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1.
Rev Environ Contam Toxicol ; 252: 1-50, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31451946

RESUMO

Microbe-assisted organopollutant removal, or in planta crop decontamination, is based on an interactive system between organopollutant-degrading endophytic bacteria (DEBOP) and crops in alleviating organic toxins in plants. This script focuses on the fast-growing body of literature that has recently bloomed in organopollutant control in agricultural plants. The various facets of DEBOP under study include their colonization, distribution, plant growth-promoting mechanisms, and modes of action in the detoxification process in plants. Also, an assessment of the biotechnological advances, advantages, and bottlenecks in accelerating the implementation of this decontamination strategy will be undertaken. The highlighted key research directions from this review will shape the future of agro-environmental sustainability and preservation of human health.


Assuntos
Bactérias , Produtos Agrícolas/microbiologia , Endófitos , Poluentes do Solo/metabolismo , Agricultura , Produtos Agrícolas/metabolismo , Inativação Metabólica , Desenvolvimento Vegetal
2.
J Hazard Mater ; 382: 121056, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31470305

RESUMO

We report a new 7-nitrobenzo-2-oxa-1, 3-diazolyl (NBD)-based chemosensor containing a piperazine derivative, NBDP, for detection of mercury ions in almost 100% aqueous medium. The chemosensor shows sensing exclusively toward Hg2+ with a switch-on fluorescence response at 543 nm, which could be attributed to the blocking of PET (photo-induced electron transfer) process upon complexation with mercury ions. The molar ratio of Hg(Ⅱ) to NBDP in the complex is 1:1 based on the Job's plot and HRMS studies. Optimized configurations of NBDP and NBDP-Hg2+ complexes were simulated by means of DFT calculations. The reversible fluorescence response with low detection limit (19.2 nM) in the pH range of 6.0-7.5 renders NBDP a promising candidate for Hg2+ detection in neutral aqueous environments. For the practical application of the chemosensor, test strips were successfully fabricated for rapid detection of Hg2+ ions. Moreover, the utility of NBDP showing the mercury recognition in Human liver cancer cells (SMMC-7721) and zebrafish as well as in live tissues of Arabidopsis thaliana has been demonstrated as monitored by fluorescence imaging.

3.
J Nanosci Nanotechnol ; 20(3): 1613-1621, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492323

RESUMO

Nano-composite films were developed between silver nanoparticles (Ag NPs) and a low-density polyethylene (LDPE) using master batches by melt extruding and melt compounding. The Ag/PE composite film showed decreased gas permeability, moisture permeability coefficient, the tear strength, the longitudinal and transverse elongation to that of commercial LDPE. Although stiffness increased at high Ag (40 ppm) concentration, but the longitudinal and transverse tensile strength enhance comparing with commercial PE. Light transmittance and haze were comparable. Both Nano-silver and composite films are effective against Escherichia coli (E. coli). Antibacterial activity of nano-silver for E. coli was determined by diameter of the inhibition zone and the minimum inhibitory concentration of nano-silver is detected by tube double dilution method reaching 15.63 ppm. The composite films are effective inhibition of E. coli at concentrations of 40 ppm Ag nanoparticles. Moreover, Nano-silver migration occurs in composite film. One-side migration was conducted to detect under three food simulants (3% acetic acid, 50% ethanol and distilled water) at three degree of temperature (25 °C, 40 °C and 70 °C) on different period of time (2, 4, 6, 8, 10 and 12 hours). These results indicated that the highest migration amount was obtained with 3% acetic acid following distilled water and finally 50% ethanol under same conditions. The migration level is dependent upon time and temperature and high migration time and temperature can enhance migration level. These findings demonstrate that nano-silver-containing polyethylene composite film may have a great potential for developing antibacterial and acid food packaging system.

