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1.
JMIR Public Health Surveill ; 10: e47453, 2024 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-38315527

RESUMO

BACKGROUND: Cough is a common symptom during and after COVID-19 infection; however, few studies have described the cough profiles of COVID-19. OBJECTIVE: The aim of this study was to investigate the prevalence, severity, and associated risk factors of severe and persistent cough in individuals with COVID-19 during the latest wave of the Omicron variant in China. METHODS: In this nationwide cross-sectional study, we collected information of the characteristics of cough from individuals with infection of the SARS-CoV-2 Omicron variant using an online questionnaire sent between December 31, 2022, and January 11, 2023. RESULTS: There were 11,718 (n=7978, 68.1% female) nonhospitalized responders, with a median age of 37 (IQR 30-47) years who responded at a median of 16 (IQR 12-20) days from infection onset to the time of the survey. Cough was the most common symptom, occurring in 91.7% of participants, followed by fever, fatigue, and nasal congestion (68.8%-87.4%). The median cough visual analog scale (VAS) score was 70 (IQR 50-80) mm. Being female (odds ratio [OR] 1.31, 95% CI 1.20-1.43), having a COVID-19 vaccination history (OR 1.71, 95% CI 1.37-2.12), current smoking (OR 0.48, 95% CI 0.41-0.58), chronic cough (OR 2.04, 95% CI 1.69-2.45), coronary heart disease (OR 1.71, 95% CI 1.17-2.52), asthma (OR 1.22, 95% CI 1.02-1.46), and gastroesophageal reflux disease (GERD) (OR 1.21, 95% CI 1.01-1.45) were independent factors for severe cough (VAS>70, 37.4%). Among all respondents, 35.0% indicated having a productive cough, which was associated with risk factors of being female (OR 1.44, 95% CI 1.31-1.57), having asthma (OR 1.84, 95% CI 1.52-2.22), chronic cough (OR 1.44, 95% CI 1.19-1.74), and GERD (OR 1.22, 95% CI 1.01-1.47). Persistent cough (>3 weeks) occurred in 13.0% of individuals, which was associated with the risk factors of having diabetes (OR 2.24, 95% CI 1.30-3.85), asthma (OR 1.70, 95% CI 1.11-2.62), and chronic cough (OR 1.97, 95% CI 1.32-2.94). CONCLUSIONS: Cough is the most common symptom in nonhospitalized individuals with Omicron SARS-CoV-2 variant infection. Being female, having asthma, chronic cough, GERD, coronary heart disease, diabetes, and a COVID-19 vaccination history emerged as independent factors associated with severe cough, productive cough, and persistent cough.


Assuntos
Asma , COVID-19 , Doença das Coronárias , Diabetes Mellitus , Refluxo Gastroesofágico , Feminino , Humanos , Lactente , Masculino , SARS-CoV-2 , Estudos Transversais , Vacinas contra COVID-19 , COVID-19/complicações , COVID-19/epidemiologia , Tosse/epidemiologia , Fatores de Risco , China/epidemiologia , Asma/complicações , Asma/epidemiologia
2.
J Mater Chem B ; 12(5): 1232-1243, 2024 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-38165170

RESUMO

Cell therapy (also known as cell transplantation) has been considered promising as a next-generation living-cell therapy strategy to surpass the effects of traditional drugs. However, their practical clinical uses and product conversion are hampered by the unsatisfied viability and efficacy of the transplanted cells. Herein, we propose a synergistic enhancement strategy to address these issues by constructing 3D stem cell spheroids integrated with urchin-like hydroxyapatite microparticles (uHA). Specifically, cell-sized uHA microparticles were synthesized via a simple hydrothermal method using glutamic acid (Glu, E) as the co-template with good biocompatibility and structural antimicrobial performance (denoted as E-uHA). Combining with a hanging drop method, stem cell spheroids integrated with E-uHA were successfully obtained by culturing bone marrow mesenchymal stem cells (BMSCs) with a low concentration of the E-uHA suspensions (10 µg mL-1). The resulting composite spheroids of BMSCs/E-uHA deliver a high cellular viability, migration activity, and a superior osteogenic property compared to the 2D cultured counterpart or other BMSC spheroids. This work provides an effective strategy for integrating a secondary bio-functional component into stem cell spheroids for designing more cell therapy options with boosted cellular viability and therapeutic effect.


