Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.605
Filtrar
1.
J Nanosci Nanotechnol ; 21(2): 935-942, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183427

RESUMO

In order to study Fe3O4-Polypyrrole (Fe3O4-PPy) core-shell nanocomposite in the diagnosis of tumor markers in the diseased gastric tissues of early cancer patients, a total of 160 cases of patients, who were confirmed as early gastric cancer by gastroscopy or postoperative pathology at a designated hospital of the study from December 2014 to December 2018, were selected as research objects and were divided into two groups of observation and control group with 80 cases in each group. For each patient in the two groups, two pieces of diseased gastric tissue were firstly taken through gastroscopy; then the observation group applied Fe3O4-PPy with the particle diameter of 150-350 nm as carrier to detect the contents of carbohydrate antigen 19-9 (CA19-9), alpha-fetoprotein (AFP), carbohydrate antigen 242 (CA242), carcinoembryonic antigen (CEA) and carbohydrate antigen 72-4 (CA72-4) in the diseased gastric tissue, while the control group directly detected the contents of corresponding tumor markers; after the detections, the sensitivity, specificity and diagnostic efficiency of each marker in the two groups of patients were calculated, and receiver operating characteristics (ROC) curves with the areas under the curves (AUC) were drawn to analyze the correlation between the level of Fe3O4-PPy and the detection efficiency of gastric cancer markers. The results show that the detection sensitivity (82.17%, 80.32%, 79.48%, 84.63%, and 85.66%) and specificity (76.75%, 79.66%, 81.07%, 83.47%, and 85.24%) of CA19-9, AFP, CA242, CEA, and CA72-4 in the tumor tissue of patients in observation group for the diagnosis of early gastric cancer are higherthan the sensitivity (78.66%, 79.25%, 76.18%, 82.11%, and 83.45%) and specificity (74.37%, 76.94%, 77.24%, 81.22%, and 81.59%) of that in control group with statistically significant differences (P < 0.05). Therefore, it is believed that the Fe3O4-PPy is of great significance for the detection of early gastric tumor markers in the tissues of patients with early gastric cancer, and has certain value for the auxiliary diagnosis of early gastric cancer and the observation of therapeutic effects. This study results provide a reference for the further researches of Fe3O4-PPy in the diagnosis of tumor markers in patients with early gastric cancer.

2.
Int J Biol Macromol ; 165(Pt B): 2378-2386, 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33132127

RESUMO

Ice recrystallization inhibitors have emerged as novel cryoprotectants to improve cell viability for cryopreservation. Nanocelluloses were identified as new materials for ice recrystallization inhibition (IRI); however, conventional nanocelluloses aggregate and lose IRI activity at high ionic strengths, which limit their application as cryoprotectants. In this study, we synthesized a novel group of nanocelluloses - electrosterically stabilized cellulose nanocrystals (ECNCs), which remained dispersed and IRI-active at high ionic strengths. ECNCs improved the post-thaw viability of HCT-116 colorectal cancer cells in slow/fast freezing-slow thawing protocols in the presence of 1-20% v/v dimethyl sulfoxide (DMSO), as well as in slow/fast freezing-fast thawing protocols at reduced DMSO concentrations. The effectiveness in cryoprotection did not match the IRI activity in ECNCs, polyethylene glycol (PEG), and polyvinyl alcohol (PVA); and in ECNCs with different surface charge densities. Overall, ECNCs demonstrated IRI and cryoprotection activities, but the mechanism of cryoprotection remains unknown.

