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1.
J Ethnopharmacol ; 282: 114611, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34537280

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ilex pubescens (I. pubescens), has been widely used to treat cardiovascular disease (CVD) in South China. Several studies have revealed aspect of its phytochemistry and pharmacological activities in cardiovascular diseases, but its active compounds and mechanisms of action are still unclear. The aim of this study was to search for the active compounds and the pharmacological mechanisms of I. pubescens for myocardial ischemia-reperfusion injury (MI/RI) by an integrative pharmacology-based investigation. MATERIALS AND METHODS: The main targets of compounds in I. pubescens were predicted using the TargetNet webserver (http://targetnet.scbdd.com). The network between compounds and predicted targets related to MI/RI and compounds was constructed. Functional enrichment analysis was performed to investigate the specific functions and pathways involved in the candidate I. pubescens targets acting on MI/RI, which were further validated by in vitro and in vivo experiments. RESULTS: A total of 191 targets were predicted for 64 chemical compounds in I. pubescens. Following Venn's analysis, we found that 38 candidate targets of I. pubescens were associated with protective effects against MI/RI. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these targets were related to estrogen signaling pathway. Importantly, the cardioprotective effects of I. pubescens and its active compounds were evaluated and the regulatory effects on key targets of heat shock protein 90 alpha family class A member 1 (HSP90AA1) and Estrogen receptor 1 (ESRα) in estrogen signaling pathway were validated in vitro and in vivo. CONCLUSION: Our discoveries revealed that I. pubescens ameliorated MI/RI by regulating HSP90AA1 and ESRα in estrogen signaling pathway.

2.
Med Sci Monit ; 27: e932228, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34593750

RESUMO

BACKGROUND This study assessed magnetic resonance imaging (MRI) of acute and chronic partial anterior cruciate ligament (ACL) tears using maximum knee flexion in the lateral decubitus position compared with routine knee positioning in 204 patients at a single center. MATERIAL AND METHODS Based on the time interval from injury to MRI examination, the 204 patients in this study were divided into 3 groups: subacute (6 weeks to 3 months), intermediate (3 months to 1 year), and chronic (>1 year). All patients received both routine MRI (MRI R) and maximum knee flexion in the lateral decubitus position MRI (MRI S) examination, followed by knee arthroscopy. Three radiologists blinded to patient groups evaluated the MRI scans and made a diagnosis. Results of knee arthroscopy were referenced as the criterion standard. The sensitivity and specificity of MRI R and MRI S groups were calculated and compared. RESULTS The MRI S diagnostic rate was comparable to that of knee arthroscopy. MRI S had significantly higher sensitivity than MRI R for partial ACL tears, especially in the intermediate group (P<0.01). CONCLUSIONS MRI of partial ACL tears using maximum knee flexion in the lateral decubitus position improved the diagnostic rate relative to routine MRI examination, particularly in patients in the intermediate group.

3.
BMC Bioinformatics ; 22(1): 475, 2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34600466

RESUMO

BACKGROUND: Correctly classifying the subtypes of cancer is of great significance for the in-depth study of cancer pathogenesis and the realization of personalized treatment for cancer patients. In recent years, classification of cancer subtypes using deep neural networks and gene expression data has gradually become a research hotspot. However, most classifiers may face overfitting and low classification accuracy when dealing with small sample size and high-dimensional biology data. RESULTS: In this paper, a laminar augmented cascading flexible neural forest (LACFNForest) model was proposed to complete the classification of cancer subtypes. This model is a cascading flexible neural forest using deep flexible neural forest (DFNForest) as the base classifier. A hierarchical broadening ensemble method was proposed, which ensures the robustness of classification results and avoids the waste of model structure and function as much as possible. We also introduced an output judgment mechanism to each layer of the forest to reduce the computational complexity of the model. The deep neural forest was extended to the densely connected deep neural forest to improve the prediction results. The experiments on RNA-seq gene expression data showed that LACFNForest has better performance in the classification of cancer subtypes compared to the conventional methods. CONCLUSION: The LACFNForest model effectively improves the accuracy of cancer subtype classification with good robustness. It provides a new approach for the ensemble learning of classifiers in terms of structural design.


