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1.
J Colloid Interface Sci ; 630(Pt B): 281-289, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36327731

RESUMO

Layered oxides based on manganese (Mn), rich in lithium (Li), and free of cobalt (Co) are the most promising cathode candidates used for lithium-ion batteries due to their high capacity, high voltage and low cost. These types of material can be written as xLi2MnO3·(1 - x) LiTMO2 (TM = Ni,Mn,etc.). Though, Li2MnO3 is known to have poor cycling stability and low capacity, which hinder its industrial application commercially. In this work, Li1.2Ni0.2Mn0.6O2 materials with different amounts of structural defects was successfully synthesized using powder metallurgy followed by different cooling processes in order to improve its electrochemical properties. Microstructural analyses and electrochemical measurements were carried out on the study samples synthesized by a combination of X-ray diffraction, transmission electron microscopy, and cyclic voltammetry. It is found that the disorder of the transition metal layer in Li2MnO3 promotes its electrochemical activity, whereas the Li/Ni antisites of the Li layer maintain the stability of its local structure. The material with optimal amount of structural defects had an initial capacity of 188.2 mAh g-1, while maintaining an excellent specific capacity of 144.2 mAh g-1 after 500 cycles at 1C. In comparison, Li1.2Ni0.2Mn0.6O2 without structural defect only gives a capacity of 40.8 mAh g-1 after cycling. This microstructural control strategy provides a simple and effective route to develop high-performance Co-free, Li-rich Mn-based cathode materials and scale-up manufacturing.

2.
Neural Regen Res ; 18(3): 603-608, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36018184

RESUMO

ß2-Microglobulin (ß2M), a component of the major histocompatibility complex class I molecule, is associated with aging-related cognitive impairment and Alzheimer's disease. Although upregulation of ß2M is considered to be highly related to ischemic stroke, the specific role and underlying mechanistic action of ß2M are poorly understood. In this study, we established a rat model of focal cerebral ischemia by occlusion of the middle cerebral artery. We found that ß2M levels in the cerebral spinal fluid, serum, and brain tissue were significantly increased in the acute period but gradually decreased during the recovery period. RNA interference was used to inhibit ß2M expression in the acute period of cerebral stroke. Tissue staining with 2,3,5-triphenyltetrazolium chloride and evaluation of cognitive function using the Morris water maze test demonstrated that decreased ß2M expression in the ischemic penumbra reduced infarct volume and alleviated cognitive deficits, respectively. Notably, glial cell, caspase-1 (p20), and Nod-like receptor pyrin domain containing 3 (NLRP3) inflammasome activation as well as production of the inflammatory cytokines interleukin-1ß, interleukin-6, and tumor necrosis factor-α were also effectively inhibited by ß2M silencing. These findings suggest that ß2M participates in brain injury and cognitive impairment in a rat model of ischemic stroke through activation of neuroinflammation associated with the NLRP3 inflammasome.

3.
Front Cardiovasc Med ; 9: 934914, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36324757

RESUMO

Background and aims: Proprotein convertase subtilisin/kexin type 9 (PCSK9) levels could predict cardiovascular event in patients with well-controlled LDL-C levels, suggesting an LDL-independent mechanism of PCSK9 on the cardiovascular system. Accumulating evidence suggests PCSK9 might be associated with increased platelet reactivity. This study aimed to assess the relationship between PCSK9 levels and platelet reactivity in subjects not taking statins or antiplatelet agents. Methods: A cross-sectional study was conducted to investigate the independent contribution of PCSK9 to platelet activity by controlling for the potential confounding factors. The study population included 89 subjects from a health examination centre who underwent routine annual health check-ups or had an examination before a selective operation. Subjects taking statins or antiplatelet agents were excluded. Adenosine diphosphate (ADP)-induced platelet aggregation was determined by PL-11 platelet analyzer using impedance aggregometry and plasma PCSK9 levels were determined using an ELISA. Serum Lipid profile was assessed by measuring the concentration of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG), with low-density lipoprotein cholesterol (LDL-C) being directly measured using enzymatic techniques. The association between PCSK9 and platelet reactivity was investigated. Results: The study subjects were composed of 53 males and 36 females with an average age of 55 (±11) years old. The univariate correlation analysis showed significant correlation between ADP-induced maximal aggregation rate (MAR) and PCSK9 (r = 0.55, p < 0.001) as well as TC (r = 0.23, p = 0.028), LDL-C (r = 0.27, p < 0.001), and PLT (r = 0.31, p = 0.005). Being male (41.2% vs. 46.6, p = 0.04) and smoking (37.4 vs. 46.2%, p = 0.016) were associated with lower ADP-induced MAR than being female and non-smoking. However, there is no correlation between PCSK9 and AA-induced platelet maximal aggregation rate (r = 0.17, p = 0.12). Multiple regression analysis suggested that PCSK9 contributed independently to ADP-induced maximal aggregation rate (ß = 0.08, p = 0.004) after controlling for the effect of TC, LDL-C, PLT, being male, and smoking. Conclusions: PCSK9 is positively associated with platelet reactivity, which may partly account for the beneficial effect of PCSK9 inhibition in reducing the risk of major adverse cardiovascular events after acute coronary syndrome (ACS).

