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1.
Lasers Med Sci ; 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404885

RESUMO

Early detection of cervical lesions, accurate diagnosis of cervical lesions, and timely and effective therapy can effectively avoid the occurrence of cervical cancer or improve the survival rate of patients. In this paper, the spectra of tissue sections of cervical inflammation (n = 60), CIN (cervical intraepithelial neoplasia) I (n = 30), CIN II (n = 30), CIN III (n = 30), cervical squamous cell carcinoma (n = 30), and cervical adenocarcinoma (n = 30) were collected by a confocal Raman micro-spectrometer (LabRAM HR Evolution, Horiba France SAS, Villeneuve d'Ascq, France). The Raman spectra of six kinds of cervical tissues were analyzed, the dominant Raman peaks of different kinds of tissues were summarized, and the differences in chemical composition between the six tissue samples were compared. An independent sample t test (p ≤ 0.05) was used to analyze the difference of average relative intensity of Raman spectra of six types of cervical tissues. The difference of relative intensity of Raman spectra of six kinds of tissues can reflect the difference of biochemical components in six kinds of tissues and the characteristic of biochemical components in different kinds of tissues. The classification models of cervical inflammation, CIN I, CIN II, CIN III, cervical squamous cell carcinoma, and cervical adenocarcinoma were established by using a support vector machine (SVM) algorithm. Six types of cervical tissues were classified and identified with an overall diagnostic accuracy of 85.7%. This study laid a foundation for the application of Raman spectroscopy in the clinical diagnosis of cervical precancerous lesions and cervical cancer.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33430588

RESUMO

Enhanced emission and adjustable wavelength for single luminogen systems are highly desirable in the scope of photoluminescent materials. Herein, a supramolecular strategy has been proposed for supramolecular assembly-induced enhanced emission and valid emission manipulation by fabricating an amphiphilic copolymer host material with pillar[5]arene units as the side chains, whereby cyanovinylene-based (CV) derivatives are anchored to the polymer hosts via host-guest interactions. The guest-bearing copolymers can further form luminescent supramolecular polymer nanoparticles (SPNs). Remarkably, the as-prepared SPNs exhibit dramatic emission enhancement and tunable fluorescence wavelength, ascribing to the synergetic effects involving the restriction of intramolecular motions and the prevented excimer formation for CV moieties, as endowed by host-guest interactions and the entanglement of the polymer chains. Furthermore, the SPNs can be established as efficient artificial light-harvesting systems via the inclusion of Nile red into the particles for broadened emission spectra. As a proof-of-concept study, the use of pillar[5]arene-containing polymer hosts largely facilitates the emission enhancement and wavelength adjustment for the inherent luminogens, setting the basis for the supramolecular design of highly tunable luminescent systems.

3.
Curr Neuropharmacol ; 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33430733

RESUMO

Chlorogenic acid (CGA) is a kind of traditional Chinese medicine, abundant in honeysuckle and eucommia, has a wide range of biological activities, and pharmacological effects. Previous studies have shown that CGA can regulate learning, memory, cognitive ability, coupled with improvement to anxiety, depression, and other post-traumatic stress disorder (PTSD)-like symptoms. This article explores the protective effects of CGA on neurons, through its anti-apoptotic effect, inhibition of neuroinflammation and oxidative stress, which may be the mechanisms of its improvement of PTSD-like symptoms. It may provide a new therapeutic strategy for the treatment of PTSD and its comorbidities.

