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1.
Talanta ; 221: 121653, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076168

RESUMO

Rapid detection of cell viability is of importance for various aspects of basic and applied research. However, the most commonly used MTT assay suffers from some disadvantages of time-consuming, poor solubility of formazan crystals, and interference of cell extracts, resulting in either delayed results or inconsistent cell viability. Accumulating evidences show that mitochondrial activity is closely associated with cell proliferation and apoptosis, making it valuable for real-time monitoring of cell fates. Herein, we present a novel type of cell-permeant mitochondrial dyes, composed of coumarin-quinoline conjugates (CQCs), for assessing cell viability through monitoring mitochondrial dynamic changes. CQCs exhibited superior preference for mitochondria, and could sensitively monitor their fissions under pathological conditions. Particularly, CQCs could accurately assess the cell viability within 10 min, allowing for rapidly evaluating therapeutic effects with high sensitivity and accuracy, in comparison of MTT assay. Therefore, CQCs provide a proof-of-concept assay that is appropriately practical either independently or in complementation with other assays for the analysis of cellular viability in broad range of biomedical applications.

2.
EMBO J ; : e105896, 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33140861

RESUMO

COVID-19 is characterised by dysregulated immune responses, metabolic dysfunction and adverse effects on the function of multiple organs. To understand host responses to COVID-19 pathophysiology, we combined transcriptomics, proteomics, and metabolomics to identify molecular markers in peripheral blood and plasma samples of 66 COVID-19 patients experiencing a range of disease severities and 17 healthy controls. A large number of expressed genes, proteins, metabolites and extracellular RNAs (exRNAs) exhibit strong associations with various clinical parameters. Multiple sets of tissue-specific proteins and exRNAs varied significantly in both mild and severe patients suggesting a potential impact on tissue function. Chronic activation of neutrophils, IFN-I signalling as well as a high level of inflammatory cytokines were observed in patients with severe disease progression. In contrast, COVID-19 patients experiencing milder disease symptoms showed robust T cell responses. Finally, we identified genes, proteins and exRNAs as potential biomarkers that might assist in predicting the prognosis of SARS-CoV-2 infection. These data refine our understanding of the pathophysiology and clinical progress of COVID-19.

3.
Int Immunopharmacol ; : 107152, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33187908

RESUMO

RNA virus infection activates the RIG-I-like Receptor (RLR) signaling pathway to produce type I interferons (IFNs), the key components of the antiviral immune response. Forkhead box O1 (FoxO1) is a host transcription factor that participates in multiple biological processes. In this study, FoxO1 was identified as a critical negative regulator of RIG-I-triggered signaling. FoxO1 promoted Sendai virus (SeV) replication and downregulated type I IFN production. Upon SeV infection, FoxO1 suppressed K63-linked ubiquitination of TRAF3 and the interaction between TRAF3 and TBK1, after which the production of type I IFNs via the interferon regulatory transcription factor 3 (IRF3) pathways was reduced. In addition, FoxO1 destabilized IRF3 by facilitating E3 ligase TRIM22- or TRIM21-mediated K48-linked ubiquitination of IRF3. Moreover, the inhibitory effect of FoxO1 was found to depend on its DNA binding domain (DBD). Thus, our findings highlight novel important roles of FoxO1 in controlling RLR-mediated antiviral innate immunity.

4.
J Hazard Mater ; : 124393, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33199141

RESUMO

The present research systematically investigates the atenolol (ATL) degradation in integrated UV/Ozone (O3)/peroxymonosulfate (PMS) process focusing on the synergistic mechanism, reaction kinetics, pollutant degradation pathway and antibacterial activity. The results manifested that the integrated UV/O3/PMS process showed the noteworthy superiority to ATL degradation compared with UV/PMS, UV/O3 and O3/PMS systems. Simultaneously, the impacts of operating parameters like PMS dosage, initial ATL concentration, solution pH and water matrix were comprehensively explored. The ATL elimination efficiency increased linearly with PMS dose and significantly enhanced in alkaline conditions. The •OH and SO4•- were the primary reactive radicals for ATL oxidation in UV/O3/PMS system based on the radical scavenging experiments and electron paramagnetic resonance characterization. Besides, a simplified kinetic model on the basis of the dominant reactions and the steady-state assumption was established to foretell the relative contributions of reactive oxidants for ATL elimination in UV/O3/PMS process. Main transformation products were determined via UPLC-QTOF-MS to infer the possible degradation pathways of ATL. Furthermore, the UV/O3/PMS process could distinctly mitigate the antibacterial activity of ATL and its intermediates to E. coli and B. subtilis. Our findings may have critical implications for the development of novel oxidation processes for recalcitrant contaminants mitigation in water purification.

