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1.
Microbiome ; 12(1): 35, 2024 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-38378577

RESUMO

BACKGROUND: Haemaphysalis longicornis is drawing attentions for its geographic invasion, extending population, and emerging disease threat. However, there are still substantial gaps in our knowledge of viral composition in relation to genetic diversity of H. longicornis and ecological factors, which are important for us to understand interactions between virus and vector, as well as between vector and ecological elements. RESULTS: We conducted the meta-transcriptomic sequencing of 136 pools of H. longicornis and identified 508 RNA viruses of 48 viral species, 22 of which have never been reported. Phylogenetic analysis of mitochondrion sequences divided the ticks into two genetic clades, each of which was geographically clustered and significantly associated with ecological factors, including altitude, precipitation, and normalized difference vegetation index. The two clades showed significant difference in virome diversity and shared about one fifth number of viral species that might have evolved to "generalists." Notably, Bandavirus dabieense, the pathogen of severe fever with thrombocytopenia syndrome was only detected in ticks of clade 1, and half number of clade 2-specific viruses were aquatic-animal-associated. CONCLUSIONS: These findings highlight that the virome diversity is shaped by internal genetic evolution and external ecological landscape of H. longicornis and provide the new foundation for promoting the studies on virus-vector-ecology interaction and eventually for evaluating the risk of H. longicornis for transmitting the viruses to humans and animals. Video Abstract.


Assuntos
Ixodidae , Phlebovirus , Carrapatos , Animais , Humanos , Ixodidae/genética , Viroma/genética , Filogenia , Phlebovirus/genética
2.
Int J Parasitol Parasites Wildl ; 23: 100912, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38375444

RESUMO

Soft ticks (Ixodida: Argasidae) are ectoparasites of terrestrial vertebrates with worldwide distributions. As one representative group of Argasidae, the genus Argas has an important vectorial role in transmitting zoonotic diseases. However, our knowledge of the subgenus Argas in China is still limited, as most literature only lists occurrence records or describes specific case reports without providing detailed morphological characteristics and further molecular data. This study aims to characterize Argas vulgaris through complete mitochondrial sequencing and morphological diagnostic techniques based on a batch of adult specimens collected from Ningxia Hui Autonomous Regions (NXHAR), North China. The morphology and microstructures of Ar. vulgaris and other lectotypes of argasid ticks in the subgenus Argas were also observed using a stereomicroscope. Following DNA extraction and sequencing, a complete mitochondrial sequence of Ar. vulgaris was assembled and analyzed within a phylogenetic context. The 14,479 bp mitogenome of Ar. vulgaris consists of 37 genes, including 13 genes for protein coding, two for ribosomal RNA, 22 for transfer RNA, and one for control region (D-loops). Phylogenetic analysis of Ar. vulgaris showed 98.27%-100% nucleotide identity with Ar. japonicus, indicating a close relationship between the two tick species. The morphological diagnostic features to differentiate Ar. vulgaris from other ticks within the subgenus Argas included the location of the anus and setae on the anterior lip of the female genital aperture. This study provided high-resolution scanning electron microscope images of female Ar. vulgaris and corresponding molecular data, representing valuable resources for future accurate species identification.

3.
Sci Data ; 11(1): 218, 2024 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-38368451

RESUMO

As an important forestry pest, Coronaproctus castanopsis (Monophlebidae) has caused serious damage to the globally valuable Gutianshan ecosystem, China. In this study, we assembled the first chromosome-level genome of the female specimen of C. castanopsis by merging BGI reads, HiFi long reads and Hi-C data. The assembled genome size is 700.81 Mb, with a scaffold N50 size of 273.84 Mb and a contig N50 size of 12.37 Mb. Hi-C scaffolding assigned 98.32% (689.03 Mb) of C. Castanopsis genome to three chromosomes. The BUSCO analysis (n = 1,367) showed a completeness of 91.2%, comprising 89.2% of single-copy BUSCOs and 2.0% of multicopy BUSCOs. The mapping ratio of BGI, second-generation RNA, third-generation RNA and HiFi reads are 97.84%, 96.15%, 97.96%, and 99.33%, respectively. We also identified 64.97% (455.3 Mb) repetitive elements, 1,373 non-coding RNAs and 10,542 protein-coding genes. This study assembled a high-quality genome of C. castanopsis, which accumulated valuable molecular data for scale insects.


