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1.
Org Lett ; 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36662155

RESUMO

The biorelevant sulfur-containing Euphorbia diterpenes with scarce 5/7/6/3 premyrsinane- and 5/7/6 myrsinane-type backbones were easily constructed from naturally abundant lathyrane-type Euphorbia factor L3 by visible-light-triggered tandem thiol-ene click reaction/transannular cyclization and regioselective cyclopropane ring-opening. The selenide diterpene was also successfully obtained to verify the system universality. This concise synthesis route gives an efficient strategy for obtaining structurally diverse Euphorbia diterpenes under very mild conditions and provides a promising anti-HIV bioactive premyrsinane diterpene 3h.

2.
Small ; : e2205957, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36610043

RESUMO

The discovery of carbon-based quantum dots (CQDs) has allowed opportunities for fluorescence bioimaging, tumor diagnosis and treatment, and photo-/electro-catalysis. Nevertheless, in the existing reviews related to the "bottom-up" approaches, attention is mainly paid to the applications of CQDs but not the formation mechanism of CQDs, which mainly derived from the high complexities during the synthesis of CQDs. Among the various synthetic methods, using small molecules as "building blocks", the development of a "bottom-up" approach has promoted the structural design, modulation of the photoluminescence properties, and control of the interfacial properties of CQDs. On the other hand, many works have demonstrated the "building blocks"-dependent properties of CQDs. In this review, from one of the most important variables, the relationships among intrinsic properties of "building blocks" and photoluminescence properties of CQDs are summarized. The topology, chirality, and free radical process are selected as descriptors for the intrinsic properties of "building blocks". This review focuses on the induction and summary of recent research results from the "bottom-up" process. Moreover, several empirical rules pertaining thereto are also proposed.

3.
Cardiology ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36596284

RESUMO

INTRODUCTION: Data on first-line ablation treatment for patients with symptomatic atrial fibrillation (AF) are scarce. This study indirectly compared the efficacy and safety of cryoballoon ablation (CBA) vs. radiofrequency ablation (RFA) as initial therapy for symptomatic AF. METHODS: We searched the EMBASE, PubMed, Cochrane Library, and ClinicalTrials.gov databases for randomized controlled trials (RCTs) that compared CBA or RFA with antiarrhythmic drugs (AADs) as first-line treatment for AF from the time of database establishment up to December 2021. The odds ratio (OR) with a 95% confidence interval (CI) was used as a measure of the treatment effect. RESULTS: Six RCTs (3 CBA, 3 RFA) that enrolled a total of 1215 patients were included in this analysis. There were no significant differences in atrial arrhythmia (AA) (OR 0.993, 95% CI 0.602-1.638), symptomatic AA (OR 0.638, 95% CI 0.344-1.182), or serious adverse events (OR 1.474, 95% CI, 0.404-5.376) between the two ablation techniques. The incidences of additional CBA therapy (OR 2.693, 95% CI 1.277-5.681) and patients who crossed over to AAD therapy (OR 0.345 95% CI 0.179-0.664) in the CBA group were significantly lower than that in the RFA group. CONCLUSION: Among patients with paroxysmal AF receiving initial therapy, CBA and RFA share a similar efficacy and safety profile. When pulmonary vein isolation is performed by CBA, study crossover and the need for additional ablation are substantially lower.

