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1.
FEBS Open Bio ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32027103

RESUMO

Nearly half of human cancers harbor p53 mutations, and mutant p53 (mutp53) promotes carcinogenesis, metastasis, tumor recurrence and chemoresistance. Mutp53 is observed in 30% of breast carcinomas, including triple negative breast cancer (TNBC), and thus mutp53 is a promising target for treatment of TNBC. In this study, we investigated the effect of a PI3K/mTOR dual inhibitor, NVP-BEZ235 (BEZ235), on two TNBC cell lines with mutp53: MDA-MB-231 and MDA-MB-468. Cell growth, migration and colony-formation abilities were detected by MTT, scratch assay, transwell and soft agar assay, revealing that BEZ235 can inhibit the growth, migration and colony-formation abilities of TNBC cells. In addition, BEZ235 caused degradation of mutp53 in these cells. We investigated the underlying mechanism by inhibiting proteasome function using MG132 and inhibiting autophagy using 3-MA and shRNAs. We observed that BEZ235 may induce autophagy through repression of the Akt/mTOR signaling pathway. The observed interplay between mutp53 and autophagy in TNBC cells was examined further by knock down of ATG5 and ATG7, revealing that degradation of mutp53 induced by BEZ235 may be independent of the ubiquitin-proteasome pathway and autophagy mediated by ATG5 and ATG7. Moreover, we found evidence of positive feedback between mutp53 and autophagy in TNBC cells. In conclusion, BEZ235 may exert anti-tumor effects against TNBC cells by targeting mutp53, and this may have implications for the development of future therapies.

2.
Environ Pollut ; 260: 114105, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32041085

RESUMO

Pesticides scarcely exist as individual compounds in the water ecosystem, but rather as mixtures of multiple chemicals at relatively low concentrations. In this study, we aimed to explore the mixture toxic effects of fludioxonil (FLU) and triadimefon (TRI) on zebrafish (Danio rerio) by employing different toxicological endpoints. Results revealed that the 96-h LC50 values of FLU to D. rerio at multiple developmental stages ranged from 0.055 (0.039-0.086) to 0.61 (0.33-0.83) mg L-1, which were less than those of TRI ranging from 3.08 (1.84-5.96) to 9.75 (5.99-14.78) mg L-1. Mixtures of FLU and TRI exerted synergistic effects on embryonic zebrafish. Activities of total superoxide dismutase (T-SOD) and catalase (CAT) were markedly altered in most of the individual and pesticide mixture treatments compared with the control. The expressions of 16 genes involved in oxidative stress, cellular apoptosis, immune system and endocrine system displayed that embryonic zebrafish were affected by the individual pesticides and their mixtures, and greater variations of four genes (ERɑ, Tnf, IL and bax) were found when exposed to pesticide mixtures compared with their individual compounds. Therefore, more studies on mixture toxicities among different pesticides should be taken as a priority when evaluating their ecological risk.

3.
Mar Pollut Bull ; 151: 110832, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32056625

RESUMO

To better understand the uptake, biotransformation and physiological response to tetrabromobisphenol A (TBBPA) in mangrove plants, a short term 14-day hydroponic assay with two mangrove species, Avicennia marina (A. marina) and Kandelia obovata (K. obovata), was conducted. Results showed that two mangrove species could uptake, translocate and accumulate TBBPA from solution. The hydroxylation and debromination metabolites of TBBPA, including OH-TBBPA, TriBBPA, MonoBBPA, and BPA, were found in both mangroves for the first time. The high-level TBBPA suppressed the growth and increased malondialdehyde (MDA) content of K. obovata, did not pose any negative affect on A. marina. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of K. obovata significantly increased in the 7th day, whereas, SOD and POD activities at high-levels of TBBPA became comparable to the control in the 14th day. Contrastingly, the antioxidant enzymes activities of A. marina were positively stimulated by TBBPA during the 14-day of observation, indicating that A. marina was more tolerant of TBBPA.