Assuntos
Agricultura Florestal , Genoma de Inseto , Hemípteros , Feminino , Cromossomos , Ecossistema , Filogenia , RNA , Hemípteros/genética
4.
Microbiol Spectr ; 11(4): e0030123, 2023 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-37260375

RESUMO

Theileria, a tick-borne intracellular protozoan, can cause infections of various livestock and wildlife around the world, posing a threat to veterinary health. Although more and more Theileria species have been identified, genomes have been available only from four Theileria species to date. Here, we assembled a whole genome of Theileria luwenshuni, an emerging Theileria, through next-generation sequencing of purified erythrocytes from the blood of a naturally infected goat. We designated it T. luwenshuni str. Cheeloo because its genome was assembled by the researchers at Cheeloo College of Medicine, Shandong University, China. The genome of T. lunwenshuni str. Cheeloo was the smallest in comparison with the other four Theileria species. T. luwenshuni str. Cheeloo possessed the fewest gene gains and gene family expansion. The protein count of each category was always comparable between T. luwenshuni str. Cheeloo and T. orientalis str. Shintoku in the Eukaryote Orthologs annotation, though there were remarkable differences in genome size. T. luwenshuni str. Cheeloo had lower counts than the other four Theileria species in most categories at level 3 of Gene Ontology annotation. Kyoto Encyclopedia of Genes and Genomes annotation revealed a loss of the c-Myb in T. luwenshuni str. Cheeloo. The infection rate of T. luwenshuni str. Cheeloo was up to 81.5% in a total of 54 goats from three flocks. The phylogenetic analyses based on both 18S rRNA and cox1 genes indicated that T. luwenshuni had relatively low diversity. The first characterization of the T. luwenshuni genome will promote better understanding of the emerging Theileria. IMPORTANCE Theileria has led to substantial economic losses in animal husbandry. Whole-genome sequencing data of the genus Theileria are currently limited, which has prohibited us from further understanding their molecular features. This work depicted whole-genome sequences of T. luwenshuni str. Cheeloo, an emerging Theileria species, and reported a high prevalence of T. luwenshuni str. Cheeloo infection in goats. The first assembly and characterization of T. luwenshuni genome will benefit exploring the infective and pathogenic mechanisms of the emerging Theileria to provide scientific basis for future control strategies of theileriosis.


Assuntos
Theileria , Theileriose , Animais , Bovinos , Theileria/genética , Filogenia , Cabras , Genômica
5.
Zhongguo Zhen Jiu ; 41(5): 553-6, 2021 May 12.
Artigo em Chinês | MEDLINE | ID: mdl-34002573

RESUMO

A new type of handshape tuina-cupping instrument is developed to overcome the shortcomings of traditional cupping therapy, such as indentation and pain, and simulates the tuina manipulation, so as to realize the combination of cupping therapy and tuina manipulation. The handshape tuina-cupping instrument has a trumpet-shaped, silica gel edge. At the same time, the finger-belly shape is added at the cupping edge to simulate the shape of fingers during tuina. Under the action of air suction and deflation of pulsating airflow, the purpose of simulating tuina manipulation is achieved, and the indentation and pain caused by the handshape tuina-cupping instrument is significantly less than that of the traditional cupping instrument.


Assuntos
Massagem , Medicina Tradicional Chinesa , Humanos , Dor , Tecnologia
6.
Mol Pain ; 17: 1744806920988443, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33478334

