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1.
J Colloid Interface Sci ; 629(Pt B): 750-762, 2022 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-36193619

RESUMO

Covalent organic frameworks (COFs), a class of flexibly tunable crystalline materials, have fascinating potential in photocatalytic hydrogen peroxide (H2O2) evolution under visible light irradiation. However, achieving efficient catalytic activity by tuning the composition of COFs and the linkages of building blocks is still a challenge. Herein, four imine-linked COFs with different numbers of hydroxy-functionalized are constructed to unveil the latent structure-activity relationship between the reversibility of bonding in supramolecular chemistry and the photocatalytic H2O2 performance. As the optimized material, TAPT-HTA-COF (1H-COF) containing single hydroxy group in aldehyde node exhibits a highest ordered structure and conjugation degree along and across the plane in the extended frameworks originating from the flexibly reversible iminol-to-ketoenamine tautomerism than others, which broadens the visible light absorption and accelerates the dissociation of photogenerated carriers in 1H-COF. These merits ensure that 1H-COF has the highest H2O2 yield (44.5 µmol L-1) and O2 two-electron reduction pathway among the four COFs under visible light irradiation (λ > 420 nm, 10 vol% isopropanol aqueous solution). At the same time, the long-range ordered framework of 1H-COF is well preserved during the photocatalytic H2O2 evolution process assisted by the proton-induced tautomerization. This work facilitates the design and development of COF-based photocatalysts in the evolution of H2O2.

2.
Nano Lett ; 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-36194752

RESUMO

The application of drug-loaded nanodroplets is still limited by their insufficient accumulation owing to the enhanced permeability and retention (EPR) effect failure in cancer therapy. To overcome these limitations, we propose an alternative magnetic particle-encapsulated nanodroplet (MPE-ND) with outstanding biosafety and magnetic targeting by encapsulating fluorinated Fe3O4-SiO2 nanoparticles inside the liquid core of the nanodroplets. Meanwhile, doxorubicin (DOX) can be stably loaded into the shell through both electrostatic and hydrophobic interactions to obtain drug-loaded MPE-NDs. Both in vitro and in vivo experiments have consistently demonstrated that drug-loaded MPE-NDs can significantly increase the local drug concentration and enhance the damage of tumor tissues through acoustic droplet vaporization under a static magnetic field (eADV therapy). Histological examination reveals that eADV therapy efficiently suppresses tumor proliferation by inducing apoptosis, destroying supply vessels, and inhibiting neovascularization. Drug-loaded MPE-NDs can be expected to open a new gateway for ultrasound-triggered drug delivery and cancer treatment.

3.
Reprod Biol Endocrinol ; 20(1): 137, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36088329

RESUMO

BACKGROUND: We aimed to compare the clinical and perinatal outcomes of patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment with either an early-follicular long-acting gonadotropin-releasing hormone agonist (GnRH-a) long protocol (EFLL) or a midluteal short-acting GnRH-a long protocol (MLSL). METHODS: This single-center, retrospective study, included patients with PCOS who underwent IVF/ICSI from January 2013 to June 2019 at the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Patients underwent either MLSL (1179 cycles) or EFLL (2390 cycles). The primary outcomes were pregnancy outcomes, perinatal and maternal complications. RESULTS: Fresh embryo transfer (59.12% vs. 55.47%, P = 0.038), clinical pregnancy (75.23% vs. 53.82%, P = 0.001), and live birth rates (63.27% vs. 42.05%, P = 0.010) were higher in the EFLL group. However, the proportion of patients "freezing all" for high risk of ovarian hyperstimulation syndrome (OHSS) (24.27% vs. 32.06%, P = 0.001) and ectopic pregnancy (1.51% vs. 5.97%, P = 0.002) were lower in the EFLL group than in the MLSL group. The incidence of gestational diabetes was higher in the EFLL group than in the MLSL group (5.08% vs. 1.42%, RR 3.714, 95% confidence interval (CI) 1.474-9.360, P = 0.003). There were no significant differences in the incidence of hypertension, premature rupture of membranes, placenta previa, congenital heart disease, or neonatal weight between the two groups. Logistic regression results showed that age (OR 0.966, 95% CI 0.941-0.993, P = 0.013), treatments (OR 2.380, 95% CI 1.833-3.089, P = 0.001), and endometrial thickness on trigger day (OR 1.115, 95% CI 1.070-1.162, P = 0.001) were correlated with clinical pregnancy. Pre-pregnancy BMI (OR 1.098, 95% CI 1.002-1.204, P = 0.046), fasting plasma glucose (FPG) (OR 3.096, 95% CI 1.900-5.046, P = 0.001), and treatments (OR 3.458, 95% CI 1.359-8.800, P = 0.009) were correlated with gestational diabetes mellitus (GDM). Treatments (OR 0.291, 95% CI 0.148-0.575, P = 0.001) and endometrial thickness on trigger day (OR 0.834, 95% CI 0.722-0.962, P = 0.013) were correlated with ectopic pregnancy. CONCLUSION: The early-follicular long-acting GnRH agonist long protocol can be used as an ideal assisted reproductive technology (ART) pregnancy assistance program for patients with PCOS, but obese patients should be encouraged to lose weight before ART treatments to reduce the risk of GDM.


