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1.
J Hazard Mater ; 422: 126900, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34418829

RESUMO

Carbon sources have been reported to determine the bio-demulsifying performance and mechanisms. However, the genetic regulation of carbon sources-mediated bio-demulsification remains unclear. Here, the effects of ß-oxidation, stress response, and nitrate metabolism on the demulsification of alkaline-surfactant-polymer flooding produced water by Gordonia sp. TD-4 were investigated. The results showed that competitive adsorption-derived demulsification was mediated by oil-soluble carbon sources (paraffin). Surface-active lipopeptides responsible for competitive adsorption-derived demulsification could be biosynthesized by the nonribosomal peptide synthetases and polyketide synthases using oil-soluble carbon sources. Bio-flocculation-derived demulsification was mediated by water-soluble carbon sources. Water-soluble carbon sources (sodium acetate and glucose) mediated the process of the dissimilatory reduction of nitrate to ammonia, which resulted in the variable accumulation of nitrite. The accumulated nitrite (>180 mg-N/L) stimulated stress response and induced the upregulation of chaperone-associated genes. The upregulation of chaperonins increased the cell surface hydrophobicity and the cation-dependent bio-flocculating performance, which were responsible for bio-flocculation-derived demulsification. The ß-oxidation of fatty acids significantly affected both competitive adsorption-derived demulsification and bio-flocculation-derived demulsification. This study illustrates the synergistic effects of nitrogen sources and carbon sources on the regulation of bio-demulsifying mechanisms of TD-4 and identifies two key functional gene modules responsible for the regulation of bio-demulsifying mechanisms.

2.
Biosens Bioelectron ; 195: 113663, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34610534

RESUMO

Tuberculosis (TB) remains the high-risk infectious pathogen that caused global pandemic and high mortality, particularly in the areas lack in health resources. Clinical TB screening and diagnosis are so far mainly conducted on limited types of commercial platforms, which are expensive and require skilled personnel. In this work, we introduced a low-cost and portable finger-driven microfluidic chip (named Fd-MC) based on recombinase polymerase amplification (RPA) for rapid on-site detection of TB. After injection of the pre-treated sample solution, the pre-packaged buffer was driven by the pressure generated by the finger-actuated operation to accomplish sequential processes of diagnosis in a fully isolated microchannel. An in-situ fluorescence strategy based on FAM-probe was implemented for on-chip results read-out though a hand-held UV lamp. Hence, the Fd-MC proved unique advantageous for avoiding the risk of infection or environmental contamination. In addition, the Fd-MC was designed for multiplexed detection, which is able to not only identify TB/non-TB infection, but also differentiate between human Mycobacterium tuberculosis and Mycobacterium bovis. The platform was verified in 37 clinical samples, statistically with 100% specificity and 95.2% sensitivity as compared to commercial real-time RPA. Overall, the proposed platform eliminates the need on external pumps and skilled personnel, holding promise to POC testing in the resource-limited area.


Assuntos
Técnicas Biossensoriais , Mycobacterium bovis , Mycobacterium tuberculosis , Tuberculose , Humanos , Mycobacterium tuberculosis/genética , Sistemas Automatizados de Assistência Junto ao Leito , Tuberculose/diagnóstico
3.
Chem Biodivers ; : e2100687, 2021 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-34726832

RESUMO

Toxoplasmosis post serious threaten to human health, leading to severely eye and brain disease, especially for immunocompromised patients and pregnant women. The multiple side effects and long dosing period of current main treatment regiments calls for high effective and low toxicity anti-toxoplasmosis drugs. Herein, we report our efforts to synthesize a series of 2-(piperazin-1-yl)quinazolin-4(3H)-one derivatives and investigate their activity against Toxoplasma gondii tachyzoites in vitro based on cell phenotype screening. Among the 26 compounds, 8w and 8x with diaryl ether moiety at the side chain of piperazine exhibited good efficacy to inhibit T. gondii, with IC50 values of 4 µM and 3 µM, respectively. Structure-activity relationship (SAR) studies implies that hydrophobic aryl at the side chain would be preferred for improvement of activity. Molecular docking study reveals these two compounds appeared high affinity to TgCDPK1 by interaction with the hydrophobic pocket of ATP-binding cleft.