Assuntos
Micropartículas Derivadas de Células , Células-Tronco Mesenquimais , Osteogênese , Durapatita/farmacologia , Durapatita/química , Células-Tronco
3.
Mater Horiz ; 10(12): 5847-5858, 2023 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-37849349

RESUMO

Bio-based carbon fibers derived from lignin have gained significant attention due to their diverse and renewable sources, ease of extraction, and low cost. However, the current limitations of low specific surface area and insufficient electrical conductivity hinder the widespread application of lignin-derived carbon fibers (LCFs). In this work, highly conductive and porous LCFs are developed through melt-blowing, pretreatment, and carbonization processes. The effects of the carbonization temperature and heating rate on the structures and properties of the LCFs are systematically investigated. The resultant LCFs exhibit high electrical conductivity (71 400 S m-1) and a large specific surface area (923 m2 g-1). The assembled lithium-ion battery based on the LCF anodes demonstrates a long cycle life of >800 cycles and a high specific capacity of 466 mA h g-1. The findings of this study hold practical significance for promoting the utilization of lignin in the fields of energy storage, adsorption, and beyond.

4.
ACS Appl Mater Interfaces ; 15(28): 33480-33490, 2023 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-37394745

RESUMO

Artificial cell spheroids are gaining importance in tissue engineering and regenerative medicine fields. Biomimetic construction of stem cell spheroids is nevertheless challenging, and bioplatforms permitting controllable and high-efficient fabrication of functional stem cell spheroids are needed. Here, a fractal nanofiber-based bioplatform is developed based on a tunable interfacial-induced crystallization approach, allowing a programmed culture of artificial stem cell spheroids under an ultralow cell seeding density. Specifically, starting with the nanofibers of poly(L-lactide) (PLLA) and gelatin (PmGn), an interfacial growth of PLLA nanocrystals is subsequently performed to construct the fractal nanofiber-based biotemplates (C-PmGn). Cell experiments with human dental pulp stem cells (hDPSCs) demonstrate that the fractal C-PmGn could effectively decrease cell-matrix interactions, thus facilitating spontaneous cell spheroid formation even under a low cell seeding density (1 × 104 cells/cm2). Nanotopological properties of the C-PmGn bioplatform can be tuned by adjusting the fractal degree, thus enabling its suitability for the 3D culture of diverse hDPSC spheroids. Such a strategy provides a relatively simple and low-cost option for formation, expansion, and utility of stem cell spheroids. It offers another promising pathway to advance the development of stem cell therapies.


Assuntos
Nanofibras , Esferoides Celulares , Humanos , Nanofibras/química , Cristalização , Fractais , Células-Tronco
5.
BMC Psychol ; 11(1): 130, 2023 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-37098642

RESUMO

OBJECTIVES: This study aimed to explore the levels of hope in patients receiving maintenance hemodialysis (MHD), and whether anxiety and depression mediate the relationship between family function and hope. METHODS: The family APGAR index, hospital anxiety and depression scale, and Herth hope index were recorded using the self-reported questionnaires completed by 227 MHD patients. RESULTS: The family function can directly predict hope, positively predict hope through depression (ß = 0.052, p = 0.001), and positively predict hope through the chain mediating of anxiety and depression (ß = 0.087, p = 0.001), according to chain mediation analysis. The total effect size was 28.31%. The total indirect effect value was 0.139, and the total effect value was 0.491. CONCLUSIONS: Our findings suggest that family function had a direct impact on MHD patients' hope, and that lowering anxiety and depression can help to feel more hopeful.


Assuntos
Ansiedade , Depressão , Humanos , Estudos Transversais , Diálise Renal , Transtornos de Ansiedade , Inquéritos e Questionários
6.
Nanomaterials (Basel) ; 13(2)2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36678001

RESUMO

To develop membrane materials with good performance for water purification that are green and low cost, this work reports an organic-inorganic composite membrane composed of silk nanofibrils (SNFs) and palygorskite (PGS). To improve the stability of the the composite membrane, genipin was used as a crosslinking agent to induce the conformational transition of SNF chains from random coils to ß-sheets, reducing the swelling and hydrolysis of the membrane. The separation performance can be adjusted by tailoring the component ratio of the nanomaterial. The results showed that these membranes can effectively remove anionic dyes from water, and they exhibit excellent water permeability. The SNF-based membrane had strong mechanical and separation properties, and the PGS could tune the structure of composite membranes to enhance their permeability, so this green composite membrane has good prospects in water treatment and purification applications.

