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1.
Ultrason Sonochem ; 93: 106301, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36669430

RESUMO

In this paper, the cavitation bubble dynamics near two spherical particles of the same size are investigated theoretically and experimentally. According to the Weiss theorem, the flow characteristics and the Kelvin impulse are obtained and supported by the sufficient experimental data. In terms of the initial bubble position, the bubble size and the distance between the two particles, the collapse morphology and the movement characteristics of the bubble are revealed in detail. The main findings include: (1) Based on a large number of experimental results, it is found that the Kelvin impulse theoretical model established in this paper can effectively predict the movement characteristics of the cavitation bubble near two particles of the same size. (2) When the initial bubble position is gradually away from the particles along the horizontal symmetry axis near two particles of the same size, the movement distance of the bubble centroid in the first period increases first and then decreases. (3) When the initial position of the bubble centroid is at the asymmetric position near the two particles, the movement direction of the bubble centroid is biased towards the particle closer to the bubble, but not towards the center of this particle.

2.
Biosensors (Basel) ; 13(1)2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36671957

RESUMO

The accurate, simple, and efficient measurement of the concentration of single-stranded DNA (ssDNA) is important for many analytical applications, such as DNA adsorption, biosensor design, and disease diagnosis, but it is still a challenge. Herein, we studied a cationic conjugated polymer (CCP)-based ssDNA assay taking advantage of the obvious fluorescence change of CCPs upon binding ssDNA. Poly(3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene hydrochloride) (PMNT) achieved an apparent dissociation constant (Kd) of 57 ± 4 nM for ssDNA, indicating a very high binding affinity between PMNT and ssDNA. This allowed us to develop a CCP-based ssDNA biosensor with a detection limit of 0.6 nM, similar to the fluorescence-dye-based method using SYBR Green I and SYBR Gold. Our CCP-based biosensor produced smaller differences among ssDNA samples with different base compositions. In addition, the existence of double-stranded DNA (dsDNA) at different concentrations did not interfere with the fluorescence of PMNT, indicating that our CCP-based biosensor was more suitable for the measurement of ssDNA. Compared with fluorescence-intensity-based quantification, our CCP system allowed ratiometric quantification, which made the calibration easier and more robust. We then applied our method to the quantification of ssDNA on AuNPs using both unmodified and thiolated ssDNA, and the accurate quantification of ssDNA was achieved without any fluorophore modification. This method provides an alternative approach for the measurement of ssDNA.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Polímeros/química , Ouro , DNA/química , DNA de Cadeia Simples , Cátions/química , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química
3.
ACS Nano ; 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36598186

RESUMO

The development of nanosystems with intrinsic immunomodulatory effects on macrophage polarization is important for the macrophage-targeted immunotherapy. Here, mitochondria-targeted bovine serum albumins (BSAs) via the conjugation of fluorescent, lipophilic, and cationic rhodamine 110 molecules can efficiently enhance the gene expression of the proinflammatory phenotype of macrophages and correspondingly inhibit the gene expression of their anti-inflammatory phenotype. On this basis, porous silicon nanocarriers can further boost the immunomodulation of these mitochondria-targeted BSAs in vitro or in vivo, accompanied by the secretion of proinflammatory mediators including tumor necrosis factor α, nitric oxide, and reactive oxygen species (ROS). Meanwhile, BSA coatings can also improve the biocompatibility of porous silicon nanoparticulate cores on macrophages. Finally, the mechanism investigations demonstrate that porous silicon nanocarriers can efficiently deliver mitochondria-targeted BSA into macrophages to generate mitochondrial ROS via the interference with mitochondrial respiratory chains, which can further trigger the downstream signaling transduction pathways for the proinflammatory transition. Considering the good biosafety and versatile loading capability, this developed porous silicon@BSA nanosystem with a strong proinflmmatory regulatory effect has important potential on the combinatorial chemoimmunotherapy against cancer or viral/bacterial-related infectious diseases.

