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1.
Clin Chim Acta ; 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36096209

RESUMO

BACKGROUND: Medium-chain acyl-coenzyme A dehydrogenase deficiency (MCADD) is a rare inherited metabolic disorder of fatty acid ß-oxidation and one of the most common inborn errors of metabolism. The incidence of MCADD varies among regions and ethnic groups. To date, few cases of MCADD have been documented in China. OBJECTIVE: The present study aimed to find out the novel genetic pathogenic variants in the Chinese patients and evaluate the detection rate of the disease of high-frequency ACADM pathogenic variants in different regions of China. METHODS: 6 cases of MCADD were screened by tandem mass spectrometric (MS/MS) among 245 054 newborns. We performed next-generation sequencing on 6 families of infants with MCADD. We used the REVEL method to predict the protein function of the detected missense variants and used SPDBV 4.10 to predict the protein 3D structure model. We identified pathogenic variants of ACADM gene in 6 cases of MCADD, and then assessed these variants through Sanger sequencing and association analysis. RESULTS: The incidence of neonatal MCADD was 1/40,842 in Henan province. Among the 6 patients, five cases were compound heterozygous variants, one case was homozygous variants. DNA sequencing revealed 4 known (c.449_452del, c.1085G>A, c.1229T>C, c.589A>G) and 3 novel mutations (c.849+5_849+8del, c.427A>G, c.1181C>T) in the ACADM gene. Mutation c.1085G>A (p.G362E) was most frequent among Henan people and shows obvious differences between North and South of China. CONCLUSION: MCADD is relatively rare in China, and c.1085G>A (p.G362E) is a common mutation in Henan population. Our findings, especially novel variants, will help improve the understanding of the genetic background and have facilitated clinical diagnosis and genetic counseling for the affected families.

2.
Sci Rep ; 12(1): 15695, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36127497

RESUMO

To evaluate the effect of SGLT2 inhibitor (SGLT2i) on albuminuria, nephrin (NPH) and transforming-growth-factor-beta1 (TGF-ß1) levels in urine and low-grade inflammation in type 2 diabetes (T2D) patients. A randomized, blank-controlled clinical trial included 68 T2D patients and 10 controls. Based on the urinary albumin-to-creatinine ratio (UACR), 68 diabetic patients were stratified into three levels, UACR < 30 mg/g, UACR ≧ 30 mg/g to ≦ 300 mg/g and UACR ˃ 300 mg/g, who were randomized (1:1:1) to receive SGLT2i treatment for 12 weeks. The concentrations of NPH and TGF-ß1 in urine were measured as indications of podocyte injury and renal fibrosis. Low-grade inflammation was assessed by the levels of IL-6, TNFα and hsCRP. After 12 weeks of SGLT2i treatment, the levels of UACR and NPH decreased, UTGF-ß1 increased in the T2D with microalbuminuria and macroalbuminuria groups, NPH (1.12 [0.59, 1.29] vs. 0.71 [0.41, 1.07] µg/ml, P = 0.022) and (1.29 [0.99, 1.96] vs. 0.93 [0.57, 1.31] µg/ml, P = 0.002), UTGF-ß1 (4.88 ± 1.31 vs. 7.27 ± 1.21 pg/ml, P  < 0.001) and (4.30 ± 1.34 vs. 6.78 ± 2.59 pg/ml, P  < 0.001), respectively. The changes in NPH were positively correlated with the UACR and negatively correlated with UTGF-ß1 in T2D with albuminuria. SGLT2i alleviate nephrin loss and enhance TGF-ß1 excretion in urine in T2DM with albuminuria. The anti-albuminuric effect of SGLT2i could be attributed to mitigating podocyte apoptosis and attenuating renal fibrosis.Trial registration This clinical trial was registered on 15/10/2019, in ClinicalTrials.gov, and the registry number is NCT04127084.