4.
Drug Des Devel Ther ; 16: 3805-3816, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36349306

RESUMO

Purpose: The prevalence of hyperlipidemia and related illnesses is on its rise, and atorvastatin is the frequently used hypolipidemic agent. However, there is still uncertainty about the mechanisms, especially the relationship between the lipid-lowering effect, intestinal microbiome, and metabolic profiles. We aim to intensively explain the mechanism of the hypolipidemic effect of atorvastatin through multi-omics perspective of intestinal microbiome and metabolomics. Methods: Multi-omics methods play an increasingly important role in the analysis of intestinal triggers and evaluation of metabolic disorders such as obesity, hyperlipidemia, and diabetes. Therefore, we were prompted to explore intestinal triggers, underlying biomarkers, and potential intervention targets of atorvastatin in the treatment of dyslipidemia through multi-omics. To achieve this, SPF Wistar rats were fed a high-fat diet or normal diet for 8 weeks. Atorvastatin was then administered to high-fat diet-fed rats. Results: By altering intestinal microbiome, a high-fat diet can affect feces and plasma metabolic profiles. Treatment with atorvastatin possibly increases the abundance of Bacteroides, thereby improving "propanoate metabolism" and "glycine, serine and threonine metabolism" in feces and plasma, and contributing to blood lipid reduction. Conclusion: Our study elucidated the intestinal triggers and metabolites of high-fat diet-induced dyslipidemia from the perspective of intestinal microbiome and metabolomics. It equally identified potential intervention targets of atorvastatin. This further explains the mechanism of the hypolipidemic effect of atorvastatin from a multi-omics perspective.


Assuntos
Dieta Hiperlipídica , Hiperlipidemias , Animais , Ratos , Dieta Hiperlipídica/efeitos adversos , Atorvastatina/farmacologia , Bacteroides , Metabolismo dos Lipídeos , Ratos Wistar , Hiperlipidemias/tratamento farmacológico , Lipídeos
5.
Artigo em Inglês | MEDLINE | ID: mdl-36348237

RESUMO

Water resources, as one of the indispensable resources for urban development, have become an important factor limiting the sustainable development of cities. In order to promote sustainable urban development, Beijing has set the work task of reaching 99% of urban and rural wastewater treatment rate from 2020 to 2035. Accurate prediction of future wastewater discharge is essential to achieve the target. For this reason, this study takes Beijing as the research object and constructs a combined prediction model based on gray relational analysis and long- and short-term memory (GRA-LSTM). Firstly, gray relational analysis (GRA) is used to analyze the correlation of the experimental data indicators affecting the amount of wastewater discharged in order to obtain experimental data indicators with stronger correlation. Secondly, the long short-term memory (LSTM) model was used to learn the characteristics of the key impact indicators and obtain the optimal model parameters. The results showed that the mean absolute percentage error (MAPE) value of the combined GRA-LSTM model constructed in this study was 5.62%, and the prediction accuracy was higher than that of the other seven prediction models. Then, three scenarios with low, medium, and high dimensions were set to predict the wastewater discharge in Beijing from 2020 to 2035, and the prediction result that the wastewater discharge in Beijing will still continue to grow was obtained. Finally, in order to improve the water utilization rate and promote the sustainable development of the city, this study proposes relevant policy recommendations in terms of the unbalanced urban-rural development of Beijing's wastewater treatment capacity and the increase of recycled water usage.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36397266

