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1.
Reproduction ; 161(1): 11-19, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33112285

RESUMO

Endometriosis is a common gynecological disease in reproductive-age women. Although the hormone-dependent therapy is the first line treatment for endometriosis, it is not a curative regimen and associated with severe side-effects, which significantly decrease the life quality of affected individuals. To seek a target for treatment of endometriosis, we focused on plasma membrane proteins that are elevated in ectopic cells and exert beneficial effects in cell growth and survival. We performed bioinformatics analysis and identified the neurotrophic receptor tyrosine kinase 2 (NTRK2) as a potential candidate for treatment. The expression levels of NTRK2 were markedly upregulated in the lesions of clinical specimen as well as in the mouse endometriotic-like lesion. Mechanistic investigation demonstrated that upregulation of NTRK2 is induced by hypoxia in a hypoxia-inducible factor 1 alpha-dependent manner. Knockdown of NTRK2 or administration of ANA-12, a selective antagonist of NTRK2, significantly induced endometriotic stromal cells death, suggesting it may be a potential therapeutic agent. In vivo study using surgery-induced endometriosis mice model showed ANA-12 (1.5 mg/kg body weight) treatment induced apoptosis of endometriotic cells and caused the regression of ectopic lesions. Taken together, our findings suggest a possible mechanism responsible for the aberrant expression of NTRK2 in endometriotic lesions and this may be involved in the pathogenesis of endometriosis.

2.
Nanotechnology ; 32(1): 015503, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32947275

RESUMO

High-performance solar-blind separate absorption and multiplication avalanche photodiodes (SAM-APDs) were fabricated based on a p-NiO/MgO/n-ZnO dual heterojunction structure. The prepared SAM-APDs exhibited a separated absorption and multiplication structure that used NiO and ZnO as absorption layers, and ultrawide-bandgap MgO as a multiplication layer. When the reverse-bias voltage exceeded 6 V, carrier avalanche multiplication occurred, and the avalanche gain reached a high value of 2.7 × 103, corresponding to a 1120% quantum efficiency, at a reverse-bias voltage of 10 V. These solar-blind SAM-APDs had an ultraviolet (UV) (310 nm)/visible (500 nm) rejection ratio as high as 563.6 at a 2 V reverse-bias voltage. These features render the SAM-APDs highly suitable for practical applications as UV solar-blind photodetectors.

3.
Chemosphere ; 262: 127772, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32799140

RESUMO

This study utilized the freshwater amphipod (Hyalella azteca) for the indication of contamination risk levels of sediment-associated contaminants in the Erren (ER1∼ER10) and Sanye Rivers (SY1∼SY5) which were contaminated by metal scrap and smelting industries for decades. Toxicity identification evaluations involving the manipulation of pore water and whole-sediment samples were conducted to identify causative pollutants. Impacts on the aquatic environment were then evaluated in order to explore how industrial development led to contaminant accumulation in sediments and resulted in biological effects. A whole-sediment TIE indicated that the major toxicant at sampling sites ER8 and SY5 was ammonia and that its toxicity was significantly reduced by the addition of zeolite. Toxicity at sampling sites ER4 and ER9 was induced by ammonia and heavy metals (Zn, Cd, Cr, As), whereas Cr was at toxic levels at ER6. ∑PAHs was another major class of contaminants at site ER2. Metals (Zn, Ni, Pb, Cd, Cr, and As) were identified as major toxic contaminants at three sites (ER3, SY1, and SY3). The application of TIEs confirmed that a causative toxicant can be identified and that its measured toxicity correlated with its concentration. In conclusion, a TIE approach was successful in demarcating most effective contaminant groups (ammonia, heavy metals, and non-polar organic compounds) in whole-sediment cores, their porewaters and potential toxicities from a highly polluted river after remediation in southern Taiwan to an invertebrate animal model H. azteca.


