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1.
J Cell Physiol ; 236(2): 1418-1431, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32677057

RESUMO

Esophageal squamous cell carcinoma (ESCC) is one of the deadliest cancers, and long noncoding RNAs (lncRNAs) regulate gene expression or activities. This study investigated the role of lncRNA LINC00551 in ESCC development and progression. Three paired ESCC and normal tissues were subjected to next-generation sequencing and we identified 82 upregulated and 60 downregulated lncRNAs, including LINC00551, which was confirmed to markedly downregulated in 78 ESCC tissues and in the Gene Expression Profiling Interactive Analysis data set. Downregulated LINC00551 expression was associated with lymph node metastasis, advanced TNM stage, and tumor size. Moreover, downregulated LINC00551 expression was also associated with poor progression-free survival and overall survival of ESCC patients. In vitro and in vivo, LINC00551 overexpression inhibited ESCC cell proliferation and invasion, whereas knockdown of LINC00551 expression promoted ESCC cell proliferation and invasion. RNA pull-down and mass spectrometry assays identified the potential LINC00551 binding proteins, and HSP27 was a promising LINC00551 targeting proteins after RNA immunoprecipitation assay. At the protein level, LINC00551 bound to and decreased HSP27 phosphorylation, and in turn, downregulated ESCC cell proliferation and invasion. The current study demonstrated the functional significance of LINC00551 in ESCC development, progression, and prognosis. Further study will assess LINC00551 as a novel prognostic marker or therapeutic target for ESCC.

2.
Sci Total Environ ; 750: 141717, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32862005

RESUMO

Erosion-induced soil carbon loss has been identified as a critical process in the global carbon (C) cycle. Surface coverage substantially alters the soil erosion process and the effects of net loss or deposition on soil organic C (SOC). However, information on SOC loss induced by soil erosion at the process level is limited. The aim of this study was to investigate how runoff and erosion regimes affect dissolved and sediment-bound organic C (DOC and SBOC) loss. Thus, six simulated rainfall events were conducted on two laboratory plots (9.75 m × 1.83 m) with different surface coverages (17-83%) and coverage distributions (upslope vs. downslope) using polypropylene geotextiles. The results showed that the variability in the process of runoff and sediment yield existed as a result of altered surface coverage over the erosion zone (SSerosion zone) and covered zone (SScovered zone) on the slope. Thus, the erosion regimes can be identified as deposition- and transport-dominated processes, which were the main soil erosion subprocesses. The surface coverage located downslope (SCtop-bottom slope) can more efficiently reduce runoff (21.9-85.7%) and sediment (67.6-98.3%) than the SCbottom-top slope (runoff: 20.1-83.0%; sediment: 35.0-93.3%), which has the surface coverage located upslope. DOC (8.0-11.3 mg L-1) and SBOC (0.3-0.5 mg g-1) in the deposition-dominated process on the SCtop-bottom slope were higher than in the transport-dominated process on the SCbottom-top slope (DOC: 6.8-10.2 mg L-1; SBOC: 0.2-0.3 mg g-1). The loading of DOC and SBOC was largely dependent on runoff and sediment yield, and DOC load contributed 83.9-89.7% of the SOC loss. Overall, laboratory results highlighted the soil C loss at different hydrological and erosion regimes (deposition- vs. transport-dominated process). This study provides important information that can be used to facilitate further implementations such as watershed modeling of soil C dynamics and the corresponding decision-making processes.

3.
Talanta ; 222: 121544, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33167251

RESUMO

96-Well technology is associated with automated sample preparation and simultaneous analysis based on the low-cost well plate format. To explore the potential applications of 96-well technology in SERS detection, we examined the surface-bound electroless deposition procedure for the preparation of uniform and stable Ag mirror films on polydopamine (PDA)-coated well plates as active-SERS substrates. In the presented procedure, small Ag seeds assembled on PDA coating were employed as the surface-bound catalyst and provided the active sites for electroless Ag deposition. The high-quality Ag mirror films showed high performance in terms of sensitivity, uniformity, reproducibility and stability using rhodamine 6G (R6G) as the probe molecule. A remarkable enhancement factor of 3.41 × 108 was obtained. The relative standard deviations against well-by-well and batch-by-batch reproducibility were less than 5%. The SERS films on well plates were successfully used to quantify the amounts of organic dyes (R6G and malachite green) in environmental water samples and small biological molecules (adenosine triphosphate and adenine) in urine matrix, displaying satisfactory sensitivity, selectivity and recovery. Their limit of detection values were at nanomolar, even picomolar concentration.

