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1.
Medicine (Baltimore) ; 98(38): e17275, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31568008

RESUMO

Single nucleotide polymorphisms (SNPs) of the interleukin 28B (IL28B) gene has proven to be associated with the clinical outcome of patients with chronic hepatitis virus B or C (HBV or HCV) infections. However, whether IL28B SNPs have an influence on the risk of hepatocellular carcinoma (HCC) among patients with HBV or HCV infection remains controversial. Therefore, this study aims to determine the association between IL28B polymorphisms and the risk of HCC in individuals with HBV or HCV infection.PubMed, EMBASE, and Chinese National Knowledge Infrastructure (CNKI) databases were used to identify studies meeting the selection requirements using the terms "interleukin 28B", "IFN-lambda-3", "IFNL3", "single nucleotide polymorphisms", "SNPs", "hepatocellular carcinoma", "HCC", "liver cancer".A total of 24 eligible original studies (1 cohort study and 23 case-control studies) involved 20238 individuals (HCC group = 8725 vs control group = 11,513) were included. Both IL28B rs12979860 CC and rs8099917 TT genotypes were significantly associated with a decreased risk of HCC among patients with HBV or HCV infection (OR = 0.71, 95% CI = 0.57-0.88; OR = 0.82, 95% CI = 0.72-0.94, respectively). Egger test and Begg test revealed no' publication bias (P > .05). Sensitivity analyses suggested the robustness of the results in this meta-analysis.Both IL28B rs12979860 CC and rs8099917 TT genotypes are protective factors for the development of HCC among patients with HBV or HCV infection. Future prospective studies examining the impact of IL28B polymorphisms on the risk of HCC and investigating the underlying mechanism for the protective role of IL28B polymorphisms in HCC development are warranted.

2.
J Immunol ; 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31591147

RESUMO

Previous studies of Jak-STAT inhibitors have shown promise in treating kidney diseases. The activation of Jak-STAT components is important in cell fate determination in many cell types, including bone marrow-derived cells, which are important contributors in renal interstitial fibrosis. In this study, we tested the effect of a new STAT3 inhibitor, BP-1-102, on monocyte-to-fibrocyte transition and the progression of renal interstitial fibrosis. We tested the effect of BP-1-102 in a mouse model of unilateral ureteral obstruction in vivo and IL-33-treated bone marrow-derived monocytes in vitro. BP-1-102 treatment alleviated renal interstitial fibrosis, reduced collagen deposition and extracellular matrix protein production, inhibited inflammatory cell infiltration, suppressed the percentage of CD45+ PDGFRß+, CD45+ CD34- Col I+ and CD45+ CD11b+ Col I+ cells within the obstructed kidney and reduced the mRNA levels of the proinflammatory and profibrotic cytokines IL-1ß, TGF-ß, TNF-α, ICAM-1, and CXCL16. In vitro, BP-1-102 inhibited the IL-33-induced phenotypic transition into fibroblast precursors in bone marrow-derived monocytes, marked by reduced CD45+ CD34- Col I+ and CD45+ CD11b+ Col I+ cell percentage. Our results indicate a potential mechanism by which the STAT3 inhibitor BP-1-102 inhibits bone marrow-derived monocyte transition into fibroblast precursors in an IL-33/STAT3-dependent manner and thereby alleviates renal interstitial fibrosis.