3.
Phys Chem Chem Phys ; 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33147304

RESUMO

Effective detection of NO2 and NH3 gases at room temperature (RT) is critical for environmental monitoring and protection. Here, graphene-based gas sensors (Cu/Gr device) of single layer graphene decorated by 6, 8 and 10 nm thick Cu layers with graphene instead of conventional metal as interdigital electrodes are designed and fabricated. The RT performance for both NO2 and NH3 detection can be greatly enhanced by UV light illumination which is closely related to the thickness of Cu layers in which the device with 8 nm thickness (8 nm Cu/Gr device) exhibits the best performances. Analysis of XPS reveals that Cu is partly oxidized to Cu+ and Cu2+ for 6 nm with extra Cuδ+ (1 < δ < 2) for 8 and 10 nm. The contents and distributions of copper oxides and copper in Cu layers influence the catalytic effects and the heterojunction barrier and thus the performances. The RT responses of -30.9% and -8.1% for 5 and 0.3 ppm NO2, and of +29.1% and +5.9% for 105 and 10 ppm NH3 are achieved for the 8 nm Cu/Gr device, respectively. The limits of detection (LODs) for NO2 and NH3 are 12 ppb and 17 ppb, respectively. The sensing mechanisms are discussed in terms of density functional theory (DFT) calculations and energy band diagrams. The study demonstrates an effective solution of improving the device performance by modifying the device configuration and incorporating combined oxides naturally oxidized, which provides the novel design alternatives for high performance sensors.

4.
Sci Rep ; 10(1): 18892, 2020 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-33144674

RESUMO

Canopy architecture is critical in determining the light interception and distribution, and subsequently the photosynthetic efficiency and productivity. However, the physiological responses and molecular mechanisms by which pear canopy architectural traits impact on photosynthesis remain poorly understood. Here, physiological investigations coupled with comparative transcriptomic analyses were performed in pear leaves under distinct training systems. Compared with traditional freestanding system, flat-type trellis system (DP) showed higher net photosynthetic rate (PN) levels at the most time points throughout the entire monitored period, especially for the interior of the canopy in sunny side. Gene ontology analysis revealed that photosynthesis, carbohydrate derivative catabolic process and fatty acid metabolic process were over-represented in leaves of DP system with open-canopy characteristics. Weighted gene co-expression network analysis uncovered a significant network module positive correlated with PN value. The hub genes (PpFKF1 and PpPRR5) of the module were enriched in circadian rhythm pathway, suggesting a functional role for circadian clock genes in mediating photosynthetic performance under distinct training systems. These results draw a link between pear photosynthetic response and specific canopy architectural traits, and highlight light harvesting and circadian clock network as potential targets for the input signals from the fluctuating light availability under distinct training systems.

5.
Int J Biol Macromol ; 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33220380

RESUMO

The carboxymethylated (1 → 6)-α-dextran (CM-dex) was synthesized by introducing carboxymethyl groups at different degrees of substitution (DS). The resulting dex1-1, dex2-1, dex3-1, and dex4-1 products had degrees of substitution of 0.57, 0.78, 1.13, and 1.25, respectively. The dex3-1 showed the highest glass transition temperature (Tg) of 215.96 °C, whereas Tg of pure dextran was 149.83 °C. TGA results indicated that the residual loss was reduced along with the increase of DS in the high-temperature region (450-600 °C). Besides, the CM-dex had stronger scavenging capacity against OH radicals but lower scavenging capacity for DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals compared to that of pure dextran. The carboxymethylation of (1 → 6)-α-dextran will extend the applications for modified dextran.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33198468

RESUMO

Iron-based sulfides have been deemed as an appealing anode material for lithium-ion batteries (LIBs) and sodium-ion batteries (SIBs) for their high theoretical capacity and low cost. However, their practical application is limited by drastic volume expansion during cycling and low-intrinsic electronic conductivity. In this work, we report a FeS2/Fe7S8-rGO composite synthesized via a facile solvothermal method as an LIB/SIB anode. The FeS2/Fe7S8-rGO anode exhibits an excellent Li-storage capacity of 514 mAh g-1 at 2.0 A g-1 after 3000 cycles and a Na-storage capacity of 650 mAh g-1 at 0.2 A g-1 after 250 cycles, respectively. The rGO matrix is deemed responsible for providing good electron conduction and alleviating volume expansion during cycling. The electrokinetic analysis confirms a large portion of intercalational pseudocapacitance as a major contribution to the superior rate performance. In situ X-ray diffraction further reveals details of a combined intercalational and conversional Li-ion storage mechanisms in this Fe-sulfide-based anode. Finally, density functional theory calculations suggest that there exists a synergistic effect at the heterointerface between FeS2 and Fe7S8 to promote electrokinetics.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33190416

