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1.
Artigo em Inglês | MEDLINE | ID: mdl-31487886

RESUMO

The filling ratio (FR) of a carrier has an influence on the pollutant removal of the aerobic moving bed biofilm reactor (MBBR). However, the effect of the polyethylene (PE) carrier FR on the performance and microbial characteristics of the denitrifying MBBR for the treatment of wastewater treatment plant (WWTP) effluent has not been extensively studied. A bench-scale denitrifying MBBR was set up and operated with PE carrier FRs of 20%, 30%, 40%, and 50% for the degradation of chemical oxygen demand (COD) and nitrogen from WWTP effluent at 12 h hydraulic retention time (HRT). The nitrate removal rates with FRs of 20%, 30%, 40%, and 50% were 94.3 ± 3.9%, 87.7 ± 7.3%, 89.7 ± 11.6%, and 94.6 ± 4.0%, and the corresponding denitrification rates (rNO3--N) were 8.0 ± 5.6, 11.3 ± 4.6, 11.6 ± 4.6, and 10.0 ± 4.9 mg NO3--N/L/d, respectively. Nitrous oxide reductase (nosZ) gene-based terminal restriction fragment length polymorphism (T-RFLP) analysis illustrated that the highest functional diversity (Shannon's diversity index, H') of biofilm microbial community was obtained at 30% FR. The quantitative polymerase chain reaction (qPCR) results indicated that the abundance of nitrate reductase (narG) and nosZ genes at 30% FR was significantly higher than that at 20% FR, and no significant changes were observed at 40% and 50% FRs. Thus, 30% FR was recommended as the optimal carrier FR for the denitrifying MBBR.

2.
Biochim Biophys Acta Mol Basis Dis ; : 165554, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31513833

RESUMO

Activation of interferon (IFN)-I signaling in B cells contributes to the pathogenesis of systemic lupus erythematosus (SLE). Recent studies have shown that myeloid-derived suppressor cells (MDSCs) significantly expand in SLE patients and lupus-prone MRL/lpr mice and contribute to the pathogenesis of SLE. However, the role of SLE-derived MDSCs in regulating IFN-I signaling activation of B cells remains unknown. Here, we demonstrate that expansions of MDSCs, including granulocyte (G)-MDSCs and monocytic (M)-MDSCs, during the progression of SLE were correlated with the IFN-I signature of B cells. Interestingly, G-MDSCs from MRL/lpr mice, but not M-MDSCs, could significantly promote IFN-I signaling activation of B cells and contribute to the pathogenesis of SLE. Mechanistically, we identified that the long non-coding RNA NEAT1 was over-expressed in G-MDSCs from MRL/lpr mice and could induce the promotion of G-MDSCs on IFN-I signaling activation of B cells through B cell-activating factor (BAFF) secretion. Importantly, NEAT1 deficiency significantly attenuated the lupus symptoms in pristane-induced lupus mice. In addition, there was a positive correlation between NEAT1 and BAFF with the IFN signature in SLE patients. In conclusion, G-MDSCs may contribute to the IFN signature in SLE B cells through the NEAT1-BAFF axis, highlighting G-MDSCs as a potential therapeutic target to treat SLE.

3.
Nanoscale ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502627

RESUMO

Targeting is vital for precise positioning and efficient therapy, and integrated platforms for diagnosis and therapy have attracted more and more attention. Herein, we established dual-template molecularly imprinted polymer (MIP) coated fluorescent silicon nanoparticles (Si NPs) by using the linear peptide of the extracellular region of human epidermal growth factor receptor-2 (HER2) and adopting doxorubicin (DOX) as templates for targeted imaging and targeted therapy. Benefiting from the epitope imprinting approach, the imprinted sites generated by peptides on the MIP surface can be employed for recognizing the corresponding protein, which allowed the MIP to specifically and actively target HER2-positive breast cancer cells. Because of its ability to identify breast cancer cells, the MIP was applied for targeted fluorescence imaging by taking advantage of the excellent fluorescence properties of Si NPs, and the DOX-loaded MIP (MIP@DOX) can act as a therapeutic probe to effectively target and kill breast cancer cells. In fluorescence images, the targeting of the MIP promoted more uptake of the nanoparticles by cells than the non-imprinted polymer (NIP), so HER2-positive breast cancer cells incubated with the MIP exhibited stronger fluorescence, and there was no significant difference in fluorescence when HER2-negative cells and normal cells were respectively hatched with the MIP and NIP. Importantly, the cell viability was evaluated to demonstrate targeted accumulation and therapy of MIP@DOX for breast cancer cells. The nanoplatform for diagnosis and therapy combined the high sensitivity of fluorescence with the high selectivity of the molecular imprinting technique, which holds vital potential in targeted imaging and targeted therapy in vitro.