4.
Bioresour Technol ; 320(Pt B): 124391, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-33220546

RESUMO

Molybdenum disulfide nanoparticles (MoS2 NPs) hold tremendous properties in wide domain of applications. In this study, the impact of MoS2 NPs was investigated on algal physiological and metabolic properties and a two-stage strategy was acquired to enhance the commercial potential of Dunaliella salina. With 50 µg/L of MoS2 NPs exposure, cellular growth and biomass production were promoted by 1.47- and 1.33-fold than that in control, respectively. MoS2 NPs treated cells were subject to high light intensity for 7 days after 30 days of normal light cultivation, which showed that high light intensity gradually increased ß-carotene content by 1.48-fold. Furthermore, analyses of primary metabolites showed that combinatorial approach significantly altered the biochemical composition of D. salina. Together, these findings demonstrated that MoS2 NPs at an optimum concentration combined with high light intensity could be a promising approach to concurrently enhance biomass and ß-carotene production in microalgae.

5.
BMC Psychiatry ; 20(1): 529, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33167901

RESUMO

BACKGROUND: Anhedonia is a core clinical symptom of mental disorders. The Revised Physical Anhedonia Scale (RPAS) and the Revised Social Anhedonia Scale (RSAS) have been applied in clinical and non-clinical samples since 1980s. However, the construct of a unified RPAS&RSAS for comprehensive measurement of anhedonia has never been explored. Therefore, the purpose of our study was to examine the factor structure of the unified RPAS&RSAS among undergraduates and clinical patients. METHODS: A total of 3435 undergraduates from two universities and 294 clinical patients with mental disorders had completed the Chinese version of the RPAS and the RSAS. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were each conducted to reveal the constructs of the RPAS and the RSAS. CFA was used to evaluate first- and second-order models for the unified RPAS&RSAS in undergraduates and clinical patients. The internal consistency and test-retest reliability of the RPAS and the RSAS were also evaluated. RESULTS: EFA and CFA indicated 2-factor structures for RPAS and RSAS, with the factors being defined as anticipatory anhedonia and consummatory anhedonia. The second-order model of the unified RPAS&RSAS in the undergraduates and clinical patients both had satisfactory fit index values (Undergraduate sample: CFI = 0.901, TLI = 0.899, RMSEA = 0.055, SRMR = 0.086; Clinical sample: CFI = 0.922, TLI = 0.911, RMSEA = 0.052, SRMR = 0.078). The psychometric robustness of the RPAS&RSAS were confirmed by high internal consistency and test-retest reliability values. CONCLUSIONS: The unified RPAS&RSAS with a second-order structure was confirmed in both undergraduates and clinical samples in Chinese. The construct of anhedonia was refreshed as covering physical and social domains, and each of them includes both anticipatory and consummatory components.

6.
World J Gastroenterol ; 26(40): 6250-6259, 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33177797

RESUMO

BACKGROUND: Pancreatic adenoma can potentially transform into adenocarcinoma, so it is recommended to be resected surgically or endoscopically. Endoscopic papillectomy is one of the main treatments for papillary adenoma, and bleeding, perforation, and pancreatitis are the most frequent and critical adverse events that restrict its wider use. There is no standard procedure for endoscopic papillectomy yet. The procedure is relevant to postoperative adverse events. AIM: To reduce the postoperative adverse event rates and improve patients' postoperative condition, we developed a standard novel procedure for endoscopic papillectomy. METHODS: The novel endoscopic papillectomy had two main modifications based on the conventional method: The isolation of bile from pancreatic juice with a bile duct stent and wound surface protection with metal clips and fibrin glue. We performed a single-center retrospective comparison study on the novel and conventional methods to examine the feasibility of the novel method for reducing postoperative adverse events. RESULTS: A total of 76 patients, of whom 23 underwent the novel procedure and 53 underwent the conventional procedure, were retrospectively evaluated in this study. The postoperative bleeding and pancreatitis rates of the novel method were significantly lower than those of the conventional method (0 vs 20.75%, P = 0.028, and 17.4% vs 41.5%, P = 0.042, respectively). After applying the novel method, the most critical adverse event, perforation, was entirely prevented, compared to a prevalence of 5.66% with the conventional method. Several postoperative symptoms, including fever, rapid pulse, and decrease in hemoglobin level, were significantly less frequent in the novel group (P = 0.042, 0.049, and 0.014, respectively). Overall, the total adverse event rate of the novel method was lower (0 vs 24.5%, P = 0.007) than that of the conventional method. CONCLUSION: Patients who underwent the novel procedure had lower postoperative adverse event rates. This study demonstrates the potential efficacy and safety of the novel endoscopic papillectomy in reducing postoperative adverse events.