3.
Wei Sheng Yan Jiu ; 48(5): 774-779, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31601318

RESUMO

OBJECTIVE: To assess the prevalence of prenatal negative life events, and explore the effect of prenatal negative life events on pregnancy outcomes. METHODS: A total of 9137 postpartum women( average age: 28. 76±6. 53 years) who delivered live neonates with gestational age ≥28 weeks between April, 2012 to March, 2013 in 15 hospitals in Beijing, Guangdong, Hunan, Hubei, Sichuan and Shaanxi provinces were enrolled. Self-made questionnaire was used to collect general information, occurrence of negative life events during pregnancy, complications during pregnancy and pregnancy outcomes. Logistic regression models were used to analyze the effect of prenatal negative life events on adverse pregnancy outcomes and influencing factors of adverse pregnancy outcomes. RESULTS: In total of 1395 women( 15. 3%) had experienced prenatal negative life events, and 5439 women( 59. 5%) had adverse pregnancy outcomes. After adjusting for covariates, women who experienced prenatal negative life events had an increased risk of preterm birth( OR = 1. 257, 95% CI 1. 051-1. 504), and delivering low birth weight infants( OR = 1. 316, 95% CI 1. 055-1. 643). Multivariate Logistic regression models showed that prenatal negative life events( OR = 1. 201, 95% CI1. 056-1. 365), pregnancy-induced hypertension( OR = 2. 278, 95% CI 1. 867-2. 781), pre-pregnancy overweight or obese( OR = 1. 299, 95% CI 1. 140-1. 480) and delivery age above 35 years old( OR = 1. 197, 95% CI 1. 014-1. 413) were risk factors for adverse pregnancy outcomes; and primiparity( OR = 0. 808, 95% CI 0. 715-0. 913) were protective factors for adverse pregnancy outcomes. Among different types of negative life events, women with family disharmony had increased risk of adverse pregnancy outcomes than those without family disharmony after adjusting for covariates( OR = 1. 259, 95% CI1. 076-1. 473). CONCLUSION: In this study, prenatal negative life events were prevalent, and prenatal negative life events may increase the risk of pregnancy outcomes.

4.
BMJ Open ; 9(10): e028317, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601583

RESUMO

INTRODUCTION: Subjective cognitive decline (SCD) refers to individuals' perceived decline in memory and/or other cognitive abilities relative to their previous level of performance, while objective neuropsychological deficits are not observed. SCD may represent a preclinical phase of Alzheimer's disease. At this very early stage of decline, intervention could slow the rate of incipient decline to prolong and preserve cognitive and functional abilities. However, there is no effective treatment recommended for individuals with SCD. Acupuncture, as a non-pharmacological intervention, has been widely employed for patients with cognitive disorders. METHODS AND ANALYSIS: The proposed study is a randomised, assessor-blinded and placebo-controlled study that investigates the efficacy and mechanism of acupuncture in SCD. Sixty patients with SCD will be randomly allocated either into an acupuncture group or a sham acupuncture group. They will receive 24 sessions of real acupuncture treatment or identical treatment sessions using a placebo needle. Global cognitive changes based on a multidomain neuropsychological test battery will be evaluated to detect the clinical efficacy of acupuncture treatment at baseline and end of treatment. MRI scans will be used to explore acupuncture-related neuroplasticity changes. Correlation analyses will be performed to investigate the relationships between the changes in brain function and symptom improvement. ETHICS AND DISSEMINATION: The trial was approved by the research ethics committee. The results of the study will be published in a peer-reviewed academic journal and will also be disseminated electronically through conference presentations. TRIAL REGISTRATION NUMBER: NCT03444896.

5.
ACS Nano ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31594304

RESUMO

Proteins have been shown to be electrically-conductive if tethered to an electrode by means of a specific binding agent, allowing single molecules to be wired into an electrical sensing circuit. Such circuits allow enzymes to be used as as sensors, detectors and sequencing devices. We have engineered contact points into a Ф29 polymerase by introducing biotinylatable peptide sequences. The modified enzyme was bound to electrodes functionalized with streptavidin. Ф29 connected by one biotinylated contact and a second non-specific contact showed rapid small fluctuations in current when activated. Signals were greatly enhanced with two specific contacts. Features in the distributions of DC conductance increased by a factor 2 or more over the open- to closed conformational transition of the polymerase. Polymerase activity is manifested by rapid (millisecond) large (25% of background) current fluctuations imposed on the DC conductance.