Assuntos
Neoplasias , Expressão Gênica , Humanos , Aprendizagem , Neoplasias/genética , RNA-Seq , Tamanho da Amostra
4.
Artigo em Inglês | MEDLINE | ID: mdl-34633806

RESUMO

A catalyst with high-entropy oxide (HEO)-stabilized single-atom Pt can afford low-temperature activity for catalytic oxidation and remarkable durability even under harsh conditions. However, HEO is easy to harden during sintering, which results in a few defective sites for anchoring single-atom metals. Herein, we present a sol-gel-assisted mechanical milling strategy to achieve a single-atom catalyst of Pt-HEO/Al2O3. The strong interaction between HEO and Al2O3 effectively inhibits the growth of HEO microparticles, which leads to generation of more surface defects because of the nanoscale effect. Meanwhile, another strong interaction between Pt and HEO stabilizes single-atom Pt on HEO. Temperature-programmed techniques further verify that the reactivity of surface lattice oxygen species is enhanced because of the Pt-O-M bonds on the surface of HEO. Unlike conventional single-atom Pt catalysts, Pt-HEO/Al2O3 as a heterogeneous catalyst not only exhibits superior stability against hydrothermal aging but also presents long-term reaction stability for CO catalytic oxidation, which exceeds 540 h. The present work opens a new door for rational design of hydrothermally stable single-atom Pt catalysts, which are highly promising in practical applications.

5.
Adv Mater ; : e2104246, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34608672

RESUMO

Despite the high theoretical capacity of lithium-sulfur (Li-S) batteries, a high cell-level energy density and a long cycling life are barely achieved, mainly due to the large electrolyte-to-sulfur ratio, polysulfide (PS) shuttle causing the loss of active sulfur, and the formation of passivation layers on the Li anode. To raise the energy density, holding PS in the cathode has been the most popular approach. Still, it has failed, particularly, when the sulfur loading is high enough to have energy densities similar to those of commercial Li-ion batteries. Here, a practical approach of achieving high "cell-level" energy densities is attempted using lithium PS (LPS)-containing electrolytes instead of a pure electrolyte, reducing the electrolyte-to-sulfur ratio and PS diffusion out of the cathode due to concentration differences. Meanwhile, the persistent problems including PS passivation and Li dendrites are suppressed using Li2 S-phobic artificial solid-electrolyte interphase (A-SEI) layers on Li metal. The synergistic effects from the LPS additives and A-SEI result in a superior cell-level volumetric energy density of 650 Wh L-1 as well as large cumulative energy densities considering cycling life. This approach provides an important stepping stone to realize commercial Li-S batteries rivaling the current Li-ion batteries.

6.
Artigo em Inglês | MEDLINE | ID: mdl-34623283

RESUMO

Network embedding is to learn low-dimensional representations of nodes while preserving necessary information for network analysis tasks. Though representations preserving both structure and attribute features have achieved in many real-world applications, learning these representations for networks with attribute information is difficult due to the heterogeneity between structure and attribute information. Many existing methods have been proposed to preserve explicit proximities between nodes, with optimization limited to node pairs with large structure and attribute proximities, which may lead to overfitting. To address the above problems, we adopt an attribute augmented network to represent attribute and structure information in a unified framework. Specifically, we study the problem of attribute augmented network embedding that exploits the strength of generative adversarial nets (ANGANs) in capturing the latent distribution of data to learn robust and informative representations of nodes. The ANGAN method obtains the low-dimensional representations of nodes through adversarial learning between the generative and discriminative models. The generative model approximates the underlying connectivity and attributes distributions of nodes by using the distributions generated from the learned representations. It is implemented by utilizing the properties of the attribute augmented network to improve the traditional Skip-gram model. The discriminative model is designed as a binary classifier to distinguish the truly connected node pairs from the generated ones. The pre-training algorithm and the teacher forcing approach are adopted to improve training efficiency and stability. Empirical results show that ANGAN generally outperforms state-of-the-art methods in various real-world applications, which demonstrates the effectiveness and generality of our method.