4.
Coron Artery Dis ; 2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33471466

RESUMO

BACKGROUND: C-reactive protein (CRP) has been proposed as a contributor to the pathogenesis of coronary artery disease (CAD) and inflammatory reactions, which are associated with a decrease in serum albumin, and it has been reported that the CRP-to-serum albumin ratio (CAR) can predict CAD severity in inpatient ischemic cardiomyopathy (ICM) patients. However, the relationship between the CAR and long-term adverse outcomes in CAD patients after percutaneous coronary intervention (PCI) is still unknown. METHODS: A total of 3561 CAD patients enrolled in the Outcomes and Risk Factors of Patients with Coronary Heart Disease after PCI: an investigation based on case records and follow-up (CORFCHD-ZZ), a retrospective cohort study conducted from January 2013 to December 2017, and 1630 patients meeting the study inclusion criteria were divided into two groups based on the CAR (CAR < 0.186; n = 1301 and CAR ≥ 0.186; n = 329). The primary outcome was long-term mortality, including all-cause mortality (ACM) and cardiac mortality. The average follow-up time was 37.59 months. RESULTS: We found that there were significant differences between the two groups in the incidences of ACM (P < 0.001) and cardiac mortality (P = 0.003). Cox multivariate regression analyses demonstrated that CAR was an independent predictor of ACM [hazard ratio, 2.678; (95% confidence interval (CI), 1.568-4.576); P < 0.001] and cardiac mortality (hazard ratio, 2.055; 95% CI, 1.056-3.998; P = 0.034) in CAD patients after PCI. CONCLUSION: This study revealed that the CAR is an independent and novel predictor of long-term adverse outcomes in CAD patients who have undergone PCI.

5.
BMJ Open ; 11(1): e040182, 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33468526

RESUMO

OBJECTIVE: Hyperbilirubinemia is one of the most common clinical symptoms in newborns. To improve patient outcomes, evidence-based and implementable guidelines are required. However, clinical guidelines may vary in quality, criteria and recommendations among regions and countries. In this study, we aimed to systematically assess the quality of guidelines using the Appraisal of Guidelines for Research & Evaluation (AGREE)-II instrument and summarise the specific recommendations for neonatal hyperbilirubinemia in order to provide suggestions for future guideline development. DESIGN: Systematic review. INTERVENTIONS: We searched the PubMed, Embase, Medline and guideline databases for relevant articles on 10 April 2020. The studies were screened by two independent reviewers according to our inclusion criteria. Two reviewers independently extracted the descriptive data. Four appraisers assessed the guidelines using the AGREE-II instrument. RESULTS: Our systematic review appraised 12 clinical practice guidelines for the diagnosis and management of neonatal hyperbilirubinemia. The 12 guidelines achieved an average score of 36%-89%. The guidelines received the highest scores for clarity of presentation and lowest scores for rigour of development. Most recommendations for diagnosis were relatively consistent, but recommendations regarding risk factors, the initiating threshold of treatment and pharmacotherapy varied. CONCLUSIONS: Our study revealed that current guidelines vary in the quality of the developing process and are inconsistent with regards to recommendations. Future guidelines should afford more attention to the quality of methodologies in guideline development, and more qualified evidence is needed to standardise the initiating threshold of treatment for neonatal hyperbilirubinemia.

6.
Trop Anim Health Prod ; 53(1): 106, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33417104

RESUMO

In the present study, the feasibility of sugarcane top (ST) application in the goat's diet was evaluated. A total of 20 goats were randomly divided into four groups. The dietary treatments were set as follows: animals were fed with king grass (KG), KG with exogenous enzymes (KGE), ST, and ST with exogenous enzymes (STE). The animals were given free access to feed and water. After 15 days of adaptation and 60 days of the experiment, the growth performance, plasma parameters, and rumen microbiota of goats were assessed. The results showed that the KG, ST, and exogenous enzyme supplement had no significant effects on the growth performance and plasma parameters. The diet affected the rumen microbiota diversity and structure, and the alpha and beta diversity in the animals fed with ST were dramatically greater compared with the animals fed with KG. The abundances of Proteobacteria, Cyanobacteria, and Elusimicrobia were significantly decreased in the animals fed with KG or KGE, while the abundances of Firmicutes and Euryarchaeota were significantly higher in the animals fed with KG or KGE. Furthermore, the microbial communities were also different at the genus level. Moreover, the exogenous enzymes had a slight effect on rumen microbiota. Linear discriminant analysis effect size (LEfSe) analysis showed that the greatest differences were found in bacterial taxa, and these specific taxa could be used as biomarkers to distinguish rumen microbiota. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) functional profile prediction indicated that the dietary treatments of ST and KG could also alter the gene expression pattern in nutrient and energy metabolism, as well as replication and repair of genetic information pathways. Collectively, the dietary treatments of KG and ST in the absence or presence of exogenous enzymes had similar effects on the growth performance and plasma parameters of goats. Besides, the KG and ST diets could affect the rumen microbiota community and function of goats. Therefore, ST could be used as a promising alternative feed resource for ruminants without the addition of exogenous enzymes in tropical regions.