Assuntos
Anfípodes/efeitos dos fármacos , Monitoramento Ambiental/métodos , Sedimentos Geológicos/química , Rios/química , Poluentes Químicos da Água/toxicidade , Amônia/análise , Amônia/toxicidade , Animais , Água Doce/química , Metais Pesados/análise , Metais Pesados/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Taiwan , Clima Tropical , Poluentes Químicos da Água/análise , Zeolitas/química
4.
Ear Nose Throat J ; : 145561320972608, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33155834

RESUMO

BACKGROUND: An outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 infection occurred in Wuhan, China, in December 2019. To date, the analysis of fatal cases and the risk factors for death have rarely been reported. METHODS: In this study, 220 adult patients with confirmed and suspected COVID-19 were enrolled. Clinical characteristics, laboratory data, treatments, and complications were compared between 168 survivors and 52 nonsurvivors. Univariable analysis and multivariable logistic regression were used to investigate the risk factors for mortality. RESULTS: A total of 220 patients (168 were discharged and 52 died in the hospital) were enrolled in the study. The median age of all patients was 59.5 (47.0-69.0) years, and the median age of patients who died was significantly older than that of patients who survived (70.5 vs 56.0 years, respectively; P < .001). According to multivariate logistic regression, older age (odds ratio: 1.09, 95% CI: 1.03-1.15; P = .001), initial Sequential Organ Failure Assessment (SOFA) score >2 (37.4, 9.4-148.0; P = .011), and respiratory rate >24 per minute (10.89, 1.47-80.67; P = .019) were independent risk factors for mortality. CONCLUSION: Clinical and laboratory parameters predicting poor prognosis including older age, baseline SOFA score >2, and respiratory rate >24 per minute were identified.

5.
Sex Transm Infect ; 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33219164

RESUMO

OBJECTIVES: Co-infection of syphilis and HIV remains hard to manage and its morbidity shows a rising tendency. Syphilis has been associated with increased risk of HIV acquisition in high-risk groups, especially in men who have sex with men (MSM). This systematic review and meta-analysis estimates the effect of syphilis infection on subsequent HIV acquisition, and assesses its difference between MSM and other high-risk populations. METHODS: Five electronic databases were searched for literature published to 21 September 2019 without language restrictions. Longitudinal studies that enrolled key populations to compare the incidence of HIV with and without syphilis exposure were included. We used a random-effects model to estimate the effect of syphilis infection on HIV acquisition among high-risk populations, which include MSM, sex workers, serodiscordant couples, people who inject drugs and attendees of STD clinics. RESULTS: A total of 17 cohorts and 5 case-control studies involving 65 232 participants were included. HIV incidence showed a two-time increase after syphilis exposure, compared with a control group (relative risk (RR) 2.67 (95% CI 2.05 to 3.47); p<0.05 for prevalence; RR 3.21 (95% CI 2.26 to 4.57); p=0.419 for incidence). No significant differences were observed between MSM and other high-risk groups in syphilis infection prevalence (RR 2.60 (95% CI 1.78 to 3.80); p<0.05 vs RR, 2.98 (95% CI 2.15 to 4.14); p<0.05; ratio of relative risk 0.76 (95% CI 0.49 to 1.17)). CONCLUSIONS: Syphilis infection increases the risk of HIV acquisition in high-risk populations. There is no evidence to suggest MSM are at greater risk than other high-risk populations. Prompt diagnosis, timely treatment, preventive interventions against syphilis infection would be a worthwhile investment for reducing HIV incidence. Strategies to combat stigma and discrimination targeted at MSM are pragmatically needed.