4.
Huan Jing Ke Xue ; 44(1): 169-179, 2023 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-36635805

RESUMO

The widespread use of antibiotics has led to a large number of antibiotics entering the environment, to which microorganisms have become resistant. In recent years, with the intensification of human activities in the plateau region, the occurrence and migration of antibiotic resistance genes (ARGs) in plateau wetlands have attracted considerable attention. Here, we selected the Caohai National Wetland Park, located in the Yunnan-Guizhou Plateau, as our study area. The contents of tetracyclines, sulfonamides, quinolones, and macrolides in sediments from the upstream (the pristine habitat near the spring eye) and downstream (the sewage discharge outlet of residents) areas of the river in the park were analyzed. Among them, the detection content of tetracycline antibiotics was 103.65-2185 µg·kg-1, which was the highest antibiotic detection content. To further investigate the occurrence characteristics and influencing factors of tetracycline resistance genes, the influence of environmental factors, bacterial community structure, and pathogenic bacteria on tetracycline ARGs under the influence of human activities were revealed via correlation analysis and network analysis. The results showed that a total of 15 tetracycline resistance genes were detected in the upstream and downstream sediments. Among them, seven resistance genes including tetPA, tetD, and tetPB were detected in the upstream, and 13 resistance genes such as tetPA, tetE, tetM, and tetX were detected in the downstream. The abundance of eight new resistance genes in the downstream accounted for 43.44% of the downstream genes. The tetracycline-like antibiotics and soil physicochemical indicators (i.e., available phosphorus, total organic carbon, nitrate nitrogen, and total phosphorus) were the main environmental factors affecting the distribution of tetracycline ARGs. Additionally, the bacteria detected in the upstream and downstream sediments belonged to 64 bacterial phyla, among which Proteobacteria, Firmicutes, and Bacteroidota were the main phyla affecting the abundance of tetracycline ARGs; meanwhile, 27 pathogenic bacteria were detected in the upstream and downstream sediments. Network analysis showed that the correlation between the eight new resistance genes and pathogens in the downstream area accounted for 70% of the network connectivity, and Listeria monocytogenes, Enterococcus faecalis, and Bacteroides vulgatus were identified as potential hosts for the transmission of tetracycline ARGs. Compared to the pristine habitat, the discharge of domestic sewage introduced large amounts of antibiotics and also changed the microenvironment and microbial community structure of the river wetland. Additionally, it increased the species of ARGs in sediments, which promoted the spread and transmission of ARGs among microorganisms and even pathogens.


Assuntos
Microbiota , Tetraciclina , Humanos , Tetraciclina/farmacologia , Tetraciclina/análise , Áreas Alagadas , Esgotos/microbiologia , Genes Bacterianos , China , Antibacterianos/farmacologia , Antibacterianos/análise , Resistência Microbiana a Medicamentos/genética , Bactérias/genética , Tetraciclinas/farmacologia , Tetraciclinas/análise , Atividades Humanas
5.
Opt Lett ; 48(2): 299-302, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36638442

RESUMO

We report the 1.3/1.4 µm dual-wave band dissipative soliton resonance (DSR) in a passively mode-locked bismuth-doped phosphosilicate fiber (Bi-PSF) laser. The low-water-peak Bi-PSF with two bismuth active centers associated with silicon and phosphorus supports the O+E-band gain. Using a 1239 nm home-made Raman fiber laser as pump source and nonlinear amplifying loop mirror for initiating mode-locking, stable DSR operation at 1343 and 1406 nm is achieved with the spectral bandwidth of 12 and 16 nm. The pulse duration with the pump power increases from 62 to 270 ps with a repetition frequency of 4.069 MHz. The average power is 11.05 mW corresponding to the maximum energy of 2.7 nJ. This is, to the best of our knowledge, the first demonstration of a mode-locked fiber laser in the ∼1.38 µm water absorption band and the O+E dual-wave band operation for applications in all-spectral-band communications, bio-medical imaging, and terahertz difference frequency generation.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36633752

RESUMO

The effect of ambient temperature on health continues to draw more and more attention with the global warming. Bacillary dysentery (BD) is a major global environmental health issue and affected by temperature and other environmental variables. In the current study, we evaluated the effect of temperature on the incidence of BD from January 1st, 2008 to December 31st, 2011 in Jiayuguan, a temperate continental arid climate city in the Hexi Corridor of northwest China. A distributed lag non-linear model (DLNM) was performed to evaluate the lag effect of temperature on BD up to 30 days. Results showed the risk of BD increased with temperature significantly, especially after 8 °C. The maximum risk of BD was observed at extreme high temperature (29 °C). The effect of temperature on BD risk was significantly divided into short-term effect at lag 5 days and long-term effect at lag 30 days. Age ≤ 15 years were most affected by high temperature. The maximum cumulative risk for lag 30 days (25.8, 95% CIs: 11.8-50.1) was observed at 29 °C. Age ≤ 15 years and females showed short-term effect at lag 5 days and long-term effect at lag 30 days, while age > 15 years and males showed acute short-term effect at lag 0 and light long-term effect at lag 16 days.