Assuntos
Diabetes Mellitus Tipo 2 , Nefropatias , Inibidores do Transportador 2 de Sódio-Glicose , Albuminúria/tratamento farmacológico , Albuminúria/urina , Proteína C-Reativa , Creatinina/urina , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/urina , Fibrose , Humanos , Inflamação/tratamento farmacológico , Interleucina-6/uso terapêutico , Nefropatias/tratamento farmacológico , Proteínas de Membrana , Inibidores do Transportador 2 de Sódio-Glicose/farmacologia , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico , Fator de Crescimento Transformador beta1/uso terapêutico , Fator de Necrose Tumoral alfa/uso terapêutico
3.
Womens Health (Lond) ; 18: 17455057221122597, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36129002

RESUMO

Here, we reported a case of a 16-year-old Chinese female patient (46, XX) diagnosed as 17α-hydroxylase/17, 20-lyase deficiency (17-OHD) in June 2018 and over 3 years follow-up outcomes; 17-OHD is a rare form of congenital adrenal hyperplasia. The patient presented with primary amenorrhea, underdeveloped secondary sexual characteristics, hypertension and hypokalemia. Hormonal findings revealed decreased estrogen and androgen, increased progesterone, low cortisol concentration and compensatory high adrenocorticotropic hormone level. Mutation analysis of the CYP17A1 gene identified the c.1459_1467del GACTCTTTC homozygous deletion in exon 8, namely, D487_F489del mutation, resulting in the deletion of Aspartate-Serine-Phenylalanine amino acids. The patient's father and mother were all heterozygous carriers of this mutation. The diagnosis and follow-up outcomes provided useful insights to support clinical decision-making and appropriate treatment.


Assuntos
Liases , Esteroide 17-alfa-Hidroxilase , Adolescente , Hormônio Adrenocorticotrópico/genética , Androgênios , Ácido Aspártico/genética , Estrogênios , Feminino , Seguimentos , Homozigoto , Humanos , Hidrocortisona , Liases/genética , Oxigenases de Função Mista/genética , Fenilalanina/genética , Progesterona , Deleção de Sequência , Serina/genética , Esteroide 17-alfa-Hidroxilase/genética , Esteroide 17-alfa-Hidroxilase/metabolismo
4.
Food Chem Toxicol ; 169: 113433, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36122811

RESUMO

Cyclophosphamide (CTX) is a widely used anticancer drug that can cause liver injury, but there is no effective treatment available at present. The antioxidant properties of Lactobacillus plantarum Lp2 in vitro and its effect on CTX-induced liver injury in mice were investigated thoroughly. The order of antioxidant capacity of the fermentate of Lp2 was as followed: fermented supernatant > cell-free extract > intact cell. BALB/c mice were intraperitoneally injected with 80 mg/kg BW/d CTX for 3 days to build a liver injury model, then treated with Lp2 fermented supernatant (Lp2-s) and Lp2 culture broth (Lp2). After 10 days, the indicators of oxidative stress and liver injury were measured. Both Lp2-s and Lp2 restored the levels of T-SOD, CAT, GSH-Px, MDA, GSH, ALT, and AST. The western blotting results showed that Lp2-s and Lp2 ameliorated CTX-induced oxidative damage and hepatocyte apoptosis via inhibiting MAPKs pathway and strengthening Nrf2/HO-1/NQO1 antioxidant defense system, thus inhibiting the mitochondrial-mediated apoptosis pathway. Therefore, both Lp2-s and Lp2 had similar protective effects on CTX-induced liver injury.

