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1.
Food Chem ; 304: 125377, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476547

RESUMO

We devise a novel colorimetric aptasensor for multiplex antibiotics based on an ss-DNA fragment coordinately controlling gold nanoparticles (AuNPs) aggregation. The multifunctional aptamer (Apt) was elaborately designed to be adsorbed on AuNPs surfaces acting as a binding element for antibiotics and a molecular switch. Chloramphenicol (CAP) and tetracycline (TET) were selected as the model antibiotics. When one kind of antibiotics was added, the specifically recognized fragment of Apt can bind to it and dissociated, and the non-specific one coordinately controls AuNPs aggregation under high-salt conditions. Hence, different color changes of AuNPs solution can be used as the signal readout. The aptasensor exhibited remarkable selectivity and sensitivity for separate detection of TET and CAP, and the detection limits are estimated to be 32.9 and 7.0 nM, respectively. The analysis with the absorption spectroscopy and the smartphone are applied to detect antibiotics in real samples with consistent results and desirable recoveries.

2.
Bioresour Technol ; 295: 122278, 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31669867

RESUMO

This study explored the influences of peroxydisulfate (PDS) and peroxymonosulfate (PMS) activated with different catalysts on the anaerobic fermentation of waste activated sludge (WAS). All the treatments were effective in promoting short-chain fatty acids (SCFAs) production, particularly acetic acid, in the order of PMS/MnO2 > PMS/Zn > PDS/Zn > PMS/Fe > PDS/Fe > PDS/MnO2. Mechanistic investigations demonstrated that WAS disintegration was intensely induced by the free radicals (i.e., SO4- and OH) generated in PDS and PMS treating reactors. It significantly promoted the solubilization and hydrolysis processes and thereby provided sufficient bioavailable substrates for further acidogenic metabolisms. Additionally, it enlarged the abundance of functional bacteria responsible for SCFAs production. The simultaneous promotion of bioavailable substrates and fermentative microorganisms markedly contributed to the SCFAs enhancement. Moreover, the dewaterability and stabilization of fermented sludge were both improved with the PDS and PMS treatments, which were beneficial to the final disposal of WAS.

3.
Water Res ; 169: 115202, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31669899

RESUMO

The degradation of tetrabromobisphenol A (TBBPA), one of the most widely used brominated flame retardant, was evaluated during peroxymonosulfate (PMS) oxidation. TBBPA degradation was pH-dependent, with peak degradation rate constants occurring at pH 8.0-9.0, which was distinct from some other phenolic compounds. Singlet oxygen and radicals were found to play negligible roles in TBBPA degradation. TBBPA oxidation by PMS mainly proceeded via a direct oxidation pathway and the in-situ formed HOBr was found to greatly accelerate its degradation rates. The values of species-specific second-order rate constants for the reactions of PMS with the TBBPA kHSO5-+TBBPA, kHSO5-+TBBPA- and kHSO5-+TBBPA2- were determined to be (1.11 ±â€¯0.84) × 10-2, (8.05 ±â€¯2.31) × 10-2, and (1.34 ±â€¯0.25) × 10-1 M-1 s-1, respectively, while the reaction rate constants for HOBr/OBr- with TBBPA kHOBr+TBBPA, kHOBr+TBBPA-, kHOBr+TBBPA2-and kOBr-+TBBPA2- were determined to be (9.38 ±â€¯2.10) × 103, (1.59 ±â€¯0.56) × 105, (8.22 ±â€¯0.41) × 106, and (1.81 ±â€¯0.12) × 106 M-1 s-1, respectively. The bromine mass balance analysis showed that bromide ion and HOBr/OBr- occupied 19.5% of total Br and brominated organic compounds accounted for the remaining percentages at pH 7.0. No formation of bromate was observed. Based on the identified products, a reaction pathway was proposed, which included oxidation, ß-scission, hydroxylation, and dimerization reaction pathways. The results indicate that unactivated PMS is useful for the remediation of TBBPA contaminated water.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31640233

