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1.
Brain Stimul ; 13(1): 109-116, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31606448

RESUMO

BACKGROUND: Transcranial direct current stimulation (tDCS) has been explored in epilepsy with limited samples, varied parameters, and inconclusive results. We aimed to study the efficacy of tDCS for patients with refractory focal epilepsy. METHOD: We conducted a randomized, double-blind, sham-controlled, and three-arm (Group 1 (sham), Group 2 (20-min), and Group 3 (2 × 20-min)) tDCS parallel multicenter study. The primary outcome measurement was seizure frequencies (SFs). The study consisted of 28-days baseline, 14-days treatment, and 56-days follow-up. The cathode was placed over the epileptogenic focus, and the current intensity was 2 mA. The generalized estimating equations model, one-way analysis of variance, chi-square and Kruskal-Wallis test were used for analysis. RESULTS: Of the 82 enrolled patients, 70 patients were included for final analysis (Group 1, n = 21; Group 2, n = 24; and Group 3, n = 25). There was a significant reduction in SFs for both active tDCS groups compared with the sham group. Patients in Group 2 showed a significantly 50.73-21.91% greater reduction in SFs that lasted for 4 weeks (p = 0.008-0.060). Patients in Group 3 showed a significantly 63.19-49.79% greater reduction in SFs compared with the sham group that lasted for 5 weeks (p = 0.011-0.045). Patients in Group 3 had a 64.98-66.32% greater reduction in SFs at W9-W10, when compared with Group 2 (p = 0.021-0.022). CONCLUSION: Fourteen consecutive days tDCS significantly decreased SFs in patients with refractory focal epilepsy, with 2 × 20-min daily stimulation protocol being superior to 20-min daily stimulation protocol.

2.
Behav Brain Res ; 378: 112296, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31618623

RESUMO

Although activated microglia-induced neuroinflammation link to the physiopathology of major depressive disorder, the homeostasis of switchable M1/M2 microglia in treating depression are unclear. Recent accumulating evidences suggest that Sirtuin 1 (SIRT1), an NAD+-dependent deacetylase, plays a key role in mood regulation, yet its role in the polarization of microglia acting on depressive behaviors remains unknown. Here, we intended to investigate whether activation of SIRT1 in hippocampus has antidepressant potential in relation to microglial phenotypic switch. Chronic unpredictable mild stress (CUMS) treatment was performed on C57BL/6 mice, followed by injecting with SRT2104, a selective SIRT1 agonists. We found that activation of SIRT1 in hippocampus ameliorate CUMS-induced depressive-like behaviors, as indicated by sucrose preference test, tail suspension test and forced swim test. Moreover, activation of SIRT1 abrogated the increased expression of M1 markers (IL-6, IL-1ß and iNOS,) and decreased expression of M2 markers (IL-10, TGF-ß and Arignase1) induced by CUMS. Notably, activation of SIRT1 shifted microglia polarization toward the M2 phenotype in CUMS-induced depressive-like behaviors of mice. In addition, SRT2104 treatment ameliorated CUMS-induced SIRT1 decreased expression in the hippocampus coincides with the up-regulation phosphorylation levels of GSK3ß and PTEN. Taken together, these findings indicated that activation of SIRT1 ameliorate CUMS-induced depressive-like behaviors via shifting microglial polarization toward the M2 phenotype, thereby providing a novel and beneficial therapeutic approach for depression that may be translatable to depression patients in the future.

3.
Pac Symp Biocomput ; 25: 511-522, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31797623

RESUMO

Models have been proposed to extract temporal patterns from longitudinal electronic health records (EHR) for clinical predictive models. However, the common relations among patients (e.g., receiving the same medical treatments) were rarely considered. In this paper, we propose to learn patient similarity features as phenotypes from the aggregated patient-medical service matrix using non-negative matrix factorization. On real-world medical claim data, we show that the learned phenotypes are coherent within each group, and also explanatory and indicative of targeted diseases. We conducted experiments to predict the diagnoses for Chronic Lymphocytic Leukemia (CLL) patients. Results show that the phenotype-based similarity features can improve prediction over multiple baselines, including logistic regression, random forest, convolutional neural network, and more.

