt-miR-375-PTX was selectively distributed in tumor sites via EGFRapt-mediating active targeting and targeted co-delivery of miR-375 and PTX exhibited more effective therapeutic efficacy with low systemic toxicity. CONCLUSION: This strategy may provide a practical approach for targeted therapy of ESCC.

2.
Thorac Cancer ; 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34806328

RESUMO

BACKGROUND: Surgical resection of the thymus is indicated in the presence of primary thymic diseases such as thymoma. Video-assisted thoracoscopic surgery (VATS) and robot-assisted thoracic surgery (RATS) offer a minimally invasive approach to thymectomy. However, there is no clear conclusion whether RATS can achieve an equal or even better surgical effect when compared with VATS in treatment of thymoma. We performed this meta-analysis to explore and compare the outcomes of RATS versus VATS for thymectomy in patients with thymoma. METHODS: PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure (CNKI), Medline, and Web of Science databases were searched for full-text literature citations. The quality of the articles was evaluated using the Newcastle-Ottawa Scale and the data analyzed using Review Manager 5.3 software. Fixed or random effect models were applied according to heterogeneity. Subgroup analysis was conducted. RESULTS: A total of 11 studies with 1418 patients, of whom 688 patients were in the RATS group and 730 in the VATS group, were involved in the analysis. Compared with VATS, RATS was associated with less blood loss in operation, lower volume of drainage, fewer postoperative pleural drainage days, shorter postoperative hospital stay, and fewer postoperative complications. There was no significant difference in operative time and patients with or without myasthenia gravis between the two groups. CONCLUSIONS: RATS has more advantages over VATS, indicating that RATS is better than VATS in terms of postoperative recovery. We look forward to more large-sample, high-quality randomized controlled studies published in the future.

3.
Zhongguo Zhong Yao Za Zhi ; 46(19): 4875-4880, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34738380

RESUMO

Due to the diverse sources and unique structures, the chemical components of Chinese medicinal materials are easy to self-assemble to form nanoparticles. The formation of self-assembled nanoparticles(SAN) can not only affect the absorption and distribution of the effective ingredients in Chinese medicinal materials but also may improve the biological activity of the effective ingredients or their simple mixtures, which is of great significance for revealing the compatibility mechanism of Chinese medicine prescription, developing new Chinese medicine products, and producing new nanomaterials. This paper reviews the formation, isolation, characterization, and application of SAN of Chinese medicines, and discusses the problems and development trends of the relevant research, which can provide reference for the further study and promote the innovation and application of such SAN.


Assuntos
Medicamentos de Ervas Chinesas , Nanopartículas , Medicina Tradicional Chinesa , Prescrições
4.
Ann Surg Oncol ; 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34743281

RESUMO

BACKGROUND: Patient-reported outcomes are critical for obtaining valuable patient insight into different surgical treatment options with comparable clinical outcomes. This study aimed to compare early postoperative patient-reported symptoms and functioning between thoracoscopic segmentectomy and lobectomy for small-sized (≤ 2 cm) peripheral non-small-cell lung cancer (NSCLC). PATIENTS AND METHODS: This study included 110 patients who underwent thoracoscopic segmentectomy or lobectomy for peripheral NSCLC ≤ 2 cm in a multicenter prospective longitudinal study (CN-PRO-Lung 1). Symptom severity, functional status, and short-term clinical outcomes were compared between the groups. Symptom severity and functional status were measured using the MD Anderson Symptom Inventory-Lung Cancer at baseline, daily post-surgery, and weekly post-discharge for up to 4 weeks. Both the proportion of moderate-to-severe scores and mean scores on a 0-10 scale were compared between the groups. RESULTS: Overall, 48 and 62 patients underwent thoracoscopic segmentectomy and lobectomy, respectively. No significant between-group differences were found in the severity of the top five symptoms (coughing, shortness of breath, pain, fatigue, and disturbed sleep) or in the impairment of all six function items (work, walking, general activity, enjoyment of life, mood, and relations with others) during both the 6-day postoperative hospitalization and the 4-week post-discharge (all p > 0.05). Short-term clinical outcomes of postoperative hospital stay, operative time, drainage time, postoperative in-hospital oral morphine equivalent dose, and complication rate were also comparable (all p > 0.05). CONCLUSIONS: In patients with peripheral NSCLC ≤ 2 cm, thoracoscopic segmentectomy and lobectomy might produce comparable symptom burden and functional impairment during the early postoperative period.