7.
Crit Rev Food Sci Nutr ; : 1-34, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34637646

RESUMO

The numerous health benefits of pectins justify their inclusion in human diets and biomedical products. This review provides an overview of pectin extraction and modification methods, their physico-chemical characteristics, health-promoting properties, and pharmaceutical/biomedical applications. Pectins, as readily available and versatile biomolecules, can be tailored to possess specific functionalities for food, pharmaceutical and biomedical applications, through judicious selection of appropriate extraction and modification technologies/processes based on green chemistry principles. Pectin's structural and physicochemical characteristics dictate their effects on digestion and bioavailability of nutrients, as well as health-promoting properties including anticancer, immunomodulatory, anti-inflammatory, intestinal microflora-regulating, immune barrier-strengthening, hypercholesterolemia-/arteriosclerosis-preventing, anti-diabetic, anti-obesity, antitussive, analgesic, anticoagulant, and wound healing effects. HG, RG-I, RG-II, molecular weight, side chain pattern, and degrees of methylation, acetylation, amidation and branching are critical structural elements responsible for optimizing these health benefits. The physicochemical characteristics, health functionalities, biocompatibility and biodegradability of pectins enable the construction of pectin-based composites with distinct properties for targeted applications in bioactive/drug delivery, edible films/coatings, nano-/micro-encapsulation, wound dressings and biological tissue engineering. Achieving beneficial synergies among the green extraction and modification processes during pectin production, and between pectin and other composite components in biomedical products, should be key foci for future research.

8.
Chem Res Chin Univ ; : 1-7, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34511841

RESUMO

Extracorporeal membrane oxygenation(ECMO) has emerged as a viable treatment in severe cases of acute respiratory distress syndrome, acute respiratory failure, and adult respiratory distress syndrome. However, thromboembolic events stemming from the use of ECMO devices results in significant morbidity and mortality rates; the inner surface of the ECMO tubing comes into contact with the blood and can readily initiate coagulation. In addition, the tubing needs to be continually replaced due to thromboses on the inner tube wall, which not only increases the risk of infection but also the economic burden. Despite considerable effort, a surface modification strategy that effectively addresses these challenges has not yet been realized. In this study, we developed an integrated hollow core-shell-shell hydrogel tube of gelatin/alginate/acrylamide-bacterial nanocellulose(GAA) that meets the anticoagulant requirements for the inner tubing layer as well as the highly elastic soft material needed for the outer layer. Using static blood from healthy volunteers, we confirmed that the platelets or coagulation is not stimulated by the GAA tubing. Importantly, experiments with dynamic blood also demonstrated that the inner layer of the tubing does not elicit blood clotting. The one-pot-synthesized process may provide guidance for the design of anticoagulation tubes used clinically. Electronic Supplementary Material: Supplementary material is available in the online version of this article at 10.1007/s40242-021-1267-3.