4.
Comput Methods Programs Biomed ; 212: 106480, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34736168

RESUMO

BACKGROUND AND OBJECTIVE: High-dimensional data generally contains more accurate information for medical image, e.g., computerized tomography (CT) data can depict the three dimensional structure of organs more precisely. However, the data in high-dimension often needs enormous computation and has high memory requirements in the deep learning convolution networks, while dimensional reduction usually leads to performance degradation. METHODS: In this paper, a two-dimensional deep learning segmentation network was proposed for medical volume data based on multi-pinacoidal plane fusion to cover more information under the control of computation.This approach has conducive compatibility while using the model proposed to extract the global information between different inputs layers. RESULTS: Our approach has worked in different backbone network. Using the approach, DeepUnet's Dice coefficient (Dice) and Positive Predictive Value (PPV) are 0.883 and 0.982 showing the satisfied progress. Various backbones can enjoy the profit of the method. CONCLUSIONS: Through the comparison of different backbones, it can be found that the proposed network with multi-pinacoidal plane fusion can achieve better results both quantitively and qualitatively.


Assuntos
Aprendizado Profundo , Processamento de Imagem Assistida por Computador , Tomografia Computadorizada por Raios X
5.
Oral Dis ; 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34739175

RESUMO

The folate-mediated one-carbon metabolism pathway is thought to play an important role in the etiology of non-syndromic oral clefts (NSOFC), although none of the genes in this pathway has shown significant signals in genome-wide association studies (GWAS). Recent evidence indicated that enhanced understanding could be gained by aggregating multiple SNPs effect simultaneously into polygenic risk score (PRS) to assess its association with disease risks. This study is aimed to assess the association between the genetic effect of folate-mediated one-carbon metabolism pathway and NSOFC risks using PRS based on a case-parent trio design. A total of 297 SNPs mapped from 18 genes in the folate-mediated one-carbon metabolism pathway were aggregated from a GWAS of 2458 case-parent trios recruited from an international consortium. We found a PRS based on the folate-mediated one-carbon metabolism pathway was significant among all NSOFC trios (OR = 1.95, 95% CI: 1.66-2.28, p = 2.39 × 10-16 ), as well as two major subtypes, non-syndromic cleft lip with or without cleft palate (NSCL/P) trios (OR = 1.71, 95% CI: 1.50-1.96, p = 7.66 × 10-15 ) and non-syndromic cleft palate only (NSCPO) trios (OR = 1.51, 95% CI: 1.36-1.68, p = 2.1 × 10-14 ). Similar results were also observed in further subgroup analyses stratified into Asian and European trios. The averaged PRS of the folate-mediated one-carbon metabolism pathway varied between the NSOFC case group and its comparison group (p < 0.05) with higher average PRS in the cases. Moreover, the top 5% pathway PRS group had 2.25 (95% CI: 1.85-2.73) times increased NSOFC risk, also 3.09 (95% CI: 2.50-3.81) and 2.06 (95% CI: 1.39-3.02) times increased risk of NSCL/P and NSCPO compared to the remainder of the distribution. The results of our study confirmed the folate-mediated one-carbon metabolism pathway was important in controlling risk to NSOFC and this study enhanced evidence towards understanding the genetic risks of NSOFC.

6.
J Int Med Res ; 49(11): 3000605211055410, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34772309

RESUMO

We report a case of interdigitating dendritic cell sarcoma (IDCS) originating from the adrenal gland. A 57-year-old middle-aged woman with no previous history of malignancy came to our hospital after color Doppler ultrasound revealed a right adrenal mass. An abdominal computed tomography scan also showed an adrenal mass. Postoperative pathology confirmed the diagnosis of IDCS. After complete surgical removal of the adrenal tumor, the patient has been disease-free for 1 year. IDCS may have a good prognosis after surgical resection. To our knowledge, this is only the second reported case of IDCS in the adrenal region.