4.
Bioresour Technol ; 295: 122250, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31629281

RESUMO

Increasing nitrogenous contaminants have caused immense challenges to the environment and human health. As compared to physical and chemical methods, biological denitrification is considered to be an effective solution due to its environmental friendliness, high efficiency, and low cost. In the present work, a novel fungal strain identified as Fusarium solani (RADF-77) was isolated from cellulose material-supported denitrification reactor; this strain is capable of removing nitrogen under aerobic conditions. The average NO3--N removal rate for RADF-77 were 4.43 mg/(L·h) and 4.50 mg/(L·d), when using glucose and tea residue as carbon source, respectively. The nitrogen balance revealed that 53.66% of N vanished via gaseous products. Transcriptional results revealed that respiratory and assimilative nitrate reductases may work together for nitrate removal. Our results indicate that RADF-77 could be used as a potential means of enhancing nitrate-removal performance, as well as recycling tea residue, which is the main byproduct of the manufacture of tea extracts.


Assuntos
Desnitrificação , Fusarium , Aerobiose , Nitratos , Nitrificação , Nitrogênio
5.
Bioresour Technol ; 295: 122246, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31629286

RESUMO

Using biological treatment to deal with harmful algal blooms is highly potential over the physical and chemical methods due to its species specificity and eco-friendly characteristics. In this study, algicidal broth were produced from a Vibrio sp. co-culture composed mainly of V. brasilliensis and V. tubiashii. The productivity of the algicidal compounds was optimized under a dilution rate of 0.1 h-1 with a minimum algicidal broth dosage of 0.3% for 100% algal lysis. The algicidal threshold and EC50 of the spray-dried algicidal broth were 0.17 and 0.68 g/L, respectively. Treatment with the algicidal agents led to an increase in cellular reactive oxygen species (ROS) level that causes membrane damage as supported by the increase in Malondialdehyde (MDA) levels. and a further inhibition to the antioxidant system as indicated by a sharp decrease in the catalase (CAT) activity. The algicidal compound was identified as hexahydro pyrrolo[1,2-a] pyr azine-1,4-dione.


Assuntos
Dinoflagelados , Vibrio , Técnicas de Cocultura , Proliferação Nociva de Algas , Malondialdeído
6.
Addict Behav ; 101: 105976, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31101387

RESUMO

PURPOSE: The current study attempts to clarify the mediating role of cognitive function on the relationship between environmental pressure (pressure caused by negative parenting styles and dysfunctional school environments) and Internet Use Disorder (IUD); this study explores the effects of sex and left-behind children (LBC). METHODS: A cross-sectional sample of adolescents aged 12-15 years was recruited in 2018. A total of 3048 junior high school adolescents in rural areas of the Sichuan province in western China completed a series of psychological inventories, including the Adolescent Pathological Internet Use Scale (APIUS), the Junior High School Students' Perceived School Climate Inventory (PSCI-M), the Egna Minnen av. Barndoms Uppfostran (EMBU), and the Mental Health Screening Inventory for Children and Adolescents (MHS-C), for an analysis of IUD, school climate, parenting styles and cognition, respectively. RESULTS: Among the participants, 18.5% (N = 565) exhibited significant symptoms of IUD. The correlation analysis showed that IUD was positively correlated with parents' punishment, rejection and over-interference and academic pressure, whereas IUD was negatively associated with good teacher-student relationships, good schoolmate relationships and cognitive function scores. Structural equation modelling (SEM) showed that cognitive function partially contributed to the association between family and school pressures and IUD. DISCUSSION: Cognitive function is one of the mediating pathways through which environmental pressures may predict IUD among junior high school students. Interventions may target the mediating pathway of cognitive function to alleviate the negative impact of environmental pressure on IUD.