7.
J Sci Food Agric ; 2021 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-33421121

RESUMO

BACKGROUND: Fermentation, a traditional food preserving technique and an effective process, widely used to enrich the nutrients diversity and bioactivity of the fermented foods since ancient times. This study aimed at investigating the effects of various fermentation starters on the physicochemical, antioxidant, antimicrobial, and antidiabetic properties of the blueberry juices. The blueberry juices were fermented by natural fermentation (NFBJ), self-made starters fermentation (SFBJ), and commercial starters fermentation (CFBJ), respectively, while fresh blueberry juice (BBJ) was processed without fermentation for comparison. RESULTS: Probiotics-fermented blueberry juices (SFBJ and CFBJ) showed less total and reducing sugars, higher titratable acidity, wider variety and higher amounts of organic acids compared to non-fermented blueberry juice (BBJ). All the fermented blueberry juices (NFBJ, SFBJ, and CFBJ) showed significantly (P < 0.05) higher antioxidant potentials than that of BBJ measured by ABTS, CUPRAC, and FRAP assays. The SFBJ exhibited highest antibacterial activities against the Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium with the inhibition zones of 38.84 ± 1.74, 34.91 ± 1.53, and 36.18 ± 3.16 mm in diameter, respectively. Compared to BBJ, the α-glucosidase inhibitory activity of the SFBJ and CFBJ increased by 2-3 folds. The α-amylase inhibitory activity of the SFBJ and CFBJ has been increased by 600%, while the spontaneous fermentation showed no improvement. The SFBJ and CFBJ promoted glucose consumption of HepG2 cell lines, indicating the promising potential for a higher glucose bio-utilization. CONCLUSIONS: The self-made and commercial starters fermented blueberry juices showed remarkable improvements in the antioxidant, antimicrobial, and antidiabetic activities compared to non-fermented and spontaneous fermented juices, indicating their promising potentials as an antihyperglycemic agent. This article is protected by copyright. All rights reserved.

8.
Cancer Lett ; 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33482262

RESUMO

Tumor metastasis is the main cause of death in patients with triple-negative breast cancer (TNBC). Bone marrow-derived mesenchymal stem cells (BM-MSCs) have tropism towards tumor tissues, and can be converted into tumor-associated mesenchymal stromal cells (TA-MSCs) to facilitate TNBC metastasis through interactions with tumor-associated macrophages (TAMs). However, the underlying molecular mechanisms are complex and unclear, and effective strategies to suppress tumor metastasis via eliminating TA-MSCs are still lacking. Here, we demonstrate that fibroblast activation protein alpha (FAPα) was overexpressed in TA-MSCs, which prompts TA-MSCs to secrete multiple C-C motif chemokine ligands, promoting C-C motif chemokine receptor 2 (CCR2)+ TAM recruitment and facilitating TAM polarization into the M2 phenotype, thereby promoting TNBC pulmonary metastasis. Z-GP-DAVLBH, an FAPα-activated vinblastine prodrug, induces FAPα+ TA-MSC apoptosis, which significantly suppresses CCR2+ TAM recruitment and polarization, thus inhibiting pulmonary metastasis of orthotopic TNBC cell-derived xenografts and patient-derived xenografts. This study provides insight into an important role of FAPα in mediating TA-MSC-induced TNBC metastasis and provides compelling evidence that targeting TA-MSCs with an FAPα-activated prodrug is a promising strategy for suppressing TNBC metastasis.