5.
Front Nutr ; 9: 981533, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36046129

RESUMO

Background: Sarcopenia is associated with poor clinical outcomes in patients with locally advanced gastric cancer (LAGC). Currently, the diagnostic criteria for sarcopenia are complex and laborious. Increased evidence suggests the inflammatory state of the body is closely associated with the development of sarcopenia. The systemic immune-inflammatory index (SII) and the prognostic nutritional index (PNI) are representative blood indicators of the status of the systemic inflammatory response, but the clinical significance of the combined testing of these two indicators remains unclear. We aimed to develop a simple and practical risk score (SII-PNI score) to screen patients with LAGC for sarcopenia on admission for early diagnosis. Methods: We registered a prospective clinical study from January 2011 to May 2016 involving 134 patients with LAGC undergoing radical surgical resection. All patients followed the definition of sarcopenia in the Asian Working Group on Sarcopenia (AWGS) guidelines and were divided into sarcopenia and non-sarcopenia groups. SII-PNI score 0-2 was scored as 2 for high SII (≥432.9) and low PNI ( ≤ 49.5); score 1, either high SII or low PNI; score 0, no high SII or low PNI. Results: All patients underwent radical surgery, including 31 patients (23.13%) with sarcopenia according to AWGS criteria. The SII-PNI score was significantly lower in the non-sarcopenic patients than in the sarcopenic patients (p < 0.001). Logistic multivariate analysis showed that the SII-PNI score predicted an independent prognostic factor for sarcopenia (p < 0.001). Patients with high SII-PNI scores had significantly worse prognosis than those with low SII-PNI scores (p < 0.001). The SII-PNI score was an independent prognostic factor for predicting overall survival and disease-free survival (p = 0.016, 0.023). Conclusion: Peripheral blood parameters SII-PNI scores accurately identify sarcopenia in patients with LAGC and could be used as potential systemic markers.

6.
Medicine (Baltimore) ; 101(36): e30151, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36086680

RESUMO

PURPOSE: To elucidate the relationship between peripheral edema and programmed cell death-1/programmed cell death ligand 1 (PD-1/PD-L1) inhibitors, the meta-analysis was performed. METHOD: Following the guidelines of Preferred Reporting Items for Systematic Reviews and Meta-analyses, all-grade and grade 3-5 of peripheral edema data extracted from clinical trials were taken into account for the final comprehensive assessments. RESULTS: Twenty-seven PD-1/PD-L1-related clinical trials with peripheral edema data were collected. Compared with chemotherapy (PD-1/PD-L1 vs chemotherapy), the risk of developing peripheral edema for all-grade was much lower (odds ratio [OR] = 0.36, 95% confidence interval [CI]: [0.23, 0.56], Z = 4.55 [P < .00001]). When PD-1/PD-L1 plus chemotherapy were compared with chemotherapy, no significant analysis results for all-grade was found (OR = 1.15, 95% CI:[0.93, 1.44], I2 = 25%, Z = 1.27 [P = .20]). Similar risk trends could also be found when the incidence risk of peripheral edema for grade 3-5 was evaluated. No obvious publication bias was identified throughout the total analysis process. CONCLUSION: The effect of PD-1/PD-L1 inhibitor on the risk of developing peripheral edema was weaker than that of chemotherapy, and the combination with chemotherapy slightly increased the incidence risk of developing peripheral edema without statistical significance.


Assuntos
Antígeno B7-H1 , Neoplasias , Antígeno B7-H1/uso terapêutico , Edema/tratamento farmacológico , Humanos , Inibidores de Checkpoint Imunológico/efeitos adversos , Incidência , Neoplasias/tratamento farmacológico , Receptor de Morte Celular Programada 1/uso terapêutico
7.
Carbohydr Polym ; 296: 119923, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36087977

RESUMO

A reliable electrophysiological electrode interface (EEI) between bioelectronic devices and biological tissues is indispensable to achieve the high fidelity recording of bioelectricity. However, there is an inherent tradeoff among EEI's electrochemical characteristics, mechanical properties and biocompatibility when considering the desired nanostructure and optimum composition. Here, we proposed a mechanically matched, highly conductive and biocompatible EEI, a tissue-like metal-doped hydrogel which could enable excellent electro-biosensing, by bringing disulfide modified silver nanowires into difunctional hyaluronan/carboxymethyl chitosan composite. The intensity of cortical signals at specific frequency domain recorded by the hydrogel-based EEI is doubled, which is significant for the diagnosis of epilepsy. Furthermore, the natural gel matrix could lead to seamless bio-integration between EEI and the tissue site of interest, minimizing signal dissipation without causing obvious inflammatory response. Overall, the EEI we designed contributes to improving tissue-device integration as well as bioelectronic device's performance and further leads to more effective human-computer interfaces.