RESUMO

Recent reports suggest pain from surgical injury may influence the risks associated with exposure to opioids. In mice, hind-paw incision attenuates morphine-primed reinstatement due to kappa opioid receptor activation by dynorphin. In this focused group of studies, we examined the hypotheses that kappa-opioid receptor activation in the nucleus accumbens mediates attenuated drug- primed reinstatement after incisional surgery, and the G-protein biased mu-opioid agonist, oliceridine, leads to less priming of the dynorphin effect in comparison to morphine. To address these hypotheses, adult C57BL/6 male mice underwent intracranial cannulation for administration of the selective kappa-opioid antagonist norBNI directly into the nucleus accumbens. After recovery, they were conditioned with morphine or oliceridine after hind-paw incisional injury, then underwent extinction followed by opioid-primed reinstatement. Intra-accumbal administration of norBNI was carried out prior to testing. The nucleus accumbens and medial prefrontal cortex were extracted and analyzed for expression of prodynorphin. We observed that animals conditioned with morphine in the setting of incisional injury demonstrated blunted responses to opioid-primed reinstatement, and that the blunted responses were reversed with intra-accumbal norBNI administration. Persistently elevated levels of prodynorphin expression in the medial prefrontal cortex and nucleus accumbens were observed in the incised morphine-treated animals. However, both behavioral and molecular changes were absent in animals with incisional injury conditioned with oliceridine. These findings suggest a role for prodynorphin expression in the nucleus accumbens with exposure to morphine after surgery that may protect individuals from relapse not shared with biased mu- opioid receptor agonists.

7.
Sheng Li Xue Bao ; 72(5): 631-642, 2020 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-33106833

RESUMO

The aim of the present study was to investigate the effects of exercises with different durations and intensities on mitochondrial autophagy and FUNDC1 in rat skeletal muscles. Sixty male Sprague-Dawley rats were randomly divided into 2- and 4-week control groups (Con), moderate-intensity exercise groups (M-ex groups, treadmill exercise, 16 m/min, 1 h/d, 6 d/week), and high-intensity exercise groups (Hi-ex groups, treadmill exercise, 35 m/min, 20 min/d, 6 d/week). The bilateral soleus muscles were separated after the intervention, and paraffin sections were prepared for transmission electron microscopy. ELISA method was used to detect the content of citrate synthase (CS). The co-localizations of microtubule-associated protein 1 light chain 3 (LC3)/cytochrome c oxidase IV (COX-IV), FUNDC1/COX-IV and LC3/FUNDC1 were observed by immunofluorescent staining in frozen sections. The skeletal muscle mitochondria were extracted, and the expression of autophagy-related proteins, including AMPKα, p-AMPKα, Unc-51 like kinase 1 (ULK1), FUNDC1, LC3 and p62, were detected by Western blot. The results showed that exercise increased mitochondrial function, i.e. peroxisome proliferator-activated receptor γ co-activator-1α (PGC-1α), COX-I protein expression levels and CS content. There was no difference of mitochondrial function parameters between 2-week M-ex and 2-week Hi-ex groups, while mitochondrial function of 4-weeks Hi-ex group was significantly lower than that of 4-week M-ex group. Under the same exercise intensity, mitochondrial autophagy activation in skeletal muscle of 4-week exercise was higher than that in 2-week exercise group; Under the same duration of exercise, mitochondrial autophagy activation of Hi-ex group was higher than that in M-ex group. Both 2- and 4-week exercise intervention increased LC3/COX-IV, COX-IV/FUNDC1, and FUNDC1/LC3 co-localizations. Exercise increased LC3-II/LC3-I ratio, down-regulated p62 protein expression level, up-regulated FUNDC1, ULK1 protein expression levels and AMPKα phosphorylation, and the changes of these proteins in 4-week Hi-ex group were significantly greater than those in 4-week M-ex group. These results suggest exercise induces mitochondrial autophagy in skeletal muscles, and the activity of autophagy is related to the duration and intensity of exercise. The induction mechanism of exercise may involve the mediation of FUNDC1 expression through AMPK-ULK1 pathway.


Assuntos
Autofagia , Mitocôndrias , Animais , Terapia por Exercício , Humanos , Masculino , Proteínas de Membrana/metabolismo , Proteínas de Membrana/fisiologia , Proteínas Mitocondriais/metabolismo , Proteínas Mitocondriais/fisiologia , Músculo Esquelético/metabolismo , Ratos , Ratos Sprague-Dawley
8.
Chemosphere ; 185: 361-367, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28709040

RESUMO

Bisphenol A (BPA) is one of the most prevalent chemicals in many products used on a daily basis, making human exposure to it incredibly pervasive and raising concerns about its health consequences. One area of research focus has been the role of BPA exposure in promoting the development of ovarian cancer; however, the doses used in most of previous studies are relatively high and most likely exceed physiologically relevant levels. At the same time, few studies have described potential mechanisms underlying the link between BPA and increased cancer risk. To address these concerns we investigated the mechanism(s) by which low concentrations of BPA promote proliferation and energy metabolism in the human ovarian cancer cell line OVCAR-3. We found that even sub-toxic BPA concentrations not only drove increased OVCAR-3 cell proliferation but also promoted glycolysis-based metabolism, as evidenced by elevated cell viability, accelerated cell proliferation, increased levels of intracellular ATP, lactate, and pyruvic acid. Importantly, all of these effects were estrogen receptor α (ERα) dependent, as siRNA-mediated ERα silencing decreased BPA-induced proliferation, pinpointing the crucial role of ERα-conducted signaling in BPA-induced biological effects. Together, our findings revealed a new mechanism through which BPA promoted cell proliferation by reinforcing glycolysis-based energy production dependent on ER signaling. This study would thus open a new path to understand BPA-induced biological effects on tumor cells.


Assuntos
Compostos Benzidrílicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Receptor alfa de Estrogênio/metabolismo , Glicólise/efeitos dos fármacos , Neoplasias Ovarianas/patologia , Fenóis/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Estrogênios não Esteroides/farmacologia , Feminino , Humanos , Neoplasias Ovarianas/metabolismo , Receptores de Estrogênio/metabolismo , Transdução de Sinais/efeitos dos fármacos
9.
J Int Med Res ; 45(2): 533-539, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28415931

RESUMO

Objective The ideal agents for conscious sedation during ambulatory inguinal hernia repair are still unclear. We aimed to compare the analgesic, sedative, haemodynamic, and side effects of dexmedetomidine with those of propofol in combination with fentanyl for conscious sedation in patients undergoing inguinal hernia repair. Methods Eighty patients undergoing unilateral inguinal hernia repair were prospectively randomized to receive either dexmedetomidine (n = 40) or propofol (n = 40). Dexmedetomidine and propofol dosages were adjusted to maintain the targeted level of sedation. Results After administration of sedative drugs, patients who received dexmedetomidine had a significantly lower heart rate. The intraoperative requirement of fentanyl was significantly lower in patients who received dexmedetomidine compared with patients who received propofol. Administration of dexmedetomidine was associated with a reduced postoperative pain score, longer time for onset of sedation, and a slightly longer recovery time. No serious adverse events occurred in either group. The patients' overall satisfaction score was comparable between the two groups. Conclusion Dexmedetomidine is an effective adjuvant when co-administered with fentanyl for conscious sedation in patients who undergo inguinal hernia repair. Administration of dexmedetomidine decreases the requirement of fentanyl and the pain score, but slightly prolongs the time to sedation and recovery.


Assuntos
Analgésicos não Narcóticos , Anestésicos Intravenosos , Sedação Consciente/métodos , Dexmedetomidina , Hérnia Inguinal/cirurgia , Dor Pós-Operatória/prevenção & controle , Propofol , Idoso , Idoso de 80 Anos ou mais , Combinação de Medicamentos , Feminino , Fentanila , Frequência Cardíaca/efeitos dos fármacos , Hérnia Inguinal/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória/fisiopatologia , Satisfação do Paciente/estatística & dados numéricos , Estudos Prospectivos
10.
Int J Biol Macromol ; 91: 926-33, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27318110

RESUMO

Arginine kinase plays an important role in cellular energy metabolism and is closely related to the environmental stress response in marine invertebrates. We studied the Cu(2+)-mediated inhibition and aggregation of Sepia pharaonis arginine kinase (SPAK) and found that Cu(2+) markedly inhibited the SPAK activity along with mixed-type inhibition against the arginine substrate and noncompetitive inhibition against the ATP cofactor. Spectrofluorimetry results showed that Cu(2+) induced a tertiary structure change in SPAK, resulting in exposure of the hydrophobic surface and increased aggregation. Cu(2+)-mediated SPAK aggregation followed first-order kinetics consistent with monophasic and a biphasic processes. Addition of osmolytes, including glycine and proline, effectively blocked SPAK aggregation and restored SPAK activity. Our results demonstrated the effects of Cu(2+) on SPAK catalytic function, conformation, and aggregation, as well as the protective effects of osmolytes on SPAK folding. This study provided important insights into the role of Cu(2+) as a negative effector of the S. pharaonis metabolic enzyme AK and the possible responses of cephalopods to unfavorable environmental conditions.