5.
J Sep Sci ; 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34739178

RESUMO

The separation of compounds with similar polarities is challenging. In the present study, five flavone glycosides, including two groups with similar polarities, were obtained from Dracocephalum tanguticum by three high-speed counter-current chromatography modes, including flow rate conversion mode, recycling mode, and heart-cut mode. With flow rate conversion mode, compounds 3 and 4 with similar polarities and compound 5 were separated by high-speed counter-current chromatography with ethyl acetate/methanol/water (5.0% acetic acid) (8:2:10, v/v) system. The flow rate was controlled as: 1.8 mL/min for 0-160 min, 2.2 mL/min for 160-200 min, and 2.5 mL/min for 200-400 min. However, compounds 1 and 2 with similar polarities were not separated due to the similar distributive properties. Then, a recycling and heart-cut mode were introduced to improve the separation efficiency. The heart-cut mode was introduced in the second and third cycles, and compounds 1 and 2 were well separated in the fourth cycle. Consequently, five flavone glycosides, including two groups with similar polarities were obtained and identified as cosmosiin (1), pedaliin (2), quercetin-3-O-rutinoside (3), pedaliin-6''-acetate (4), and sorbifolin-6-O-ß-glucopyranoside (5). The current strategy provides a reference for separating compounds with similar polarities from a crude sample.

6.
Int J Med Robot ; : e2352, 2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34773371

RESUMO

BACKGROUND: To present our experiences with partial nephrectomy (PN) through retroperitoneal approach (RP) with the Kangduo robotic system. METHODS: From December 2020 to February 2021, the perioperative data of 11 patients underwent PN through RP with the Kangduo robotic system were collected prospectively. RESULTS: For the R.E.N.A.L. nephrometry score, 72.7% of patients had a low score (4-6) and 27.3% of patients had a medium score (7-9). Seven tumours were posterior (P), four tumours were on the midline (X). All procedures were completed successfully. The median warm ischemia time was 18.5 (IQR, 13.7-21.0) min. None of the patients had positive surgical margins at definitive histology (all pT1a). No high-grade perioperative complications or device-related adverse events occurred. At a mean follow-up of 8 ± 0.8 months, no complications occurred in all patients. CONCLUSIONS: RPPN using the novel Kangduo robotic system is a safe and effective option for managing posterior and lateral renal tumours with R.E.N.A.L. nephrometry scores ≤9.

7.
Ecotoxicol Environ Saf ; 227: 112925, 2021 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-34717216

RESUMO

Microcystin-LR (MC-LR) is one of the most worldwide harmful cyanobacterial toxins. A lots of antibodies against MC-LR have been generated and characterized. However, the knowledge about the epitopes of MC-LR was still limited. The objective of this study was to analyze the epitopes of MC-LR and demonstrate the binding mode of MC-LR with its antibody. The variable genes of a mouse hybridoma cell line (Mab5H1-3B3) raised against MC-LR have been cloned and assembled in a single chain variable fragment (scFv), and then soluble expressed in E.coli BL21. Based on the scFv, the IC50 and IC10 for MC-LR were determined to be 7.45 nM and 0.30 nM by competitive ELISA. And the scFv also showed 115% and 112% cross-reactivities to MC-RR and MC-YR, and 59% to MC-LA. By molecular docking, the binding mode between MC-LR and its scFv was demonstrated. A hydrogen bond interaction was observed between the carbonyl group of Adda5 residue of MC-LR and its scFv, and the guanidyl group of Arg4 residue and phenyl group of Adda5 residue of MC-LR were also involved in the interaction. These predicted epitopes were supported by antibody cross-reactivity data. By comparing the antibody informatics of MC-LR scFv with its predicted paratopes, VH-CDR1 was crucial for MC-LR binding, and its specificity could be tuned by engineering in Vκ-CDR1 and Vκ-CDR3. These information would be useful for the hapten design for microcystins or improving the properties of MC-LR scFv in vitro.


