Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 864
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-32173994

RESUMO

G-quadruplex DNA undergoes structural polymorphism, leading challenging around using selectively recognizing probe to accurately detect G-quadruplexes in vivo . Herein, we developed a selectively responsive probe, a tripodal cationic fluorescent probe, NBTE , which showed distinguishable fluorescence lifetime response between all kinds of G-quadruplexes and other DNA topologies, and fluorescence quantum yield enhancing upon G-quadruplexes binding. We determined two NBTE- G-quadruplex complex structures with high fluorescence quantum yield by NMR method. The structures indicated NBTE interacted with G-quadruplexes using three arms through π-π stacking, differing from that with duplex DNA using two arms, which rationalized higher fluorescence quantum yield and lifetime response of NBTE upon G-quadruplexes binding. Based on photon counts of FLIM, we detected the percentage of G-quadruplex DNA in live cells with NBTE , and found G-quadruplex DNA content in cancer cells is ~4 times that in normal cells, suggesting the potential applications of this probe in cancer cells detection.

2.
Int J Cancer ; 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32142166

RESUMO

Chronic inflammation plays an important role in primary liver cancer (PLC) etiology and can be influenced by dietary habits. No prospective study has investigated the association of dietary inflammatory index (DII) with PLC incidence and mortality. Therefore, we used prospective data from the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial to fill this gap. The DII was calculated from a validated 137-item food frequency questionnaire in a cohort of 103,902 individuals. Cox regression was used to estimate hazard ratios (HRs) for PLC incidence, and competing risk regression was used to estimate subdistribution HRs (SHRs) for PLC mortality. Restricted cubic spline regression was employed to identify the potential dose-response pattern. A total of 120 PLC cases and 102 PLC deaths were observed during follow-up. Higher DII scores from food and supplement were found to be associated with higher risks of developing PLC (HRTertile 3 vs. 1 2.05; 95% confidence interval [CI] 1.23-3.41) and death from this disease (SHRTertile 3 vs. 1 1.97; 95% CI 1.13-3.41). Similar results were obtained for DII score from food only. A nonlinear dose-response pattern was identified for the aforementioned associations (all pnonlinearity < 0.05). Overall, a more pro-inflammatory diet, as suggested by higher DII scores, is associated with higher risks of PLC incidence and mortality. These findings indicate that encouraging intake of more anti-inflammatory dietary components and reducing intake of pro-inflammatory components represent an attractive strategy to reduce PLC incidence and mortality.

3.
Adv Mater ; : e1908006, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32166844

RESUMO

Inorganic perovskite single crystals have emerged as promising vapor-phase processable structures for optoelectronic devices. However, because of material lattice mismatch and uncontrolled nucleation, vapor-phase methods have been restricted to random distribution of single crystals that are difficult to perform for integrated device arrays. Herein, an effective strategy to control the vapor-phase growth of high-quality cesium lead bromide perovskite (CsPbBr3 ) microplate arrays with uniform morphology as well as controlled location and size is reported. By introducing perovskite seeds on substrates, intractable lattice mismatches and random nucleation barriers are surpassed, and the epitaxial growth of perovskite crystals is accurately controlled. It is further demonstrated that CsPbBr3 microplate arrays can be monolithically integrated on substrates for the fabrication of high-performance lasers and photodetectors. This strategy provides a facile approach to fabricate high-quality CsPbBr3 microplates with controllable size and location, which offers new opportunities for the scalable production of integrated optoelectronic devices.

4.
Dalton Trans ; 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32196064

RESUMO

Two FeII8L12 cubic metal-organic cages were constructed with semi-rigid ligands and they further self-assembled into supramolecular assemblies with three different porous cavities. The supramolecular assemblies showed synergistic adsorption of I2 and TTF, and their solid state spin-crossover behaviors were influenced by the adsorbed guest molecules.