Assuntos
Síndrome do Ovário Policístico , Gravidez Ectópica , Feminino , Fertilização In Vitro/métodos , Hormônio Liberador de Gonadotropina/uso terapêutico , Humanos , Recém-Nascido , Masculino , Indução da Ovulação/métodos , Síndrome do Ovário Policístico/tratamento farmacológico , Gravidez , Gravidez Ectópica/etiologia , Estudos Retrospectivos , Sêmen
4.
Cell Metab ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36103895

RESUMO

The tumor microenvironment (TME) is a unique niche governed by constant crosstalk within and across all intratumoral cellular compartments. In particular, intratumoral high potassium (K+) has shown immune-suppressive potency on T cells. However, as a pan-cancer characteristic associated with local necrosis, the impact of this ionic disturbance on innate immunity is unknown. Here, we reveal that intratumoral high K+ suppresses the anti-tumor capacity of tumor-associated macrophages (TAMs). We identify the inwardly rectifying K+ channel Kir2.1 as a central modulator of TAM functional polarization in high K+ TME, and its conditional depletion repolarizes TAMs toward an anti-tumor state, sequentially boosting local anti-tumor immunity. Kir2.1 deficiency disturbs the electrochemically dependent glutamine uptake, engendering TAM metabolic reprogramming from oxidative phosphorylation toward glycolysis. Kir2.1 blockade attenuates both murine tumor- and patient-derived xenograft growth. Collectively, our findings reveal Kir2.1 as a determinant and potential therapeutic target for regaining the anti-tumor capacity of TAMs within ionic-imbalanced TME.

5.
Microsc Microanal ; : 1-8, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36101003

RESUMO

Electron tomography (ET) has gained increasing attention for the 3D characterization of nanoparticles. However, the missing wedge problem due to a limited tilt angle range is still the main challenge for accurate reconstruction in most experimental TEM setups. Advanced algorithms could in-paint or compensate to some extent the missing wedge artifacts, but cannot recover the missing structural information completely. 360° ET provides an option to solve this problem by tilting a needle-shaped specimen over the full tilt range and thus filling the missing information. However, sample preparation especially for fine powders to perform full-range ET is still challenging, thus limiting its application. In this work, we propose a new universal sample preparation method that enables the transfer of selected individual nanoparticle or a few separated nanoparticles by cutting a piece of carbon film supporting the specimen particles and mounting them onto the full-range tomography holder tip with the help of an easily prepared sharp tungsten tip. This method is demonstrated by 360° ET of Pt@TiO2 hollow cage catalyst showing high quality reconstruction without missing wedge.