4.
Artif Cells Nanomed Biotechnol ; 47(1): 3671-3676, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31496296

RESUMO

Accumulating studies showed that microRNAs are maintaining a variety of important biological processes but the underlying mechanism in proliferation and tumourigenicity is unclear. In this study we show that miR-342 expression in bone marrow and patients' sera of childhood acute myeloid leukemia (AML) was both significantly higher than those in the corresponding normal controls. Functional assays demonstrated that forced expression of miR-342 significantly suppresses AML cell proliferation and G1/S transition of leukemia cells. Mechanistically, bioinformatics prediction and luciferase reporter assay identified N-a-acetyltransferase 10 protein (Naa10p) as a direct molecular target of miR-342, Naa10p siRNA significantly repressed cell proliferation and increased cell apoptosis. In conclusion, our study confirmed that miR-342/Naa10p plays key roles in AML progression, providing insights into underlying mechanisms of AML pathogenesis and also a potential therapeutic target for this malignancy.

5.
Reprod Biol Endocrinol ; 17(1): 69, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31443713

RESUMO

PURPOSE: Endometriosis is one of the most common, difficult, and complicated gynecological disorders. Vascular cell adhesion molecule 1 (VCAM-1) has been reported to be aberrantly expressed in patients with endometriosis. However, the exact role and mechanism of VCAM-1 in endometriosis remains unclear. METHODS: The expression of transforming growth factor beta 1 (TGF-ß1) and VCAM-1 was determined by quantitative real-time polymerase chain reaction and western blotting. Human endometriotic cells were cultured and their responsiveness to TGF-ß1 was evaluated by Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine, and transwell migration and invasion assays. RESULTS: The levels of TGF-ß1 and VCAM-1 mRNA were upregulated in the endometriotic tissues. Knockdown of TGF-ß1 in endometriotic cyst stromal cells caused a marked inhibition of cell proliferation, migration, and invasion. Treatment of endometriotic cyst stromal cells with TGF-ß1 resulted in an obvious promotion of cell proliferation, migration, and invasion, and strikingly increased the protein expression of VCAM-1. Silencing of Smad3 abated TGF-ß1-stimulated VCAM-1 expression. Furthermore, the promoting effects of TGF-ß1 on the proliferation, migration, and invasion of endometriotic cyst stromal cells were blocked by silencing of VCAM-1. CONCLUSION: Knockdown of VCAM-1 impedes TGF-ß1-mediated proliferation, migration, and invasion of endometrial cells, thereby indicating that VCAM-1 may serve as a therapeutic target for endometriosis.

6.
Analyst ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31464300

RESUMO

We report here a highly sensitive sandwich type electrochemical aptasensor for lysozyme (lys) detection by the integration of an antifouling interface with HRP-based signal amplification. The biosensing interface with antifouling ability is designed, consisting of a lys-binding aptamer (LBA), dithiothreitol (DTT) and mercaptohexanol (MCH). When lys is captured by the immobilized LBA due to the specific recognition of the aptamer, gold nanoparticles (AuNPs) functionalized with HRP and LBA (HRP-AuNP-LBA) are further conjugated to the surface-bound lys, forming a sandwich assay format. HRP catalyzes the chemical oxidation of hydroquinone (HQ) by hydrogen peroxide (H2O2) to produce benzoquinone (BQ) which results in a large electrochemical reduction signal of BQ. Therefore, this reduction signal measured by differential pulse voltammetry (DPV) is used to detect lys. The catalytic behavior of HRP toward the reaction between HQ and H2O2, together with the high loading of HRP on AuNPs, remarkably amplifies the signal. A linear relationship between the DPV response and the logarithm of lys concentration from 0.01 pg mL-1 to 105 pg mL-1 with a detection limit of 0.003 pg mL-1 (S/N = 3) is obtained. The proposed biosensing platform combines antifouling ability and signal amplification, resulting in high sensitivity, providing an effective way for ultrasensitive assay of protein biomarkers in complex media.