RESUMO

Recently, the growing release of CeO2 nanoparticles (CeO2 NPs) into sewage systems has attracted great concern. Several studies have extensively explored CeO2 NPs' potential adverse impacts on wastewater treatment plants; however, the impaired activated sludge recovery potentials have seldom been addressed to date. To explore the physicochemical and biological effects on the activated sludge performance and activity recovery of damaged sludge by exposure to CeO2 NPs in sequencing batch reactors (SBRs), four reactors and multiple indicators including water quality, key enzymes, microbial metabolites, the microbial community structure and toxicity were used. Results showed that 10-week exposure to higher CeO2 NP concentration (1, 10 mg/L) resulted in a sharp decrease in nitrogen and phosphorus removal efficiencies, which were consistent with the tendencies of key enzymes. Meanwhile, CeO2 NPs at concentrations of 0.1, 1, and 10 mg/L decreased the secretion of tightly bound extracellular polymeric substances to 0.13%, 3.14%, and 28.60%, respectively, compared to the control. In addition, two-week recovery period assays revealed that the functional bacteria Proteobacteria, Nitrospirae and Planctomycetes recovered slightly at the phyla level, as analyzed through high-throughput sequencing, which was consistent with the small amount of improvement of the effluent performance of the system. This reflected the small possibility of the activity recovery of damaged sludge.

5.
Anal Bioanal Chem ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31641824

RESUMO

A label-free, rapid response colorimetric aptasensor for sensitive detection of chloramphenicol (CAP) was proposed, which was based on the strategy of ssDNA-modified gold nanoparticle (AuNP) aggregation assisted by lanthanum (La3+) ions. The AuNPs generated a color change that could be monitored in the red, green, and blue and analyzed by the smartphone imaging app. La3+, as a trigger agent, strongly combined with the phosphate groups of the surface of ssDNA-AuNPs probe, which helps create AuNP aggregation and the color change of AuNPs from red to blue. On the contrary, when mixing with CAP, the aptamer (Apt) bound to CAP to form a rigid structure of the Apt-CAP complex, and La3+ attached to the phosphate groups of the complex, which prevented the aptamer from binding to the surface of the AuNPs. As a result, the color of the AuNPs changed to violet-red. Finally, UV-vis absorption spectroscopy and the smartphone imaging app were employed to determine CAP with a lower detection limit of 7.65 nM and 5.88 nM, respectively. The proposed strategy featuring high selectivity and strong anti-interference ability for detection of CAP in practical samples was achieved. It is worth mentioning that the simple and portable colorimetric aptasensor will be used for facilitating on-site detection of food samples.

6.
Nat Commun ; 10(1): 4464, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31578336

RESUMO

The use of an endogenous stimulus instead of external trigger has an advantage for targeted and controlled release in drug delivery. Here, we report on cascade nanoreactors for bacterial toxin-triggered antibiotic release by wrapping calcium peroxide (CaO2) and antibiotic in a eutectic mixture of two fatty acids and a liposome coating. When encountering pathogenic bacteria in vivo these nanoreactors capture the toxins, without compromising their structural integrity, and the toxins form pores. Water enters the nanoreactors through the pores to react with CaO2 and produce hydrogen peroxide which decomposes to oxygen and drives antibiotic release. The bound toxins reduce the toxicity and also stimulate the body's immune response. This works to improve the therapeutic effect in bacterially infected mice. This strategy provides a Domino Effect approach for treating infections caused by bacteria that secrete pore-forming toxins.

7.
Neuromolecular Med ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31595404

RESUMO

Optogenetic stimulation of neural stem cells (NSCs) enables their activity-dependent photo-modulation. This provides a spatio-temporal tool for studying activity-dependent neurogenesis and for regulating the differentiation of the transplanted NSCs. Currently, this is mainly driven by viral transfection of channelrhodopsin-2 (ChR2) gene, which requires high irradiance and complex in vivo/vitro stimulation systems. Additionally, despite the extensive application of optogenetics in neuroscience, the transcriptome-level changes induced by optogenetic stimulation of NSCs have not been elucidated yet. Here, we made transformed NSCs (SFO-NSCs) stably expressing one of the step-function opsin (SFO)-variants of chimeric channelrhodopsins, ChRFR(C167A), which is more sensitive to blue light than native ChR2, via a non-viral transfection system using piggyBac transposon. We set up a simple low-irradiance optical stimulation (OS)-incubation system that induced c-fos mRNA expression, which is activity-dependent, in differentiating SFO-NSCs. More neuron-like SFO-NCSs, which had more elongated axons, were differentiated with daily OS than control cells without OS. This was accompanied by positive/negative changes in the transcriptome involved in axonal remodeling, synaptic plasticity, and microenvironment modulation with the up-regulation of several genes involved in the Ca2+-related functions. Our approach could be applied for stem cell transplantation studies in tissue with two strengths: lower carcinogenicity and less irradiance needed for tissue penetration.