4.
J Zhejiang Univ Sci B ; 24(1): 32-49, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36632749

RESUMO

Hypoxia, as an important hallmark of the tumor microenvironment, is a major cause of oxidative stress and plays a central role in various malignant tumors, including glioblastoma. Elevated reactive oxygen species (ROS) in a hypoxic microenvironment promote glioblastoma progression; however, the underlying mechanism has not been clarified. Herein, we found that hypoxia promoted ROS production, and the proliferation, migration, and invasion of glioblastoma cells, while this promotion was restrained by ROS scavengers N-acetyl-L-cysteine (NAC) and diphenyleneiodonium chloride (DPI). Hypoxia-induced ROS activated hypoxia-inducible factor-1α (HIF-1α) signaling, which enhanced cell migration and invasion by epithelial-mesenchymal transition (EMT). Furthermore, the induction of serine protease inhibitor family E member 1 (SERPINE1) was ROS-dependent under hypoxia, and HIF-1α mediated SERPINE1 increase induced by ROS via binding to the SERPINE1 promoter region, thereby facilitating glioblastoma migration and invasion. Taken together, our data revealed that hypoxia-induced ROS reinforce the hypoxic adaptation of glioblastoma by driving the HIF-1α-SERPINE1 signaling pathway, and that targeting ROS may be a promising therapeutic strategy for glioblastoma.


Assuntos
Glioblastoma , Humanos , Glioblastoma/metabolismo , Glioblastoma/patologia , Espécies Reativas de Oxigênio/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Transdução de Sinais , Hipóxia , Linhagem Celular Tumoral , Hipóxia Celular , Microambiente Tumoral , Inibidor 1 de Ativador de Plasminogênio/metabolismo
5.
Sci Total Environ ; 869: 161741, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36693574

RESUMO

Excessive vanadium (V) contamination is an attracting growing concern, which can negatively affect the health of human and ecosystems. But how V causes nephrotoxicity and the role of mitochondria-associated endoplasmic reticulum membrane (MAM) in V-induced nephrotoxicity have remained elusive. To explore the detailed mechanism and screen of potential effective drugs for V-evoked nephrotoxicity, a total of 72 ducks were divided into two groups, control group and V group (30 mg/kg V). Results showed that excessive V damaged kidney function of ducks including causing histopathological abnormality, biochemical makers derangement and oxidative stress. Then MAM of duck kidneys was extracted to investigate differentially expressed proteins (DEPs) under V exposure using proteomics analysis. Around 4240 MAM-localized proteins were identified, of which 412 DEPs showed dramatic changes, including 335 upregulated and 77 downregulated DEPs. On the basis of gene ontology (GO), string and KEGG database analysis, excessive V led to nephrotoxicity primarily by affecting MAM-mediated metabolic pathways, especially elevating the endoplasmic Reticulum (ER) proteostasis related pathway. Further validation analysis of the detected genes and proteins of ER proteostasis related pathway under V poisoning revealed a consistent relationship with proteome analysis, indicating that V disrupted MAM-mediated ER proteostasis. Accordingly, our data proved the critical role for MAM in V-evoked nephrotoxicity, particularly with MAM-mediated ER proteostasis, providing promising insights into the toxicological exploration mechanisms of V.

6.
Plant J ; 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36705084

RESUMO

Ribosome biogenesis is a process of making ribosomes that is tightly linked with plant growth and development. Here, through a suppressor screen for the smo2 mutant, we found that lack of a ribosomal stress response mediator, ANAC082 partially restored growth defects of the smo2 mutant, indicating SMO2 is required for the repression of nucleolar stress. Consistently, the smo2 knock-out mutant exhibited typical phenotypes characteristic of ribosome biogenesis mutants, such as pointed leaves, aberrant leaf venation, disrupted nucleolar structure, abnormal distribution of rRNA precursors and enhanced tolerance to aminoglycoside antibiotics that target ribosomes. SMO2 interacted with ROOT INITIATION DEFECTIVE 2 (RID2), a methyltransferase-like protein required for pre-rRNA processing. SMO2 enhanced RID2 solubility in E. coli and the loss of function of SMO2 in plant cells reduced RID2 abundance, which may result in abnormal accumulation of FIBRILLARIN 1 (FIB1) and NOP56, two key nucleolar proteins, in high-molecular-weight protein complex. Taken together, our results characterized a novel plant ribosome biogenesis factor, SMO2 that maintains the abundance of RID2, thereby sustaining ribosome biogenesis during plant organ growth.

7.
J Funct Biomater ; 14(1)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36662091

RESUMO

To explore the potential applicability of chitosan (CTS), we prepared aldehyde chitosan (CTS-CHO) with chitosan and sodium periodate via oxidation reaction and then a chitosan-based hydrophilic and antibacterial coating on the surface of poly (lactic acid) (PLA) film was developed and characterized. The oxidation degree was determined by Elemental analyser to be 12.53%, and a Fourier transform infrared spectroscopy was used to characterize the structure of CTS-CHO. It was evident that CTS-CHO is a biocompatible coating biomaterial with more than 80% cell viability obtained through the Live/Dead staining assay and the alamarBlue assay. The hydrophilic and antibacterial CTS-CHO coating on the PLA surface was prepared by ultrasonic atomization assisted LbL assembly technique due to Schiff's base reaction within and between layers. The CTS-CHO coating had better hydrophilicity and transparency, a more definite industrialization potential, and higher antibacterial activity at experimental concentrations than the CTS coating. All of the results demonstrated that the ultrasonic atomization-assisted LbL assembly CTS-CHO coating is a promising alternative for improving hydrophilicity and antibacterial activity on the PLA surface. The functional groups of CTS-CHO could react with active components with amino groups via dynamic Schiff's base reaction and provide the opportunity to create a drug releasing surface for biomedical applications.

8.
Anal Methods ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36688606

RESUMO

In this study, 3-carboxyphenylboronic acid (CP)-functionalized amino-modified Fe3O4 (Fe3O4@NH2-CP, FNC) magnetic molecularly imprinted polymers (FNC@MIPs) were synthesized and applied for the quick identification and selective separation of luteolin (LTL). The structure and morphology were characterized in detail by Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), dynamic light scattering (DLS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and vibrating specimen magnetometry (VSM) methods. The FNC@MIPs had a homogeneous shape, excellent magnetic characteristics, quick binding kinetics, a high adsorption capacity, acceptable selectivity, and stable reusability. The solid-phase extraction parameters and preparation conditions were both optimized. Under optimized conditions, the maximal adsorption capacity was 14.26 mg g-1 and the imprinting factor was 3.62. Furthermore, the experimental kinetics data were best fitted with the pseudo-first-order model (R2 = 0.9877), and the Langmuir model could describe the adsorption process (R2 = 0.9979), suggesting a monolayer covering. The practical application of the sorbent for LTL detection in Lonicera japonica Thunb samples showed recoveries in the range of 84.5-108.7%. Therefore, the strategy offers a fresh avenue for the extraction and purification of LTL.

9.
Plant Methods ; 19(1): 7, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36691062

RESUMO

BACKGROUND: Karst vegetation is of great significance for ecological restoration in karst areas. Vegetation Indices (VIs) are mainly related to plant yield which is helpful to understand the status of ecological restoration in karst areas. Recently, karst vegetation surveys have gradually shifted from field surveys to remote sensing-based methods. Coupled with the machine learning methods, the Unmanned Aerial Vehicle (UAV) multispectral remote sensing data can effectively improve the detection accuracy of vegetation and extract the important spectrum features. RESULTS: In this study, UAV multispectral image data at flight altitudes of 100 m, 200 m, and 400 m were collected to be applied for vegetation detection in a karst area. The resulting ground resolutions of the 100 m, 200 m, and 400 m data are 5.29, 10.58, and 21.16 cm/pixel, respectively. Four machine learning models, including Random Forest (RF), Support Vector Machine (SVM), Gradient Boosting Machine (GBM), and Deep Learning (DL), were compared to test the performance of vegetation coverage detection. 5 spectral values (Red, Green, Blue, NIR, Red edge) and 16 VIs were selected to perform variable importance analysis on the best detection models. The results show that the best model for each flight altitude has the highest accuracy in detecting its training data (over 90%), and the GBM model constructed based on all data at all flight altitudes yields the best detection performance covering all data, with an overall accuracy of 95.66%. The variables that were significantly correlated and not correlated with the best model were the Modified Soil Adjusted Vegetation Index (MSAVI) and the Modified Anthocyanin Content Index (MACI), respectively. Finally, the best model was used to invert the complete UAV images at different flight altitudes. CONCLUSIONS: In general, the GBM_all model constructed based on UAV imaging with all flight altitudes was feasible to accurately detect karst vegetation coverage. The prediction models constructed based on data from different flight altitudes had a certain similarity in the distribution of vegetation index importance. Combined with the method of visual interpretation, the karst green vegetation predicted by the best model was in good agreement with the ground truth, and other land types including hay, rock, and soil were well predicted. This study provided a methodological reference for the detection of karst vegetation coverage in eastern China.

10.
J Am Chem Soc ; 145(3): 1696-1706, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36622785

RESUMO

Inspired by the synthesis of XB3C3 (X = Sr, La) compounds in the bipartite sodalite clathrate structure, density functional theory (DFT) calculations are performed on members of this family containing up to two different metal atoms. A DFT-chemical pressure analysis on systems with X = Mg, Ca, Sr, Ba reveals that the size of the metal cation, which can be tuned to stabilize the B-C framework, is key for their ambient-pressure dynamic stability. High-throughput density functional theory calculations on 105 Pm3̅ symmetry XYB6C6 binary-guest compounds (where X, Y are electropositive metal atoms) find 22 that are dynamically stable at 1 atm, expanding the number of potentially synthesizable phases by 19 (18 metals and 1 insulator). The density of states at the Fermi level and superconducting critical temperature, Tc, can be tuned by changing the average oxidation state of the metal atoms, with Tc being highest for an average valence of +1.5. KPbB6C6, with an ambient-pressure Eliashberg Tc of 88 K, is predicted to possess the highest Tc among the studied Pm3̅n XB3C3 or Pm3̅ XYB6C6 phases, and calculations suggest it may be synthesized using high-pressure high-temperature techniques and then quenched to ambient conditions.

11.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 54(1): 108-113, 2023 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-36647652

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing global pandemic of coronavirus disease 2019 (COVID-19). Proper nutritional support helps boost the immunity of the human body, strengthen the high-risk populations' defense against SARS-CoV-2, reduce the prevalence of COVID-19, prevent mild cases from developing into severe cases, and reduce the occurrence of adverse symptoms during recovery. Nutritional support is an important guarantee to provide protection against virus infection, promote patient recovery, and improve patient prognosis. Whole nutritional food formulas designed according to the characteristic clinical symptoms of COVID-19 provide patients with comprehensive nutritional support of appropriate nutritional content, which effectively improves the nutritional status of patients and provides strong technical support to improve their quality of survival. During the critical period of COVID-19 prevention and control, more emphasis should be placed on the essential role of nutritional support and the clinical efficacy of nutritional support should be given full play.


Assuntos
COVID-19 , Humanos , COVID-19/prevenção & controle , Estado Nutricional , Fatores de Risco , SARS-CoV-2 , Resultado do Tratamento
12.
J Voice ; 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36631344

RESUMO

OBJECTIVES: To compare the effects of botulinum toxin A injection and local glucocorticoid injection for treating laryngeal contact granuloma and to discuss the indications for both regimens. METHODS: The case data of 50 patients with laryngeal contact granuloma in the outpatient clinic of the Sixth Medical Center of the PLA General Hospital from January 2020 to December 2021 were reviewed, and the patients were divided into the following two groups according to the different treatments received: botulinum toxin A injection or local glucocorticoid injection. Quantitative assessment of the lesion size was performed using Image J software to compare the efficacy in the two groups. RESULTS: On comparing the cure rate between the two groups at 3 months after treatment, 72% of patients in the botulinum toxin A group were cured compared to 40% of patients in the glucocorticoid group (P = 0.023). On comparing the cure rate between the two groups at 6 months after treatment, 88% of patients in the botulinum toxin A group were cured compared to 64% of patients in the glucocorticoid group (P = 0.047). CONCLUSIONS: Botulinum toxin A injection is superior to local glucocorticoid injection, and local glucocorticoid injection is more appropriate for patients who cannot tolerate the adverse effects of botulinum toxin A.

13.
Expert Rev Anticancer Ther ; : 1-7, 2023 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-36645663

RESUMO

OBJECTIVES: To systematically evaluate the prognostic value of the nodal yield in oral squamous cell carcinoma by meta-analysis. METHODS: The meta-analysis was adherence to PRISMA. We searched MEDLINE, Embase, and Cochrane for studies published up to 20 April 2022. We collected evidences from observational studies regarding nodal yield in oral squamous cell carcinoma, and investigated its prognostic value by the routine methods of meta-analysis. RESULTS: From seven studies, there was no significant impact of the lymph node yield on overall survival among patients with oral squamous cell carcinoma cases and <18 lymph nodes (hazard ratio (HR) = 1.019, 95% confidence interval (CI) = 0.786-1.320, p = 0.887), with significant heterogeneity (I2 = 80%). The pooled result indicated that a > 18-lymph node yield was a favorable prognostic factor (HR = 0.786, 95%CI = 0.646-0.956, p = 0.016; I2 = 39%). The lymph node yield was not associated with disease-specific survival (HR = 1.594, 95%CI = 0.996-2.552, p = 0.052; I2 = 81%) or disease-free survival (HR = 1.508, 95%CI = 0.924-2.460, p = 0.100; I2 = 41%). CONCLUSION: A lymph node yield of ≥18 lymph nodes might be a favorable prognostic factor for the overall survival of patients with oral squamous cell carcinoma.

14.
Medicina (Kaunas) ; 59(1)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36676738

RESUMO

Background and Objectives: This study aimed to evaluate whether the addition of hypomethylating agents (HMA) to low-intensity chemotherapy can enhance the clinical efficacy of induction treatment for elderly acute myeloid leukemia (AML) patients who are unsuitable for standard induction therapy. Materials and Methods: This study retrospectively analyzed 117 patients over 60 years old who were initially diagnosed with AML and received low-intensity induction treatment in the Department of Hematology in Anhui provincial hospital from January 2015 to December 2020. Twenty-three patients were excluded, and the remaining 94 patients were divided into two groups according to the selection of induction regimens. Results: Forty-four patients received HMA combined with low-intensity chemotherapy, and the other 50 patients received only low-intensity induction chemotherapy. Forty-three patients (45.7%) obtained complete remission (CR) after the initial induction treatment. The CR rate in the HMA plus low-intensity chemotherapy group was 34.1% (15/44), and in the single low-intensity chemotherapy group was 56.0% (28/50) (p = 0.04). The 30 days cumulative early death rates were 9.1% (95% CI: 3.5-22.4%) in the HMA plus low-intensity chemotherapy group and 6.0% (95% CI: 2.0-17.5%) in the single low-intensity chemotherapy group, respectively (p = 0.59), and the one-year cumulative relapse rates were 21.1% (95% Cl: 9.8-41.9%) and 33.3% (95% Cl: 20.3-51.5%), respectively (p = 0.80). The one-year overall survival (OS) rates for patients in the HMA plus low-intensity chemotherapy group and the single low-intensity chemotherapy group were 37.3% (95% Cl: 23.1-51.5%) and 55.4% (95% Cl: 40.5-67.9%), respectively (p = 0.098), and the one-year event-free survival (EFS) rates were 8.5% (95% Cl: 2.2-20.6%) and 20.6% (95% Cl: 9.1-35.3%), respectively (p = 0.058). Conclusions: This study showed that the addition of HMA to low-intensity induction chemotherapy does not improve prognosis in elderly AML patients who are unsuitable for standard induction chemotherapy.


Assuntos
Quimioterapia de Indução , Leucemia Mieloide Aguda , Humanos , Idoso , Pessoa de Meia-Idade , Estudos Retrospectivos , Leucemia Mieloide Aguda/tratamento farmacológico , Resultado do Tratamento , Prognóstico , Indução de Remissão , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
15.
Appl Opt ; 62(1): 217-226, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36606868

RESUMO

In order to ensure optimal optical performance, primary mirror assembly must be impervious to environmental influences. These environmental influences include gravity, assembly error, and thermal change, under which external loads are imposed on the mirror. The external loads degrade the mirror surface accuracy and cause misalignment between mirrors. In this paper, a tripod flexure with a flexible hinge is designed to alleviate the influence of the external load on the surface accuracy of a 2 m primary mirror. This structure can effectively release the rotational freedom, provide a certain translational flexibility, and yield high axial stiffness. The axial stiffness is used to increase the frequency of the primary mirror assembly. According to the fast optimization model, the derivation of close form compliance equations is developed to characterize the flexibility, and parameter optimization is done to achieve the maximum performance. Then a finite element analysis and test are used to verify the final design. The results show that the index requirements of the 2 m primary mirror have been met.

16.
World Neurosurg ; 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36627017

RESUMO

OBJECTIVE: We aimed to evaluate the risk factors for patients, who had hypertensive intracerebral hemorrhage (HICH)-specific hemorrhage without hypertensive history, to elucidate a novel and detailed understanding. METHODS: We conducted a retrospective review to identify patients diagnosed with hemorrhage in HICH-specific locations without hypertensive history in January 2011 and December 2019 from West China Hospital. A least absolute shrinkage and selector operation (LASSO) algorithm was used to select the optimal prognostic factors, and then we performed a multivariable logistic analysis. To verify the accuracy of the nomogram in predicting patient outcome, we used Harrell's statistics, area under the curve, and a calibration as well as decision curve. RESULTS: The LASSO method, at a tenfold cross-validation for 7-day mortality, 90-day mortality, and 90-day morbidity, was applied to construct the prognosis-predicting models. Both a higher Glasgow Coma Scale score at admission and larger hematoma volume ≥13.64 ml were independently associated with better survival at 7-day and 90-day in multivariate analysis. Lactic dehydrogenase >250 IU/L and neutrophilic granulocyte/lymphocyte ratio in 1 increase were significantly associated with poor outcome at 90 days. Only one factor (Glasgow Coma Scale score at 7 days) influencing 90-day morbidity remained in a LASSO model. CONCLUSIONS: In this study, the Glasgow Coma Scale score, hematoma volume, and other laboratory factors (Lactic dehydrogenase and neutrophilic granulocyte/lymphocyte ratio) were related to survival. Our current findings of the specific location ICH need to be proven by a large randomized controlled trial study.

17.
Adv Mater ; : e2210871, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36645218

RESUMO

Electrochemical deionization has been regarded as one of the promising water treatment technologies. Here, CoAl-layered metal oxide nanosheet intercalated by sodium dodecyl sulfate (SDS) with an enhanced interlayer spacing from 0.76 to 1.33 nm is synthesized and used as an anode. The enlarged interlayer spacing provides an enhanced ion diffusion channel and improves the utilization of the interlayer electroactive sites, while heat treatment transferring layered double hydroxides to layered metal oxides offers additional active oxidation reaction sites to facilitate the electro-sorption rate, contributing to the high salt adsorption capacity (31.78 mg g-1 ) and average salt adsorption rate (3.75 mg g-1 min-1 ) at 1.2 V in 500 mg L-1 NaCl solution. In addition, the excellent long-term cycling stability (92.9%) after 40 cycles proves the strong electronic interaction between SDS and the host layer, which is validated by density functional theory calculations later on. Moreover, the electro-sorption mechanism of layered metal oxides that originated from the reconstruction of the layered structure based on the "memory effect" is revealed according to the XPS peak shifts of Co element. This strategy of expanding the interlayer spacing combined with heat treatment makes layered metal oxides a competitive candidate for electrochemical water deionization. This article is protected by copyright. All rights reserved.

18.
Expert Opin Drug Saf ; : 1-10, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36608279

RESUMO

BACKGROUND: This study aimed to evaluate the efficacy and safety of dapagliflozin as a monotherapy glucose-lowering drug treatment for older adults with diabetes. RESEARCH DESIGN & METHODS: Randomized controlled trial reports were retrieved from PubMed, Embase Cochrane Library, and Web of Science from database inception to 8 May 2021. Publication bias and heterogeneity were assessed using the Cochrane risk-of-bias tool and the Cochrane Q statistic, respectively. RESULTS: Compared with placebo, dapagliflozin as a monotherapy glucose-lowering drug did improve the control of glycosylated hemoglobin and fasting plasma glucose levels in older adults. Our analysis also confirmed that the body weight of older adults was well controlled under treatment of dapagliflozin as a monotherapy glucose-lowering drug. Patients in older adults with diabetes took a higher risk of genital infection and renal impairment or failure after treatment of dapagliflozin. In addition, treatment with dapagliflozin reduced the risk of hypoglycemia, and did not reveal increased risk of urinary tract infection and developing fractures compared to placebo in older adults. CONCLUSIONS: Dapagliflozin as a monotherapy glucose-lowering drug appeared to be an effective treatment for older adults with diabetes, although it might increase risk of genital infection and renal impairment or failure.

19.
Comput Struct Biotechnol J ; 21: 176-184, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36544473

RESUMO

The spatial transcriptome has enabled researchers to resolve transcriptome expression profiles while preserving information about cell location to better understand the complex biological processes that occur in organisms. Due to technical limitations, the current high-throughput spatial transcriptome sequencing methods (known as next-generation sequencing with spatial barcoding methods or spot-based methods) cannot achieve single-cell resolution. A single measurement site, called a spot, in these technologies frequently contains multiple cells of various types. Computational tools for determining the cellular composition of a spot have emerged as a way to break through these limitations. These tools are known as deconvolution tools. Recently, a couple of deconvolution tools based on different strategies have been developed and have shown promise in different aspects. The resulting single-cell resolution expression profiles and/or single-cell composition of spots will significantly affect downstream data mining; thus, it is crucial to choose a suitable deconvolution tool. In this review, we present a list of currently available tools for spatial transcriptome deconvolution, categorize them based on the strategies they employ, and explain their advantages and limitations in detail in order to guide the selection of these tools in future studies.

20.
Neural Regen Res ; 18(5): 933-939, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36254971

RESUMO

Chx10-expressing V2a (Chx10+V2a) spinal interneurons play a large role in the excitatory drive of motoneurons. Chemogenetic ablation studies have demonstrated the essential nature of Chx10+V2a interneurons in the regulation of locomotor initiation, maintenance, alternation, speed, and rhythmicity. The role of Chx10+V2a interneurons in locomotion and autonomic nervous system regulation is thought to be robust, but their precise role in spinal motor regulation and spinal cord injury have not been fully explored. The present paper reviews the origin, characteristics, and functional roles of Chx10+V2a interneurons with an emphasis on their involvement in the pathogenesis of spinal cord injury. The diverse functional properties of these cells have only been substantiated by and are due in large part to their integration in a variety of diverse spinal circuits. Chx10+V2a interneurons play an integral role in conferring locomotion, which integrates various corticospinal, mechanosensory, and interneuron pathways. Moreover, accumulating evidence suggests that Chx10+V2a interneurons also play an important role in rhythmic patterning maintenance, left-right alternation of central pattern generation, and locomotor pattern generation in higher order mammals, likely conferring complex locomotion. Consequently, the latest research has focused on postinjury transplantation and noninvasive stimulation of Chx10+V2a interneurons as a therapeutic strategy, particularly in spinal cord injury. Finally, we review the latest preclinical study advances in laboratory derivation and stimulation/transplantation of these cells as a strategy for the treatment of spinal cord injury. The evidence supports that the Chx10+V2a interneurons act as a new therapeutic target for spinal cord injury. Future optimization strategies should focus on the viability, maturity, and functional integration of Chx10+V2a interneurons transplanted in spinal cord injury foci.

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