7.
Dev Comp Immunol ; 103: 103529, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31669309

RESUMO

The myeloid differentiation protein 2 (MD2)-related lipid-recognition (ML) proteins display diverse biological functions in host immunity and lipid metabolism by interacting with different lipids. Human MD2, an indispensable accessory protein in TLR4 signaling pathway, specifically recognizes lipopolysaccharides (LPS), thereby leading to the activation of TLR4 signaling pathway to produce many effectors that participate in inflammatory and immuneresponses against Gram-negative bacteria. Toll and immune deficiency (IMD) pathways are first characterized in Drosophila and are reportedly present in crustaceans, but the recognition and activation mechanism of these signaling pathways in crustaceans remains unclear. In the present study, a novel ML protein was characterized in mud crab (Scylla paramamosain) and designated as SpMD2. The complete SpMD2 cDNA sequence is 1114 bp long with a 465 bp open reading frame; it encodes a protein that contains 154 amino acids (aa). In the deduced protein, a signal peptide (1-21 aa residues) and a ML domain (43-151 aa residues) were predicted. SpMD2 shared a similar three-dimensional structure and a close evolutionary relationship with human MD2. SpMD2 was highly expressed in gills, hemocytes, intestine, and hepatopancreas and was upregulated in gills and hemocytes after challenges with bacteria, thereby suggesting its involvement in antibacterial defense. Western blot assay showed that SpMD2 possesses strong binding activities to different bacteria and two fungi. ELISA demonstrated that SpMD2 exhibits binding abilities to LPS, lipid A, peptidoglycan (PGN), and lipoteichoic acid (LTA). Its binding ability to LPS and lipid A were stronger than to PGN or LTA, implying that SpMD2 was an important LPS-binding protein in mud crab. Bacterial clearance assay revealed that the pre-incubation of Vibrio parahemolyticus with SpMD2 facilitates bacterial clearance in vivo and that knockdown of SpMD2 dramatically suppresses the bacterial clearance and decreases the expression of several antimicrobial peptides (AMPs). Furthermore, SpMD2 overexpression could enhance the promoter activity of SpALF2. These results revealed that SpMD2 affects bacterial clearance by regulating AMPs. Thus, by binding to LPS and by regulating AMPs, SpMD2 may function as a potential receptor, which is involved in the recognition and activation of a certain immune signaling pathway against Gram-negative bacteria. This study provides new insights into the diverse functions of ML proteins and into the antibacterial mechanisms of crustaceans.

8.
Carbohydr Polym ; 228: 115372, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635745

RESUMO

This study aimed at providing a novel approach for improving the physicochemical stability and solubility of algal oil nanocapsules through formation of electrostatic interactions and prebiotic carbohydrates systems composed of: octenyl-succinic anhydride (OSA) starch, OSA/inulin (IN), OSA/maltodextrin (MD), OSA/chitosan (CS), OSA/MD/IN, and OSA/CS/IN. IN, a functional prebiotic modifier, was found to significantly (p < 0.05) decrease emulsion viscosity and particle size, with OSA/CS/IN particles having significantly (p < 0.05) improved water solubility (4.96%) and wettability (749 s) compared to OSA/CS particles. Interestingly, OSA/CS/IN particles had the highest oxidative stability (three times that of bulk oil) and encapsulation efficiency (98.57%). OSA/CS/IN particles were also more hygroscopic than pure OSA particles. Furthermore, scanning electron microscopy (SEM) revealed OSA/CS/IN particles had less wrinkled, smoother surfaces, providing lower air permeability and better protection. Therefore, OSA/CS/IN, as a prebiotic encapsulation system, may lead to the value addition of algal oil.