4.
Int J Biol Macromol ; 149: 532-540, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32004606

RESUMO

This research displayed the structures and thermomechanical feature of starch-based nanocomposites as induced by interaction between propionylated amylose/amylopectin and nanofiller (organically modified montmorillonite). Propionylated amylose incorporated with nanofiller caused some phase separation within the nanocomposites. By contrast, highly-branched propionylated amylopectin favored nanofiller dispersion and disrupted its crystalline structure, and further facilitated certain exfoliated or intercalated structures. Based on these structures, propionylated amylose-rich nanocomposites showed enhanced ß-relaxation in the induced "plasticizer-rich" regions, whereas the propionylated amylopectin nanocomposites displayed higher glass-transition temperature due to restricted macromolecular mobility. These results suggested that the structures and further packaging properties of starch-based nanocomposites could be better understood by controlling the interaction of starch with other ingredients.

5.
Nanotechnology ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32018237

RESUMO

Previous studies have mainly focused on the resistive switching (RS) of amorphous or polycrystalline HfO2-RRAM. The RS of single crystalline HfO2 films has been rarely reported. Yttrium doped HfO2 (YDH) thin films were fabricated and successful Y incorporation into HfO2 was confirmed by X-ray photoemission spectroscopy (XPS). A pure cubic phase of YDH and an abrupt YDH/Si interface were obtained and verified by X-ray diffraction (XRD), Raman spectroscopy and transmission electron microscopy (TEM). A Pt/YDH/n++-Si heterostructure using Si as the bottom electrode was fabricated, which shows stable RS with an ON/OFF ratio of 100 and a reliable data retention (104 s). The electron transport mechanism was investigated in detail. It indicates that hopping conduction is dominating when the device is at a high resistance state (HRS), while space charge limited conduction (SCLC) acts as the dominant factor at a low resistance state (LRS). Such behavior, which is different from devices using TiN or Ti as electrodes, was attributed to the Y doping and specific YDH/Si interface. Our results demonstrate a proof of concept study to use highly doped Si as bottom electrodes along with single crystalline YDH as insulator layer for such RRAM applications as wireless sensors and synaptic simulation.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32043352

RESUMO

The potential in synaptic simulation for neuromorphic computation has revived the research interest of resistive random access memory (RRAM). However, novel applications require reliable multi-level resistive switching (RS), which still represents a challenge. We demonstrate in this work the achievement of reliable HfO2-based RRAM devices for synaptic simulation by performing the Al doping and the post-deposition annealing (PDA). Transmission electron microscopy and operando hard X-ray photoelectron spectroscopy results reveal the positive impact of Al doping on the formation of oxygen vacancies. Detailed I-V characterizations demonstrate that the 16.5% Al doping concentration leads to better RS properties of the device. In comparison with the other reported results based on HfO2 RRAM, our devices with 16.5% Al-doping and PDA at 450°C show better reliable multi-level RS (~20 levels) performance and an increased On/Off ratio. The 16.5%Al:HfO2 sample with PDA at 450°C show good potentiation/ depression characteristics with low pulse width (10 s) along with good On/Off ratio (>1000), good data retention at room temperature and high temperature and good program/erase endurance characteristics with a pulse width of 50 ns. The synapse features including potentiation, depression and spike time dependent plasticity (STDP) were successfully achieved using optimized Al-HfO2 RRAM devices. Our results demonstrate the beneficial effects of Al doping and PDA on the enhancement of the performances of RRAM devices for the synaptic simulation in neuromorphic computing applications.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32034805

RESUMO

The aim of this study was to estimate if methotrexate (MTX) promotes cognitive impairment via increased ER stress and disrupted H2 S signaling in the hippocampus, and if H2 S may alleviate MTX-induced cognitive impairment by inhibiting ER stress through CHOP and caspase-12. Cognitive impairment behaviors were observed by Morris Water Maze test and the apoptosis of neurons were assessed by TUNEL assay. The production of neurons was analyzed by DCX and Ki67 immunohistochemistry. The expressions of CHOP and caspase-12 in the hippocampus were determined by Western blot and immunohistochemistry. MTX increased the expression of CHOP and caspase-12 and the number of TUNEL positive cells in the hippocampus by inhibiting endogenous H2 S-induced neuronal pyknosis in the hippocampal DG region. MTX decreased the number of DCX and Ki67 positive cells in the hippocampus in the hippocampal DG region. The results of Morris water maze showed that MTX could damage the spatial memory of rats. The changes of MTX-induced Morris water maze test in mice and H2 S levels in serum and hippocampus, as well as the expression of CHOP and caspase-12 and the number of CHOP and caspase-12 positive neurons in the hippocampus, indicated that H2 S could alleviate the cognitive impairment induced by methotrexate through CHOP and caspase-12.