4.
Water Res ; 168: 115158, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31618695

RESUMO

Elevated concentrations of polycyclic aromatic hydrocarbons (PAHs) have been observed with rapid agricultural and industrial development in the Songnen Plain, Northeast China, but the prospective sources have not been yet apportioned. The concentration of PAHs was measured in 31 sediment samples from 11 Songnen Plain lakes in 2015. The background flux of PAHs in these lake sediments is < 463 µg m-2 year-1. The maximal concentration of 16 U.S. EPA priority PAHs (599 ng g-1) recorded in this study is lower or similar to that found in most of the lake sediments across China, but higher than remote areas, such as North America Rocky Mountains. Both concentration and flux of PAHs increased after the 1950s, which correspond to the industrial development in this area and would probably mark the beginning of the Anthropocene in this region. A chemical mass balance model estimated that straw burning was a major source of Σ13PAH (3-6 rings) during the past 200 years, with an average contribution of 22.1%, followed by forest fire (21.2%), burning of gasoline (19.1%), coal (12.2%), coke (4.8%) and diesel (3.9%), whereas the contribution from crude oil and natural gas was negligible (<1%). Straw burning (20.2-25.2%) and forest fire (16.7-30.6%) were major sources of PAHs and contributed increasing flux in the past 200 years. The elevated level of PAH recorded after 1950s in this region are also from burning of gasoline (26.1-26.4%), coal (15.3-15.8%), and coke (5.1-9.0%). The contribution of petrogenic sources (e.g., direct oil spill) to the concentration of Σ13PAH seemed to be ignorable, at least in these lakes.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , China , Monitoramento Ambiental , Sedimentos Geológicos , Lagos , Estudos Prospectivos
5.
Lancet Oncol ; 20(11): e619-e626, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31674320

RESUMO

As a result of recent, substantial capacity building, a new landscape for cancer drug trials is emerging in China. However, data on the characteristics of cancer drug trials, and how they have changed over time, are scarce. Based on clinical trials published on the China Food and Drug Administration Registration and Information Disclosure Platform for Drug Clinical Studies, we aimed to systematically review changes over time in clinical trials of cancer drugs in mainland China from 2009 to 2018, to provide insight on the effectiveness of the pharmaceutical industry and identify unmet clinical needs of stakeholders. A total of 1493 trials of 751 newly tested cancer drugs were initiated. Increases over time were observed for the annual number of initiated trials, newly tested drugs, and newly added leading clinical trial units, with a sharp increase after 2016. Of the 1385 trials in which cancer types were identified, solid tumours (325 [23%] trials), non-small-cell lung cancer (232 [17%]), and lymphoma (126 [9%]) were the most common. A markedly uneven distribution was also observed in the geography of leading units with the largest number of leading units located in east China (50 [41%]) and the smallest number located in southwest China (4 [3%]). The growth trends we observed illustrate the progress in and increasing capability of cancer drug research and development achieved in mainland China over the decade from 2009. The low number of clinical trials on tumours with epidemiological characteristics unique to the Chinese population and the unbalanced geographical distribution of leading clinical trial units will provide potential targets for policy makers and other stakeholders. Further research efforts should address cancers uniquely relevant to Chinese populations, globally rare cancers, and the balance between equitable drug access, efficiency, and sustainability of cancer drug research and development in mainland China.

6.
Appl Opt ; 58(28): 7781-7788, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674460

RESUMO

The temperature stability of the optical fiber voltage transformer (OVT) has always been the key factor restricting its practical application. There are many factors affecting temperature characteristics, including residual birefringence during Bi4Ge3O12 (BGO) processing, the thermo-optic effect, the thermal expansion effect, and temperature change of the second-order nonlinear polarization coefficient. These complex effects interact with each other and affect the temperature characteristics of the OVT. Therefore, for a long time, the research of various literatures has not been comprehensive enough, and even has certain contradictions. In this paper, the mechanism between the five influencing factors and the OVT temperature characteristics was investigated. The experimental results show that the temperature characteristics of OVT can be divided into two aspects: the starting point and the conversion coefficient. The starting point is affected by the residual birefringence of the BGO crystal, crystal alignment, the thermal expansion of the crystal, and the thermo-optic effect. The conversion coefficient is affected by the starting point, the thermo-optic effect and the second-order nonlinear polarization coefficient.

7.
Opt Lett ; 44(21): 5338-5341, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31675002

RESUMO

We present a simulation method to reproduce the damage crater formation and particle ejection phenomena observed in the laser-induced surface damage process of potassium dihydrogen phosphate (KDP) crystals. Based on the smoothed particle hydrodynamics method, which is commonly used for solving shock and blast problems, equivalent explosion simulation models of the laser-induced damage process have been established. Moreover, laser damage experiments combined with time-resolved techniques are performed on KDP surfaces to investigate the impact of laser fluences on the shockwave propagation and the particle ejection speed. We find that the simulation models can predict the laser-induced damage behaviors of the KDP crystal, which verifies the validity of the proposed method.