6.
Genome Biol Evol ; 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31596462

RESUMO

Wolbachia are obligate intracellular bacteria which commonly infect various nematode and arthropod species. Genome sequences have been generated from arthropod samples following enrichment for the intracellular bacteria, and genomes have also been assembled from arthropod whole-genome sequencing projects. However, these methods remain challenging for infections that occur at low titers in hosts. Here we report the first Wolbachia genome assembled from host sequences using 10X Genomics linked-reads technology. The high read depth attainable by this method allows for recovery of intracellular bacteria that are at low concentrations. Based on the depth differences (714X for the insect and 59X for the bacterium), we assembled the genome of a Wolbachia in the parasitoid jewel wasp species Nasonia oneida. The final draft assembly consists of 1,293,406 bp in 47 scaffolds with 1,114 coding genes and 97.01% genome completeness assessed by checkM. Comparisons of the five Multi Locus Sequence Typing genes revealed that the sequenced Wolbachia genome is the A1 strain (henceforth wOneA1) previously reported in N. oneida. Pyrosequencing confirms that the wasp strain lacks A2 and B types previously detected in this insect, which were likely lost during laboratory culturing. Assembling bacterial genomes from host genome projects can provide an effective method for sequencing bacterial genomes, even when the infections occur at low density in sampled tissues.

7.
Drug Des Devel Ther ; 13: 3291-3306, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31571831

RESUMO

Objectives: This study was performed to evaluate the effects of muscone on the proliferation, migration and differentiation of human gingival mesenchymal stem cells (GMSCs) and to explore the relevant mechanisms. Materials and methods: We performed studies to determine the effects and mechanisms of muscone on GMSC proliferation, migration and differentiation. We conducted CCK-8, colony formation, transwell chamber, scratch wound, alkaline phosphatase (ALP) staining and activity, and alizarin red and oil red O staining assays, as well as real-time quantitative polymerase chain reaction (qRT-PCR), to ascertain the effects of muscone on GMSC proliferation, migration and differentiation in vitro. The mechanism by which muscone influences the osteogenic and adipogenic differentiation of GMSCs was elucidated by qRT-PCR and Western blotting. Results: We found that muscone significantly promoted GMSC proliferation, chemotaxis, wound healing and fat droplet formation and inhibited ALP activity and mineral deposition. Notably, we observed that the Wnt/ß-catenin pathway was closely related to the ability of muscone to inhibit the osteogenic differentiation and promote the adipogenic differentiation of GMSCs. The effect of muscone on the multidirectional differentiation capacity of GMSCs was significantly reversed by the agonist lithium chloride through the Wnt/ß-catenin signaling pathway. Conclusion: Muscone effectively increased the proliferation and migration, promoted the adipogenic differentiation and inhibited the osteogenic differentiation of GMSCs by inhibiting the Wnt/ß-catenin signaling pathway. These results may provide a theoretical basis for the application of GMSCs and muscone in tissue engineering and regenerative medicine.

8.
Nat Commun ; 10(1): 4405, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31562319

RESUMO

The emergence of smart electronics, human friendly robotics and supplemented or virtual reality demands electronic skins with both tactile and touchless perceptions for the manipulation of real and virtual objects. Here, we realize bifunctional electronic skins equipped with a compliant magnetic microelectromechanical system able to transduce both tactile-via mechanical pressure-and touchless-via magnetic fields-stimulations simultaneously. The magnetic microelectromechanical system separates electric signals from tactile and touchless interactions into two different regions, allowing the electronic skins to unambiguously distinguish the two modes in real time. Besides, its inherent magnetic specificity overcomes the interference from non-relevant objects and enables signal-programmable interactions. Ultimately, the magnetic microelectromechanical system enables complex interplay with physical objects enhanced with virtual content data in augmented reality, robotics, and medical applications.

9.
Neurosci Bull ; 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31502213

RESUMO

Scorpion venom heat-resistant peptide (SVHRP) is a component purified from Buthus martensii Karsch scorpion venom. Our previous studies have shown that SVHRP is neuroprotective in models of Alzheimer's disease and Parkinson's disease. The present study aimed to explore the potential neuroprotective effects of SVHRP on cerebral ischemia/reperfusion (I/R) injury, using a mouse model of middle cerebral artery occlusion/reperfusion (MCAO/R) and a cellular model of oxygen-glucose deprivation/reoxygenation (OGD/R). Our results showed that SVHRP treatment decreased the neurological deficit scores, edema formation, infarct volume and neuronal loss in the MCAO/R mice, and protected primary neurons against OGD/R insult. SVHRP pretreatment suppressed the alterations in protein levels of N-methyl-D-aspartate receptors (NMDARs) and phosphorylated p38 MAPK as well as some proinflammatory factors in both the animal and cellular models. These results suggest that SVHRP has neuroprotective effects against cerebral I/R injury, which might be associated with inhibition of the NMDA-MAPK-mediated excitotoxicity.