9.
Sci Total Environ ; : 134669, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31796270

RESUMO

Eco-friendly treatment of refractory pollutants in wastewater is still full of challenge in catalytic oxidation and adsorption. In this study, based on the concept of green chemistry, sulfur-doped titanium dioxide hollow spheres modified by surfactant loaded on magnetic bentonite (CST/γ-Fe2O3-BT) is synthesized in two steps, and bisphenol A (BPA) was chosen as the representative organic pollutant. These materials were characterized by means of XRD, FTIR, SEM, EDS, TEM, XPS, BET, and VSM techniques. The adsorption and photodegradation behavior of CST/γ-Fe2O3-BT were examined. The Langmuir isotherm exhibited a better fit with a maximum adsorption capacity of 77.36 mg/g. At pH 7, the reaction rate constant (k) of the BPA photocatalytic degradation by product was 0.00104 min-1, and the adsorption equilibrium constant (K) was 0.04034L/mg. In addition, the composite can be recovered from the reaction mixture by applying an external magnetic field due to the existence of the superparamagnetic iron oxide nanoparticles in the construct. The recovered particles retained their catalytic activity which the catalytic activity of the material still reached 91% of the first catalytic experiment after 5 repetitive experiments. Results infer that the material has excellent reusability. Thus, CST/γ-Fe2O3-BT is a significant candidate for the treatment of recalcitrant organic pollutants in wastewater.

10.
Curr Mol Med ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31797757

RESUMO

BACKGROUND: Colorectal cancer (CRC) is the seconding leading cause of death of cancer worldwide, and distant metastasis is responsible for the poor prognosis in patients with advanced-stage CRC. RPS24 (ribosomal protein S24) as a ribosomal protein, multiple transcript variant encoding different isoforms have been found for this gene. Our previous studies have demonstrated that RPS24 is overexpressed in CRC. However, the mechanisms underlying the role of RPS24 in tumor development have not been fully defined. METHODS: Expression of RPS24 isoforms and lncRNA MVIH in CRC tissues and cell lines were quantified by real time PCR or western blotting assay. Endothelial tube formation assay was performed to determine the effect of RPS24 on tumor angiogenesis. The cell viability of HUVEC was determined by MTT assay, and the migration and invasion ability of HUVEC was detected by transwell assy. PGK1 secretion was tested with a specific ELISA kit. RESULTS: Here we found that RPS24c isoform was a major contributor to tumor angiogenesis, a vital process in tumor growth and metastasis. Real time PCR revealed that RPS24c isoform was high expressed in CRC tissues, while other isoforms are present in both normal and CRC tissues with no statistical difference. And change of RPS24 protein level is mainly due to fluctuation of RPS24c. Furthermore, we observed that silencing RPS24c could decrease angiogenesis by inhibiting tubule formation, HUVEC cell proliferation and migration. Additionally, we investigated the molecular mechanisms and demonstrated that RPS24c mRNA interacted with lncRNA MVIH, the binding-interaction enhanced the stability of each other, thereby activated angiogenesis by inhibiting the secretion of PGK1. CONCLUSION: RPS24c facilitates tumor angiogenesis via the RPS24c/MVIH/PGK1 pathway in CRC. RPS24c inhibition may be a novel option for anti-vascular treatment in CRC.

11.
Cell Mol Biol Lett ; 24: 62, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31798643

RESUMO

Background: Myocardial ischaemia reperfusion injury (MIRI) is a difficult problem in clinical practice, and it may involve various microRNAs. This study investigated the role that endogenous microRNA-146a plays in myocardial ischaemia reperfusion and explored the possible target genes. Methods: MIRI models were established in microRNA-146a deficient (KO) and wild type (WT) mice. MicroRNA-146a expression was evaluated in the myocardium of WT mice after reperfusion. The heart function, area of myocardium infarction and in situ apoptosis were compared between the KO and WT mice. Microarray was used to explore possible target genes of microRNA-146a, while qRT-PCR and dual luciferase reporter assays were used for verification. Western blotting was performed to detect the expression levels of the target gene and related signalling molecules. A rescue study was used for further testing. Results: MicroRNA-146a was upregulated 1 h after reperfusion. MicroRNA-146a deficiency decreased heart function and increased myocardial infarction and apoptosis. Microarray detected 19 apoptosis genes upregulated in the KO mice compared with the WT mice. qRT-PCR and dual luciferase verified that Med1 was one target gene of microRNA-146a. TRAP220, encoded by Med1 in the KO mice, was upregulated, accompanied by an amplified ratio of Bax/Bcl2 and increased cleaved caspase-3. Inhibition of microRNA-146a in H9C2 cells caused increased TRAP220 expression and more apoptosis under the stimulus of hypoxia and re-oxygenation, while knockdown of the increased TRAP220 expression led to decreased cell apoptosis. Conclusions: MicroRNA-146a exerts a protective effect against MIRI, which might be partially mediated by the target gene Med1 and related to the apoptosis signalling pathway.