6.
Sci Rep ; 10(1): 19695, 2020 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-33184436

RESUMO

Bladder cancer is one of the most common cancers worldwide. The immune response and immune cell infiltration play crucial roles in tumour progression. Immunotherapy has delivered breakthrough achievements in the past decade in bladder cancer. Differentially expressed genes and immune-related genes (DEIRGs) were identified by using the edgeR package. Gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for functional enrichment analysis of DEIRGs. Survival-associated IRGs were identified by univariate Cox regression analysis. A prognostic model was established by univariate COX regression analysis, and verified by a validation prognostic model based on the GEO database. Patients were divided into high-risk and low-risk groups based on the median risk score value for immune cell infiltration and clinicopathological analyses. A regulatory network of survival-associated IRGs and potential transcription factors was constructed to investigate the potential regulatory mechanisms of survival-associated IRGs. Nomogram and ROC curve to verify the accuracy of the model. Quantitative real-time PCR was performed to validate the expression of relevant key genes in the prognostic model. A total of 259 differentially expressed IRGs were identified in the present study. KEGG pathway analysis of IRGs showed that the "cytokine-cytokine receptor interaction" pathway was the most significantly enriched pathway. Thirteen survival-associated IRGs were selected to establish a prognostic index for bladder cancer. In both TCGA prognostic model and GEO validation model, patients with high riskscore had worse prognosis compared to low riskscore group. A high infiltration level of macrophages was observed in high-risk patients. OGN, ELN, ANXA6, ILK and TGFB3 were identified as hub survival-associated IRGs in the network. EBF1, WWTR1, GATA6, MYH11, and MEF2C were involved in the transcriptional regulation of these survival-associated hub IRGs. The present study identified several survival-associated IRGs of clinical significance and established a prognostic index for bladder cancer outcome evaluation for the first time.

7.
J Proteome Res ; 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33231461

RESUMO

Antibiotic-resistant bacteria are a serious threat to human and animal health. Metabolite-enabled eradication of drug-resistant pathogens is an attractive strategy, and metabolite adjuvants, such as fumarate, are used for restoring the bactericidal ability of antibiotics. However, we show that metabolites in the TCA cycle increase the viability of Edwardsiella tarda against chloramphenicol (CAP), based on the survival assay of differential metabolites identified by LC-MS/MS. Furthermore, NADPH promotes CAP resistance in the CAP-resistant strain, while oxidants restore the bactericidal ability. Finally, we show that the intracellular redox state determines the sensitivity to CAP, and the total antioxidative capacity is decreased significantly in the antibiotic-resistant strain. Considering that the metabolites promote CAP resistance, metabolite adjuvants should be applied very cautiously. Overall, our research expands on the knowledge that the redox state is related to the bactericidal ability of CAP.

8.
Clin Med (Lond) ; 20(6): 590-592, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-33199326

RESUMO

While clinical environments are highly focused on COVID-19, reports of missed or delayed treatment for conditions that imitate COVID-19, such as pneumonia caused by the fungus Pneumocystis jirovecii, are emerging. Given the uncertain spectrum of COVID-19 presentations and variable sensitivity of laboratory tests for SARS-CoV-2, there is a risk that, without a high index of suspicion, alternative aetiologies may be overlooked while pursuing a diagnosis of COVID-19. The British HIV Association has been calling for the inclusion of HIV testing in all patients admitted to hospital with suspected COVID-19. In this article we reflect on the importance of including HIV testing to prevent avoidable morbidity and mortality in our patients.

9.
J Affect Disord ; 280(Pt A): 156-166, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33212407

RESUMO

BACKGROUND: Women with threatened preterm labor (TPTL) resting at home face several life challenges, but their psychological well-being has been ignored. This study aimed to explore the status and related factors of the psychological status of women with TPTL resting at home, and to compare the psychological status differences between TPTL and healthy pregnant women. METHODS: A cross-sectional, multiple time-point study was conducted to repeatedly assess depression, perceived stress, and distress in 49 TPTL women and 62 healthy pregnant women during pregnancy in Taiwan. Mann-Whitney U tests were used to compare the psychological status differences between these women and the generalized estimating equation was used to identify the factors related to their psychological status. RESULTS: The TPTL women's perceived stress at 24-27 weeks (p=0.047) and 32-35 weeks (p=0.04) and distress at each time point was significantly greater than that of healthy pregnant women (p<0.001). The common distress experienced by TPTL women was the inability to provide self-care and family care, the baby's health and safety, and to request leave from work for bed rest. Positive personalities, gestational age, preterm birth history, follow-up status and employment have been shown to be related to the psychological status of TPTL women. LIMITATIONS: The major limitation of this study is the small sample size. CONCLUSION: This study contributed to a better understanding of the emotional burdens of women with TPTL resting at home. Such findings highlight the need for constructing effective interventions to alleviate the psychological burden of these women.