RESUMO

Increased sympathetic nervous system (SNS) activity leads to increased risk of cardiovascular morbidity and mortality. This study investigated whether there were sex differences in SNS activity among Chinese patients with hypertension. Ethnic Chinese non-diabetic hypertensive patients aged 20-50 years were enrolled in Taiwan. A total of 970 hypertensive patients (41.0 ± 7.2 years) completed the study, 664 men and 306 women. They received comprehensive evaluations including office blood pressure (BP) measurement, 24-h ambulatory BP monitoring, and 24-h urine sampling assayed for catecholamine excretion. Compared to women, men were younger, had higher body mass index (BMI), office systolic BP (SBP), office diastolic BP (DBP), 24-h ambulatory BP, and 24-h urine catecholamine excretion. In men, 24-h urine total catecholamine levels were correlated with 24-h SBP (r = 0.103, p = .008) and 24-h DBP (r = 0.083, p = .033). In women, however, there was no correlation between 24-h urine total catecholamine levels and 24-h ambulatory BP. Multivariate linear regression indicated that being male (ß = 1.65, 95% confidence interval [CI] 0.01-3.29, p = .048) and 24-h urine total catecholamine (ß = 5.03, 95% CI 0.62-9.44, p = .025) were both independently associated with 24-h SBP; being male was independently associated with 24-h DBP (ß = 3.55, 95% CI 2.26-4.85, p < .001). In conclusion, Chinese men with hypertension had higher SNS activity than women, and SNS activity was independently associated with 24-h ambulatory BP in men rather than in women. These findings suggest that different hypertensive treatment strategies should be considered according to patient sex.

8.
Technol Cancer Res Treat ; 19: 1533033820970663, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33153411

RESUMO

OBJECTIVE: Precision immunotherapy in non-small cell lung cancer (NSCLC) have been the focus of tumor immunity research. The aim of this study is to identify novel candidate biomarkers predicting the response to immunotherapy in NSCLC. METHODS: GSE126044 was obtained from Gene Expression Omnibus (GEO). According to the response to anti-PD-1 antibody, 2 groups were divided: response group and non-response group. Differentially expressed genes (DEGs) were screened using R. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. ROC curves and possible pathways of the seed genes were further analyzed. RESULTS: In total, 588 DEGs (487 upregulated DEGs and 101 downregulated) were identified. GO and KEGG analyses showed that upregulated DEGs were mainly enriched in immune response and cell adhesion pathways, while VEGF signaling pathway and metabolic pathways were mainly enriched in downregulated DEGs. In addition, CSF1 R and HCST showed more powerful predictive ability than PDL1. More importantly, these candidate genes were not only positively correlated with the expression of PDL1 and the infiltration of CD8+ T cells in the immune microenvironment, but also might improve the prognosis in lung squamous cell carcinoma. CONCLUSIONS: CSF1 R and HCST might be novel predictive markers for immunotherapy in NSCLC.

