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1.
ACS Nano ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31670942

RESUMO

Photodynamic therapy (PDT) is a clinically approved, minimally invasive therapeutic technique that can induce the regression of targeted lesions via generating excess cytotoxic reactive oxygen species. However, due to the limited penetration depth of visible excitation light and the intrinsic hypoxia microenvironment of solid tumors, the efficacy of PDT in the treatment of cancer, especially deep-seated or large tumors, is unsatisfactory. Herein, we developed an efficient in vivo PDT system based on a nanomaterial, dihydrolipoic acid coated gold nanocluster (AuNC@DHLA), that combined the advantages of large penetration depth in tissue, extremely high two-photon (TP) absorption cross section (σ2 ∼ 106 GM), efficient ROS generation, a type I photochemical mechanism, and negligible in vivo toxicity. With AuNC@DHLA as the photosensitizer, highly efficient in vivo TP-PDT has been achieved.

2.
Environ Sci Technol ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31689090

RESUMO

Rejection of endocrine-disrupting compounds (EDCs) by thin film composite (TFC) polyamide membranes remains to be a challenging issue in wastewater reclamation applications due to the unfavourable hydrophobic interaction between EDCs and membrane. Herein, we investigated the incorporation of hydrophilic metal organic frameworks (MOFs) into polyamide layer to create water/EDCs selective nanochannels for enhancing EDCs rejection. Using MIL-101(Cr) MOF as a nanofiller, the water flux of MOF0.20 TFC membrane (0.20 wt/v % MOF in n-hexane) was 2.3 times of that of the control. The rejection rates against EDCs involving methylparaben, propylparaben, benzylparaben and bisphenol A (BPA) by MOF0.20 were also significantly higher than the respective values of the control membrane, with the water/EDC selectivity (e.g., A/BBPA) of MOF0.20 approximately doubled compared to that of the control. Further single salt rejection and gold nanoparticle filtration tests confirmed that the hydrophilic nanochannels created by MOFs played a critical role in membrane transport, accounting for the significant enhancement of EDCs rejection of the modified TFC membrane. This study demonstrates a promising membrane modification protocol using hydrophilic MOFs for achieving selective removal of EDCs and high-efficient wastewater reclamation using TFC membranes.

3.
Environ Res ; : 108861, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31703975

RESUMO

An electrochemical dynamic membrane filtration (EDMF) system for simultaneous solid-liquid separation (also protecting electrodes against fouling) and sewage disinfection was developed. At a low voltage of 2.5 V, efficient disinfection performance was achieved in the EDMF, with ~100% log removal efficiency (no detectable bacteria in the effluent). Results also demonstrated that the EDMF system, operated at membrane flux of 100 L/(m2 h), could maintain long-lasting bacterial disinfection efficiency of real wastewater (~100% log removal) in continuous flow tests. Transmembrane pressure (TMP) increased from 0.8 kPa to 22 kPa within 80 d (one operation cycle), and cleaning of EDMF could effectively restore TMP and biocidal behaviors for subsequent filtration cycles. In contrast, without dynamic membrane, the disinfection efficiency was decreased from initial ~100% log removal (with no detectable live bacteria) to ~44.4% log removal within 7 d. Reactive oxygen species (ROS)-mediated oxidation was responsible for bacteria disinfection in the EDMF, and HO• and H2O2 generated in this system played a dominant role, causing damage to cell membranes and K+ leakage from cytosol. Moreover, catalase and superoxide dismutase for intracellular ROS attenuation were inhibited, resulting in the increase of intracellular oxidative stress and thus high-efficient disinfection. These results highlight the potential of EDMF system to be used for wastewater treatment and disinfection.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31664615

RESUMO

It is well-known that curcumin, as a plant substance, has vascular protective effects. TRPV4 (transient receptor potential vanilloid 4) is a highly Ca2+-selective channel in vascular endothelium. In our study, fluorescent Ca2+ imaging in mesenteric arterial endothelial cells (MAECs) and overexpressed TRPV4 human embryonic kidney (HEK293) cells showed that curcumin dose-dependently stimulated Ca2+ influx. Whole-cell patch clamp proved that curcumin stimulated the TRPV4-mediated currents in TRPV4-HEK293 cells. The TRPV4-specific blocker HC067047 markedly decreased the whole-cell current. Molecular modeling and docking showed that the binding site of curcumin and TRPV4 was mainly in the amino acid sequence LYS340-LEU349 of TRPV4 protein. Furthermore, curcumin dose-dependently induced the endothelium-dependent vessel dilatation in small mesenteric arteries. Therefore, our results demonstrated that curcumin stimulates Ca2+ entry in endothelial cells and improves endothelium-dependent vessel relaxation by activating TRPV4 channels. Moreover, we identified the specific binding sites of curcumin and TRPV4, thereby highlighting its potential therapeutic target of cardiovascular diseases.