Assuntos
Sarcoma de Células Dendríticas Interdigitantes , Linfoma não Hodgkin , Glândulas Suprarrenais/diagnóstico por imagem , Glândulas Suprarrenais/cirurgia , Feminino , Humanos , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X
7.
J Int Med Res ; 49(11): 3000605211058874, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34775845

RESUMO

OBJECTIVE: We performed a retrospective study to compare the accuracy of preoperative planning using three-dimensional AI-HIP software and traditional two-dimensional manual templating to predict the size and position of prostheses. The purpose of this study was to evaluate the accuracy of AI-HIP in preoperative planning for primary total hip arthroplasty. METHODS: In total, 316 hips treated from April 2019 to June 2020 were retrospectively reviewed. A typical preoperative planning process for patients was implemented to compare the accuracy of the two preoperative planning methods with respect to prosthetic size and position. Intraclass correlation coefficients (ICCs) were used to evaluate the homogeneity between the actual prosthetic size and position and the preoperative planning method. RESULTS: When AI-HIP software and manual templating were used for preoperative planning, the stem agreement was 87.7% and 58.9%, respectively, and the cup agreement was 94.0% and 65.2%, respectively. The results showed that when AI-HIP software was used, an extremely high level of consistency (ICC > 0.95) was achieved for the femoral stem size, cup size, and femoral osteotomy level (ICC = 0.972, 0.962, and 0.961, respectively). CONCLUSION: AI-HIP software showed excellent reliability for predicting the component size and implant position in primary total hip arthroplasty.


Assuntos
Artroplastia de Quadril , Prótese de Quadril , Inteligência Artificial , Humanos , Cuidados Pré-Operatórios , Reprodutibilidade dos Testes , Estudos Retrospectivos , Software
8.
Food Funct ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34788772

RESUMO

Lung cancer is a common cancer with high mortality worldwide, and non-small cell lung cancer (NSCLC) accounts for the majority. The clinical treatment effect of NSCLC is not ideal. The aim of this study was to investigate the inhibitory effect of sea cucumber peptide (SCP) on NSCLC and its mechanism. The results showed that SCP could effectively inhibit the proliferation, migration and invasion of A549 cells. In addition, SCP can also inhibit the formation of pleural effusion and tumor growth in lung cancer mice, reduce liver and kidney injury, increase the levels of IL-2 and IL-12, decrease the levels of IL-6 and TNF-α, and prolong the survival time of mice. The microRNA sequencing and immunohistochemistry of mouse tumors showed that the tumor suppressor gene TUSC2 targeted by miR-378a-5p was involved in the inhibition of tumor growth by SCP. This study provides an experimental basis for the further development of SCP as an anti-tumor nutritional supplement, and provides a new idea for exploring the molecular mechanism of food derived active peptides in anti-tumor applications.

9.
Front Microbiol ; 12: 732426, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34733250

RESUMO

Salmonella spp. is one of the most common foodborne disease-causing pathogens that can cause severe diseases in very low infectious doses. Rapid and sensitive detecting Salmonella spp. is advantageous to the control of its spread. In this study, a conserved short fragment of the Salmonella invA gene was selected and used to design primers and specific crRNA (CRISPR RNA) for establishing a one-tube and two-step reaction system for Salmonella spp. detection, by combining recombinase polymerase amplification (RPA) with CRISPR-Cas13a (Clustered Regularly Interspaced Short Palindromic Repeats associated protein 13a) cleavage. The established one-tube RPA-Cas13a method can complete the detection within 20 min and the two-step RPA-Cas13a method detection time within 45 min. The designed primers were highly specific to Salmonella spp. and had no cross-reaction with the other nine diarrheal bacteria. The one-tube RPA-Cas13a could detect the Salmonella genome with the limit of 102 copies, which was the same as real-time polymerase chain reaction (PCR), but less sensitive than two-step RPA-Cas13a (100 copies). The detection results of one-tube or two-step RPA-Cas13a and real-time PCR were highly consistent in clinical samples. One-tube RPA-Cas13a developed in this study provides a simple, rapid, and specific detection method for Salmonella spp. While two-step assay was more sensitive and suitable for samples at low abundance.

