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1.
J Biomed Mater Res B Appl Biomater ; 108(1): 117-127, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30912304

RESUMO

Developing a small-diameter vascular graft with a satisfactory performance in terms of mechanical and biological properties remains a challenging issue because of comprehensive requirements from clinical applications. Polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels exhibit many desirable characteristics for small-diameter vascular grafts because of their tunable mechanical properties, especially high compliance. However, poor cells adhesion hinders their application for endothelialization in situ. Therefore, in the present work, polydopamine (PDA) and tetrapeptide Arg-Glu-Asp-Val (REDV) were used to functionalize the hydrogels surface and improve cells adhesion. A series of characterizations were systematically conducted to examine the applicability of coated hydrogels to small-diameter vascular grafts. Results showed that bare and coated hydrogels have appropriate structural stability, and no significant differences in tensile properties could be found after being coated with PDA or PDA-REDV. The hydrophilicity of the hydrogels decreased with the coatings of PDA and especially PDA-REDV to improve protein adsorption, porcine iliac artery endothelial cells (PIECs) adhesion, viability, proliferation, and spreading on the hydrogels. Lower hemolysis percentages and higher blood clotting index values were attained for the hydrogels, suggesting their satisfactory hemocompatibility. Overall, the present work provided insights into the development of a novel hydrogel-based small-diameter vascular graft. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:117-127, 2020.

2.
Anal Chim Acta ; 1094: 136-141, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31761040

RESUMO

Magnetic ion-selective colorimetric microspheres based on surface modification of polystyrene beads (0.8 µm diameter) are reported for the first time. The common components of ion-selective optode sensing (chromoionophore, ion-exchanger and ionophore) and magnetic nanoparticles are adsorbed onto the surface of the polystyrene particles using a simple mixed solvent method. The average diameter of the magnetic microspheres is evaluated by dynamic light scattering as 0.79 ±â€¯0.06 µm. The reversible microsensors are circulated by flow and accumulated at a single spot by an applied magnet to become observable by digital camera. Hue signals are extracted from the recorded images to quantify the ratio of protonated and deprotonated form of the chromoionophore, which is the basis for optode response. The resulting magnetic microsensors respond to K+ with excellent selectivity over the range of 10-6 M to 10-2 M and a response time of t99 < 2.6±0.5 min above 10-5 M. The use of solvatochromic dyes as pH independent transducers was not successful in this application.

3.
Water Res ; 168: 115093, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31606557

RESUMO

Hydroxylamine showed an outstanding performance on enhancing the oxidation of pollutants in Fe(II) involved advanced oxidation processes, while the detailed reaction schemes have not been fully revealed. Specific functions of hydroxylamine in the oxidation of benzoic acid with Fe(II)/persulfate (PDS) system were explored. With the addition of hydroxylamine, degradation kinetics of benzoic acid deviated from both two-stage kinetics and pseudo first order kinetics, but could be interpreted well with binomial regression analysis. Degradation rate constant (kobs) of benzoic acid was calculated and showed the same variation trend with [hydroxylamine][Fe(III)]2/([Fe(II)][H+])2, the value of which was changed during reaction processes. A detailed kinetic model for simulating the degradation profile of benzoic acid with hydroxylamine acceleration was proposed for the first time and indicated that interactions of hydroxylamine and Fe(III) were fast equilibrium reactions, which was a dominant factor influencing the oxidation kinetics of benzoic acid in Fe(II)/hydroxylamine/PDS system. Comparative study showed that when 1.4 mM of ascorbic acid was added into Fe(II)/PDS system, degradation kinetics of benzoic acid was similar to that enhanced by hydroxylamine. However, when 0.6 mM or 1.0 mM of ascorbic acid was added, oxidation kinetics still presented as the two-stage profile. Kinetic simulations indicated that Fe(II) was produced slower from Fe(III)-ascorbic acid complexes than that with hydroxylamine, which caused the difference in oxidation kinetics. This study could improve our understanding about the effect of hydroxylamine and other reductants in promoting pollutants elimination in Fe(II)/PDS system.


Assuntos
Compostos Ferrosos , Poluentes Químicos da Água , Compostos Férricos , Hidroxilamina , Hidroxilaminas , Cinética , Oxirredução
4.
J Cell Physiol ; 235(1): 114-127, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31347175

RESUMO

Myosin phosphatase-Rho interacting protein (p116Rip ) was originally found as a RhoA-binding protein. Subsequent studies by us and others revealed that p116Rip facilitates myosin light chain phosphatase (MLCP) activity through direct and indirect manners. However, it is unclear how p116Rip regulates myosin phosphatase activity in cells. To elucidate the role of p116Rip in cellular contractile processes, we suppressed the expression of p116Rip by RNA interference in human airway smooth muscle cells (HASMCs). We found that knockdown of p116Rip in HASMCs led to increased di-phosphorylated MLC (pMLC), that is phosphorylation at both Ser19 and Thr18. This was because of a change in the interaction between MLCP and myosin, but not an alteration of RhoA/ROCK signaling. Attenuation of Zipper-interacting protein kinase (ZIPK) abolished the increase in di-pMLC, suggesting that ZIPK is involved in this process. Moreover, suppression of p116Rip expression in HASMCs substantially increased the histamine-induced collagen gel contraction. We also found that expression of the p116Rip was decreased in the airway smooth muscle tissue from asthmatic patients compared with that from non-asthmatic patients, suggesting a potential role of p116Rip expression in asthma pathogenesis.

5.
Nanotechnology ; 31(2): 025601, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31518998

RESUMO

In this study, a novel photocatalyst composed of N-doped TiO2 (N-TiO2) and (Ca, Y)F2:Yb3+, Tm3+ was prepared by simple dealloying followed by a hydrothermal method. The composite exhibits a homogeneous nanoporous structure consisting of large quantities of the spindle-like N-doped TiO2 nanorods, on which the (Ca, Y)F2:Yb3+, Tm3+ particles with a diameter of around 5 nm are uniformly dispersed. In addition, morphology and property of the N-TiO2 can be controlled by adjusting the dealloying period. Results show that a short immersion time leads to a small size, large surface area and low band gap. As a result, the N-TiO2/(Ca, Y)F2:Yb3+, Tm3+ composite after dealloying for 48 h (TiO2-48-C) exhibits higher degradation rates (65.6% for 10 h irradiation by 980 nm NIR) than others after dealloying for 60 h (TiO2-60-C) and 72 h (TiO2-72-C), indicating its excellent potential for practical applications.

6.
Food Chem Toxicol ; : 111012, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794802

RESUMO

In order to improve the bioavailability of Oryza sativa L. anthocyanins, we fabricated Oryza sativa L. anthocyanins-Hohenbuehelia serotina polysaccharides (OSA-HSP) complex and investigated its anti-proliferation activities taking into account its changes along simulated gastrointestinal digestion in vitro. Results showed that OSA mainly composed by delphinidin, cyanidin, petunidin, malvidin and their derivatives was combined with HSP through electrostatic interaction. OSA-HSP complex belonged to non-crystalline substance, and had compact and laminar structural characteristics. Under simulated gastrointestinal digestion, OSA-HSP complex significantly prevented the degradation of anthocyanins, and presented sustain release effect. However, the anti-proliferation activities of OSA-HSP complex digested by different gastrointestinal process were remarkably changed, especially after small intestinal digestion. HeLa cells treated with OSA-HSP complex exhibited pro-apoptosis characteristics by triggering endogenous mitochondrial apoptosis pathway through activating the expressions of Bax, cytochrome c and Caspase-3 as well as inhibiting the expression of Bcl-2. These findings provided new insight to improve the bioavailability of anthocyanins in functional foods and tumor therapy.

7.
Pathol Res Pract ; : 152765, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31796335

RESUMO

OBJECTIVES: To analyze the clinicopathologic feature, diagnosis and differential diagnosis of undifferentiated myxoid lipoblastoma in infant. METHODS: The study included 2 cases of undifferentiated myxoid lipoblastoma in infant according to the molecular genetic diagnosis. The relevant clinicopathologic feature was investigated. RESULTS: We describe 2 cases of undifferentiated myxoid lipoblastoma in infant. The both large circumscribed masses are located in deep soft tissue. Unlike most lipoblastoma, lobulated appearance was not obvious in one case and completely absent in another. The both cases presented prominent myxoid change with a plexiform vascular pattern. There were some spindle-shaped or stellate mesenchymal cells, while no any mature adipocytes. The initial suggestion of case 1 was myxoid liposarcoma, and case 2 was aggressive angiomyxoma. However, few S-100 positive lipoblasts suggested the origin of the tumor. FISH analysis using a PLAG1 break apart probe confirmed a PLAG1 rearrangment. The final diagnosis was undifferentiated myxoid lipoblastoma. CONCLUSIONS: The undifferentiated myxoid lipoblastoma is a very rare tumor in infant. Histologically, prominent myxoid change, a plexiform vascular pattern and lacking of mature adipocytes make it indistinguishable from myxoid liposarcoma, PMMTI and aggressive angiomyxoma. The S-100 positive lipoblasts and genetic rearrangement of PLAG1 helps in confirming the diagnosis. Even if there were no mature adipocytes, myxoid lipoblastoma was still a diagnosis that can not be ignored in myxoid tumors in children.

8.
Plant Physiol Biochem ; 146: 392-402, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31794899

RESUMO

Tea cultivars with leaf color variation have attracted increasing attention in tea production and research due to their unusual appearances and appealing flavors. However, the molecular mechanism underlying this variation is little known due to the unavailability of genetic transformation and a highly complex genome. Here, a natural tea plant mutant producing pale green branches (pgb) was discovered and characterized. Ultrastructural and biochemical analyses showed that the leaves of the pgb mutant had defective chloroplast structure and significantly lower pigment content than the normal control. Comprehensive expression detection of chloroplast-development-related genes further indicated that a significant downregulation of CsGLKs in the pgb mutant likely caused the chloroplast defect. Transcriptome analyses and polyphenolic compound determination highlighted a tight correlation between photosynthesis and secondary metabolite biosynthesis in tea plant. These results provide useful information illuminating the mechanism of chloroplast development and leaf color variation in tea plant.

9.
FEMS Microbiol Lett ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31800029

RESUMO

A nuclease from Yersinia enterocolitica subsp. palearctica (Nucyep) is a newly found thermostable nonspecific nuclease. The heat-resisting ability of this nuclease would be extremely useful in biological research or pharmaceutical production. However, the application of this nuclease is limited because of its poor yield. This research aimed to improve Nucyep productivity by producing a novel genetically engineered Escherichia coli and optimizing the production procedures. After 4 h of induction by lactose, the new genetically engineered E. coli can express a substantial amount of Nucyep in the form of inclusion bodies. The yield was approximately 0.3 g of inclusion bodies in 1 g of bacteria pellets. The inclusion bodies were extracted by sonication and solubilized in an 8 M urea buffer. Protein renaturation was successfully achieved by dilution method. Pure enzyme was obtained after subjecting to anion exchange. The Nucyep showed its nonspecific and heat resistant properties as previous report. Through quantification method, its activity was determined to be 1.3 × 106 K.U./mg. These results can serve as a reference for increasing Nucyep production.

10.
Cerebrovasc Dis ; : 1-7, 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31786566

RESUMO

BACKGROUND: Hemorrhagic transformation (HT) is a frequent complication of acute ischemic stroke (AIS). Red blood cell distribution width (RDW) is a cost-effective parameter associated with incidence and prognosis of cerebrovascular diseases. The purpose of this study was to assess whether RDW is associated with HT in AIS patients. METHODS: AIS patients within 24 h from stroke onset between January 1, 2014, and December 31, 2018, were consecutively enrolled. Blood samples were collected. The primary outcome was HT, which was diagnosed by follow-up brain image and classified into hemorrhagic infarct (HI) and parenchymal hematoma (PH). Multivariate logistic regression analysis was performed to determine the relationship between RDW and HT as well as its subtypes. Potential effect modifier was identified by stratified logistic regression analysis. RESULTS: Among the included 1383 patients, 220 (15.9%) developed HT (HI in 103 and PH in 117). Elevated RDW levels were associated with an increased risk of HT when 2 extreme tertiles were compared (OR 1.60, 95% CI 1.04-2.44, p = 0.031). The risk of HT increased stepwise across RDW tertiles (p for trend = 0.042). RDW significantly correlated with HI rather than PH. The association between RDW and HT could be modified by reperfusion therapy (p for interaction = 0.010), with no significant association between RDW and HT among patients underwent reperfusion therapy. CONCLUSIONS: Elevated RDW level was related to increased risk of HT among AIS patients without reperfusion therapy.

11.
J Glob Health ; 9(2): 020601, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31788232

RESUMO

Background: Various hypertension predictive models have been developed worldwide; however, there is no existing predictive model for hypertension among Chinese rural populations. Methods: This is a 6-year population-based prospective cohort in rural areas of China. Data was collected in 2007-2008 (baseline survey) and 2013-2014 (follow-up survey) from 8319 participants ranging in age from 35 to 74 years old. Specified gender hypertension predictive models were established based on multivariate Cox regression, Artificial Neural Network (ANN), Naive Bayes Classifier (NBC), and Classification and Regression Tree (CART) in the training set. External validation was conducted in the testing set. The estimated models were assessed by discrimination and calibration, respectively. Results: During the follow-up period, 432 men and 604 women developed hypertension in the training set. Assessment for established models in men suggested men office-based model (M1) was better than others. C-index of M1 model in the testing set was 0.771 (95% confidence Interval (CI) = 0.750, 0.791), and calibration χ2 = 6.3057 (P = 0.7090). In women, women office-based model (W1) and ANN were better than the other models assessed. The C-indexes for the W1 model and the ANN model in the testing set were 0.765 (95% CI = 0.746, 0.783) and 0.756 (95% CI = 0.737, 0.775) and the calibrations χ2 were 6.7832 (P = 0.1478) and 4.7447 (P = 0.3145), respectively. Conclusions: Not all machine-learning models performed better than the traditional Cox regression models. The W1 and ANN models for women and M1 model for men have better predictive performance which could potentially be recommended for predicting hypertension risk among rural populations.

12.
Int Immunopharmacol ; : 106037, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31784402

RESUMO

Drug-induced liver injury is an important adverse drug reaction. Due to the lack of specificity in clinical symptoms and pathological features, there are still no reliable diagnostic biomarkers, so drug-induced liver injury is a diagnosis of exclusion. The article reviews the relevant advances in the association between novel human leukocyte antigen gene polymorphisms and drug-induced liver injury in order to identify potential biomarkers and provide a new method for the prediction and diagnosis of drug-induced liver injury. Henceforth, while studying the association between them, it will also need that the large sample and prospective studies to gain supporting evidence to implement translational application, so as to improve the safety and effectiveness of medication and achieve individualized treatment.

13.
Nat Chem ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792385

RESUMO

Live-cell fluorescence nanoscopy is a powerful tool to study cellular biology on a molecular scale, yet its use is held back by the paucity of suitable fluorescent probes. Fluorescent probes based on regular fluorophores usually suffer from a low cell permeability and an unspecific background signal. Here we report a general strategy to transform regular fluorophores into fluorogenic probes with an excellent cell permeability and a low unspecific background signal. Conversion of a carboxyl group found in rhodamines and related fluorophores into an electron-deficient amide does not affect the spectroscopic properties of the fluorophore, but allows us to rationally tune the dynamic equilibrium between two different forms: a fluorescent zwitterion and a non-fluorescent, cell-permeable spirolactam. Furthermore, the equilibrium generally shifts towards the fluorescent form when the probe binds to its cellular targets. The resulting increase in fluorescence can be up to 1,000-fold. Using this simple design principle, we created fluorogenic probes in various colours for different cellular targets for wash-free, multicolour, live-cell nanoscopy.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31803631