9.
Chem Asian J ; 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34473912

RESUMO

Reactions of N,P-Ligands as Ph2 P(o-NMe2 C6 H4 ) (1 L), 2,6-iPr2 C6 H3 NHC(Ph)=NC6 H4 (o-PPh2 ) (2 L), and Ph2 PN(R)PPh2 (R=iPr (3 L), cyclo-C6 H11 (4 L), tBu (5 L), CH2 C4 H7 O (6 L)) each with dicobalt octacarbonyl produced complexes [1 LCo(CO)3 ]2 (1), [2 LCo(CO)(µ-CO)2 Co(CO)3 ] (2), [3 LCo(CO)3 ]+ [Co(CO)4 ]- (3), [3 LCo(CO)2 ]2 (4), [4 LCo(CO)2 ]2 (5), [5 LCo(CO)2 ]+ [Co(CO)4 ]- (6), and [6 LCo(CO)2 ]+ [Co(CO)4 ]- (7). Complexes 1-7 have all been structurally characterized by X-ray crystallography, IR and NMR spectroscopies, and elemental analysis. Catalytic tests on transformation of ethylene oxide (EO), CO and MeOH into methyl 3-hydroxypropionate (3-HMP) indicate that complexes 1-7 are active, where ion-pair complexes 3 and 6-7 behave more excellently (by achieving 88.4-93.6% 3-HMP yields) than the neutral species 1-2 and 4-5 (35.0-46.5% 3-HMP yields) when the reactions are all operated at 2 MPa CO pressure and 50 °C in MeOH solvent. Density functional theory (DFT) study by selecting 3 as a model suggests a cooperative catalytic reaction mechanism by [Co(CO)4 ]- and its counter cation [3 LCo(CO)3 ]+ . The cobalt-homonuclear ion-pair catalyzed hydroalkoxycarbonylation of EO is present herein.

10.
Commun Biol ; 4(1): 1034, 2021 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-34465887

RESUMO

COVID-19 has caused numerous infections with diverse clinical symptoms. To identify human genetic variants contributing to the clinical development of COVID-19, we genotyped 1457 (598/859 with severe/mild symptoms) and sequenced 1141 (severe/mild: 474/667) patients of Chinese ancestry. We further incorporated 1401 genotyped and 948 sequenced ancestry-matched population controls, and tested genome-wide association on 1072 severe cases versus 3875 mild or population controls, followed by trans-ethnic meta-analysis with summary statistics of 3199 hospitalized cases and 897,488 population controls from the COVID-19 Host Genetics Initiative. We identified three significant signals outside the well-established 3p21.31 locus: an intronic variant in FOXP4-AS1 (rs1853837, odds ratio OR = 1.28, P = 2.51 × 10-10, allele frequencies in Chinese/European AF = 0.345/0.105), a frameshift insertion in ABO (rs8176719, OR = 1.19, P = 8.98 × 10-9, AF = 0.422/0.395) and a Chinese-specific intronic variant in MEF2B (rs74490654, OR = 8.73, P = 1.22 × 10-8, AF = 0.004/0). These findings highlight an important role of the adaptive immunity and the ABO blood-group system in protection from developing severe COVID-19.


Assuntos
COVID-19/etnologia , COVID-19/genética , Grupos Étnicos/genética , Estudo de Associação Genômica Ampla , Predisposição Genética para Doença/genética , Humanos , Íntrons/genética , Polimorfismo de Nucleotídeo Único
11.
Int J Artif Organs ; : 3913988211041636, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34474617

RESUMO

An accurate representation of the flow field in blood pumps is important for the design and optimization of blood pumps. The primary turbulence modeling methods applied to blood pumps have been the Reynolds-averaged Navier-Stokes (RANS) or URANS (unsteady RANS) method. Large eddy simulation (LES) method has been introduced to simulate blood pumps. Nonetheless, LES has not been widely used to assist in the design and optimization of blood pumps to date due to its formidable computational cost. The purpose of this study is to explore the potential of the LES technique as a fast and accurate engineering approach for the simulation of rotary blood pumps. The performance of "Light LES" (using the same time and spatial resolutions as the URANS) and LES in two rotary blood pumps was evaluated by comparing the results with the URANS and extensive experimental results. This study showed that the results of both "Light LES" and LES are superior to URANS, in terms of both performance curves and key flow features. URANS could not predict the flow separation and recirculation in diffusers for both pumps. In contrast, LES is superior to URANS in capturing these flows, performing well for both design and off-design conditions. The differences between the "Light LES" and LES results were relatively small. This study shows that with less computational cost than URANS, "Light LES" can be considered as a cost-effective engineering approach to assist in the design and optimization of rotary blood pumps.