RESUMO

OBJECTIVE: It remains controversial whether primary tumor resection (PTR) improves survival in patients with asymptomatic, unresectable metastatic colorectal cancer (mCRC). Therefore, we conducted a meta-analysis to assess the latest evidence on clinical outcomes. MATERIALS AND METHODS: We systematically searched PubMed, Web of Science, Cochrane Library, and Embase databases for eligible studies published between database inception and May 2022. RevMan 5.4 and Stata 16.0 were used for the meta-analysis. RESULTS: A total of nine studies were included, including four randomized controlled trials (RCTs) and five retrospective cohort studies. Meta-analysis showed that overall survival (OS) [HR = 0.89, 95%CI (0.74, 1.06), P = 0.19] and progression-free survival (PFS) [HR = 0.87, 95%CI (0.71, 1.06), P = 0.17] were not significantly different between the PTR and non-PTR groups. In the subgroup analysis, all subgroups showed no significant difference in OS between the two groups. CONCLUSION: PTR may not provide additional survival benefits over chemotherapy in asymptomatic, unresectable mCRC patients. However, in view of the limitations of this study, more well-designed RCTs are needed to validate our conclusions.

7.
Eur J Pharmacol ; : 175391, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36400161

RESUMO

Epilepsy is a chronic disease that affects a wide range of people. Furthermore, a third of patients suffering from epileptic seizures do not respond to antiepileptic drugs. In recent years, increasing attention has focused on the role of oxidative stress in acquired epilepsy, and adjuvant antiepileptic drugs to reduce oxidative stress may be a new therapeutic strategy. In this study ginsenoside Rh2 was resistant to oxidative stress induced by epileptic activity in vivo and in vitro. Using online databases, we identified forkhead box O3a (FOXO3a) overexpression in epilepsy tissue and validated this in vitro, in vivo, and in clinical tissues of patients with epilepsy. An in vitro epilepsy model revealed that the overexpression of FOXO3a led to more severe oxidative stress, while the knockdown of FOXO3a had a protective effect on SH-SY5Y cells. Moreover, our results showed that the positive effect of FOXO3a on oxidative stress was caused by the transcriptional activation of Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of nuclear factor erythroid 2-related factor 2 (NRF2). We also found that ginsenoside Rh2 can directly inhibit the activation of FOXO3a by selectively blocking CREB-binding protein (CBP)/p300-mediated FOXO3a acetylation and play a role in regulating the KEAP1-NRF2 pathway to resist oxidative stress.

8.
Clin Immunol ; 245: 109176, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36368640

RESUMO

Atherosclerosis, characterized as the chronic inflammation of the arterial wall, is one of the leading causes of coronary artery disease (CAD), and macrophages are found to play essential roles in the initiation and progression of inflammation in atherosclerosis. N6-methyladenosine (m6A) modification, as the most abundant epi-transcriptomic modification in mRNA, is found to mediate the atherogenic inflammatory cascades in vascular endothelium. The detailed molecular mechanism of m6A methylation regulating inflammatory response during atherosclerosis is still not fully known. In this study, we find oxidized low-density lipoprotein (oxLDL) stimulation increases methyltransferases Mettl3 and Mettl14 expressions in macrophages, whereas the total m6A modification level in macrophages decreases under oxLDL stimulation. Matrin-3 (Matr3), an RNA binding protein, is identified to play a suppressive role on oxLDL-mediated macrophage inflammatory responses through inhibiting activation of pro-inflammatory signaling, mitogen-activated protein kinase (Mapk) by m6A-mediated mRNA decay via regulating the formation of Mettl3-Mettl14 complex. Moreover, we find that Matr3 expression decreases in the oxLDL-stimulated macrophages, and the peripheral blood-derived monocytes from patients with CAD, and overexpression of Matr3 significantly alleviates atherosclerosis development in vivo. Our study for the first time clarifies the role of Matr3 on macrophage inflammatory responses during atherosclerotic development, and supplies deep understanding on the relationship of m6A modification and inflammatory responses in atherosclerosis.