5.
Environ Pollut ; 255(Pt 2): 113299, 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31585405

RESUMO

Lipophilic marine algal toxins (LMATs) pose a potential threat to the health of marine shellfish consumers and marine breeding industries. In this study, LMATs in dissolved phases (DP) and particulate phases (PP) in the seawater of Jiaozhou Bay were accurately determined over four seasons to understand their composition, level, phase partitioning, spatiotemporal variation, and potential sources in aquatic environments of a typical semi-closed mariculture bay. Various LMATs, such as okadaic acid (OA), dinophysistoxin-1 (DTX1), dinophysistoxin-2 (DTX2), gymnodimine (GYM), 13-desmethyl spirolide C (SPX1), pectenotoxin-2 (PTX2), pectenotoxin-2 seco acid (PTX2 SA), and pectenotoxin-11 (PTX11), were detected in DP and PP; of these, OA and PTX2 were the dominant LMATs in DP and PP, respectively. The average proportion of ΣLMATs in DP (97.5%) was significantly higher than that in PP (2.5%), which indicates that LMATs are predominantly partitioned into DP. The total concentrations of LMATs in DP ranged from 4.16 ng/L to 23.19 ng/L (mean, 13.35 ng/L) over four seasons. The highest levels of LMATs in DP and PP were found in summer (mean, 16.71 ng/L) and spring, respectively, while the maximum variety of LMATs was found in autumn. This result suggests that seasonal changes could influence the composition, concentration, and phase partitioning of LMATs in aquatic environments of a coastal semi-closed mariculture bay. ΣLMAT concentrations were higher in the western region than in the eastern region of the bay, where shellfish may have a greater risk of exposure. Dinophysis acuminata, Dinophysis fortii, and Prorocentrum minimum were the potential sources of LMATs in the aquaculture seawater. Overall, various LMATs occurred in the semi-closed mariculture bay, and the persistence and bioavailability of these toxins in aquaculture seawater should be determined in future research.

6.
Bioresour Technol ; 295: 122241, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31627067

RESUMO

An anoxic-aerobic membrane bioreactor (AO-MBR), an anaerobic side-stream reactor (ASSR) coupled MBR (A-MBR), and an MBR with ASSR packed with carriers (AP-MBR) were operated parallelly to investigate biological nutrient removal, microbial community structure and mass balance of nutrients in sludge reduction systems. Compared to AO-MBR, A-MBR and AP-MBR were both efficient in COD and NH4+-N removal, had significantly higher nitrogen removal, reduced sludge production by 35.0% and 45.9%, but deteriorated biological phosphorus removal. Nitrosomonadaceae and Nitrospira were major bacteria responsible for ammonium and nitrite oxidation in the three systems. Inserting ASSR and packing carriers both favored denitrifying bacteria enrichment and organic substances release, and thus resulted in higher nitrate uptake rate (NUR) in the anoxic tank. Higher endogenous NUR in ASSR than in anoxic tank also indicated that ASSR and carriers both accelerated sludge decay. Denitrification and sludge reduction occurred in ASSR played important roles in biological nutrient removal.