Assuntos
Hidrogéis , Nanofios , Eletrodos , Humanos , Hidrogéis/química , Polissacarídeos , Prata
8.
Front Oncol ; 12: 927781, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091139

RESUMO

Background: Neoadjuvant chemotherapies have been widely recommended in patients with locally advanced gastric cancer (LAGC). However, the evidence of combining neoadjuvant chemotherapy with anti-programmed death 1 (anti-PD-1) antibody therapy for patients with LAGC is lacking. Thus, we conducted a single-arm phase II trial to evaluate the efficacy and safety of the anti-PD-1 antibody sintilimab plus XELOX regimen (capecitabine plus oxaliplatin) in patients with LAGC. Methods: Patients with LAGC (cT3-4 N+ M0, CY0, P0) were enrolled and received four preoperative cycles of sintilimab (200 mg, IV, Q21d) plus XELOX (oxaliplatin 130 mg/m2, IV, d1 with capecitabine 1,000 mg/m2, bid, d1-d14, Q21d) therapy. The primary endpoint was the pathological complete response (pCR) rate. This clinical trial was registered at Chictr.org.cn (trial number: ChiCTR2000030414). Results: Thirty patients were enrolled from March 2020 to July 2021, with a median age of 62 years (range, 30-72), and 18 (60.0%) were men. There were 19 (63.3%) patients with PD-L1 CPS ≥1.The pCR rate was 33.3% [95% confidence interval (CI), 17.3%-52.8%], and the major pathologic response (MPR) rate was 63.3% (95% CI, 43.9%-80.1%). All the patients underwent R0 resection. The objective response rate (ORR) and the disease control rate (DCR) were 70.0% (95% CI, 50.6%-85.3%) and 100% (95% CI, 88.4%-100%), respectively. Downstaging of the overall TNM stage was observed in 22 (73.3%) patients. The pCR rate in patients with PD-L1 CPS ≥1 and patients with PD-L1 CPS <1 was 42.1% vs. 18.2% (P = 0.246), whereas the MPR rate was 78.9% vs. 36.4% (P = 0.047). The potential immune-related adverse events (irAEs) were hypothyroidism (3.3%), pneumonia (10.0%), and dermatitis (6.7%). Grade3 common treatment-related adverse events (TRAEs) were ALT increase (3.3%), AST increase (3.3%), and dermatitis (3.3%) during the neoadjuvant therapy. There were no severe complications or death related to the surgery. Conclusion: Sintilimab plus XELOX as neoadjuvant therapy showed an encouraging pCR rate, MPR rate, and manageable safety. This combination of regimens might provide a new option for patients with LAGC. Clinical Trial Registration: Chictr.org.cn, identifier ChiCTR2000030414.

9.
Anal Bioanal Chem ; 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36100705

RESUMO

In this paper, a surface-enhanced Raman scattering (SERS) strategy was constructed for the determination of antihypertensive drugs irbesartan (IRB) and doxazosin mesylate (DOX). ß-Cyclodextrin-capped silver nanoparticles (CD-AgNPs) are employed as SERS-active substrate. The introduction of ß-CD with hydrophobic cavity can capture drug molecules to form host-guest complex, making the drug molecules closer to the electromagnetic enhancement field of the AgNPs, thereby enhancing the SERS signal of drug molecules with low Raman cross-section. The vibrational modes of IRB and DOX are assigned by density functional theory calculations. The linear response from 3.0 × 10-7 to 1.0 × 10-5 mol L-1 for IRB and 3.0 × 10-7 to 2.0 × 10-5 mol L-1 for DOX and low limits of detection (LOD) 7.5 × 10-8 mol L-1 for IRB and 8.6 × 10-8 mol L-1 for DOX can be achieved. Meanwhile, this SERS approach can be applicable to determine IRB and DOX in commercial drug tablets, healthcare products, and human urine samples with recoveries of 90.8-115.7% and 90.0-113.5%, respectively, with relative standard deviation (RSD) less than 4.5%. This designed SERS strategy enables for the rapid determination of IRB and DOX in drug quality monitoring and illegal additives in healthcare products as well as clinical applications.

