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1.
J Hazard Mater ; 401: 123372, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32645542

RESUMO

To investigate the deleterious ecological effects of cyanobacteria on submerged macrophytes, this study investigated the effects of different concentrations of fresh cyanobacteria (FC) and cyanobacteria decomposition solution (CDS) on an experimental group of submerged macrophytes (Vallisneria natans (Lour.) Hara and Myriophyllum verticillatum Linn.). The results showed that FC and CDS not only lead to decrease in biomass and significant changes in enzyme activity and chlorophyll content in tissue, but also affected the permeability of cell membranes. The extent of damage was in the order CDS > FC, and the comprehensive stress resistance of Vallisneria natans (2.994) was more than that of Myriophyllum verticillatum (2.895). In addition, semi-permeable membranes can reduce plant damage by FC and CDS, but cannot completely prevent it. FC and CDS mainly affected the relative distribution of microbial genera on the surface of aquatic plants (p < 0.05). Furthermore, CDS caused irreversible damage to plant cells and induced programmed cell death (PCD) of plants to accelerate their decline. Therefore, FC and CDS may be one of the main reasons for the decline in submerged vegetation. This study provides a scientific basis for evaluating the harmful effects of cyanobacteria on submerged macrophytes.

2.
J Hazard Mater ; 401: 123390, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32659584

RESUMO

Monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs), phthalate metabolites (mPAEs), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the urine of school children aged 8-11 years from Shenzhen, China were measured in order to investigate oxidative stress damage from co-exposure to PAHs and PAEs. The concentrations of OH-PAHs and mPAEs in urine were 0.36-36.5 (median: 3.86) and 9.48-1609 (median: 240) ng/mL respectively. Gender and age did not influence urinary concentrations of ΣOH-PAHs and ΣmPAEs, but geographical variations (i.e., urban versus suburban) were observed. Levels of 8-OHdG were positively correlated with urinary OH-PAHs and mPAEs, with correlation coefficients (r) varying between 0.160 and 0.365 (p < 0.05). OH-PAHs made a greater contribution to oxidative DNA damage than mPAEs when these two types of pollutants were present at the same concentrations. Human health risks were assessed using the hazard quotient and the hazard index for the cumulative risk of a complex of chemicals. The results demonstrated that risks from PAHs could be neglected, but that 29.5 % of school children may be subject to obvious health risks from PAEs, especially diethylhexyl phthalate.

3.
Theranostics ; 10(24): 11302-11323, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33042284

RESUMO

Background: Activation of the thermogenic program in white and brown adipocytes presents a promising avenue for increasing energy expenditure during the treatment of obesity. The endogenous mechanism for promoting thermogenesis in brown adipocytes or browning in white adipocytes has indicated that the gut microbiota is a crucial regulator of the host energy balance. However, whether the effects of the therapeutic intervention-induced modulation of the gut microbiota on adipocyte browning involved the regulation of leptin remains unclear. Method: The adipose features were analyzed by body composition analysis, infrared camera observations, transmission electron microscopy and H&E staining. The gene and protein expression in adipose tissue were detected by qRT-PCR, immunoblotting, immunohistochemistry and immunofluorescence staining. The gut microbiome signature was identified by 16S rRNA gene amplicon sequencing, and both mice with high-fat diet-induced obesity (DIO) and mice with antibiotics-induced microbiome depletion were subjected to fecal microbiota transplantation. Results: Treatment with Panax notoginseng saponins (PNS) shaped the murine gut microbiome by increasing the abundances of Akkermansia muciniphila and Parabacteroides distasonis, and as a result, DIO mice harbored a distal gut microbiota with a significantly increased capacity to reduce host adiposity. The PNS-induced modulation of the gut microbiota in DIO mice could increase brown adipose tissue (BAT) thermogenesis and beige adipocyte reconstruction by activating the leptin-AMPK/STAT3 signaling pathway, which results in the promotion of energy expenditure. Leptin has an essential influence on the anti-obesity effects of PNS. In cases of leptin deficiency, the PNS-induced modulation of the gut microbiota exerts negative effects on thermogenesis and browning in white adipose tissue (WAT), which indicates that PNS fail to reduce obesity in leptin gene-deficient mice. The PNS-induced modulation of the gut microbiota exerted a minimal effect on DIO mice with antibiotic-induced microbiome depletion, which confirmed the correlation between altered gut microbiota and the remodeling of adipose tissues in DIO mice. The direct influence of leptin on browning via the AMPKα/STAT3 signaling pathway in C3H101/2 cells supported our in vivo results that signalling through the leptin-AMPK/STAT3 pathway induced by the PNS-modulated gut microbiota was involved in beige adipocyte reconstruction. Conclusion: Our results revealed that leptin signaling is critical for alterations in microbiota-fat crosstalk and provide promising avenues for therapeutic intervention in the treatment of obesity.