7.
Med Image Anal ; 59: 101565, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31630010

RESUMO

The availability of a large amount of annotated data is critical for many medical image analysis applications, in particular for those relying on deep learning methods which are known to be data-hungry. However, annotated medical data, especially multimodal data, is often scarce and costly to obtain. In this paper, we address the problem of synthesizing multi-parameter magnetic resonance imaging data (i.e. mp-MRI), which typically consists of Apparent Diffusion Coefficient (ADC) and T2-weighted (T2w) images, containing clinically significant (CS) prostate cancer (PCa) via semi-supervised learning and adversarial learning. Specifically, our synthesizer generates mp-MRI data in a sequential manner: first utilizing a decoder to generate an ADC map from a 128-d latent vector, followed by translating the ADC to the T2w image via U-Net. The synthesizer is trained in a semi-supervised manner. In the supervised training process, a limited amount of paired ADC-T2w images and the corresponding ADC encodings are provided and the synthesizer learns the paired relationship by explicitly minimizing the reconstruction losses between synthetic and real images. To avoid overfitting limited ADC encodings, an unlimited amount of random latent vectors and unpaired ADC-T2w Images are utilized in the unsupervised training process for learning the marginal image distributions of real images. To improve the robustness for training the synthesizer, we decompose the difficult task of generating full-size images into several simpler tasks which generate sub-images only. A StitchLayer is then employed to seamlessly fuse sub-images together in an interlaced manner into a full-size image. In addition, to enforce the synthetic images to indeed contain distinguishable CS PCa lesions, we propose to also maximize an auxiliary distance of Jensen-Shannon divergence (JSD) between CS and nonCS images. Experimental results show that our method can effectively synthesize a large variety of mp-MRI images which contain meaningful CS PCa lesions, display a good visual quality and have the correct paired relationship between the two modalities of a pair. Compared to the state-of-the-art methods based on adversarial learning (Liu and Tuzel, 2016; Costa et al., 2017), our method achieves a significant improvement in terms of both visual quality and several popular quantitative evaluation metrics.

8.
Plant J ; 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31647592

RESUMO

Intronic transposable elements (TEs) comprise a large proportion in eukaryotic genomes, but how they regulate the host genes remains to be explored. Our forward genetic screen disclosed the plant specific RNA polymerases IV and V in suppressing intronic TE-mediated cryptic transcription initiation of a chimeric transcripts at FLC (FLCTE ). Initiation of FLCTE transcription is blocked by the locally formed intronic heterochromatin, which is directly associated with RNA Pol V to inhibit the entry of RNA Pol II and the occupancy of H3K4 methylation. Genome-wide Pol II Ser5p native elongation transcription sequencing revealed that a significant number of intronic heterochromatin-containing genes undergo this mechanism. This study sheds light on deeply understanding the function of intronic heterochromatin on host genes expression in eukaryotic genome.

9.
J Hazard Mater ; : 121483, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31648891

RESUMO

The rational design of novel adsorption materials is imperative to remove toxic metal species from the polluted water. Herein, a core@shell structural Fe-Fe2O3@poly (hexachlorocyclotriphosphazene-co-polyethylenimine) (Fe-Fe2O3@PHCP) magnetic nanochain with high saturation magnetization was fabricated and used for effective adsorption and reduction of hexavalent chromium. The morphology and microstructure of Fe-Fe2O3@PHCP were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The effects of concentration, pH, contact time, temperature and coexisting ions on Cr (VI) removal were studied. Four kinetic models (pseudo-first-order, pseudo-second-order, Bangham and intraparticle diffusion models) and two isotherm models (Freundlich and Langmuir) were used to fit experimental data. Results show the adsorption capacity of Fe-Fe2O3@PHCP for Cr (VI) is up to 229.0 mg g-1. The excellent performance was ascribed to the favorable reduction of Cr (VI) to Cr (III), followed by the chelation of Cr (III) with imino groups. Meanwhile, the residual Cr (VI) were adsorbed on protonated amino and imino groups. The adsorption process is exothermic and spontaneous and nicely follows pseudo-second-order kinetics, intraparticle diffusion model and Langmuir isotherm model. These results indicated that easily separable Fe-Fe2O3@PHCP magnetic nanochains could be a promising adsorbent to remediate chromate wastewater.

10.
11.
J Acoust Soc Am ; 146(3): 1556, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31590542

RESUMO

Temperature-induced variations of elastic moduli in solid media are generally characterized by a strong nonlinear dependence on temperature associated with complex deformations under thermal treatments. Conventional thermoelasticity with third-order elastic constants for the one-order temperature dependence has been extensively studied for crystals, but encountering problems of divergent and limited velocity variations for rocks as a polycrystal mixture, especially at high temperatures. The extension of the theory beyond high-order elastic constants to solid media is addressed in this article to describe the nonlinear temperature dependence of both elastic constants and wave velocities. The total strain is divided into the background component associated with temperature variations and the infinitesimal component induced by propagating waves. A third-order temperature dependence of velocity variations is formulated by taking into account fourth-order elastic constants. Applications to solid rocks (sandstone, granite, and olivine) demonstrate an accurate description of temperature-induced variations, especially for high temperatures. Unlike crystals, the synthetic averaging elastic constants for a solid rock (as a polycrystal mixture) change less than 10% with temperatures. The thermal sensitivity of P-wave velocities is much more than that of S-wave velocities over the vast majority of temperatures examined.