7.
Front Immunol ; 10: 1824, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31428103

RESUMO

Macrophages play a critical role in the pathogenesis of endotoxin shock by producing excessive amounts of pro-inflammatory cytokines. A pan-caspase inhibitor, zVAD, can be used to induce necroptosis under certain stimuli. The role of zVAD in both regulating the survival and activation of macrophages, and the pathogenesis of endotoxin shock remains not entirely clear. Here, we found that treatment of mice with zVAD could significantly reduce mortality and alleviate disease after lipopolysaccharide (LPS) challenge. Notably, in LPS-challenged mice, treatment with zVAD could also reduce the percentage of peritoneal macrophages by promoting necroptosis and inhibiting pro-inflammatory responses in macrophages. In vitro studies showed that pretreatment with zVAD promoted LPS-induced nitric oxide-mediated necroptosis of bone marrow-derived macrophages (BMDMs), leading to reduced pro-inflammatory cytokine secretion. Interestingly, zVAD treatment promoted the accumulation of myeloid-derived suppressor cells (MDSCs) in a mouse model of endotoxin shock, and this process inhibited LPS-induced pro-inflammatory responses in macrophages. Based on these findings, we conclude that treatment with zVAD alleviates LPS-induced endotoxic shock by inducing macrophage necroptosis and promoting MDSC-mediated inhibition of macrophage activation. Thus, this study provides insights into the effects of zVAD treatment in inflammatory diseases, especially endotoxic shock.

8.
ACS Nano ; 13(8): 8760-8765, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31374166

RESUMO

A material with reversible temperature change capability under an external electric field, known as the electrocaloric effect (ECE), has long been considered as a promising solid-state cooling solution. However, electrocaloric (EC) performance of EC materials generally is not sufficiently high for real cooling applications. As a result, exploring EC materials with high performance is of great interest and importance. Here, we report on the ECE of ferroelectric materials with van der Waals layered structure (CuInP2S6 or CIPS in this work in particular). Over 60% polarization charge change is observed within a temperature change of only 10 K at Curie temperature. Large adiabatic temperature change (|ΔT|) of 3.3 K and isothermal entropy change (|ΔS|) of 5.8 J kg-1 K-1 at |ΔE| = 142.0 kV cm-1 and at 315 K (above and near room temperature) are achieved, with a large EC strength (|ΔT|/|ΔE|) of 29.5 mK cm kV-1. The ECE of CIPS is also investigated theoretically by numerical simulation, and a further EC performance projection is provided.

9.
Eur J Obstet Gynecol Reprod Biol ; 240: 336-340, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31382146

RESUMO

OBJECTIVE: ToRCH infections (toxoplasmosis, rubella, cytomegalovirus and Herpes simplex virus) have long been known to be associated with bad obstetric outcomes. Little information is available about the impact of ToRCH infection on reproduction in china nearly for ten years. We designed a prospective study among 1863 pregnant women to investigate the association of ToRCH infection and congenital malformations. STUDY DESIGN: All participants had set up a maternal health Handbook and were managed through the maternal and child health care system. They underwent regular pregnancy check-up, including physical measurements (weight, abdominal circumference and blood pressure), laboratory examinations (blood, urine) and ultrasound scan. ToRCH IgM antibodies were tested by chemiluminescence immunoassay. RESULTS: 102 participants were infected with ToRCH and the total infection rate was 6.06% (102/1683). CMV infection rate (3.15%, 53/1683) was the highest. The positive rate of ToRCH IgM antibodies increased significantly in participant with upper respiratory tract infection (14.6%, 32/219) or with adverse pregnancy history (4.8%, 70/1464). Among 85 ToRCH infected participants, adverse pregnancy outcome were observed in 57 cases which included abortions (31.8%, 27/85), premature births (8.2%, 7/85), congenital malformations (12.9%, 11/85), and stillbirths (9.4%, 8/85). Furthermore, congenital malformations was much higher than that in those without ToRCH infection (1.1%, 17/1598) (P<0.001). CONCLUSION: ToRCH infection was a significant risk factor of severe damage to the fetus, especially congenital malformations. ToRCH screening for pregnant women can reduce the incidence of adverse pregnancy and prevent birth defects in china.