10.
J Oncol ; 2022: 1911592, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36059806

RESUMO

Pancreatic cancer (PC) has a high degree of malignancy and poor prognosis, and countless patients have distant metastasis when diagnosed. Gemcitabine (GEM) chemotherapy is one of the main ways of treatment. However, PC cells have been displayed chemoresistance to GEM during treatment. Circular RNAs (circRNAs) have been demonstrated to be the most popular diagnostic and prognostic biomarkers in PC with GEM resistance. Here, we assessed the potential of circLMTK2 in the GEM resistance of PC cells. Functional assays were implemented to measure the impacts of circLMTK2 on the proliferation, migration/invasion, and apoptosis of GEM-resistant PC cells. Bioinformatics analysis and mechanical experiments displayed the underlying mechanism of circLMTK2 in GEM-resistant PC cells. We found that circLMTK2 was upregulated in PC and GEM-resistant PC tissues and cells. CircLMTK2 knockdown suppressed proliferation, invasion, migration, and enhanced apoptosis in GEM-resistant PC cells. Moreover, circLMTK2 silencing could decrease GEM resistance-associated tumor size in vivo. In terms of mechanism, circLMTK2 served as a sponge for miR-485-5p, and miR-485-5p bound to p21 (RAC1) activated kinase 1 (PAK1), which were clarified via the dual-luciferase assay in PC cell lines. We confirmed that circLMTK2 knockdown attenuated GEM-resistant PC cells by regulating PAK1 via miR-485-5p. Our study demonstrated that circLMTK2 may be a novel diagnostic and prognostic biomarker in GEM-resistant PC cells.

11.
Nat Commun ; 13(1): 5157, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36055985

RESUMO

Intermetallic compounds formed from non-precious transition metals are promising cost-effective and robust catalysts for electrochemical hydrogen production. However, the development of monolithic nanoporous intermetallics, with ample active sites and sufficient electrocatalytic activity, remains a challenge. Here we report the fabrication of nanoporous Co7Mo6 and Fe7Mo6 intermetallic compounds via liquid metal dealloying. Along with the development of three-dimensional bicontinuous open porosity, high-temperature dealloying overcomes the kinetic energy barrier, enabling the direct formation of chemically ordered intermetallic phases. Unprecedented small characteristic lengths are observed for the nanoporous intermetallic compounds, resulting from an intermetallic effect whereby the chemical ordering during nanopore formation lowers surface diffusivity and significantly suppresses the thermal coarsening of dealloyed nanostructure. The resulting ultrafine nanoporous Co7Mo6 exhibits high catalytic activity and durability in electrochemical hydrogen evolution reactions. This study sheds light on the previously unexplored intermetallic effect in dealloying and facilitates the development of advanced intermetallic catalysts for energy applications.

12.
J Clin Ultrasound ; 50(7): 873-884, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36069462

RESUMO

BACKGROUND: Right ventricular (RV) myocardial work (RVMW) is the latest method used to assess RV function. To date, correlations among RVMW indices and RV systolic and diastolic functions have not been studied. METHODS: A total of 106 healthy volunteers (median age, 34 years; 46% male) were prospectively enrolled. RVMW indices were measured using the RV pressure-strain loop using specific software. The correlations among RVMW indices and other RV functions were analyzed. RESULTS: During the multivariate analysis, the RV global work index (RVGWI) was significantly correlated with RV global longitudinal strain (RV GLS) (p < .0001), pulmonary systolic artery pressure (PASP) (p < .0001), and tricuspid annular (TA) plane systolic excursion (TAPSE) (p = .036). RV global constructive work (RVGCW) was correlated with RV GLS (p < .0001) and PASP (p < .0001). RV global wasted work (RVGWW) was correlated with RV GLS (p = .008) and TA isovolumetric acceleration (TA IVA) (p = .008). RV global work efficiency (RVGWE) was correlated with RV GLS (p < .0001) and tissue Doppler (TD) RV myocardial performance index (TD RMPI) (p = .043). CONCLUSION: RVMW indices showed good correlations with RV myocardial systolic function.