9.
Arch. bronconeumol. (Ed. impr.) ; 57(1): 51-60, ene. 2021. tab, graf
Artigo em Inglês | IBECS-Express | IBECS | ID: ibc-ET5-2495

RESUMO

INTRODUCTION: The impact of preoperative nutritional status on survival in lung cancer (LC) patients with underlying chronic obstructive pulmonary disease (COPD) is still unclear. We hypothesized that presurgical nutritional assessment may differentially predict mortality in patients with resectable LC with moderate COPD and relatively well-preserved nutritional status. METHODS: Nutritional assessment [body mass index (BMI), blood parameters including albumin and protein levels, and body weight loss], and other clinical parameters [cigarette smoking (CS) history, LC staging and histological subtypes, COPD severity, lung function, and adjuvant therapy] were evaluated in 125 patients from the LC Mar Prospective Cohort: 87 LC-COPD patients and 38 LC patients without COPD before thoracotomy. Ten-year overall survival (OS) was analyzed in all patients. RESULTS: Prior to thoracotomy, in LC-COPD patients compared to LC, BMI and albumin declined relatively, low levels of the parameters BMI, albumin, and total proteins were associated with poorer 10-year survival, especially in the LC-COPD. CS burden also correlated with impaired survival. COPD per se worsened the prognosis in LC patients. CONCLUSIONS: In the present cohort of LC patients with resectable tumors and relatively well-preserved nutritional status, the parameters BMI and blood albumin and protein levels measured prior to thoracotomy predicted OS, especially in those with COPD. These are clinically relevant findings, since values of those nutritional parameters were within the normal ranges in the majority of the analyzed patients. A thorough nutritional preoperative assessment should be included in the study of patients with resectable LC, particularly in those with chronic airway obstruction


INTRODUCCIÓN: El impacto del estado nutricional preoperatorio en la supervivencia en pacientes con cáncer de pulmón (CP) y enfermedad pulmonar obstructiva crónica (EPOC) subyacente aún no está claro. Planteamos la hipótesis de que la evaluación nutricional prequirúrgica puede predecir diferencialmente la mortalidad en pacientes con CP resecable y EPOC moderada, y un estado nutricional relativamente bien conservado. MÉTODOS: Se evaluaron el estado nutricional (índice de masa corporal [IMC], parámetros sanguíneos que incluyeron los niveles de albúmina y proteínas y pérdida de peso corporal) y otros parámetros clínicos (antecedentes de tabaquismo, estadificación del CP y el subtipo histológico, gravedad de la EPOC, función pulmonar y terapia adyuvante) en 125 pacientes de la cohorte prospectiva de CP del Hospital del Mar: 87 pacientes con EPOC-CP y 38 pacientes con CP sin EPOC antes de la toracotomía. Se analizó la supervivencia global (SG) a 10 años en todos los pacientes. RESULTADOS: Antes de la toracotomía, en los pacientes con EPOC-CP, el IMC y la albúmina disminuyeron en comparación con los del grupo de CP; los niveles bajos de los parámetros IMC, albúmina y proteínas totales se asociaron con menor supervivencia a 10 años, especialmente en los EPOC-CP. La carga tabáquica también se correlacionó con una disminución en la supervivencia. La EPOC empeoró per se el pronóstico en pacientes con CP. CONCLUSIONES: En la presente cohorte de pacientes con CP resecable y estado nutricional relativamente bien conservado, el IMC y los niveles de albúmina y proteínas en sangre medidos antes de la toracotomía predijeron la SG, especialmente en aquellos con EPOC. Estos son hallazgos clínicamente relevantes, ya que los valores de esos parámetros nutricionales estaban dentro de los rangos normales en la mayoría de los pacientes analizados. Se debe incluir una evaluación nutricional preoperatoria exhaustiva en el estudio de pacientes con CP resecable, particularmente en aquellos con obstrucción pulmonar crónica