6.
Nutrients ; 14(17)2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36079733

RESUMO

Obesity is frequently associated with dysregulated lipid metabolism and lipotoxicity. Inonotus hispidus (Bull.: Fr.) P. Karst (IH) is an edible and medicinal parasitic mushroom. In this study, after a systematic analysis of its nutritional ingredients, the regulatory effects of IH on lipid metabolism were investigated in mice fed a high-fat diet (HFD). In HFD-fed mice, IH reversed the pathological state of the liver and the three types of fat and significantly decreased the levels of low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglycerides (TG), and leptin (LEP) and increased the level of high-density liptein cholesterol (HDL-C) in serum. Meanwhile, IH ameliorated liver damage by reducing alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin (IL)-1ß, IL-6, tumor necrosis factor-alpha (TNF-α), and plasminogen activator inhibitor-1 (PAI-1) levels in the liver and serum. Compared with HFD-fed mice, IH significantly modulated the gut microbiota, changed the relative abundances of microflora at different taxonomic levels, and regulated lipid levels. The results showed that 30 differential lipids were found. Results from Western blotting confirmed that IH regulated the nuclear factor erythroid-2 related factor 2 (Nrf2)/nuclear factor-kappa B (NF-κB) signaling pathway and oxidative stress. This study aimed to provide experimental evidence for the applicability of IH in obesity treatment.


Assuntos
Dieta Hiperlipídica , Hiperlipidemias , Animais , Colesterol/metabolismo , Dieta Hiperlipídica/efeitos adversos , Hiperlipidemias/metabolismo , Inflamação/metabolismo , Inonotus , Fígado/metabolismo , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , NF-kappa B/metabolismo , Obesidade/metabolismo , Estresse Oxidativo , Transdução de Sinais
7.
Molecules ; 27(17)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36080200

RESUMO

In this paper, two new iridium (III) complexes, [Ir(ppy)2(ipbp)](PF6) (Ir1) (ppy = 2-phenylpyridine, ipbp = 3-(1H-imidazo[4,5-f][1,10]phenanthrolin-2yl)-4H-chromen-4-one) and [Ir(bzq)2(ipbp)](PF6) (Ir2) (bzq = benzo[h]quinolone), were synthesized and characterized. The cytotoxicity of the complexes against human colon cancer HCT116 and normal LO2 cells was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The complexes Ir1 and Ir2 show high cytotoxic efficacy toward HCT116 cells with a low IC50 value of 1.75 ± 0.10 and 6.12 ± 0.2 µM. Interestingly, Ir1 only kills cancer cells, not normal LO2 cells (IC50 > 200 µM). The inhibition of cell proliferation and migration were investigated by multiple tumor spheroid (3D) and wound healing experiments. The cellular uptake was explored under a fluorescence microscope. The intracellular reactive oxygen species (ROS), change of mitochondrial membrane potential, glutathione (GSH) and adenine nucleoside triphosphate (ATP) were studied. Apoptosis and cell cycle arrest were performed by flow cytometry. The results show that the complexes induce early apoptosis and inhibit the cell proliferation at the G0/G1 phase. Additionally, the apoptotic mechanism was researched by Western blot analysis. The results obtained demonstrate that the complexes cause apoptosis in HCT116 cells through ROS-mediated mitochondrial dysfunction and the inhibition of PI3K/AKT signaling pathways.


Assuntos
Antineoplásicos , Neoplasias do Colo , Complexos de Coordenação , Antineoplásicos/farmacologia , Apoptose , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias do Colo/tratamento farmacológico , Células HCT116 , Humanos , Irídio/farmacologia , Fosfatidilinositol 3-Quinases , Espécies Reativas de Oxigênio/metabolismo
8.
Signal Transduct Target Ther ; 7(1): 311, 2022 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-36068203