12.
Adv Mater ; : e1904106, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31799752

RESUMO

Bacterial infection is one of the top ten leading causes of death globally and the worst killer in low-income countries. The overuse of antibiotics leads to ever-increasing antibiotic resistance, posing a severe threat to human health. Recent advances in nanotechnology provide new opportunities to address the challenges in bacterial infection by killing germs without using antibiotics. Antibiotic-free antibacterial strategies enabled by advanced nanomaterials are presented. Nanomaterials are classified on the basis of their mode of action: nanomaterials with intrinsic or light-mediated bactericidal properties and others that serve as vehicles for the delivery of natural antibacterial compounds. Specific attention is given to antibacterial mechanisms and the structure-performance relationship. Practical antibacterial applications employing these antibiotic-free strategies are also introduced. Current challenges in this field and future perspectives are presented to stimulate new technologies and their translation to fight against bacterial infection.

13.
Water Sci Technol ; 80(6): 1031-1041, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31799947

RESUMO

Being a fundamental issue regarding sewage treatment, heavy metals removal from industrial effluents has been subject to intense scrutiny in both the academic and practical worlds. The removal of pentavalent arsenic (As(V)), one of the most poisonous pollutants, was investigated using a sodium persulfate and iron powder system activated by ferrous ions (Fe2+-ZVI-PS). As(V) could be effectively removed by an Fe2+-ZVI-PS system in a timely fashion (minute scale) with high removal rates (more than 90.0%) over a wide range of pH (1-9) and concentration (20-100 mg/L). The removal of As(V) by the Fe2+-ZVI-PS system integrated favorably with the pseudo-second-order reaction kinetics. Researches on X-ray photoelectron spectroscopy (XPS) demonstrated that the Fe2+-ZVI-PS system enables the removal of As(V) through the process of co-precipitation and adsorption. Our findings thus emphasized that the Fe2+-ZVI-PS system should be an effective trigger to purifying arsenic from the environment. Our results indicated that the Fe2+-ZVI-PS system could be an effective candidate for remediation of arsenic in the environment.

14.
J BUON ; 24(5): 1852-1860, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31786847

RESUMO

PURPOSE: This study was designed to compare the survival and surgical outcomes of laparoscopic colectomy (LC) in elderly and non-elderly patients with transverse colon cancer (TCC). METHODS: From January 2011 to January 2018, 44 elderly (aged ≥70 years) and 72 non-elderly (aged <70 years) patients with TCC underwent LC at our institution. The survival and surgical outcomes of the two groups were compared retrospectively. RESULTS: Preoperatively, the Charlson Comorbidity Index (CCI) and American Society of Anesthesiologists score were higher in the elderly group than in the non-elderly group. There were no significant differences between the groups in operating time, intraoperative blood loss, conversion rate, pathologic data, 30-day postoperative mortality rate, incidence of 30-day postoperative complications, incidence of major complications, or compliance with adjuvant chemotherapy. During the follow-up period, differences in recurrence rate, 5-year overall survival (OS) rate, and 5-year disease-free survival (DFS) rate between the groups were not significant. CONCLUSION: Although elderly patients with TCC have higher surgical risk than non-elderly patients, performing LC in elderly patients is safe and effective. The survival and surgical outcomes in elderly patients were similar to those in non-elderly patients.