4.
Plants (Basel) ; 9(10)2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33036140

RESUMO

Brachypodium distachyon, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. Senescence associated gene 101 (SAG101) has been studied extensively in Arabidopsis. SAG101 is one of the important regulators of plant immunity. However, no homologous genes of AtSAG101 were found in B. distachyon. In this study, the AtSAG101 gene was transformed into B. distachyon. Three transgenic plant lines containing the AtSAG101 gene were confirmed by PCR and GUS gene activity. There were fewer Puccinia brachypodii urediospores in the AtSAG101-overexpressing plants compared to wild type plants. P. brachypodii biomass was obviously decreased in AtSAG101 transgenic plants. The length of infection hyphae and infection unit areas of P. brachypodii were significantly limited in transgenic plants. Moreover, there were small lesions in AtSAG101 transgenic plants challenged by Magnaporthe oryzae. Salicylic acid accumulation was significantly increased, which led to elevated pathogenesis-related gene expression in transgenic B. distachyon inoculated by P. brachypodii or M. oryzae compared to wild type plants. These results were consistent with infected phenotypes. Overexpression of AtSAG101 in B. distachyon caused resistance to M. oryzae and P. brachypodii. These results suggest that AtSAG101 could regulate plant resistance in B. distachyon.

5.
Chemosphere ; 264(Pt 1): 128500, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33035954

RESUMO

Carbide slag may pollute the environment because it is difficult to handle. In this paper, carbide slag without pretreatment served as the new source of calcium and was added to bituminous coal for gasification experiments to realize waste utilization. The gasification experiment after adding carbide slag to bituminous coal enhances H2 production, which reduced the activation energy of the gasification reaction. The results show that the catalytic effect on steam gasification was evident when the carbide slag was added to Mongolian bituminous coal. The coal char at reaction temperature was prepared and characterized by X-ray diffraction (XRD), Raman, Scanning electron microscope (SEM), Transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), and FT-IR spectroscopy. The carbon structure evolution and calcium structure changes of coal char under reaction temperature were studied, and the influence of coal char structure changes on gasification performance was analyzed. The results show that in the coal char added with carbide slag, the oxygen-containing functional groups generated by the polycondensation reaction interacted with calcium to form a calcium-oxygen-carbon complex. The existence of this structure not only leads to the highly uniform dispersion of CaO in the char but also hinders the graphitization process of the char. Highly dispersed CaO and disordered carbon structure significantly improved the reactivity of bituminous coal steam gasification. Si and Al in the bituminous coal affected the dispersion of Ca during steam gasification.

6.
Carbohydr Res ; 498: 108168, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33049653

RESUMO

The degree of acetylation (DA) is an essential parameter for chitin and its derivatives, which determines the chemical and physical properties of the polymers. As a consequene, fast and accurate technique to determine DA is widely required when developing the relating materials. Herein, an improved quantitative SSNMR method of rQCPZRC, based on the cross polarization reciprocity relation, was discussed and employed for DA testing. Three chitin/chitosan samples were chosen to evaluate the performance of rQCPZRC. In comparison with quantitative DP and optimized contact time CP methods, rQCPZRC is revealed as an accurate and reliable DA testing method with relative percentage errors of less than 5%. Moreover, the experimental time of rQCPZRC for each sample is 5.5 h, notably shorter than DP of 36-85 h. Thus, our work suggests rQCPZRC as a tool for DA testing, which is capable to accomplish with high accuracy and efficiency.