Assuntos
Microcistinas , Animais , Ensaio de Imunoadsorção Enzimática , Epitopos , Toxinas Marinhas , Camundongos , Simulação de Acoplamento Molecular
10.
Huan Jing Ke Xue ; 42(10): 4889-4896, 2021 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-34581132

RESUMO

Taking the Minhang District of Shanghai as a typical rapidly urbanizing area, and based on 595 soil samples from 36 plots, the content of eight heavy metals in soils from five different land uses were analyzed. The ecological risk was evaluated using the Nemerow composite index and the potential ecological risk index. The results showed that the variation coefficients of the heavy metals Zn and Cd were highest, and were notably affected by human activities. The content of heavy metals in industrial land soil was relatively high compared to residential land and cultivated land soils, and heavy metal content was lowest in public management and service land soil. The Nemerow composite index of Zn and Cd was high, corresponding to severe and moderate levels of pollution, respectively. The other heavy metals were found at warning or light levels of pollution. The potential ecological risks posed by heavy metals in the soils from different land uses, in descending order, were heavy-metal-related industry land > non-heavy-metal-related industry land≈residential land > cultivated land≈public management and service land. Industrial land had a greater risk of heavy metal pollution due to industrial operations, complex historical production, and widely variable levels of management. Heavy metal accumulation also tended to occur in residential land and cultivated land soils. Therefore, the prevention of soil heavy metal pollution in association with different land uses, and the control of associated risks during redevelopment, are key challenges in rapidly urbanizing area.


Assuntos
Metais Pesados , Poluentes do Solo , China , Monitoramento Ambiental , Humanos , Metalurgia , Metais Pesados/análise , Medição de Risco , Solo , Poluentes do Solo/análise
11.
Nat Prod Res ; : 1-8, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34587845

RESUMO

A new triterpenoid saponin, 3-O-ß-D-allopyranosyl (1→3)-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-ß-D-glucopyranosyl (1→6)-ß-D-glucopyranosyl ester (IV), together with six known ones Hederacholichiside F (I), Tanguticoside B (II), Tauroside St-H1 (III), Hederoside H1 (V), Kalopanaxsaponin G (VI), Hederasaponin B (VII) were separated from Clematis tangutica (Maxim.) Korsh. Their cytotoxic activities were evaluated. Saponins IV (new compound) and I showed selective inhibitory activities against HGC-27 with IC50 values of 20.17 and 66.18 µM. Saponin VII exhibited extensive inhibitory action against HGC-27, Hela and SK-OV-3 with IC50 values of 16.47-71.36 µM. Saponin III showed selective inhibitory activity against SK-OV-3 with the IC50 value of 48.70 µM. All isolated saponins were inactive (IC50 >150 µM) to GES-1.

12.
J Chromatogr Sci ; 2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34343279

RESUMO

The pomace of sea buckthorn berries is usually discarded when transforming into nonalcoholic or alcoholic beverages, jellies, jams, juices, candies and dairy products. Here, we established a promising approach for one step separation of five flavonoids from the waste pomace of sea buckthorn berries through counter-current chromatography. The crude extract of waste pomace of sea buckthorn berries after juicing was injected into counter-current chromatography with hexane/ethyl acetate/ethanol/water (v/v/v/v, 5:7:5:7) as the solvent system. As a result, five flavonoids, including quercetin, laricitrin, isorhamnetin-7-O-rhamnoside, kaempferol and isorhamnetin, were obtained in a single step separation. Our finding showed that ethanol is a good substitute for methanol to regulate the partition coefficient in hexane/ethyl acetate/ methanol/water system. This study provided a significant measure to utilize the waste pomace of Sea buckthorn berries.

13.
J Cardiothorac Surg ; 16(1): 212, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-34332598

RESUMO

BACKGROUND: There were very few reports of Rosai-Dorfman disease in the thymus, which known as sinus histiocytosis with massive lymphadenopathy. It usually accompanied with other systemic symptoms such as fever, malaise, night sweats, or weight loss in the short term. Case presentation We herein report a rare case of RDD of thymic origin and a review of the literature concerning the clinical and pathological features of this disease, which is often misdiagnosed as thymoma. The patient was underwent thymectomy to extirpate the lesion completely by video-assisted thoracic surgery. CONCLUSIONS: To the best of our knowledge, this is the fourth thymus occurring RDD case with proof via histology and IHC. Our findings suggest the difficulty of making a diagnosis before surgery and more cases will need to be reported in order to facilitate the preoperative diagnosis of such a rare tumor.