9.
Sci Total Environ ; 858(Pt 2): 159831, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36336049

RESUMO

Continuous measurements of volatile organic compounds (VOCs), ozone (O3), fine particulate matter (PM2.5), and related parameters were conducted between April 2020 and March 2021 in Beijing, China, to characterize potential sources of VOCs and their impacts on secondary organic aerosols (SOAs) and O3 levels. The annual average mixing ratio of VOCs was 17.4 ± 10.1 ppbv, with monthly averages ranging from 11.6 to 25.2 ppbv. According to the empirical kinetic modeling approach (EKMA), O3 formation during O3 season was "VOCs-limited", while it was in a "transition" regime during O3 pollution episodes. In the O3 season, higher ozone formation potential (OFP) of m/p-xylene, o-xylene, toluene, isopentane, and n-butane were evident during O3 pollution episodes, in line with the increasing contributions of solvent usage and coating, as well as gasoline evaporation to OFP obtained through a matrix factorization model (PMF). Aromatics contributed the most to the secondary organic aerosol formation potential (SOAFP). In the non-O3 season, the contribution of vehicle exhaust to SOAFP elevated on hazy days, thereby revealing the importance of traffic-derived VOCs for PM2.5 pollution. Our results indicate that the prior control of different VOC sources should vary by season, thereby facilitating the synergistic control of O3 and PM2.5 in Beijing.

10.
J Environ Manage ; 326(Pt A): 116676, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36368205

RESUMO

Sintering dust from the steelmaking industry is a hazardous waste that is rich in valuable metals. The purpose with the present study has been to design an efficient process for the preparation of K2SO4 and NH4Cl fertilizers by using sintering dust as raw material. The K, S, and Cl in the sintering dust were selectively and efficiently leached using water. The leaching of Ca impurities was then greatly reduced and the appearance of Zn and Mg was avoided. The Cl- ions in the leachate were, thereafter, adsorbed by a 201 × 7 resin to form a K2SO4 solution. Finally, the loaded Cl- on the resin was desorbed to form a NH4Cl solution, and the resin was regenerated and recycled. The purified solutions were crystallized to prepare K2SO4(s) and NH4Cl(s) products, which met the national standard of China for superior potassium sulfate and ammonium chloride, to be used for agricultural use. The recoveries of K, Cl, and S from the sintering dust were 80.78%, 92.63%, and 93.92%, respectively. Notably, the Mn content in the leaching residue increased from 9.08% to 14.19%. This could be used for the conversion of Mn impurities into recyclable manganese-rich raw materials. This green process enables an effective extraction of important impurities in hazardous sintering dust, thereby providing a new potassium source for potash fertilizer manufacturing with notable economic and environmental benefits.

11.
Sci Rep ; 12(1): 19671, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36385511

RESUMO

Cloud and aerosol are two important modulators that influence the solar radiation reaching the earth's surface. It is intriguing to find diverse impacts of clouds and aerosols over Southern China (SC) and Northern India (NI) which result in remarkable differences in the plane-of-array irradiance (POAI) that signifies the maximum available solar photovoltaic potential by combining the latest satellite retrieval results and modeling tools. By separating the impacts of cloud and aerosol on the POAI, it is found that clouds are responsible for the most reduction of POAI in the SC, while aerosols and clouds are equally important for the NI region. The frequent occurrences of low and middle level clouds with high optical depth in the SC, as compared with the much lower occurrences of all levels of clouds with lower optical depth in the NI, is regarded as the major reason for the differences in the POAI. The differences in the main compositions of aerosols in the SC (sulfate) and the NI (dust) could be essential to answer the question of why higher aerosol optical depth in the SC whereas leads to weaker reduction in the POAI than that in the NI. The mitigation measures targeting on the controls of different types of aerosols should be considered for different regions.