7.
Aust Crit Care ; 36(4): 515-520, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35610090

RESUMO

OBJECTIVE: The objectives of this study were to investigate paediatric nurses' knowledge, attitude, and practice (KAP) regarding the use of physical restraints and to explore the factors related to the use of physical restraints. Findings will provide a reference to develop standard procedures and training. BACKGROUND: Nurses' KAP regarding the use of physical restraints affect the use of physical restraints in the paediatric intensive care unit and neonatal intensive care unit. Understanding nurses' decision-making processes should inform strategies and methods for effectively reducing and regulating the use of physical restraints in paediatric patients in the intensive care unit (ICU) in China. METHODS: We conducted a cross-sectional survey of 823 registered ICU nurses from 12 children's hospitals in China between April and June, 2020. ICU nurses' KAP regarding the use of physical restraints in children were evaluated using a structured self-administered questionnaire that was distributed through an online platform. Descriptive and multiple linear regression analyses were used to examine the factors that influenced ICU nurses' KAP regarding the use of physical restraints in children. RESULTS: Overall, 49.8% of respondents were paediatric intensive care unit nurses, 25.0% of respondents were neonatal intensive care unit nurses, and 25.2% of respondents were other ICU nurses; 58.44% of nurses had received some training on the use of physical restraints in children. Mean total scores on the items addressing ICU nurses' knowledge (range, 0 [lowest level of knowledge] -11 [highest level of knowledge]), attitude (range, 11 [least likely to use physical restraint] - 55 [most likely to use physical restraint]), and practice (range, 14 [few skills] - 42 [good skills]) regarding the use of physical restraints in children were 8.00 ± 1.46, 30.67 ± 5.31, and 37.61 ± 3.46, respectively. Multiple linear regression analysis showed a higher level of education and less work experience (years) were related to higher knowledge scores; prior training in the use of physical restraint was related to lower attitude scores; and female, prior training in the use of physical restraints, and a higher level of education were related to higher practice scores. CONCLUSIONS: Nurses would like to use physical restraints without physician approval in an emergency or when they could not pay close attention to a child. There are a few standardised training and lack of clinical guidelines for paediatric nurses. We recommend establishing a standard of care for physical restraints in paediatric patients.


Assuntos
Atitude do Pessoal de Saúde , Enfermeiras e Enfermeiros , Recém-Nascido , Humanos , Criança , Feminino , Restrição Física , Estudos Transversais , Conhecimentos, Atitudes e Prática em Saúde , Competência Clínica , Unidades de Terapia Intensiva Neonatal , Cuidados Críticos , China
8.
Int J Biol Macromol ; 226: 646-659, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36521701

RESUMO

Lignin remains the second abundant source of renewable carbon with an aromatic structure. However, most of the lignin is burnt directly for power generation, with an effective utilization rate of <2 %, making value addition on lignin an urgent requirement. From this perspective, preparation of lignin-based carbon fibers has been widely studied as an effective way to increase value addition on lignin. However, lignin species are diverse and complex in structure, and the pathway that enables changes in lignin structure during pretreatment, fiber formation, stabilization, and carbonization is still uncertain. In this review, we condense the common structural evolution route from the previous studies, which can serve as a guide towards engineered lignin carbon fibers with high performance properties.


Assuntos
Carbono , Lignina , Lignina/química , Fibra de Carbono , Carbono/química , Oxirredução
9.
Sep Purif Technol ; 301: 122033, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36071792

RESUMO

The global outbreak and prevalence of coronavirus disease 2019 (COVID-19) has triggered an urgent demand for family hemodialysis equipment. It is particularly vital to design and apply superior adsorbents to adsorb toxins for reducing the usage of dialysate. In this work, hierarchically structural MgAl layered double oxides (LDO) with stretchable nanopores were exploited through a facile one-pot trisodium citrate (TSC) assistant hydrothermal reaction followed by calcination treatment for effectively adsorbing protein-bound uremic toxins such as hippuric acid (HA) or indoxyl sulfate (IS). The optimized MgAl LDO possessed flower-like spherical morphology, ultrahigh specific surface area (187.3 m2/g) and uniquely stretchable nanopores, which were more conducive to incorporating anions due to their unique memory effect endowing them with promising adsorption capacities for HA or IS. And the adsorption data could be better conformed to pseudo-second-order kinetic model and Langmuir isotherm determining that the maximum adsorption capacity of HA and IS was 129.8 mg/g and 63.1 mg/g, respectively. Furthermore, the computation of molecular size paired with the analysis of adsorption mechanism accurately revealed that high-efficiency toxin capture was mainly attributed to electrostatic interaction for internal intercalation and surface adsorption. Therefore, the application of such delicate LDO as new premium adsorbent would facilitate the development and popularization of family hemodialysis equipment.