7.
Thromb J ; 21(1): 3, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624481

RESUMO

BACKGROUND: Antithrombin (AT) is the main physiological anticoagulant involved in hemostasis. Hereditary AT deficiency is a rare autosomal dominant thrombotic disease mainly caused by mutations in SERPINC1, which was usually manifested as venous thrombosis and pulmonary embolism. In this study, we analyzed the clinical characteristics and screened for mutant genes in two pedigrees with hereditary AT deficiency, and the functional effects of the pathogenic mutations were evaluated. METHODS: Candidate gene variants were analyzed by next-generation sequencing to screen pathogenic mutations in probands, followed by segregation analysis in families by Sanger sequencing. Mutant and wild-type plasmids were constructed and transfected into HEK293T cells to observe protein expression and cellular localization of SERPINC1. The structure and function of the mutations were analyzed by bioinformatic analyses. RESULTS: The proband of pedigree A with AT deficiency carried a heterozygous frameshift mutation c.1377delC (p.Asn460Thrfs*20) in SERPINC1 (NM000488.3), a 1377C base deletion in exon 7 resulting in a backward shift of the open reading frame, with termination after translation of 20 residues, and a different residue sequence translated after the frameshift. Bioinformatics analysis suggests that the missing amino acid sequence caused by the frameshift mutation might disrupt the disulfide bond between Cys279 and Cys462 and affect the structural function of the protein. This newly discovered variant is not currently included in the ClinVar and HGMD databases. p.Arg229* resulted in a premature stop codon in exon 4, and bioinformatics analysis suggests that the truncated protein structure lost its domain of interaction with factor IX (Ala414 site) after the deletion of nonsense mutations. However, considering the AT truncation protein resulting from the p.Arg229* variant loss a great proportion of the molecule, we speculate the variant may affect two functional domains HBS and RCL and lack of the corresponding function. The thrombophilia and decreased-AT-activity phenotypes of the two pedigrees were separated from their genetic variants. After lentiviral plasmid transfection into HEK293T cells, the expression level of AT protein decreased in the constructed c.1377delC mutant cells compared to that in the wild-type, which was not only reduced in c.685C > T mutant cells but also showed a significant band at 35 kDa, suggesting a truncated protein. Immunofluorescence localization showed no significant differences in protein localization before and after the mutation. CONCLUSIONS: The p.Asn460Thrfs*20 and p.Arg229* variants of SERPINC1 were responsible for the two hereditary AT deficiency pedigrees, which led to AT deficiency by different mechanisms. The p.Asn460Thrfs*20 variant is reported for the first time.

8.
Eur J Radiol ; 159: 110665, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36566705

RESUMO

PURPOSE: To determine the prognostic value of tumour contour irregularity degree (CID) in surgical strategy options for T1bN0M0 renal cell carcinoma (RCC). MATERIALS AND METHODS: We performed a retrospective multi-institutional review of 489 patients with T1bN0M0 RCC treated between January 2009 and June 2019. Cox regression and Kaplan-Meier analyses were performed to analyse the impact of CID on disease-free survival (DFS). RESULTS: The median follow-up time was 55 months (interquartile range, 40-81 months) for 55 (11.2 %) patients with metastasis or recurrence. Logistic analysis indicated that CID was associated with World Health Organization/International Society of Urological Pathology (WHO/ISUP) grades III-IV (odds ratio, 1.015; 95 % confidence interval [CI], 1.008-1.023; p < 0.001). After being classified into high CID (≥50 %) and low CID (<50 %) groups, those with a high CID showed a significantly higher ratio of WHO/IUSP grades III-IV (74/277 [26.7 %] vs 25/212 [11.8 %]) and shorter DFS than the low CID group (p < 0.001). Multivariable Cox regression showed that partial nephrectomy (PN; hazard ratio [HR], 1.889; 95 % CI, 1.020-3.499; p = 0.043), high CID (HR, 6.685; 95 % CI, 2.776-16.100; p < 0.001), and WHO/ISUP grade III-IV (HR, 1.950; 95 % CI, 1.100-3.458; p = 0.022) were independent prognostic factors for DFS. The Kaplan-Meier plot showed that PN had a DFS rate comparable to that of radical nephrectomy (RN; p = 0.994). In the low CID group, patients who underwent PN showed comparable DFS to those who underwent RN (p = 0.903). Furthermore, patients with a high CID tended to have worse DFS in the PN versus RN group (p = 0.044). Multivariable Cox regression showed that PN (HR, 2.049; 95 % CI, 1.065-3.942; p = 0.032) and WHO/ISUP grade III-IV (HR, 2.148; 95 % CI, 1.189-3.881; p = 0.011) were independent prognostic factors of DFS in the high CID group. CONCLUSIONS: CID is a reliable preoperative parameter which is positively correlated with WHO/ISUP grade and can help with surgical decision-making in patients with T1bN0M0 RCC.