10.
Appl Opt ; 60(1): 125-134, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33362080

RESUMO

Imaging the high-precision magnetic distribution generated by the surface current of chips and chip-like structures is an important way to measure thermal parameters of core components. Based on a high-concentration nitrogen-vacancy color center ensemble in diamond, the imaging magnetic field distribution is performed in a wide-field microscope. The magnetic vector detection and reduction model is verified first with continuous wave optical detection of magnetic resonance technology. By systematically measuring the distribution of the electromagnetic field generated on the surface of the micro-wire under different microwave power and different laser power conditions, the imaging quality of the wide-field imaging system can be optimized by adjusting the experimental parameters. Then, the electromagnetic field distribution imaging on the wire surface under different current intensities is obtained. In this way, accurate measurement and characterization of the magnetic distribution on the surface of the micro-wire is realized. Finally, at the field of view in the range of 480µm×270µm, the magnetic intensity is an accurate characterization in 0.5-10 Gs, and the magnetic detection sensitivity can be increased from 100 to 20µT/Hz1/2. The results show the accurate magnetic distribution imaging for chips and chip-like structures, which provide a new method for chip function detection and fault diagnosis based on precision quantum measurement technology.

11.
Cell Discov ; 6(1): 89, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33298886

RESUMO

Definitive hematopoiesis generates hematopoietic stem/progenitor cells (HSPCs) that give rise to all mature blood and immune cells, but remains poorly defined in human. Here, we resolve human hematopoietic populations at the earliest hematopoiesis stage by single-cell RNA-seq. We characterize the distinct molecular profiling between early primitive and definitive hematopoiesis in both human embryonic stem cell (hESC) differentiation and early embryonic development. We identify CD44 to specifically discriminate definitive hematopoiesis and generate definitive HSPCs from hESCs. The multipotency of hESCs-derived HSPCs for various blood and immune cells is validated by single-cell clonal assay. Strikingly, these hESCs-derived HSPCs give rise to blood and lymphoid lineages in vivo. Lastly, we characterize gene-expression dynamics in definitive and primitive hematopoiesis and reveal an unreported role of ROCK-inhibition in enhancing human definitive hematopoiesis. Our study provides a prospect for understanding human early hematopoiesis and a firm basis for generating blood and immune cells for clinical purposes.

12.
Phys Chem Chem Phys ; 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33346754

RESUMO

The effect of atomic hydrogen adsorption on AnO2 (An = Th, U, and Pu) surfaces is studied in the framework of density functional theory and Hubbard-corrected density functional theory. Several adsorption coverages (1/3, 1/2, 2/3, and 1 monolayer) are considered. For the band insulator ThO2, surface metallicity induced by hydrogen adsorption is observed due to the electron donation of the hydrogen to the surface. But this effect is found to be strongly suppressed by electronic correlation for the Mott insulators UO2 and PuO2 because the electrons from the adsorbed hydrogen atoms occupy the localized 5f orbitals of the surface U/Pu atoms.

13.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 37(6): 1031-1036, 2020 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-33369342

RESUMO

To investigate the computed tomography (CT) characteristics and differential diagnosis of high altitude pulmonary edema (HAPE) and COVID-19, CT findings of 52 cases of HAPE confirmed in Medical Station of Sanshili Barracks, PLA 950 Hospital from May 1, 2020 to May 30, 2020 were collected retrospectively. The size, number, location, distribution, density and morphology of the pulmonary lesions of these CT data were analyzed and compared with some already existed COVID-19 CT images which come from two files, "Radiological diagnosis of COVID-19: expert recommendation from the Chinese Society of Radiology (First edition)" and "A rapid advice guideline for the diagnosis and treatment of 2019 novel corona-virus (2019-nCoV) infected pneumonia (standard version)". The simple or multiple ground-glass opacity (GGO) lesions are located both in the HAPE and COVID-19 at the early stage, but only the thickening of interlobular septa, called "crazy paving pattern" belongs to COVID-19. At the next period, some increased cloudy shadows are located in HAPE, while lesions of COVID-19 are more likely to develop parallel to the direction of the pleura, and some of the lesions show the bronchial inflation. At the most serious stage, both the shadows in HAPE and COVID-19 become white, but the lesions of HAPE in the right lung are more serious than that of left lung. In summary, some cloudy shadows are the feature of HAPE CT image, and "crazy paving pattern" and "pleural parallel sign" belong to the COVID-19 CT, which can be used for differential diagnosis.