10.
Nat Commun ; 11(1): 5974, 2020 11 25.
Artigo em Inglês | MEDLINE | ID: mdl-33239627

RESUMO

The lateral parabrachial nucleus (LPBN) is known to relay noxious information to the amygdala for processing affective responses. However, it is unclear whether the LPBN actively processes neuropathic pain characterized by persistent hyperalgesia with aversive emotional responses. Here we report that neuropathic pain-like hypersensitivity induced by common peroneal nerve (CPN) ligation increases nociceptive stimulation-induced responses in glutamatergic LPBN neurons. Optogenetic activation of GABAergic LPBN neurons does not affect basal nociception, but alleviates neuropathic pain-like behavior. Optogenetic activation of glutamatergic or inhibition of GABAergic LPBN neurons induces neuropathic pain-like behavior in naïve mice. Inhibition of glutamatergic LPBN neurons alleviates both basal nociception and neuropathic pain-like hypersensitivity. Repetitive pharmacogenetic activation of glutamatergic or GABAergic LPBN neurons respectively mimics or prevents the development of CPN ligation-induced neuropathic pain-like hypersensitivity. These findings indicate that a delicate balance between excitatory and inhibitory LPBN neuronal activity governs the development and maintenance of neuropathic pain.

11.
Oncogene ; 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-33191406

RESUMO

Ribonucleotide reductase (RNR), which is a heterodimeric tetramer composed of RRM1 and RRM2 subunits, is the rate-limiting enzyme in the synthesis of deoxyribonucleoside triphosphates (dNTPs) and essential for both DNA replication and the repair of DNA damage. The activity of RNR is coordinated with the cell cycle and regulated by fluctuations in the level of the RRM2 subunit. Multiple cancer types, including Ewing sarcoma tumors, are sensitive to inhibitors of RNR or a reduction in the levels of either the RRM1 or RRM2 subunits of RNR. Here, we show that the expression of the RRM2 protein is dependent on active protein synthesis and that 4E-BP1, a repressor of cap-dependent protein translation, specifically regulates the level of the RRM2 protein. Furthermore, inhibition of mTORC1/2, but not mTORC1, activates 4E-BP1, inhibits protein synthesis, and reduces the level of the RRM2 protein in multiple sarcoma cell lines. This effect of mTORC1/2 inhibitors on protein synthesis and RRM2 levels was rescued in cell lines with the CRISPR/Cas9-mediated knockout of 4E-BP1. In addition, the inducible expression of a mutant 4E-BP1 protein that cannot be phosphorylated by mTOR blocked protein synthesis and inhibited the growth of Ewing sarcoma cells in vitro and in vivo in a xenograft. Overall, these results provide insight into the multifaceted regulation of RRM2 protein levels and identify a regulatory link between protein translation and DNA replication.

12.
Mar Pollut Bull ; : 111797, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33183750

RESUMO

This study reports on a sediment toxicity test developed with a native amphipod strain of Ptilohyale barbicornis where sediments from 10 estuarine stations along the western coastline of Taiwan were evaluated with respect to trace metals and PAHs. The test was validated by determining dose-response relationships for aqueous copper, and cadmium and sodium dodecyl sulfate as well as copper-spiked sediment, showing a sensitivity of P. barbicornis indicating its capability serving as a toxicity test species. A significant negative correlation between growth effects after 28 days of exposure to field-collected contaminated sediments and PAHs concentrations was observed. Similarly, the chronic toxicity test showed that growth was mostly inhibited compared to controls, body lengths in particular being significantly different from controls (p < 0.05). Various estuarine sediment pollutants in the quality guidelines can be further evaluated using P. barbicornis to understand their comprehensive biological effects and ecological risks.