12.
J Chem Phys ; 151(13): 134309, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31594318

RESUMO

The intermediate state of a correlated triplet pair [1(TT)] is pivotal for understanding the mechanism of the singlet fission process in molecule systems. Recently, a spatially separated state of a triplet pair [1(T⋯T)] has been proposed to drive the dissociation of exchange-coupled 1(TT) into free triplets. Here, we study the correlated triplet pairs by monitoring quantum beats in delayed fluorescence from tetracene crystals upon applying magnetic fields of different amplitudes and alignments. We argue that the triplet pairs probed by the quantum beat spectrum are weakly coupled, i.e., the spatially separated 1(T⋯T) state. The experimental data suggest the existence of a direct channel of S1 → 1(T⋯T) in addition to the widely accepted channel of S1 → 1(TT) → 1(T⋯T) for singlet fission. Our work suggests that the quantum beat spectrum is a useful tool to directly probe the 1(T⋯T) state which is valuable for elucidating the intrinsic mechanism of singlet fission.

13.
Chin Med Sci J ; 34(3): 194-198, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31601302

RESUMO

Objective To depict imaging anatomy of bronchial artery (BA) using multidetector CT-angiography (MDCTA) and evaluate the value of MDCTA for management of hemoptysis patients requiring admission to emergency room. Methods We retrospectively studied the clinical and radiological data of patients with severe hemoptysis (≥100 ml of expectorated blood in a 24-hour period) requiring admission to emergency room from Jan 1, 2013 to Dec 31, 2015. Patients' images of MDCTA, treatment modalities, and outcome were discussed. Results A total of 108 patients underwent MDCTA scans. Etiology of hemoptysis was mainly bronchiectasis (44%), tuberculosis sequelae (26%) and tumor (18%). MDCTA visualized 197 traceable BAs and also suggested the involvement of 35 nonbronchial systemic arteries. The mean diameter of BAs, measured at the level of the bronchial bifurcation in the mediastinum, was 2.8±1.2 mm. The mean diameter of BAs, for 52 patients who only received conservative treatment, was 2.9±1.1 mm, and was not significantly larger than that of BAs for 56 patients who underwent bronchial artery embolization (BAE) for continued bleeding which did not resolve after conservative treatment (2.7±1.1 mm, P = 0.94). The technical success rate of embolization was 95% (53/56). Clinical success rate during follow-up was achieved in 50 (94%) of 53 patients who had undergone embolization. Conclusions MDCTA provides useful information for identifying the anatomical characteristics of bleeding-related BAs and nonbronchial systemic arteries for the management of patients with severe hemoptysis. However, MDCTA could not determine the individuals who need BAE through measuring diameter of BAs.

14.
J Clin Lab Anal ; : e23042, 2019 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-31587366

RESUMO

OBJECTIVE: To investigate the correlation of 5-hydroxy tryptamine receptor 6 (5-HTR6) gene polymorphism with vestibular migraine (VM). METHODS: A total of 92 VM patients were enrolled as the observation group, and 100 healthy people receiving physical examinations as the control group. Their general clinical information was collected, and the level of 5-HT in plasma and the vestibular function test indexes were detected. Moreover, the polymorphism of 5-HTR6 rs770963777 was detected with the TaqMan-MGB probe. RESULTS: The observation group had a lower level of 5-HT than the control group (P < .05), and the abnormality rates of the vestibular function tests, including the caloric test, head-shaking test, and vestibular autorotation test, were obviously higher than those in the control group (P < .01). The comparisons showed that the distribution frequencies of the genotypes and alleles were different between the two groups (P < .05). According to the analysis of the genetic mode, there were differences in recessive and additive modes between the two groups (P < .05), but the dominant mode was not different between the two groups (P > .05). CONCLUSION: The level of 5-HT and the vestibular function test indexes can serve as the effective indicators for observing VM, and the polymorphism of 5-HTR6 rs770963777 site is correlated with VM onset.

