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1.
Food Chem ; 304: 125446, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31491715

RESUMO

Fused coumarins recently attracted strong scientific interest due to their potent pharmacological activities. In this study, density functional theory (DFT) calculations were performed to evaluate the antiradical activities of a series of coumarin-fused coumarins. By calculating the thermodynamic parameters, three primary mechanisms including hydrogen atom transfer (HAT), electron transfer followed by proton transfer (SET-PT) and sequential proton loss electron transfer (SPLET) were examined. It was found that in the gas and benzene phases, the studied compounds prefer to undergo HAT mechanism, while SPLET is more favored in polar media. The results also reveal the possibility of double HAT and double SPLET mechanisms for compound CC-6. Interestingly, a new polycyclic compound was generated by forming a new C5-O5' bond during the second HAT process at the 5'-OH in CC-6-R6 radical. In addition, the SPLHAT mechanism is proposed as a competitive pathway for radical scavenging by CC-4, CC-5 and CC-6.

2.
Sensors (Basel) ; 19(21)2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689926

RESUMO

This paper investigates outage probability (OP) performance predictions using transmit antenna selection (TAS) and derives exact closed-form OP expressions for a TAS scheme. It uses Monte-Carlo simulations to evaluate OP performance and verify the analysis. A back-propagation (BP) neural network-based OP performance prediction algorithm is proposed and compared with extreme learning machine (ELM), locally weighted linear regression (LWLR), support vector machine (SVM), and BP neural network methods. The proposed method was found to have higher OP performance prediction results than the other prediction methods.

3.
Psychiatr Serv ; : appips201900272, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31690223

RESUMO

The goal of this study was to evaluate treatment practices among psychiatry residents before and 3 months after a course on tobacco use disorder. After completing the course, residents (N=89) reported a significant increase in the frequency in nine of the 12 tobacco use disorder treatment practices studied. Participants reported being more likely to assess patient willingness to quit smoking, advise patients to stop smoking, prescribe treatment medications, and provide cessation treatment. Being a junior vs. senior resident was a significant predictor of increased practices. Completing online training was associated with significant increases in the use of specific tobacco treatments.

4.
J Vet Pharmacol Ther ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31691990

RESUMO

The previously adopted marker residue for florfenicol (FF) in China was only florfenicol amine (FFA); however, the marker residue has been changed to FF plus FFA since the end of 2017. The previous official withdrawal period determined based on the only concentration of FFA may no longer be suitable. Therefore, the present study aimed to determine the depletion profiles of FF and FFA and further calculate the withdrawal period in the crucian carp (Carassius auratus) based on the new marker residues. Florfenicol was intramuscularly administered at 10 mg/kg bodyweight daily for five consecutive days to crucian carps reared in freshwater at 10°C. After the last dose, plasma and tissue samples were randomly collected from 10 fish at different time points. The FF and FFA concentrations were simultaneously determined by high-performance liquid chromatography (HPLC) with a fluorescence detector and further subjected to noncompartmental analysis. The elimination half-life (h) of FF in different tissues decreased as follows: liver (39.1) > kidney (36.3) > skin plus muscle (34.6) > plasma (31.7), whereas that of FFA decreased as follows: kidney (41.4) > skin plus muscle (39.4) > liver (39.3) > plasma (35.7). Considering a maximum residue limit of 1 µg/g for the total concentration of FF and FFA in the skin plus muscle, a withdrawal period of 6 days was calculated based on the upper limit of the one-sided 95% confidence interval.

5.
Front Immunol ; 10: 2424, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31708914

RESUMO

Zika virus (ZIKV) specific neutralizing antibodies hold great promise for antibody-based interventions and vaccine design against ZIKV infection. However, their development in infected patients remains unclear. Here, we applied next-generation sequencing (NGS) to probe the dynamic development of a potent and protective ZIKV E DIII-specific antibody ZK2B10 isolated from a ZIKV convalescent individual. The unbiased repertoire analysis showed dramatic changes in the usage of antibody variable region germline genes. However, lineage tracing of ZK2B10 revealed limited somatic hypermutation and transient expansion during the 12 months following the onset of symptoms. The NGS-derived, germline-like ZK2B10 somatic variants neutralized ZIKV potently and protected mice from lethal challenge of ZIKV without detectable cross-reactivity with Dengue virus (DENV). Site-directed mutagenesis identified two residues within the λ chain, N31 and S91, that are essential to the functional maturation of ZK2B10. The repertoire and lineage features unveiled here will help elucidate the developmental process and protective potential of E DIII-directed antibodies against ZIKV infection.