6.
Clin Lab ; 66(11)2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33180424

RESUMO

BACKGROUND: To establish the reference intervals of GIR, HOMA, and QUICKI index and to identify the clinical value of the three indexes for newly diagnosed diabetes mellitus. METHODS: The results of fasting glucose and insulin were acquired for 123 healthy individuals using Roche cobas-8000 to establish reference intervals of GIR, HOMA, and QUICKI based on Clinical and Laboratory Standards Institute (CLSI) EP28-A3. Meanwhile, 36 newly diagnosed type 1 and type 2 diabetes mellitus (DM) patients were enrolled to judge the effect of insulin resistance/insufficiency using the three indexes based on clinical initial treat-ment procedures. All the data were acquired from Wangjing Hospital, China Academy of Traditional Chinese Medicine. RESULTS: The reference intervals of GIR, HOMA, and QUICKI were 5.83 - 21.15, 0.87 - 4.22, and 0.309 - 0.392, respectively. Concerning to GIR, HOMA, and QUICKI, there were 57.7% (15/26), 80.8% (21/26), and 80.8% (21/26) outside of the reference limit among type 2 DM patients, respectively; The area under the curve (AUC) of the GIR > 10.937, HOMA < 5.436, and QUICKI > 0.299 were 0.937 (95% CI 0.681 - 1.000), 0.689 (95% CI 0.510 - 0.868), and 0.689 (95% CI 0.510 - 0.868) by ROC curves when insulin insufficiency was judged based on whether insulin was included in initial treatment procedures. There concordance rates were 77.8% (28/36), 50% (18/36), and 50% (18/36) using the three indexes, GIR, HOMA, and QUICKI, respectively. CONCLUSIONS: We established reference intervals for GIR, HOMA, and QUICKI. HOMA and QUICKI were more reliable indexes to identify insulin resistance among type 2 DM patients, but GIR was a more reliable index to identify insulin relatively or absolutely insufficiency than HOMA and QUICKI among DM patients.

7.
Biomed Res Int ; 2020: 2739560, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33204690

RESUMO

Objective: This paper included a retrospective review of the effect of tranexamic acid (TXA) combined with pressure bandaging on hemostasis of patients who received a unilateral total knee arthroplasty (TKA) from 2017 to 2019. Methods: A total of 197 patients undergoing TKA were chosen to be classified into 2 groups, the compression bandage control group and compression bandage combined with TXA observation group. The patients received blood routine examination when they were in the 1st, 3rd, and 6th days of before and postoperation. Some parameters, such as hemoglobin (Hb), C-reactive protein (CRP), D-dimer value, fibrinogen, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), international normalized ratio (INR), and erythrocyte sedimentation rate (ESR), were also investigated. Results: In our research, the mean age was 66.54 ± 7.95 years. No difference was found in patient sex (P = 0.876) and age (P = 0.749) between groups. No differences were found in the levels of Hb, fibrinogen, TT, and INR between the 2 groups at each period (P > 0.05). The difference of PT was significantly different on the 1st day (P = 0.011), 3rd day (P = 0.010), and 6th day (P = 0.004) after surgery. Besides, the changes in APTT in observation group were clearly higher compared with the control group on the 3rd day (P = 0.001) and 6th day (P = 0.001). On the 3rd and 6th days after operation, the CRP level of the two groups increased continuously, and the CRP level was significantly higher in the observation group in comparison with the control group (P = 0.008, P = 0.010). On 1st and 3rd days after surgery, compared to the control group, the D-dimer level of patients in the observation group was distinctly fewer (P = 0.001, P = 0.027). Conclusion: TXA combined with compression bandage is a potential option for the reduction of bleeding after TKA.