12.
Med Biol Eng Comput ; 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34528164

RESUMO

Divergence-free smoothing with wall treatment (DFSwt) method is proposed for processing with four-dimensional (4D) flow magnetic resonance imaging (MRI) data of blood flows to enhance the quality of flow field with physical constraints. The new method satisfies the no-slip wall boundary condition and applies wall function of velocity profile for better estimating the velocity gradient in the near-wall region, and consequently improved wall shear stress (WSS) calculation against the issue of coarse resolution of 4D flow MRI. In the first testing case, blood flow field obtained from 4D flow MRI is well smoothed by DFSwt method. A great consistency is observed between the post-processed 4D flow MRI data and the computational fluid dynamics (CFD) data in the interested velocity field. WSS has an apparent improvement due to the proposed near-wall treatment with special wall function comparing to the result from original 4D flow MRI data or the DFS-processed data with no wall function. The other five cases also show the same performance that smoothed velocity field and improved WSS estimation are achieved on 4D flow MRI data optimized by DFSwt. The improvements will benefit the study of hemodynamics regarding the determination of location or the potential possibility of lesions.

13.
Health Qual Life Outcomes ; 19(1): 213, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34488798

RESUMO

BACKGROUND: Although physical activity (PA) and sedentary time in cancer survivors (CSs) were associated with health-related quality of life (HRQOL), it was not clear whether their associations were similar among CSs with different number of comorbid chronic diseases (CCDs). This study aimed to investigate the associations between PA, sedentary time and HRQOL in CSs with different number of CCDs. METHODS: A cross-sectional study was conducted among 1546 CSs between June and September 2018 in Shanghai, China. Data were collected with a self-reported questionnaire including sociodemographic characteristics, CCDs, PA, sedentary time and HRQOL. International Physical Activity Questionnaire and Cancer Quality of Life Questionnaire-Core30 were respectively used to measure PA and HRQOL of CSs. Associations of PA and sedentary time with HRQOL among CSs with different number of CCDs were evaluated by using logistic regression, adjusted for confounding factors. RESULTS: About seventy-five percent CSs had at least one CCD. Approximately three fifths CSs had high PA level and < 4 h/day sedentary time. Moderate PA level and high PA level were shown to be associated with better HRQOL among all participants. In CSs with ≤ 2 CCDs, high PA level was significantly associated with higher scores of physical function and lower scores of nausea and vomiting, appetite loss. However, there was a positive association between high PA level and constipation score among CSs with ≥ 3 CCDs. CSs with shorter sedentary time had better HRQOL in those with CCDs. CONCLUSIONS: High PA level and long sedentary time have significant association with worse HRQOL of CSs with ≥ 3 CCDs, while high PA level is positively associated with HRQOL in CSs with ≤ 2 CCDs. Our findings may support further studies of the causal association between PA, sedentary times and HRQOL to provide targeted proposal to improve the HRQOL of CSs according to their number of CCDs.


Assuntos
Sobreviventes de Câncer/psicologia , Exercício Físico/fisiologia , Neoplasias/mortalidade , Qualidade de Vida/psicologia , Comportamento Sedentário , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Exercício Físico/psicologia , Feminino , Comportamentos Relacionados com a Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/terapia , Inquéritos e Questionários , Terapêutica
14.
Neurol Res ; : 1-7, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34581255