RESUMO

Family with sequence similarity 96 member A (FAM96A) is an evolutionarily conserved intracellular protein that is involved in the maturation of the Fe/S protein, iron regulatory protein 1 (IRP1), and the mitochondria-related apoptosis of gastrointestinal stromal tumor cells. In this study, we used a mouse model of chemically induced colitis to investigate the physiological role of FAM96A in intestinal homeostasis and inflammation. At baseline, colons from Fam96a -/- mice exhibited microbial dysbiosis, dysregulated epithelial cell turnover, an increased number of goblet cells, and disordered tight junctions with functional deficits affecting intestinal permeability. After cohousing, the differences between wild-type and Fam96a -/- colons were abrogated, suggesting that FAM96A affects colonic epithelial cells in a microbiota-dependent manner. Fam96a deficiency in mice resulted in increased susceptibility to dextran sulfate sodium (DSS)-induced colitis. Importantly, the colitogenic activity of Fam96a -/- intestinal microbiota was transferable to wild-type littermate mice via fecal microbial transplantation (FMT), leading to exacerbation of DSS-induced colitis. Taken together, our data indicate that FAM96A helps to maintain colonic homeostasis and protect against DSS-induced colitis by preventing gut microbial dysbiosis. This study used gene knockout animals to help to understand the in vivo effects of the Fam96a gene for the first time and provides new evidence regarding host-microbiota interactions.

15.
Cancer Sci ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804030

RESUMO

Patients with lower-risk myelodysplastic syndromes (LR-MDS) as defined by the International Prognostic Scoring System (IPSS) have more favorable prognosis in general, but significant inter-individual heterogeneity exists. In this study, we examined the molecular profile of 15 MDS-relevant genes in 159 patients with LR-MDS using next-generation sequencing. In univariate COX regression, shorter overall survival (OS) was associated with mutation status of ASXL1 (P=0.001), RUNX1 (P=0.031), EZH2 (P=0.049), TP53 (P=0.016), SRSF2 (P=0.046), JAK2 (P=0.040), and IDH2 (P=0.035). We also found significantly shorter OS in patients with an adjusted TET2 variant allele frequency (VAF) ≥18% versus those with either an adjusted TET2 VAF <18% or without TET2 mutations (median: 20.4 vs. 47.8 months; P=0.020; HR=2.183, 95%CI: 1.129-4.224). After adjustment for the IPSS, shorter OS was associated with mutation status of ASXL1 (P<0.001; HR=4.306, 95%CI: 2.144-8.650), TP53 (P=0.004; HR=4.863, 95%CI: 1.662-14.230) and JAK2 (P=0.002; HR=5.466, 95%CI: 1.848-16.169), as well as adjusted TET2 VAF ≥18% (P=0.008; HR=2.492, 95%CI: 1.273-4.876). Also, OS was increasingly shorter as the number of mutational factors increased (P<0.001). A novel prognostic scoring system incorporating the presence/absence of the 4 independent mutational factors into the IPSS further stratified LR-MDS patients into three prognostically different groups (P<0.001). The newly developed scoring system re-defined 10.1% (16/159) of patients as higher-risk group, who could not be predicted by the currently prognostic models. In conclusion, integration of the IPSS with mutation status/burden of certain MDS-relevant genes may improve the prognostication of patients with LR-MDS and could help identify those with worse-than-expected prognosis for more aggressive treatment.

16.
Medicine (Baltimore) ; 98(49): e18125, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804322

RESUMO

BACKGROUND: Functional constipation (FC) is a prevalent clinical disease that affects a considerable proportion of the population of all ages. Persistent FC significantly reduces quality of life and influences physical and emotional well-being, as well as consumes many substantial healthcare resources. Acupuncture originates from Traditional Chinese Medicine (TCM), and emerging evidence of several randomized controlled trials (RCTs) published suggest that acupuncture has positive effects for FC. Since 2019, several new results of high quality RCTs about acupuncture treatment for FC have been published. Thus a systematic review will be designed to appraise the effectiveness and safety of acupuncture for improvement of FC in patients based on high quality RCTs. METHODS: We carried out a rigorous literature search in English and Chinese electronic database from inception to present. Two reviewers will identify relevant studies, extract and manage trial information, and then assess the risk of bias in included studies by the Cochrane risk of bias assessment tool. Only high quality RCTs will be included. Data will be synthesized by either fixed-effects or random-effects model regarding to a heterogeneity test. The primary outcome measurement will be the change from baseline in mean complete spontaneous bowel movements and stool form. The secondary outcomes involved disappearance rate of symptoms, proportion of responders, mean transit time, health-related quality of life, and safety of intervention. Meta-analysis will be performed by using Cochrane's RevMan software. RESULTS: This systematic review will summarize high quality clinical evidence to assess and appraise the effectiveness and safety of acupuncture treatment for FC patient. EXPECTED CONCLUSION: This systematic review and meta-analysis will provide evidence to determine whether acupuncture treatment is an effective and safe therapy for the prevention and treatment of FC compared with medication treatment.

17.
Arch Psychiatr Nurs ; 33(6): 167-176, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31753224

RESUMO

PURPOSE: The purpose of this study is to investigate the current situation of violence on primary caregivers of people with severe mental illness outside the hospital and the cognition of primary caregivers about violence among mental patients. METHODS: A self-designed questionnaire was used to investigate the primary caregivers (N = 208) of people with severe mental illness in a psychiatric hospital in Beijing. RESULTS: The incidence rate of violence suffered by primary caregivers was 74.03%, of which 61.54% had experienced verbal attacks, 54.33% had experienced threats, and 45.19% experienced physical attacks. Physical violence, mainly by unarmed attacks, has resulted in soft tissue injury and pain in the majority of caregivers. Multiple admission times, a lower educational level, single in marital status and involuntary hospitalizations were risk factors; growing older was protective factor. The causes of violence were dominated by mental symptoms in 120 cases (57.69%). Tolerance and avoidance were the coping styles of most caregivers adopted after violence, accounting for 51.44%. Furthermore, most of primary caregivers have limited knowledge of violence and adopted an attitude of pessimism towards patients' violence. CONCLUSIONS: It was reported that violence was suffered by primary caregivers of persons with severe mental illness outside the hospital. The study indicated that formulating reasonable nursing intervention, providing health education as well as organizing training towards violence of patients are required to play an important role in effectively preventing and reducing the violence among the people with severe mental illness in China. More information and support needs to be obtained to help caregivers fulfill their duty of care outside the hospital.

18.
Sci Rep ; 9(1): 17645, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31754184

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

19.
Opt Express ; 27(22): 31235-31245, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684359

RESUMO

Doppler wind lidar is an effective tool for wind detection with high temporal and spatial resolution. However, precise wind profile measurement under rainy conditions is a challenge, due to the interfering signals from raindrop reflections. In this work, a compact all-fiber coherent Doppler lidar (CDL) at working wavelength of 1.5 µm is applied for simultaneous wind and precipitation detection. The performance of the lidar is validated by comparison with the weather balloons. Thanks to the ability of precise spectrum measurement, both aerosol and rainfall signals can be detected by the CDL under rainy conditions. The spectrum width is used to identify the precipitation events, during which the two-peak Doppler spectrum is observed. The spectrum is fitted by a two-component Gaussian model and two velocities are obtained. By using the velocity-azimuth display (VAD) scanning technique, wind speed and rainfall speed are simultaneously retrieved. The false detection probability of wind speed in the rainy conditions is thus reduced.

20.
Biotechnol Lett ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31720977

RESUMO

Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi. Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications. The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.

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