Assuntos
Altitude , Edema Pulmonar/diagnóstico por imagem , China , Diagnóstico Diferencial , Humanos , Pulmão/diagnóstico por imagem , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
14.
Rev Sci Instrum ; 91(12): 125110, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33379976

RESUMO

Navigation in primates is generally supported by cognitive maps. Such a map endows an animal with navigational planning capabilities. Numerous methods have been proposed to mimic these natural navigation capabilities in artificial systems. Based on self-navigation and learning strategies in animals, we propose in this work a place recognition strategy for brain-inspired navigation. First, a place recognition algorithm structure based on convolutional neural networks (CNNs) is introduced, which can be applied in the field of intelligent navigation. Second, sufficient images are captured at each landmark and then stored as a reference image library. Simple linear iterative clustering (SLIC) is used to segment each image into superpixels with multi-scale viewpoint-invariant landmarks. Third, highly representative appearance-independent features are extracted from these landmarks through CNNs. In addition, spatial pyramid pooling (SPP) layers are introduced to generate a fixed-length CNN representation, regardless of the image size. This representation boosts the quality of the extracted landmark features. The proposed SLIC-SPP-CNN place recognition algorithm is evaluated on one collected dataset and two public datasets with viewpoint and appearance variations.

15.
Artigo em Inglês | MEDLINE | ID: mdl-33381887

RESUMO

The reversibility of metal anode is a fundamental challenge to the lifetime of rechargeable batteries. Though being widely employed in aqueous energy storage systems, metallic zinc suffers from dendrite formation that severely hinders its applications. Here we report texturing Zn as an effective way to address the issue of zinc dendrite. An in-plane oriented Zn texture with preferentially exposed (002) basal plane is demonstrated via a sulfonate anion-induced electrodeposition, noting no solid report on (002) textured Zn till now. Anion-induced reconstruction of zinc coordination is revealed to be responsible for the texture formation. Benchmarking against its (101) textured-counterpart by the conventional sulphate-based electrolyte, the Zn (002) texture enables highly reversible stripping/plating at a high current density of 10 mA cm -2 , showing its dendrite-free characteristics. The Zn (002) texture-based aqueous zinc battery exhibits excellent cycling stability. The developed anion texturing approach provides a pathway towards exploring zinc chemistry and prospering aqueous rechargeable batteries.

16.
Med Sci Monit ; 26: e927674, 2020 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-33342993

RESUMO

BACKGROUND The aim of this study was to analyze the clinical features and laboratory indices of patients with coronavirus disease (COVID-19) and explore their association with the severity of the disease. MATERIAL AND METHODS A total of 61 patients with COVID-19 were divided into groups with common symptoms and with severe diseases, and clinical data were collected to analyze and compare the differences between them. RESULTS In patients with severe COVID-19, compared with the common group, lymphocyte count and albumin levels were lower, and aspartate aminotransferase (AST), blood urea, blood creatinine, lactate dehydrogenase (LDH), and C-reactive protein (CRP) levels, and prothrombin time (PT) were elevated (all P<0.05). The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), mean platelet volume-to-lymphocyte ratio (MPVLR), and C-reactive protein-to-albumin ratio (CAR) were significantly elevated in the severe group compared with the group with common symptoms; however, the lymphocyte-to-monocyte ratio (LMR) was significantly reduced (P<0.05). Univariate logistic regression showed that lower lymphocyte count, prolonged PT, elevated CRP and LDH levels, and elevated NLR, PLR, MPVLR, and CAR were risk factors for COVID-19 severity (P<0.05). Multivariate logistic regression showed that elevated CRP levels (odds ratio [OR], 0.028; 95% confidence interval [CI]: 0.002-0.526; P=0.017), prolonged PT (OR, 0.014; 95% CI: 0.001-0.341; P=0.09), and an MPVLR >8.9 (OR, 0.026; 95% CI: 0.002-0.349; P=0.006) were independent risk factors for COVID-19 severity. CONCLUSIONS Elevated CRP and prolonged PT, and an MPVLR >8.9 were independent risk factors for COVID-19 severity.