8.
Cancer Res ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32015091

RESUMO

Tumor-associated macrophages (TAMs) are important tumor-promoting cells. However, the mechanism underlying how tumor and its microenvironment reprogram these cells remains elusive. Here we report that lipids play a crucial role in TAM generation in tumor microenvironment. Macrophages from both human and murine tumor tissues are enriched with lipids as a result of increased lipid uptake by macrophages. TAMs expressed elevated levels of the scavenger receptor CD36, accumulated lipids, and used fatty acid oxidation (FAO) instead of glycolysis for energy. High levels of FAO promoted mitochondrial oxidative phosphorylation, production of reactive oxygen species, phosphorylation of JAK1 and dephosphorylation of SHP1, leading to STAT6 activation and transcription of genes regulating TAM generation and function. These processes were critical for TAM polarization and activity in vitro and in vivo. In summary, we describe a novel mechanism underlying lipid metabolism-initiated process that promotes the differentiation and function of the protumor TAMs in TME.

9.
Ann Transplant ; 25: e918076, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32015300

RESUMO

BACKGROUND The aim of this study was to investigate the clinical features and treatment strategies of transplant renal artery stenosis (TRAS) with kidneys from donation after cardiac death (DCD). MATERIAL AND METHODS We collected the clinical data of donors and recipients of single-center DCD-induced TRAS from January 2015 to June 2017. RESULTS All the 8 cases of TRAS were from hypertensive cerebrovascular accident DCD-originated kidneys. The mean donor age was 53.5 (45~57) years, with mean BMI 27.8 (26.4~32.3) kg/m², atherosclerosis index 5.8 (4.9~7.0), and renal atherosclerotic plaque. Clinical features of TRAS were: refractory hypertension with elevated serum creatinine >50%, and negative urine protein and occult blood. Ultrasound of transplanted kidneys showed renal blood flow index 0.49 (0.43~0.55). Angiography confirmed the diagnosis of renal artery trunk or secondary branch stenosis. There were 2 cases of moderate stenosis and 6 cases of severe stenosis. Six patients underwent stent implantation and 2 patients underwent balloon dilatation. Seven patients had serum creatinine recovery after interventional therapy during follow-up. The transplanted kidney of 1 patient ruptured 6 h after interventional therapy and was then resected. CONCLUSIONS The incidence of TRAS with hypertensive cerebrovascular accident DCD-originated kidneys is relatively high, which is a warning to kidney transplant physicians. Digital subtraction angiography (DSA) is the most reliable diagnostic means of TRAS and can be performed concurrently with intervention therapy. If the donor has severe atherosclerosis, plaques that are visible to the unaided eye in the renal artery trunk should be removed as completely as possible.

10.
Nat Plants ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32015514

RESUMO

Use of growth retardants enables post-planting optimization of vegetative growth, which is particularly important given ongoing climate change. Mepiquat chloride is an economical and safe retardant widely applied in cotton farming, but it is not uniformly effective. Here, identification of its molecular target as the ent-copalyl diphosphate synthase that initiates gibberellin biosynthesis enabled the introduction of selective agrochemical inhibition, leaving intact more specialized metabolism important for resistance to biotic and abiotic stresses.

11.
Chemistry ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32017296

RESUMO

Capacitive deionization technique is promising in sea water desalination. Compared with common electrode, mixed capacitive deionization electrode exhibits more excellent sea water desalination performance because it integrates pseudocapacitance and electric double layer capacitance in one system. Here, 3D binder free mixed capacitive deionization electrode was fabricated by electro-deposition SiW 12 O 40 4- and polyaniline on 3D exfoliated graphite carrier directly. In this electrode, composite particles with the size about 100 to 120 nm disperse homogenously on 3D exfoliated graphite carrier, which are constructed by SiW 12 O 40 4- and polyaniline. Its specific capacitance achieves 352 F·g -1 at 1 A·g -1 . With current increasing from 1 to 20 A·g -1 , the specific capacitance only decays 32%. When employed in sea water desalination, the performance of this mixed capacitive deionization electrode is also excellent. Under 1.2 V voltage, salt adsorption capacity of this mixed electrode reaches 23.1 mg·g -1 with salt adsorption rate 1.38 mg·g -1 ·min -1 in 500 mg·L -1 NaCl. The performance of this electrode is well retained after 30 cycle test. For this mixed capacitive deionization electrode, its excellent sea water desalination performance originates from the synergistic effect between SiW 12 O 40 4- and polyaniline. This work developed polyoxometalate as a new material for capacitive deionization electrode.