7.
Sci Total Environ ; 755(Pt 2): 142633, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33075688

RESUMO

Ecological civilization construction is an essential means of achieve sustainable development in China. It promotes not only the decoupling of environmental degradation from economic development, but additionally the coupling of positive ecological development with economic development. Presently, most of the research on ecological civilization focuses on its indices and evaluation methods. However, there exist some gaps such as the use of incomplete scientific indicators, and insufficient practice caused by inadequate sample size. In this study, we first take the evaluation framework for ecological civilization pilot areas combined with academic research to construct a comprehensive framework and indicator system. Second, we calculate the Coupling Coordination Degree (CCD) for each of the pilot areas based on the entropy weight and identify typical industries that promote the coupling of ecology and economy. Third, we use the Relative Development Coefficient (RDC) to measure the development of ecology and economy between 2014 and 2019, and study the different kinds of development models for cities. Results of the study found that the regional economy is highly positive correlated with CCD, indicating a mutually reinforcing relationship between economic development and ecological development. Further, the RDC reveals that the level of urban ecological development is relatively higher at the stage of decoupling and coordination with economic system. Finally, strategic emerging industries are a common element in pilot areas with a high level of ecological development, as they offer higher economic output without the ecological degradation associated with traditional industries.

8.
Bioorg Med Chem Lett ; : 127614, 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33080352

RESUMO

Congenital disorders of glycosylation (CDG) are a growing group diseases that result from defects in genes involved in glycan biosynthesis pathways. One tetrasaccharide, i.e., Neu5Ac-α2, 6-Gal-ß1, 4-GlcNAc-ß1, 4-GlcNAc, was recently reported as the biomarker of ALG1-CDG, the disease caused by ALG1 deficiency. To develop a novel diagnostic method for ALG1-CDG, chemo-enzymatic synthesis of the tetrasaccharide biomarker linked to phytanyl phosphate and the biomarker's immune stimulation were investigated in this study. The immunization study using liposomes bearing phytanyl-linked tetrasaccharide revealed that they stimulated a moderate immune response. The induced antibody showed strong binding specificity for the ALG1-CDG biomarker, indicating its potential in medical applications.

9.
NPJ Genom Med ; 5: 43, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33083012

RESUMO

Tissue-specific transcription factors are frequently inactivated in cancer. To fully dissect the heterogeneity of such tumor suppressor events requires single-cell resolution, yet this is challenging because of the high dropout rate. Here we propose a simple yet effective computational strategy called SCIRA to infer regulatory activity of tissue-specific transcription factors at single-cell resolution and use this tool to identify tumor suppressor events in single-cell RNA-Seq cancer studies. We demonstrate that tissue-specific transcription factors are preferentially inactivated in the corresponding cancer cells, suggesting that these are driver events. For many known or suspected tumor suppressors, SCIRA predicts inactivation in single cancer cells where differential expression does not, indicating that SCIRA improves the sensitivity to detect changes in regulatory activity. We identify NKX2-1 and TBX4 inactivation as early tumor suppressor events in normal non-ciliated lung epithelial cells from smokers. In summary, SCIRA can help chart the heterogeneity of tumor suppressor events at single-cell resolution.

10.
J Affect Disord ; 279: 75-82, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33039777

RESUMO

News media workers are in stressful and competitive professions susceptible to negative emotions. Although the relationship between negative emotions and job burnout has been evidenced in some professional fields, it has not yet been examined in the population of news media workers. Far few studies have revealed the mediating and moderating mechanism between negative emotions and job burnout. Based on Resource Allocation Theory and Theory of Compassion Fatigue, this study examined (a) the mediating role of rumination in the relationship between negative emotions and job burnout, (b) the moderating role of empathy in the direct and indirect effect of negative emotions on job burnout, and (c) the moderating role of gender in the direct effect of negative emotions on rumination. Two hundred and eleven Chinese news media workers filled out questionnaires regarding negative emotions, job burnout, rumination, and empathy. The results indicated that negative emotions were positively correlated with job burnout. Mediation analysis revealed that rumination partially mediated the association between negative emotions and job burnout. Moderated mediation analysis further indicated that empathy moderated the effect of negative emotions on rumination, with a high level of empathy strengthening the association between negative emotions and rumination; gender moderated the effect of negative emotions on rumination, with the association between negative emotions and rumination was stronger among males than females. These findings encourage to design psychological interventions to reduce rumination and express proper empathy in the face of stress or negative affective status to avoid job burnout among news media workers.