Assuntos
Arginina Quinase/química , Arginina Quinase/metabolismo , Agregados Proteicos/efeitos dos fármacos , Sepia/enzimologia , Trifosfato de Adenosina/farmacologia , Animais , Arginina Quinase/antagonistas & inibidores , Dicroísmo Circular , Ativação Enzimática/efeitos dos fármacos , Glicina/farmacologia , Cinética , Prolina/farmacologia , Inibidores de Proteínas Quinases/farmacologia , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Espectrometria de Fluorescência
11.
Exp Ther Med ; 11(4): 1259-1264, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27073433

RESUMO

Interleukin-13 (IL-13), a Th2 cytokine, plays an important role in fibrosis, inflammation, tissue hyperresponsiveness and tumor development. Although studies have demonstrated that IL-13 exerts its roles through signal transducer and activator of transcription 6 (STAT6) signaling pathway, recent studies have revealed that I kappa B kinase (IKK)/nuclear factor kappa B (NFκB) pathway may also be involved in. The aim of this study was to investigate whether IL-13 delivers signals to IKKß/NFκBp65 and whether autophagy genes are IL-13-induced the activation of NFκBp65 transcriptional targets in fibroblasts of breast tumor stroma. We examined the phosphorylation of IKKß, the activation of NFκBp65 and NFκBp65-targeted autophagy genes in fibroblasts co-cultured with breast cancer cells under the condition of IL-13 stimulation. Results of this study showed that IL-13 induced IKKß phosphorylation in the fibroblast line ESF co-cultured with breast cancer cell line BT474, and subsequently NFκBp65 was activated and aimed at beclin 1 and microtubule-associated protein 1 light chain 3 B (MAP1LC3B or LC3B) in these ESF cells. BMS345541, an inhibitor of IKK/NFκB pathway, significantly inhibited the IL-13-induced the activation of NFκB and also inhibited NFκB-targeted beclin 1 and LC3B expression. Our results suggest that IL-13 regulates beclin 1 and LC3B expression through IKKß/NFκBp65 in fibroblasts co-cultured with breast cancer cells, and IL-13 plays role in activating IKKß/NFκBp65.

12.
Mol Med Rep ; 13(1): 744-52, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26647977

RESUMO

Reduced expression levels of caveolin­1 (Cav­1) in tumor stromal fibroblasts influences the occurrence and progression of tumors, particularly in breast cancer, but the relevant molecular mechanism is unclear. The present study aimed to clarify the potential mechanism underlying the promotion of tumor growth by reduced Cav­1 expression levels, by investigating Cav­1­targeted molecules in fibroblasts and breast cancer cells. The expression of growth factors in the ESF fibroblast cell line transfected with Cav­1 small interfering RNA (siRNA) was examined. The expression of apoptotic regulators in the BT474 breast cancer cell line that was co­cultured with the fibroblasts, was also investigated. The transfection of Cav­1­targeting siRNA in ESF cells resulted in efficient and specific inhibition of Cav­1 expression. The downregulation of Cav­1 increased the expression and secretion of stromal cell­derived factor­1 (SDF­1), epidermal growth factor (EGF) and fibroblast­specific protein­1 (FSP­1) in ESF cells. This resulted in the accelerated proliferation of the breast cancer cells. Tumor protein 53­induced glycolysis and apoptosis regulator (TIGAR) was upregulated in the BT474 cells under the condition of co­culture with Cav­1 siRNA fibroblasts, while levels of reactive oxygen species (ROS) were decreased, resulting in apoptosis inhibition in the breast cancer cells. These results demonstrated that the downregulation of Cav­1 promoted the growth of breast cancer cells through increasing SDF­1, EGF and FSP­1 in tumor stromal fibroblasts, and TIGAR levels in breast cancer cells. To the best of our knowledge, the present study supports the hypothesis that Cav­1 possesses tumor­suppressor properties, with the mechanism of Cav­1­dependent signaling involving the regulation of SDF­1, EGF, FSP­1 and TIGAR.