9.
Food Funct ; 11(11): 10058-10069, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33135718

RESUMO

Kaempferol has been confirmed to be effective in improving metabolic diseases such as diabetes and obesity. However, its effect and mechanism in nonalcoholic steatohepatitis (NASH) are unclear. We aim to confirm whether kaempferol could improve NASH and find the corresponding differential genes and metabolites. Transcriptomics combined with metabolomics was used to investigate the alterations in genes and metabolites expression after kaempferol treatment in mice with high-fat-diet-induced NASH. The results showed that kaempferol reduced the level of alanine transaminase (ALT), low-density lipoprotein cholesterol (LDL-C), and total cholesterol (TC) in serum and triglyceride (TG), lipid droplets, and inflammatory cell infiltration in liver. Further, 277 differentially expressed genes (DEGs) were identified through liver transcriptomics and the five most obvious DEGs were found to be CYP2b9, Cyp4a12b, Mup17, Mup7, and Mup16, which revealed that HFD induced fatty acid degradation, ribosome, and glyoxylic acid and dicarboxylic acid metabolism. Nine serum metabolites (methylcysteine, l-tryptophan, adrenic acid, d-2-hydroxyglutaric acid, tartaric acid, p-cresol sulfate, l-alanine, l-tryosine, and glutaconic acid) and 3 liver differential metabolites (gallic acid, γ-lindenic acid, and l-phenylalanine) were also identified, while the pathways were mainly involved in phenylalanine, tyrosine, and tryptophan biosynthesis; and phenylalanine metabolism. Integrating transcriptomics and metabolomics analyses indicated that kaempferol possesses the ability to improve NASH associated with energy metabolism, lipid metabolism, oxidative stress, and inflammation-related pathways. This study provides a powerful means of multiomics data integration and reveals the potent therapy and biomarkers for kaempferol.

10.
Food Funct ; 11(11): 9585-9598, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33151233

RESUMO

The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs). The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses. The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses. Besides, MLDs could alleviate dyslipidaemia, significantly suppress hepatic lipid accumulation and steatosis and effectively ameliorate lipid metabolism. The plasma LPS reduced significantly and the SCFAs increased in a dose-dependent manner. The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels. Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased. Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae). An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae was also observed. In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota. Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.

11.
Food Funct ; 11(11): 9662-9674, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33211056

RESUMO

The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences. We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice. The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice. Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment. In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased. Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood. More importantly, this may be closely related to alterations in the intestinal microbiota.

12.
Biosens Bioelectron ; : 112835, 2020 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-33246677

RESUMO

Exosomal miRNAs are potential tumor biomarkers for early diagnosis of non-small cell lung cancer (NSCLC). Herein, a surface plasmon resonance imaging (SPRi)-based biosensor was developed for simultaneous detection of multiplex NSCLC-associated exosomal miRNAs in a clinical sample using Au-on-Ag heterostructure and DNA tetrahedral framework (DTF). Exosomal miRNAs are captured by various DTF probes immobilized on the gold array chip. Subsequently, single-stranded DNA (ssDNA) functionalized silver nanocube (AgNC) hybridizes with the captured exosomal miRNAs and then the ssDNA-coated Au nanoparticles assembled on the surface of AgNC, forming Au-on-Ag heterostructures as essential labels to realize amplified SPR response. With the aid of DNA programmed Au-on-Ag heterostructure and DTF, the SPRi-based biosensor exhibits wide detection range from 2 fM to 20 nM, ultralow limit of detection of 1.68 fM, enhanced capture efficiency, and improved antifouling capability. Furthermore, the biosensor enables accurate discrimination of NSCLC patients based on detection results of exosomal miRNAs. Overall, this developed biosensor is a promising tool for multiplex exosomal miRNAs detection, providing a new possibility for early diagnosis of NSCLC.

13.
J Int Bioethique Ethique Sci ; Vol. 31(1): 63-83, 2020 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-33089678

RESUMO

For patients in critical or terminal situations, the approach to death is a situation that raises many ethical and legal issues of importance. Health professionals and families of the person at the end-of-life are obviously at the forefront of the concerns generated by these situations, all wishing to act in the best interest of the patient. Some States have regulated the various situations encountered on the ground in order to provide stakeholders with clear and appropriate procedures to ensure human dignity in practice by elaborating the rules of an end-of-life ethics aimed at supporting health professionals involved in carrying out certain acts while setting limits in respect of the persons concerned. What are the alternatives? Is euthanasia tolerable? What role for health professionals? And for the family? The content of the regulations differs according to countries, to cultural sensitivities and ethical and legal traditions, like the political will to regulate this topic. The regulatory experience of countries like France and China can be useful to other countries and serve as a basis for discussing the topic. Therefore, we analyze the current French framework and the situation in China in order to highlight main elements of ethical discussions, adopted positions and rules, eventual joint considerations, remaining issues and challenges in a comparative approach.