8.
J Formos Med Assoc ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31607629

RESUMO

BACKGROUND/PURPOSE: Our previous study found that 180 of 1064 atrophic glossitis (AG) patients have iron deficiency. This study assessed whether all AG patients with iron deficiency (so-called ID/AG patients) had iron deficiency anemia (IDA) and evaluated whether the ID/AG patients had significantly higher frequencies of anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity than healthy control subjects. METHODS: The blood hemoglobin (Hb) and serum iron, vitamin B12, folic acid, homocysteine, and GPCA levels in 180 ID/AG patients and 532 healthy control subjects were measured and compared. RESULTS: We found that 180 ID/AG patients had significantly lower mean corpuscular volume (MCV) and lower mean blood Hb and serum iron levels as well as significantly higher mean serum homocysteine level than healthy control subjects (all P-values < 0.001). Moreover, 180 ID/AG patients had significantly higher frequencies of blood Hb (46.1%), serum iron (100.0%), vitamin B12 (8.3%), and folic acid (4.4%) deficiencies, hyperhomocysteinemia (16.1%), and serum GPCA positivity (31.1%) than 532 healthy control subjects (all P-values < 0.001). In addition, of 83 anemic ID/AG patients, 9 (10.8%) had pernicious anemia, 40 (48.2%) had normocytic anemia, 30 (36.2%) had IDA, and 4 (4.8%) had thalassemia trait-induced anemia. CONCLUSION: We conclude that ID/AG patients had significantly higher frequencies of blood Hb, serum iron, vitamin B12, and folic acid deficiencies, hyperhomocysteinemia, and serum GPCA positivity than 532 healthy control subjects. Normocytic anemia is the most common type of anemia in ID/AG patients, followed by IDA, pernicious anemia, and thalassemia trait-induced anemia.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31634836

RESUMO

Person re-identification (Re-ID) aims at matching person images captured in non-overlapping camera views. To represent person appearance, low-level visual features are sensitive to environmental changes, while high-level semantic attributes, such as "short-hair" or "long-hair", are relatively stable. Hence, researches have started to design semantic attributes to reduce the visual ambiguity. However, to train a prediction model for semantic attributes, it requires plenty of annotations, which are hard to obtain in practical large-scale applications. To alleviate the reliance on annotation efforts, we propose to incrementally generate Deep Hidden Attribute (DHA) based on baseline deep network for newly uncovered annotations. In particular, we propose an auto-encoder model that can be plugged into any deep network to mine latent information in an unsupervised manner. To optimize the effectiveness of DHA, we reform the auto-encoder model with additional orthogonal generation module, along with identity-preserving and sparsity constraints. 1) Orthogonally generating: In order to make DHAs different from each other, Singular Vector Decomposition (SVD) is introduced to generate DHAs orthogonally. 2) Identity-preserving constraint: The generated DHAs should be distinct for telling different persons, so we associate DHAs with person identities. 3) Sparsity constraint: To enhance the discriminability of DHAs, we also introduce the sparsity constraint to restrict the number of effective DHAs for each person. Experiments conducted on public datasets have validated the effectiveness of the proposed network. On two large-scale datasets, i.e., Market-1501 and DukeMTMC-reID, the proposed method outperforms the state-of-the-art methods.

10.
Intern Med J ; 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31589357

RESUMO

BACKGROUND: Therapeutic drug monitoring (TDM) of infliximab (IFX) levels in inflammatory bowel disease (IBD) patients can help to guide dose adjustments or changes to therapy for selected patients in remission or with secondary loss of response. AIMS: To determine how IFX TDM is utilised in a real-life clinical setting and to quantify the potential for TDM to reduce the unnecessary use of IFX. METHODS: Data from all public IBD IFX level testing performed across Australia was prospectively collected from June 2016 to July 2017 to assess physician-reported for testing indications (induction, in remission or loss of response) and associated results. The hypothetical influence of IFX TDM was based on an optimal therapeutic range of 6-10 mg/L for mucosal healing. RESULTS: Secondary loss of response (reactive TDM) was the most common indication for TDM. These patients have consistently lower median IFX levels: 3.02 mg/L (IQR 1.14 - 6.67 mg/L) vs. 5.22 mg/L (IQR 2.70 - 8.12 mg/L), p = 0.0001 compared with patients in remission (proactive TDM). TDM helped to identify unnecessary use of IFX in 30.6% of the TDM tests performed in luminal Crohn's disease and ulcerative colitis patients, with an associated drug cost saving of $531.38 per IFX TDM test episode. Unnecessary IFX use was identified in 38.9% (96/247) of reactive IFX TDM tests performed and in 19.3% (35/181) of proactive testing. CONCLUSION: Use of both reactive and proactive IFX TDM is cost-effective for IBD management as it informs the clinician where unnecessary use of IFX can be stopped. This article is protected by copyright. All rights reserved.