8.
Ultrasonics ; 110: 106289, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33130363

RESUMO

PURPOSE: Colonoscopy accompanied with biopsy works as the routine endoscopic strategy for the diagnosis of colorectal cancer (CRC) in clinic; however, the colonoscopy is limited to the tissue surface. During the last decades, enabling technologies are emerging to complement with the colonoscopy for better administration of CRC. The conventional low-frequency (<12 MHz) endoscopic ultrasound (EUS) guided fine-needle aspiration (FNA) has been widely used to assess the lesion penetration. With the high-frequency ultrasound transducer (>20 MHz), EUS allows more precise visualization of the colorectal abnormalities. In order to achieve the accurate detection or in situ characterization of the colorectal lesions, the EUS diagnosis needs more patho-physiological related information in the micro-structural or molecular level. Quantitative ultrasound (QUS) technique, which could extract the micro-structural information from the ultrasound radio-frequency (RF) signal, is promising for the non-invasive tissue characterization. To date, the knowledge of the high-frequency endoscopic QUS for the CRC characterization has not been fully determined. METHODS: In this work, to our best knowledge, it is the first application of the QUS technique based on a customized high-frequency EUS system (30.5 MHz center frequency) to characterize the colorectal malignancies in a VX2 rabbit CRC model. To eliminate the response from the ultrasound electronic system and transducer, the ultrasound signals from colon tissue were calibrated. And, the resulting quasi-liner ultrasound spectra were fit by the linear regression test. As a result, three spectral parameters, including the slope (k), intercept (I) and Midband Fit (M), were obtained from the best-fit line. The three spectral parameters were compared between the malignant tissue regions and adjacent normal tissue regions of the colon tissue specimen ex vivo. The independent t-test was conducted between the three parameters from the normal and malignant group. The statistical method of Fisher Linear Discriminant (FLD) was used to explore the linear combinations of the three parameters, so as to provide more tissue micro-structural features than the single parameter alone. The three FLD values were derived from three different combinations among k, I and M. The threshold was selected from the statistical analysis to optimize the differentiation criteria between the malignant and the normal tissues. The color-coded images were used to display the local FLD values and combined with the EUS B-mode image. RESULTS AND CONCLUSIONS: The mean Midband Fit (M) and intercept (I) showed significant differences between the malignant and normal tissue regions. The statistical analysis showed that there were significant differences in all the mean FLD values of the spectral parameter combinations (kI, kM and IM) (t test, P < 0.05). And, the combined image result from the B-mode image and color-coded image could visually correlate with the histology result. In conclusion, the high-frequency endoscopic QUS technique was potential to be used as a complementary method to distinguish the colorectal malignancies by leveraging its morphological and micro-structural ultrasound information.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33090587

RESUMO

Electrochemically generated chemiluminescence, or so-called electrochemiluminescence (ECL), is a powerful transduction technique in biosensing and diagnostics, while mechanistic studies are still scarce. Herein we report the combined use of microtube electrode (MTE) and microscopy to measure the thickness of ECL layer (TEL) to decipher reaction mechanisms. For the classical system involving tris(2,2'-bipyridyl)ruthenium and tri-n-propylamine, the ECL pattern generated at the MTE tends to change from ring to spot upon increasing the luminophore concentration, with the TEL varying from ~3.1 µm to >4.5 µm. This variation is rationalized to arise from the contribution of so-called "catalytic route". While using 2-(dibutylamino)ethanol as the coreactant, the ECL pattern remains ring-shaped and independent on the luminophore concentration. The TEL in this case is ~2.1 µm, implying that ECL generation is always surface-confined. So MTEs can act as optical rulers for measuring the TEL and providing insightful mechanistic information.

10.
Front Immunol ; 11: 580968, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013934

RESUMO

Non-alcoholic fatty liver disease (NAFLD) has been on a global rise. While animal models have rendered valuable insights to the pathogenesis of NAFLD, discrepancy with patient data still exists. Since non-alcoholic steatohepatitis (NASH) involves chronic inflammation, and CD4+ T cell infiltration of the liver is characteristic of NASH patients, we established and characterized a humanized mouse model to identify human-specific immune response(s) associated with NAFLD progression. Immunodeficient mice engrafted with human immune cells (HIL mice) were fed with high fat and high calorie (HFHC) or chow diet for 20 weeks. Liver histology and immune profile of HIL mice were analyzed and compared with patient data. HIL mice on HFHC diet developed steatosis, inflammation and fibrosis of the liver. Human CD4+ central and effector memory T cells increased within the liver and in the peripheral blood of our HIL mice, accompanied by marked up-regulation of pro-inflammatory cytokines (IL-17A and IFNγ). In vivo depletion of human CD4+ T cells in HIL mice reduced liver inflammation and fibrosis, but not steatosis. Our results highlight CD4+ memory T cell subsets as important drivers of NAFLD progression from steatosis to fibrosis and provides a humanized mouse model for pre-clinical evaluation of potential therapeutics.