Assuntos
Eutanásia/ética , Pessoalidade , China , França , Humanos , Princípios Morais
14.
Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 2368-2371, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018482

RESUMO

It has been widely accepted that Parkinson's disease (PD) is triggered and shaped by propagation of misfolded α-synuclein. Converging neurophysiological evidence suggests that leucine-rich repeat kinase 2 (LRRK2) is involved in membrane transport of PD pathogenesis. This study proposed an agent-based computational model by integrating structural connections and gene expression to investigate whether LRRK2 would affect the PD pathology propagation in central nervous system. Gene expression profiles from the Allen Human Brain Atlas (AHBA) and multimodal brain MRI images from Parkinson's Progression Markers Initiative (PPMI) and Human Connectome Project (HCP) were employed for the model construction. The model results exhibit the involvement of LRRK2 gene expression remarkably elevated model fitting (r = 0.73) compared with the traditional susceptible-infected-removed (S-I-R) model (r=0.60). Specifically, our model revealed that LRRK2 is more likely to modulate pathology secretion out of neurons, rather than spreading into neurons. The findings support the theory of LRRK2 gene expression modulating cell-to-cell propagation of misfolded proteins. As a result, the proposed model would bring new insights of understanding PD mechanism in terms of misfolded α-synuclein propagation.


Assuntos
Doença de Parkinson , alfa-Sinucleína , Encéfalo/diagnóstico por imagem , Humanos , Serina-Treonina Proteína Quinase-2 com Repetições Ricas em Leucina/genética , Neurônios/metabolismo , Doença de Parkinson/genética , alfa-Sinucleína/genética
15.
Front Endocrinol (Lausanne) ; 11: 560508, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33117279

RESUMO

Background: Ultrasound-guided radiofrequency ablation (RFA) of thyroid nodules (TNs) is a minimally invasive procedure that has been widely used to induce volume reduction in symptomatic solid benign TNs. The goal of this study was to investigate a novel therapeutic approach for single-session ablation of large thyroid nodules (LTNs, vol > 20 ml). Methods: We performed a pilot cohort study of 21 patients with symptomatic solid benign LTNs (vol > 20 ml), who accepted ultrasound-guided RFA treatment between September 2018 and November 2019. RFA was performed using an 18-gauge internally cooled electrode with ultrasonographic guidance in a single session combined with intraoperative hydrodissection and immediate contrast-enhanced ultrasound (CEUS) to optimize safety and efficacy. Nodule volume was evaluated before ablation and at 1, 3, and 6 months after initial ablation, and all patients were asked to assess the cosmetic score (from 1 to 4) and symptom score (from 0 to 10) before ablation and at every follow-up after ablation. Results: At the 6 month follow-up, there was significant nodule volume reduction, from 27.49 ml ± 7.9 (standard deviation) to 3.82 ml ± 5.02 (p = 0.001). Cosmetic signs (p = 0.001) and pressure symptoms (p = 0.001) were significantly improved. All patients underwent RFA without any major complications, and very few patients developed a change in voice (2/21). However, the changes subsided within 1 month. Almost half of the patients received an additional RFA (11/21) treatment to achieve complete ablation on the intraoperative immediate CEUS evaluation. Conclusion: RFA is effective for treating LTNs (vol > 20 ml) and controlling clinical symptoms with a low complication rate. Patients were satisfied with cosmetic sign and pressure symptom improvement. The intraoperative hydrodissection and immediate CEUS represent a novel therapeutic approach for single-session ablation of LTNs.