7.
Minerva Pediatr ; 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33182993

RESUMO

BACKGROUND: Intracranial hemorrhage is a severe cranial disease in the perinatal period. We aimed to explore the feasibility and accuracy of three-dimensional (3D) ultrasonography for the quantitative evaluation of neonatal intracranial hemorrhage. METHODS: A total of 374 neonates with suspected intracranial hemorrhage from January 2017 to December 2019 were selected to be primarily screened by cranial ultrasonography and then diagnosed by cranial CT scan. The examination results were compared to analyze the feasibility and accuracy of 3D ultrasonography in quantifying blood loss. RESULTS: CT scan showed that there were 102 cases of Papile grade I, 106 cases of grade II, 124 cases of grade III and 42 cases of grade IV. 3D ultrasonography showed that there were 108 cases of Papile grade I, 98 cases of grade II, 130 cases of grade III and 38 cases of grade IV. The diagnostic results of these two methods were not significantly different (P>0.05). The accuracies of CT scan for subventricular, intraventricular, subdural, subarachnoid and intraparenchymal hemorrhages were 47.33%, 31.24%, 94.62%, 91.73% and 91.35% respectively, and those of 3D ultrasonography were 98.74%, 96.37%, 91.51%, 90.41% and 97.64% respectively. The accuracies of 3D ultrasonography were significantly superior to those of CT scan for subependymal, intraventricular and intraparenchymal hemorrhages (P<0.05). CONCLUSIONS: Neonatal intracranial hemorrhage can be well diagnosed by cranial ultrasonography which timely provides evidence for clinicians, thereby elevating the cure rate and reducing the mortality rate and incidence rate of sequelae. 3D ultrasonography is feasible and accurate for the quantitative evaluation of neonatal intracranial hemorrhage, thus being of great significance to prognostic determination in clinical practice.

8.
J Transl Med ; 18(1): 432, 2020 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-33183315

RESUMO

Extracellular vesicles are heterogeneous populations of naturally occurring secreted small vesicles. EVs function as signaling platforms to facilitate intracellular communication, which indicates the physiological or pathophysiological conditions of cells or tissues. Considering that EVs can be isolated from most body fluids and that molecular constituents could be reprogrammed according to the physiological status of the secreting cells, EVs are regarded as novel diagnostic and prognostic biomarkers for many diseases. The ability to protect encapsulated molecules from degradation in body fluids suggests the potential of EVs as biological medicines or drug delivery systems. This article focuses on the EV-associated biomarkers and therapeutic approaches in autoimmune diseases.

9.
J Coll Physicians Surg Pak ; 30(11): 1161-1165, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33222732

RESUMO

OBJECTIVE: To compare the superior mesenteric artery (SMA) and inferior mesenteric artery (IMA) diameters in colorectal cancer compared to control. STUDY DESIGN: Observational study. PLACE AND DURATION OF STUDY: Taizhou Hospital, Zhejiang University, Taizhou, China, from March 2019 to June 2020. METHODOLOGY: A total of 203 diagnosed colorectal cancer patients and 40 patients as control subjects were reviewed. Patients were divided into three groups based on tumor location as the right colon, left colon, and rectal groups. The diameters were measured on axial computed tomography images independently by two observers. RESULTS: The SMA diameter did not differ between the right colon and control groups (p=0.626). The IMA diameter was significantly higher in the left colon group than in the control group (p=0.002), but there was no significant difference in the IMA diameter between the different tumour stages (p=0.263). The IMA diameter was significantly higher in the rectal group than in the control group (p<0.001). There was a significant increase in the IMA diameter from stage I to stage II rectal cancers (p=0.022) and from stage II to stage III rectal cancers (p=0.003). The IMA diameter did not differ between stage III and IV rectal cancers (p=0.600). In locoregional rectal cancer patients, there was a significant correlation between the IMA diameter and tumour-node-metastasis stage (p<0.001, rs = 0.494). CONCLUSION: Patients with rectal cancer and left colon cancer have a wider IMA diameter than patients without colorectal cancer. IMA diameter can be a potential marker for locoregional staging of rectal cancer. Key Words: Superior mesenteric artery, Inferior mesenteric artery, Colorectal cancer, Markers, Diameter.