13.
Invest New Drugs ; 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33215325

RESUMO

The acceleration of therapeutic antibody development has been motivated by the benefit to and their demand for human health. In particular, humanized transgenic antibody discovery platforms, combined with immunization, hybridoma fusion and/or single cell DNA sequencing are the most reliable and rapid methods for mining the human monoclonal antibodies. Human GPC3 protein is an oncofetal antigen, and it is highly expressed in most hepatocellular carcinomas and some types of squamous cell carcinomas. Currently, no fully human anti-GPC3 therapeutic antibodies have been reported and evaluated in extensive tumor tissues. Here, we utilized a new humanized transgenic mouse antibody discovery platform (CAMouse) that contains large V(D)J -regions and human gamma-constant regions of human immunoglobulin in authentic configurations to generate fully human anti-GPC3 antibodies. Our experiments resulted in four anti-GPC3 antibodies with high-specific binding and cytotoxicity to GPC3 positive cancer cells, and the antibody affinities are in the nanomolar range. Immunohistochemistry analysis demonstrated that these antibodies can recognize GPC3 protein on many types of solid tumors. In summary, the human anti-human GPC3 monoclonal antibodies described here are leading candidates for further preclinical studies of cancer therapy, further, the CAMouse platform is a robust tool for human therapeutic antibody discovery.

14.
Huan Jing Ke Xue ; 41(11): 5168-5175, 2020 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124261

RESUMO

In this study, a continuous rape-rice rotation plot experiment was conducted over three years. Repair materials were continuously applied in the first two years, and no repair materials were applied in the second year. The repair effects of hydroxyapatite, lime, biochar, bio-organic fertilizer, and nano-materials on copper contaminated soil and the enrichment of copper in different parts of rape and rice were investigated. The results show that hydroxyapatite, lime, and nanomaterials can significantly increase soil pH, and different restoration materials can effectively inhibit the movement of soil copper. The effective copper treatment with lime restoration soil had the largest decrease. The four seasons of continuous application of restoration materials were 38.9%, 34.9%, 27.88%, and 29.04%, respectively, and the subsequent effect of lime passivation of effective copper was better than other restoration materials. The application of the repair material significantly reduced the copper content in edible parts of rape and rice. In the four seasons of application of the repair material, the maximum copper content in edible parts of different crops decreased by 46.03%, 22.2%, 29.44%, and 31.71%, respectively. Due to the application effect of the repair material, the copper content in the edible part of the two season crops, without the repair material, did not exceed the national food safety limit. With the use of different repair materials, the yields of rapeseed and rice were improved. This test can provide some theoretical basis and technical support for soil improvement in copper-contaminated areas.


Assuntos
Recuperação e Remediação Ambiental , Oryza , Poluentes do Solo , Cádmio/análise , Carvão Vegetal , Cobre , Rotação , Solo , Poluentes do Solo/análise
15.
JMIR Med Inform ; 8(10): e20291, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33084582

RESUMO

BACKGROUND: Many drugs do not work the same way for everyone owing to distinctions in their genes. Pharmacogenomics (PGx) aims to understand how genetic variants influence drug efficacy and toxicity. It is often considered one of the most actionable areas of the personalized medicine paradigm. However, little prior work has included in-depth explorations and descriptions of drug usage, dosage adjustment, and so on. OBJECTIVE: We present a pharmacogenomics knowledge model to discover the hidden relationships between PGx entities such as drugs, genes, and diseases, especially details in precise medication. METHODS: PGx open data such as DrugBank and RxNorm were integrated in this study, as well as drug labels published by the US Food and Drug Administration. We annotated 190 drug labels manually for entities and relationships. Based on the annotation results, we trained 3 different natural language processing models to complete entity recognition. Finally, the pharmacogenomics knowledge model was described in detail. RESULTS: In entity recognition tasks, the Bidirectional Encoder Representations from Transformers-conditional random field model achieved better performance with micro-F1 score of 85.12%. The pharmacogenomics knowledge model in our study included 5 semantic types: drug, gene, disease, precise medication (population, daily dose, dose form, frequency, etc), and adverse reaction. Meanwhile, 26 semantic relationships were defined in detail. Taking melanoma caused by a BRAF gene mutation into consideration, the pharmacogenomics knowledge model covered 7 related drugs and 4846 triples were established in this case. All the corpora, relationship definitions, and triples were made publically available. CONCLUSIONS: We highlighted the pharmacogenomics knowledge model as a scalable framework for clinicians and clinical pharmacists to adjust drug dosage according to patient-specific genetic variation, and for pharmaceutical researchers to develop new drugs. In the future, a series of other antitumor drugs and automatic relation extractions will be taken into consideration to further enhance our framework with more PGx linked data.