RESUMO

OBJECTIVE: To evaluate the clinical efficacy of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs) in the treatment of spinal tuberculosis in rabbits. METHODS: 35 spinal tuberculosis rabbit models were grouped into three groups, including 14 in group A and group B respectively and 7 in group C.All rabbits in group A were treated by INH-RFP-BSA-NPs's injection and in group B were treated with classic dosage form of INH and RFP, while in group C normal saline was given as the blank control. After intervention, the body weighing and CT scan, as well as concentration's measurement of INH and RFP in blood and tissues, were performed in all rabbits at the time of the 6thweek and 12th week, respectively. RESULTS: In group A, rabbits' weight increased by 0.44 kg and 0.27 kg within 6 weeks and 12 weeks' treatment respectively. The bactericidal concentrations of 1.64 µg•g-1 for INH and 21.36 µg•g-1 for RFP were measured in focus vertebral body 6 weeks post-injection and six weeks later the concentrations of INH and RFP in vertebral body still maintained at the level of 1.96 µg•g-1 and 22.35 µg•g-1respectively. After 12 weeks therapy, CT-scanned showed all the necrotic tissue was replaced by normal bone tissue. In group B, all rabbits had no significant increment of body weight and 4 rabbits had paralysis of hind leg. The concentrations of INH and RFP in vertebral body and focus were much lower than group A. CT-scanned showed the focus vertebral body was only partially repaired after 12 weeks' therapy. CONCLUSION: The INH-RFP-BSA-NPs has the characteristics of sustained release in vivo and target biodistribution in focus vertebral body. Its therapeutic effect in rabbit spinal tuberculosis is much better than common INH and RFP.

16.
J Phys Chem Lett ; 12(38): 9205-9212, 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34529435

RESUMO

Triplet exciton formation is essential for photosensitization-based photochemistry and photobiology. The heavy atom effect (HAE), in the form of either external or internal mode, is a basic mechanism for increasing the triplet exciton yield of photosensitizers. Herein, we report a new HAE mode by noncovalent cohosting of heavy atoms and photosensitizers in a double-stranded DNA (dsDNA) matrix. With dsDNA bearing several thymine (T) or cytosine (C) mismatches, heavy atoms (e.g., Hg2+ or Ag+) and dsDNA-staining dyes (photosensitizers) were spatially adjoined in close proximity, thus resulting in enhanced phosphorescence and 1O2 generation from the photosensitizers. The dsDNA-hosted HAE provides highly selective recognition for the heavy atoms, which is not applicable in either the external or the internal mode. Considering the simpleness and efficiency of the spatially adjoined HAE, as well as the functionality of DNA, the proposed HAE mode is appealing for various singlet oxygen- and phosphorescence-related applications.

17.
J Prosthodont Res ; 2021 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-34588401

RESUMO

PURPOSE: To evaluate the performance of polyetheretherketone (PEEK) versus titanium computer-aided designed and manufactured (CAD-CAM) framework for implant-supported fixed complete dentures (ISFCDs) with a follow-up for a duration of up to 5 years. METHODS: Consecutively edentulous patients who underwent ISFCDs with a PEEK framework or titanium framework at one dental specialist center were included in this retrospective study. Implant/prosthesis survival rates, mechanical/biological complications, and bone and soft tissue parameters were analyzed. Overall survival was analyzed using Kaplan-Meier survival curves and the log-rank test. RESULTS: Sixty ISFCDs (29 PEEK, 31 titanium) performed on 43 edentulous patients (331 implants) were included. An implant survival rate of 100% was obtained. There was no significant difference in the cumulative prosthesis survival rate between the PEEK (93.1%) and titanium groups (93.5%). The most common mechanical complications were fracture of the artificial veneer in both the PEEK (13.8%) and titanium (16.7%) groups. Bruxers had a higher prevalence of mechanical complications than non-bruxers (p<0.05). The biological complications included bleeding upon probing (13.8% for the PEEK group; 16.1% for the titanium group), soft tissue inflammation (3.4% for the PEEK group; 3.2% for the titanium group), and temporomandibular disorders (6.5% for the titanium group). The vertical bone loss was significantly lower in the PEEK group (0.70 mm) than in the titanium group (0.96 mm). Smokers had a significantly higher prevalence of biological complications than non-smokers. CONCLUSIONS: Within the limitations of this study, ISFCDs with PEEK frameworks can provide good prognosis for edentulous patients, still requiring longer-term validation.