Assuntos
Carcinoma de Células Renais , Neoplasias Renais , Humanos , Carcinoma de Células Renais/cirurgia , Carcinoma de Células Renais/patologia , Prognóstico , Neoplasias Renais/cirurgia , Neoplasias Renais/patologia , Estudos Retrospectivos , Resultado do Tratamento , Estadiamento de Neoplasias , Nefrectomia
9.
Anal Bioanal Chem ; 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36459169

RESUMO

Fungi with hallucinogenic properties and neurotoxicity have been listed as prohibited drugs in recent years, but there is a lack of in situ quantification of psilocybin and analogues in these samples to avoid the decomposition of these psychoactive tryptamines in time-consuming sample preparation. In this study, matrix-assisted laser-desorption/ionization (MALDI)-Fourier transform ion cyclotron resonance (FT ICR) mass spectrometric imaging (MSI) was used to analyze the distribution of psilocybin and its analogues in hallucinogenic Psilocybe mushrooms. A cesium chloride (CsCl)-coated target plate was prepared to improve the detection sensitivity and reduce the interference of other compounds or decomposition products with very similar m/z values in MALDI-FT ICR MS analysis. Psilocybin and other tryptamines with structurally similar compounds, including psilocin, baeocystin, tryptophan, tryptamine, and aeruginascin, were identified and imaged in the psilocybe tissue section; the semiquantitative analysis of the distribution of psilocybin was also investigated using a homemade 75-well CsCl-coated plate; and the target plate can be placed on the mass spectrometry target carrier along with the indium-tin oxide (ITO) conductive slide, which can simultaneously carry out matrix vapor deposition, thus ensuring the parallelism between the standards and samples in the pretreatment experiment and MSI. The contents of psilocybin and its analogues in the psilocybe tissue section can be evaluated from the color changes corresponding to different concentration standard curves. Furthermore, a comprehensive comparison between MALDI-FT ICR MS and ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-Q/TOF MS) analysis was performed for quantification and validation. This study reduces the decomposition in time-consuming sample pretreatment and provides a powerful tool for drug abuse control and forensic analysis.

10.
Int J Mol Sci ; 23(24)2022 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-36555248

RESUMO

This study evaluated the mechanism of temperature-controlled repeated thermal stimulation (TRTS)-mediated neuronal differentiation. We assessed the effect of SP600125, a c-Jun N-terminal kinase (JNK) inhibitor, on neuronal differentiation of rat PC12-P1F1 cells, which can differentiate into neuron-like cells by exposure to TRTS or neurotrophic factors, including bone morphogenetic protein (BMP) 4. We evaluated neuritogenesis by incubating the cells under conditions of TRTS and/or SP600125. Cotreatment with SP600125 significantly enhanced TRTS-mediated neuritogenesis, whereas that with other selective mitogen-activated protein kinase (MAPK) inhibitors did not-e.g., extracellular signal-regulated kinase (ERK)1/2 inhibitor U0126, and p38 MAPK inhibitor SB203580. We tried to clarify the mechanism of SP600125 action by testing the effect of U0126 and the BMP receptor inhibitor LDN193189 on the SP600125-mediated enhancement of intracellular signaling. SP600125-enhanced TRTS-induced neuritogenesis was significantly inhibited by U0126 or LDN193189. Gene expression analysis revealed that TRTS significantly increased ß3-Tubulin, MKK3, and Smad7 gene expressions. Additionally, Smad6 and Smad7 gene expressions were substantially attenuated through SP600125 co-treatment during TRTS. Therefore, SP600125 may partly enhance TRTS-induced neuritogenesis by attenuating the negative feedback loop of BMP signaling. Further investigation of the mechanisms underlying the effect of SP600125 during TRTS-mediated neuritogenesis may contribute to the future development of regenerative neuromedicine.