12.
Acta Biomater ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36402295

RESUMO

Ureteral stricture disease (USD) is a common urologic condition. Patients with ureteral stricture disease may suffer from ipsilateral flank pain, nausea, urinary calculi, infection, and impaired renal function. The treatments of USD include surgery, followed by implantation of the ureteral stent to aid the drainage of the urine. The traditional ureteral stent may sometimes cause urological infection, encrustation, and discomfort. To decrease the complication of the ureteral stent, we modified the structure and material based on the traditional ureteral stent. The traditional nondegradable Double-J shape tubular ureteral stent was turned into the biodegradable mesh ureteral stent. The modified mesh ureteral stent and Double-J ureteral stent were inserted into the ureters of the USD animals, respectively. The results of the gross morphology, serology, urinalysis, histology, microstructure, et al. demonstrated that modified mesh ureteral stent has a favorable ability in supporting the ureter and has no effect on cell proliferation, migration, apoptosis, and cell cycle of the human uroepithelial cells. The mesh ureteral stent could relieve ureter obstruction and can be slowly biodegraded after 3-5 months of implantation without the need for a second surgery to remove the stent. Compared to the Double-J ureteral stent, the modified mesh ureteral stent has a lower rate of urinary tract infection and less encrustation. It is expected to be an alternative treatment approach for USD. However, due to the limited number of animals and clinical data, further study focused on the application value in clinical practice are essential. STATEMENT OF SIGNIFICANCE: This study demonstrates: 1. A modified biodegradable mesh ureteral stent; 2. Without the need for a second surgery to remove the stent; 3. A lower rate of urinary tract infection and less encrustation than a double-J ureteral stent; 4. An alternative treatment approach for USD.

13.
J Burn Care Res ; 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36402740

RESUMO

This work aims to elucidate the molecular mechanism of Qi Wei anti-burn Tincture (QW) on wound healing in burnt mice using metabolomics and molecular biology techniques. A scald model was first established in Kunming mice. After treatment, biochemical indicators for liver function and burnt skin tissues were then evaluated via biochemical detection and HE staining respectively. Liver tissues were further analyzed for differential metabolites, inflammatory factors, and mRNA levels of cytokines using metabolomics and molecular biology techniques. Involved metabolic pathways were also identified using software. Qi Wei anti-burn Tincture treatment did promote the healing of the burn wounds on Kunming mice with a downregulation of ALP, ALT, AST to normal levels. In mouse liver tissue, the contents of glutamine, aspartic acid, succinic acid and citrulline were significantly reduced, while the contents of 5-hydroxyproline, taurine, hypotaurine and glutamic acid significantly increased. These major differential compounds are involved in the arginine metabolic pathway, nitrogen excretion, and the metabolism of taurine and hypotaurine, suggesting that Qi Wei anti-burn Tincture reprogramed the above metabolic processes in the liver. Furthermore, the application of Qi Wei anti-burn Tincture increased the expression of TGF-ß1 and FGF-2, and reduced the levels of TNF-α, IL-1ß, IL-6 and reactive oxygen species in the liver of mice induced by burn injury. This study found that Qi Wei anti-burn Tincture treatment promoted metabolic pathway remodeling in liver, which might be a potential mechanism for Qi Wei anti-burn Tincture to treat burn wounds.

14.
J Environ Manage ; 326(Pt A): 116644, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36370611

RESUMO

Humans and water are closely connected in large river basins and form social-ecological systems (SESs). However, cross-scale effect in SESs make it difficult to identify the key forces driving human-water connections at the community scale when ignoring the landscape context. Focusing on the incongruous human-water relationships in the lower reaches of the Yellow River, we built local resident perception-based networks linking the agricultural subsystem, environmental subsystem, and cultural subsystem by distributing farmer household questionnaires and extracted 13 indicators from 7 kinds of network metrics to indicate human-water connections. We applied analysis of variance (ANOVA), random forest (RF) and multilevel linear model (MLM) methods to identify the driving forces of perception-based human-water connections among 20 factors at both the community and landscape scales. The results showed that the perception-based network indicators were mainly directly influenced by community-level driving factors, especially the accessibility of information, such as the frequency of going out, the frequency of accessing the Yellow River channel, and the information source for the national policy on the Yellow River. The influences of community-level driving factors on network indicators were affected by landscape-level driving factors, e.g., the nighttime light, population density, gross domestic product and proportion of artificial land, thus indicating indirect influences from the landscape context. These analyses and findings can enrich the methods by which social, ecological and hydrological elements are structurally linked in sociohydrologic research and highlight the cross-scale effect of the landscape context on human-water systems at the community level.