11.
Anal Chim Acta ; 1134: 34-40, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33059864

RESUMO

Urinary 5-hydroxyindole-3-acetic acid (5-HIAA) is a marker for diagnosis of patients with carcinoid tumors. In clinical practice, a simple colorimetric assay has widely been utilized for urinary 5-HIAA via its reaction with 1-nitroso-2-naphthol (NNa) in nitrous acid medium. However, this colorimetric assay has been criticized for lack of specificity. Herein, we proposed a novel SERS-based method for selective detection of urinary 5-HIAA by the use of a modified nitrosonaphthol reaction, in which hydrochloric acid is substituted for nitric acid to control the reaction conditions. This slight modification is crucial to be able to detect the cationic products in the acidic reaction mixture, because the chloride-aggregated Ag colloids not only create enormous hot spots for SERS enhancement, but also improve the chemical stability of nanostructured Ag substrates in acidic environment. Under optimized conditions, the present method can detect 5-HIAA within 12 min at concentrations as low as 1.2 ng/mL, which is 1000 times lower than that of conventional colorimetric method. And more importantly, the present method exhibits specific response toward 5-HIAA against other metabolites with similar structures in the urine. The relative standard deviation (RSD) of the present method is less than 5%, suggesting the acceptable reproducibility. The recoveries ranging from 89.6% to 106.3% were obtained for spiked human urine samples with RSD of 3.7-4.9%. Furthermore, several healthy person's urine samples were also analyzed using the present method, and experimental results are in compliance with the levels recorded in a healthy population. On the basis of these results we can conclude that the present SERS-based method can provide a valuable alternative to conventional colorimetric assay for clinical diagnosis, evaluating prognosis, and monitoring of treatment in carcinoid tumors.

12.
Arch Microbiol ; 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33104820

RESUMO

An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl. It was catalase-positive and oxidase-negative. The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences. The phylogenetic tree based on the phylogenomic analysis also showed the same results. The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively. The characteristic diamino acid of strain Hz 6-5T was found to be lysine. The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids. The DNA G + C content of isolate Hz 6-5T was 60.8%. Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp. nov. is proposed. The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).

14.
Pathogens ; 9(10)2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33065983

RESUMO

Morels (Morchella spp.) are popular edible fungi with significant economic and scientific value. However, white mold disease, caused by Paecilomyces penicillatus, can reduce morel yield by up to 80% in the main cultivation area in China. Paecilomyces is a polyphyletic genus and the exact phylogenetic placement of P. penicillatus is currently still unclear. Here, we obtained the first high-quality genome sequence of P. penicillatus generated through the single-molecule real-time (SMRT) sequencing platform. The assembled draft genome of P. penicillatus was 40.2 Mb, had an N50 value of 2.6 Mb and encoded 9454 genes. Phylogenetic analysis of single-copy orthologous genes revealed that P. penicillatus is in Hypocreales and closely related to Hypocreaceae, which includes several genera exhibiting a mycoparasitic lifestyle. CAZymes analysis demonstrated that P. penicillatus encodes a large number of fungal cell wall degradation enzymes. We identified many gene clusters involved in the production of secondary metabolites known to exhibit antifungal, antibacterial, or insecticidal activities. We further demonstrated through dual culture assays that P. penicillatus secretes certain soluble compounds that are inhibitory to the mycelial growth of Morchella sextelata. This study provides insights into the correct phylogenetic placement of P. penicillatus and the molecular mechanisms that underlie P. penicillatus pathogenesis.