Assuntos
Histiocitose Sinusal , Biópsia , Feminino , Histiocitose Sinusal/diagnóstico , Histiocitose Sinusal/cirurgia , Humanos , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Tórax , Tomografia Computadorizada por Raios X
14.
Am J Prev Med ; 61(6): 831-840, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34384653

RESUMO

INTRODUCTION: Breast cancer is the most frequently diagnosed cancer among women worldwide. Previous studies have found a higher risk in nurses but no clear relationship among other health professionals. This study aims to investigate the risks of incident breast cancer among female health professionals compared with that among nonhealth professionals in Taiwan. METHODS: This longitudinal observational cohort study included >35 years (1979-2016) of data from 4 nationwide population-based databases in Taiwan and identified matched cohorts with 1-to-2 propensity score matching between 277,543 health professionals and 555,086 nonhealth professionals. This study calculated total person-years for study subjects and compared breast cancer incidence between matched health and nonhealth professionals. Multivariable Cox proportional hazards and competing risk analyses were used, stratified by birth age, job tenure categories, and types of health professional license. Statistical analyses were conducted in 2019-2020. RESULTS: Health professionals had a significantly higher risk of breast cancer (hazard ratio=1.34, 95% CI=1.28-1.41; subdistribution hazard ratio=1.36, 95% CI=1.30, 1.42). Elevated risk of breast cancer incidence was associated with birth age, job tenure, and several health professional license types, including physician, pharmacist, registered nurse, midwife, medical technologist, and psychologist. CONCLUSIONS: Elevated breast cancer risk was found overall in female health professionals. Regular ultrasonography for younger women and mammography for those aged >45 years may be necessary in the annual labor physical examination for female health professionals.


Assuntos
Neoplasias da Mama , Neoplasias da Mama/epidemiologia , Estudos de Coortes , Feminino , Humanos , Incidência , Estudos Longitudinais , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Risco , Fatores de Risco , Taiwan/epidemiologia
15.
Acta Pharm Sin B ; 11(6): 1648-1666, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34221874

RESUMO

Leading by cytotoxicity against HepG2 cells, bioactivity-guided fractionation of the EtOAc fraction from Artemisia atrovirens led to the isolation of 18 new guaianolide dimers, artematrolides A-R and lavandiolides A, B, C, H, and J. Eight compounds (1, 4, 10, 12, 13, and 19-21) were unambiguously confirmed by the single-crystal X-ray diffraction analyses, and the others were elucidated based on IR, UV, HRESIMS, 1D and 2D NMR experiments, and comparison of the experimental and calculated ECD data. Structurally, all of them were [4 + 2] Diels-Alder adducts of two monomeric guaianolides. The isolates were evaluated for their cytotoxicity against three human hepatoma cell lines, and 19 compounds demonstrated cytotoxicity against HepG2, SMMC-7721, and Huh7 cell lines. Especially, compounds 1, 12, 14, and 15 exhibited cytotoxicity with IC50 values of 4.4, 3.8, 7.6, and 6.7 µmol/L (HepG2), 9.6, 4.6, 6.6, and 6.0 µmol/L (SMMC-7721), and 7.6, 4.5, 6.9, and 5.6 µmol/L (Huh7), respectively. Notably, compound 12 showed the most promising activity against three human hepatoma cell lines and dose-dependently inhibited cell migration and invasion, induced G2/M cell cycle arrest and cell apoptosis in HepG2 cells, down-regulated the expression of BCL-2 and PARP-1, and activated PARP-1 to up-regulate the expression of cleaved-PARP-1.

16.
Gene Ther ; 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34285388

RESUMO

Prostate cancer is one of the most common cancers in men. The heterogeneity and mutations exhibited by prostate cancer cells often results in the progression to incurable metastatic castration-resistant prostate cancer (mCRPC). Our previous investigations demonstrated that the virus-like particles (VLPs) of JC polyomavirus (JCPyV) can deliver exogenous genes to prostate cancer cells for expression. JCPyV VLPs packaging pPSAtk (PSAtk-VLPs) possess the ability to transcriptionally target and selectively induce cytotoxicity in prostate cancer cells in vitro and in vivo, as pPSAtk can only express the thymidine kinase gene, a suicide gene, in androgen receptor-positive cells. To further investigate whether PSAtk-VLPs inhibit the growth of metastasized prostate cancer cells, we established an animal model of bone-metastatic prostate cancer to compare PSAtk-VLPs with leuprorelin acetate and enzalutamide, hormonal agents commonly used in clinical settings, and investigated the effectiveness of PSAtk-VLPs. In the present study, we observed that PSAtk-VLPs effectively inhibited the growth of prostate cancer cells that had metastasized to the bone in the metastatic animal model. In addition, PSAtk-VLPs showed a higher effectiveness than hormone therapy in this animal model study. These results suggest that PSAtk-VLPs may serve as a treatment option for mCRPC therapy in the future.