15.
Dev Comp Immunol ; : 104589, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36403789

RESUMO

In mammals, type II interferon (IFN; i.e. IFN-γ) signalling transduces through its specific receptors IFN-γR1 and IFN-γR2. In an osteoglossiform fish, the arapaima Arapaima gigas, three type II IFNs, IFN-γ-like, IFN-γ and IFN-γrel, and their four possible receptor subunits IFN-γR1-1, IFN-γR1-2, IFN-γR2-1 and IFN-γR2-2 were identified in this study. The three type II IFN genes are composed of four exons and three introns, and they all contain IFN-γ signature motif and signal peptide, with the presence of potential nuclear localization signal (NLS) in IFN-γ-like and IFN-γ. The IFN-γR1-1, IFN-γR1-2, IFN-γR2-1 and IFN-γR2-2 are composed of seven exons and six introns, with predicted IFN-γR1-1 and IFN-γR1-2 proteins containing JAK1 and STAT1 binding sites, and IFN-γR2-1 and IFN-γR2-2 containing JAK2 binding sites. Gene synteny analysis showed that the type II IFN and their receptor loci are duplicated in arapaima. All these genes were expressed constitutively in all organs/tissues examined, and responded to the stimulation of polyI:C. The prokaryotic recombinant IFN-γ-like, IFN-γ and IFN-γrel proteins can significantly induce the upregulation of immune-related genes in trunk kidney leucocytes. The ligand-receptor relationship analyses revealed that recombinant IFN-γ-like, IFN-γ, and IFN-γrel transduce downstream signalling through IFN-γR1-1/IFN-γR2-1, IFN-γR1-2/IFN-γR2-2, and IFN-γR1-1, respectively, in xenogeneic cells with the overexpression of original or chimeric receptors. In addition, tyrosine (Y) 366 and Y377 in the intracellular region may be essential for the function of IFN-γR1-2 and IFN-γR1-1, respectively. The finding of type II IFN system in A. gigas thus provides different knowledge in understanding the diversity and evolution of type II IFN ligand-receptor relationships in vertebrates.

16.
Bioorg Chem ; 130: 106220, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36347088

RESUMO

Photothermal therapy (PTT) has gained extensive interest in tumor treatments due to its non-invasive and low-toxic nature. However, the currently available photothermal agents (PTAs) mostly show unsatisfactory photothermal conversion efficiency (PCE). Besides, as a local cancer treatment modality, PTT fails to inhibit metastasis of tumors. To address these issues, in this study, two aza-boron-dipyrromethene (aza-BODIPY)-based organic photothermal agents (OPTAs), Fc-aza-BODIPY and TPA-aza-BODIPY, were rationally coined by introducing two strong electron-donating ferrocene (Fc) moieties and two triphenylamine (TPA) rotors, which could boost intramolecular photo-induced electron transfer (PET) and molecular rotation respectively, thereby improving the PCE of aza-BODIPY dyes. After encapsulation of hydrophobic Fc-aza-BODIPY (or TPA-aza-BODIPY) and quercetin with biodegradable PLGA and DSPE-mPEG2000, the resulting nanoparticles (FAQ NPs and TAQ NPs) showed excellent optical properties with PCE of ∼72.0% and ∼79.7% and specific tumor accumulations through enhanced permeability and retention (EPR) effects. Consequently, these two NPs possessed prominent antitumor effects under 880 nm laser irradiation. Moreover, both FAQ NPs and TAQ NPs loaded with quercetin could inhibit tumor metastasis efficiently. These two multifunctional nanomaterials integrating OPTAs and anti-metastasis agents constructed a cooperative treatment program, which may provide a potential opportunity for future clinical cancer treatment.

18.
Front Psychol ; 13: 1029081, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36337470

RESUMO

Previous studies have paid more attention to the impact of non-balanced reciprocity in the organization on employees' behaviors and outcomes, and have expected that the reciprocity norm could improve employees' compliance behavior. However, there are two distinct types of non-balanced reciprocity, and whether generalized reciprocity affects employees' compliance behavior rather than negative reciprocity and its mechanisms has not been further explored so far. Building on the social exchange theory and cognitive appraisal theory, we established and examined a model in a scenario-based experiment across a two-stage survey of 316 participants. In this article, we propose that generalized reciprocity (relative to negative reciprocity) positively influences employees' compliance behavior, and thriving at work mediates its relationship. Furthermore, we argue that the positive association between generalized reciprocity and thriving at work is moderated by the perceived cognitive capabilities of artificial intelligence (AI). This association is amplified for people high in the perceived cognitive capabilities of AI. We also propose that the positive association between thriving at work and compliance behavior is moderated by conscientiousness, such that the association is amplified for people high in conscientiousness. These findings have theoretical and practical implications.