10.
Biosens Bioelectron ; 142: 111535, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31376715

RESUMO

Photoelectrochemical (PEC) sensing has emerged as a simple and practical method for the analysis and detection, its separated optical signal and detection electrical signal give it the advantages of reduced background noise and outstanding sensitivity. Here, we synthesized a Ti3C2/Cu2O composite through simple oil bath heating process, whose excellent PEC performance and sensitive photoelectric response to glucose make it a propitious substitution to glucose oxidase. On this basis, we construct a PEC non-enzymatic sensor based on the Ti3C2/Cu2O heterostructure for the detection of glucose. Under the optimal conditions, the photocurrent of Ti3C2/Cu2O is linear with the logarithm value of glucose concentration in the wide range of 0.5 nM to 0.5 mM with a detection limit of 0.17 nM. Furthermore, the successful detection of glucose in standard samples and human serum by the proposed Ti3C2/Cu2O based PEC non-enzymatic sensor demonstrates the application prospect of heterostructure material in PEC sensor, which provides a new thought for the design and construction of PEC non-enzymatic sensing platform.

11.
Environ Int ; 132: 105092, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31442740

RESUMO

Mulching can effectively maintain and improve soil health and functionality. The mechanisms, however, have not been fully elucidated. Therefore, the effects of temperature on the biogeochemical properties of soil were investigated in the present study in relation to nitrogen management and soil functionality. The results of the 3-year field experiments showed that integrated straw mulch (S) and nitrogen fertilizer (N) treatments enhanced the activities of soil urease, invertase, alkaline phosphatase, and catalase by >1.8, 2.1, 2.0 and 1.4 fold, respectively, compared with the control treatment. Furthermore, these treatments increased soil available N by 28%, phosphorus by 45%, and potassium by 55%. In general, the soil organic carbon, dissolved carbon and labile organic carbon content in the treated plot were approximately 1.2-2.9 folds greater than in the control plot. These improvements in soil fertility and carbon indices increased the biomass and grain yield of soybean (67 and 75%, respectively) during the three-year study period. During 2015-2017, the straw mulch and nitrogen fertilizer treatment increased the soil moisture (23%) and decreased the soil temperature (8%) in comparison to the control in the 0-0.2 m soil depth and, therefore, improved soil enzyme activities, nutrient availability, and carbon stocks, and ultimately, soil functionality and sustainability, in the semiarid region.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31453680

RESUMO

Semiconducting two-dimensional (2D) transition-metal dichalcogenides (TMDCs) are considered promising sensing materials due to the high surface-to-volume ratio and active sensing sites. However, the reported strategies for 2D TMDCs toward sensing of volatile organic compounds (VOCs) present with some drawbacks. These include high operation temperatures, low gas response, and complex fabrication, limiting the development of room-temperature gas sensors. In this study, 2D MoS2 nanoflakes were prepared by liquid-phase exfoliation, and their surface was functionalized with Au nanoparticles (NPs) through a facile solution mixing method. MoS2 decorated with Au NPs with an average size of 10 nm was used as a material platform for an electrochemical sensor to detect a wide variety of VOCs at room temperature. Through dynamic sensing tests, the enhancement of gas-sensing performance in terms of response and selectivity, especially in detecting oxygen-based VOCs (acetone, ethanol, and 2-propanol), was demonstrated. After Au functionalization, the response of the gas sensor to acetone improved by 131% (changing from 13.7% for pristine MoS2 to 31.6% for MoS2-Au(0.5)). Sensing tests under various relative humidity values (10-80%), bending or long-term conditions, indicated the sound robustness and flexibility of the sensor. Density functional theory simulations suggested that the adsorption energy of VOC molecules on MoS2-Au is significantly higher than that on pristine MoS2, contributing to the gas-sensing enhancement; a VOC-sensing mechanism for Au-decorated MoS2 nanoflakes was proposed for the first time for the highly sensitive and selective detection of oxygen-based VOCs.