10.
Analyst ; 146(23): 7257-7264, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34734932

RESUMO

Here, an Au-Cu dual-electrode tip was designed to monitor the effect of Cu2+ on the membrane permeability of a single living cell in situ using scanning electrochemical microscopy. The probe approach curves (PACs) were obtained using potassium ferricyanide as a redox mediator. Meanwhile, according to the simulation, theoretical PACs could be acquired. Thus, the cell membrane permeability coefficient (Pm) values were obtained by overlapping the experimental PACs with the theoretical values. Cu2+ was directly generated by electrolyzing the Cu electrode of the dual-electrode tip to investigate its effect on the cell membrane permeability in situ. This work has potential value to improve the understanding of the mechanism of acute heavy metal damage on the cell membrane and will also help clarify the role of heavy metal ions in physiological or pathological processes.


Assuntos
Microscopia Eletroquímica de Varredura , Permeabilidade da Membrana Celular , Eletrodos , Oxirredução , Permeabilidade
11.
Front Cell Infect Microbiol ; 11: 724401, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34796123

RESUMO

Enterotoxigenic Escherichia coli (ETEC) is a common enteric pathogen that causes diarrhoea in humans and animals. Lactobacillus rhamnosus LB1 (formerly named Lactobacillus zeae LB1) has been shown to reduce ETEC infection to Caenorhabditis elegans and Salmonella burden in pigs. This study was to evaluate the effect of L. rhamnosus LB1 on the gut health of lactating piglets that were challenged with ETEC. Six-four piglets at 7 days of age were equally assigned into 8 groups (8 piglets per group): 1) control group (basal diet, phosphate buffer saline); 2) CT group (basal diet + 40 mg/kg colistin); 3) LL group (basal diet + 1 × 107 CFU/pig/day LB1); 4) HL group (basal diet + 1 × 108 CFU/pig/day LB1); 5) ETEC group: (basal diet + ETEC challenged); 6) CT + ETEC group (basal diet + CT + ETEC); 7) LL + ETEC group (basal diet + 1 × 107 CFU/pig/day LB1 + ETEC); 8) HL + ETEC group (basal diet + 1 × 108 CFU/pig/day LB1 + ETEC). The trial lasted ten days including 3 days of adaptation. Several significant interactions were found on blood parameters, intestinal morphology, gene, and protein expression. ETEC infection disrupted the cell structure and biochemical indicators of blood, undermined the integrity of the intestinal tract, and induced oxidative stress, diarrhoea, intestinal damage, and death of piglets. The supplementation of L. rhamnosus LB1 alleviated ETEC's adverse effects by reducing pig diarrhoea, oxidative stress, and death, modulating cell structure and biochemical indicators of blood, improving the capacity of immunity and anti-oxidation stress of pigs, and restoring their intestinal integrity. At the molecular level, the beneficial effects of L. rhamnosus LB1 appeared to be mediated by regulating functional related proteins (including HSP70, Caspase-3, NLRP3, AQP3, and AQP4) and genes (including RPL4, IL-8, HP, HSP70, Mx1, Mx2, S100A12, Nrf2, GPX2 and ARG1). These results suggest that dietary supplementation of L. rhamnosus LB1 improved the intestinal functions and health of piglets.