16.
Artigo em Inglês | MEDLINE | ID: mdl-33121754

RESUMO

Poly (ethylene oxide) (PEO) polymer electrolyte, attracts great attention owing to its excellent flexibility, good processability and high safety compared with liquid electrolytes. However, its low ionic conductivity and weak ability to suppress the lithium dendrite severely restrict the further progress of PEO. Herein, we prepare a high ionic conductivity solid polymer electrolyte for all-solid-state lithium batteries by mixing PEO and magnetically aligned functionalized sepiolite (KFSEP) nanowires. The ionic conductivity of PEO/LiTFSI/10%⊥KFSEP solid polymer electrolyte is 2.0 × 10-5 S cm-1 at 20 °C (The ionic conductivity of PEO/LiTFSI solid polymer electrolyte is 4.0 × 10-7 S cm-1 at 20 °C). The experiments and simulation analysis indicate that the aligned nanowires provide a fast-moving channel for lithium ions. The capacity of Li/PEO/LiTFSI/10%⊥KFSEP/LFP cell is 130 mAh g-1 at 1.0 C under 60 °C after 450cycles. Furthermore, Li/PEO/LiTFSI/10%⊥KFSEP/LFP cell shows 150 mAh g-1 at 0.2 C under 25 °C. The Li/PEO/LiTFSI/10%⊥KFSEP/Li cell can work normally more than 600 h, indicating the high stability and lithium dendrite suppressing function of PEO/LiTFSI/10%⊥KFSEP. Overall, a high performance solid polymer electrolyte with higher safety is constructed by incorporating magnetically aligned sepiolite nanowires into PEO.

17.
Sensors (Basel) ; 20(19)2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33019643

RESUMO

Autonomous driving with artificial intelligence technology has been viewed as promising for autonomous vehicles hitting the road in the near future. In recent years, considerable progress has been made with Deep Reinforcement Learnings (DRLs) for realizing end-to-end autonomous driving. Still, driving safely and comfortably in real dynamic scenarios with DRL is nontrivial due to the reward functions being typically pre-defined with expertise. This paper proposes a human-in-the-loop DRL algorithm for learning personalized autonomous driving behavior in a progressive learning way. Specifically, a progressively optimized reward function (PORF) learning model is built and integrated into the Deep Deterministic Policy Gradient (DDPG) framework, which is called PORF-DDPG in this paper. PORF consists of two parts: the first part of the PORF is a pre-defined typical reward function on the system state, the second part is modeled as a Deep Neural Network (DNN) for representing driving adjusting intention by the human observer, which is the main contribution of this paper. The DNN-based reward model is progressively learned using the front-view images as the input and via active human supervision and intervention. The proposed approach is potentially useful for driving in dynamic constrained scenarios when dangerous collision events might occur frequently with classic DRLs. The experimental results show that the proposed autonomous driving behavior learning method exhibits online learning capability and environmental adaptability.

18.
Int J Biol Macromol ; 165(Pt B): 1978-1987, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33098902

RESUMO

A wheat germ polysaccharide (WGP-1) was isolated from wheat germ through hot-water (pH = 7.3) extraction and further fractionated by anion-exchange column using DEAE-52 cellulose column and purified by gel-permeation chromatography using Sephacryl S-500 column. WGP-1 was mainly consisted with rhamnose, arabinose, xylose, mannose, galactose and glucose, and linked with 1→, 1 â†’ 2, 1 â†’ 4, 1 â†’ 3 and 1 â†’ 6 glucosidic bands. The present study was designed to evaluate the antioxidant activities of WGP-1 in oleic acid (OA)-induced HepG2 cell model with its related mechanisms. Results showed that WGP-1 remarkably elevated the expression of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC), and decreased the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) in OA-treated cell. WGP-1 was found to promote the expression of PI3K, the phosphorylation of AKT and the translocation of Nrf2, further increased the expression of HO-1 in OA-induced cells. These results demonstrate again that WGP-1 can effectively improve OA-induced oxidative damage, probably by up-regulating of PI3K expression, Nrf2 translocation, and AKT phosphorylation.