11.
J Stroke Cerebrovasc Dis ; 29(11): 105221, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33066883

RESUMO

INTRODUCTION: Cerebral small-vessel disease (CSVD) is an extensive cerebrovascular disease associated with many poor outcomes. Previous studies have shown that brachial-ankle pulse wave velocity (baPWV) is related to various neuroimaging signatures, but its association with the total CSVD burden remains unknown. We aimed to explore whether baPWV is related to the total CSVD score and to establish a cutoff for detecting the presence and severity of CSVD, which may guide clinical preventive measures. METHODS: We retrospectively selected 684 neurologically healthy participants to explore correlations between baPWV and the total CSVD score and each of its components (lacunes, white matter hyperintensity (WMH), perivascular space (PVS), and cerebral microbleeds (CMBs)). Subsequently, we established two receiver operating characteristic (ROC) curves to study the effectiveness of baPWV in predicting CSVD (scores 1-4) and severe CSVD (scores 3-4). RESULTS: The median baPWV was 13.16 m/s, which increased significantly with increasing scores (0-4). BaPWV was significantly higher among persons with each component of the total CSVD score than among those without any components. Multivariable ordinal logistic regression analyses showed that a one-unit (m/s) change in baPWV significantly increased the total CSVD score by 0.012. The optimal baPWV cutoffs for detecting CSVD and severe CSVD were 13.12 m/s and 15.63 m/s, respectively. CONCLUSIONS: BaPWV was positively correlated with the total CSVD score, suggesting that baPWV measurement is a useful method for early diagnosis of CSVD, which may contribute to preventing and controlling CSVD progression in the general population of China.

12.
Front Endocrinol (Lausanne) ; 11: 581002, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33071985

RESUMO

Local site-specific differences between bones in different regions of the skeleton account for their different properties and functions. To identify mechanisms behind these differences, we have performed a cross-species study comparing RNA transcriptomes of cranial and tibial osteocytes, from bones with very different primary functions and physiological responses, collected from the same individual mouse, rat, and rhesus macaque. Bioinformatic analysis was performed to identify 32 genes changed in the same direction between sites and shared across all three species. Several well-established key genes in bone growth and remodeling were upregulated in the tibias of all three species (BMP7, DKK1, FGF1, FRZB, SOST). Many of them associate or crosstalk with the Wnt signaling pathway. These results suggest Wnt signaling-related candidates for different control of regulatory mechanisms in bone homeostasis in the skull and tibia and indicate a different balance between genetically determined structure and feedback mechanisms to strains induced by mechanical loading at the different sites.

13.
Medicine (Baltimore) ; 99(42): e22754, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33080741

RESUMO

BACKGROUND: Heart failure (HF) is the terminal stage of various common cardiovascular diseases with quite a frequent readmission and a high mortality rate, and brings heavy financial burdens to families and society. Oral Chinese patent medicine (CPM) has been widely applied in the treatment of HF in China because of its simplicity, cheapness, convenience, and high efficiency. However, due to the large number and broad clinical selectivity of oral CPMs, there is a lack of uniformity and clinical application standardization. To choose more effective and safe medicine among so many oral CPMs is particularly essential for further improving the therapeutic effect. In this study, the efficacy and safety of different oral CPMs will be compared by a network meta-analysis (NMA), and the best CPM will be selected for the treatment of HF. METHODS: According to the search strategy, 4 English and 4 Chinese databases will be searched from the construction of the library to July 31, 2020. The NMA will include clinical randomized controlled trials (RCTs) of different oral CPMs in HF treatment. The methodological quality is assessed according to the bias risk assessment tool of Cochrane. The Bayesian NMA is performed by Aggregate Data Drug Information System (ADDIS), and the results are visualized using Stata 15.0 software. The GRADE approach is used to assess the quality of evidence and recommendation intensity. RESULTS: The NMA will identify the best oral CPM in the complementary treatment of HF. A peer-reviewed journal will publish the results of the study. CONCLUSION: This study can provide reliable evidence for the efficacy and safety of oral CPMs in the treatment of HF, and help decision-makers and patients to select more effective and safer oral CPM. PROTOCOL REGISTRATION NUMBER: INPLASY202090053.