8.
Anal Chim Acta ; 1091: 69-75, 2019 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-31679576

RESUMO

Nanozymes have emerged as promising alternatives to overcome the high cost and low stability issues of natural enzymes. Particularly, those with peroxidase-like activities have been extensively studied to construct versatile biosensors. In this article, we demonstrate that the modification of the graphitic carbon nitride nanosheets (g-C3N4 nanosheets) by plasmonic gold nanoparticles (AuNPs) greatly enhances its catalytic performance as peroxidase mimetic. In the presence of H2O2, the AuNPs@g-C3N4 nanosheets can catalyze the redox reaction of 3,3',5,5'- tetramethylbenzidine (TMB) to produce a blue color. Based on the observation, a colorimetric sensing method for glucose is further developed with the assistance of glucose oxidase (GOx). The linear range for glucose is from 5 to 100 µmol L-1 (R2 = 0.9967) and the limit of detection (LOD) is 1.2 µmol L-1. The LOD can be further lowered down to 0.75 µmol L-1 by using H2SO4 as termination agent and measuring the absorbance of the yellow product at λ = 451 nm. Moreover, the practical usefulness of AuNPs@g-C3N4 nanosheets as a peroxidase nanozyme for glucose determination in human serum and urine is also demonstrated.

9.
Aging (Albany NY) ; 112019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31683259

RESUMO

This study explored the influence of long non-coding RNA (lncRNA) SNHG14 on α-synuclein (α-syn) expression and Parkinson's disease (PD) pathogenesis. Firstly, we found that the expression level of SNHG14 was elevated in brain tissues of PD mice. In MN9D cells, the rotenone treatment (1µmol/L) enhanced the binding between transcriptional factor SP-1 and SNHG14 promoter, thus promoting SNHG14 expression. Interference of SNHG14 ameliorated the DA neuron injury induced by rotenone. Next, we found an interaction between SNHG14 and miR-133b. Further study showed that miR-133b down-regulated α-syn expression by targeting its 3'-UTR of mRNA and SNHG14 could reverse the negative effect of miR-133b on α-syn expression. Interference of SNHG14 reduced rotenone-induced DA neuron damage through miR-133b in MN9D cells and α-syn was responsible for the protective effect of miR-133b. Similarly, interference of SNHG14 mitigated neuron injury in PD mouse model. All in all, silence of SNHG14 mitigates dopaminergic neuron injury by down-regulating α-syn via targeting miR-133b, which contributes to improving PD.

10.
Molecules ; 24(21)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31683794

RESUMO

The application of natamycin as a natural fungicide in edible coatings is challenging because of its low aqueous solubility. In this study, the natamycin/methyl-ß-cyclodextrin (N/ME-ß-CD) inclusion complex was fabricated and incorporated into waxy corn starch-based coatings for postharvest treatments. The phase solubility of natamycin in the presence of ME-ß-CD at 293.2 K, 303.2 K, and 313.2 K is determined and used to calculate the process thermodynamic parameters. The N/ME-ß-CD inclusion complex was confirmed and characterized by FTIR and 1H NMR spectroscopy. The results indicated that the inclusion complex was formed and the hydrophobic part (C16-C26) of natamycin might be partially inserted into the cavity of ME-ß-CD form the wide rim. The effects of N/ME-ß-CD incorporated starch-based coatings (N/ME-ß-CD S coatings) on postharvest treatments of cherry tomatoes were evaluated in vivo. The N/ME-ß-CD S coatings could reduce weight loss, delay fruit ripening, and inhibit fruit decay caused by Botrytis cinerea in tomato fruit during storage.

11.
Opt Express ; 27(20): 28734-28749, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31684619

RESUMO

Ghost imaging (GI) is a novel imaging technique based on the second-order correlation of light fields. Due to limited number of samplings in practice, traditional GI methods often reconstruct objects with unsatisfactory quality. To improve the imaging results, many reconstruction methods have been developed, yet the reconstruction quality is still fundamentally restricted by the modulated light fields. In this paper, we propose to improve the imaging quality of GI by optimizing the light fields, which is realized via matrix optimization for a learned dictionary incorporating the sparsity prior of objects. A closed-form solution of the sampling matrix, which enables successive sampling, is derived. Through simulation and experimental results, it is shown that the proposed scheme leads to better imaging quality compared to the state-of-the-art optimization methods for light fields, especially at a low sampling rate.