Assuntos
Disfunção Ventricular Direita , Adulto , Diástole , Feminino , Humanos , Masculino , Valor Preditivo dos Testes , Sístole , Disfunção Ventricular Direita/diagnóstico por imagem , Função Ventricular Direita
13.
Int J Food Microbiol ; 381: 109890, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36095867

RESUMO

Blueberry (Vaccinium spp.) is one of the five major healthy foods for humans and is recognized as the "king of the world fruit", which has attracted great interest in the phytogenic prebiotics market. Blueberry fruit is favored for its delicious taste and its various functional ingredients (organic acids, phenolics, minerals and vitamins) with multitherapeutic value (antioxidant, anti-inflammatory, anticancer, neuroprotective and vision improvement properties). However, fresh blueberries are highly perishable since they are vulnerable to mechanical damage and microbial decay, resulting in a short shelf life and inevitable subsequent economic losses. Due to the strong seasonal availability and limited storage period of blueberries, their derived bioactive products have emerged as functional foods. Novel food developments that are currently available include blueberry fruit juice, wine, vinegar, jam, dried fruit, pulp powder, colorant and flavoring additives used in cake, biscuit, bread, yogurt, and jelly. This review systematically describes the current status of blueberry fruit as bioactive ingredients and valuable food products with greater nutraceutical health potential of blueberries.

14.
F S Sci ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36089208

RESUMO

OBJECTIVE: To systematically analyze the cell composition and transcriptome of primary and transformed human endometrial stromal cells (HESCs and THESCs). DESIGN: The primary HESCs from three different donors and one immortalized THESC were collected from the human endometrium at mid-secretory phase and cultured in vitro. SETTING: Academic Research Laboratory PATIENTS: None INTERVENTIONS: None MAIN OUTCOME MEASURES: Single cell RNA-Seq analysis RESULTS: We found the individual differences among the primary HESCs and bigger changes between the primary HESCs and THESCs. Cell clustering with or without integration identified cell clusters belonging to mature, proliferative, and active fibroblasts that were conserved across all the samples at different stages of cell cycles with intensive cell communication signals. All the primary and transformed HESCs can be correlated with some subpopulations of fibroblasts in the human endometrium. CONCLUSIONS: Our study indicated that the primary HESCs and THESCs displayed conserved cell characters and distinct cell clusters. Mature, proliferative and active fibroblasts at different stages or cell cycles were detected across all the samples and presented with complex cell communication network. The cultured HESCs and THESCs retained the features of some subpopulations within the human endometrium.

15.
Front Nutr ; 9: 963265, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118766

RESUMO

Background: It is well known that sarcopenia is a common risk factor in patients with gastrointestinal tumours, which may negatively affect the clinical outcome and prognosis. Recent studies suggest that serum creatinine-cystatin C (Cr/CysC) ratio may be associated with sarcopenia, but this association lacks sufficient evidence in patients with gastrointestinal stromal tumours (GIST). Therefore, this study aimed to investigate whether the Cr/CysC ratio was associated with sarcopenia and recurrence-free survival (RFS) in patients with GIST. Materials and methods: The study retrospectively analysed 413 patients with GIST who underwent surgical resection from January 2016 to January 2020. The serum Cr/CysC ratio was determined as a proxy for sarcopenia by comparing it with various biomarkers and Cox multifactorial analysis was used to determine the relationship between Cr/CysC ratio and prognosis. Results: Serum Cr/CysC was positively correlated with skeletal muscle area (SMA) (r = 0.256, p < 0.001), skeletal muscle index (SMI) (r = 0.300, p < 0.001), and hand grip strength (HGS) (r = 0.251, p < 0.001). The area under the receiver operator characteristic curve for sarcopenic subjects with serum Cr/CysC ratio was significantly greater than other biomarkers (Cr/CysC: 0.840, CysC: 0.732, Cr: 0.518). The optimal cut-off value for Cr/CysC was 0.65, and patients in the high Cr/CysC group had a higher 3-year recurrence-free survival (RFS) than those in the low Cr/CysC group (92.72 vs. 72.46%, p < 0.001). Cox multifactorial analysis found that the Cr/CysC ratio was an independent risk factor for RFS in GIST patients (HR = 2.143, 95% CI: 1.431-5.459, p = 0.011). Conclusion: Serum Cr/CysC ratio has satisfactory and comparable diagnostic accuracy, and prognostic value for sarcopenia in patients with GIST. Therefore, it can be a simple and practical clinical tool to screen sarcopenia in GIST patients. However, further studies are required to validate these findings.