15.
Biomed Pharmacother ; 120: 109475, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31580970

RESUMO

BACKGROUND: Doxorubicin (DOX) is an anticancer drug that has been widely used in the clinic. However, recently its application has been limited due to the cardiotoxic effects it has caused. Severe cardiotoxicity of DOX causes cardiac hypertrophy that may lead to heart failure. It has previously been demonstrated that CACNA1H is re-expressed in hypertrophic cardiomyocytes. In this study, we aimed to investigate the role of CACNA1H in DOX-induced acute cardiotoxicity, and to investigate its possible underlying mechanisms of action involved. METHODS: Firstly, DOX-induced cardiac injury and changes in the expression of CACNA1H were evaluated. We explored the role of endoplasmic reticulum (ER) stress and apoptosis in mice that underwent DOX-induced cardiac injury. Next, to explore the role of CACNA1H in this process, we evaluated the changes in DOX-induced cardiac injury and ER stress after treatment with the CACNA1H specific inhibitor ABT-639. Next, we used ER stress inhibitor UR906 to verify the role of ER stress in DOX induced cardiotoxicity in H9C2 cells. RESULTS: DOX-treatment caused acute heart injury, leading to a decrease in cardiac function in mice, an increase in apoptosis of cardiac myocytes, and a significant increase in the expression level of CACNA1H in heart tissue. Next, mice were treated with CACNA1H inhibitor ABT-639 and we demonstrated that it partly protects myocardial function and reduces myocardial cell apoptosis. In addition, our data indicated that CACNA1H may play a role in alleviating DOX-induced cardiotoxicity by reducing the severity of ER stress because the use of ABT-639 significantly changed ER stress-related proteins, including p-PERK, PERK, CHOP, GRP78, ATF6, and ATF4. Furthermore, we found that the use of ER stress inhibitor UR906 in H9C2 cells significantly alleviated the increased expression of ER stress related proteins and apoptosis related proteins caused by DOX, and meanwhile reduced the degree of intracellular oxidative stress and intracellular calcium ion concentration. CONCLUSION: CACNA1H inhibitors significantly alleviated DOX-induced cardiotoxicity and apoptosis induced by ER stress.

16.
Neurochem Res ; 44(11): 2536-2545, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31529334

RESUMO

Dehydrocorybulbine (DHCB), an alkaloid from Corydalis yanhusuo. W.T, has been identified as a dopamine receptor antagonist. We extended our assessment of its pharmacological profile and found that DHCB exhibits high to moderate binding affinities to sigma 1 and 2 receptors, serotonin 5-HT7 receptor, and histamine H2 receptors. This led us to evaluate DHCB properties in pharmacological (apomorphine and MK-801) animal models of schizophrenia in mice. The pharmacological profile of DHCB was screened through radioligand receptor binding assays. Single dose of DHCB reversed the locomotor hyperactivity, stereotypy, and prepulse inhibition deficits induced by the dopaminergic agonist apomorphine. DHCB also reversed the depressive-like behavior and memory deficit induced by the glutamatergic antagonist MK-801 in the forced swim and the novel object recognition assays, respectively. These results indicate that DHCB effectively improves schizophrenia-like behavioral deficits that are induced by the disruption of dopaminergic and glutamatergic systems. The effectiveness of DHCB in reversing responses that mimic negative and cognitive deficits of schizophrenia might suggest that its anti-schizophrenia effects are mediated through modulating the activities of several receptor particularly sigma 1, sigma 2, 5-HT7 and dopamine receptors. Our study casts DHCB as a promising lead for therapeutic treatment of schizophrenia.

17.
Sci Total Environ ; 697: 133952, 2019 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-31487587

RESUMO

Climate is the fundamental determinant of plant metabolism and net primary productivity (NPP). However, whether climate drives NPP directly or indirectly is not well understand. The Gobi desert across a precipitation gradient in the arid zone provides an ideal naturally-controlled platform for studying the precipitation-productivity relationships. We conducted 3-year experiments in four Gobi desert shrublands across an aridity gradient in Gansu Province of China to test the relationship between water availability and shrub productivity as well as the relative importance of the possible factors driving productivity (using piecewise structural equation modeling) and to explore the appropriate variables for predicting productivity (using three spatial models). The results showed that water availability indirectly affected the NPP via stand biomass, while stand biomass had a significant direct effect on NPP regardless of whether the leaf water content and stand height were considered. The model based on stand size (71.6%) and the model that contained both stand size and water availability (72.3%) explained more of the variation in the water-NPP relationships than the model based on water availability (37.3%). Our findings suggest that even in extremely water-limited areas, the effects of water availability on plant growth and the kinetics of plant metabolism could be indirect via plant size, demonstrating the importance of plant size as an indicator of shrub productivity. This study explains the mechanisms underlying the NPP driving pattern and proposes a practical NPP model for arid ecosystems.