11.
J Transl Med ; 17(1): 341, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601236

RESUMO

BACKGROUND: The role of body fat distribution in uric acid metabolism is still ambiguity. We aimed to investigate the independent contribution of visceral adipose measured by visceral adiposity index and lipid accumulation product and liver fat assessed by fatty liver index to the risk of hyperuricemia. METHODS: We conducted a cross-sectional study involving 1284 participants aged ≥ 40 years old recruited from communities in Zhonglou district, Changzhou. Each participant completed a standard questionnaire, and provided blood samples for biochemical measurements. Visceral adiposity index, fatty liver index and lipid accumulation product were calculated by simple anthropometric and functional parameters. Hyperuricemia was defined as serum uric acid ≥ 420 µmol/l for males and ≥ 360 µmol/l for females. RESULTS: The prevalence of hyperuricemia was 15.9% and gradually increased across tertiles of adiposity-based indices. The visceral adipose-based measurements (visceral adiposity index, fatty liver index, lipid accumulation product) had better power to discriminate hyperuricemia than body mass index (BMI), waist circumference and neck circumference, and visceral adiposity index exhibited the highest power, with the area under the receiver operating characteristics curve (AUROC) of 0.662 (0.636-0.688). Multivariate logistic regression found 1.49-fold, 2.21-fold and 2.12-fold increased risk of hyperuricemia with 1-unit increment of visceral adiposity index, fatty liver index, and lipid accumulation product, respectively. Compared to tertile 1, the odds ratios of hyperuricemia for the second tertile and the third tertile of visceral adiposity index were 1.57 (1.00-2.50) and 3.11 (1.96-4.94), those of fatty liver index were 1.64 (1.05-2.68) and 3.58 (1.94-6.01), and those of lipid accumulation product were 1.93 (1.19-3.15) and 3.53 (2.05-6.09), respectively. However, no significant associations of BMI, waist circumference and neck circumference with hyperuricemia were observed. CONCLUSIONS: Visceral adipose accumulation increased the risk of hyperuricemia, independently of BMI, waist circumference and neck circumference, among middle-aged and elderly Chinese adults.

12.
Hum Gene Ther Methods ; 30(5): 172-183, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31566024

RESUMO

Recombinant adeno-associated viruses (rAAVs) are excellent vectors for gene delivery. However, current Sf9/Cap-Rep packaging cell line-dependent OneBac systems still lack versatility and flexibility for large-scale production of rAAVs. In this study, we developed an improved OneBac system that includes a novel dual-function baculovirus expression vector (BEV) termed BEV/Cap-(ITR-GOI) that carries both the AAV Cap gene and rAAV genome inverted terminal repeat (ITR) sequences flanking the gene of interest (GOI), a versatile Sf9-GFP/Rep packaging cell line that harbors silent copies of the AAV2 Rep gene that can be expressed after BEV infection, and constitutively expressed green fluorescent protein (GFP) reporter genes to facilitate cell line screening. The BEV/Cap-(ITR-GOI) construct allows flexibility to switch among different Cap gene serotypes using simple BEV reconstruction, and is stable for at least five serial passages. Furthermore, the Sf9-GFP/Rep stable cell line is versatile for production of different rAAV serotypes. The yield levels for rAAV2, rAAV8, and rAAV9 exceeded 105 vector genomes (VG) per cell, which is similar to other currently available large-scale rAAV production systems. The new Bac system-derived rAAVs have biophysical properties similar to HEK293 cell-derived rAAVs, as well as high quality and activity. In summary, the novel Sf9-GFP/Rep packaging cell line-dependent OneBac system can facilitate large-scale rAAV production and rAAV-based gene therapy.