10.
Int J Biol Macromol ; 216: 388-396, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35798080

RESUMO

Lignin is a biopolymer with high carbon content, making lignin-based carbon fiber an important research direction. In the process of carbonization to prepare carbon fibers, lignin fibers are easily softened and fused, which destroys the microstructure of fibers, thereby reducing the quality of lignin-based carbon fibers. Therefore, it is non-negligible to pre-oxidize lignin fibers before carbonization to prevent fiber fusion and maintain fiber structure. Therefore, the effects of pre-oxidation temperature and heating rate on the structure of pre-oxidation lignin fibers with controllable diameter and thickness prepared by melt-blowing were studied in detail. During pre-oxidation, crosslinking and aromatization of lignin fibers occurred, and alkyl and benzene rings were mainly oxidized to form carbonyl groups. The aromatization degree of the pre-oxidized product was recorded at 280 °C and 0.25 °C/min, and the oxygen content reached 15 %-20 %, making it suitable for the preparation of bio-based carbon fibers. On this basis, carbon fibers with porous morphology can be prepared with a graphitization of 0.54 and a resistivity of 0.02 Ω cm-1. These materials are expected to be applicable in sensors, catalytic materials and other fields.


Assuntos
Calefação , Lignina , Carbono/química , Fibra de Carbono , Lignina/química , Temperatura
11.
J Hazard Mater ; 424(Pt D): 127742, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34799164

RESUMO

Rapid and thorough removal of heavy metal ions in wastewater is critical for the urgent need of clean water. Herein, we prepared a high-performance thin film nanofibrous composite (TFNC) membrane consisting of a polyacrylonitrile (PAN)-UiO-66-(COOH)2 composite nanofibrous substrate (CPAN) and a calcium alginate (CaAlg) skin layer. Owing to abundant adsorption sites of UiO-66-(COOH)2 MOF, the optimal CPAN-2 nanofibrous substrate showed excellent adsorption capacity for lead ions. The maximum Pb2+ adsorption capacity of CPAN-2 substrate calculated by Langmuir isotherm model was 254.5 mg/g. Meanwhile, due to the relatively loose structure of CaAlg skin layer, this TFNC membrane showed high water permeate flux about 50 L m-2h-1 at 0.1 MPa, and the rejection for dyes was higher than 95%. Therefore, CaAlg/CPAN TFNC membranes were appropriate for dynamic adsorption/filtration to remove Pb2+. Compared with original CaAlg/PAN membrane, the optimal CaAlg/CPAN TFNC membrane showed much better ability to treat Pb(II)-containing wastewater and had good recyclability. Most importantly, the CaAlg/CPAN TFNC membrane could treat 7659 L m-2 wastewater containing single lead ions under WHO drinking water standard, and effectively deal with more simulated lead-containing wastewater. This work could provide a substitutable solution for effective removal of heavy metal ions and other various contaminants in wastewater.


Assuntos
Nanofibras , Poluentes Químicos da Água , Purificação da Água , Adsorção , Cinética , Chumbo , Estruturas Metalorgânicas , Ácidos Ftálicos , Poluentes Químicos da Água/análise
12.
World J Clin Cases ; 9(30): 9236-9243, 2021 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-34786410

RESUMO

BACKGROUND: Primary pulmonary enteric adenocarcinoma (PEAC) is a very rare subtype of invasive adenocarcinoma, and there have been no large studies on PEAC to date. Therefore, it is necessary to obtain much more information about the clinical and pathological features, diagnosis, differential diagnosis, and treatment of PEAC. CASE SUMMARY: All clinical data of six patients with confirmed PEAC from 2013 to 2018 were collected, and data on diagnosis, differential diagnosis, and treatment of PEAC are discussed combined with all the associated literature. The mean age of six patients was 64.0 ± 5.6 (59-73) years old. Their clinical manifestations were heterogeneous, and during their disease course, there were no gastrointestinal symptoms. There was no evidence from colonoscopy or imaging studies to suggest digestive tract tumors or new metastases. The most commonly mutated gene was KRAS (50.0%), and the pathological features of the six cases were similar to those of colorectal cancer. CDX2 (83.3%) and CK7 (66.7%) had the highest positive rates upon immunohistochemical examination. In the associated literature, 252 cases were identified, and the most commonly mutated gene was KRAS (42.9%). Additionally, CDX2 (68.3%) and CK7 (85.8%) had the highest positive rates. Patients mainly received surgery, chemotherapy, and radiotherapy, immunotherapy was not included. CONCLUSION: Positive results for CDX2 and CK7 play an important role in the diagnosis and differential diagnosis of PEAC, and immunotherapy or targeted therapy focused on KRAS needs to be further studied for the treatment of PEAC.

13.
PLoS One ; 16(4): e0250815, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33909683

RESUMO

BACKGROUND: COVID-19 is a respiratory infectious disease caused by SARS-CoV-2, and cardiovascular damage is commonly observed in affected patients. We sought to investigate the effect of SARS-CoV-2 infection on cardiac injury and hypertension during the current coronavirus pandemic. STUDY DESIGN AND METHODS: The clinical data of 366 hospitalized COVID-19-confirmed patients were analyzed. The clinical signs and laboratory findings were extracted from electronic medical records. Two independent, experienced clinicians reviewed and analyzed the data. RESULTS: Cardiac injury was found in 11.19% (30/268) of enrolled patients. 93.33% (28/30) of cardiac injury cases were in the severe group. The laboratory findings indicated that white blood cells, neutrophils, procalcitonin, C-reactive protein, lactate, and lactic dehydrogenase were positively associated with cardiac injury marker. Compared with healthy controls, the 190 patients without prior hypertension have higher AngⅡ level, of which 16 (8.42%) patients had a rise in blood pressure to the diagnostic criteria of hypertension during hospitalization, with a significantly increased level of the cTnI, procalcitonin, angiotensin-II (AngⅡ) than those normal blood pressure ones. Multivariate analysis indicated that elevated age, cTnI, the history of hypertension, and diabetes were independent predictors for illness severity. The predictive model, based on the four parameters and gender, has a good ability to identify the clinical severity of COVID-19 in hospitalized patients (area under the curve: 0.932, sensitivity: 98.67%, specificity: 75.68%). CONCLUSION: Hypertension, sometimes accompanied by elevated cTnI, may occur in COVID-19 patients and become a sequela. Enhancing Ang II signaling, driven by SARS-CoV-2 infection, might play an important role in the renin-angiotensin system, and consequently lead to the development of hypertension in COVID-19.


Assuntos
COVID-19/complicações , Traumatismos Cardíacos/epidemiologia , Hipertensão/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , COVID-19/metabolismo , COVID-19/fisiopatologia , Comorbidade , Progressão da Doença , Feminino , Traumatismos Cardíacos/virologia , Hospitalização , Humanos , Hipertensão/fisiopatologia , Hipertensão/virologia , Masculino , Registros Médicos , Pessoa de Meia-Idade , Pandemias , Sistema Renina-Angiotensina , SARS-CoV-2/patogenicidade
14.
J Thorac Dis ; 12(11): 6573-6585, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33282359

RESUMO

BACKGROUND: Evidence of treatment against cough variant asthma (CVA) is insufficient for the clinical practice in China. We aimed at evaluating the real-world effectiveness of montelukast (MONT) alone or in combination with low-dose inhaled corticosteroids (ICS) and low-dose ICS plus long-acting beta-2-agonists (LABA) for Chinese CVA patients in a multicentre, prospective, cohort study. METHODS: Adult patients diagnosed with CVA defined as chronic cough >8 weeks with a positive bronchial provocation test and normal chest X-ray findings were enrolled at respiratory clinics. Study treatment followed routine clinical practice. The investigators initiated MONT by 10 mg/day alone or in combination with a low-dose ICS +/- LABA and followed up treatment outcomes for 4 weeks. The primary outcome measure was the change in cough score (CS) from baseline. RESULTS: The study enrolled 247 patients (MONT =146, MONT + ICS =38, MONT + ICS/LABA =63). In the primary analysis, the mean change (95% CI) in CS at the end of the study was -1.2 (-1.6, -0.9), -0.9 (-1.5, -0.4), and -1.3 (-1.7, -0.8) in the three groups, respectively. MONT monotherapy had a satisfactory rate of weekly asthma control at the end of the study (83.5%, 95% CI: 75.1%, 89.4%) in the per-protocol analysis. Rates of weekly asthma control were similar in two MONT-based combination regimens (83.9%, 81.4%). Short-acting beta-2-agonist (SABA) user (≥2 times per week) was 16.8% in the MONT group. CONCLUSIONS: The real-world effectiveness of MONT alone or in combination with ICS or ICS and LABA was acceptable for CVA short-term control.