Assuntos
/epidemiologia , Infecções por Coronavirus/diagnóstico , Adulto , Aspartato Aminotransferases/sangue , Plaquetas , Proteína C-Reativa/análise , China/epidemiologia , Coronavirus/patogenicidade , Infecções por Coronavirus/sangue , Creatinina/análise , Feminino , Humanos , Pacientes Internados , L-Lactato Desidrogenase/sangue , Contagem de Linfócitos , Linfócitos/química , Masculino , Volume Plaquetário Médio , Pessoa de Meia-Idade , Monócitos , Neutrófilos/química , Estudos Retrospectivos , Albumina Sérica/análise , Índice de Gravidade de Doença
17.
JAMA ; 2020 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-33300950

RESUMO

Importance: Among all subtypes of breast cancer, triple-negative breast cancer has a relatively high relapse rate and poor outcome after standard treatment. Effective strategies to reduce the risk of relapse and death are needed. Objective: To evaluate the efficacy and adverse effects of low-dose capecitabine maintenance after standard adjuvant chemotherapy in early-stage triple-negative breast cancer. Design, Setting, and Participants: Randomized clinical trial conducted at 13 academic centers and clinical sites in China from April 2010 to December 2016 and final date of follow-up was April 30, 2020. Patients (n = 443) had early-stage triple-negative breast cancer and had completed standard adjuvant chemotherapy. Interventions: Eligible patients were randomized 1:1 to receive capecitabine (n = 222) at a dose of 650 mg/m2 twice a day by mouth for 1 year without interruption or to observation (n = 221) after completion of standard adjuvant chemotherapy. Main Outcomes and Measures: The primary end point was disease-free survival. Secondary end points included distant disease-free survival, overall survival, locoregional recurrence-free survival, and adverse events. Results: Among 443 women who were randomized, 434 were included in the full analysis set (mean [SD] age, 46 [9.9] years; T1/T2 stage, 93.1%; node-negative, 61.8%) (98.0% completed the trial). After a median follow-up of 61 months (interquartile range, 44-82), 94 events were observed, including 38 events (37 recurrences and 32 deaths) in the capecitabine group and 56 events (56 recurrences and 40 deaths) in the observation group. The estimated 5-year disease-free survival was 82.8% in the capecitabine group and 73.0% in the observation group (hazard ratio [HR] for risk of recurrence or death, 0.64 [95% CI, 0.42-0.95]; P = .03). In the capecitabine group vs the observation group, the estimated 5-year distant disease-free survival was 85.8% vs 75.8% (HR for risk of distant metastasis or death, 0.60 [95% CI, 0.38-0.92]; P = .02), the estimated 5-year overall survival was 85.5% vs 81.3% (HR for risk of death, 0.75 [95% CI, 0.47-1.19]; P = .22), and the estimated 5-year locoregional recurrence-free survival was 85.0% vs 80.8% (HR for risk of locoregional recurrence or death, 0.72 [95% CI, 0.46-1.13]; P = .15). The most common capecitabine-related adverse event was hand-foot syndrome (45.2%), with 7.7% of patients experiencing a grade 3 event. Conclusions and Relevance: Among women with early-stage triple-negative breast cancer who received standard adjuvant treatment, low-dose capecitabine maintenance therapy for 1 year, compared with observation, resulted in significantly improved 5-year disease-free survival. Trial Registration: ClinicalTrials.gov Identifier: NCT01112826.