RESUMO

Indoleamine 2,3-dioxygenase 1 (IDO1), the enzyme that catabolizes tryptophan (Trp) metabolism to promote regulatory T cells (Tregs) and suppress CD8+ T cells, is regulated by several intrinsic signaling pathways. Here, we found that tobacco smoke, a major public health concern that kills 8 million people each year worldwide, induced IDO1 in normal and malignant lung epithelial cells in vitro and in vivo. The carcinogen nicotine-derived nitrosaminoketone (NNK) was the tobacco compound that upregulated IDO1 via activation of the transcription factor c-Jun, which has a binding site for the IDO1 promoter. The NNK receptor α7 nicotinic acetylcholine receptor (α7nAChR) was required for NNK-induced c-Jun activation and IDO1 upregulation. In A/J mice, NNK reduced CD8+ T cells and increased Tregs. Clinically, smoker patients with non-small-cell lung cancer (NSCLC) exhibited high IDO1 levels and low Trp/kynurenine (Kyn) ratios. In NSCLC patients, smokers with lower IDO1 responded better to anti-PD1 antibody treatment than those with higher IDO1. These data indicate that tobacco smoke induces IDO1 to catabolize Trp metabolism and immune suppression to promote carcinogenesis, and lower IDO1 might be a potential biomarker for anti-PD1 antibodies in smoker patients, whereas IDO1-high smoker patients might benefit from IDO1 inhibitors in combination with anti-PD1 antibodies.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Poluição por Fumaça de Tabaco , Animais , Linfócitos T CD8-Positivos/metabolismo , Carcinógenos/toxicidade , Humanos , Indolamina-Pirrol 2,3,-Dioxigenase/genética , Neoplasias Pulmonares/induzido quimicamente , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Camundongos , Tabaco/metabolismo , Triptofano
9.
Front Cell Infect Microbiol ; 12: 995705, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072220

RESUMO

Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming isolation methods, the relatively poor detection sensitivity and high measurement bias for traceable matrix-rich samples need to be improved. Here, we thus developed a droplet digital PCR (ddPCR) assay for accurate, sensitive detection and quantification of V. dahliae and V. longisporum. We compared the analytical and diagnostic performance in detail of ddPCR and the corresponding qPCR assay against the genomic DNA (gDNA) of the two fungi from cultures and field samples. In our study, the species specificity, quantification linearity, analytical sensitivity, and measurement viability of the two methods were analyzed. The results indicated that ddPCR using field samples enhanced diagnostic sensitivity, decreased quantification bias, and indicated less susceptibility to inhibitors compared with qPCR. Although ddPCR was as sensitive as qPCR when using gDNA from cultures of V. dahliae and V. longisporum, its detection rates using field samples were much higher than those of qPCR, potentially due to the inhibition from residual matrix in the extracts. The results showed that digital PCR is more sensitive and accurate than qPCR for quantifying trace amounts of V. dahliae and V. longisporum and can facilitate management practices to limit or prevent their prevalence.


Assuntos
Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase em Tempo Real/métodos , Verticillium
10.
Cell Death Dis ; 13(9): 767, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36064939

RESUMO

Glioblastoma multiforme (GBM) is the most common tumor of the human central nervous system. Aerobic glycolysis has been strongly related to tumor development and malignant behavior. In this study, we found that MBNL1, circNTRK2, and NTRK2-243aa were markedly downregulated and inhibited glycolysis in GBM, whereas PAX5 was upregulated and promoted glycolysis. Functionally, MBNL1 promoted the expression of circNTRK2 by binding to NTRK2 pre-mRNA, as validated using RNA pull-down and nascent RNA immunoprecipitation assays. Mass spectrometry, western blotting, and immunofluorescence staining methods were used to detect the expression of NTRK2-243aa. NTRK2-243aa-encoded by circNTRK2-phosphorylated PAX5 at Y102, leading to the attenuation of the half-life of PAX5, as validated by in vitro kinase and MG132 rescue assays. Besides, PAX5 transcriptionally facilitated the expression of PKM2 and HK2 by binding to their promoter regions, as verified by luciferase reporter and chromatin immunoprecipitation assays. Finally, overexpression of MBNL1 and circNTRK2 combined with PAX5 knockdown effectively inhibited the formation of GBM xenograft tumors and significantly prolonged the survival of orthotopic nude mice. We have delineated that the MBNL1/circNTRK2/PAX5 pathway plays a crucial role in regulating GBM glycolysis and could provide potential targets and alternative strategies for the treatment of GBM.