18.
J Agric Food Chem ; 67(40): 10997-11004, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31487170

RESUMO

The enantioselective bioactivity, toxicity, and environmental behaviors of isocarbophos (ICP) were investigated. The order of the bioactivity and toxicity was S-(+) ≥ rac > R-(-), and the difference of R-(-) and S-(+) was up to 232 times. The usage of S-(+)-ICP may efficiently reduce the usage amount of rac-ICP by 35% under the same effect, and the toxicity was not increased. Based on the toxic unit analysis, the additive effect and synergistic effect of ICP enantiomers were found in the four nontarget organisms, and R-(-)-ICP might cooperate the side-effects of S-(+)-ICP. The accumulation of rac-ICP in earthworms was enantioselective with an enantioenrichment of R-(-)-ICP, so the usage of racemic ICP might increase the exposure risk of R-(-)-ICP to earthworms. From the comprehensive results, the production of enantiomer enriched S-(+)-ICP might increase bioactivity and reduce environmental pollution, while the toxicity of S-(+)-ICP to other nontarget organisms needs to be further assessed.


Assuntos
Inseticidas/química , Inseticidas/toxicidade , Malation/análogos & derivados , Animais , Malation/química , Malation/toxicidade , Oligoquetos/efeitos dos fármacos , Oligoquetos/crescimento & desenvolvimento , Estereoisomerismo , Relação Estrutura-Atividade
19.
Cancer Lett ; 466: 61-70, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31546023

RESUMO

F-box proteins (FBPs) are proteins containing an F-box domain and are one of three subunits in SKP1-Cullin1-F-box protein (SCF) E3 ligase complexes. Accumulated evidence has shown that FBP regulates tumorigenesis and the progression of human cancer via ubiquitination and degradation of downstream substrates, which can be oncoproteins or tumor suppressors. Emerging evidence has revealed that noncoding RNAs (ncRNAs) govern the expression of FBP in human cancers. Specifically, microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs) have been shown to govern FBP expression in malignancies. Therefore, in this review article, we discuss how miRNAs target FBPs and participate in tumorigenesis and tumor progression. Moreover, we briefly highlight the role of lncRNAs and circRNAs in the regulation of FBPs in cancer. Therefore, targeting ncRNAs could be a novel approach to regulate FBPs for anticancer therapy.

20.
J Cell Biochem ; 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31498488

RESUMO

Emerging evidence has indicated that long noncoding RNA (lncRNA) plays crucial roles in regulating hepatocellular carcinoma (HCC) progression. However, a comprehensive lncRNA-transcription factor (TF)-message RNA (mRNA) in HCC remains absent. The aim of this study was to identify aberrantly expressed lncRNAs and the associated TF-mRNA network in HCC. GSE124535 dataset was downloaded from the Gene Expression Omnibus database. Limma package in R was conducted to analyze abnormally expressed lncRNAs (differentially expressed lncRNAs [DELs]) and mRNAs (differentially expressed genes). The prognostic roles of screened DELs in HCC were evaluated at GEPIA. The expression of DELs that correlated with overall survival of HCC patients was further validated using circlncRNAnet, GEPIA, and StarBase. LncRNA-TF-mRNA potential triplets in HCC were predicted by LncMAP. After that, lncRNA-TF-mRNA networks were established using Cytoscape. A total of 20 upregulated and 17 downregulated DELs were identified in HCC tissues compared with adjacent noncancerous tissues. Six out of 20 upregulated and 6 out of 17 downregulated DELs were identified have significant impact on the overall survival of HCC patients. Results in circlncRNAnet, GEPIA, and Starbase confirmed the expression level of DELs obtained from GSE124535. Finally, the LncRNA-TF-mRNA regulatory networks for upregulated and downregulated lncRNA were established based on these analyses results. Among these lncRNAs in the network, the aberrantly expression of lncRNAs including LINC00511, RP11-290F5.1, MIR4435-2HG, and CTC-537E7.3 in HCC was first time reported to date. In the study, potential LncRNA-TF-mRNA regulatory networks were identified, which will advance our understanding concerning the progression of HCC.

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