5.
New Phytol ; 2020 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-32112568

RESUMO

Broad-spectrum resistance is highly preferred in crop breeding programs. Previously, we reported the identification of the broad-spectrum resistance-Digu 1 (bsr-d1) allele from rice Digu. The bsr-d1 allele prevents activation of Bsr-d1 expression by Magnaporthe oryzae infection and degradation of H2 O2 by peroxidases, leading to resistance to M. oryzae. However, it remains unknown whether defense pathways other than H2 O2 burst and peroxidases contribute to the bsr-d1-mediated immunity. Blast resistance was determined in rice leaves by spray and punch inoculations. Target genes of OsMYB30 was identified by one-hybrid assays in yeast and electrophoretic mobility shift assay. Lignin contents were measured by phloroglucinol-HCl staining, and acetyl bromide and thioacidolysis methods. Here, we report the involvement of the OsMYB30 gene in the bsr-d1-mediated blast resistance. Expression of OsMYB30 is induced during M. oryzae infection or when Bsr-d1 is knocked out or downregulated, as occurs in bsr-d1 plants upon infection. We further find that OsMYB30 binds to and activates the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S. This action leads to obvious thickening of sclerenchyma cells near the epidermis, inhibiting M. oryzae penetration at the early stage of infection. Our study reveals novel components required for bsr-d1-mediated resistance and penetration-dependent immunity, and advances our understanding of broad-spectrum disease resistance.

6.
Mar Drugs ; 18(3)2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32111068

RESUMO

Conotoxin-Ac1 and its variant conotoxin-Ac1-O6P, were isolated from the venom duct of Conus achatinus, a fish-hunting cone snail species collected in the Sea of Hainan, China. Conotoxin-Ac1 is linear peptide that contain 15 amino acids. In the present study, we synthesized and structurally and functionally characterized conotoxin-Ac1 as well as 19 variants. Electrophysiological results showed that conotoxin-Ac1 inhibited N-methyl-D-aspartate receptor subunit 2B (NR2B) with an IC50 of 8.22 ± 0.022 µM. Further structure-activity studies of conotoxin-Ac demonstrated that polar amino acid residues were important for modulating its active, and the replacement of N1, O9, E10, and S12 by Ala resulted in a significant decrease in potency to NR2B. °Furthermore, conotoxin-Ac1 and conotoxin-Ac1-O6P were tested in hot-plate and tail-flick assays to measure the potential analgesic activity to an acute thermal stimulus in a dose-dependent manner. Subsequently, the analgesic activity of conotoxin-Ac1 mutants was analyzed by the hot-plate method. The results show that N1, Y2, Y3, E10, N11, S12, and T15 play an important role in the analgesic activity of conotoxin-Ac1. N1 and S12 have significant effects on conotoxin-Ac1 in inhibiting NR2B and analgesic activity. In conclusion, we have discovered that conotoxin-Ac1 is an inhibitor of NMDAR and displays antinociceptive activity.

8.
Sci Total Environ ; 720: 137538, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32143043

RESUMO

Modeling is an important way to assess current and future permafrost spatial distribution and dynamics, especially in data poor areas like the Arctic region. Here, we evaluate a physics-based analytical model, Kudryavtsev's active layer model, which is widely used because it has relatively few data requirements. This model was recently incorporated into a component modeling toolbox, allowing for coupled modeling of permafrost and geomorphic processes over geological timescales. However, systematic quantitative assessment of the influence of its controlling parameters on permafrost temperature and active layer thickness predictions has not been undertaken before. We investigate the sensitivity of the Kudryavtsev's active layer model by Monte Carlo simulations to generate probability distributions for input parameters and compare predictions with a comprehensive benchmark dataset of in-situ permafrost observations over entire Alaska. Predicted permafrost surface temperature is highly dependent on mean annual air temperature (r = 0.78 on average), annual temperature amplitude (-0.41), and winter-averaged snow thickness (0.30). Uncertainty of predicted permafrost temperature is relatively small (RMSE = 1 °C), when air temperature and snow depth are well constrained. Similarly, RMSE between simulated and observed ALT at stations is ~0.08 m. However, under given air temperature and snow conditions, soil water content bias can significantly affect modeled active layer thickness (RMSE = 0.1 m or 40% of the observed active layer thickness). If soil water content has a large bias, improvements in other parameters may not significantly improve the active layer predictions of the Kudryavtsev's model.

9.
Artigo em Inglês | MEDLINE | ID: mdl-32051196

RESUMO

BACKGROUND: Total antioxidant capacity (TAC) reflects an individual's overall antioxidant intake. We sought to clarify whether higher TAC is associated with lower risks of pancreatic cancer incidence and mortality in the U.S. general population. METHODS: A total of 96,018 American adults were identified from the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. A ferric-reducing ability of plasma score was used to reflect an individual's TAC intake from diet and/or supplements. Cox regression was used to calculate hazard ratios (HR) for pancreatic cancer incidence, and competing risk regression was used to calculate subdistribution HRs for pancreatic cancer mortality. Restricted cubic spline regression was used to test nonlinearity. RESULTS: A total of 393 pancreatic cancer cases and 353 pancreatic cancer-related deaths were documented. Total (diet + supplements) TAC was found to be inversely associated with pancreatic cancer incidence (HR quartile 4 vs. quartile 1 = 0.53; 95% confidence interval, 0.39-0.72; P trend = 0.0002) and mortality (subdistribution HR quartile 4 vs. quartile 1 = 0.52; 95% confidence interval 0.38-0.72; P trend = 0.0003) in a nonlinear dose-response manner (all P nonlinearity < 0.01). Similar results were observed for dietary TAC. No association of supplemental TAC with pancreatic cancer incidence and mortality was found. CONCLUSIONS: In the U.S. general population, dietary but not supplemental TAC level is inversely associated with risks of pancreatic cancer incidence and mortality in a nonlinear dose-response pattern. IMPACT: This is the first prospective study indicating that a diet rich in antioxidants may be beneficial in decreasing pancreatic cancer incidence and mortality.

10.
Int J Cancer ; 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32105342

RESUMO

Epidemiological studies on magnesium intake and primary liver cancer (PLC) are scarce, and no prospective studies have examined the associations of magnesium intake with PLC incidence and mortality. We sought to clarify whether higher magnesium intake from diet and supplements was associated with lower risks of PLC incidence and mortality in the US population. Magnesium intake from diet and supplements was evaluated through a food frequency questionnaire in a cohort of 104,025 participants. Cox regression was employed to calculate hazard ratios for PLC incidence and competing risk regression was employed to calculate subdistribution hazard ratios for PLC mortality. Restricted cubic spline regression was employed to test nonlinearity. We documented 116 PLC cases during 1,193,513.5 person-years of follow-up and 100 PLC deaths during 1,198,021.3 person-years of follow-up. Total (diet + supplements) magnesium intake was found to be inversely associated with risks of PLC incidence (hazard ratiotertile 3 vs. 1 : 0.44; 95% confidence interval: 0.24, 0.80; ptrend = 0.0065) and mortality (subdistribution hazard ratiotertile 3 vs. 1 : 0.37; 95% confidence interval: 0.19, 0.71; ptrend = 0.0008). Similar results were obtained for dietary magnesium intake. Nonlinear inverse dose-response associations with PLC incidence and mortality were observed for both total and dietary magnesium intakes (all pnonlinearity < 0.05). In summary, in the US population, a high magnesium intake is associated with decreased risks of PLC incidence and mortality in a nonlinear dose-response manner. These findings support that increasing the consumption of foods rich in magnesium may be beneficial in reducing PLC incidence and mortality.

11.
Artigo em Inglês | MEDLINE | ID: mdl-32073815

RESUMO

The electrochemical conversion of carbon dioxide (CO2) to fuels and chemicals is an opportunity for sustainable energy research that can realize both renewable energy storage and negative carbon cycle feedback. However, the selective generation of multicarbon products is challenging because of the competitive hydrogen evolution reaction (HER) and protonation of the reacting adsorbate. Copper-based materials have been the most commonly studied catalysts for CO2 electroreduction due to their ability to produce a substantial amount of C2 products. Here, we report that a nanodendrite configuration can improve the electrocatalytic performance of Cu catalysts, especially multicarbon product formation, while suppressing HER and methane production. The abundant conductive networks derived from the fractal copper dendritic structures with a high electrochemically active surface area (ECSA) facilitate electron transport and mass transfer, leading to superior kinetics for the formation of multicarbon products from CO2 electroreduction. As a result, approximately 70-120% higher ethylene and 60-220% higher C3 (n-PrOH and propanal) yields with lower onset potentials were produced over Cu nanodendrites compared to the initial Cu particles. This work opens an avenue for promoting CO2 electrochemical reduction to multicarbon products by catalyst configuration modulation.

12.
J Chin Med Assoc ; 2020 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-32101893

RESUMO

Type 2 diabetes has become a major disease burden in 21st century. Both incidence and prevalence of type 2 diabetes have quadrupled between 1980 and 2004 in the whole world. Atherosclerotic cardiovascular disease (ASCVD) is the major complication of type 2 diabetes. The introduction of statins in clinical settings is the first revolution in our battle against ASCVD. Most ASCVDs could be prevented or treated with statins. However, statin failed to reduce chronic kidney diseases (CKD) and heart failure (HF). Owing to a mandate from US Food and Drug Administration in 2008 that every new anti-diabetic drug should be tested in clinical trials to demonstrate its safety, we now have a good opportunity to look for better anti-diabetic drugs not only to decrease blood sugar, but also to decrease CVD or renal disease. Among them, glucagon-like peptide-1 receptor agonists (GLP-1 RAs) and sodium-glucose transport protein 2 inhibitors (SGLT-2 i) are two most extensively studied ones. SGLT-2 inhibitors, in particular, prevent CKD and end-stage renal disease, and prevent HF. In the recent CREDENCE trial, canagliflozin reduced renal endpoints by 34% and end-stage renal disease by 32%. Furthermore, in the recent DAPA-HF trial, dapagliflozin decreased hospitalization for HF/cardiovascular death by 26%, and total death by 17%, in patients with HF with reduced ejection fraction, irrespective of diabetes or non-diabetes. The beneficial effects of SGLT-2 inhibitors in CKD and HF are complementary to the effects of statins. The introduction of SGLT-2 inhibitors in clinical practice is the second revolution in cardiovascular prevention.

13.
Adv Mater ; : e1907661, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32108391

RESUMO

The topological surface states (TSS) in topological insulators (TIs) can exert strong spin-orbit torque (SOT) on adjacent magnetization, offering great potential in implementing energy-efficient magnetic memory devices. However, there are large discrepancies among the reported spin Hall angle values in TIs, and its temperature dependence still remains elusive. Here, the spin Hall angle in a modulation-doped Cr-Bix Sb2- x Te3 (Cr-BST) film is quantitatively determined via both transport and optic approaches, where consistent results are obtained. A large spin Hall angle of ≈90 in the modulation-doped Cr-BST film is demonstrated at 2.5 K, and the spin Hall angle drastically decreases to 0.3-0.5 as the temperature increases. Moreover, by tuning the top TSS carrier concentration, a competition between the top and bottom TSS in contributing to SOT is observed. The above phenomena can account for the large discrepancies among the previously reported spin Hall angle values and reveal the unique role of TSS in generating SOT.