6.
J Crit Care ; 55: 128-133, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31715530

RESUMO

PURPOSE: To assess temporal trends in pre-existing opioid exposure prior to hospitalization among elderly intensive care unit (ICU) patients and its association with adverse outcomes. MATERIALS AND METHODS: We performed a population-based retrospective cohort study using health administrative data from the province of Ontario, Canada. We included all older adult (> 65 years) admissions to an ICU between April 2002 and March 2015. The exposure was opioid use before admission categorized as chronic use, intermittent use, and non-use. RESULTS: The cohort included 711,312 elderly patient admissions to an ICU. Of these, 6.8% (n = 48,363) were chronic opioid users, 28.1% (n = 200,149) intermittent users, and 65.0% (n = 462,800) non-users. Compared with non-users, chronic opioid users and intermittent users had higher in-hospital mortality (adjusted odds ratio: 1.12, 95% CI, 1.09-1.15, p < 0.0001 for chronic users; adjusted odds ratio: 1.09, 95% CI, 1.07-1.11, p < 0.0001 for intermittent users), and a lower subdistribution hazard of time to hospital discharge, translating to a longer hospital length of stay (adjusted hazard ratio: 0.87, 95% CI, 0.85-0.88, p < 0.0001 for chronic users; adjusted hazard ratio: 0.93, 95% CI, 0.92-0.94, p < 0.0001 for intermittent users). CONCLUSIONS: Among elderly ICU patients, opioid exposure prior to admission is prevalent and use is associated with higher in-hospital mortality.

7.
Bioresour Technol ; 296: 122276, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31677406

RESUMO

Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1-7 g NH4+-N L-1). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.

8.
Sci Rep ; 9(1): 16697, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31723171

RESUMO

Although largemouth bass Micropterus salmoides has shown its extremely economic, ecological, and aquacultural significances throughout the North American and Asian continents, systematic evaluation of genetic variation and structure of wild and cultured populations of the species is yet to be documented. In this study, we investigated the genetic structure of M. salmoides from 20 wild populations and five cultured stocks across the United States and China using eight microsatellite loci, which are standard genetic markers for population genetic analysis. Our major findings are as follows: (1) the result of Fst showed largemouth bass had high genetic differentiation, and the gene flow indicated the genetic exchange among wild populations is difficult; (2) AMOVA showed that 14.05% of the variation was among populations, and 85.95% of the variation was within populations; (3) The majority of largemouth bass populations had a significant heterozygosity excess, which is likely to indicate a previous population bottleneck; (4) Allelic richness was lower among cultured populations than among wild populations; (5) Effective population size in hatcheries could promote high levels of genetic variation among individuals and minimize loss of genetic diversity; China's largemouth bass originated from northern largemouth bass of USA. The information provides valuable basis for development of appropriate conservation policies for fisheries and aquaculture genetic breeding programs in largemouth bass.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31701734

RESUMO

Silicon integration of nanoscale metamaterials is a crucial step toward low-cost and scalable optical-based integrated circuits. Here, a self-assembled epitaxial Au-BaTiO3 (Au-BTO) hybrid metamaterial with highly anisotropic optical properties has been demonstrated on Si substrates. A thin buffer layer stack (<20 nm) of TiN and SrTiO3 (STO) was applied on Si substrates to ensure the epitaxial growth of the Au-BTO hybrid films. Detailed phase composition and microstructural analyses show excellent crystallinity and epitaxial quality of the Au-BTO films. By varying the film growth condition, the density and dimension of the Au nanopillars can be tuned effectively, which leads to highly tailorable optical properties including tunable localized surface plasmon resonance (LSPR) peak and hyperbolic dispersion shift in the visible and near-infrared regime. The work highlights the feasibility of integrating epitaxial hybrid oxide-metal plasmonic metamaterials on Si towards future complex Si-based integrated photonics.

10.
Sci Rep ; 9(1): 16595, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31719551

RESUMO

Using two advanced sequencing approaches, Illumina and PacBio, we derive the entire Dscam gene from an M2 assembly of the complete Penaeus monodon genome. The P. monodon Dscam (PmDscam) gene is ~266 kbp, with a total of 44 exons, 5 of which are subject to alternative splicing. PmDscam has a conserved architectural structure consisting of an extracellular region with hypervariable Ig domains, a transmembrane domain, and a cytoplasmic tail. We show that, contrary to a previous report, there are in fact 26, 81 and 26 alternative exons in N-terminal Ig2, N-terminal Ig3 and the entirety of Ig7, respectively. We also identified two alternatively spliced exons in the cytoplasmic tail, with transmembrane domains in exon variants 32.1 and 32.2, and stop codons in exon variants 44.1 and 44.2. This means that alternative splicing is involved in the selection of the stop codon. There are also 7 non-constitutive cytoplasmic tail exons that can either be included or skipped. Alternative splicing and the non-constitutive exons together produce more than 21 million isoform combinations from one PmDscam locus in the P. monodon gene. A public-facing database that allows BLAST searches of all 175 exons in the PmDscam gene has been established at http://pmdscam.dbbs.ncku.edu.tw/ .

11.
Mol Plant Pathol ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31721403

RESUMO

Citrus huanglongbing (HLB) is the most devastating citrus disease worldwide. 'Candidatus Liberibacter asiaticus' (Las) is the most prevalent HLB causal agent that is yet to be cultured. Here, we analysed the flagellar genes of Las and Rhizobiaceae and observed two characteristics unique to the flagellar proteins of Las: (i) a shorter primary structure of the rod capping protein FlgJ than other Rhizobiaceae bacteria and (ii) Las contains only one flagellin-encoding gene flaA (CLIBASIA_02090), whereas other Rhizobiaceae species carry at least three flagellin-encoding genes. Only flgJAtu but not flgJLas restored the swimming motility of Agrobacterium tumefaciens flgJ mutant. Pull-down assays demonstrated that FlgJLas interacts with FlgB but not with FliE. Ectopic expression of flaALas in A. tumefaciens mutants restored the swimming motility of ∆flaA mutant and ∆flaAD mutant, but not that of the null mutant ∆flaABCD. No flagellum was observed for Las in citrus and dodder. The expression of flagellar genes was higher in psyllids than in planta. In addition, western blotting using flagellin-specific antibody indicates that Las expresses flagellin protein in psyllids, but not in planta. The flagellar features of Las in planta suggest that Las movement in the phloem is not mediated by flagella. We also characterized the movement of Las after psyllid transmission into young flush. Our data support a model that Las remains inside young flush after psyllid transmission and before the flush matures. The delayed movement of Las out of young flush after psyllid transmission provides opportunities for targeted treatment of young flush for HLB control.

12.
Carbohydr Polym ; 226: 115304, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31582069

RESUMO

Medicinal herb Bletilla striata as a traditional Chinese herb has been used to treat alimentary canal mucosal damage, ulcers, bruises, and burns for thousands of years. Despite numerous efforts directed at the development of Bletilla striata products, the challenge of preparing Bletilla striata hemostasis dressings while simultaneously maintaining portability and high hemostasis performance has not yet been addressed. Herein, we describe a Bletilla striata polysaccharide (BSP) sponge with hierarchical aligned porous channels formed via directional freeze technology. The microstructure and mechanical property of the BSP sponges could be controlled by modifying the BSP concentration. Notably, in vivo animal studies indicated that BSP sponges show high biocompatibility and degradation and possess excellent hemostasis capability. In addition, BSP sponges showed accelerated wound healing in comparison to commercial dressings. Thus, our study indicates that the constructed BSP sponges could find potential application in dressings for efficient wound healing.

13.
J Chem Phys ; 151(12): 124106, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31575206

RESUMO

UV pump-XUV/X-ray probe measurements have been successfully applied in the study of photo-induced chemical reactions. Although rich element-specific electronic structure information is accessible within XUV/X-ray (inner-shell) absorption spectra, it can be difficult to interpret the chemistry directly from the spectrum without supporting theoretical simulations. A multireference method to completely simulate UV pump-XUV/X-ray probe measurement has been developed and applied to study the methyl iodide photodissociation process. Multireference, fewest-switches surface hopping (FSSH) trajectories were used to explore the coupled electronic and ionic dynamics upon photoexcitation of methyl iodide. Interpretation of previous measurements is provided by associated multireference, restricted active space, inner-shell spectral simulations. This combination of multireference FSSH trajectories and XUV spectra provides an interpretation of transient features appearing in previous measurements within the first 100 fs after photoexcitation and validates the significant branching ratio in the final excited-state population. This methodology should prove useful for interpretation of the increasing number of inner-shell probe studies of molecular excited states or for directing new experiments toward interesting regions of the potential energy landscape.

14.
Mol Med Rep ; 20(6): 4883-4892, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638166

RESUMO

The prevalence of immune­mediated liver diseases such as autoimmune liver disease or viral hepatitis has increased in recent years, and the side effects of pre­existing treatments are a worldwide problem. Regulatory T cells (Tregs) and T helper 17 (Th17) cells play important roles in the development of immune­mediated hepatitis and may serve as potential therapeutic targets. Tofacitinib, a new Janus kinase (JAK) inhibitor, is under investigation for the treatment of rheumatoid arthritis; it is also helpful in treating ulcerative colitis and psoriasis. The roles of tofacitinib were investigated in conferring protection against immune­mediated liver injury in mice. T cell­mediated hepatitis was induced by concanavalin A (ConA). The mice in the treatment groups were administered with tofacitinib intragastrically before the ConA injection. Histopathological examination was performed by hematoxylin and eosin (H&E) staining, and the serum transaminase and inflammatory cytokine levels were determined using an automatic biochemistry analysis apparatus or cytometric bead array (CBA) kits. Flow cytometric analysis was used to detect Tregs and Th17 cells. Tofacitinib significantly decreased the hepatic injury induced by ConA and prominently decreased the liver transaminase level. The secretion of several anti­inflammatory cytokines such as interleukin (IL)­10 was upregulated in mice from the treatment group, compared to that in mice treated with ConA alone, while the expression of interferon­Î³ (IFN­Î³) and tumor necrosis factor­α (TNF­α) decreased. Tofacitinib treatment increased the number of Tregs and reduced the number of Th17 cells. Furthermore, tofacitinib could relieve liver fibrosis under conditions of autoimmune hepatitis (AIH). The present results indicated that tofacitinib improved immune­mediated hepatitis and restored the impaired Treg/Th17 cell ratio, which suggests that it may serve as a novel treatment approach for immune­mediated liver diseases.

15.
Oncol Rep ; 42(6): 2572-2582, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638237

RESUMO

Long noncoding RNAs (lncRNAs) have been confirmed to be potential prognostic markers in a variety of cancers and to interact with microRNAs (miRNAs) as competing endogenous RNAs (ceRNAs) to regulate target gene expression. However, the role of lncRNA­mediated ceRNAs in breast cancer (BC) remains unclear. In the present study, a ceRNA network was generated to explore their role in BC. The expression profiles of mRNAs, miRNAs and lncRNAs in 1,109 BC tissues and 113 normal breast tissues were obtained from The Cancer Genome Atlas database (TCGA). A total of 3,198 differentially expressed (DE) mRNAs, 150 differentially DEmiRNAs and 1,043 DElncRNAs were identified between BC and normal tissues. A lncRNA­miRNA­mRNA network associated with BC was successfully constructed based on the combined data obtained from RNA databases, and comprised 97 lncRNA nodes, 24 miRNA nodes and 74 mRNA nodes. The biological functions of the 74 DEmRNAs were further investigated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The results demonstrated that the DEmRNAs were significantly enriched in two GO biological process categories; the main biological process enriched term was 'positive regulation of GTPase activity'. By KEGG analysis, four key enriched pathways were obtained, including the 'MAPK signaling pathway', the 'Ras signaling pathway', 'prostate cancer', and the 'FoxO signaling pathway'. Kaplan­Meier survival analysis revealed that six DElncRNAs (INC AC112721.1, LINC00536, MIR7­3HG, ADAMTS9­AS1, AL356479.1 and LINC00466), nine DEmRNAs (KPNA2, RACGAP1, SHCBP1, ZNF367, NTRK2, ORS1, PTGS2, RASGRP1 and SFRP1) and two DEmiRNAs (hsa­miR­301b and hsa­miR­204) had significant effects on overall survival in BC. The present results demonstrated the aberrant expression of INC AC112721.1, AL356479.1, LINC00466 and MIR7­3HG in BC, indicating their potential prognostic role in patients with BC.

16.
Mol Med Rep ; 20(6): 4843-4854, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31661144

RESUMO

MicroRNAs serve an important role in the development of several diseases. Numerous genes regulate the skeletal muscle differentiation of C2C12 myoblasts. The role of miR­760 in rheumatoid arthritis (RA) has not been reported, to the best of our knowledge. Therefore, the aim of the present study was to examine the role of miR­760 in regulating skeletal muscle proliferation in RA. Potential genes functionally involved in the tarsal joint of a collagen­induced RA model were identified using Gene Expression Omnibus. Reverse transcription­quantitative PCR and western blot analyses were performed to determine the mRNA and protein expression levels. The proliferation, cell cycle progression and migration of C2C12 myoblasts were detected using Cell Counting Kit­8, flow cytometry and wound­healing assays, respectively. TargetScan was used to predict the potential target genes of miR­760, and this was verified using a dual­luciferase reporter assay. In the present study, myosin­18b (Myo18b) expression was determined to be downregulated in the RA model. Silencing Myo18b decreased the proliferation, abrogated the cell cycle progression, and reduced the migration and differentiation of C2C12 myoblasts. Expression levels of cyclin­dependent kinase 2, cyclin D1, matrix metalloproteinase (MMP)­2, MMP­9, myogenin and myosin heavy chain 6 were all decreased when Myo18b was silenced. Furthermore, overexpression of Myo18b induced opposing effects on C2C12 myoblasts. It was shown that Myo18b was a target gene of miRNA­760. Overexpression of miR­760 decreased proliferation, cell cycle progression, migration and differentiation in C2C12 myoblasts, and decreased the expression of Myo18b. The opposite results were observed when miR­760 was downregulated. In conclusion, miR­760 inhibited proliferation and differentiation by targeting Myo18b in C2C12 myoblasts. The results of the present study may contribute to understanding the mechanisms underlying RA skeletal muscle proliferation, and miR­760/Myo18b may serve as potential targets for treating patients with RA.

17.
Ann Vasc Surg ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31655109

RESUMO

BACKGROUND: Neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) are two markers of inflammation, which are associated with worse cardiovascular disease outcomes. Here we aimed to determine the association between these ratios and disease severity, and evaulate predictive validity of NLR and PLR in lower limb arteriosclerosis obliterans (ASO). METHODS: We evaluated 211 patients with a diagnosis of ASO from January 2016 to December 2018 at Shanghai Jiaotong University Renji hospital. NLR and PLR were accessed from routinely drawn peripheral venous blood at the ward of vascular surgery during hospitalization. the association between NLR and PLR with baseline characteristics, disease severity, one-year outcomes were determined, respectively. RESULTS: Both NLR and PLR showed significant values on predicting disease severity. Higher NLR(p=0.001) and PLR(p<0.001) were associated with lower ABI index and worse clinical presentation. Both NLR and PLR are positively correlated with one-year readmission rate (p<0.001, p=0.001, respectively). Both NLR and PLR also positively correlated with tissue loss rate and one-year mortality (p=0.007, p=0.034, respectively). CONCLUSION: NLR and PLR show a positive association with the severity of lower extremity PAD, both higher ratios correlate with poor prognosis, especially, the risk of one-year readmission. A higher NLR also correlates with one-year mortality.

18.
Microb Cell Fact ; 18(1): 169, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601211

RESUMO

BACKGROUND: With a variety of physiological and pharmacological functions, menaquinone is an essential prenylated product that can be endogenously converted from phylloquinone (VK1) or menadione (VK3) via the expression of Homo sapiens UBIAD1 (HsUBIAD1). The methylotrophic yeast, Pichia pastoris, is an attractive expression system that has been successfully applied to the efficient expression of heterologous proteins. However, the menaquinone biosynthetic pathway has not been discovered in P. pastoris. RESULTS: Firstly, we constructed a novel synthetic pathway in P. pastoris for the production of menaquinone-4 (MK-4) via heterologous expression of HsUBIAD1. Then, the glyceraldehyde-3-phosphate dehydrogenase constitutive promoter (PGAP) appeared to be mostsuitable for the expression of HsUBIAD1 for various reasons. By optimizing the expression conditions of HsUBIAD1, its yield increased by 4.37 times after incubation at pH 7.0 and 24 °C for 36 h, when compared with that under the initial conditions. We found HsUBIAD1 expressed in recombinant GGU-23 has the ability to catalyze the biosynthesis of MK-4 when using VK1 and VK3 as the isopentenyl acceptor. In addition, we constructed a ribosomal DNA (rDNA)-mediated multi-copy expression vector for the fusion expression of SaGGPPS and PpIDI, and the recombinant GGU-GrIG afforded higher MK-4 production, so that it was selected as the high-yield strain. Finally, the yield of MK-4 was maximized at 0.24 mg/g DCW by improving the GGPP supply when VK3 was the isopentenyl acceptor. CONCLUSIONS: In this study, we constructed a novel synthetic pathway in P. pastoris for the biosynthesis of the high value-added prenylated product MK-4 through heterologous expression of HsUBIAD1 and strengthened accumulation of GGPP. This approach could be further developed and accomplished for the biosynthesis of other prenylated products, which has great significance for theoretical research and industrial application.

19.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3358-3364, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602895

RESUMO

To evaluate the effect of Tripterygium Glycosides Tablets extract in the treatment of rheumatoid arthritis( RA). Clinical trials of treating rheumatoid arthritis with Tripterygium Glycosides Tablets published by Meta-analysis were retrieved from EMbase,PubMed,Clinical Trials,Web of Science,Cochrane Library,CNKI,Wanfang,VIP,CBM and Chi CTR,and comprehensively analyzed. A total of 3 studies were enrolled,the modified Sharp score( m TSS),tender join joint erosions( JE) and joint space narrowing( JSN) of Tripterygium Glycosides Tablets group were significant superior to those of control group,including positive drugs methotrexate( MTX) and salazopyridine( SSZ)( P<0. 01). Tripterygium Glycosides Tablets had an effect in treating RA. Due to the small sample size,this study shall be verified with high-quality,large-sample-size double-blinded RCTs.


Assuntos
Antirreumáticos/farmacologia , Artrite Reumatoide/tratamento farmacológico , Glicosídeos/farmacologia , Tripterygium/química , Humanos , Comprimidos
20.
BMC Plant Biol ; 19(1): 417, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604417

RESUMO

BACKGROUND: The content of stone cells and lignin is one of the key factors affecting the quality of pear fruit. In a previous study, we determined the developmental regularity of stone cells and lignin in 'Dangshan Su' pear fruit 15-145 days after pollination (DAP). However, the development of fruit stone cells and lignin before 15 DAP has not been heavily researched. RESULTS: In this study, we found that primordial stone cells began to appear at 7 DAP and that the fruit had formed a large number of stone cells at 15 DAP. Subsequently, transcriptome sequencing was performed on fruits at 0, 7, and 15 DAP and identified 3834 (0 vs. 7 DAP), 4049 (7 vs. 15 DAP) and 5763 (0 vs. 15 DAP) DEGs. During the 7-15 DAP period, a large number of key enzyme genes essential for lignin biosynthesis are gradually up-regulated, and their expression pattern is consistent with the accumulation of lignin in this period. Further analysis found that the biosynthesis of S-type lignin in 'Dangshan Su' pear does not depend on the catalytic activity of PbSAD but is primarily generated by the catalytic activity of caffeoyl-CoA through CCoAOMT, CCR, F5H, and CAD. We cloned PbCCR1, 2 and analysed their functions in Chinese white pear lignin biosynthesis. PbCCR1 and 2 have a degree of functional redundancy; both demonstrate the ability to participate in lignin biosynthesis. However, PbCCR1 may be the major gene for lignin biosynthesis, while PbCCR2 has little effect on lignin biosynthesis. CONCLUSIONS: Our results revealed that 'Dangshan Su' pear began to form a large number of stone cells and produce lignin after 7 DAP and mainly accumulated materials from 0 to 7 DAP. PbCCR1 is mainly involved in the biosynthesis of lignin in 'Dangshan Su' pear and plays a positive role in lignin biosynthesis.

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