13.
Med Sci Monit ; 25: 5856-5863, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31386649

RESUMO

BACKGROUND Physical tests are usually preferred to assess rotator-cuff syndrome but are insufficient to predict the morphology and size of the rotator-cuff. The objective of the study was to rate the ultrasound findings for patients with sudden shoulder pain and to determine potential predictors of the same. MATERIAL AND METHODS A total of 112 patients with sudden shoulder pain with rotator-cuff syndrome, suspected by orthopedic doctors, were subjected to ultrasonography. Real-time ultrasonography was done for the acromioclavicular joint, biceps, infraspinatus, posterior labrum, subscapularis, supraspinatus, teres minor tendon, and the sub-acromial-subdeltoid bursa. Each tendon was assessed via scanning planes in orientation as per longer and shorter axis, and from their myotendinous junction shoulder to bony insertions. Linear and logistic regression analysis were performed to predict the associations of medical history with rotator-cuff injury. RESULTS Ultrasonography identified that 82% of the enrolled patients had at least one particular cause of the rotator-cuff disorder. Among the rotator-cuff disorders, calcific tendonitis (54%) was observed more frequently followed by tendinopathy (32%), subacromial-subdeltoid bursitis (22%), and partial thickness tear (21%). Also, 46 patients (41%) had multiple findings. Older age (older than 40 years) was a strong predicting factor of rotator-cuff disorder (r²=0.36, P=0.0004). CONCLUSIONS Ultrasonography is a vital diagnostic procedure used by orthopedic surgeons for diagnosis of the rotator-cuff disorder(s) in patients with sudden shoulder pain.

14.
Parasite ; 26: 53, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31448999

RESUMO

To study the presence of Giardia duodenalis in Xinjiang, northwest China, we collected 801 fecal specimens from seven large-scale pig farms and screened them using PCR targeting the SSU rRNA gene. Twenty-one (2.6%) of the specimens from five farms were G. duodenalis-positive, with a significant difference in prevalence among different farms (0-8.7%) (p < 0.01). Giardia duodenalis prevalence was highest in fattening pigs (5.4%, 7/129), followed by sows (3.2%, 7/222), post-weaning piglets (1.8%, 5/281), and pre-weaning piglets (1.2%, 2/169), but there was no significant difference in prevalence among the age groups (p > 0.05). Sequence analysis of the SSU rRNA gene revealed that the 21 G. duodenalis strains belonged to three assemblages: A (n = 2), B (n = 16), and E (n = 3). Assemblage B was the predominant assemblage and was widely distributed in all G. duodenalis-positive farms and age groups. All G. duodenalis-positive specimens were further assayed at the ß-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes, and two tpi, four gdh, and two bg sequences were identified. These data indicate that pigs may be a zoonotic risk and can potentially spread G. duodenalis infection from animals to humans.