18.
Anal Chem ; 93(38): 13014-13021, 2021 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-34524814

RESUMO

Rheumatoid arthritis (RA) is a common chronic autoimmune inflammatory disease, and its etiology is closely related to the overproduction of hypochlorous acid (HClO). However, early detection of RA using an activatable near-infrared-II (NIR-II, 1000-1700 nm) fluorescent probe remains challenging. Herein, we first report an "OFF-ON" NIR-II fluorescent probe named PTA (phenothiazine triphenylamine) for imaging HClO in deep-seated early RA. Electron-rich phenothiazine in the core of PTA was utilized as both an HClO-recognition moiety and a precursor of electron acceptors, displaying a typical donor-acceptor-donor structure with excellent NIR-II emission at 936/1237 nm once reacted with HClO. The probe PTA exhibited good water solubility, high photostability, and rapid response capability toward HClO within 30 s. Moreover, it was able to sensitively and specifically detect exogenous and endogenous HClO in living cells in both visible and NIR-II windows. Notably, PTA enabled the sensitive and rapid visualization of HClO generation in an inflammatory RA mouse model, showing a 4.3-fold higher NIR-II fluorescence intensity than that in normal hindlimb joints. These results demonstrate that PTA holds great promise as a robust platform for diagnosis of HOCl-mediated inflammatory disorders.


Assuntos
Artrite Reumatoide , Ácido Hipocloroso , Animais , Artrite Reumatoide/diagnóstico por imagem , Modelos Animais de Doenças , Corantes Fluorescentes , Camundongos
19.
Clin Transl Med ; 11(9): e525, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34586737

RESUMO

N6 -methyladenosine (m6 A), the newest and most prevalent layer of internal epigenetic modification in eukaryotic mRNA, has been demonstrated to play a critical role in cancer biology. Increasing evidence has highlighted that the interaction between cancer stem cells (CSCs) and the tumor immune microenvironment (TIME) is the root cause of tumorigenesis, metastasis, therapy resistance, and recurrence. In recent studies, the m6 A modification has been tightly linked to this CSC-TIME interplay, participating in the regulation of CSCs and TIME remolding. Interestingly, the m6 A modification has also been identified as a novel decisive factor in the efficacy of immunotherapies-particularly anti-PD-1/PD-L1 monotherapies-by changing the plasticity of the TIME. Given the functional importance of the m6 A modification in the crosstalk between CSCs and the TIME, targeting m6 A regulators will open new avenues to overcome therapeutic resistance, especially for immune checkpoint-based immunotherapy. In the present review, we summarize the current landscape of m6 A modifications in CSCs and the TIME, and also prospect the underling role of m6 A modifications at the crossroads of CSCs and the TIME for the first time. Additionally, to provide the possibility of modulating m6 A modifications as an emerging therapeutic strategy, we also explore the burgeoning inhibitors and technologies targeting m6 A regulators. Lastly, considering recent advances in m6 A-seq technologies and cancer drug development, we propose the future directions of m6 A modification in clinical applications, which may not only help to improve individualized monitoring and therapy but also provide enhanced and durable responses in patients with insensitive tumors.

20.
Sci Rep ; 11(1): 17503, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34471173

RESUMO

Soil properties, such as organic carbon, pH and clay content, are critical indicators of ecosystem function. Visible-near infrared (vis-NIR) reflectance spectroscopy has been widely used to cost-efficiently estimate such soil properties. Multivariate modelling, such as partial least squares regression (PLSR), and machine learning are the most common methods for modelling soil properties with spectra. Often, such models do not account for the multiresolution information presented in the vis-NIR signal, or the spatial variation in the data. To address these potential shortcomings, we used wavelets to decompose the vis-NIR spectra of 226 soils from agricultural and forested regions in south-western Western Australia and developed a wavelet geographically weighted regression (WGWR) for estimating soil organic carbon content, clay content and pH. To evaluate the WGWR models, we compared them to linear models derived with multiresolution data from a wavelet decomposition (WLR) and PLSR without multiresolution information. Overall, validation of the WGWR models produced more accurate estimates of the soil properties than WLR and PLSR. Around 3.5-49.1% of the improvement in the estimates was due to the multiresolution analysis and 1.0-5.2% due to the integration of spatial information in the modelling. The WGWR improves the modelling of soil properties with spectra.

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