16.
Am J Infect Control ; 47(11): 1358-1364, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31277999

RESUMO

BACKGROUND: Few data are available on hospital-wide incidence of central line-associated bloodstream infection (CLABSI) rates in patients with central venous catheter (CVC) in China, where many systemic obstacles holding back evidence-based guidelines implementation exist. METHODS: This study was conducted prospectively in 2 phases. The baseline and intervention phases were performed in a teaching hospital in China, between January 2017 and October 2018. A systematic quality improvement (SQI) and multidisciplinary teamwork (MDT) CLABSI infection control program was introduced in the intervention phase. In the intensive care units (ICUs) and non-ICUs, CLABSIs were continuously monitored, data collected, then analyzed. RESULTS: After intervention, the CLABSI rate decreased from 2.84-0.56 per 1,000 CVC days in ICUs (P < .001), and from 0.82-0.47 per 1,000 CVC days in non-ICUs (P = .003). The length of time until CLABSI occurrence increased from 8.72-13.60 days in ICUs (P = .046), and from 10.00-12.00 days in non-ICUs (P = .048). The number of multidrug-resistant bacteria isolated from CLABSI episodes decreased both in ICUs and in non-ICUs. CONCLUSIONS: The SQI and MDT CLABSI infection control program is effective in reducing hospital-wide CLABSI in patients with CVC, both in ICUs and in non-ICUs.


Assuntos
Infecções Relacionadas a Cateter/prevenção & controle , Cateteres Venosos Centrais/efeitos adversos , Hospitais/normas , Controle de Infecções/organização & administração , Equipe de Assistência ao Paciente , Melhoria de Qualidade , Cateterismo Venoso Central , Equipamentos e Provisões Hospitalares , Higiene das Mãos , Administração Hospitalar , Humanos , Controle de Infecções/normas , Capacitação em Serviço , Unidades de Terapia Intensiva/normas , Política Organizacional , Embalagem de Produtos
17.
Exp Biol Med (Maywood) ; 244(9): 721-727, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31042074

RESUMO

IMPACT STATEMENT: Acute lung injury (ALI), a common complication of many serious health issues, such as serious infection, burns, and shock, is one of the most common critical illnesses in clinical practice with a high mortality rate of 30-40%. There are still short of effective prevention and treatment measures. Evidence is growing that hydrogen-rich saline (HRS) may be an effective drug for the prevention and treatment of ALI. However, the mechanisms involved in have not been clearly understood. In this study, we investigated the underling mechanisms by focusing on autophagy regulation. The results showed that HRS ameliorated lipopolysaccharide-induced ALI in mice by inhibiting autophagy over-activation through ROS/AMPK/mTOR pathway. HRS may be a new therapeutic strategy for ALI prevention and treatment in the future.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Lesão Pulmonar Aguda/etiologia , Autofagia , Hidrogênio/metabolismo , Lipopolissacarídeos/efeitos adversos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo , Lesão Pulmonar Aguda/metabolismo , Animais , Western Blotting , Líquido da Lavagem Broncoalveolar/citologia , Hidrogênio/administração & dosagem , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Solução Salina/metabolismo
18.
ACS Appl Mater Interfaces ; 10(49): 42891-42904, 2018 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-30427661