12.
Surg Endosc ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686203

RESUMO

OBJECTIVE: Middle pancreatectomy (MP) is safe and feasible in patients with benign or low-grade malignant tumors located at the neck or proximal body of the pancreas. As a tissue-sparing operation, MP can preserve normal pancreatic function and reduce the risk of postoperative endocrine and exocrine insufficiency. However, the morbidity, especially the postoperative pancreatic fistula (POPF) rate, remains high. A robot-assisted surgical system may provide patients with less trauma; however, there are few reports on robot-assisted middle pancreatectomy (RMP). We describe the experience of RMP at our center to illustrate the learning curve (LC). METHODS: From August 2010 to July 2017, 100 patients underwent RMP in the Pancreatic Disease Center of Shanghai Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine. Patient characteristics, operative outcomes, and oncological outcomes were collected and analyzed. The CUSUM curve was analyzed according to operative time and estimated blood loss (EBL) and was used to describe the LC and identify the flexion points. RESULTS: Among the 100 patients who underwent RMP in our hospital, the mean age was 47.5 ± 14.2 years, and 69 patients were female. From the CUSUM curve, we found two flexion points: cases 12 and 44. After 44 cases, the rate of improvement was much faster. We separated the patients into two groups based on the LC (cases 1-44 and cases 45-100). There were significant improvements in operative time (173.1 ± 44.7 min vs. 137.3 ± 30.1 min, p < 0.001) and EBL (103.4 ± 90.0 ml vs. 69.3 ± 53.9 ml, p = 0.021). The overall POPF rate was 32% (32/100), while the incidence rate of biochemical leakage was 14% (14/100). However, there was no significant difference in the risk of POPF or other complications between the two groups. The postoperative length of stay (LOS) was also not different. The 90-day mortality rate was 1%. From our long-term follow-up, pancreatic function was preserved in most patients, with only three cases of endocrine insufficiency and two cases of exocrine insufficiency. CONCLUSION: RMP was helpful and a good choice for the selected patients. PF was the main complication and has not been improved until now. There were two flexion points in the LC at cases 12 and 44. More cases are needed to gain more experience. A larger sample size and prospective studies are needed to verify the advantage of RMP.

13.
J Biomol Struct Dyn ; : 1-13, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31674290

RESUMO

Cytochrome bcc complex is important for ATP synthesis and cellular activity, as a crucial step in the terminal reduction of oxygen in aerobic electron transport chains. The b subunit of cytochrome bcc complex (QcrB) has been reported as a promising anti-tuberculosis target, with many novel anti-tuberculosis scaffolds reported. However, the 3D structure of mycobacterium tuberculosis (M. tuberculosis) QcrB has not been released, making it hard to understand the interactions between QcrB and its inhibitors as well as to develop novel anti-tuberculosis scaffolds. Herein we built the optimal homology model of M. tuberculosis QcrB using the M. smegmatis QcrB structure as template, which was refined through all-atom molecular dynamics simulation. Then, the binding modes of known inhibitors were predicted through molecular docking method, along with molecular dynamics simulation and binding free energy calculation to verify the accuracy of docking results and stability of the protein-inhibitor complexes. The informative key residues within QcrB site enabled us to perform structure-based virtual library screening to obtain potential M. tuberculosis QcrB inhibitors, which were validated through molecular dynamics simulation and MM-GBSA calculation and analyzed through pharmacokinetic properties prediction. Our research would provide a deeper insight into the interactions between M. tuberculosis QcrB and its inhibitors, which boosts to develop novel therapy against tuberculosis.Communicated by Ramaswamy H. Sarma.

14.
Plant Cell ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31690654

RESUMO

Stalk lodging, which is generally determined by stalk strength, results in considerable yield loss and has become a primary threat to maize yield under high-density planting. However, the molecular genetic basis of maize stalk strength remains unclear, and improvement methods remain inefficient. Here, we combined map-based cloning and association mapping and identified the gene stiff1 underlying a major quantitative trait locus (QTL) for stalk strength in maize. A 27.2-kilobase (kb) insertion of a transposable element was present in the promoter of the stiff1 gene, which encodes an F-box domain. This transposable element insertion repressed the transcription of stiff1, leading to the increased cellulose and lignin contents in the cell wall and consequently greater stalk strength. Furthermore, a new precisely edited allele of stiff1 generated through the CRISPR/Cas9 system created a stronger stalk than the natural control. Nucleotide diversity analysis revealed that the promoter of stiff1 was under strong selection in maize stiff-stalk group. Our cloning of stiff1 represents a significant discovery where a transposable element played an important role in maize improvement. The identification of stiff1 and our newly edited stiff1 allele pave the way for large-scale improvement in maize stalks with high efficiency.