Assuntos
Neoplasias do Sistema Nervoso Central , Proteínas de Ligação a DNA , Glioblastoma , Glicoproteínas de Membrana , Fator de Transcrição PAX5 , Proteínas de Ligação a RNA , Receptor trkB , Animais , Linhagem Celular Tumoral , Proliferação de Células , Neoplasias do Sistema Nervoso Central/genética , Neoplasias do Sistema Nervoso Central/metabolismo , Neoplasias do Sistema Nervoso Central/patologia , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Regulação Neoplásica da Expressão Gênica , Glioblastoma/genética , Glioblastoma/metabolismo , Glioblastoma/patologia , Glicólise/genética , Glicólise/fisiologia , Humanos , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Nus , Fator de Transcrição PAX5/genética , Fator de Transcrição PAX5/metabolismo , RNA/genética , RNA/metabolismo , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Receptor trkB/genética , Receptor trkB/metabolismo , Transdução de Sinais/genética , Transdução de Sinais/fisiologia
11.
Front Microbiol ; 13: 895779, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36051755

RESUMO

Transcription and replication of the influenza A virus (IAV) genome take place in the nucleus of infected cells, which rely on host factors to aid viral ribonucleoprotein (vRNP) to cross the nuclear pore complex (NPC) and complete the bidirectional nucleocytoplasmic trafficking. Here, we showed that nucleoporin 85 (NUP85), a component of NPC, interacted with RNP subunits polymerase basic 1 (PB1) and polymerase basic 2 (PB2) in an RNA-dependent manner during IAV infection. Knockdown of NUP85 delayed the nuclear import of vRNP, PB1 and PB2, inhibiting polymerase activity and ultimately suppressing viral replication. Further analysis revealed that NUP85 assisted the binding of PB1 to nuclear transport factor Ran-binding protein 5 (RanBP5) and the binding of PB2 to nuclear transport factor importin α1 and importin α7. We also found that NUP85 expression was downregulated upon IAV infection. Together, our study demonstrated that NUP85 positively regulated IAV infection by interacting with viral PB1 and PB2, which may provide new insight into the process of vRNP nuclear import and a novel target for effective antivirals.

12.
Chin Med ; 17(1): 103, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36056398

RESUMO

BACKGROUND: The limited therapeutic outcomes of atherosclerosis (AS) have allowed, traditional Chinese medicine has been well established as an alternative approach in ameliorating AS and associated clinical syndromes. Clinically, Tongsaimai tablet (TSMT), a commercial Chinese patent medicine approved by CFDA, shows an obvious therapeutic effect on AS treatment. However, its effective mechanism and quality control still need thorough and urgent exploration. METHODS: The mice were orally administered with TSMT and their serum was investigated for the absorbed compounds using serum pharmacochemistry via the UPLC-Q-Exactive Orbitrap/MS analysis was employed to investigate these absorbed compounds in serum of mice orally administrated with TSMT. Based on these absorbed prototype compounds in serum derived from TSMT, a component-target-disease network was constructed using network pharmacology strategy, which elucidated the potential bioactive components, effective targets, and molecular mechanisms of TSMT against AS. Further, the screened compounds from the component-target network were utilized as the quality control (QC) markers, determining multi-component content determination and HPLC fingerprint to assess quality of nine batches of TSMT samples. RESULTS: A total of 164 individual components were identified in TSMT. Among them, 29 prototype compounds were found in serum of mice administrated with TSMT. Based on these candidate prototype components, 34 protein targets and 151 pathways related to AS were predicted, and they might significantly exhibit potential anti-AS mechanisms via synergistic regulations of lipid regulation, shear stress, and anti-inflammation, etc. Five potentially bioactive ingredients in TSMT, including Ferulic acid, Liquiritin, Senkyunolide I, Luteolin and Glycyrrhizic acid in quantity not less than 1.2798, 0.4716, 0.5419, 0.1349, 4.0386 mg/g, respectively, screened from the component-target-pathway network. Thereby, these indicated that these five compounds of TMST which played vital roles in the attenuation of AS could serve as crucial marker compounds for quality control. CONCLUSIONS: Overall, based on the combination of serum pharmacochemistry and network pharmacology, the present study firstly provided a useful strategy to establish a quality assessment approach for TSMT by screening out the potential anti-AS mechanisms and chemical quality markers.