Assuntos
Escherichia coli Enterotoxigênica , Infecções por Escherichia coli , Lactobacillus rhamnosus , Doenças dos Suínos , Animais , Caenorhabditis elegans , Infecções por Escherichia coli/prevenção & controle , Infecções por Escherichia coli/veterinária , Feminino , Imunidade , Lactação , Lactobacillus , Estresse Oxidativo , Suínos
12.
Anal Chem ; 93(47): 15794-15801, 2021 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-34779626

RESUMO

Rational design of electrochemiluminescence (ECL) reagents is essential for the development of ECL biosensors with superior performances. In this work, the assembly of tris(1,10-phenanthroline)ruthenium(II) [Ru(phen)32+] and tetrahedral chalcogenide nanoclusters of [Cd32S14(SC6H5)38]2- in the formation of complex nanoclusters (CdS-Ru) was developed, in which Ru(phen)32+ was uniformly encapsulated and dispersed at a molecular level in the chalcogenide nanocluster via multiple noncovalent interactions. It was observed that the promoted ECL emission was realized by the charge transfer between the tetrahedral CdS nanocluster and Ru(phen)32+ by the formation of the assembly complex, which was elucidated by cyclic voltammetry curves, ECL-potential curves, and in situ dynamic ECL spectra. Taking advantages of the facile charge transfer in the open framework CdS-Ru, a high ECL efficiency has been achieved with remarkable stability. Moreover, a solid-state ECL sensor based on the CdS-Ru modified electrode was fabricated for label-free detection of alkaline phosphatase (ALP) activity with a detection limit as low as 0.35 U/L and superior reproducibility. This solid-state ECL sensor also displayed favorable selectivity among various interferences and was applied for ALP activity analysis in human serum samples. These results implicated the potential applications of CdS-Ru for sensitive ECL analysis in complicated reaction systems and enlightened the rational design for self-enhanced and highly efficient ECL materials.

13.
Anal Chem ; 93(46): 15468-15473, 2021 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-34766749

RESUMO

A laser frequency-locked hollow waveguide (HWG) gas sensor is demonstrated for simultaneous measurements of three isotopologues (12CO2, 13CO2, and 18OC16O) using wavelength modulation spectroscopy with a 2.73 µm distributed feedback laser. The first harmonic (1f) signal at the sampling point where the peak of the second harmonic (2f) signal was located was employed as the locking point to lock the laser frequency to the transition center of 13CO2, while the absorption lines of 12CO2 and 18OC16O were being scanned. Continuous measurements of the three isotopologues of 4.7% CO2 samples over 103 min under free running and frequency locking conditions were performed. The measurement accuracy and precision of the three isotopologues achieved under the frequency locking condition were at least 3 times and 1.3 times better than those obtained under the free running condition, respectively. The Allan variance plot of the developed laser-locked HWG gas sensor shows a detection limit of 0.72‰ for both δ13C and δ18O under the frequency locking condition with a long stability time of 766 s. This study demonstrated the high potential of a novel human breath diagnostic sensor for medical diagnostic with high accuracy, precision, and sensitivity and without frequently repeated calibration.


Assuntos
Dióxido de Carbono , Lasers , Humanos , Análise Espectral
14.
Ann Palliat Med ; 10(10): 10986-10996, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34763461

RESUMO

BACKGROUND: A number of researches indicated preoperative functional exercise may improve the rehabilitation progress. This study aimed to investigate the effect of preoperative exercise intervention on rehabilitation before total knee arthroplasty (TKA) by literature retrieval and meta-analysis. METHODS: Embase, Cochrane library, PubMed, Ovid, and ClinicalTrials.gov were selected as the search database platforms. All published English articles related to preoperative exercise of TKA from January 2000 to January 2021 were searched with the following keyword strategy: ("preoperative functional exercise" or "prehabilitation exercise") AND ("TKA" or "total knee arthroplasty"). After literature screened according to the inclusion and exclusion criteria, the risk of bias and quality of the included articles were evaluated. RevMan 5.3.5 software was used for analysis to obtain the forest plot and funnel plot. RESULTS: A total of 911 articles were preliminarily searched in this study, and 12 were finally included for the quantitative analysis, comprising 889 patients; the result showed after intervention, the experimental group patients had wider ROM flexion than the control group patients [mean difference (MD) =4.28; 95% CI: 2.28 to 6.28; Z=4.19; P<0.0001]; the experimental group patients were with higher quadriceps strength value than the control group (MD =1.86, 95% CI: 0.58-3.15; Z=2.84, P=0.005); the WOMAC score (The Western Ontario and McMaster Universities Osteoarthritis Index), were higher for the experimental group patients (MD =-10.59; 95% CI: -11.88 to -9.29; Z=16.03; P<0.00001); and the standing and walking test index are higher (MD =-1.29, 95% CI: -1.90 to -0.67; Z=4.08; P<0.001); the quality of life score were higher too for the experimental group patients (MD =1.66; 95% CI: 1.13-2.20; Z=6.08; P<0.00001). DISCUSSION: Preoperative exercise intervention before TKA can improve knee flexion and flexibility, reduce inflammatory pain and stiffness, improve muscle strength, improve joint function, and thus improve the quality of life of patients.