14.
J Food Biochem ; : e13497, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33029846

RESUMO

In this work, a comparison study was conducted on the contents of total flavonoids and hyperoside in different polarity extracts of Fructus rosae laxae (FRL). The lipid-lowering effect and mechanism of FRL ethyl acetate extract (FRLE) on the lipid accumulation model of Bel-7402 cells in vitro were studied. The results showed that the contents of total flavonoids and hyperoside in FRLE were significantly higher than those in the other polarity extracts. Compared with those in the model group, the levels of triglyceride and total cholesterol decreased, the activities of superoxide dismutase and lactate dehydrogenase increased, and the levels of inflammatory factors interleukin-6 and tumor necrosis factor-α decreased significantly in the cells intervened with FRLE. Moreover, FRLE can regulate lipid metabolism by activating the AMP-activated protein kinase α phosphorylation pathway and increasing the expression of adiponectin. PRACTICAL APPLICATIONS: Fructus rosae laxae (FRL) is an edible medicinal fruit with multiple biological activities, such as antioxidation, anti-inflammatory, and hepatoprotective properties. However, the lipid-lowering activity of FRL and its mechanism of action have not yet been investigated. Our data indicate that the FRL extract, which contains high levels of antioxidant and anti-inflammatory components, plays a beneficial role in regulating lipid metabolism disorders, mainly by regulating the expression of proteins involved in the ADPN/AMPK signaling pathway, and reduces the release of inflammatory factors. Thus, the FRL extract effectively reduces the accumulation of free fatty acids (FFA) in vitro and exhibits considerable potential for the prevention and treatment lipid metabolism disorders.

15.
Hepatology ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068461

RESUMO

BACKGROUND & AIMS: Lysyl oxidase-like 4 (LOXL4) is an amine oxidase that primarily involved in extracellular matrix remodeling and is highly expressed in hepatocellular carcinoma tissues, but its functional role in mediating liver carcinogenesis is poorly understood. Therefore, we aimed to investigate the role of LOXL4 in hepatocarcinogenesis. APPROACH & RESULTS: Here we demonstrate that hepatic LOXL4 expression was increased during the liver carcinogenesis in mice concomitantly fed a choline-deficient, L-amino acid-defined (CDAA) diet. LOXL4 was secreted by the neoplastic cells and primarily localized within hepatic macrophages through exosome internalization. Supplementation of LOXL4 had minimal effect on neoplastic cells. In vitro exposure of macrophages to LOXL4 invoked an immunosuppressive phenotype and activated PD-L1 expression, which further suppressed the function of CD8+ T cells. Injection of LOXL4 promoted macrophages infiltration into the liver and accelerated tumor growth, which was further abolished by adoptive T cell transfer or PD-L1 neutralization. Labelled-free proteomics analysis revealed that the immunosuppressive function of LOXL4 on macrophages primarily relied on IFN-mediated STATs-dependent PD-L1 activation. Hydrogen peroxide scavenge or copper chelation on macrophages abolished the IFN-mediated PD-L1 presentation by LOXL4. In human HCC tissue, expression of LOXL4 in CD68+ cells were positively correlated with that of PD-L1 level. High expression of LOXL4 in CD68+ cells and low expression of CD8A in tumor tissue cooperatively predict poor survival of HCC patients. CONCLUSIONS: LOXL4 facilitates immune evasion by tumor cells and leads to hepatocarcinogenesis. Our study unveils the role of LOXL4 in fostering an immunosuppressive microenvironment during hepatocarcinogenesis.

16.
Curr Med Chem ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33050856

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, with nearly one million new cases and deaths every year. Owing to the complex pathogenesis, hidden early symptoms, rapidly developing processes and poor prognosis, the morbidity and mortality of HCC are increasing yearly. With the progress being made in modern medicine, the treatment of HCC is no longer limited to traditional methods. Targeted therapy and immunotherapy have emerged to treat advanced and metastatic HCC in recent years. Since Sorafenib is the first molecular targeting drug against angiogenesis, targeted drugs for HCC are continually emerging. Moreover, immunotherapy plays a vital role in clinical trials. In particular, the application of immune checkpoint inhibitors, which have received increasing attention in the field of cancer treatment, is a possible research path. Interestingly, these two therapies generally complement each other at some stages of HCC, bringing new hope for patients with advanced HCC. In this paper, we discuss the research progress of targeted therapy and immunotherapy for HCC in recent years, which will provide a reference for the further development of drugs for HCC.