13.
Biosaf Health ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36091480

RESUMO

The pandemic caused by SARS-CoV-2 has led to unprecedented social and economic disruption. Many Nucleic acid testing (NAT) laboratories in China have been established to control the epidemic better. This proficiency testing (PT) aims to evaluate the participants' performance in qualitative and quantitative SARS-CoV-2 NAT and to explore the factors that contribute to differences in detection capabilities. Two different concentrations of RNA samples (A, B) were used for quantitative PT. Pseudovirus samples D, E (different concentration) and negative sample (F) were used for qualitative PT. 50 data sets were reported for qualitative PT, of which 74.00% were entirely correct for all samples. Forty-two laboratories participated in the quantitative PT. 37 submitted all gene results, of which only 56.76% were satisfactory. For qualitative detection, it is suggested that laboratories should strengthen personnel training, select qualified detection kits, and reduce cross-contamination to improve detection accuracy. For quantitative detection, the results of the reverse transcription digital PCR (RT-dPCR) method were more comparable and reliable than those of reverse transcription quantitative PCR (RT-qPCR). The copy number concentration of ORF1ab and N in samples A and B scattered in 85, 223, 50, and 106 folds, respectively. The differences in the quantitative result of RT-qPCR was mainly caused by the non-standard use of reference materials and the lack of personnel operating skills. Comparing the satisfaction of participants participating in both quantitative and qualitative proficiency testing, 95.65% of the laboratories with satisfactory quantitative results also judged the qualitative results correctly, while 85.71% of the laboratories with unsatisfactory quantitative results were also unsatisfied with their qualitative judgments. Therefore, the quantitative ability is the basis of qualitative judgment. Overall, participants from hospitals reported more satisfactory results than those from enterprises and universities. Therefore, surveillance, daily qualitiy control and standardized operating procedures should be strengthened to improve the capability of SARS-CoV-2 NAT.

14.
J Invertebr Pathol ; 194: 107825, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36096179

RESUMO

The entomopathogenic fungus Cordyceps fumosorosea IF-1106 is a potential biocontrol agent with high pathogenicity to the aphid Myzus persicae. We extracted the crude toxin protein from a liquid culture broth of an isolated C. fumosorosea strain using the ammonium sulfate precipitation method, and its toxicity to Myzus persicae was measured by injection, oral exposure, and topical exposure. The crude toxin protein of C. fumosorosea had insecticidal activity against M. persicae. Body cavity injection and oral exposure had significantly higher insecticidal activity against adults than contact sprays. The highest cumulative corrected mortality of adults after injection was 81.85 ± 13.45 %, and the highest cumulative corrected mortality of adults after ingestion was 85.45 ± 11.88 %. The proportion of plasmatocytes in adult blood lymphocytes reached the highest at 3 days after injection and feeding, and the proportion of granulocytes was the highest at 2 days after injection and feeding. These data confirmed the toxicity of the crude toxin protein of C. fumosorosea toxin to M. persicae and helped clarify the pathogenic mechanism of the strain. Population management of M. persicae may be possible by using a natural toxic compound produced by C. fumosorosea that is selective to this pest species.