Assuntos
Butadienos , Crescimento Neuronal , Animais , Ratos , Butadienos/farmacologia , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Células PC12 , Temperatura
11.
Foods ; 11(23)2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36496643

RESUMO

BACKGROUND: This study evaluated the influence of intramuscular connective tissue (IMCT) on structural shrinkage and water loss during cooking. Longissimus thoracis (LT), semimembranosus (SM) and semitendinosus (ST) muscles were cut and boiled for 30 min in boiling water, followed by detection of water holding capacity (WHC), tenderness, fiber volume shrinkage and protein denaturation. RESULTS: Compared with LT and SM, ST had the best WHC and lowest WBSF and area shrinkage ratio. The mobility of immobilized water (T22) was key to holding the water of meat. ST contained the highest content of total and heat-soluble collagen. On the contrary, ST showed the lowest content of cross-links and decorin, which indicate the IMCT strength of ST is weaker than the other two. The heat-soluble collagen is positively correlated to T22. CONCLUSIONS: The shrinkage of heat-insoluble IMCT on WHC and WBSF may partly depend on the structural strength changes of IMCT components rather than solely caused by quantitative changes of IMCT.

12.
Sensors (Basel) ; 22(24)2022 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-36560347

RESUMO

Distributed acoustic sensing (DAS) is an emerging technology for recording vibration signals via the optical fibers buried in subsurface conduits. Its relatively easy-to-deploy and high spatial and temporal sampling characteristics make DAS an appealing tool to record seismic wavefields at higher quantity and quality than traditional geophones. Considering that the usage of optical fibers in the urban environment has drawn relatively less attention aside from its functionality as a telecommunication cable, we examine its ability to record seismic signals and investigate its preliminary application in city traffic monitoring. To solve the problems that DAS signals are prone to a variety of environmental noise and are generally of weak amplitude compared to noise, we propose a fast workflow for real-time DAS data processing, which can enhance the detection of regular car signals and suppress the other components. We conduct a DAS experiment in Hangzhou, China, a typical metropolitan area that can provide us with a rich data library to validate our DAS data-processing workflow. The well-processed data enable us to extract their slope and coherency attributes that can provide an estimate of real traffic situations. The one-minute (with video validations) and 24 h statistics of these attributes show that the speed and volume of car flow are well correlated demonstrates the robustness of the proposed data processing workflow and great potential of DAS for city traffic monitoring with high precision and convenience. However, challenges also exist in view that all the attributes are statistically analyzed based on the behaviors of a large number of cars, which is meaningful but lacking in precision. Therefore, we suggest developing more quantitative processing and analyzing methods to provide precise information on individual cars in future works.


Assuntos
Automóveis , Ruído , Cidades , China
13.
PLoS One ; 17(12): e0277169, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36490251

RESUMO

The analysis of urban spatial form is the basic research of urban development. Traditional fractal research often focuses on the urban spatial layout, which cannot visually express the specific form, change characteristics and development trend of urban architectural spaces.The urban architectural form is simplified and the basic architectural form templates are extracted, and then, the correlations between architecture form and fractal dimension are built. The results of the case study show that the architectural layout of Zhengzhou City exhibits obvious fractal characteristics, and the combination of the two-dimensional and three-dimensional fractal dimensions is helpful for comprehensively revealing the architectural layout information. Moreover, the fractal dimension of buildings shows that the gradient from the inner to outer ring decreases, similar to the 'annual growth rings' of trees. Obvious differences exist in the fractal dimensions of urban buildings in different directions, reflecting the urban expansion direction. This study promotes the visualization of fractal theory and the expression of fractal theory in spatial gradient, providing theoretical and data reference for urban spatial form optimization.


Assuntos
Fractais , Reforma Urbana , Cidades , Análise Espacial
14.
Discov Oncol ; 13(1): 138, 2022 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-36512117

RESUMO

Lung adenocarcinoma is the major subtype of lung cancer, accounting for approximately 40% of lung cancers. During clinical treatment, the emergence of chemotherapy resistance seriously affects the effectiveness of treatment. Thus, finding new chemotherapeutic sensitizers is considered to be one of the effective solutions. Biochanin A, as a naturally occurring isoflavone, has been demonstrated to exhibit anticancer effects in various tumors. However, the potential mechanisms of Biochanin A to inhibit tumor development have not been clarified. In the present study, we found that the combinational treatment of cisplatin and Biochanin A exhibited strong synergistic repression on lung adenocarcinoma growth and progression in vitro and in vivo. Considering that epithelial-mesenchymal transition (EMT) is recognized to be associated with both chemoresistance and metastasis, we examined the EMT-related markers and found that Biochanin A could specifically inhibit the expression of ZEB1. Importantly, Biochanin A chemosensitizes lung adenocarcinoma and inhibits cancer cell metastasis by suppressing ZEB1. At the molecular level, Biochanin A affects the stability of ZEB1 protein through the deubiquitination pathway and thereby influences the progression of lung adenocarcinoma. In conclusion, our finding elucidates the potential efficacy of Bichanin A as a chemosensitizer and provides new strategy for the chemotherapy of advanced lung adenocarcinoma.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36520216