17.
Trends Cell Biol ; 31(11): 924-939, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34284921

RESUMO

Organelles are critical structures in mediating the assembly and activation of inflammasomes in mammalian cells, resulting in inflammation and cell death. Assembly of inflammasomes can occur at the mitochondria, endoplasmic reticulum, nucleus, trans-Golgi network, or pathogen surface, facilitated by the overarching architecture of the cytoskeleton. NLRP3 and Pyrin inflammasome sensors may form smaller speckles and converge on a single larger speck at the microtubule-organizing center (MTOC). This signaling hub activates multiple mammalian inflammatory and apoptotic caspases, cytokine substrates, the pore-forming protein gasdermin D, and the plasma membrane rupture protein ninjurin-1 (NINJ1), allowing pyroptosis, cellular disintegration, and inflammation to ensue. In this review, we highlight the role of mammalian cell types and organellar architectures in executing inflammasome responses.

18.
Chemosphere ; 285: 131331, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34237501

RESUMO

Floating constructed wetlands (FCWs) have attained tremendous popularity for water purification purposes. However, FCW functions establishment in nutrients removal from carbon-limited wastewater, especially in cold weather, is still a challenge. Here, two drinking water treatment residual (DWTR) based biocarriers (B-I: DWTR cakes, B-II: DWTR cakes combined with woodchips) have been augmented into FCW to enhance the nutrients (N and P) removal performance. Compared to the traditional FCW, the intensified FCWs simultaneously achieved higher N and P removal efficiencies, with average pollutants removal of 52.16 ± 11.51% for TN and 92.72 ± 1.61% for TP in FCW-I and 57.65 ± 9.43% for TN and 92.17 ± 2.55% for TP in FCW-II, respectively, while their removal in FCW-III of 27.74 ± 7.11% for TN and 17.91 ± 9.27% for TP. B-II performed best in overcoming the negative influence of low temperature in nutrients removal. Mass balance budget indicated that most P was enriched in DWTR based biocarriers. Thus it is feasible to recycle and recover P from the surface water. Furthermore, P in the sediment can be changed from active P to stable P, mitigating the internal P release risk. This study can help to expand the understanding of the intensified FCWs and promote the practical application of FCWs.


Assuntos
Purificação da Água , Áreas Alagadas , Carbono , Nitrogênio/análise , Fósforo , Eliminação de Resíduos Líquidos , Águas Residuárias
19.
Bioorg Chem ; 114: 105072, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34144276

RESUMO

Random screening revealed that the EtOH extract of Artemisia atrovirens showed significant cytotoxicity against two human hepatoma cell lines (HepG2 and Huh7) with the inhibitory ratio of 98.9% and 99.7% at the concentration of 100 µg/mL. Further bioactivity-guided isolation of active fraction led to 16 new guaiane-type sesquiterpenoids, artematrovirenins A-P (1-16). Their structures were elucidated by extensive spectroscopic data. The absolute stereochemistry of compounds 1 and 14 was determined by single-crystal X-ray diffraction analyses. Pharmacological evaluation suggested that five compounds (3, 5, 8, 10, and 15) exhibited cytotoxicity, compounds 3 and 5 displayed cytotoxicity against HepG2 cell line with an IC50 values of 8.0 and 16.0 µM, as well as against Huh7 cell line with values of 18.2 and 32.2 µM.

20.
Sci Rep ; 11(1): 11889, 2021 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-34088940

RESUMO

The ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For tissue-specific targeting in this study, JCPyV VLPs were conjugated with a specific peptide for bladder cancer (SPB) that specifically binds to bladder cancer cells. The suicide gene thymidine kinase was packaged and delivered by SPB-conjugated VLPs (VLP-SPBs). Expression of the suicide gene was detected only in human bladder cancer cells and not in lung cancer or neuroblastoma cells susceptible to JCPyV VLP infection in vitro and in vivo, demonstrating the target specificity of VLP-SPBs. The gene transduction efficiency of VLP-SPBs was approximately 100 times greater than that of VLPs without the conjugated peptide. JCPyV VLPs can be specifically guided to target particular cell types when tagged with a ligand molecule that binds to a cell surface marker, thereby improving gene therapy.

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