12.
Sci Total Environ ; 711: 134790, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32000324

RESUMO

The growth of global carbon emissions is largely driven by coal-burning in China. China has consumed approximately half of global coal; as such, limiting its coal demand is important for curbing carbon emissions in the country and around the world. Developing effective approaches to limit China's coal demand requires comprehensively understanding the trajectory and drivers shaping its coal footprint in the globalized world. Past studies have investigated production-based coal consumption within China's sovereign territory boundary, but have not addressed consumption-based coal consumption and the globalization background. To fill this research gap, this study conducted a multi-regional input-output analysis - structural decomposition analysis to analyze China's coal footprint in a globalized world. The results show a continued rise in the influence of globalization on China's coal consumption, despite the 1997 Asian financial crisis and 2008-09 global economic crisis lowering of global trade. The percentage of other countries' coal demand on China's production-based coal footprint increased from 20% in 1995 to nearly 30% in 2011, through a global transfer of virtual coal through trade. Virtual coal refers to the coal consumed to support production activities. Meanwhile, the impact of other countries' coal supply on China's consumption-based coal footprint increased from less than 2% in 1995 to more than 4% in 2011. However, the decomposition results showed that domestic demand was the leading contributor to Chinese production-based and consumption-based coal footprint. This was offset by the domestic coal intensity effect. To cut China's coal, strategies to cap its total coal consumption should be considered in a globalized world. Improving energy intensity is an effective approach for the country; China should be leveraging the globalized world, rather than serving as the world's factory through high coal consumption and exports.

13.
Biosci Rep ; 40(2)2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-31922200

RESUMO

Erectile dysfunction (ED) is one of the significant complications of diabetes mellitus (DM), and CASR plays an important role in cellular antiapoptosis and NO production in the vascular endothelium by activating PKC. The present study was aimed to investigate the efficacy of Leech and Centipede Granules (LCG) through the CaSR/PLC/PKC signaling. Fifty male Sprague-Dawley rats were treated with streptozotocin to induce the DM model. After 10 weeks, an apomorphine test was used to confirm DMED. Rats with DMED were administrated with LCG and U73122 for 4 weeks. Fasting blood glucose, body weight, insulin and glucagon levels were measured. Erectile function in rats was assessed by apomorphine. Serums were measured using enzyme-linked immunosorbent assay and flow cytometry, and penile tissues were harvested for histologic and the expression of related targets analyses. After treatment, fasting blood glucose, body weight, insulin, glucagon levels, and erectile function were significantly ameliorated in the LCG groups. The LOX-1, NOX, and EMPs concentrations were significantly decreased with LCG treatment. LCG also continuously increased NO and decreased ET-1 content in penile tissues. LCG and U73122 administration also improved penile fibrosis by significantly decreasing VCAM-1, ICAM-1, and CD62P. The data also showed that LCG reduced the apoptosis level in the penis. Furthermore, the inhibited activation of the CaSR/PLC/PKC pathway was observed in DMED rats with LCG treatment. Collectively, LCG significantly ameliorated erectile function of DMED rats via increased NO generation, inhibiting endothelial cells apoptosis and penile fibrosis, which might benefit from the suppression of CaSR/PLC/PKC pathway in DMED rats.

14.
Mol Carcinog ; 59(3): 304-310, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31912599

RESUMO

Pancreatic carcinoma (PC) is a type of highly lethal malignant tumor that has unfavorable outcomes. One major challenge in improving clinical outcomes is to identify novel biomarkers for prognosis. In this study, we developed an online consensus survival tool for pancreatic adenocarcinoma (OSpaad), which allows researchers and clinicians to analyze the prognostic value of selected genes in PC. OSpaad contains 1319 unique PC cases that have both gene expression data and correspondent clinical data from seven individual cohorts and provides four survival terms including overall survival, disease-specific survival, disease-free interval, progression-free interval for prognosis evaluation. To meet the different research needs, OSpaad allows users to limit survival analysis in subgroups by selecting different terms of clinical confounding factors such as TNM stage, sex, smoking time, lymph invasion, and race. Moreover, we showed that 97% (116 out of 120) previously reported prognostic biomarkers, including ERBB2, TP53, EGFR and so forth, were validated and confirmed their prognostic significance in OSpaad, demonstrating the well performance of survival analysis in OSpaad. OSpaad is a user-friendly online tool with a straightforward interface allowing clinicians and basic research scientists with even a limited bioinformatics background to easily screen and evaluate the prognostic value of genes in a large PC cohort. This online tool can be accessed at http://bioinfo.henu.edu.cn/PAAD/PAADList.jsp.