Assuntos
Artroplastia do Joelho , Osteoartrite do Joelho , Humanos , Osteoartrite do Joelho/cirurgia , Exercício Pré-Operatório , Qualidade de Vida , Amplitude de Movimento Articular
15.
New Phytol ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34812496

RESUMO

Transcription activator-like (TAL) effectors are major virulence factors secreted by the type III secretion systems of Xanthomonas oryzae pv. oryzicola (Xoc) and X. oryzae pv. oryzae (Xoo), causing bacterial leaf streak and bacterial blight, respectively, in rice. However, the knowledge of Xoc TAL effector function in promoting bacterial virulence remains limited. Here, we isolated the highly virulent Xoc strain HGA4 from the outbreak region of Huanggang (Hubei, China), which contains four TAL effectors not found in the Chinese model strain RS105. Among these, Tal2b was selected for introduction into RS105, which resulted in a longer lesion length than that in the control. Tal2b directly binds to the promoter region of the gene and activates the expression of OsF3H03g , which encodes 2-oxoglutarate-dependent dioxygenase in rice. OsF3H03g negatively regulates salicylic acid (SA)-related defense by directly reducing SA, and it plays a positive role in susceptibility to both Xoc and Xoo in rice. OsF3H03g interacts with a uridine diphosphate-glycosyltransferase protein (OsUGT74H4), which positively regulates bacterial leaf streak susceptibility and may inactivate SA via glycosylation modification.

16.
Dalton Trans ; 50(45): 16473-16477, 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34730579

RESUMO

Reported here is an attractive pillar-layered metal chalcogenide open framework, in which [Sb2S5] building units act as pillars between [Mn5S12(N2H4)6]n layers. The obtained compound exhibits high stability in both acid and base media and good performance in the electrocatalytic oxygen reduction reaction (ORR).

17.
Cell Death Dis ; 12(12): 1108, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34839347

RESUMO

Abnormal lipid metabolism has been commonly observed in various human cancers, including colorectal cancer (CRC). The mitochondrial citrate carrier SLC25A1 (also known as mitochondrial citrate/isocitrate carrier, CIC), has been shown to play an important role in lipid metabolism regulation. Our bioinformatics analysis indicated that SLC25A1 was markedly upregulated in CRC. However, the role of SLC25A1 in the pathogenesis and aberrant lipid metabolism in CRC remain unexplored. Here, we found that SLC25A1 expression was significantly increased in tumor samples of CRC as compared with paired normal samples, which is associated with poor survival in patients with CRC. Knockdown of SLC25A1 significantly inhibited the growth of CRC cells by suppressing the progression of the G1/S cell cycle and inducing cell apoptosis both in vitro and in vivo, whereas SLC25A1 overexpression suppressed the malignant phenotype. Additionally, we demonstrated that SLC25A1 reprogrammed energy metabolism to promote CRC progression through two mechanisms. Under normal conditions, SLC25A1 increased de novo lipid synthesis to promote CRC growth. During metabolic stress, SLC25A1 increased oxidative phosphorylation (OXPHOS) to protect protects CRC cells from energy stress-induced cell apoptosis. Collectively, SLC25A1 plays a pivotal role in the promotion of CRC growth and survival by reprogramming energy metabolism. It could be exploited as a novel diagnostic marker and therapeutic target in CRC.