15.
Pathol Res Pract ; : 152691, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31676092

RESUMO

Despite significant advances over the past decades of research, pancreatic cancer (PC) continues to have the worst 5-year survival of any malignancy. Dendritic cells (DCs) are the most potent professional antigen-presenting cells and are involved in the induction and regulation of antitumor immune responses. DC-based immunotherapy has been used in clinical trials for PC. Although safety, efficacy, and immune activation were reported in patients with PC, DC vaccines have not yet fulfilled their promise. Additional strategies for combinatorial approaches aimed to augment and sustain the antitumor specific immune response elicited by DC vaccines are currently being investigated. Here, we will discuss DC vaccination immunotherapies that are currently under preclinical and clinical investigation and potential combination approaches for treating and improving the survival of PC patients.

16.
Food Chem Toxicol ; : 110925, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31676349

RESUMO

Manganese (Mn), an essential micronutrient, has the potential to induce apoptosis. The NT3/TrkC ligand/receptor pair known as part of the classic neurotrophic theory plays a critical role in neuronal survival. However, whether the NT3/TrkC-mediated signaling pathways are involved in Mn-induced apoptosis of cortical neurons remains unknown. The present study was designed to investigate the interactions between NT3/TrkC-mediated signaling pathways and Mn-induced apoptosis in cortical neurons. This study showed that subacute Mn exposure significantly increased the levels of pro-apoptotic Bax while decreasing the levels of anti-apoptotic Bcl 2 in the cortex compared with the corresponding control. Markedly reduced NT3 and TrkC levels along with decreased Ras/MAPK and PI3/Akt signaling in the cortex were observed following subacute Mn exposure. We further found increased levels of Bax, cleaved caspase-3, and the total apoptosis rate, and decreased levels of Bcl2, NT3, TrkC, and Ras/MAPK and PI3/Akt signaling in Mn-treated primary cortical neurons. Pretreatment with hNT3 or Z-VAD-FAM ameliorated Mn-induced apoptosis by increasing the levels of NT3 and TrkC and its Ras/MAPK and PI3/Akt signaling pathways. Taken together, our findings clearly indicate that NT3/TrkC and mediated Ras/MAPK and PI3/Akt signaling pathways play a crucial role in Mn-induced neurotoxicity.

17.
Int Health ; 11(S1): S24-S32, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670821

RESUMO

BACKGROUND: According to the 2017 China National Education Development Statistics Bulletin, there were 14.07 million rural-urban migrant children in the compulsory education stage. The mental health of migrant children in China has drawn increasing attention in research. The objective of this study was to compare subjective well-being, self-esteem, prosocial behaviour and family functioning of migrant children vs local children in Shanghai and to explore their relationship. METHODS: A survey was conducted among 2229 students (9-17 y of age; male 52.0%, female 48.0%) and their parents from grades 4 to 8 in four primary schools and four middle schools in Shanghai in 2016. The sample consisted of 1333 migrant children and 896 urban children in three migrant schools and five public schools. A total of 959 rural-urban migrant children and 374 urban hukou migrant children were recruited. The questionnaire for students included the Personal Well-Being Index - School Children (PWI-SC), Satisfaction with Life Scale (SWLS), Positive and Negative Affect Scale for Children, Rosenberg Self-Esteem Scale, Family APGAR Index and prosocial behaviour domain of the Strengths and Difficulties Questionnaire. In the questionnaire for parents, the Personal Well-Being Index and Social Support Rating Scale were used in addition to the sociodemographic characteristics. These data were analysed using one-way analysis of variance, correlation analysis and multiple linear regression. RESULTS: Rural-urban migrant children reported significantly lower PWI-SC, SWLS, positive affect, prosocial behaviour, self-esteem and Family APGAR Index scores and reported higher negative affect scores than local children (p<0.01). The prevalence rate of abnormal prosocial behaviour among rural-urban migrant children was 10%, which was higher than that of local children (5.9%; p<0.001). Compared with rural-urban migrant children in public schools, the SWLS, prosocial behaviour and Family APGAR Index scores of the children in migrant schools were higher and the prevalence rate of abnormal prosocial behaviour was lower (p<0.01). Multiple linear regression analysis indicated that the self-esteem and Family APGAR Index had predictive effects on subjective well-being and prosocial behaviour scores of rural-urban migrant children. CONCLUSIONS: Rural-urban migrant children are susceptible to mental health problems. Additional public policy and interventions by practitioners are needed to support rural-urban migrant children.

18.
Cancer Med ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31670906

RESUMO

BACKGROUND: Cervical cancer is one of the most common causes of cancer-associated mortality among affected women in the world. At present, treatment with weekly cisplatin plus ionizing radiation (IR) therapy is the standard regimen for cervical cancer, especially for locally advanced cervical cancer. The purpose of this study is to determine whether FEN1 inhibitors could enhance the therapeutic effect of IR therapy. METHODS: Western blot was applied to determine the expression of FEN1- and apoptosis-related proteins. Cell growth inhibition assay and colony formation assay were used to determine the effects of FEN1 inhibitor and IR exposure for Hela cells in vitro. CRISPR technology was used to knockdown FEN1 expression level of 293T cells, and tumor xenograft in nude mice was employed to determine the effects of FEN1 inhibitor and IR exposure on tumor growth in vivo. RESULTS: Our data revealed that FEN1 is overexpressed in HeLa cell and can be upregulated further by IR. We also demonstrated that FEN1 inhibitor enhances IR sensitivity of cervical cancer in vitro and in vivo. CONCLUSION: FEN1 inhibitor SC13 could sensitize radiotherapy of cervical cancer cell.

19.
J Surg Res ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31699537

RESUMO

BACKGROUND: The use of a small circular stapler (CS) has been reported to increase the incidence of benign anastomotic stricture of the intrathoracic anastomosis after esophagectomy, but no study has evaluated the effects of the CS size on cervical esophagogastrostomy. Based on a propensity-matched comparison, the present study was designed to determine whether the perioperative outcomes differ between 21- and 25-mm CSs after minimally invasive esophagectomy with cervical anastomosis. METHODS: From January 2015 to December 2017, 162 patients who received CS cervical esophagogastric anastomosis after minimally invasive esophagectomy for esophageal cancer were identified from our surgical database. A propensity-matched analysis was used to compare the outcomes between the 21- and 25-mm CS groups. Endpoints included anastomotic leak, dysphagia, reflux, stricture, and other major postoperative outcomes within 6 postoperative months. RESULTS: There were 69 and 93 patients in the 21- and 25-mm CS groups, respectively. Propensity matching produced 57 patients in each group. The two groups were not remarkably different in benign anastomotic stricture rate (P = 0.528). All strictures were resolved by balloon dilatation. The 25-mm CS group had a significantly longer operative time in cervical anastomosis than the 21-mm group (P = 0.005). No statistically significant differences in anastomotic leak rates, dysphagia scores, reflux scores, or other postoperative complications were noted between the two groups. CONCLUSIONS: The use of a 21-mm CS in minimally invasive esophagectomy with cervical esophagogastric anastomosis did not result in greater anastomotic stricture as compared with a 25-mm CS. The 21-mm CS was associated with a significantly shorter operative time.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31701991

RESUMO

The purpose of this paper was to examine the effect of microbial fuel cells coupled with solar cells (MFC-SCs). In this study, MFC-SCs were constructed to understand the role of intermittent illumination in electricity generation and sediment remediation based on the sediment microbial fuel cell scenario. Furthermore, the microbial community structure on the anode in the sediment was probed using high-throughput sequencing. We identified that SCs with natural intermittent illumination (12 h per day) can promote the electricity production and nutrient utilization of the sediment of MFCs to the greatest extent, which can help manage solar energy utilization for environmental conversion and control the eutrophication of water bodies.The removal rates of NH3-N, NO3-N, organic matter and TP by the MFC-SC were 46.23% ± 1.06%, 41.50% ± 3.80%, 23.20% ± 1.40% and 24.40 ± 5.50%; in contrast, those of the traditional MFC were 25.10% ± 2.40%, 18.70% ± 4.10%, 14.10% ± 0.90% and 13.00% ± 2.50%, respectively. Meanwhile, the treatment groups in MFC-SCs influenced the species components and microflora structure. The 6329 operational taxonomic units (OTUs) in the control group without solar cells outnumbered those of the treatments of 24 h MFC-SC (5676), 12 h MFC-SC (5664) and 3 h MFC-SC (5592). This can advance the enrichment of dominant bacteria; meanwhile, the microbial process and the mechanisms behind it require further study. These results indicate that MFC-SCs provide a comprehensive method of solar energy utilization and environment remediation.

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