19.
Infect Dis Poverty ; 9(1): 143, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33076968

RESUMO

BACKGROUND: Effective management of imported cases is an important part of epidemic prevention and control. Hainan Province, China reported 168 coronavirus disease 2019 (COVID-19), including 112 imported cases on February 19, 2020, but successfully contained the epidemic within 1 month. We described the epidemiological and clinical characteristics of COVID-19 in Hainan and compared these features between imported and local cases to provide information for other international epidemic areas. METHODS: We included 91 patients (56 imported and 35 local cases) from two designated hospitals for COVID-19 in Haikou, China, from January 20 to February 19, 2020. Data on the demographic, epidemiological, clinical and laboratory characteristics were extracted from medical records. Patients were followed until April 21, 2020, and the levels of antibodies at the follow-ups were also analysed by the Wilcoxon matched-pairs signed ranks test. RESULTS: Of the 91 patients, 78 (85.7%) patients were diagnosed within the first three weeks after the first case was identified (Day 1: Jan 22, 2020), while the number of local cases started to increase during the third week. No new cases occurred after Day 29. Fever and cough were two main clinical manifestations. In total, 15 (16.5%) patients were severe, 14 (15.4%) had complicated infections, nine (9.9%) were admitted to the intensive care unit, and three died. The median duration of viral shedding in feces was longer than that in nasopharyngeal swabs (19 days vs 16 days, P = 0.007). Compared with local cases, imported cases were older and had a higher incidence of fever and concurrent infections. There was no difference in outcomes between the two groups. IgG was positive in 92.8% patients (77/83) in the follow-up at week 2 after discharge, while 88.4% patients (38/43) had a reduction in IgG levels in the follow-up at week 4 after discharge, and the median level was lower than that in the follow-up at week 2 (10.95 S/Cut Off (S/CO) vs 15.02 S/CO, P <  0.001). CONCLUSION: Imported cases were more severe than local cases but had similar prognoses. The level of IgG antibodies declined from week 6 to week 8 after onset. The short epidemic period in Hainan suggests that the epidemic could be quickly brought under control if proper timely measures were taken.


Assuntos
Doenças Transmissíveis Importadas/epidemiologia , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/epidemiologia , Pneumonia Viral/diagnóstico , Pneumonia Viral/epidemiologia , Adulto , Idoso , Betacoronavirus/isolamento & purificação , China/epidemiologia , Doenças Transmissíveis Importadas/diagnóstico , Doenças Transmissíveis Importadas/terapia , Doenças Transmissíveis Importadas/virologia , Infecções por Coronavirus/virologia , Fezes/virologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/virologia , Estudos Retrospectivos , Tórax/diagnóstico por imagem , Resultado do Tratamento , Eliminação de Partículas Virais
20.
J BUON ; 25(4): 2045-2050, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33099951

RESUMO

PURPOSE: Prostate cancer causes significant mortality and microRNAs (miRs) have been shown to regulate the growth and metastasis of different cancers. In this context, the present study was designed to investigate the potential of miR-151 in the treatment of prostate cancer. METHODS: The normal and the prostate cancer cell lines (LNCaP, PC-3 and Du-145) were used in this study. The expression of miR-151 was determined by qRT-PCR. The DAPI and annexin V/propidium iodide (PI) staining were used for the detection of apoptosis. Transwell assay was used for the estimation of cell migration and invasion. Western blot analysis was used for the determination of the protein expression. RESULTS: miR-151 was downregulated in prostate cancer cells and showed inhibitory effect on cell growth which was manifested as decline in cell survival and loss of viability of cancer cells. Additionally, the chemosensitivity of prostate cancer cells to 5-FU was enhanced under miR-151 overexpression. Furthermore, miR-151 also inhibited the migration and invasion of cancer cells. The results of western blot analysis showed that miR-151 overexpression blocks the Pi3K/AKT signalling pathway in prostate cancer cells. CONCLUSION: Taken together, miR-151 has growth inhibitory effect against prostate cancer and negatively regulates the cell migration and invasion along with enhancement of chemosensitivity of cancer cells.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA