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1.
Plant Sci ; 290: 110295, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31779907

RESUMO

Among the phosphofructokinase B-type carbohydrate kinase (PCK) family proteins, only few proteins, like the FRUCTOKINASE-LIKE 1 and 2, have been functionally characterized in regulation of chloroplast development. Here, we report the involvement of a PCK protein PFKB1 in chloroplast development by identification of a new rice mutant, revertible early yellowing Kitaake 2 [rey(k2)]. The mutant rey(k2) shows yellow leaf phenotype, reduced photosynthetic pigments, and retarded chloroplast development during early stages of seedlings, but gradually recovered at later stages. The phenotype of rey(k2) is resulted from the disruption of the PFKB1 protein. The Pfkb1 gene is ubiquitously expressed, and its protein is mainly targeted to the chloroplast and, in some cells, to the nucleus. In addition, the PFKB1 protein possesses phosphofructokinase activity in vitro. The rey(k2) mutant affects RNA levels of chloroplast-associated genes. In particular, the nuclear-encoded RNA polymerase (NEP)-dependent genes are expressed at a sustained high level in rey(k2) even after turning green, indicating that PFKB1 is essential for suppressing the expression of NEP-dependent genes. Taken together, our study suggests that PFKB1 functions as a novel regulator indispensable for early chloroplast development, at least partly by regulating chloroplast-associated genes.

2.
J Hazard Mater ; 383: 121189, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31541958

RESUMO

Phthalates have been reported to affect the function and growth of thyroid. However, there is little data on the effect of phthalates on thyroid oncogenesis. Here we explored the associations between phthalates exposure and the risks of thyroid cancer and benign nodule. We sex-matched 144 thyroid cancer, 138 benign nodule patients and 144 healthy adults from Wuhan, China. Eight phthalate metabolites in spot urine samples were quantified using high-performance liquid chromatography and tandem mass spectrometry. The associations of creatinine-corrected urinary phthalate metabolites with the risks of thyroid cancer and benign nodule were assessed using multivariable logistic regression models. We found that urinary monomethyl phthalate (MMP), mono(2-ethyl-5hydroxyhexyl) phthalate (MEHHP) and mono(2-ethylhexyl) phthalate (MEHP) associated with increased risks of thyroid cancer and nodule, with adjusted odds ratios (ORs) ranging from 1.74 to 4.78 comparing the extreme tertiles, and urinary monobutyl phthalate (MBP) was associated with decreased risks of thyroid cancer and benign nodule (all P for trends < 0.05). Male-specific positive associations of urinary monoethyl phthalate (MEP) with thyroid cancer and nodule as well as urinary mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) with thyroid cancer were also observed. Our results suggest that exposure to certain phthalates may contribute to increased risks of thyroid cancer and benign nodule.

3.
Heart Vessels ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31786644

RESUMO

The femoral approach with the Needle's Eye Snare (NES) is often used for bailout after failure of the superior approach for transvenous lead extraction (TLE). The safety and efficacy of the NES as a first-line tool for TLE remain unclear. The medical records of patients who underwent TLE via the femoral approach utilizing the NES from May 2014 to June 2019 in Peking University People's Hospital were retrospectively reviewed. Nine hundred and eighty-five leads were extracted in 492 patients (369 men; mean age 72.8 ± 29.0 years). The median (range) number of leads extracted per patient was 2 (1-6). The mean indwelling time of all extracted leads was 112.6 ± 52.0 months. The complete procedure success rate, clinical success rate, and failure rate were 94.1% (463/492), 97.8% (481/492), and 1.1% (11/492), respectively. Major complications including death occurred in nine patients (1.9%), of whom eight developed cardiac tamponade. Among these eight patients, emergency pericardiocentesis followed by rescue surgical repair if necessary was successful in 6 (75.0%) and failed in 2 (25.0%). No significant differences were found in the clinical success rate or major complications rate between patients with pacemakers and implantable cardioverter defibrillators, or between patients with infected and uninfected leads. A femoral approach with the NES is safe and effective for TLE of both pacing and defibrillator leads and could be considered a first-line approach. Cardiac tamponade was the most frequent cardiovascular complication. A strategy of emergency pericardiocentesis followed by a rescue surgical approach seems to be reasonable technique to treat a cardiac tamponade.

4.
Sensors (Basel) ; 19(23)2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31783659

RESUMO

Permutation entropy (PE), as one of the powerful complexity measures for analyzing time series, has advantages of easy implementation and high efficiency. In order to improve the performance of PE, some improved PE methods have been proposed through introducing amplitude information and distance information in recent years. Weighted-permutation entropy (W-PE) weight each arrangement pattern by using variance information, which has good robustness and stability in the case of high noise level and can extract complexity information from data with spike feature or abrupt amplitude change. Dispersion entropy (DE) introduces amplitude information by using the normal cumulative distribution function (NCDF); it not only can detect the change of simultaneous frequency and amplitude, but also is superior to the PE method in distinguishing different data sets. Reverse permutation entropy (RPE) is defined as the distance to white noise in the opposite trend with PE and W-PE, which has high stability for time series with varying lengths. To further improve the performance of PE, we propose a new complexity measure for analyzing time series, and term it as reverse dispersion entropy (RDE). RDE takes PE as its theoretical basis and combines the advantages of DE and RPE by introducing amplitude information and distance information. Simulation experiments were carried out on simulated and sensor signals, including mutation signal detection under different parameters, noise robustness testing, stability testing under different signal-to-noise ratios (SNRs), and distinguishing real data for different kinds of ships and faults. The experimental results show, compared with PE, W-PE, RPE, and DE, that RDE has better performance in detecting abrupt signal and noise robustness testing, and has better stability for simulated and sensor signal. Moreover, it also shows higher distinguishing ability than the other four kinds of PE for sensor signals.

5.
Med Sci Monit ; 25: 9207-9215, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793519

RESUMO

BACKGROUND Cystatin C is a protease inhibitor that is increased in the serum of patients with chronic kidney disease (CKD) and is associated with an increased risk of developing cardiovascular disease (CVD). This study aimed to evaluate the association between serum levels of cystatin C and arterial stiffness, associated with dyslipidemia, obesity, and increased pulse pressure, in middle-aged and elderly individuals without CKD in a population in China. MATERIAL AND METHODS A cross-sectional population-based study included 1,138 patients aged ≥40 years without CKD, defined as an estimated glomerular filtration rate measured by serum creatinine (eGFRSCr) ≥60 ml/min/1.73 m². Study participants provided clinical details, including height and weight, and blood samples for serum measurements of cystatin C and lipid profiles and completed a clinical questionnaire. Pulse pressure was calculated as the mean systolic pressure (SBP) minus the diastolic pressure (DBP). Data underwent multivariate logistic regression analysis. RESULTS An increase in serum levels of cystatin C was associated with an increased risk of arterial stiffness. Each standard deviation in the increase of cystatin C resulted in a 22% increased risk of dyslipidemia, a 27% increased risk of obesity, and a 24% increased risk of increased pulse pressure, after adjusting for confounders. These associations were further confirmed in a sensitivity analysis by excluding participants with hypertension, diabetes, and patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). CONCLUSIONS In middle-aged and elderly individuals without CKD, arterial stiffness determined by obesity, dyslipidemia and increased pulse pressure, was significantly associated with increased serum levels of cystatin C.

6.
Neurosurg Rev ; 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31707577

RESUMO

The purpose of this study was to evaluate the natural history of patients with these heterogeneous aneurysms to provide guidance for their treatment. This retrospective analysis was performed at a single institution and included 137 patients with complex intracranial aneurysms who underwent a natural history evaluation. Among the 115 patients who underwent bypass surgery, stroke (n = 39, 33.9%) was the most common clinical symptom followed by progressively severe headaches (n = 25, 21.7%). Of the 104 patients with follow-up information, 87 (83.7%) returned to a normal life within a mean follow-up of 4.17 ± 2.09 years. Ten deaths (9.6%) occurred after a mean of 1.3 ± 0.9 years. Among the 22 patients who selected nonsurgical treatment, mass effect (n = 9, 40.9%) was the most common clinical presentation, and 14 deaths (63.6%) occurred after a mean of 3.3 ± 2.5 years. The modified Rankin scale (mRS) scores of 5 survivors (5/22, 22.7%) progressed from 0-2 at initial presentation to 3-4. Bypass surgical treatment for these aneurysms appears to be effective and can achieve good clinical outcomes without additional limitations related to individual aneurysms despite the impact of recent endovascular techniques on vascular surgery.

7.
Aging (Albany NY) ; 11(21): 9442-9460, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694982

RESUMO

Transforming growth factor-ß1 (TGFß1)-induced differentiation into and the activation of myofibroblasts have been regarded as critical events in benign prostatic hyperplasia (BPH); however, the underlying mechanisms of BPH pathogenesis remain unclear. Microarray profiling, STRING analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation, and Gene Ontology (GO) enrichment analysis were performed to confirm the candidate genes and long non-coding RNA (lncRNAs) related to BPH. Collagen Type III (COL3A1) was significantly upregulated by TGFß1 in prostate stromal cells (PrSCs) and might be involved in DNM3OS function in myofibroblasts upon TGFß1 stimulation. Upon TGFß1 stimulation, COL3A1 protein was decreased by DNM3OS silencing. miR-29a and miR-29b could directly bind to the DNM3OS and COL3A1 3' untranslated region (UTR)s to negatively regulate their expression, and by serving as a competing endogenous RNAs (ceRNA), DNM3OS competed with COL3A1 for miR-29a/29b binding, therefore counteracting miR-29a/29b-mediated COL3A1 suppression. The effect of DNM3OS silencing on ECM components and TGFß1 downstream signaling was similar to that of the TGFß1 inhibitor SB431542. miR-361 could target DNM3OS and TGFß1; DNM3OS competed for miR-361 binding to counteract miR-361-mediated TGFß1 suppression. In conclusion, we identified DNM3OS as a specifically-upregulated lncRNA upon TGFß1 stimulation in PrSCs; by serving as a ceRNA for the miR-29a/29b cluster and miR-361, DNM3OS eliminated miRNA-mediated suppression of COL3A1 and TGFß1, thereby promoting TGFß1-induced PrSC transformation into myofibroblasts.

8.
Aging (Albany NY) ; 11(22): 10454-10467, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31760385

RESUMO

It is well recognized that type 2 diabetes mellitus (T2DM) is an age-related metabolic disease, emerging gradually as a major global health burden that has gained public attention. Meanwhile, increasing attention is paid to the crucial role of gut microbiota in the pathogenesis and therapeutic mechanisms of metabolic disorders, especially T2DM. In this study, we used C57 BL/KS db/db male mice as a T2DM murine model. We found that the ß-diversity and relative abundances of gut bacteria were obviously altered in db/db mice, associated with a significant increase in Verrucomicrobia at six levels (phylum, class, order, etc.) and family S24-7 and a significant decrease in Bacteroidaceae at family, genus, and species levels, as well as Prevotellaceae at family and genus levels. Furthermore, fecal bacteria from db/db and m/m mice transplanted into pseudo-germ-free mice showed a significant change in the metabolic parameters, including the body weight, fasting blood glucose, fluid and food intake, and alterations in the composition of the gut microbiota. Taken together, these findings suggest that abnormalities in the composition of the gut microbiota might contribute to the development of T2DM and that potential therapeutic strategies improving gut microbiota might provide beneficial effects for individuals with T2DM and age-related glucose intolerance.

9.
J Diabetes ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31747113

RESUMO

BACKGROUND: To comprehensive evaluated the association between husband smoking amount and wives' dysglycemia status. METHODS: We conducted a cross-sectional study which recruited 12 023 714 reproductive couples participated in the National Free Pre-Pregnancy Checkups Project (NFPCP) between 2015 and 2016 in China. Multivariable logistic models were used to estimate OR and 95% CI. Subgroup analysis and stratified analysis were further performed to investigate potential effect modification. RESULTS: Comparing to the neither-smoker group, the multivariable-adjusted ORs for DM among women was 1.09 (95 CI: 1.07-1.10), 1.04 (95% CI: 0.87-1.23) and 1.28 (95% CI: 1.17-1.41) in the husband-smoker, wife-smoker and both-smokers group, respectively. Increased risk of DM was also observed for women whose husband smokes 1 to 10; 11 to 20 and over 20 cigarettes per day (OR:1.06; 95% CI: 1.04-1.07; OR:1.13; 95% CI:1.11-1.15; OR: 1.25; 95% CI: 1.17-1.31). The prevalence of wives' DM showed significant linear trends with husband smoking amount (p for trend<0.001). The similar growth tendency had also obtained between husband smoking amount and IFG prevalence with OR 1.04 (95% CI:1.04-1.05); 1.05 (95% CI: 1.04-1.06); 1.09 (95% CI: 1.06-1.11) for 1-10, 11-20 and over 20 cigarettes per day, respectively. The relationship between husband smoking amount and women IFG/DM prevalence appear to be modified by BMI. CONCLUSIONS: This study showed significant relationships between husband smoking and wives' risk of IFG/DM, which result exist in both categorical and dose-response manner. This association is modified by BMI. Family-oriented smoking interventions may both reduce husband active smoking and wives risk of DM. This article is protected by copyright. All rights reserved.

10.
Med Sci Monit ; 25: 8928-8939, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31761897

RESUMO

BACKGROUND Bladder cancer is a very common urological cancer globally, and cisplatin- or gemcitabine-based chemotherapy is essential for advanced bladder cancer patients. Many patients with bladder cancer have a relatively poor response to chemotherapy, leading to failure of clinical treatment. We mined the GSE77883 GEO dataset, identifying FoxR2 as being a significantly upregulated gene in T24 chemoresistant cells. Herein, we assessed how FoxR2 functions in bladder cancer cell chemoresistance. MATERIAL AND METHODS Cisplatin-resistant T24 (T24/DDP) cells were constructed by administering increasing concentrations of cisplatin, and differences in expression of FoxR2 were examined in T24/DDP and T24 cells. FoxR2 loss- and gain-of-function cells models were established in T24/DDP and T24 cells, respectively. Cell survival, clone formation, cell cycle, and cell apoptosis were assessed, and the MYC pathway was verified. RESULTS FoxR2 was significantly upregulated in T24/DDP cells compared to T24 cells. Knockdown of FoxR2 in T24/DDP cells, survival rate, and clone formation were decreased, G1/S phase transition was suppressed, and cell apoptosis was promoted. These results were reversed by restoration of FoxR2 levels in T24 cells. We found that FoxR2 knockdown enhanced sensitivity to cisplatin, whereas MYC overexpression antagonized chemosensitivity in T24/DDP cells. CONCLUSIONS FoxR2 knockdown decreases chemoresistance to cisplatin via the MYC pathway in bladder cancer cells, and this may be a target for overcoming chemoresistance in bladder cancer.

11.
Brain Res ; : 146536, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31676226

RESUMO

The stroke incidence in menopausal women was abruptly increased combining with much worse stoke outcomes. Estrogen replacement therapy has the potential to become a new neuroprotective strategy against stroke. But the adverse oncogenic events extremely limited its clinical application. As estrogen receptor (ER) ß is seldom expressed in the female reproductive organs, selective activation of ERß is a promising alternative therapy. However, the role and mechanism of ERß in stroke neuroprotection remain largely unknown. In this study, we investigated the effects of activating ERß on microglia and astrocyte activation and NF-κB mediated neuroinflammatory injury induced by middle cerebral artery occlusion and reperfusion (MCAO-R) or oxygen and glucose deprivation and reperfusion (OGD-R). We found that 8 mg/kg DPN (ERß-specific agonist) replacement therapy (3 weeks) to the ovariectomized (OVX) mice significantly reduced ischemia injury and alleviated microglia and astrocyte activation, and markedly inhibited the expression of NF-κB and proinflammatory cytokines (TNF-α, IL-1ß, and IL-6). Moreover, pretreatment (72 h) with 10 nM DPN to the cell line of microglia (N9) or astrocyte (MA1800) significantly increased the cell viability and decreased the cell apoptosis and damage after OGD-R injury, and significantly inhibited the expression of NF-κB and proinflammatory cytokines. These results concluded that DPN replacement treatment alleviated the cerebral ischemia-reperfusion injury via inhibiting the activation of microglia and astrocyte and NF-κB mediated neruoinflammation. As ERß agonist have only minor effects in classic estrogen target tissues, we propose that selective activating ERß is a promising therapy to suppress stroke neuroinflammatory injury in menopausal women.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31729139

RESUMO

Singlet fission (SF) holds the potential to boost the maximum power conversion efficiency of photovoltaic devices. Internal conversion (IC) has been considered as one of the major competitive deactivation pathways to transform excitation energy into heat. In this contribution, using time-resolved spectroscopy and theoretical calculation, we demonstrate that, instead of conventional IC pathway, an unexpected intramolecular singlet fission (iSF) process is responsible for excited state deactivation in isoindigo derivatives. The 1TT state could form at ultrafast rate and nearly quantitatively in solution. In solid films, the slipped stacked intermolecular packing of a thiophene-functionalized derivative leads to efficient triplet pair separation, giving rise to an overall triplet yield of 181 %. Our work not only enrich the pool of iSF-capable materials, but also contribute to a better understanding of the iSF mechanism, which could be relevant for designing new SF sensitizers.

13.
J Clin Microbiol ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31723011

RESUMO

Pregenomic RNA (pgRNA) is a direct transcription product of HBV covalently closed circular DNA (cccDNA) and plays important roles in viral genome amplification and replication. This study was designed to investigate whether serum pgRNA is a strong alternative marker for reflecting HBV cccDNA levels and to analyze the correlation between serum pgRNA, serum HBV DNA and hepatitis B surface antigen (HBsAg). A total of 400 HBV-infected patients who received nucleos(t)ide analogs (NAs) therapy with different clinical outcomes were involved in this research. Case group included asymptomatic hepatitis B virus carrier (ASC), chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC), 100 patients for each group. The results showed that the level of HBV pgRNA had significant difference in these 4 groups. Serum pgRNA levels correlated well with serum HBV DNA levels and HBsAg levels (HBV pgRNA levels vs. HBV DNA levels, r=0.58, P<0.001; HBV pgRNA levels vs. HBsAg levels, r=0.47, P<0.001). In addition, we focused on the 108 HBV-infected patients with HBV DNA <500 IU/ml, it was surprising to find that 17.57% (13/74) of HBV pgRNA can be detected even when HBV DNA level was below 20 IU/ml. In conclusion, HBV pgRNA levels in serum can be a surrogate marker for intrahepatic HBV cccDNA compared with serum HBV DNA and HBsAg. The detection of serum HBV pgRNA levels may provide a reference for clinical monitoring of cccDNA levels and the selection of appropriate timing for discontinuing antiviral therapy, especially when HBV DNA levels are below the detection limit.

14.
Viruses ; 11(11)2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31752437

RESUMO

The coastal sediments were considered to contain diverse phages playing important roles in driving biogeochemical cycles based on genetic analysis. However, till now, benthic phages in coastal sediments were very rarely isolated, which largely limits our understanding of their biological characteristics. Here, we describe a novel lytic phage (named Shewanella phage S0112) isolated from the coastal sediments of the Yellow Sea infecting a sediment bacterium of the genus Shewanella. The phage has a very high replication capability, with the burst size of ca. 1170 phage particles per infected cell, which is 5-10 times higher than that of most phages isolated before. Meanwhile, the latent period of this phage is relatively longer, which might ensure adequate time for phage replication. The phage has a double-stranded DNA genome comprising 62,286 bp with 102 ORFs, ca. 60% of which are functionally unknown. The expression products of 16 ORF genes, mainly structural proteins, were identified by LC-MS/MS analysis. Besides the general DNA metabolism and structure assembly genes in the phage genome, there is a cluster of auxiliary metabolic genes that may be involved in 7-cyano-7-deazaguanine (preQ0) biosynthesis. Meanwhile, a pyrophosphohydrolase (MazG) gene being considered as a regulator of programmed cell death or involving in host stringer responses is inserted in this gene cluster. Comparative genomic and phylogenetic analysis both revealed a great novelty of phage S0112. This study represents the first report of a benthic phage infecting Shewanella, which also sheds light on the phage-host interactions in coastal sediments.

15.
Artigo em Inglês | MEDLINE | ID: mdl-31772008

RESUMO

The environment has a strong influence on a population's evolutionary dynamics. Driven by both intrinsic and external factors, the environment is subject to continual change in nature. To capture an ever-changing environment, we consider a model of evolutionary dynamics with game transitions, where individuals' behaviors together with the games that they play in one time step influence the games to be played in the next time step. Within this model, we study the evolution of cooperation in structured populations and find a simple rule: Weak selection favors cooperation over defection if the ratio of the benefit provided by an altruistic behavior, b, to the corresponding cost, c, exceeds [Formula: see text], where k is the average number of neighbors of an individual and [Formula: see text] captures the effects of the game transitions. Even if cooperation cannot be favored in each individual game, allowing for a transition to a relatively valuable game after mutual cooperation and to a less valuable game after defection can result in a favorable outcome for cooperation. In particular, small variations in different games being played can promote cooperation markedly. Our results suggest that simple game transitions can serve as a mechanism for supporting prosocial behaviors in highly connected populations.

16.
Exp Ther Med ; 18(6): 4528, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31772639

RESUMO

[This retracts the article DOI: 10.3892/etm.2017.4342.].

17.
Nat Commun ; 10(1): 5262, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31748525

RESUMO

Foreign body reaction (FBR) to implanted biomaterials and medical devices is common and can compromise the function of implants or cause complications. For example, in cell encapsulation, cellular overgrowth (CO) and fibrosis around the cellular constructs can reduce the mass transfer of oxygen, nutrients and metabolic wastes, undermining cell function and leading to transplant failure. Therefore, materials that mitigate FBR or CO will have broad applications in biomedicine. Here we report a group of zwitterionic, sulfobetaine (SB) and carboxybetaine (CB) modifications of alginates that reproducibly mitigate the CO of implanted alginate microcapsules in mice, dogs and pigs. Using the modified alginates (SB-alginates), we also demonstrate improved outcome of islet encapsulation in a chemically-induced diabetic mouse model. These zwitterion-modified alginates may contribute to the development of cell encapsulation therapies for type 1 diabetes and other hormone-deficient diseases.

18.
Adv Mater ; : e1905135, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31709667

RESUMO

Cell replacement therapy is emerging as a promising treatment platform for many endocrine disorders and hormone deficiency diseases. The survival of cells within delivery devices is, however, often limited due to low oxygen levels in common transplantation sites. Additionally, replacing implanted devices at the end of the graft lifetime is often unfeasible and, where possible, generally requires invasive surgical procedures. Here, the design and testing of a modular transcutaneous biphasic (BP) cell delivery device that provides enhanced and unlimited oxygen supply by direct contact with the atmosphere is presented. Critically, the cell delivery unit is demountable from the fixed components of the device, allowing for surgery-free refilling of the therapeutic cells. Mass transfer studies show significantly improved performance of the BP device in comparison to subcutaneous controls. The device is also tested for islet encapsulation in an immunocompetent diabetes rodent model. Robust cell survival and diabetes correction is observed following a rat-to-mouse xenograft. Lastly, nonsurgical cell refilling is demonstrated in dogs. These studies show the feasibility of this novel device for cell replacement therapies.

19.
World Neurosurg ; 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31655237

RESUMO

BACKGROUND: A blood blister aneurysm (BBA) is an abnormal bulge at the nonbranching point of a vessel. However, the optimal treatment strategy for this formidable disorder remains unknown. The aim of this study was to evaluate the safety and validity of using a direct microsurgical repair technique in BBAs. METHODS: Direct microsuturing of aneurysms was performed with nylon thread in 7 patients from 2014 to 2018. Postoperative angiography was utilized to confirm the obliteration of the aneurysms and the absence of stenosis of the parent artery. Neurological function was assessed by the modified Rankin scale (mRS). RESULTS: Two male and 5 female patients with a mean age of 43.7 years (range, 29-62 years) were assessed. Subarachnoid hemorrhage (SAH) occurred in 6 patients, including 4 patients with Hunt-Hess grade II and 2 patients with grade III. BBAs of the internal carotid artery (ICA) were observed in three patients, BBAs of the MCA trunk were observed in two patients and a BBA of the anterior communicating artery (ACoA) was observed in one patient. One BBA of the ACoA in one patient was detected incidentally during the resection of a craniopharyngioma. All BBAs were closed with blood-tight sutures via standard frontotemporal craniotomies. Postoperatively, all BBAs were completely eliminated from the circulation without stenosis of the sutured parent vessel. CONCLUSIONS: The proposed microsuture technique appears to be a safe, cost-effective, durable treatment for BBAs in the anterior circulation and should be a part of the arsenal of neurosurgical practitioners who treat anterior circulation BBAs.

20.
Sensors (Basel) ; 19(20)2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31658745

RESUMO

Commercial soil moisture sensors have been widely applied into the measurement of soil moisture content. However, the accuracy of such sensors varies due to the employed techniques and working conditions. In this study, the temperature impact on the soil moisture sensor reading was firstly analyzed. Next, a pioneer study on the data-driven calibration of soil moisture sensor was investigated considering the impacts of temperature. Different data-driven models including the multivariate adaptive regression splines and the Gaussian process regression were applied into the development of the calibration method. To verify the efficacy of the proposed methods, tests on four commercial soil moisture sensors were conducted; these sensors belong to the frequency domain reflection (FDR) type. The numerical results demonstrate that the proposed methods can greatly improve the measurement accuracy for the investigated sensors.

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