18.
Phys Rev Lett ; 125(21): 210401, 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33275014

RESUMO

When an observable is measured on an evolving coherent quantum system twice, the first measurement generally alters the statistics of the second one, which is known as measurement backaction. We introduce, and push to its theoretical and experimental limits, a novel method of backaction evasion, whereby entangled collective measurements are performed on several copies of the system. This method is inspired by a similar idea designed for the problem of measuring quantum work [Perarnau-Llobet et al., Phys. Rev. Lett. 118, 070601 (2017)PRLTAO0031-900710.1103/PhysRevLett.118.070601]. By using entanglement as a resource, we show that the backaction can be extremely suppressed compared to all previous schemes. Importantly, the backaction can be eliminated in highly coherent processes.

19.
J Hosp Infect ; 2020 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-33290814

RESUMO

BACKGROUND: The nosocomial infection of Klebsiella pneumoniae (Kp) and drug resistance of Kp among neonates is a major concern. Hypervirulent Klebsiella pneumoniae (hvKp) infections are gradually increasing worldwide. Carbapenem-resistant hvKp infection has brought challenges to clinical treatment. AIM: To evaluate the changes in drug resistance trends of Kp strains in neonatal intensive care unit (NICU) nosocomial infections, analyze drug resistance genes and virulence genes of carbapenem-resistant klebsiella pneumoniae (CRKP) and identify whether these CRKP strains are hvKp. METHODS: A total of 80 neonates with Kp nosocomial infections from 2013 to 2018 were retrospectively studied. Drug susceptibility testing was performed on 80 Kp strains, among which the 12 CRKP strains were further studied. FINDINGS: Kp accounted for 26.94% of nosocomial infections in the NICU. CRKP strains accounted for 15.00%. Among the 80 nosocomial infection Kp strains, CRKP strains accounted for 33.3% and 53.3% in 2017 and 2018 respectively. One of the 12 CRKP strains was positive in the drawing test. The 12 CRKP strains were divided into 4 complete genome sequence types: cgST1(n=2), cgST2(n=1), cgST3(n=1) and cgST4(n=8). Among genes that mediated carbapenem resistance, strains of cgST4 carried NDM-5, strains of cgST2 and cgST3 carried NDM-1, and strains of cgST1 carried IMP-4. None of the 12 CRKP strains carried rmpA/rmpA2 (highly related with hvKp). CONCLUSIONS: Nosocomial infection of CRKP among neonates are becoming common, but no hvKp was found among the CRKP strains in this study.

20.
PeerJ ; 8: e10225, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33173620

RESUMO

Purpose: Recent studies have determined that long non-coding RNAs (lncRNAs) are potential prognostic biomarkers for non-small cell lung cancers (NSCLCs). The purpose of this study was to analyze the function and associated pathways of zinc finger protein multitype 2 antisense RNA 1 (ZFPM2-AS1) in NSCLC cells. Methods: We used qRT-PCR to analyze ZFPM2-AS1's transcription level. Its proliferation, migration, and invasion capacities were determined using MTT, colony forming, wound healing, and transwell assays. We additionally analyzed the correlation between ZFPM2 and immune infiltration using the Tumor Immune Estimation Resource (TIMER) database, and the protein expression levels using Western blots. Results: We found that ZFPM2-AS1 expression in NSCLC specimens and cell lines was elevated compared to the control group. ZFPM2-AS1 is an oncogene and independent prognostic predictor of poor survival in NSCLCs, and its expression had a positive correlation with tumor size and lymph node metastasis in our clinical data. MTT, colony forming, wound healing, and transwell assays showed a positive correlation between ZFPM2-AS1 expression and the proliferation, migration, and invasion of NSCLC cells in the presence and absence of interferon- (IFN-γ). Using the TIMER database, we hypothesized that ZFPM2 was negatively correlated with ZFPM2-AS1 expression, as well as the immune infiltration levels in lung adenocarcinoma (LUAD). Finally, we found that ZFPM2-AS1 negatively regulated ZFPM2 expression, and had a positive correlation with PD-L1 expression through the JAK-STAT and AKT pathways. Conclusion: Our study confirmed that ZFPM2-AS1 promotes the proliferation, migration, and invasion of NSCLC cells via the JAK-STAT and AKT pathways. Further research on the ZFPM2-AS1 pathway regulation mechanism is needed.

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