10.
BMJ Open ; 10(11): e042573, 2020 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-33172950

RESUMO

OBJECTIVE: To analyse the incidence, risk factors and impact of acute kidney injury (AKI) on the prognosis of patients with COVID-19. DESIGN: Meta-analysis. DATA SOURCES: PubMed, Embase, CNKI and MedRxiv of Systematic Reviews from 1 January 2020 to 15 May 2020. STUDY SELECTION: Studies examining the following demographics and outcomes were included: patients' age; sex; incidence of and risk factors for AKI and their impact on prognosis; COVID-19 disease type and incidence of continuous renal replacement therapy (CRRT) administration during COVID-19 infection. RESULTS: A total of 79 research articles, including 49 692 patients with COVID-19, met the systemic evaluation criteria. The mortality rate and incidence of AKI in patients with COVID-19 in China were significantly lower than those in patients with COVID-19 outside China. A significantly higher proportion of patients with COVID-19 from North America were aged ≥65 years and also developed AKI. European patients with COVID-19 had significantly higher mortality and a higher CRRT rate than patients from other regions. Further analysis of the risk factors for COVID-19 combined with AKI showed that age ≥60 years and severe COVID-19 were independent risk factors for AKI, with an OR of 3.53, 95% CI (2.92-4.25) and an OR of 6.07, 95% CI (2.53-14.58), respectively. The CRRT rate in patients with severe COVID-19 was significantly higher than in patients with non-severe COVID-19, with an OR of 6.60, 95% CI (2.83-15.39). The risk of death in patients with COVID-19 and AKI was significantly increased, with an OR of 11.05, 95% CI (9.13-13.36). CONCLUSION: AKI was a common and serious complication of COVID-19. Older age and having severe COVID-19 were independent risk factors for AKI. The risk of in-hospital death was significantly increased in patients with COVID-19 complicated by AKI.


Assuntos
Lesão Renal Aguda/epidemiologia , Infecções por Coronavirus/fisiopatologia , Mortalidade Hospitalar , Pneumonia Viral/fisiopatologia , Lesão Renal Aguda/etiologia , Lesão Renal Aguda/terapia , Fatores Etários , Betacoronavirus , China/epidemiologia , Terapia de Substituição Renal Contínua , Infecções por Coronavirus/complicações , Infecções por Coronavirus/terapia , Europa (Continente)/epidemiologia , Humanos , América do Norte/epidemiologia , Pandemias , Pneumonia Viral/complicações , Pneumonia Viral/terapia , Prognóstico , Fatores de Risco , Índice de Gravidade de Doença , Fatores Sexuais
11.
J Chromatogr A ; 1634: 461666, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33197846

RESUMO

Off-line two-dimensional countercurrent chromatography has been widely applied to the isolation of complex samples, but little research on the investigation of orthogonality in the selection of biphasic solvent systems is available. In the present work, the orthogonality in the selection of a biphasic solvent system for liquid-liquid chromatographic separation of aqueous extract and ether extract from the traditional Chinese medicinal plant Polygonum cuspidatum Sieb. et Zucc was evaluated by the correlation coefficient and space occupancy rate. In total, 25 different biphasic solvent systems were tested, and 313 system combinations were analysed. A convex hull methodology was used to determine the separation space and to optimize separation conditions. The correlation coefficient matrix was transformed into dendrograms and a colour map to visualize the dissimilarity between, and orthogonality for, all solvent systems. The aqueous extracts from Polygonum cuspidatum were separated using selected biphasic solvent systems with high orthogonality: ethyl acetate-ethanol-water (70:1:70, v/v) and petroleum ether-ethyl acetate-water (1:5:5, v/v). The ether extracts from Polygonum cuspidatum were also separated using selected biphasic solvent systems with high orthogonality: petroleum-ethyl acetate-methanol-aqueous 0.25 M NH3•H2O (5:5:5:5, v/v) and petroleum-ethyl acetate-methanol-water (5:5:5:5, v/v). Thirteen compounds were successfully obtained. The experimental results demonstrated that the evaluation of orthogonality provided an alternative strategy to select an applicable solvent system for the separation of complex samples using off-line two-dimensional countercurrent chromatography.

12.
J Foot Ankle Surg ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33218860

RESUMO

Distal metaphyseal tibial fractures (3-5 cm from the joint with zones of comminution or <3 cm from the joint) are challenging to fix and are associated with many complications. The study objective was to evaluate the functional outcomes and complications after treating distal metaphyseal tibial fractures using anatomical anterolateral tibia locking plates or anterolateral-medial plates. This retrospective study included 57 patients with distal metaphyseal tibial fractures. Thirty patients were treated by open reduction internal fixation with anterolateral plates; 27 patients were treated with anterolateral-medial plates. Patients were followed at regular intervals. The time to fracture union and complications were recorded. We evaluated the stage of fracture healing using the Radiographic Union Score for Tibial fractures. The patients treated with anterolateral plates had significantly higher rates of loss of reduction and malunion than those treated with anterolateral-medial plates (p = .02 and p = .002, respectively). There were no significant differences in the radiographic union scores (p = .22), non-union (p = .17), incision necrosis (p = .91), or infection (p = .94) between the 2 groups. The functional outcomes were assessed using the American Orthopedic Foot and Ankle Society hindfoot-ankle score at the 12-month follow-up. The mean hindfoot-ankle scores were 90.9 ± 5.0 (range 79 to 100, median 90) and 92.3 ± 5.1 (range 82 to 100, median 92) for the anterolateral plates and anterolateral-medial plates, respectively (p = .29). For distal metaphyseal tibial fractures, anterolateral-medial plates may be worthwhile for reducing loss of reduction and malunion.

13.
World J Gastroenterol ; 26(41): 6431-6441, 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33244203

RESUMO

BACKGROUND: Endoscopic drainage of walled-off necrosis (WON) is still a challenge due to stent-associated problems. We explored endoscopic gastric fenestration (EGF) as an innovative alternative intervention. AIM: To assess the feasibility, efficacy and safety of EGF for WON. METHODS: Between March 2019 and March 2020, five patients with symptomatic WON in close contact with the stomach wall were treated by EGF. Endoscopic ultrasound (EUS) was used to select appropriate sites for gastric fenestration, which then proceeded layer by layer as in endoscopic submucosal dissection. Both the stomach muscularis propria and pseudocyst capsule were penetrated. Fenestrations were expanded up to 1.5-3 cm for drainage or subsequent necrosectomy. RESULTS: EGF failed in Case 1 due to nonadherence of WON to the gastric wall. EGF was successfully implemented in the other four cases by further refinement of fenestration site selection according to computed tomography, endoscopy and EUS features. The average procedure time for EGF was 124 min (EUS assessment, 32.3 min; initial fenestration, 28.8 min; expanded fenestration, 33 min), and tended to decrease as experience gradually increased. The diameter of the fenestration site was 1.5-3 cm, beyond the caliber of a lumen-apposing metal stent (LAMS), to ensure effective drainage or subsequent necrosectomy. Fenestration sites showed surprising capacity for postoperative self-healing within 1-3 wk. No EGF-related complications were seen. WON disappeared within 3 wk after EGF. In Case 3, another separate WON, treated by endoscopic LAMS drainage, recurred within 4 d after LAMS removal due to stent-related hemorrhage, and resolved slowly over almost 3 mo. No recurrences were observed in the five patients. CONCLUSION: EGF is an innovative and promising alternative intervention for WON adherent to the gastric wall. The challenge resides in the gauging of actual adherence and in selecting appropriate fenestration sites.

14.
Artigo em Inglês | MEDLINE | ID: mdl-33246326

RESUMO

OBJECTIVES: While myeloid-derived suppressor cells (MDSCs) were previously shown to promote a proinflammatory T helper (Th) 17 response in autoimmune conditions, a potential impact of the MDSC-Th17 immune axis on abnormal bone destruction in RA remains largely unknown. METHODS: We investigated the correlation between the frequency of MDSCs or its subsets and joint destruction in RA patients. The reciprocal actions of patient-derived MDSCs and Th17 cells were studied using osteoclast (OC) differentiation and bone resorption assays in vitro, which were further validated using mouse models of RA. Contribution of MDSCs to osteoclastogenesis and bone erosion in vivo was determined by depletion or transfer of MDSCs. RESULTS: Human MDSCs, particularly monocytic MDSCs (M-MDSCs), exhibit inherent OC-differentiating capacity and positively correlate with clinical bone erosion in RA patients. Strikingly, patient-derived M-MDSCs can program Th17 cells towards a pro-osteoclastogenic phenotype, which in return potentiates OC differentiation via the receptor activator of nuclear factor κΒ ligand (RANK-L)-RANK signalling. This enhanced osteolysis driven by the reciprocal actions of M-MDSCs and Th17 cells is further confirmed using mouse models of RA. Selective depletion of M-MDSCs significantly ameliorates osteoclastogenesis and disease severity in arthritic mice, whereas transfer of M-MDSCs aggravates bone erosion associated with increased OCs in recipient mice. CONCLUSION: Our findings highlight the functional plasticity of MDSCs and identify a novel pro-osteoclastogenic pathway governed by interplay between myeloid cells and T lymphocytes in autoimmune RA.

15.
J Sep Sci ; 2020 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-33247875

RESUMO

2-(4-chlorophenyl) succinic acid was successfully enantioseparated by countercurrent chromatography using hydroxypropyl-ß-cyclodextrin as chiral selector. A two-phase solvent system composed of n-hexane-ethyl acetate-0.1 mol/L phosphate buffer with pH 2.65 (5:5:10, v/v/v) was selected. Enantioselective liquid-liquid extraction was used to optimize the enantioseparation conditions. Meanwhile, the influence of injection volume on resolution in countercurrent chromatography was investigated and a linear relationship between the inflection point of injection volume and sample loading was tentatively obtained. The peak resolution will decrease significantly when the injection volume over the inflection point was used. In addition, it could be found that the smaller amount of sample loading, the larger impact of injection volume on resolution could be observed, which might serve as a good reference for the selection of sample volume in enantioseparations by countercurrent chromatography. Under optimized conditions, 20 mg of 2-(4-chlorophenyl) succinic acid racemate dissolved in 10 mL of aqueous phase was successfully enantioseparated by countercurrent chromatography. The recovery for both of the enantiomer of (±)-2-(4-chlorophenyl) succinic acid reached more than 70%-75% with a purity of 99.0%. This article is protected by copyright. All rights reserved.

16.
Artigo em Inglês | MEDLINE | ID: mdl-33243710

RESUMO

The limited availability of nutrient Fe severely impairs the health of almost all organisms. Endophytic actinobacteria can benefit the host plant in different ways. We previously inferred that the rice (Oryza) endophytic Streptomyces hygroscopicus OsiSh-2 possesses a highly efficient Fe-acquisition system. In this work, we first evaluated the effects of OsiSh-2 on the Fe-deficiency resilience of the host rice. The results demonstrated that the inoculation of OsiSh-2 considerably increased the plant biomass, Fe concentration and translocation factor, and chlorophyll content, and net leaf photosynthetic rate under Fe limiting condition. The expression of genes involved with Fe3+-reduction-related strategy in rice was up-regulated, while that involved with Fe3+-chelation-related strategy was down-regulated by OsiSh-2 treatment. Meanwhile, the OsiSh-2-rice symbiont showed enhancement of Fe3+-chelate reductase activity, total siderophore production, and acidification trend in the rhizosphere under Fe deficiency compared to plants without this endophyte. In conclusion, endophytic OsiSh-2 could protect plants against Fe-deficient stress by a sophisticated interaction with the host, including modulating Fe chelation, solubilization, reduction and translocation, ultimately leading to enhanced fitness of plant.