15.
Polymers (Basel) ; 14(18)2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36145862

RESUMO

To develop a novel asymmetric nanofiber membrane with antioxidant and antibacterial activities, biocompatible polyvinyl pyrrolidone (PVP) and polycaprolactone (PCL) were used as carriers to load water-soluble tea tree extract (TTE) and hydrophobic tea tree oil (TTO) via electrospinning and electrostatic spraying, respectively, which was named as TTE-PVP-3/TTO-PCL. The results show that uniform TTE-PVP nanofibers with an average diameter of 95 ± 27 nm could be obtained when the mass ratio of TTE to PVP was set as 1:3. Homogeneous TTO/PCL microspheres with an average size of 4.38 ± 0.79 µm could be obtained when the propulsion speed was 0.08 mm/min and the voltage was 10 KV. The activity study showed that TTE could only improve the antioxidant activity of TTE-PVP-3/TTO-PCL, while TTO could improve the antibacterial activity effectively. Under experimental conditions, the inhibition zones of TTE-PVP-3/TTO-PCL against Staphylococcus aureus and Escherichia coli were 7.50 ± 0.48 mm and 9.55 ± 0.45 mm, respectively, and its scavenging rates for 2,2-diphenylpicrylhydrazyl (DHPP) and hydroxyl radical were 59.79 ± 4.10% and 61.02 ± 4.95%, respectively. In conclusions, TTE-PVP-3/TTO PCL can be potentially used as a new kind of anti-oxidative and antibacterial wound dressings.

16.
Polymers (Basel) ; 14(18)2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36145866

RESUMO

Hydrogels based on nanocomposites (NC) structure have acquired a great deal of interest, but they are still limited by relatively low mechanical strength, inevitably losing elasticity when applied below subzero temperatures, due to the formation of ice crystallization. In this study, an anti-freezing and mechanically strong Laponite NC organohydrogel was prepared by a direct solvent replacement strategy of immersing Laponite NC pre-hydrogel into ethylene glycol (EG)/water mixture solution. In the organohydrogel, a part of water molecules was replaced by EG, which inhibited the formation of ice crystallization even at extremely low temperatures. In addition, the formation of hydrogen bonds between Laponite and the monomers of N-isopropylacrylamide (NIPAM) and hydroxyethyl acrylate (HEA) endowed the organohydrogels with high mechanical strength and toughness. The NC organohydrogel can maintain its mechanical flexibility even at -25 °C. The compressive stress, tensile stress, and elongation at the break of N5H5L reached 3871.71 kPa, 137.05 kPa, and 173.39%, respectively, which may be potentially applied as ocean probes in low temperature environment.

17.
Materials (Basel) ; 15(18)2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36143495

RESUMO

The mechanical properties of ultra-high performance concrete (UHPC) made of manufactured sand (MS) under four curing methods (steam, standard, sprinkler and saturated Ca(OH)2) were investigated via compressive, flexural and uniaxial tensile tests, and the bond strength of steel fiber and manufactured sand UHPC (MSUHPC) matrix. Based on the analysis of the microstructure, the influence mechanism of curing methods on the mechanical properties of materials was explored. The results showed that the early compressive strength of MSUHPC under steam curing (SM) is much higher than that of the other three curing methods, but the difference gradually decreases with the increase of age. The compressive strength of MSUHPC under SM is higher than that of river sand UHPC (RSUHPC). The bending strength and compressive strength of MSUHPC under different curing methods are similar, and the bending strength of 28 days steam cured samples is the highest. The uniaxial tensile properties of MSUHPC did not show significant difference under standard curing (SD), sprinkler curing (SP) and saturated Ca(OH)2 curing (CH), and the uniaxial tensile properties of MSUHPC under SM are slightly better than RSUHPC. The ultimate bond strength and fiber pullout energy of steel fiber and MSUHPC increase with the development of age. The bond strength and fiber pullout work of SM is higher than those of the other three curing methods, but there are lower increases in the later stage than that of the other three curing methods.