15.
J Hazard Mater ; : 121684, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31784128

RESUMO

Microbial degradation is considered to be the most acceptable method for degradation of chlorimuron-ethyl, a typical long-term residual sulfonylurea herbicide, but the underlying mechanism at the genetic and biochemical levels is unclear. In this work, the genome sequence of the chlorimuron-ethyl-degrading bacterium Rhodococcus erythropolis D310-1 was completed, and the gene clusters responsible for the degradation of chlorimuron-ethyl in D310-1 were predicted. A carboxylesterase gene, carE, suggested to be responsible for carboxylesterase de-esterification, was cloned from D310-1. CarE was expressed in Escherichia coli BL21 and purified to homogeneity. The active site of the chlorimuron-ethyl-degrading enzyme CarE and the biochemical activities of CarE were elucidated. The results demonstrated that CarE is involved in catalyzing the de-esterification of chlorimuron-ethyl. A carE deletion mutant strain, D310-1ΔcarE, was constructed, and the chlorimuron-ethyl degradation rate in the presence of 100 mg L-1 chlorimuron-ethyl within 120 h decreased from 86.5 % (wild-type strain D310-1) to 58.2 % (mutant strain D310-1ΔcarE). Introduction of the plasmid pNit-carE restored the ability of the mutant strain to utilize chlorimuron-ethyl. This study is the first to demonstrate that carboxylesterase can catalyze the de-esterification reaction of chlorimuron-ethyl and provides new insights into the mechanism underlying the degradation of sulfonylurea herbicides and a theoretical basis for the utilization of enzyme resources.

16.
Histol Histopathol ; : 18188, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793657

RESUMO

Accumulating dysregulated lncRNAs have been demonstrated to execute vital functions in the pathogenesis and progress of gastric cancer (GC) through versatile molecular mechanisms. In this review, we classify the mechanisms of dysregulated lncRNAs in GC into several governing types according to their roles at molecular level. For each regulatory role, we illustrate several instructive examples and introduce significant effects of lncRNAs on cellular biological properties of GC. Besides, we summarize a group of lncRNA-signatures that are potential biomarkers in the prediction of prognosis for GC patients.

17.
BMC Med Inform Decis Mak ; 19(Suppl 5): 238, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31801534

RESUMO

BACKGROUND: Accurately recognizing rare diseases based on symptom description is an important task in patient triage, early risk stratification, and target therapies. However, due to the very nature of rare diseases, the lack of historical data poses a great challenge to machine learning-based approaches. On the other hand, medical knowledge in automatically constructed knowledge graphs (KGs) has the potential to compensate the lack of labeled training examples. This work aims to develop a rare disease classification algorithm that makes effective use of a knowledge graph, even when the graph is imperfect. METHOD: We develop a text classification algorithm that represents a document as a combination of a "bag of words" and a "bag of knowledge terms," where a "knowledge term" is a term shared between the document and the subgraph of KG relevant to the disease classification task. We use two Chinese disease diagnosis corpora to evaluate the algorithm. The first one, HaoDaiFu, contains 51,374 chief complaints categorized into 805 diseases. The second data set, ChinaRe, contains 86,663 patient descriptions categorized into 44 disease categories. RESULTS: On the two evaluation data sets, the proposed algorithm delivers robust performance and outperforms a wide range of baselines, including resampling, deep learning, and feature selection approaches. Both classification-based metric (macro-averaged F1 score) and ranking-based metric (mean reciprocal rank) are used in evaluation. CONCLUSION: Medical knowledge in large-scale knowledge graphs can be effectively leveraged to improve rare diseases classification models, even when the knowledge graph is incomplete.