16.
PLoS One ; 15(10): e0239723, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33006996

RESUMO

BACKGROUND: Allergic rhinitis (AR) is a burdensome respiratory disorder whose etiology and pathophysiology remain controversial and most likely multifactorial. Accumulated evidence indicates that gut dysbiosis contributes to AR via the gut-airway axis. Constipation could result in alteration of the intestinal microflora. The clinical impact of constipation on AR has not been studied. We aimed to evaluate the risk of AR in constipated patients using a nationwide longitudinal population-based cohort. METHODS: We identified 57786 patients with constipation and 57786 matched controls between 1999 and 2013 from the Longitudinal Health Insurance Database, which is a subset of Taiwanese National Health Insurance Research Database. Propensity score analysis was used for matching age, sex, comorbidities, and medications at a ratio of 1:1. Multiple Cox regression and subgroup analyses were used to estimate the adjusted hazard ratio of AR. RESULTS: The incidence of AR was 32.2 per 1,000 person-years in constipated patients, which was twice that of non-constipated patients. After adjustment for patients' age, gender, comorbidities, and medications, patients with constipation had a 2.3-fold risk of AR compared to those without constipation (adjusted hazard ratio [aHR]: 2.30; 95% CI, 2.23-2.37). In subgroup analyses, patients aged 20-39 years had a 2.24-fold higher risk of AR in the constipation cohort (aHR; 95% CI, 2.12-2.36). Patients aged <20, 40-64, and ≥65 years had a 2.09, 2.05, and 2.07-fold risk of AR in the constipation cohort, respectively (aHR; 95% CI, 1.98-2.20, 1.94-2.18, and 1.92-2.23). Also, patients with constipation had a higher likelihood of AR, regardless of sex, and with or without comorbidities including hyperlipidemia, hypertension, chronic kidney disease, chronic liver disease, diabetes, chronic obstructive pulmonary disease, rheumatoid arthritis, dyspepsia, irritable bowel syndrome, and anxiety. CONCLUSION: Constipation might be associated with an increased risk of incidental AR. It seems that physicians should keep a higher index of suspicion for AR in people with constipation. The patency issue of gut could not be ignored in patients with AR.


Assuntos
Constipação Intestinal/fisiopatologia , Rinite Alérgica/etiologia , Rinite Alérgica/fisiopatologia , Adulto , Idoso , Estudos de Coortes , Comorbidade , Constipação Intestinal/epidemiologia , Constipação Intestinal/metabolismo , Disbiose , Feminino , Microbioma Gastrointestinal , Humanos , Incidência , Síndrome do Intestino Irritável/epidemiologia , Masculino , Doenças Metabólicas/epidemiologia , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Doença Pulmonar Obstrutiva Crônica/epidemiologia , Insuficiência Renal Crônica/epidemiologia , Rinite Alérgica/epidemiologia , Fatores de Risco , Taiwan/epidemiologia
17.
Innate Immun ; 26(8): 666-682, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33100122

RESUMO

Sepsis is the major cause of mortality in the intensive care unit. The aim of this study was to identify the key prognostic biomarkers of abnormal expression and immune infiltration in sepsis. In this study, a total of 36 differentially expressed genes were identified to be mainly involved in a number of immune-related Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. The hub genes (MMP9 and C3AR1) were significantly related to the prognosis of sepsis patients. The immune infiltration analysis indicated a significant difference in the relative cell content of naive B cells, follicular Th cells, activated NK cells, eosinophils, neutrophils and monocytes between sepsis and normal controls. Weighted gene co-expression network analysis and a de-convolution algorithm that quantifies the cellular composition of immune cells were used to analyse the sepsis expression data from the Gene Expression Omnibus database and to identify modules related to differential immune cells. CEBPB is the key immune-related gene that may be involved in sepsis. Gene set enrichment analysis revealed that CEBPB is involved in the processes of T cell selection, B cell-mediated immunity, NK cell activation and pathways of T cells, B cells and NK cells. Therefore, CEBPB may play a key role in the biological and immunological processes of sepsis.