15.
Toxicon ; 174: 43-47, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31989928

RESUMO

Amanita fuliginea (A. fuliginea) poisoning is an uncommon and potentially fatal amatoxin exposure. We present 3 cases of severe A. fuliginea poisoning associated with thrombocytopenia in China. Three patients consumed foraged A. fuliginea and developed nausea, vomiting, abdominal pain, and diarrhea. They were transferred from primary clinics to our hospital 19-39 h after mushroom ingestion. They all presented with acute hepatic injury, coagulopathy, thrombocytopenia (6-41 × 109/L), and positive fecal occult blood. Intravenous fluids and antioxidants were administered immediately after admission. Fibrinogen and platelets were given to patients A, B and C. Patient A developed fulminant liver failure and died on day 5 after mushroom exposure. Patients B and C recovered and were discharged on days 11 and 9, respectively. The main targets of A. fuliginea poisoning are the liver and digestive tract. To our knowledge this is the first report of thrombocytopenia associated with A. fuliginea ingestion.

16.
Food Chem ; 313: 126163, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31945702

RESUMO

The copigmentation effects of polyphenol with different structures vary greatly. Therefore, the aim of this study is to investigate possible interactions in red wine model solutions between oenin and three phenolic compounds: danshensu, caffeic acid and rosmarinic acid. Our results show that the copigmentation of rosmarinic acid is the strongest among the compounds tested. The colourimetric parameters indicate that colour intensity becomes enhanced with increasing concentration of these copigments, leading to darker and more vivid bluish colours. Thermodynamic and quantum chemical investigations are performed to interpret the absorption properties in the visible range. Fluorescence spectroscopy confirms the interaction between caffeic acid and oenin, while FTIR spectroscopic results further suggest a role for hydrogen bonds in the overall process. To our knowledge, this is the first experimentally corroborated direct evidence of hydrogen bonds in copigmentation.

17.
Cancer Med ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31918459

RESUMO

Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma (NHL) and is a clinical, pathological, and molecular heterogeneous disease with highly variable clinical outcomes. Currently, valid prognostic biomarkers in DLBCL are still lacking. To optimize targeted therapy and improve the prognosis of DLBCL, the performance of proposed biomarkers needs to be evaluated in multiple cohorts, and new biomarkers need to be investigated in large datasets. Here, we developed a consensus Online Survival analysis web server for Diffuse Large B-Cell Lymphoma, abbreviated OSdlbcl, to assess the prognostic value of individual gene. To build OSdlbcl, we collected 1100 samples with gene expression profiles and clinical follow-up information from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. In addition, DNA mutation data were also collected from the TCGA database. Overall survival (OS), progression-free survival (PFS), disease-specific survival (DSS), disease-free interval (DFI), and progression-free interval (PFI) are important endpoints to reflect the survival rate in OSdlbcl. Moreover, clinical features were integrated into OSdlbcl to allow data stratifications according to the user's special needs. By inputting an official gene symbol and selecting desired criteria, the survival analysis results can be graphically presented by the Kaplan-Meier (KM) plot with hazard ratio (HR) and log-rank p value. As a proof-of-concept demonstration, the prognostic value of 23 previously reported survival associated biomarkers, such as transcription factors FOXP1 and BCL2, was evaluated in OSdlbcl and found to be significantly associated with survival as reported (HR = 1.73, P < .01; HR = 1.47, P = .03, respectively). In conclusion, OSdlbcl is a new web server that integrates public gene expression, gene mutation data, and clinical follow-up information to provide prognosis evaluations for biomarker development for DLBCL. The OSdlbcl web server is available at https://bioinfo.henu.edu.cn/DLBCL/DLBCLList.jsp.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31952958