18.
World J Gastroenterol ; 25(44): 6508-6526, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31802831

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) are aberrant and play critical roles in gastric cancer (GC) progression and metastasis. Searching for coexpressed lncRNA clusters or representative biomarkers related to malignant phenotypes of GC may help to elucidate the mechanism of tumor development and predict the prognosis of GC. AIM: To investigate the prognostic value of NOTCH1 associated with lncRNA in T cell acute lymphoblastic leukemia 1 (NALT1) in GC and the mechanism of its involvement in GC invasion and metastasis. METHODS: RNA sequencing and corresponding clinical data were downloaded from The Cancer Genome Atlas database. The significance module was studied by weighted gene coexpression network analysis. A total of 336 clinical samples were included in the study. Gene silencing, reverse transcription polymerase chain reaction, western blotting, scrape motility assay, and Transwell migration assay were used to assess the function of hub-lncRNAs. RESULTS: At the transcriptome level, 3339 differentially expressed lncRNAs were obtained. weighted gene coexpression network analysis was used to obtain 15 lncRNA clusters and observe their coexpression. Pearson's correlation showed that blue module was correlated with tumor grade and survival. NALT1 was the hub-lncRNA of blue module and was an independent risk factor for GC prognosis. NALT1 was overexpressed in GC and its expression was closely related to invasion and metastasis. The mechanism may involve NALT1 regulation of NOTCH1, which is associated with lncRNA in T cell acute lymphoblastic leukemia, through cis regulation, thereby affecting the expression of the NOTCH signaling pathway. CONCLUSION: NALT1 is overexpressed and promotes invasion and metastasis of GC. The mechanism may be related to regulation of NOTCH1 by NALT1 and its effect on NOTCH signaling pathway expression.

19.
Chem Asian J ; 14(23): 4352-4357, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31777169

RESUMO

A maleimide-based acyclic enediyne with salicylaldiminato substituents at the alkyne termini was synthesized, which was further chelated with three kinds of metal-ions, CuII , ZnII , and MgII , and form metalloenediynes. The cycloaromatization of this thermally inactive enediyne ligand was greatly accelerated through the coordination with metal ions. Specifically, the CuII -metalloenediyne showed an extremely low onset temperature of 55 °C and underwent spontaneous cycloaromatization at ambient temperature to produce free radicals, followed by generation of reactive oxygen species in the physiological environment. The metalloenediyne exhibited excellent DNA cleavage ability and high cytotoxicity towards HeLa cells, with half-maximal inhibitory concentration values comparable to many commercial antitumor agents. The combination of the electron-withdrawing effect of the maleimide moiety at the ene position and metal coordination at the yne termini provides a new inspiration for designing and synthesizing highly efficient enediyne antitumor agents.

20.
Surg Endosc ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686203

RESUMO

OBJECTIVE: Middle pancreatectomy (MP) is safe and feasible in patients with benign or low-grade malignant tumors located at the neck or proximal body of the pancreas. As a tissue-sparing operation, MP can preserve normal pancreatic function and reduce the risk of postoperative endocrine and exocrine insufficiency. However, the morbidity, especially the postoperative pancreatic fistula (POPF) rate, remains high. A robot-assisted surgical system may provide patients with less trauma; however, there are few reports on robot-assisted middle pancreatectomy (RMP). We describe the experience of RMP at our center to illustrate the learning curve (LC). METHODS: From August 2010 to July 2017, 100 patients underwent RMP in the Pancreatic Disease Center of Shanghai Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine. Patient characteristics, operative outcomes, and oncological outcomes were collected and analyzed. The CUSUM curve was analyzed according to operative time and estimated blood loss (EBL) and was used to describe the LC and identify the flexion points. RESULTS: Among the 100 patients who underwent RMP in our hospital, the mean age was 47.5 ± 14.2 years, and 69 patients were female. From the CUSUM curve, we found two flexion points: cases 12 and 44. After 44 cases, the rate of improvement was much faster. We separated the patients into two groups based on the LC (cases 1-44 and cases 45-100). There were significant improvements in operative time (173.1 ± 44.7 min vs. 137.3 ± 30.1 min, p < 0.001) and EBL (103.4 ± 90.0 ml vs. 69.3 ± 53.9 ml, p = 0.021). The overall POPF rate was 32% (32/100), while the incidence rate of biochemical leakage was 14% (14/100). However, there was no significant difference in the risk of POPF or other complications between the two groups. The postoperative length of stay (LOS) was also not different. The 90-day mortality rate was 1%. From our long-term follow-up, pancreatic function was preserved in most patients, with only three cases of endocrine insufficiency and two cases of exocrine insufficiency. CONCLUSION: RMP was helpful and a good choice for the selected patients. PF was the main complication and has not been improved until now. There were two flexion points in the LC at cases 12 and 44. More cases are needed to gain more experience. A larger sample size and prospective studies are needed to verify the advantage of RMP.

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