Assuntos
Caveolina 1/metabolismo , Regulação para Baixo , Fibroblastos/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/genética , Regulação para Cima , Apoptose , Proteínas Reguladoras de Apoptose , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Quimiocina CXCL12/genética , Quimiocina CXCL12/metabolismo , Técnicas de Cocultura , Fator de Crescimento Epidérmico/genética , Fator de Crescimento Epidérmico/metabolismo , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Monoéster Fosfórico Hidrolases , Interferência de RNA , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/metabolismo , Proteína A4 de Ligação a Cálcio da Família S100
13.
Int J Clin Exp Med ; 8(4): 4883-98, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26131062

RESUMO

BACKGROUND: There are still no absolute parameters predicting progression of adenoma into cancer. The present study aimed to characterize functional differences on the multistep carcinogenetic process from the adenoma-carcinoma sequence. METHODS: All samples were collected and mRNA expression profiling was performed by using Agilent Microarray high-throughput gene-chip technology. Then, the characteristics of mRNA expression profiles of adenoma-carcinoma sequence were described with bioinformatics software, and we analyzed the relationship between gene expression profiles of adenoma-adenocarcinoma sequence and clinical prognosis of colorectal cancer. RESULTS: The mRNA expressions of adenoma-carcinoma sequence were significantly different between high-grade intraepithelial neoplasia group and adenocarcinoma group. The biological process of gene ontology function enrichment analysis on differentially expressed genes between high-grade intraepithelial neoplasia group and adenocarcinoma group showed that genes enriched in the extracellular structure organization, skeletal system development, biological adhesion and itself regulated growth regulation, with the P value after FDR correction of less than 0.05. In addition, IPR-related protein mainly focused on the insulin-like growth factor binding proteins. CONCLUSION: The variable trends of gene expression profiles for adenoma-carcinoma sequence were mainly concentrated in high-grade intraepithelial neoplasia and adenocarcinoma. The differentially expressed genes are significantly correlated between high-grade intraepithelial neoplasia group and adenocarcinoma group. Bioinformatics analysis is an effective way to study the gene expression profiles in the adenoma-carcinoma sequence, and may provide an effective tool to involve colorectal cancer research strategy into colorectal adenoma or advanced adenoma.

14.
Medicine (Baltimore) ; 94(1): e211, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25569640

RESUMO

Bisphenol A (BPA), a carbon-based synthetic compound, exhibits hormone-like properties and is present ubiquitously in the environment and in human tissues due to its widespread use and biological accumulation. BPA can mimic estrogen to interact with estrogen receptors α and ß, leading to changes in cell proliferation, apoptosis, or migration and thereby, contributing to cancer development and progression. At the genetic level, BPA has been shown to be involved in multiple oncogenic signaling pathways, such as the STAT3, MAPK, and PI3K/AKT pathways. Moreover, BPA may also interact with other steroid receptors (such as androgen receptor) and plays a role in prostate cancer development. This review summarizes the current literature regarding human exposure to BPA, the endocrine-disrupting effects of BPA, and the role of BPA in hormone-associated cancers of the breast, ovary, and prostate.


Assuntos
Compostos Benzidrílicos/toxicidade , Neoplasias da Mama/induzido quimicamente , Disruptores Endócrinos/toxicidade , Exposição Ambiental/efeitos adversos , Neoplasias Ovarianas/induzido quimicamente , Fenóis/toxicidade , Neoplasias da Próstata/induzido quimicamente , Feminino , Humanos , Masculino
15.
J Med Microbiol ; 64(Pt 4): 390-393, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25596116

RESUMO

There is limited information regarding the molecular epidemiology and antifungal susceptibilities of Candida isolates using the Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and the D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using the Neo-Sensitabs tablet diffusion method. Candida species were isolated from 186 cases (61.79 %). The most common pathogen was Candida albicans (91.4 %), followed by Candida glabrata (4.3 %), Candida tropicalis (3.2 %) and Candida parapsilosis (1.1 %). The susceptibility rates to C. albicans were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole, ketoconazole and terbinafine (P<0.01). The resistance rates to C. albicans were 4.7, 6.5, 7.1, 7.6, 12.3, 27.7 and 74.7 % for caspofungin, miconazole, itraconazole, voriconazole, fluconazole, ketoconazole and terbinafine, respectively. No drugs tested apart from fluconazole exhibited differences in resistance between C. albicans and non-albicans Candida isolates. The results demonstrate that, using DNA sequencing, C. albicans is the most common isolate from Chinese patients with VVC. Caspofungin, voriconazole and fluconazole may be preferable to other azoles and terbinafine in the treatment of VVC.