17.
Bioorg Chem ; 105: 104344, 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33091667

RESUMO

PI3Kδ has proved to be an effective target for anti-lymphoma drugs. However, the application of current approved PI3Kδ inhibitors has been greatly limited due to their specific immune-mediated toxicity and increased risk of infection, it is necessary to develop more PI3Kδ inhibitors with new scaffold. In this study, SAR study with respect to piperazinone-containing purine derivatives led to the discovery of a potent and selective PI3Kδ inhibitor, 4-(cyclobutanecarbonyl)-1-((2-(2-ethyl-1H-benzo[d]imidazol-1-yl)-9-methyl-6-morpholino-9H-purin-8-yl)methyl)piperazin-2-one (WNY1613). WNY1613 exhibits good antiproliferative activity against a panel of non-Hodgkin's lymphoma (NHL) cell lines by inducing cancer cell apoptosis and inhibiting the phosphorylation of PI3K and MAPK downstream components. In addition, it can also prevent the tumor growth in both SU-DHL-6 and JEKO-1 xenograft models without observable toxicity. WNY1613 thus could be developed as a promising candidate for the treatment of NHL after subsequent extensive pharmacodynamics and pharmacokinetics investigation.

18.
Adv Ther ; 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33094477

RESUMO

INTRODUCTION: To investigate the risk factors affecting the prognosis of elderly patients with severe aneurysmal subarachnoid hemorrhage (aSAH). METHODS: Forty-nine elderly patients with severe aSAH (Hunt and Hess [H-H] grade III-V) were enrolled in this retrospective study. Follow-up was conducted with patients 30 days after discharge using the Glasgow Outcome Scale (GOS), on which scores of 1-3 indicated a poor outcome (n = 32) and scores of 4-5 indicated a good outcome (n = 17). The patients' general information (gender, age, presence of hypertension, diabetes, or coronary atherosclerotic heart disease, location of ruptured aneurysm, and H-H grade) and complications (cerebral vasospasm, new cerebral infarction, pulmonary infection, liver dysfunction, hypoalbuminemia, anemia, and electrolyte disturbance) were recorded, and comparison between the different outcome groups was undertaken. Univariate analysis was used to analyze the factors associated with different outcomes, and multivariate logistic regression analysis was used to determine the factors that lead to poor outcomes. RESULTS: The incidence of all complications increased in patients with higher H-H grades, but without statistical significance (P > 0.05). There was no statistically significant difference between the two outcome (poor and good) groups in general information and complications (all P > 0.05), with the exception of different H-H grades (P < 0.05). H-H grade was a statistically significant risk factor for poor outcomes in elderly patients with severe aSAH (OR 11.627, 95% CI 2.475-55.556, P = 0.002). CONCLUSION: H-H grade is an independent factor related to the prognosis of elderly patients with severe aSAH.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33073562

RESUMO

Nanostructuring and metal-support interactions have been explored as effective methods to improve the electrocatalytic activity in heterogeneous catalysis. In this study, we have fabricated ultrasmall Ru nanoparticles (NPs) dispersed on S-doped graphene (denoted as Ru/S-rGO) by a facile "one-pot" procedure. The experimental results indicated that both the S doping and moderate degree of oxidization of GO can induce the formation and high dispersion of the ultrasmall Ru NPs with larger electrochemically active surface areas for exposing more active sites. Metal-support interaction between S-doped graphene and Ru NPs was observed from the X-ray photoelectron spectroscopy and electronic charge-difference studies. It resulted in the decrease in the electron density of Ru, which facilitated electron release from H2O and H-OH bond breakage. The results of density functional theory calculation confirmed that the S-dopants could reduce the energy barrier for breaking the H-OH bond to accelerate water dissociation during the alkaline hydrogen evolution reaction (HER). At a current density 20 mA cm-2, the lowest overpotential of 14 mV, superior to that of Pt/C in alkaline solution, was observed for Ru/S-rGO-24. The observed lowest value of overpotential was because of the ultrasmall size, high dispersion, and metal-support interaction. This work provides a simple and effective method in designing advanced electrocatalysts for the HER in an alkaline electrolyte.

20.
Transplant Proc ; 2020 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-32900476

RESUMO

Acute-on-chronic liver failure (ACLF) is a clinical manifestation of acute liver failure and decompensation on the basis of chronic liver disease. To date, hepatitis B virus-related ACLF is still the main cause of liver failure in China. Liver transplantation is currently the most likely treatment option to cure ACLF, but the shortage of donor livers is a barrier to its widespread use. The shortage of organs has led to increased use of expanded-criteria donors (ECDs), that is, donation after cardiac death (DCD) and its variant donation after brain and cardiac death (DBCD-China, DCBD-Switzerland). Here we report a case of liver transplantation, whose recipient was diagnosed with ACLF as a result of use of traditional Chinese medicine while the donor liver was retrieved from a renal transplant patient 4 years after transplantation. This transplant was carried out in accordance with the Helsinki Congress and the Declaration of Istanbul.

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