RESUMO

PURPOSE: To predict survival prognosis of renal cell carcinoma (RCC) patients with tumors larger than 7 cm by preoperative radiological morphological features. METHODS: We reviewed the medical records of RCC patients with tumors larger than 7 cm from 2007 to 2017 in Zhongshan Hospital, Fudan University. A total of 251 patients' clinical data were collected. 25 and 9 patients were excluded due to loss of follow-up and lack of imaging data, respectively. PFS and OS from date of surgery were evaluated. We defined the irregularity of the tumor as the morphological feature studied and quantified it according to a theorem of the ellipse: the length from the midpoint of the ellipse to any point on the ellipse is shorter than or equal to 1/2 of the long axis. The cutoff value of irregularity was calculated based on the ROC curve. Cox proportional hazards regression models were used to test associations between features and outcome. RESULTS: Of all the 217 patients included in the study, 67 patients had disease progression and 30 patients died. The cutoff value of the irregularity was selected to be 0.5335. Adrenal invasion, presence of distant metastasis and irregularity of tumors were significantly associated with PFS, and presence of distant metastasis and irregularity of tumors were significantly associated with OS. CONCLUSIONS: For patients with tumors larger than 7 cm in RCC, we found a radiological index that is closely related to the prognosis: irregularity. This is an unreported independent prognostic risk factor that can be quantified before surgery.

16.
Toxics ; 10(12)2022 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-36548599

RESUMO

Increasing evidence indicates that endocrine-disrupting chemicals (EDCs) cause a variety of adverse health outcomes and contribute to substantial disease burden. This study summarized the exposure status of polybrominated diphenyl ethers (PBDEs) and phthalates (PAEs) in China and evaluated the disease burden attributable to PBDEs and PAEs in 2015. The results showed that PBDE and PAE concentrations were higher in coastal areas. The disease burden attributable to PBDEs was 0.77 million cases, and the economic costs were CNY 18.92 billion. Meanwhile, 3.02 million individuals suffered from diseases attributable to PAEs, and the economic costs were CNY 49.20 billion. The economic burden caused by PBDEs and PAEs accounted for 0.28% and 0.72% of China's Gross Domestic Product (GDP) in 2015, respectively. When comparing China's results from 2010, it was determined that the GDP ratio of economic costs caused by PAEs in 2015 (0.72%) was lower than in 2010 (1.42%). Finally, compared with the results of the European Union and North America, the GDP ratios of economic costs caused by PAEs in 2015 were 0.19% in Canada (lower than China), 0.29% in the United States (lower than China), and 1.44% in the European Union (higher than China). This study provides important reference values for China's health governance, and further research should be conducted in the future.

17.
Front Surg ; 9: 1005898, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425892

RESUMO

Objective: To compare the return to work (RTW) time between single-port laparoscopic surgery (SPLS) and multiport laparoscopic surgery (MPLS) for benign ovarian tumors. Methods: A cross-sectional cohort study was conducted, which consisted of 335 women of reproductive age with benign ovarian tumors and who were keen on returning to work as early as possible. Surgical outcomes, postoperative pain score, postoperative satisfaction with the cosmesis score (CS), and the RTW time of the SPLS group were compared with those of the MPLS group. Besides, the RTW time and CS were calculated from the questionnaire survey by a single specialized gynecologist. Results: Women who met the inclusion criteria were included in the SPLS (n = 106) and MPLS groups (n = 229). The RTW time in the SPLS group (22.13 ± 27. 06 days) was significantly shorter than that in the MPLS group (46.08 ± 57.86 days) (P < 0.001). The multivariate Cox analysis results showed that age (HR = 0.984, 95% CI, 0.971-0.997, P = 0.020), SPLS (HR = 3.491, 95% CI, 2.422-5. 032, P < 0.001), and return to normal activity time (HR = 0.980, 95% CI, 0.961-0.998, P = 0.029) were independent factors of the RTW time. Conclusions: SPLS may be advantageous in terms of shortening the RTW time for women with benign ovarian tumors.