RESUMO

Recently we reported that nucleus accumbens (NAcc) dopamine (DA) tracks uncertainty during operant responding for non-caloric saccharin. We also showed that repeated intermittent exposure to this uncertainty, like exposure to drugs of abuse, leads to sensitization of the locomotor and NAcc DA effects of amphetamine and promotes the subsequent self-administration of the drug. Here we review these findings together with others showing that NAcc glutamate signaling is similarly affected by uncertainty. Extracellular levels of glutamate in this site also track uncertainty in a task in which nose poking for saccharin on an escalating variable ratio schedule of reinforcement is associated with progressively increasing variance between performance of the operant and payout. Furthermore, sensitized behavioral responding to and for amphetamine following exposure to uncertainty is accompanied by increased levels of Ca2+/calmodulin-dependent protein kinase II (CaMKII) and protein kinase C (PKC) phosphorylation as well as altered protein levels of the transcription factor ∆FosB (increased) and glutamate transporter 1 (GLT1; decreased) in NAcc tissues. Notably, phosphorylation by CaMKII and PKC regulates AMPA receptor trafficking and function in this site, is elevated following psychostimulant exposure, and is necessary for the expression of enhanced drug taking. Increased ∆FosB and decreased GLT1 levels are observed following psychostimulant exposure, are associated with increased drug taking and seeking, and are known to modulate AMPA receptors and extracellular glutamate levels respectively. These adaptations in glutamate transmission as well as those observed with DA following repeated intermittent exposure to uncertainty are similar to those produced by exposure to abused drugs. Together, they point to the recruitment of both DA and glutamate signaling pathways in the NAcc in both drug and behavioral addictions. As uncertainty is central to games of chance, these findings have particular relevance for gambling disorders known to exhibit comorbidity with drug abuse.

19.
Ecotoxicol Environ Saf ; 191: 110183, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954220

RESUMO

Toxicokinetics and bioconcentration of eight common bisphenol analogues, including bisphenol A (BPA), -B, -C, -E, -S, -Z, -AF, and -AP in common carp (Cyprinus carpio) were investigated. Both free (BPfree) and total forms (BPtotal) of the bisphenols were measured in various fish tissues. The conjugated forms of bisphenols were calculated based on BPfree and BPtotal. The calculated bioconcentration factors (BCFs) based on the total bisphenols (BPtotal) in the carp whole body were in the range of 0.3-320, agreeing with previous field results from Taihu Lake, China. The elimination rate constant (ke) positively correlated with the fraction of conjugated form (fconjugated), which displayed negative correlation with their log Kow (r = -0.861, p < 0.05), indicating that conjugation facilitated their elimination and those with higher hydrophobicity were more difficult to be eliminated. Except BPA, the concentrations of all bisphenols in the carp tissues were in the order of kidney > liver â‰« muscle. The uptake rate constants (ku) in kidney (r = 0.836, p < 0.05) and in liver (r = 0.863, p < 0.05) displayed significantly positive correlations with BCFs, and ku in kidney was greater than in liver except BPA. These results indicated that kidney and liver played important roles in accumulating bisphenols in carp, and kidney made more contribution than liver for most bisphenols. Biliary excretion predominated for elimination of most bisphenols while BPA and BPS were mainly through urinary excretion.

20.
Aging (Albany NY) ; 12(2): 1186-1200, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31958320

RESUMO

Exposure to particulate matter (PM) is associated with increased incidence of respiratory diseases. The present study aimed to investigate the roles of fibroblast growth factor 10 (FGF10) in PM-induced lung injury. Mice were intratracheally instilled with FGF10 or phosphate-buffered saline at one hour before instillation of PM for two consecutive days. In addition, the anti-inflammatory impact of FGF10 in vitro and its effect on the high-mobility group box 1 (HMGB1)-toll-like receptor 4 (TLR4) pathway was investigated. It was found that PM exposure is associated with increased inflammatory cell infiltration into the lung and increased vascular protein leakage, while FGF10 pretreatment attenuated both of these effects. FGF10 also decreased the PM-induced expression of interleukin (IL)-6, IL-8, tumor necrosis factor-α and HMGB1 in murine bronchoalveolar lavage fluid and in the supernatants of human bronchial epithelial cells exposed to PM. FGF10 exerted anti-inflammatory and cytoprotective effects by inhibiting the HMGB1-TLR4 pathway. These results indicate that FGF10 may have therapeutic values for PM-induced lung injury.

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