16.
Int J Biol Macromol ; 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36122778

RESUMO

Recently, the frequent salinity fluctuation has become a growing threat to fishes. However, the dynamic patterns of gene expression in response to salinity changes remain largely unexplored. In the present study, 18 RNA-Seq datasets were generated from gills of rainbow trout at different salinities, including 0 ‰, 6 ‰, 12 ‰, 18 ‰, 24 ‰ and 30 ‰. Based on the strict thresholds, we have identified 63, 1411, 2096, 1031 and 1041 differentially expressed genes in gills of rainbow trout through pairwise comparisons. Additionally, weighted gene co-expression network analysis was performed to construct 18 independent modules with distinct expression patterns. Of them, green and tan modules were found to be tightly related to salinity changes, several hub genes of which are known as the important regulators in taurine and glutamine metabolism. To further investigate their potential roles in response to salinity changes, taurine, glutamine, and their metabolism-related glutamic acid and α-ketoglutaric acid were accurately quantitated using liquid chromatography-tandem mass spectrometry analysis. Results clearly showed that their concentrations were closely associated with salinity changes. These findings suggested that taurine and glutamine play important roles in response to salinity changes in gills of rainbow trout, providing new insights into the molecular mechanism of fishes in salinity adaptation.

17.
Ultrasonics ; 127: 106846, 2022 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-36130436

RESUMO

The aircraft flight safety and efficiency are largely affected by the aircraft icing, whose characterization is of great significance to guiding the de-icing operation. This study targets a robust and quantitative characterization of icing with ultrasonic guided wave (UGW) of selective frequency-mode pair. Firstly, the frequency domain finite element (FDFE) model is developed to quantitatively analyze the reflection, transmission, and mode conversion of UGW with icing aluminum plate of different lengths and thickness, in order to pick the UGW with appropriate frequency-mode pair sensitive to icing. With the candidate UGW frequency-mode pair, the time domain finite element (TDFE) analysis is further performed to validate the effectiveness of FDFE and to optimize the pulse duration cycle for icing detection. Finally, based on the performed FDFE and TDFE analysis, experimental icing characterization is carried out with the UGW of the selected frequency-mode pair. Results show that the selected UGW at (1239 kHz, mode A1) with ice (0.5 mm thick) has achieved a high detection sensitivity based on the time-of-flight and good robustness against the random error, showing the great potential for the application of UGW to aircraft icing characterization.

18.
BMC Endocr Disord ; 22(1): 232, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36114534

RESUMO

BACKGROUND: Current evidence demonstrates that blood glucose fluctuation can be associated with depression and anxiety. The association among blood glucose fluctuation, traditional risk factors and emotional disorders in T2DM should be studied and clarified. METHODS: A total of 182 diabetic patients including 81 patients with depression or anxiety and 101 patients without emotional disorder were enrolled into this study. Data were obtained through medical history and questionnaire survey. Data were analyzed using appropriate statistical methods. RESULTS: The comparison results of basic information between the two groups showed that the differences of the proportion of female were statistically significant (p = 0.002). There was no statistical difference in laboratory examination indexes between the two groups, however, standard deviation of blood glucose (SDBG) and postprandial glucose excursion (PPGE) of the comorbidity group were significantly higher than that of control group (p = 0.032 and p = 0.037). The results of questionnaire survey showed that there were statistically significant differences in sleep quality, PSQI and dietary habit between the two groups (p < 0.001, p < 0.001 and p < 0.001). Stratified analysis results according to gender showed that the percentage of cognitive disorder, anxiety and depression in female group was significantly higher than that in male group (p = 0.001, p < 0.001 and p < 0.001). Mini-mental state examination (MMSE), self-rating anxiety scale (SAS) and patient health questionnaire (PHQ-9) score in female group were also higher than male group (p = 0.001, p < 0.001 and p < 0.001). Logistic regression analysis results showed that SDBG and sleep quality were associated with emotional disorders in T2DM (p = 0.040 and p < 0.001) and the OR values of these factors were 7.588 (1.097-52.069) and 4.428 (2.649-7.401). CONCLUSIONS: Blood glucose fluctuation and sleep quality are associated with the increased prevalence of depression and anxiety disorders in T2DM.