15.
Mol Med Rep ; 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-31432119

RESUMO

Spinal cord injury (SCI) is a specific type of damage to the central nervous system causing temporary or permanent changes in its function. The present aimed to identify the genetic changes in neuroplasticity following SCI in rats. The GSE52763 microarray dataset, which included 15 samples [3 sham (1 week), 4 injury only (1 week), 4 injury only (3 weeks), 4 injury + treadmill (3 weeks)] was downloaded from the Gene Expression Omnibus database. An empirical Bayes linear regression model in limma package was used to identify the differentially expressed genes (DEGs) in injury vs. sham and treadmill vs. non­treadmill comparison groups. Subsequently, time series and enrichment analyses were performed using pheatmap and clusterProfile packages, respectively. Additionally, protein­protein interaction (PPI) and transcription factor (TF)­microRNA (miRNA)­target regulatory networks were constructed using Cytoscape software. In total, 159 and 105 DEGs were identified in injury vs. sham groups and treadmill vs. non­treadmill groups, respectively. There were 40 genes in cluster 1 that presented increased expression levels in the injury (1 week/3 weeks) groups compared with the sham group, and decreased expression levels in the injury + treadmill group compared with the injury only groups; conversely, 52 genes in cluster 2 exhibited decreased expression levels in the injury (1 week/3 weeks) groups compared with the sham group, and increased expression levels in the injury + treadmill group compared with the injury only groups. Enrichment analysis indicated that clusters 1 and 2 were associated with immune response and signal transduction, respectively. Furthermore, microtubule associated protein 1B, phosphofurin acidic cluster sorting protein 2 and adenosylhomocysteinase­like 1 exhibited the highest degrees in the regulatory network, and were regulated by miRNAs including miR­34A, miR­34B, miR­34C and miR­449. These miRNAs and their target genes may serve important roles in neuroplasticity following traumatic SCI in rats. Nevertheless, additional in­depth studies are required to confirm these data.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31437348

RESUMO

Rechargeable aqueous zinc-ion batteries have been considered as a promising candidate for next-generation batteries. However, the formation of zinc dendrites are the most severe problems limiting their practical applications. To develop stable zinc metal anodes, a synergistic method is presented that combines the Cu-Zn solid solution interface on a copper mesh skeleton with good zinc affinity and a polyacrylamide electrolyte additive to modify the zinc anode, which can greatly reduce the overpotential of the zinc nucleation and increase the stability of zinc deposition. The as-prepared zinc anodes show a dendrite-free plating/stripping behavior over a wide range of current densities. The symmetric cell using this dendrite-free anode can be cycled for more than 280 h with a very low voltage hysteresis (93.1 mV) at a discharge depth of 80 %. The high capacity retention and low polarization are also realized in Zn/MnO2 full cells.

17.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(3): 232-238, 2019 May 28.
Artigo em Chinês | MEDLINE | ID: mdl-31257805

RESUMO

OBJECTIVE: To quantitatively investigate the effects of Ringer's solution with different concentrations of alcohol (1%~80%) on biphasic compound action potentials (AP) from frog sciatic nerve trunk, and their recoveries from alcohol effects. METHODS: Individual segments of frog sciatic nerve trunk with a length of 6 to 8 cm were prepared. Ringer's solution with different concentrations of alcohol (0%, 1%, 2%, 4%, 8%, 16%, 32%, 48%, 64% and 80%) was applied onto the segment of the trunk between the stimulus and ground electrodes via an agent reservoir which was newly armed in a nerve trunk shielded chamber for 5 minutes. The nerve trunk was respectively electro-stimulated to generate the biphasic compound AP which was recorded using the experimental system of BL-420F. This was followed by 5 times washout plus 5 min administration with Ringer's solution before recovery recording of AP. RESULTS: Compared to normal Ringer's solution, Ringer's solution with alcohol at ≤4% did not have dramatic impacts on the AP amplitude and conduction velocity, while Ringer's solution with alcohol at ≥8% there was significant decrease in these two parameters. Ringer's solution with alcohol at the conentrations of 16%, 32% and ≥48% could prevent a small proportion (30%), a large proportion (90%) and all (100%) of sciatic nerve trunks, respectively, from generating AP. Washout with normal Ringer's solution after alcohol application at the concentration of ≤32%, AP could totally recover to normal status. While alcohol at the concentration of 48%, 64% and 80%, the probabilities to regenerate APs were 90%, 40% and 0%, and the AP amplitudes were decreased to 60%, 36% and 0%, respectively. After washout, AP conduction velocity showed no difference with alcohol at the concentration of ≤8% when compared with that before washout, while it could not be recovered to normal under alcohol at ≥16%. CONCLUSION: Ringer's solution with different concentrations of alcohol exerts different effects on biphasic compound AP amplitude and conduction velocity. Hopefully, our findings could be helpful for the alcoholic usage and its recovery from alcoholic damage.