18.
Medicine (Baltimore) ; 99(41): e22693, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33031340

RESUMO

BACKGROUND: Compared with open lumbar microdiscectomy, percutaneous endoscopic lumbar discectomy (PELD) has the advantages of remarkable preservation of paravertebral structures, less bleeding, shorter operation time and fewer complications, it is a common method for the treatment of lumbar disc herniation (LDH). Local anesthesia is recommended during PELD. However, intraoperative pain is sometimes difficult to control satisfactorily. The efficacy of bilateral intervertebral foramen block (IFB) for pain management in PELD remains unclear. Therefore, this regimen is utilized in a randomized controlled trial for the assessment the safety and effectiveness of bilateral IFB for PELD pain control. METHOD: This is a single center and randomized controlled trial which will be implemented from September 2020 to September 2021. This research protocol is in accordance with the items of the Standard Protocol for Randomized Trials, which was authorized through the Ethics Committee of Huzhou Central Hospital & Affiliated Centre Hospital of Huzhou University (HZCH0465-0864). 100 participants who undergo PELD will be analyzed. Inclusion criteria containsThe exclusion criteria contains:Patients will be randomly divided into bilateral IFB group (with 50 patients) and local infiltration analgesia group (with 50 patients). Primary outcomes are pain score at different time points. The secondary outcomes are the operative time, radiation exposure time, length of hospital stay and postoperative complications. All the analysis is implemented through applying the IBM SPSS Statistics for Windows, version 20 (IBM Corp., Armonk, NY, USA). RESULTS: The clinical outcome variables between groups are illustrated in the Table 1. CONCLUSION: This investigation can offer a reliable basis for the effectiveness and safety of IFB in treating the PELD pain. TRIAL REGISTRATION: This study protocol is registered in Research Registry (researchregistry5985).


Assuntos
Raquianestesia/métodos , Discotomia , Vértebras Lombares/cirurgia , Manejo da Dor/métodos , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
19.
Indoor Air ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33090568

RESUMO

We aimed toevaluate the association of the exposure to environmental tobacco smoke (ETS) during pregnancy and infancy with the risk of upper respiratory tract infections (URTI) in infants based on a Chinese birth cohort study. Among 4178 infants who constituted the final study population, 46.8% experienced URTI in their first year of life. The hazard ratios (HR) and 95% confidence intervals (CI) for the risk of URTI were assessed using Cox regression models. Compared with no ETS during pregnancy, continued ETS during pregnancy was independently associated with a higher risk of URTI (HR: 1.36, 95% CI: 1.13, 1.63) after adjustment for potential confounders, and also associated with earlier occurrence of URTI (log-rank P = 0.002). The association remained consistent across the strata defined according to maternal age, number of siblings, sex, and breastfeeding. Exposure to ETS during infancy was associated with URTI only among infants who were breastfed for less than 12 months (P for interaction < 0.05).Furthermore, infants exposed to ETS during both pregnancy and infancy showed the highest HR of 1.46 (95% CI: 1.16, 1.85) for URTI.Efforts should be made to protect pregnant women and infants from the adverse effects of indoor and outdoor ETS.

20.
Reproduction ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33112784

RESUMO

Endometriosis is a benign gynecological disease that affects about 10% women of reproductive age. Patients with endometriosis suffer from long-term coexistence with dysmenorrhea, dyspareunia, and even infertility, which severely reduces life quality. So far, surgical removal and hormonal medication are the major treatment options; however, high recurrence and severe adverse effects after surgery and hormone treatment, respectively hamper the therapeutic efficacy. Since the etiology of endometriosis is enigmatic, the mechanisms involved in the pathogenesis remains largely unclarified. Hypoxia is a kind of cellular stress that drives the expression of a specific group of genes mainly through the effect of hypoxia inducible factors (HIFs). A growing body of evidence demonstrated that hypoxia plays critical roles in modulating the pathological conditions in endometriosis, such as increasing adhesion ability, causing dysregulation of estrogen biosynthesis, aberrant production of proinflammatory cytokines, and suppression of immune functions. In this review, we summarize the findings of most recent studies in exploring the underlying mechanisms of hypoxia involved in endometriosis. Potential therapeutic options of targeting HIF and downstream effectors will also be discussed.

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