Assuntos
Antifúngicos/farmacologia , Candida/classificação , Candida/efeitos dos fármacos , Candidíase Vulvovaginal/microbiologia , Técnicas Microbiológicas/métodos , Técnicas de Diagnóstico Molecular/métodos , Adolescente , Adulto , Candida/genética , Candida/isolamento & purificação , China , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Farmacorresistência Fúngica , Feminino , Humanos , Pessoa de Meia-Idade , Dados de Sequência Molecular , RNA Ribossômico/genética , Análise de Sequência de DNA , Adulto Jovem
16.
Analyst ; 139(23): 6290-7, 2014 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-25285335

RESUMO

Compared to the nitrogen-reaction based pH optical responsive compounds, oxygen-reaction related pH sensors have attracted less attention. In this paper, hemicyanine based pH probes are designed by establishing the equilibrium between phenolate and phenol, and their reversible absorption and emission responses towards pH are evaluated. The indolium-phenol based tetramethylene hemicyanine (1a) has colorimetric responses at 455 and 578 nm due to the protonating and deprotonating processes; its emission spectra shows ratiometric changes at 594 and 654 nm with large Stokes shifts under acidic (139 nm) and basic conditions (76 nm). The bromide substituent of the hemicyanine (1b) has a lower pKa value compared with unsubstituted hemicyanine (1a), which suggests that adjustable pKa can be achieved by the modification of electron withdrawing groups. The theoretical calculations based on the density functional theory (DFT) were also used to explain the optical properties. Moreover, the in cellulo fluorescence imaging shows that the hemicyanine (1a) can be used for the detection of intracellular pH levels.


Assuntos
Carbocianinas/química , Colorimetria/métodos , Fenóis/química , Concentração de Íons de Hidrogênio , Sondas Moleculares/química , Estrutura Molecular
17.
Asian Pac J Trop Med ; 7(10): 787-91, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25129461

RESUMO

OBJECTIVES: To explore the effect of Fibulin-5 expression on cell proliferation and invasion in human gastric cancer patients. METHODS: Fibulin-5 expression was detected in 56 samples of surgically resected gastric cancer and paired noncancerous tissues using qRT-PCR and immunoblotting. Fibulin-5 was knocked down by Fibulin-5 shRNA in MGC-803 cells, then BrdU cell proliferation and transwell invasion assays were used to determine cell proliferation and invasion. RESULTS: The level of Fibulin-5 mRNA in gastric cancer tissues was significantly higher as compared with that in normal tumor-adjacent tissues (P<0.05). Otherwise, the level of Fibulin-5 protein in cancer and noncancerous tissues was consistent with mRNA expression (P<0.05). Fibulin-5 protein expression in tumor tissues with poorly differentiated, lymph node metastasis and advanced TNM tumor stage was significantly higher (P<0.05, respectively). Fibulin-5 was obviously knocked down by Fibulin-5 shRNA (P<0.05), and Fibulin-5 knockdown significantly inhibited cell proliferation and invasion in MGC-803 cells (P<0.05, respectively). CONCLUSIONS: The high-expression of Fibulin-5 is associated with the malignant clinicopathologic parameters in gastric cancer and Fibulin-5 knockdown inhibits cell proliferation and invasion in MGC-803 cells, suggesting Fibulin-5 may act as a key factor in the progression of gastric cancer.

18.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 27(4): 395-8, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21481315