10.
Syst Biol Reprod Med ; : 1-9, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502483

RESUMO

Owing to less than 1% of motile spermatozoa in the ejaculated semen, severe asthenozoospermia is a serious threat to the male reproductive health. Herein, we identified a novel homozygous variant in CCDC9 (NC_000019.9: g.47763960C>T, NM_015603.3, NP_056418.1: p. Ser109Leu) in a patient from a consanguineous family. The variant was highly pathogenic and was predicted to be a candidate gene for asthenozoospermia through in silico analysis. The CCDC9 protein levels were significantly low and its morphology and ultrastructure were severely damaged in the spermatozoa containing the novel variant. Therefore, CCDC9 may be a novel pathogenic gene associated with severe asthenozoospermia. Abbreviations: CCDC9: coiled-coil domain containing 9; AZS: asthenozoospermia; MP: midpiece; MS: mitochondrial sheath; ODF: outer dense fiber; CP: central pair; DMT: doublet microtubule; IDA: inner dynein arm; ODA: outer dynein arm.

11.
Sci Total Environ ; 697: 133945, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31473551

RESUMO

The strength of methane (CH4) source of mangroves is not well understood, especially when including all CH4 pathways in consideration. This study measured CH4 fluxes by five pathways (sediments, pneumatophores, water surface, leaves, and stems) from four typical mangrove forests, including Kandelia candel without pneumatophores and three species with pneumatophores: Sonneratia apetala, Laguncularia racemosa and Bruguiera gymnorhiza-Bruguiera sexangula. The CH4 fluxes from sediments were 4.82±1.46mgCH4m-2h-1 for K. candel and 1.36±0.17mgCH4m-2h-1 for the other three with pneumatophores. Among the three communities with pneumatophores, S. apetala community had significantly greater emission rate than the other two (P<0.05). Pneumatophores in S. apetala were found to significantly decrease CH4 emission from sediments (P<0.01), while those in B. gymnorhiza-B. sexangula were significantly increase it (P<0.05). CH4 fluxes from waters were 3.48±1.11mgCH4m-2h-1, with the highest emission rate in the K. candel community for the duck farming. Leaves of mangroves except for those of K. candel were a weak CH4 daytime sink, but stems were a weak source. The total 72ha of mangroves in the Changning river basin emitted about 8.10Gg CH4 per year, with a weighted emission rate of about 1.29mgCH4m-2h-1. Our results suggested that mangroves are only a small methane source to atmosphere with great contribution from sediments and waters, only slight contribution from leaves and stems. Pneumatophores of different mangrove species played different roles in CH4 fluxes from sediments.

12.
Shanghai Kou Qiang Yi Xue ; 28(3): 317-320, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31489423

RESUMO

PURPOSE: To investigate the difference of radiological measurements between difficult-to-intubate obstructive sleep apnea hypopnea syndrome(OSAHS) patients and easily-to-intubate ones, and explore the feasibility of cephalometry in predicting difficult intubation. METHODS: Thirty male OSAS patients who received general anesthesia underwent cephalometrics before operation. Mallampati grade was used to evaluate the intubating condition of all patients before endotracheal intubation. After awake intubation and general anaesthesia, Cormack and Lehane laryngoscope grade was used to confirm the difficult-to-intubate patients and easily-to-intubate ones. The difficult-to-intubate patients were set into group I (n=13), the easily-to-intubate patients were set into group II (n=17). X-ray cephalometric radiographs were scanned into computer, 22 cephalometric parameters were measured with CASSOS 2001 cephalometric software. The measured items included hard tissue of maxilla and mandible, soft tissue of tongue and soft palate, airway space, hyoid and other parameters. SPSS13.0 software package was used for independent sample t test. RESULTS: The interface length of tongue and soft palate (CL), the tongue length (TGL) and the hyoid-mental distance (H-MP) were significantly different between the two groups (P<0.05). In difficult-to-intubate OSAS patients, the tongue area (TA) was larger, the soft palate length (PNS-U) was longer, the Atlanto-occipital gap (AOG) was shorter, but the difference was not significant. CONCLUTIONS: X-ray cephalometric indicators closest associated with difficult intubation in OSAHS patients are CL, TGL and H-MP, which are instructive for predicting difficult intubation.

13.
Nutrients ; 11(9)2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31480423

RESUMO

Although the major risk factors for liver cancer have been established, preventive factors for liver cancer have not been fully explored. We evaluated the association between raw garlic consumption and liver cancer in a large population-based case-control study in Eastern China. The study was conducted in Jiangsu, China, from 2003 to 2010. A total of 2011 incident liver cancer cases and 7933 randomly selected population-controls were interviewed. Epidemiological data including raw garlic intake and other exposures were collected, and serum markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection were assayed. Overall, eating raw garlic twice or more per week was inversely associated with liver cancer, with an adjusted odds ratio (aOR) of 0.77 (95% confidence interval (CI): 0.62-0.96) compared to those ingesting no raw garlic or less than twice per week. In stratified analyses, high intake of raw garlic was inversely associated with liver cancer among Hepatitis B surface antigen (HBsAg) negative individuals, frequent alcohol drinkers, those having history of eating mold-contaminated food or drinking raw water, and those without family history of liver cancer. Marginal interactions on an additive scale were observed between low raw garlic intake and HBsAg positivity (attributable proportion due to interaction (AP) = 0.31, 95% CI: -0.01-0.62) and heavy alcohol drinking (AP = 0.28, 95% CI: 0.00-0.57). Raw garlic consumption is inversely associated with liver cancer. Such an association shed some light on the potential etiologic role of garlic intake on liver cancer, which in turn might provide a possible dietary intervention to reduce liver cancer in Chinese population.

14.
PLoS One ; 14(9): e0222413, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31513649

RESUMO

The purpose of this study is to assess the antibiotics adjuvant effect of surfactin for boosting the treatment effect of amoxicillin. Surfactin is used as a surfactant to mediate flux of mono-and divalent cations, such as calcium, across lipid bilayer membranes. In this study, we demonstrated that surfactin can increase the activity of amoxicillin against avian pathogenic Escherichia coli (APEC) in vitro with antimicrobial assays such as minimum inhibitory concentrations (MIC) and fractional inhibitory concentration (FIC). Additionally in the model of chick infection, surfactin exerted adjuvant effects with amoxicillin against APEC by lowering the numerical value of mortality and liver bacterial loads, and regulating the expression of inflammatory cytokines et al. We concluded that surfactin can act as a novel antimicrobial adjuvant with amoxicillin against AEPC infection in chicken.

15.
BMJ Open ; 9(9): e028770, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31542742

RESUMO

OBJECTIVES: This study examines ethnic disparities in prevalence and patterns of smoking and nicotine dependence in rural southwest China. DESIGN: This was a cross-sectional design. SETTING: This study was conducted in rural Yunnan Province of China. PARTICIPANTS: 7027 consenting individuals aged ≥35 years among Han majority and four ethnic minority groups (Na Xi, Li Shu, Dai and Jing Po) participated in this study. Information about participants' demographic characteristics as well as smoking habits and an assessment of nicotine dependence with the Fagerstrom Test for Nicotine Dependence (FTND) was obtained using a standard questionnaire. RESULTS: Males had significantly higher prevalence of current smoking than females (64.8% and 44.4%, p<0.01). Among current smokers, the prevalence of nicotine dependence was significantly higher in males compared with females (19.9% and 7.1%, p<0.01). Jing Po men and women had the highest prevalence of current smokers (72.2% vs 23.1%, p<0.01), whereas the highest prevalence of nicotine dependence was found in male Dai current smokers and female Li Shu current smokers (44.8% vs 32.5%, p<0.01). Filtered cigarettes were the most popular form of tobacco used across all five ethnic groups. Over 75% of tobacco users initiated smoking and regularly smoked during adolescence, and those of minority ethnicity smoked regularly at a younger age than those of Han descent (p<0.05). Individuals in all five ethnic groups with higher levels of education had a lower probability of current smoking status (p<0.05), whereas a negative association of level of education with nicotine dependence was only observed in current smokers in the Han majority and Dai ethnic minority groups. Among Han majority current smokers, higher annual household income was associated with a higher risk of nicotine dependence (p<0.05). CONCLUSION: Future interventions to control tobacco use should be tailored to address ethnicity and socioeconomic factors.

16.
Chemosphere ; 235: 985-994, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561315

RESUMO

In this paper, the growth of S. bicolor in Cd-polluted sandy clay loam soil in north China, Cd accumulation in plant and the corresponding soil microbial community were characterized when the plant matured (140 d of growth). Cadmium promoted the growth of mature S. bicolor with higher height and heavier dry mass, especially at the spiked level of 1 mg kg-1 soil (P < 0.05). The higher microbial diversity was found under Cd stress at the spiked level of 15 mg kg-1, which basically corresponded with its influence on the plant growth. High-throughput sequencing data demonstrated that the predominant bacterial phyla include Proteobacteria (35.99% for Cd-polluted soil and 35.22% for the control soil), Chloroflexi (21.33% and 20.58%), Actinobacteria (12.00% and 12.89%), Acidobacteria (7.47% and 11.14%), Bacteroidetes (7.37% and 6.96%), Gemmatimonadetes (5.60% and 6.65%), Firmicutes (2.82% and 1.86%), Planctomycetes (2.47% and 0.95%), Saccharibacteria (1.26% and 1.11%). The predominant fungal phyla was Ascomycota, with the relative abundance of 89.96% for the control soil and 86.2% for the Cd-polluted soil. S. bicolor could grow well in sandy clay loam soil in northern China at low Cd lvel, but it could not accumulate cadmium at higher cadmium level. S. bicolor could be used for phytoextraction of cadmium from the lightly Cd-polluted soil.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31533123

RESUMO

BACKGROUND: Surgery is still the preferred treatment for tongue cancer. Reconstruction should be performed immediately after extensive resection of the tumor. The purpose of this study was to investigate the clinical effect, advantages, and disadvantages of radial forearm free (RFF) flap and anterolateral thigh (ALT) flap in tongue reconstruction after radical resection of tongue cancer. METHODS: Thirty-nine cases of tongue reconstruction with RFF flap or ALT flap from 2014 to 2018 were analyzed. The survival of the flap, the functional status after repair, and the influence on the donor area were examined, in addition to the advantages and disadvantages of the flap and the critical points of the technique. RESULTS: Twenty-one cases with RFF flaps and 18 cases with ALT flaps showed complete flap survival. Among them, 1 case involved a venous vessel crisis after an ALT operation, and the flap survived after reoperation after thrombus removal and anastomosis. The recovery of tongue function was as follows: 41.0% patients exhibited normal speech, 43.6% patients exhibited near-normal speech, 12.8% patients exhibited vague speech, and 2.6% patients could not speak. There was no significant difference between the 2 groups (p = 0.134). The recovery of tongue flexibility was as follows: 41.0% of the patients had normal postoperative tongue flexibility, 43.6% of the patients had slightly limited tongue flexibility, 12.8% of the patients had severely limited tongue flexibility, and 2.6% of the patients were completely limited. The difference between the 2 groups was statistically significant (p = 0.045). The postoperative diet of patients was as follows: 51.3% of patients had a regular diet, 33.3% of patients had soft foods, 12.8% of patients received a fluid diet, and 2.6% of patients could not eat after the operation. There was no significant difference between the 2 groups (p = 0.209). The satisfaction of donor area was as follows: 46.2% of the patients were satisfied with the donor area, 51.3% of the patients were basically satisfied with the donor area satisfaction, and 2.6% of the patients were not satisfied with the donor area satisfaction. There was no significant difference between the 2 groups (p = 0.809). CONCLUSION: The RFF flap is the most widely used technique in tongue reconstruction, especially in patients with tongue defects less than half of tongue tissue. However, for a large number of tissue defects caused by radical resection of advanced tongue cancer, the ALT flaps can provide a sufficient tissue volume, conceal scars after the operation, cause fewer complications in the donor area, and facilitate tongue function and aesthetic quality.

18.
Int J Biol Macromol ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31491513

RESUMO

MDG-1, a ß-D-fructan polysaccharide extracted from the roots of Ophiopogon japonicus, had preventive effect against obesity and hyperlipidemia in high-fat diet (HFD)-induced obesity mice. Interestingly, MDG-1, as an inulin-type fructan, is poorly absorbed and its possible mechanism against lipid disturbance remained unclear. The present study aimed to investigate the benefits of MDG-1 treatment on NAFLD model and elucidate mechanism from the perspective of gut-liver axis, especially about gut microbiota, short chain fatty acids (SCFAs) and hepatic lipid metabolism. In this study, after two months HFD feeding, C57BL/6J male mice were randomly divided into HFD group and various MDG-1 dose group. Results showed that MDG-1 markedly blocked weight gain, and ameliorated lipid accumulation, liver damage and macrovesicular steatosis. MDG-1 could restore gut microbiota balance and increase relative abundance of beneficial bacteria, especially SCFAs-producing bacteria. After degradation and utilization by the gut microbiota, MDG-1 could increase the contents of acetic acid and valeric acid, thus regulating inflammatory responses and hepatic lipid metabolism. Specifically, MDG-1 enhanced expression of hepatic phosphorylation of adenosine monophosphate-activated protein kinase, accompanying by regulating hepatic adipogenesis and adipocyte differentiation, thereby inhibiting progress of NAFLD. Our findings may provide new ways in the treatment of hyperlipidemia and lipid-related metabolic syndrome.

19.
Sci Total Environ ; 697: 134189, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31491640

RESUMO

By altering plant and soil properties and microclimate environments, grazing has a profound influence on the structure and function of grassland ecosystems. However, few studies have addressed the potential grazing effects on snow accumulation and subsequent spring soil water after snow melting and soil thawing. In this study, vegetation properties, snow accumulation and soil water were measured in experimental plots subjected to 8 years of cattle grazing comprising six different grazing intensity treatments in a typical temperate grassland in eastern Eurasia. The results indicated that heavy grazing reduced the snow depth by 51% and the snow mass by 40%. Snow accumulation first rapidly increased but then remained relatively stable with increases in both the aboveground biomass and canopy height. An obvious inflection point occurred at approximately 200 g m-2 aboveground biomass and at a 12.5 cm canopy height. The obvious difference in soil water content between the heavily grazed and ungrazed treatments occurred mainly in the spring after snow melting and soil thawing. The spring soil water content (0-30 cm) reached 31.5% in the ungrazed treatment (G0), which was 1.7 times that in the heavily grazed treatment (G0.92). The soil water content increased exponentially with increasing vegetation properties (aboveground biomass, canopy height and canopy cover), and a similar trend occurred with increasing snow mass and snow depth. Our structural equation modeling showed that both vegetation properties and snow accumulation had significant positive effects on spring soil water. By removing vegetation, grazing at increased intensities had significant, indirect suppressive effects on snow accumulation and spring soil water. Therefore, to obtain increased amounts of snow accumulation and spring soil water, land managers should consider reducing the grazing intensity or leaving some plots ungrazed.

20.
J Agric Food Chem ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31545895

RESUMO

Dry tea matrix contains an abundance of caffeine and polyphenols which are different from the food matrix (e.g., protein, lipid, and carbohydrates), and only a few studies have tried aflatoxins determination with tea samples. Here, a specific, accurate, and sensitive method was developed and validated for the simultaneous determination of aflatoxin B1, B2, G1, and G2 in dark teas. Aflatoxins were extracted by acetonitrile/water, press-filtered, and cleaned by multifunctional purification column (MFC) and immunoaffinity column (IAC) in tandem. The cleaned extract was analyzed by liquid chromatography tandem mass spectrometry. The matrix interference was effectively reduced by MFC-IAC cleaning method. Recoveries at the spiking concentrations of 5-60 µg/kg ranged from 77.5 to 93%, with relative standard deviations <11.0%. The correlation coefficients of aflatoxins standard were >0.998. The limits of detection were 0.024-0.21 µg/kg and the limits of quantification were 0.08-0.74 µg/kg. The intra- and interday accuracy ranged from 74 to 87%, and the intra- and interday precisions ranged from 0.4 to 3.1%. After the method validation, the aflatoxins contaminations in 100 collected dark teas were detected, and the results were compared with those of other methods.

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