14.
Sci Total Environ ; 715: 137018, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32041003

RESUMO

Antibacterial polymer membranes have been widely used in many fields of our daily life. In this study, porous PA6 membrane with ZnO nanoparticles attaching on to the surface of inner pore walls is prepared. Firstly, SMA (styrene maleic anhydride copolymer) is used to graft onto the surface of ZnO nanoparticle in DMF (dimethylformamide). Then the pre-treated ZnO nanoparticles (ZnO-SMA) are added into SEBS (Styrene-ethylene-butylene-styrene copolymer)/PA6 (60/40 wt/wt) blends with co-continuous morphology. The effects of SMA molecular structure (molecular weight and maleic anhydride content) used for ZnO-SMA nanoparticles on their dispersion states in SEBS/PA6/ZnO-SMA nanocomposites are investigated. When SMA3 (MAH = 8 wt%, Mn = 250,000 g mol-1), which has relatively higher molecular weight and lower MAH content, is used as the pre-treating agent, ZnO-SMA3 nanoparticles tend to be dispersed at the phase interface in SEBS/PA6/ZnO-SMA nanocomposites. However, when SMA2 (MAH = 23 wt%, Mn = 110,000 g mol-1) with relatively lower molecular weight and higher MAH content is used, no ZnO-SMA2 nanoparticles locate at the interface but stay within PA6 phase. Porous PA6 membranes are obtained by selectively etching SEBS phase out with xylene. It can be found that porous PA6 membrane containing ZnO-SMA3 nanoparticles still exhibits much better antibacterial property (R = 3.76) toward S. aureus even at a very low ZnO content (0.5 wt%). This result should be ascribed to almost all the ZnO-SMA3 nanoparticles being exposed to the surface of inner pore walls of PA6 membrane. This work proposes an effective method to prepare porous polymer membrane with functional nanoparticles selectively located at the inner pore walls.

15.
BMC Surg ; 20(1): 12, 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31931809

RESUMO

BACKGROUND: To compare the perioperative outcomes and safety of transperitoneal laparoscopic adrenalectomy with those of retroperitoneal laparoscopic adrenalectomy for patients with pheochromocytoma. METHODS: We searched PubMed, EMBASE and the Cochrane Central Register for studies from 1999 to 2019 to assess the perioperative outcomes and safety of transperitoneal laparoscopic adrenalectomy and the retroperitoneal approach for laparoscopic adrenalectomy in patients with pheochromocytoma. After data extraction and quality assessments, we used RevMan 5.2 to pool the data. RESULTS: Four retrospective studies were obtained in our meta-analysis. Patients who underwent retroperitoneal laparoscopic adrenalectomy were associated with shorter operative time (WMD: 34.91, 95% CI: 27.02 to 42.80, I2 = 15%; p < 0.01), less intraoperative blood loss (WMD: 139.32, 95% CI: 125.38 to 153.26, I2 = 0, p < 0.01), and a shorter hospital stay (WMD: 2, 95% CI: 1.18 to 2.82, I2 = 82%, p < 0.01) than patients who underwent transperitoneal laparoscopic adrenalectomy. No significant differences were found in the complication rate (OR: 1.58, 95% CI: 0.58 to 4.33, I2 = 0; p = 0.38) or in the incidence of hemodynamic crisis (OR: 0.74, 95% CI: 0.19 to 2.94, p = 0.67) between the two groups. CONCLUSION: Retroperitoneal laparoscopic adrenalectomy could achieve better perioperative outcomes than the transperitoneal approach for patients with pheochromocytoma.

16.
Sci Total Environ ; 710: 136293, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31926412

RESUMO

Chitin is the most widely distributed oceanic biomass resources. Its monomer unit, N-acetyl-D-glucosamine (NAG), contains precious atomic nitrogen and represents a potential feedstock for the manufacture of regenerative organic nitrogen chemicals. Herein, the conversion of NAG to the platform chemical, 3-acetamido-5-acetylfuran (3A5AF), catalyzed by amino acid ionic liquids, was investigated. The reaction, catalyzed by a very small amount of glycine chloride ionic liquid without any additives, could yield 43.22% 3A5AF in 10 min. By adding CaCl2, a higher yield up to 52.61% was obtained. This work demonstrated the conversion of chitin biomass to 3A5AF in higher yield without using a boron-based catalyst for the first time. Moreover, the ionic liquid catalyst exhibited excellent recyclability, and afforded 43.22-36.59% yield over during eight cycles. This research provides new and green procedures to convert shellfish fishery waste into value-added platform chemicals.