Assuntos
Potenciais de Ação , Anuros , Etanol/farmacologia , Solução de Ringer/farmacologia , Nervo Isquiático/efeitos dos fármacos , Animais
18.
J Environ Sci (China) ; 84: 133-143, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31284905

RESUMO

In this study, raw Arundo donax (A. donax) pieces were applied as carbon source and biofilm carriers for denitrification in a lab-scale moving bed biofilm reactor (MBBR) for the treatment of reverse osmosis concentrate gathered from local wastewater reuse plant. At stable phase (about 60 days), efficient denitrification performance was obtained with 73.2% ±â€¯19.5% NO3--N average removal and 8.10 ±â€¯3.45 g N/(m3·day) NO3--N average volumetric removal rate. Mass balance analysis showed that 4.84 g A. donax was required to remove 1 g TN. Quantitative real-time PCR analysis results showed that the copy numbers of 16S r-RNA, narG, nirS, nosZ and anammox gene of carrier biofilm and suspended activated sludge in the declination phase (BF2 and AS2) were lower than those of samples in the stable phase (BF1 and AS1), and relatively higher copy numbers of nirS and nirK genes with lower abundance of narG and nosZ genes were observed. High-throughput sequencing analysis was conducted for BF2 and AS2, and similar dominant phyla and classes with different abundance were obtained. The class Gammaproteobacteria affiliated with the phylum Proteobacteria was the most dominant microbial community in both BF2 (52.6%) and AS2 (41.7%). The PICRUSt prediction results indicated that 33 predictive specific genes were related to denitrification process, and the relative abundance of 18 predictive specific genes in BF2 were higher than those in AS2.


Assuntos
Reatores Biológicos/microbiologia , Desnitrificação , Consórcios Microbianos , Nitrogênio/isolamento & purificação , Poaceae/química , Poaceae/microbiologia , Purificação da Água
19.
ACS Appl Mater Interfaces ; 11(31): 27658-27666, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31290647

RESUMO

Si-based anode materials have attracted considerable attention because of their ultrahigh reversible capacity. However, poor cycling stability caused by the large volume change during cycling prevented the commercial application of Si anodes for lithium-ion batteries (LIBs). To overcome these challenges, in the present study, we designed a nitrogen plasma-treated core-bishell nanostructure where the Si nanoparticle was encapsulated into a SiOx shell and N-doped TiO2-δ shell. Here, the SiOx inside the shell and the TiO2 outside the shell act as binary buffer matrices to accommodate the large volume change and also help to stabilize the solid electrolyte interphase films on the shell surface. More importantly, the plasma-induced N-doped TiO2-δ shell with many Ti3+ species and oxygen vacancies plays a key role in improving the electrical conductivity of Si anodes. Owing to the synergistic effects of SiOx and N-doped TiO2-δ bishells, the cycling stability and rate performance of Si anodes are significantly enhanced. The as-obtained sample exhibits superior cycling stability with a capacity retention of 650 mA h g-1 at 200 mA g-1 after 300 cycles. This strategy is favorable for improving the electrochemical performances of Si-based anodes to employ in practical LIBs.

20.
ACS Appl Mater Interfaces ; 11(29): 26261-26267, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31283165

RESUMO

Single-phase multiferroics are scarce because of the fact that the coexistence of magnetism (spin order) and ferroelectricity (electric dipole order) in a single-phase material may be limited. Taking advantage of the nanocomposite design, combining a ferroelectric phase and a ferromagnetic phase presents enormous opportunities in multiferroic material exploration. In this work, a new 2D-layered framework of Bi2W1-xMnxO6-BiMnO3 (BWMO-BMO) in the nanocomposite thin-film form has been demonstrated and shows obvious room-temperature multiferroic properties, that is, ferroelectric and ferromagnetic at room temperature. The BMO phase forms a unique tilted domain structure in the BWMO matrix, and both phases are of excellent epitaxial quality. The ferroelectric response originates from the layered Aurivillius phase of the BWMO matrix, and the ferromagnetic properties mainly arise from the BMO nanodomains. Moreover, the band gap of the BWMO-BMO nanocomposite is effectively tuned to 3.10 eV from its original 3.75 eV of BWO. This study demonstrates a new design of nanocomposite using layered oxides toward future multifunctional oxides for nanoscale devices.

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