15.
PLoS Comput Biol ; 16(10): e1008336, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33090994

RESUMO

Genome-wide association studies (GWAS) have primarily identified trait-associated loci in the non-coding genome. Colocalization analyses of SNP associations from GWAS with expression quantitative trait loci (eQTL) evidence enable the generation of hypotheses about responsible mechanism, genes and tissues of origin to guide functional characterization. Here, we present a web-based colocalization browsing and testing tool named LocusFocus (https://locusfocus.research.sickkids.ca). LocusFocus formally tests colocalization using our established Simple Sum method to identify the most relevant genes and tissues for a particular GWAS locus in the presence of high linkage disequilibrium and/or allelic heterogeneity. We demonstrate the utility of LocusFocus, following up on a genome-wide significant locus from a GWAS of meconium ileus (an intestinal obstruction in cystic fibrosis). Using LocusFocus for colocalization analysis with eQTL data suggests variation in ATP12A gene expression in the pancreas rather than intestine is responsible for the GWAS locus. LocusFocus has no operating system dependencies and may be installed in a local web server. LocusFocus is available under the MIT license, with full documentation and source code accessible on GitHub at https://github.com/naim-panjwani/LocusFocus.

16.
Sci Rep ; 10(1): 17673, 2020 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-33077778

RESUMO

Nature reserves play an extraordinarily important role in conserving animal populations and their habitats. However, landscape change and unreasonable zoning designations often render these protected areas inadequate. Therefore, regular evaluation of the efficacy of protected lands is critical for maintaining and improving management strategies. Using species distribution models and GAP analysis, we assessed the changes in suitable habitat for the Brown Eared-pheasant (Crossoptilon mantchuricum) in two Chinese nature reserves between 1995 and 2013. Our results showed that the habitat suitability of Brown Eared-pheasant has changed dramatically during this period, and fragmentation analyses showed an increase in concentration area and decrease in patch area. In particular, our findings show that the national nature reserves need to adjust their ranges to ensure the conservation of this flagship species. Our study further provides a new viewpoint for evaluating the efficacy of protected lands, particularly in highly urbanized regions where conservation goals must be balanced with changing landscapes.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33128144

RESUMO

The design of stable and highly efficient photocatalysts had emerged as an economic and promising way for eliminating harmful pharmaceutical pollutants. In this study, a series of Ag2O-modified g-C3N4 composites with different Ag2O amounts (denoted as Ag2O-CNx) were fabricated via a facile reflux condensation methodology. Ofloxacin (OFL) was chosen as a model pollutant to evaluate the degradation efficiency of the photocatalytic system. The optimal photocatalytic activity was achieved with Ag2O-CN1.0, which reached up to 99.1% removal of OFL after 15-min reaction and the pseudo-first-order constant was 0.469 min-1, approximately 42 times higher than that of g-C3N4. Considering the complexity of the actual environment, the important influential factors such as catalyst dosage, initial OFL concentration, solution pH, and natural organic matter on the OFL degradation were systematically investigated. Additionally, Ag2O-CN1.0 showed good stability and recyclability in multiple cycle experiments. The feasible photodegradation mechanism of OFL was proposed with radical scavenger experiments, and the degradation products were determined. Furthermore, the enhanced photocatalytic activity could be ascribed to not only the high photogenerated charge separation efficiency and the surface plasmon resonance effect of metallic Ag, but also the p-n heterojunction formed between Ag2O and g-C3N4. Therefore, Ag2O-CN1.0 was a treatment material possessing great application prospects for eliminating OFL in wastewater.

18.
Int J Biol Macromol ; 165(Pt B): 2640-2648, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33122059

RESUMO

Our previous study has revealed that Pseudomonas plecoglossicida JUIM01 produced 2-keto-d-gluconic acid (2KGA) and re-utilized 2KGA as an alternative carbon source to support cell growth after complete consumption of glucose. Phosphorylation of intracellular 2KGA to 2-keto-6-phosphogluconic acid by 2-ketogluconate kinase (KguK) was regarded as the first step of 2KGA catabolism in Pseudomonas cytoplasm. In the present study, a kguK gene encoding 2-ketogluconate kinase from P. plecoglossicida JUIM01 was cloned and heterologously expressed in Pichia pastoris. The recombinant KguK showed the highest activity at 30-33 °C and pH 7.7, and high stability at 33 °C. Under the optimal conditions of 30 °C and pH 7.7 with addition of 5 mM Mg2+, the purified and concentrated (~30 folds) KguK had a specific activity of 3649.6 U/g and a Michaelis constant for 2KGA of 8.7 × 10-4 M. Knockout of kguK could retard but not completely inhibit 2KGA catabolism, indicating other existing 2KGA utilization pathway(s). The kguK-knockout P. plecoglossicida significantly reduced 2KGA re-utilization without negative effects on cell growth, glucose consumption or 2KGA production. The outputs demonstrated kguK knockout could be an effective strategy to develop the alternative 2KGA high-producing Pseudomonas strains to avoid the decrease of 2KGA yield caused by its re-utilization.