17.
Sheng Wu Gong Cheng Xue Bao ; 36(11): 2334-2344, 2020 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-33244928

RESUMO

Strain variability is one of the most important factors to influence the accuracy of foodborne pathogens risk assessment, such as Listeria monocytogenes, Salmonella spp. Strain-to-strain variation is defined as the inherent differences among identically treated strains of the same microbial species. The differences cannot be eliminated by changing test methods or improving test protocols. This review addresses presently related studies of strain variability. Based on the effect of strain variability on the outcome of risk assessment, we summarize sources of variabilities in food chain, strain phenotypic variabilities and the methods to integrate strain variability in growth and inactivation into predictive modelling, and indicate the inadequacies in the study of strain variability. We suggest further study the mechanism of strain variability, expand the comparison of variability among different sources, and integrate the variability of gene expression, protein and cell metabolism into the predictive modelling.

18.
Mol Med ; 26(1): 112, 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33225891

RESUMO

BACKGROUND: The oncogenic role of the newly identified lncRNA LUADT1 has been revealed in lung adenocarcinoma. It was reported that LUADT1 plays a critical role in multiple human diseases. This study was carried out to investigate the role of LUADT1 in sepsis. METHODS: Sixty patients with sepsis and sixty healthy volunteers were recruited for this study. Plasma samples were collected from all participants. Human primary coronary artery endothelial cells were also used in this study. The expression of Pim-1, miR-195 and LUADT1 were detected by RT-qPCR. The interaction between miR-195 and LUADT1 was determined by overexpression experiments and luciferase activity assay. Cell apoptosis was detected by flow cytometry. The expression of apoptosis-related protein was detected by Western blotting. RESULTS: Bioinformatics analysis revealed the potential interaction between LUADT1 and miR-195, which was confirmed by dual luciferase reporter assay. LUADT1 was downregulated in patients with sepsis. Moreover, LPS treatment downregulated the expression of LUADT1 in primary cardiac endothelial cells. Overexpression of LUADT1 and miR-195 did not affect the expression of each other in primary cardiac endothelial cells. Interestingly, overexpression of LUADT1 was found to upregulate the expression of Pim-1, a target of miR-195. In addition, it was found that overexpression of LUADT1 and Pim-1 reduced the enhancement effects of miR-195 on LPS-induced cardiac endothelial cell apoptosis. CONCLUSION: In summary, LUADT1 may protect cardiac endothelial cells against apoptosis in sepsis by regulating the miR-195/Pim-1 axis.

19.
Anal Chem ; 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33237721

RESUMO

Surface-enhanced Raman spectroscopy (SERS) is a powerful tool to monitor various interfacial behaviors providing molecular level information with high spatial and temporal resolutions. However, it is a challenge to obtain SERS spectra with high quality for analytes having a weak binding affinity with plasmonic nanostructures due to the short dwell time of the analyte on the surface. Here, we employed dynamic SERS, an acquisition method consisting of the rapid acquisition of a series of consecutive SERS spectra, to study the adsorption/desorption behavior of R6G on Ag surfaces. We demonstrated that the signal-noise ratio of SERS spectra of mobile molecules can be improved by dynamic SERS even when the acquisition time cannot catch up with the diffusion time of the molecule. More interestingly, we captured the neutral R6G0 state (spectroscopically different from the dominated positive R6G+ state) of R6G at the single-molecule level, which is a rare molecule event hardly detectable by traditional SERS. Dynamic SERS provides near real-time molecular vibrational information with an improved signal-noise ratio, which opens a new avenue to capture metastable or rare molecule events for the comprehensive understanding of interfacial processes related to catalysis and life science.

20.
Chem Commun (Camb) ; 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33227104

RESUMO

The reaction of cyclic organobismuth compounds, 12-phenyl- and 12-chloro-5,6,7,12-tetrahydrodibenz[c,f][1,5]azabismocines, with Pt(PEt3)3 was examined. Oxidative addition of the exocyclic Bi-C bond to Pt(0) selectively took place in the reaction of the 12-phenyl derivative. Oxidative addition of the exocyclic Bi-Cl bond reversibly took place and was kinetically preferred, while endocyclic Bi-C bond oxidative addition products were thermodynamically favored and became the final products in the reaction of 12-chloro derivatives. These results demonstrate the oxidative addition of a Bi-C bond to a transition metal complex for the first time.

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