18.
Cell Death Dis ; 13(11): 978, 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36402747

RESUMO

Neoadjuvant radiotherapy is a standard treatment for locally advanced rectal cancer, however, resistance to chemoradiotherapy is one of the main obstacles to improving treatment outcomes. The goal of this study was to explore the role of PRDM15 involved in the radioresistance of colorectal cancer and to clarify the underlying mechanism. In present study, we demonstrated that, after DNA damage, PRDM15 was upregulated and localized to DNA damage sites, co-localizing with γ-H2AX. Knockdown of PRDM15 inhibited DNA damage repair and increased radiosensitivity in colorectal cancer cells. Mechanistically, PRDM15 promoted DNA repair by interacting with DNA-PKcs and Ku70/Ku80 complex. In preclinical models of rectal cancer, knockdown of PRDM15 sensitized cell derived xenograft and patient derived xenograft to radiotherapy. In 80 rectal cancer patients treated with neoadjuvant chemoradiotherapy, higher PRDM15 expression was observed associated with weaker tumor regression and poorer prognosis. Our findings revealed that inhibiting PRDM15 was potent to overcome radioresistance through abrogating DNA repair in colorectal cancer cells. Additionally, the expression level of PRDM15 could be applied to predict radiotherapy responsiveness and the outcome of neoadjuvant radiotherapy in rectal cancer patients.


Assuntos
Reparo do DNA , Neoplasias Retais , Humanos , Neoplasias Retais/genética , Neoplasias Retais/radioterapia , Dano ao DNA , Tolerância a Radiação , DNA , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Fatores de Transcrição
19.
J Am Chem Soc ; 144(48): 22067-22074, 2022 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-36416740

RESUMO

Terpene cyclases (TCs), extraordinary enzymes that create the structural diversity seen in terpene natural products, are traditionally divided into two classes, class I and class II. Although the structural and mechanistic features of class I TCs are well-known, the corresponding details in class II counterparts have not been fully characterized. Here, we report the genome mining discovery and structural characterization of two class II sesquiterpene cyclases (STCs) from Streptomyces. These drimenyl diphosphate synthases (DMSs) are the first STCs shown to possess ß,γ-didomain architecture. High-resolution X-ray crystal structures of DMS from Streptomyces showdoensis (SsDMS) in complex with both a farnesyl diphosphate and Mg2+ unveiled an induced-fit mechanism, with an unprecedented Mg2+ binding mode, finally solving one of the lingering questions in class II TC enzymology. This study supports continued genome mining for novel bacterial TCs and provides new mechanistic insights into canonical class II TCs that will lead to advances in TC engineering and synthetic biology.

20.
Environ Sci Technol ; 56(23): 17018-17028, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36375127

RESUMO

The toxicity of organophosphate esters (OPEs) on embryonic development is well noted in animal experiments, but epidemiological studies are still lacking. This study evaluated the prenatal exposure of OPEs and its trimester-specific and gender-specific effects on fetal growth. The correlations between OPE exposure and fetal growth were investigated by linear mixed-effect models and multivariable linear regression analyses. Prenatal exposure to tributyl phosphate (TBP) was negatively associated with a z-score of fetal abdominal circumference (AC), biparietal diameter (BPD), femur length (FL), and head circumference (HC). In the second trimester, the serum concentration of TBP was inversely related to the z-score of AC, BPD, and HC. In the third trimester, serum concentration of TBP was inversely related to AC, BPD, and FL z-scores. Prenatal exposure to tri-m-cresyl phosphate (TMCP) was inversely related to the z-score of AC, BPD, and HC. In the second trimester, TMCP was negatively correlated with AC, BPD, FL, and HC z-scores. After stratification by gender, male fetuses were more sensitive to OPE exposure. The above results remained robust after excluding pregnant women who gave preterm birth or those with low or high pre-pregnancy BMI. Our findings suggested that health effects of typical OPEs, particularly TBP and TMCP, should be taken into consideration in future works.


Assuntos
Nascimento Prematuro , Efeitos Tardios da Exposição Pré-Natal , Recém-Nascido , Gravidez , Masculino , Feminino , Humanos , Ultrassonografia Pré-Natal , Desenvolvimento Fetal , Organofosfatos/toxicidade , Ésteres
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