19.
JMIR Mhealth Uhealth ; 8(10): e22137, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33084588

RESUMO

BACKGROUND: The potential effectiveness of integrated management in further improving the prognosis of patients with atrial fibrillation has been demonstrated; however, the best strategy for implementation remains to be discovered. OBJECTIVE: The aim of this study was to ascertain the feasibility of implementing integrated atrial fibrillation care via the Hospital-Community-Family-Based Telemedicine (HCFT-AF) program. METHODS: In this single-arm, pre-post design pilot study, a multidisciplinary teamwork, supported by efficient infrastructures, provided patients with integrated atrial fibrillation care following the Atrial fibrillation Better Care (ABC) pathway. Eligible patients were continuously recruited and followed up for at least 4 months. The patients' drug adherence, and atrial fibrillation-relevant lifestyles and behaviors were assessed at baseline and at 4 months. The acceptability, feasibility, and usability of the HCFT-AF technology devices and engagement with the HCFT-AF program were assessed at 4 months. RESULTS: A total of 73 patients (mean age, 68.42 years; 52% male) were enrolled in November 2019 with a median follow up of 132 days (IQR 125-138 days). The patients' drug adherence significantly improved after the 4-month intervention (P<.001). The vast majority (94%, 64/68) of indicated patients received anticoagulant therapy at 4 months, and none of them received antiplatelet therapy unless there was an additional indication. The atrial fibrillation-relevant lifestyles and behaviors ameliorated to varying degrees at the end of the study. In general, the majority of patients provided good feedback on the HCFT-AF intervention. More than three-quarters (76%, 54/71) of patients used the software or website more than once a week and accomplished clinic visits as scheduled. CONCLUSIONS: The atrial fibrillation-integrated care model described in this study is associated with improved drug adherence, standardized therapy rate, and lifestyles of patients, which highlights the possibility to better deliver integrated atrial fibrillation management. TRIAL REGISTRATION: Clinicaltrials.gov NCT04127799; https://clinicaltrials.gov/ct2/show/NCT04127799.

20.
Medicine (Baltimore) ; 99(43): e21933, 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33120727

RESUMO

BACKGROUND: Traditional Chinese medicine (TCM) compound formulations are selected according to different populations, with strong targeting and less adverse reactions. As a complex disease, amyotrophic lateral sclerosis (ALS) has limited efficacy in the use of conventional treatment regiments, short life cycle, high cost, many side effects, and low quality of life. It is urgent to seek new alternative therapies. Clinical practice shows that Chinese herbal compound combined with western medicine has certain therapeutic advantages, but there is no evidence of evidence-based medicine. The purpose of this study was to evaluate the efficacy and safety of Chinese herbal compound combined with western medicine in the treatment of ALS. METHODS: Use computer to retrieve English database (PubMed, Embase, Web of Science, the Cochrane Library) and Chinese database (CNKI, Wanfang Database, Weipu database, and China Biomedical Literature Service System), moreover manually retrieve Baidu academic and Google academic from the establishment of the database to 2020 July for randomized controlled clinical study on ALS treated with compound Chinese medicine with western medicine therapy, 2 researchers independently conducted data extraction and literature quality evaluation on the quality of the included studies, and meta-analysis of the included literature was carried out using RevMan5.3 software. RESULTS: This study evaluated the efficacy and safety of TCM combined with western medicine in the treatment of ALS by means of effective rate, improved Norris scale, ALS Functional Rating Scale, TCM syndrome score, and adverse reaction incidence. CONCLUSION: This study will provide reliable evidence for the clinical application of Chinese herbal compound combined with western medicine in the treatment of ALS.OSF registration number: DOI 10.17605/OSF.IO/R5XG4.


Assuntos
Esclerose Amiotrófica Lateral/terapia , Medicamentos de Ervas Chinesas/uso terapêutico , Metanálise como Assunto , Revisões Sistemáticas como Assunto , Terapia Combinada , Humanos , Medicina Tradicional Chinesa , Projetos de Pesquisa
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