18.
FASEB J ; 34(1): 706-719, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914642

RESUMO

Anti-Müllerian hormone (Amh) is a peptide factor that is known to regulate sexual differentiation and gonadal function in mammals. Although Amh is also suggested to be associated with cognitive development and function in the postnatal brain, little is known about its expression or direct effects on neuronal activities in the hippocampus. Therefore, we assessed Amh and its receptor expression in the hippocampus of male and female mice using PCR, Western blot, and immunofluorescence staining. While Amh-specific receptor expression was comparable between males and females, mRNA and protein levels of Amh were higher in females than those of males. Electrophysiological recordings on acute hippocampal slices showed that exogenous Amh protein addition increased synaptic transmission and long-term synaptic plasticity at the Cornu Ammonis (CA) 3-CA1 synapses. Amh exposure also increased the excitatory postsynaptic potential at CA1 synapses. Our findings support direct and rapid actions of Amh as a paracrine and/or autocrine factor in regulating hippocampal neuronal activities. Data provide functional evidence of Amh-mediated postsynaptic modulation of synaptic transmission and Amh-regulated long-term synaptic plasticity in the hippocampus. These results suggest a potential role of Amh in learning and memory, and a possible cause of the sex differences in cognitive development and function.

19.
J Cell Mol Med ; 24(2): 1917-1933, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31893573

RESUMO

RNA-binding properties of nucleolin play a fundamental role in regulating cell growth and proliferation. We have previously shown that nucleolin plays an important regulatory role in the phenotypic transformation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (Ang II). In the present study, we aimed to investigate the molecular mechanism of nucleolin-mediated phenotypic transformation of VSMCs induced by Ang II. Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) inhibitors were used to observe the effect of Ang II on phenotypic transformation of VSMCs. The regulatory role of nucleolin in the phenotypic transformation of VSMCs was identified by nucleolin gene mutation, gene overexpression and RNA interference technology. Moreover, we elucidated the molecular mechanism underlying the regulatory effect of nucleolin on phenotypic transformation of VSMCs. EGF and PDGF-BB played an important role in the phenotypic transformation of VSMCs induced by Ang II. Nucleolin exerted a positive regulatory effect on the expression and secretion of EGF and PDGF-BB. In addition, nucleolin could bind to the 5' untranslated region (UTR) of EGF and PDGF-BB mRNA, and such binding up-regulated the stability and expression of EGF and PDGF-BB mRNA, promoting Ang II-induced phenotypic transformation of VSMCs.

20.
J Virol ; 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31969436

RESUMO

Molecular mechanisms of Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation have been studied primarily by measuring the total or average activity of an infected cell population, which often consists of mixture of both non-responding and reactivating cells that in turn contain KSHVs at various stages of replication. Studies on KSHV gene regulation at individual cell level would allow us to better understand the basis for this heterogeneity, and new preventive measures could be developed based on findings from non-responding cells exposed to reactivation stimuli. Here we generated a recombinant reporter-virus that we named "Rainbow-KSHV", which encodes three fluorescence-tagged KSHV proteins (mBFP2-ORF6, mCardinal-ORF52, and mCherry-LANA). Rainbow-KSHV replicated similarly to a prototype reporter-KSHV, KSHVr.219 and wild type BAC16 virus. Live imaging revealed unsynchronized initiation of reactivation and KSHV replication with diverse kinetics between individual cells. Cell fractionation revealed temporal gene regulation, in which early lytic genes expression was terminated in late protein expressing cells. Finally, isolation of fluorescence positive cells from non-responders increased dynamic ranges of downstream experiments for 10-fold. This study thus demonstrates a tool to examine heterogenic responses of KSHV reactivation for deeper understanding of KSHV replication.IMPORTANCE Sensitivity and resolution of molecular analysis are often compromised by the use of techniques that measure the ensemble average of large cell populations. Having a research tool to nondestructively identify the KSHV replication stage in an infected cell would not only allow us to effectively isolate cells of interest from cell populations, but also enable more precise sample selection for advanced single cell analysis. We prepared a recombinant KSHV that can report on its replication stage in host cells by differential fluorescence emission. Consistent with previous host gene expression studies, our experiments reveal the highly heterogenic nature of KSHV replication/gene expression at individual cell levels. The utilization of newly developed reporter-KSHV and initial characterization of KSHV replication in single cells are presented.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA