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1.
Arch Gynecol Obstet ; 2020 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-32417959

RESUMO

PURPOSE: The present study aimed to investigate the effects of miR-504 in cervical cancer. METHODS: Normal and cervical cancer tissue specimens derived from TCGA and GTEx databases were employed to analyze the miR-504 and PAICS (one of potential target gene of miR-504) expression. Kaplan-Meier strategy was applied to analyze the prognostic powers of miR-504 and PAICS. The proliferation, clonogenic ability, invasion, and migration of cervical cancer cells (C-33A and HeLa) were detected using Cell Counting Kit 8, colony formation, and transwell assays. Pearson correlation analysis was used to assess the correlation between miR-504 and PAICS, which was confirmed using luciferase reporter assay. The mRNA and protein levels were detected by qRT-PCR and western blot, respectively. RESULTS: TCGA data revealed that miR-504 expression might be decreased in cervical cancer, which was correlated with unfavorable prognosis. Further experiments exhibited that abnormal miR-504 expression negatively affected malignant cellular behaviors in cervical cancer, including proliferation, colony formation, invasion, and migration. PAICS was identified as a putative target of miR-504, and negatively related with miR-504 expression. PAICS expression was increased in cervical cancer and its high-regulation-induced worse outcomes of patients with cervical cancer. Rescue experiments indicated that PAICS restricted the impacts of miR-504 in cervical cancer cells. Analysis of western blot suggested that overexpression of PAICS overturned the miR-504-induced EMT inactivation. CONCLUSION: Our observations elucidated that miR-504, acting as a suppressor for the progression of cervical cancer, inhibits cell proliferation, invasion and migration, and mediates EMT via negatively regulating PAICS.

2.
J Hazard Mater ; 396: 122731, 2020 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-32339877

RESUMO

With the development of nuclear power, the negative environmental impact such as radioactive pollution has become an urgent issue to impede the utilization of nuclear energy. The construction of promising organic-inorganic hybrid materials is considered as an effective strategy for environmental remediation of radioactive contamination. In this work, two-dimensional transition metal carbide (MXene), an emerging inorganic layered material, has been successfully modified by carboxyl terminated aryl diazonium salt to both enhance its chelating ability to radionuclides and improve its water stability. The carboxyl functionalized Ti3C2Tx MXene (TCCH) shows excellent removal ability for U(VI) and Eu(III), evidenced by ultrafast adsorption kinetics (3 min), high maximum adsorption capacities (344.8 mg/g for U and 97.1 mg/g for Eu) and high removal percentage of radionuclides from artificial groundwater (> 90%). The adsorption of U(VI) and Eu(III) on TCCH are in good accord with the Langmuir adsorption isotherm model and the pseudo-second-order kinetic model. Ionic strength experiments, X-ray photoelectron spectroscopy (XPS) and Extended X-ray absorption fine structure (EXAFS) analyses were conducted to assess the detailed adsorption mechanism. The results reveal that the adsorption of U(VI) on TCCH follows an inner-sphere configuration, whereas the adsorption of Eu(III) is determined by both inner-sphere complexation and electrostatic interaction.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32150379

RESUMO

Despite that two-dimensional transition metal carbides and carbonitrides (MXenes) are burgeoning candidates for remediation of environmental pollutants, the construction of robust functionalized MXene nanosheets with a high affinity for target heavy metal ions and radionuclides remains a challenge. Here we report the successful placement of amidoxime chelating groups on Ti3C2Tx MXene surface by diazonium salt grafting. The introduction of amidoxime functional groups significantly enhances the selectivity of Ti3C2Tx nanosheets for uranyl ions and also greatly improves their stability in aqueous solution, enabling efficient, rapid, and recyclable uranium extraction from aqueous solutions containing competitive metal ions. Benefiting from the excellent conductivity of MXenes, the amidoxime functionalized Ti3C2Tx nanosheets show outstanding electrochemical performance such that when loaded on carbon cloth the application of an electric field increases the uranium adsorption capacity from 294 to 626 mg/g, outperforming all organic electrochemical sorption materials reported previously. The present work provides an effective strategy to functionalize MXene nanosheets with fundamental implications for the design of MXene-based selective electrosorption electrode materials.

4.
J Chromatogr Sci ; 58(5): 471-476, 2020 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-32016423

RESUMO

This study established a rapid and reliable method to determine chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) residues in Chinese gelatin medicines. CAP, TAP and FF were extracted from medicine samples using 2% (v/v) ammonium hydroxide in acetonitrile. Trypsin was used to eliminate the matrix effect caused by protein components in gelatin medicines, whereas anhydrous sodium sulfate, C18-N and NH2-PSA adsorbents were applied to reduce matrix effect induced by other components. The analytical method of these drugs was optimized on ultra high-performance liquid chromatography-mass spectrometer (UHPLC-MS/MS) through the analysis of their standard linearity and regression. The optimized extraction and analytical method were validated in one Chinese gelatin medicine sample (Colla corii asini, E Jiao) with three fortification levels (2, 5 and 10 µg/kg), and the recoveries of these drug residues ranged of 87.6-102.7%. The limit of detection and quantification of CAP, TAP and FF in the sample were 0.2 and 0.5 µg/kg, 0.4 and 1.5 µg/kg, and 0.5 and 1.5 µg/kg, respectively. A total of 30 Chinese gelatin medicine samples were analyzed using the established method. No drug residues were found in these samples except for one Testudinis Carapacis et Plastri (1.67 µg/kg FF) and one turtle shell glue (2.55 µg/kg FF).

5.
Acta Pharmacol Sin ; 41(4): 516-522, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32047262

RESUMO

Excessive nitric oxide (NO) causes extensive damage to the nervous system, and the adrenergic system is disordered in many neuropsychiatric diseases. However, the role of the adrenergic system in protection of the nervous system against sodium nitroprusside (SNP) injury remains unclear. In this study, we investigated the effect of ganoderic acid A (GA A) against SNP injury in neural cells and the role of adrenergic receptors in GA A neuroprotection. We found that SNP (0.125-2 mM) dose-dependently decreased the viability of both SH-SY5Y and PC12 cells and markedly increased NO contents. Pretreatment with GA A (10 µM) significantly attenuated SNP-induced cytotoxicity and NO increase in SH-SY5Y cells, but not in PC12 cells. Furthermore, pretreatment with GA A caused significantly higher adrenaline content in SH-SY5Y cells than in PC12 cells. In order to elucidate the mechanism of GA A-protecting SH-SY5Y cells, we added adrenaline, phentolamine, metoprolol, or ICI 118551 1 h before GA A was added to the culture medium. We found that addition of adrenaline (10 µM) significantly improved GA A protection in PC12 cells. The addition of ß1-adrenergic receptor antagonist metoprolol (10 µM) or ß2-adrenergic receptor antagonist ICI 118551 (0.1 µM) blocked the protective effect of GA A, whereas the addition of α-adrenergic receptor antagonist phentolamine (0.1 µM) did not affect GA A protection in SH-SY5Y cells. These results suggest that ß-adrenergic receptors play an important role in the protection of GA A in SH-SY5Y cells against SNP injuries, and excessive adrenaline system activation caused great damage to the nervous system.

6.
J Phys Chem Lett ; 11(1): 243-248, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31724400

RESUMO

The fundamental understanding of electrochemistry urges accurate knowledge of all interfacial properties at the molecular level, but the retrieval of such information is a real challenge. Optical spectroscopies facilitated by surface plasmon enhancement can shed light on this field, yet past studies relied on either highly inhomogeneous "hot spots" or planar plasmon modes with limited enhancement. Here we report an in situ sum-frequency vibrational spectroscopy scheme using plasmonic nanogratings, which enable strong, coherent surface plasmon excitation even on planar electrodes. With two classical reactions, the gold oxidation and pyridine adsorption in water, we demonstrate the realization of coherent vibrational spectroscopy in the strong absorption region, revealing the polar orientation and ordering of interfacial species that are crucial toward the mechanistic understanding of electrochemical phenomena.

7.
Fitoterapia ; 140: 104417, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31707125

RESUMO

Schefflera rubriflora, a plant native to Yunnan Province in China, is often used to treat ailments such as neuropathic pain, tracheitis, and cough. However, the active components imparting these pharmacological effects are largely unexplored. In this study, five novel lignans and three new derivatives of benzoid or pyran were isolated from the leaves and twigs of S. rubriflora. The structures of these compounds were determined by the comprehensive analyses of the 1D and 2D NMR spectra and ESI mass spectra and a comparison of the obtained data with those of the literature data. All the compounds were tested for the inhibition of IL-6 expression. Three of the isolated compounds could inhibit the expression by 52% to 72%.