RESUMO

AIM: To investigate the effect of alcohol and/or IL-13 on the expression of collagen type I alpha 1 chain gene (COL1A1, COL3A1) mRNA and collagen production of human lung fibroblast(HFL-1). METHODS: HFL-1 was cultured and real time RT-PCR was used to determine the expression of IL-13 receptor (IL-13Rα1, IL-13Rα2, IL-4Rα) mRNA, COL1A1 mRNA and COL3A1 mRNA. ELISA was used to determine the production of collagen type I. RESULTS: Low level of Alcohol (25, 50, 100, 200 mmol/L) greatly up-regulated the expression of IL-13Rα1 mRNA and IL-4Rα mRNA of HFL-1, but IL-13Rα2 mRNA expression was decreased. low level of alcohol (25, 50, 100, 200 mmol/L) had no effect on the expression of COL1A1 and COL3A1. IL-13 (10, 20, 50 µg/L) stimulated the expression of COL1A1 and COL3A1 in a dose-dependent manner. The expression of COL1A1 and COL3A1 induced by combination of alcohol (200 mmol/L) and IL-13(10 µg/L, 20 µg/L, 50 µg/L) was significantly higher than IL-13(10, 20, 50 µg/L) alone. Combination of alcohol (200 mmol/L) and IL-13(10, 20, 50 µg/L) could significantly increase collagen typeI production than IL-13(10, 20, 50 µg/L) alone. CONCLUSION: Low level of alcohol has no effect on the mRNA expression of COL1A1 and COL3A1 of HFL-1cells, but has great effect on the mRNA expression of IL-13Rα1, IL-13Rα2 and IL-4Rα. Combination of alcohol and IL-13 significantly up-regulated the collagen production and expression of COL1A1 and COL3A1 of HFL-1 cells.


Assuntos
Colágeno/genética , Etanol/farmacologia , Fibroblastos/efeitos dos fármacos , Interleucina-13/farmacologia , Fibrose Pulmonar/etiologia , Células Cultivadas , Cadeia alfa 1 do Colágeno Tipo I , Ensaio de Imunoadsorção Enzimática , Fibroblastos/metabolismo , Humanos , Subunidade alfa1 de Receptor de Interleucina-13/genética , Subunidade alfa2 de Receptor de Interleucina-13/genética , Pulmão/citologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa
19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 25(1): 16-9, 2009 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-19126381

RESUMO

AIM: To observe the inhibitory effects of tyrosine kinases inhibitor A77 1726 on collagen generation induced by IL-13 in fibroblasts. METHODS: The inhibition rate of fibroblast proliferation with different concentration of A77 1726 was observed by MTT method. The fibroblasts were divided into the experimental group (A77 1726 50 micromol/L and IL-13 100 microg/L) and the control group (IL-13 100 microg/L). After 24, 48 and 72 hours, the inhibitory effects of A77 1726 on collagen secretion of fibroblasts investigated by hydroxyproline release assay. The inhibitory effects of A77 1726 on collagen type I alpha1 gene mRNA expression in fibroblasts were examinated by RT-PCR. The influence of A77 1726 on collagen type I synthesis in fibroblasts was analyzed by Western blot. RESULTS: The proliferation of fibroblasts was inhibited by A77 1726. Total collagen generation was down-regulated after 48 h and 72 h stimulation of A77 1726 (P<0.05). The expression level of collagen type I alpha1 gene mRNA was obviously lower in the experimental group than in the control group after 48 h and 72 h stimulation of A77 1726 (P<0.05). Collagen type I production of fibroblasts treated with A77 1726 for 48 and 72 hours was decreased obviously in the experimental group than in the control group (P<0.05). CONCLUSION: Tyrosine kinases inhibitor A77 1726 has an inhibitory effect on collagen protein synthesis in fibroblasts induced by IL-13.


Assuntos
Compostos de Anilina/farmacologia , Colágeno Tipo I/metabolismo , Inibidores Enzimáticos/farmacologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Hidroxibutiratos/farmacologia , Interleucina-13/farmacologia , Proteínas Tirosina Quinases/antagonistas & inibidores , Células 3T3 , Animais , Western Blotting , Colágeno Tipo I/genética , Crotonatos , Camundongos , Nitrilas , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Toluidinas
20.
Zhonghua Shao Shang Za Zhi ; 24(2): 97-8, 2008 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-18785406

RESUMO

OBJECTIVE: To study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene. METHODS: The plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR. RESULTS: The drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation. CONCLUSION: bla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.


Assuntos
Acinetobacter baumannii/genética , Farmacorresistência Bacteriana Múltipla/genética , Plasmídeos , beta-Lactamases/genética , Infecções por Acinetobacter , Acinetobacter baumannii/enzimologia , Acinetobacter baumannii/isolamento & purificação , Queimaduras/microbiologia , Genes Bacterianos , Humanos , Testes de Sensibilidade Microbiana
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