18.
N Engl J Med ; 387(13): 1173-1184, 2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36066078

RESUMO

BACKGROUND: Many persons with a history of smoking tobacco have clinically significant respiratory symptoms despite an absence of airflow obstruction as assessed by spirometry. They are often treated with medications for chronic obstructive pulmonary disease (COPD), but supporting evidence for this treatment is lacking. METHODS: We randomly assigned persons who had a tobacco-smoking history of at least 10 pack-years, respiratory symptoms as defined by a COPD Assessment Test score of at least 10 (scores range from 0 to 40, with higher scores indicating worse symptoms), and preserved lung function on spirometry (ratio of forced expiratory volume in 1 second [FEV1] to forced vital capacity [FVC] ≥0.70 and FVC ≥70% of the predicted value after bronchodilator use) to receive either indacaterol (27.5 µg) plus glycopyrrolate (15.6 µg) or placebo twice daily for 12 weeks. The primary outcome was at least a 4-point decrease (i.e., improvement) in the St. George's Respiratory Questionnaire (SGRQ) score (scores range from 0 to 100, with higher scores indicating worse health status) after 12 weeks without treatment failure (defined as an increase in lower respiratory symptoms treated with a long-acting inhaled bronchodilator, glucocorticoid, or antibiotic agent). RESULTS: A total of 535 participants underwent randomization. In the modified intention-to-treat population (471 participants), 128 of 227 participants (56.4%) in the treatment group and 144 of 244 (59.0%) in the placebo group had at least a 4-point decrease in the SGRQ score (difference, -2.6 percentage points; 95% confidence interval [CI], -11.6 to 6.3; adjusted odds ratio, 0.91; 95% CI, 0.60 to 1.37; P = 0.65). The mean change in the percent of predicted FEV1 was 2.48 percentage points (95% CI, 1.49 to 3.47) in the treatment group and -0.09 percentage points (95% CI, -1.06 to 0.89) in the placebo group, and the mean change in the inspiratory capacity was 0.12 liters (95% CI, 0.07 to 0.18) in the treatment group and 0.02 liters (95% CI, -0.03 to 0.08) in the placebo group. Four serious adverse events occurred in the treatment group, and 11 occurred in the placebo group; none were deemed potentially related to the treatment or placebo. CONCLUSIONS: Inhaled dual bronchodilator therapy did not decrease respiratory symptoms in symptomatic, tobacco-exposed persons with preserved lung function as assessed by spirometry. (Funded by the National Heart, Lung, and Blood Institute and others; RETHINC ClinicalTrials.gov number, NCT02867761.).


Assuntos
Broncodilatadores , Doença Pulmonar Obstrutiva Crônica , Agonistas de Receptores Adrenérgicos beta 2 , Antibacterianos/uso terapêutico , Broncodilatadores/uso terapêutico , Volume Expiratório Forçado , Glucocorticoides/uso terapêutico , Glicopirrolato , Humanos , Pulmão , Doença Pulmonar Obstrutiva Crônica/tratamento farmacológico , Tabaco , Resultado do Tratamento
19.
Sci Adv ; 8(37): eabq4578, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36103530

RESUMO

The interface between magnetic material and superconductors has long been predicted to host unconventional superconductivity, such as spin-triplet pairing and topological nontrivial pairing state, particularly when spin-orbital coupling (SOC) is incorporated. To identify these unconventional pairing states, fabricating homogenous heterostructures that contain such various properties are preferred but often challenging. Here, we synthesized a trilayer-type van der Waals heterostructure of MnTe/Bi2Te3/Fe(Te, Se), which combined s-wave superconductivity, thickness-dependent magnetism, and strong SOC. Via low-temperature scanning tunneling microscopy, we observed robust zero-energy states with notably nontrivial properties and an enhanced superconducting gap size on single unit cell (UC) MnTe surface. In contrast, no zero-energy state was observed on 2-UC MnTe. First-principle calculations further suggest that the 1-UC MnTe has large interfacial Dzyaloshinskii-Moriya interaction and a frustrated AFM state, which could promote noncolinear spin textures. It thus provides a promising platform for exploring topological nontrivial superconductivity.

20.
Cell Biol Int ; 2022 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-36177490

RESUMO

The signaling pathways are highly conserved between Drosophila and mammals concerning intestinal development, regeneration, and disease. The powerful genetic tools of Drosophila make it a valuable and convenient alternative to answer basic biological questions that can not be addressed using mammalian models. In this review, we discuss recent advances in how we use fly midgut to answer the following key questions: (1) How intestine stem cell niches are established; (2) which factors control asymmetric division of stem cells; (3) how intestinal cells interact with environmental factors, such as tissue damage, microbiota, and diet; (4) how to screen aging/cancer-related factors or drugs by fly intestine stem cells.

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