RESUMO

The emergence of Steiner minimal tree is of fundamental importance, and designing such geometric structure and developing its application have practical effect in material engineering and biomedicine. We used a cutting-edge nanotechnology, electrospinning/netting, to generate a Steiner geometrical poly(vinylidene fluoride) (PVDF) nanofiber/nanonet filter for removing airborne particulate matter (PM). Manipulation of surface morphologies by precise control of charged situation enabled the creation of two-dimensional nanonets with Steiner geometry. A significant crystalline phase transition of PVDF from α-phase to ß-phase was triggered by the dipole orientation and the intermolecular interactions derived from the electrostatic potential analysis. Particularly, the synergy of electrical interaction (ion-dipole and dipole-dipole) and hydrophobic interaction facilitated the formation of Steiner geometric structure during the evolution process of nanonets. The resultant PVDF nanofiber/nanonet air filter exhibited high filtration efficiency of 99.985% and low pressure drop of 66.7 Pa under the airflow velocity of 32 L/min for PM0.26 removal by the safest physical sieving mechanism. Furthermore, such filter possessed robust structure integrity for reusability, comparable optical transmittance, superior thermal stability, and prominent purification capacity for smoke PM2.5. The successful construction of such fascinating Steiner geometrical PVDF nanonets will provide new insights into the design and exploitation of novel filter media for air cleaning and haze treatment.

19.
J Food Sci ; 83(4): 1140-1148, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29577301

RESUMO

Chicken eggs are ingested by people after a series of processes, but to date, only a few studies have explored the nutrient variations caused by different heat treatments. In this work, the impacts of different heat treatments (4, 56, 65, and 100 °C on the in vitro digestibility of egg white proteins were investigated by hydrolysis with pepsin or pepsin + pancreatin to simulate human gastrointestinal digestion, and the digested products were identified using Nano-LC-ESI-MS/MS. Egg white proteins treated at 65 °C had the highest in vitro pepsin digestibility value whereas the pepsin + pancreatin digestibility increased significantly (P < 0.05) as the cooking temperature was raised. The molecular weight distribution of the digested products indicated that, when compared to pepsin-treated samples, pepsin + pancreatin-treated samples contained more low-molecular-weight peptides (m/z < 849.2) with higher signal intensities. The number of unique peptides identified in every digestion product showed a positive correlation with their in vitro digestibility. Additionally, bioactive peptides such as antioxidant, antimicrobial and antihypertensive peptides were found present in egg white digested products, especially in samples treated at 4 and 100 °C. PRACTICAL APPLICATION: These findings may facilitate a better understanding of nutritive values of egg white proteins and their digested products under different cooking temperatures, such as antibacterial and antioxidant peptides identified in the digestion samples treated, respectively at 4 and 100 °C. This study also provided information for improving the applications of eggs in the food industry as well as a theoretical basis for egg consumption.


Assuntos
Proteínas do Ovo/química , Temperatura Alta , Peptídeos/análise , Anti-Infecciosos/análise , Anti-Hipertensivos/análise , Antioxidantes/análise , Cromatografia Líquida , Culinária , Digestão , Humanos , Hidrólise , Peso Molecular , Valor Nutritivo , Pancreatina/metabolismo , Pepsina A/metabolismo , Espectrometria de Massas em Tandem
20.
Pathol Res Pract ; 212(10): 880-885, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27628667

RESUMO

The purpose of this study was to investigate the relation between expression of Wnt11, Rho-associated protein kinase 2 (Rock2), and its clinical characteristics in esophageal squamous cell carcinoma (ESCC). Expression of Wnt11 and Rock2 protein was examined by using immunohistochemistry that contained 260 paraffin-embedded specimens of ESCC and its adjacent normal tissues; expression of Wnt11 and Rock2 protein was verified by Western-blotting that contained 20 specimens of ESCC and its adjacent normal tissues. The positive rates of Wnt11 protein in normal esophageal epithelium tissue was 29.8% and in esophageal carcinomas tissue was 31.9%; there was no significant difference between the two groups(P>0.05); The positive rates of Rock2 protein in normal esophageal epithelium tissue was 12.3% and in esophageal carcinomas tissues was 56.5%, there was a significant difference between the two groups (p<0.05). The expression of Rock2 protein was significantly related with the invasion of vascular and there was no significantly difference between the expression of Rock2 protein and ESCC patients' tumor location, differentiation, T stage, and lymph node metastases. The abnormal expression of Rock2 protein may promote tumor cell invasion.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Neoplasias Esofágicas/metabolismo , Proteínas Wnt/metabolismo , Via de Sinalização Wnt/fisiologia , Quinases Associadas a rho/metabolismo , Carcinoma de Células Escamosas/patologia , Neoplasias Esofágicas/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Estadiamento de Neoplasias
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