Assuntos
Diabetes Mellitus Tipo 2 , Transtornos do Sono-Vigília , Ansiedade/epidemiologia , Ansiedade/etiologia , Transtornos de Ansiedade/complicações , Transtornos de Ansiedade/etiologia , Glicemia , Depressão/epidemiologia , Depressão/etiologia , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/epidemiologia , Feminino , Humanos , Masculino , Prevalência , Qualidade do Sono , Transtornos do Sono-Vigília/epidemiologia , Transtornos do Sono-Vigília/etiologia
19.
Environ Health ; 21(1): 86, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36114578

RESUMO

BACKGROUND: Essential trace elements (ETEs), such as copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), selenium (Se), zinc (Zn), are very important elements for human health. METHODS: In this study, 89 drinking water samples and 85 highland barleys were collected from 48 villages in 11 townships, and the average daily dose (ADD) of ETEs were calculated, in addition, health effects of ETEs to rural residents in Luolong County, a typical Kashin-Beck disease (KBD) endemic area in Tibet, were assessed. RESULTS: The mean concentrations of Cu, Fe, Mn, Mo, Se, Zn in drinking water were 0.278 ± 0.264 µg·kg-1, 0.766 ± 0.312 µg·kg-1, 0.411 ± 0.526 µg·kg-1, 0.119 ± 0.223 µg·kg-1, 0.155 ± 0.180 µg·kg-1, and 0.804 ± 1.112 µg·kg-1, respectively; and mean concentrations of Cu, Fe, Mn, Mo, Se and Zn in highland barley were 3.550 ± 0.680 mg·kg-1, 81.17 ± 38.14 mg·kg-1, 14.03 ± 1.42 mg·kg-1, 0.350 ± 0.200 mg·kg-1, 0.0028 ± 0.0056 mg·kg-1, and 23.58 ± 3.10 mg·kg-1, respectively. The ADD of Cu in the study area was appropriate; the ADD of Fe and Mn in each township were higher than the maximum oral reference dose recommended by the National Health Commission of China, indicating that Fe and Mn had non-carcinogenic health risks; the ADD of Mo and Zn in 36.36% and 54.55% of the townships exceeded the maximum oral reference dose; and 72.73% of the townships had insufficient ADD of Se. The ADD of Mo, Cu and Se in different townships was significantly correlated with the prevalence of KBD. CONCLUSIONS: Therefore, in order to prevent and control the prevalence of KBD and ensure the health of local residents, it is necessary to reduce the intake of high concentrations of Fe, Mn and Zn in diet, as well as increase the intake of Mo, Cu, especially Se.


Assuntos
Água Potável , Doença de Kashin-Bek , Selênio , Oligoelementos , China/epidemiologia , Cobre , Dieta , Humanos , Ferro , Doença de Kashin-Bek/epidemiologia , Manganês , Molibdênio , Tibet/epidemiologia , Zinco/análise
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121873, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36126624

RESUMO

In this work, a rapid and sensitive strategy was developed to determine thiram (THI) and atrazine (ATZ) by surface-enhanced Raman scattering (SERS) technology. ß-cyclodextrin modified silver nanoparticles (ß-CD-AgNPs) were synthesized using ß-CD as a reducing agent and encapsulating agent under alkaline conditions and employed as SERS substrate. The existence of ß-CD can capture the molecules to form host-guest complex and fix molecular orientation in its cavity, thus ensuring the enhanced SERS signal intensity of THI and ATZ. The linear response extends from 2.56 × 10-8 to 2.56 × 10-3 mol/L for THI and 3.08 × 10-8 to 3.08 × 10-3 mol/L for ATZ, with the limits of detection (LOD) of 2.42 × 10-9 mol/L for THI and 7.26 × 10-9 mol/L for ATZ, respectively. The application of the proposed method in real samples including apple and water were investigated, and the results would help promote the application of SERS technology as a powerful analytical tool for detecting other pesticide residues. It is expected that this SERS strategy will provide great value for rapid detecting pesticide residues in food products and environmental systems.

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