Assuntos
Araliaceae/química , Interleucina-6/antagonistas & inibidores , Lignanas/farmacologia , Animais , China , Camundongos , Estrutura Molecular , Compostos Fitoquímicos/farmacologia , Folhas de Planta/química , Células RAW 264.7
8.
Phytochemistry ; 167: 112096, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31470169

RESUMO

Although Morinda umbellata L. has been used in numerous folk medicines, there is a lack of phytochemical studies on this plant. Sixteen undescribed quinones, namely, ten anthraquinones (umbellatas A-J), one naphthohydroquinone (umbellata K), one naphthohydroquinone dimer (umbellata L), and four dinaphthofuran quinones (umbellatas M-P), were isolated from the aerial parts of Morinda umbellata L. (Rubiaceae). The structures of all the isolated quinones were elucidated based on spectroscopic methods. Four of the unknown quinones (umbellatas A, H, K and M) showed potent cytotoxic effects against A431, A2780, NCI-H460, HCT116, HepG2, and MCF-7 human cancer cell lines with IC50 values of 1.3-7.1 µM. These results reveal potential lead compounds for the development of new anticancer agents.


Assuntos
Antineoplásicos/farmacologia , Morinda/química , Componentes Aéreos da Planta/química , Quinonas/farmacologia , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Humanos , Quinonas/química
9.
J Phys Chem Lett ; 10(19): 5943-5948, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31448602

RESUMO

The rich chemistry of oxide/aqueous interfaces originates from the interfacial layer formed by surface charges and adjoining water molecules. Yet not much is clear about such layers, because they are difficult to access, and measurements unavoidably collect signals from the diffuse layer nearby, which is perturbed by the surface potential extending into the bulk water. Here we show that gating of a semiconductor/oxide/water junction can effectively vary the surface charge density at the oxide/water interface but keep the surface potential low and barely varying, allowing effective removal of the diffuse layer contribution. With sum-frequency vibrational spectroscopy on a silicon/silica/deionized-water model junction, the variation of the bonded layer water structure in response to surface charging can be readily detected. This new scheme is generally applicable to all oxide/water interfaces, providing opportunities for future investigations at a deeper molecular level.

10.
Biomed Res Int ; 2019: 2807123, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31080815

RESUMO

Cervical cancer is a life-threatening complication, appearing as the uncontrolled growth of abnormal cells in the lining of the cervix. Every year, increasing numbers of cervical cancer cases are reported worldwide. Different identification strategies were proposed to detect cervical cancer at the earlier stages using various biomarkers. Squamous cell carcinoma antigen (SCC-Ag) is one of the potential biomarkers for this diagnosis. Nanomaterial-based detection systems were shown to be efficient with different clinical biomarkers. In this study, we have demonstrated strontium oxide-modified interdigitated electrode (IDE) fabrication by the sol-gel method and characterized by scanning electron microscopy and high-power microscopy. Analysis of the bare devices indicated the reproducibility with the fabrication, and further pH scouting on the device revealed that the reliability of the working pH ranges from 3 to 9. The sensing surface was tested to detect SCC-Ag against its specific antibody; the detection limit was found to be 10 pM, and the sensitivity was in the range between 1 and 10 pM as calculated by 3σ. The specificity experiment was carried out using major proteins from human serum, such as albumin and globulin. SCC-Ag was shown to be selectively detected on the strontium oxide-modified IDE surface.


Assuntos
Antígenos de Neoplasias/administração & dosagem , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/diagnóstico , Serpinas/administração & dosagem , Estrôncio/administração & dosagem , Neoplasias do Colo do Útero/diagnóstico , Antígenos de Neoplasias/metabolismo , Carcinoma de Células Escamosas/metabolismo , Colo do Útero/metabolismo , Eletrodos , Feminino , Humanos , Recidiva Local de Neoplasia/diagnóstico , Recidiva Local de Neoplasia/metabolismo , Óxidos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Serpinas/metabolismo , Neoplasias do Colo do Útero/metabolismo
11.
Phytochemistry ; 163: 23-32, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30986687

RESUMO

The 95% ethanol extract and its EtOAc and n-BuOH fractions obtained from the leaves and twigs of Schefflera rubriflora C. J. Tseng & G. Hoo showed significant inhibitory activities (33.6%, 35.7% and 40.6%, respectively) against croton oil-induced ear inflammation in mice. Bioactivity-guided isolation and separation gave eight previously undescribed terpenes or terpene glycosides. Structural elucidation was based on UV, IR, and NMR spectroscopy, MS, experimental and calculated ECD data, and Mosher's method. To identify anti-inflammatory components from the extract, all the compounds were evaluated for tumor necrosis factor-α (TNF-α) and interleukine-6 (IL-6) inhibitory activities. Four undescribed compounds inhibited mRNA expression of TNF-α and IL-6 with IC50 values of 15.3-52.4 µM.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Araliaceae/química , Interleucina-6/antagonistas & inibidores , Extratos Vegetais/farmacologia , Terpenos/farmacologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Células Cultivadas , Óleo de Cróton , Relação Dose-Resposta a Droga , Orelha , Edema/induzido quimicamente , Edema/tratamento farmacológico , Interleucina-6/genética , Interleucina-6/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos , Conformação Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Células RAW 264.7 , Relação Estrutura-Atividade , Terpenos/química , Terpenos/isolamento & purificação , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
12.
Environ Sci Technol ; 53(7): 3739-3747, 2019 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-30843686

RESUMO

Environmental contamination by 99Tc(VII) from radioactive wastewater streams is of particular concern due to the long half-life of 99Tc and high mobility of pertechnetate. Herein, we report a novel MXene-polyelectrolyte nanocomposite with three-dimensional networks for enhanced removal of perrhenate, which is pertechnetate simulant. The introduction of poly(diallyldimethylammonium chloride) (PDDA) regulates the surface charge and improves the stability of Ti2CT x nanosheet, resulting in Re(VII) removal capacity of up to 363 mg g-1, and fast sorption kinetics. The Ti2CT x/PDDA nanocomposite furthermore exhibits good selectivity for ReO4- when competing anions (such as Cl- and SO42-) coexist at a concentration of 1800 times. The immobilization mechanism was confirmed as a sorption-reduction process by batch sorption experiments and X-ray photoelectron spectroscopy. The pH-dependent reducing activity of Ti2CT x/PDDA nanocomposite toward Re(VII) was clarified by X-ray absorption spectroscopy. As the pH increases, the local environment gradually changes from octahedral-coordinated Re(IV) to tetrahedral-coordinated Re(VII). The overall results suggest that Ti2CT x/PDDA nanocomposite may be a promising candidate for efficient elimination of Tc contamination. The reported surface modification strategy might result in applications of MXene-based materials in environmental remediation of other oxidized anion pollutants.


Assuntos
Nanocompostos , Ânions , Titânio , Tomografia Computadorizada por Raios X , Espectroscopia por Absorção de Raios X
13.
Appl Environ Microbiol ; 85(9)2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-30824451

RESUMO

Crown gall disease caused by Agrobacterium tumefaciens severely impacts the production of peach and other fruit trees. Several peach cultivars are partially resistant to A. tumefaciens, but little is known about the roles of endophytic microbiota in disease resistance. In the present study, the endophytic bacterial communities of resistant and susceptible peach cultivars "Honggengansutao" and "Okinawa" were analyzed using universal 16S rRNA gene amplicon sequencing in parallel with the cultivation and characterization of bacterial isolates. A total of 1,357,088 high-quality sequences representing 3,160 distinct operational taxonomic units (OTUs; Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes) and 1,200 isolates of 20 genera and 305 distinct ribotypes were collected from peach roots and twigs. It was found that factors including plant developmental stage, cultivar, and A. tumefaciens invasion strongly influenced the peach endophytic communities. The community diversity of endophytic bacteria and the abundance of culturable bacteria were both higher in the roots of the resistant cultivar, particularly after inoculation. Strikingly, the pathogen antagonists Streptomyces and Pseudomonas in roots and Rhizobium in twigs were most frequently detected in resistant plants. Our results suggest that the higher abundance and diversity of endophytic bacteria and increased proportions of antagonistic bacteria might contribute to the natural defense of the resistant cultivar against A. tumefaciens This work reveals the relationships between endophytic bacteria and disease resistance in peach plants and provides important information for microbiome-based biocontrol of crown gall disease in fruit trees.IMPORTANCE Agrobacterium tumefaciens as the causal agent of peach crown gall disease can be controlled by planting resistant cultivars. This study profiles the endophytic bacteria in susceptible and resistant peach cultivars, advancing our understanding of the relationships between endophytic bacterial communities and peach crown gall disease, with potential implications for other complex microbiome-plant-pathogen interactions. The resistant cultivar may defend itself by increasing the diversity and abundance of beneficial endophytic bacteria. The antagonists identified among the genera Streptomyces, Pseudomonas, and Rhizobium may have application potential for biocontrol of crown gall disease in fruit trees.

14.
Se Pu ; 37(2): 177-182, 2019 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-30693725

RESUMO

An ultra-performance liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction (dSPE-UPLC-MS/MS) method was developed to determine seven non-selective cyclooxygenase inhibitors in milk powder. The samples were extracted with 0.01 mol/L pH 2.5 ascorbic acid-acetonitrile-ethyl acetate solution (2:5:5, v/v/v), and then purified with a mixture of anhydrous sodium sulfate, octadecyl carbon silica gel (C18-N) sorbent and ethylenediamine-N-propyl carbon silica gel (NH2-PSA) sorbent. The analysis was performed using a UPLC-MS/MS system with Waters CORTECS UPLC C18 (100 mm×2.1 mm, 1.6 µm) column. The mobile phase consisted of 0.1% (v/v) formic acid aqueous solution and acetonitrile containing 0.1% (v/v) formic acid by gradient elution, and the multiple reaction monitoring (MRM) mode was used. Five linear calibration curves were obtained with correlation coefficients (r2) ≥ 0.9965. The recoveries were determined at three spiked levels and ranged from 76.4% to 89.8%. The limits of quantification (LOQs) were from 2 to 5 µg/kg. This method is suitable for the determination of seven non-selective cyclooxygenase inhibitors in milk powder.


Assuntos
Inibidores de Ciclo-Oxigenase/análise , Contaminação de Alimentos/análise , Leite/química , Animais , Cromatografia Líquida de Alta Pressão , Pós , Extração em Fase Sólida , Espectrometria de Massas em Tandem
15.
Bioorg Chem ; 87: 867-875, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30528930

RESUMO

Eight new glycosides, morindaparvins P-W, were isolated from the butanol extract of the aerial parts of Morinda parvifolia. These new structures were elucidated by comprehensive spectroscopic analysis and by comparing the experimental and calculated electronic circular dichroism spectra. The butanol extract was observed to significantly reduce the levels of alanine aminotransferase, aspartate transaminase, and lactate dehydrogenase in the sera of mice with concanavalin A-induced hepatitis. At a concentration of 10 µM, five compounds (1, and 4-7) isolated from the butanol extract possessed hepatoprotective activities against the damage induced by acetaminophen in human HepG2 liver cancer cells.

16.
Plant Dis ; 102(9): 1841-1847, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30125152

RESUMO

China accounts for over 50% of apple production worldwide. Very recently, a novel ilarvirus, Apple necrotic mosaic virus (ApNMV), was isolated from apple trees showing mosaic symptoms in Japan. This study compared different types of mosaic symptoms observed in apple trees in China under field conditions. Complete nucleotide sequences were obtained for six isolates of ApNMV. The genomic components varied in size from 3,378 to 3,380 nt (RNA1), 2,778 to 2,786 nt (RNA2), and 1,909 to 1,955 nt (RNA3), respectively. Although nucleotide sequence similarities with subgroup 3 ilarviruses were low (49.2 to 64.3%), results of phylogenetic analysis indicated that Chinese ApNMV isolates were clustered in subgroup 3 together with Prunus necrotic ring spot virus (PNRSV) and Apple mosaic virus (ApMV). Apple mosaic disease occurred widely in apple producing areas of China with a very high percentage (92.1%, 268 out of 291) of symptomatic trees being infected with ApNMV but not with ApMV. The data suggested that ApNMV might be the main pathogen causing apple mosaic disease in China. The genomes of the six studied Chinese ApNMV isolates demonstrated substantial sequence diversity. Here, we demonstrated a strong association of ApNMV with the mosaic disease of apple trees in China.


Assuntos
Variação Genética , Genoma Viral/genética , Ilarvirus/genética , Malus/virologia , Doenças das Plantas/virologia , Ilarvirus/isolamento & purificação , Filogenia , Análise de Sequência
17.
Phytochemistry ; 152: 97-104, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29758523

RESUMO

Eight previously undescribed naphthohydroquinone glycosides, namely morindaparvins H-O, together with four known anthraquinone glycosides were isolated from the n-BuOH extract of the aerial parts of Morinda parvifolia Bartl. ex DC (Rubiaceae). The structures of morindaparvins H-O were elucidated on the basis of spectroscopic analysis. To our knowledge, this is the first isolation of quinone glycosides from the plant M. parvifolia. The results showed that all 12 compounds at the concentration of 50 µM significantly increased p53 mRNA expression in A2780 cells compared with the blank control group.


Assuntos
Antraquinonas/farmacologia , Glicosídeos/farmacologia , Hidroquinonas/farmacologia , Componentes Aéreos da Planta/química , RNA Mensageiro/genética , Proteína Supressora de Tumor p53/genética , Antraquinonas/química , Antraquinonas/isolamento & purificação , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Hidroquinonas/química , Hidroquinonas/isolamento & purificação , Estrutura Molecular , Morinda/química
18.
Zhongguo Zhong Yao Za Zhi ; 43(1): 114-118, 2018 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29552820

RESUMO

Seventeen compounds were isolated from n-butanol extract of the leaves of Moringa oleifera, using column chromatography over macroporous resin HP-20,Sephadex LH-20, and ODS. Their structures were identified as two carboline,tangutorid E(1) and tangutorid F(2); three phenolic glycosides,niazirin(3),benzaldehyde 4-O-α-L-rhamnopyranoside(4) and 4-O-ß-D-glucopyranosidebenzoic acid(5); four chlorogenic acid and derivatives,4-caffeoylquinic acid(6),methyl 4-caffeoylquinate(7),caffeoylquinic acid(8) and methyl caffeoylquinate(9); two nucleosids,uridine(10) and adenosine(11); one flavone,quercetin 3-O-ß-D-glucopyranoside(12); five other types of compounds,phthalimidineacetic acid(13),3-pyridinecarboxamide(14),3,4-dihydroxy-benzoic acid(15),5-hydroxymethyl-2-furancarboxylic acid(16) and 5-hydroxymethyl-2-furaldehyde(17) by the spectral data of ¹H, ¹³C-NMR and MS. Among them,compounds 1-2,7,9-10,16 and 17 were isolated from M. oleifera for the first time.


Assuntos
Glicosídeos/análise , Moringa oleifera/química , Fenóis/análise , Extratos Vegetais/química , Folhas de Planta/química , 1-Butanol , Compostos Fitoquímicos/análise
19.
J Virol Methods ; 254: 13-17, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29407207

RESUMO

Three of the five well-known viroids infecting grapevine belong to the genus Apscaviroid. Grapevine latent viroid (GLVd) is a novel grapevine viroid. Although GLVd has the typical sequence motifs of the genus Apscaviroid, it is still an unassigned viroid. In this study, a sensitive, convenient, and rapid one-step RT-qPCR method using hydrolysis probes for the detection of GLVd was developed. Survey and bioassays were also performed for this viroid. Using this method in the survey of GLVd, a low infection rate of 2/226 in a grapevine germplasm resource nursery and a demonstration vineyard in China was determined. Bioassays using agroinfiltration showed that GLVd can infect 'Kyoho' grapevine but not any of the tested herbaceous plants. Furthermore, sequence variability of GLVd was analyzed in six GLVd-infected grapevines. Sequencing revealed a predominant variant with only a few nucleotide changes compared with the reference variant of GLVd. Therefore, the developed RT-qPCR method should be helpful for determining GLVd in other vineyards of the world. The low infection rate, host range, and sequence variability of GLVd have important implications to further improve our knowledge on this novel grapevine viroid.


Assuntos
Reação em Cadeia da Polimerase em Tempo Real , Viroides/genética , Doenças das Plantas/virologia , RNA Viral , Reação em Cadeia da Polimerase em Tempo Real/métodos , Vitis/virologia
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 190: 104-110, 2018 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-28917162

RESUMO

A reversible Schiff's base fluorescence probe for Al3+, (3,5-dichloro-2- hydroxybenzylidene) quinoline-2-carbohydrazide (QC), based on quinoline derivative has been designed, synthesized and evaluated. The QC exhibited a high sensitivity and selectivity toward Al3+ in EtOH-H2O (v/v=1:9, pH=6) by forming a 1:1 complex with Al3+ and the detection limit of QC for Al3+ was as low as 0.012µM. Furthermore, these results displayed that the binding of QCAl3+ was broken by F-, so this system could be used to monitor F- in the future. The enhancement fluorescence of the QC could be attributed to the inhibition of PET and ESIPT and the emergency of CHEF process induced by Al3+. More importantly, QC was not only successfully used for the determination of trace Al3+ in the tap water and the human blood serum, but was valid for fluorescence imaging of Al3+ in the Hela cells.


Assuntos
Alumínio/análise , Corantes Fluorescentes/química , Imageamento Tridimensional , Quinolinas/química , Bases de Schiff/química , Alumínio/sangue , Ânions , Morte Celular , Células HeLa , Humanos , Quinolinas/síntese química , Bases de Schiff/síntese química , Espectrometria de Fluorescência , Água/química
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