19.
Front Genet ; 13: 990594, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339006

RESUMO

5-Methyladenosine (m5C) is a type of epigenetic modification involved in the progression of various cancers. To investigate the role of m5C-related long non-coding RNAs (lncRNAs) in the prognosis and immune cell infiltration in hepatocellular carcinoma (HCC), we obtained patients' clinical information and transcriptome data of HCC from the Cancer Genome Atlas (TCGA) database. We applied Pearson correlation analysis to construct an m5C-related lncRNA-messenger RNA (mRNA) co-expression network. Univariate Cox analysis, least absolute shrinkage and selection operator (LASSO), and multivariate Cox analysis were employed to establish an m5C-related lncRNA prognostic risk model. We then verified the model using Kaplan-Meier analysis, principal component analysis, as well as univariate and multivariate Cox analyses. The expression of m5C-related lncRNAs was validated in HCC tissues and different cell lines. Combining the risk score and clinicopathological features, a nomogram was established for predicting the overall survival (OS) of HCC patients. Furthermore, gene set enrichment analysis (GSEA) revealed that some tumor-associated pathways were significantly enriched in the high-risk group. Immune cell infiltration analysis demonstrated that the levels of Treg cells, neutrophils, and M2 macrophages were higher in the high-risk group. In addition, patients with high tumor mutation burden (TMB) had worse OS than those with low TMB. We also assessed the immune checkpoint level and chemotherapeutic agent sensibility. Then in vitro experiments were performed to examine the biological function of MKLN1-AS in HCC cells and found that knockdown of MKLN1-AS suppressed the proliferation, migration, and invasion. In conclusion, m5C-related lncRNAs played a critical role in predicting the prognosis of patients with HCC and may serve as new therapeutic targets for HCC patients.

20.
Pathol Oncol Res ; 28: 1610538, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36405393

RESUMO

Background: The study aimed to detect DEGs associated with BRCA bone metastasis, filter prognosis biomarkers, and explore possible pathways. Methods: GSE175692 dataset was used to detect DEGs between BRCA bone metastatic cases and non-bone metastatic cases, followed by the construction of a PPI network among DEGs. The main module among the PPI network was then determined and pathway analysis on genes within the module was performed. Through performing Cox regression, Kaplan-Meier, nomogram, and ROC curve analyses using GSE175692 and GSE124647 datasets at the same time, the most significant prognostic biomarker was gradually filtered. Finally, important pathways associated with prognostic biomarkers were explored by GSEA analysis. Results: The 74 DEGs were detected between bone metastasis and non-bone metastasis groups. A total of 15 nodes were included in the main module among the whole PPI network and they mainly correlated with the IL-17 signaling pathway. We then performed Cox analysis on 15 genes using two datasets and only enrolled the genes with p < 0.05 in Cox analysis into the further analyses. Kaplan-Meier analyses using two datasets showed that the common biomarker AGR2 expression was related to the survival time of BRCA metastatic cases. Further, the nomogram determined the greatest contribution of AGR2 on the survival probability and the ROC curve revealed its optimal prognostic performance. More importantly, high expression of AGR2 prolonged the survival time of BRCA bone metastatic patients. These results all suggested the importance of AGR2 in metastatic BRCA. Finally, we performed the GSEA analysis and found that AGR2 was negatively related to IL-17 and NF-kß signaling pathways. Conclusion: AGR2 was finally determined as the most important prognostic biomarker in BRCA bone metastasis, and it may play a vital role in cancer progression by regulating IL-17 and NF-kB signaling pathways.


Assuntos
Neoplasias Ósseas , Neoplasias da Mama , Humanos , Feminino , Neoplasias da Mama/patologia , Prognóstico , Interleucina-17 , Neoplasias Ósseas/genética , Estimativa de Kaplan-Meier , Mucoproteínas , Proteínas Oncogênicas
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