18.
Bioresour Technol ; : 126401, 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34822985

RESUMO

This study explored the regulation of photosystem and central carbon metabolism in cell growth and fucoxanthin accumulation of Isochrysis zhangjiangensis via transcriptome analysis, targeted metabolite measurements, and flux balance analysis. High light promoted biomass accumulation but dramatically decreased fucoxanthin productivity. It suppressed the active photosystem and reduced chlorophyll content, but improved metabolic flux of Calvin-Benson-Bassham and tricarboxylic acid cycle for massive biomass accumulation. The CO2 fixation was largely dependent on mitochondrial energy illustrated by the integrated metabolic tools. At a molecular level, glyceraldehyde-3-phosphate, acetyl-CoA, and pyruvate contents increased at exponential phase under high light, which tended to participate into fatty acid biosynthesis by the up-regulated ACCase. However, high light inhibited most genes involved in fucoxanthin biosynthesis and induced diadinoxanthin cycle to diatoxanthin form. Therefore, constant light at 100 µmol m-2 s-1 balancing biomass concentration and fucoxanthin content provided the highest fucoxanthin productivity at 3.06 mg L-1 d-1.

19.
J Colloid Interface Sci ; 608(Pt 2): 1999-2008, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34749148

RESUMO

HYPOTHESIS: The performance of a polymeric core-shell microreactor depends critically on (i) mass transfer, (ii) catalyzed chemical reaction, and (iii) deactivation within the nonuniform core-shell microstructure environment. As such, these three basic working principles control the active catalytic phase density in the reactor. THEORY: We present a high-fidelity, image-based nonequilibrium computational model to quantify and visualize the mass transport as well as the deactivation process of a core-shell polymeric microreactor. In stark contrast with other published works, our microstructure-based computer simulation can provide a single-particle visualization with a micrometer spatial accuracy. FINDINGS: We show how the interplay of kinetics and thermodynamics controls the product-induced deactivation process. The model predicts and visualizes the non-trivial, spatially resolved active catalyst phase patterns within a core-shell system. Moreover, we also show how the microstructure influences the formation of foulant within a core-shell structure; that is, begins from the core and grows radially onto the shell section. Our results suggest that the deactivation process is highly governed by the porosity/microstructure of the microreactor as well as the affinity of the products towards the solid phase of the reactor.

20.
Sensors (Basel) ; 21(22)2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34833574

RESUMO

Population based search techniques have been developed and applied to wide applications for their good performance, such as the optimization of the unmanned aerial vehicle (UAV) path planning problems. However, the search for optimal solutions for an optimization problem is usually expensive. For example, the UAV problem is a large-scale optimization problem with many constraints, which makes it hard to get exact solutions. Especially, it will be time-consuming when multiple UAV problems are waiting to be optimized at the same time. Evolutionary multi-task optimization (EMTO) studies the problem of utilizing the population-based characteristics of evolutionary computation techniques to optimize multiple optimization problems simultaneously, for the purpose of further improving the overall performance of resolving all these problems. EMTO has great potential in solving real-world problems more efficiently. Therefore, in this paper, we develop a novel EMTO algorithm using a classical PSO algorithm, in which the developed knowledge transfer strategy achieves knowledge transfer between task by synthesizing the transferred knowledges from a selected set of component tasks during the updating of the velocities of population. Two knowledge transfer strategies are developed along with two versions of the proposed algorithm. The proposed algorithm is compared with the multifactorial PSO algorithm, the SREMTO algorithm, the popular multifactorial evolutionary algorithm and a classical PSO algorithm on nine popular single-objective MTO problems and six five-task MTO problems, which demonstrates its superiority.

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