13.
J Formos Med Assoc ; 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31630933

RESUMO

BACKGROUND/PURPOSE: Our previous study found that 56 of 1064 atrophic glossitis (AG) patients have vitamin B12 deficiency. This study assessed whether the AG patients with vitamin B12 deficiency (B12D/AG patients) had significantly higher frequencies of anemia, hematinic deficiencies, hyperhomocysteinemia, and serum gastric parietal cell antibody (GPCA) positivity than healthy control subjects. METHODS: The blood hemoglobin (Hb) and serum iron, vitamin B12, folic acid, homocysteine, and GPCA levels in 56 B12D/AG patients and 532 healthy control subjects were measured and compared. RESULTS: We found that 56 B12D/AG patients had significantly lower mean blood Hb and serum iron levels as well as significantly higher mean corpuscular volume (MCV) and mean serum homocysteine level than healthy control subjects (all P-values < 0.05). Moreover, 56 B12D/AG patients had significantly higher frequencies of macrocytosis (53.6%), blood Hb (64.3%), iron (26.8%), and folic acid (3.6%) deficiencies, hyperhomocysteinemia (89.3%), and serum GPCA positivity (55.4%) than 532 healthy control subjects (all P-values < 0.005). In addition, of 36 anemic B12D/AG patients, 22 (61.1%) had pernicious anemia (PA), 6 (16.7%) had macrocytic anemia other than PA, 4 (11.1%) had normocytic anemia, 3 (8.3%) had iron deficiency anemia (IDA), and one (2.8%) had microcytic anemia other than IDA and thalassemia trait-induced anemia. CONCLUSIONS: We conclude that B12D/AG patients have significantly higher frequencies of macrocytosis, blood Hb, iron, and folic acid deficiencies, hyperhomocysteinemia, and serum GPCA positivity than healthy control subjects. PA is the most common type of anemia in our B12D/AG patients.

14.
Arch Gynecol Obstet ; 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31631247

RESUMO

PURPOSE: Recurrent implantation failure (RIF) is a common cause of disappointment and a big challenge after assisted reproduction technology treatments. The objective of this study was to evaluate the existing literature to explore whether peripheral blood mononuclear cells' (PBMCs) instillation could improve pregnancy outcomes among patients with RIF. METHODS: We conducted a comprehensive search including PubMed, EMBASE, Cochrane library and various databases in China. Three randomized controlled trials (RCTs) and three non-randomized controlled trials (non-RCTs) were included. We included subgroup and sensitivity analyses using Stata 12.0. RESULTS: The results of the three RCTs showed that PBMC improved outcomes in all patients compared with placebo or no-treatment [clinical pregnancy rate (CPR): odds ratio (OR) 2.45, 95% confidence interval (CI) 1.53-3.91; implantation rate (IR): OR 2.46, 95% CI 1.48-4.09; live birth rate (LBR): OR 2.43, 95% CI 1.32-4.49]. However, the results of the three non-RCTs indicated that there were no statistically significant differences in the outcomes and that the heterogeneity was higher (I2 > 0%). Subgroup analysis further suggested that PBMCs treatment significantly increased the CPR, IR and LBR in the three or more implantation failure subgroups (CPR: OR 2.83, 95% CI 1.29-6.22; IR: OR 3.74, 95% CI 1.71-8.19; LBR: OR 3.03, 95% CI 1.15-7.98). CONCLUSIONS: Among patients with three or more implantation failures, this treatment improved IR, LBR, and CPR compared to that in controls, due to the limited data available, PBMCs' intrauterine instillation should only be used in the context of clinical trials.

15.
Medicine (Baltimore) ; 98(37): e16974, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517816

RESUMO

BACKGROUND: Growing evidence showed that high pretreatment plasma fibrinogen could be used as a potential prognostic marker in colorectal cancer (CRC). However, the conclusions were controversial. Therefore, this meta-analysis was conducted to evaluate the prognostic value of pretreatment plasma fibrinogen in patients with CRC. METHODS: Relevant studies were searched in the databases including PubMed, EMBASE, Web of Science, Cochrane library, and China National Knowledge Infrastructure up until December 10th, 2018. Pooled hazard ratios (HRs) with their 95% confidence intervals (CIs) were used to estimate the effects. RESULTS: A total of 17 articles with 6863 patients were included in this meta-analysis. The results revealed that elevated pretreatment plasma fibrinogen was significantly associated with both poor overall survival (univariate analysis: HR = 1.69, 95% CI 1.47-1.95, P = .000; multivariate analysis: HR = 1.50, 95% CI 1.28-1.77, P = .000) and poor disease-free survival (univariate analysis: HR = 1.90, 95% CI 1.49-2.41, P = .000; multivariate analysis: HR = 2.08, 95% CI 1.52-2.86, P = .000) in patients with CRC. CONCLUSIONS: High pretreatment plasma fibrinogen level is significantly associated with worse survival outcomes in CRC patients. Plasma fibrinogen may be used as an effective prognostic marker and potential therapeutic target. Further studies are required to support these results.


Assuntos
Neoplasias Colorretais/sangue , Fibrinogênio/análise , Biomarcadores/sangue , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/terapia , Humanos , Prognóstico
16.
Sensors (Basel) ; 19(18)2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31546976

RESUMO

In this article, a gait recognition algorithm is presented based on the information obtained from inertial sensors embedded in a smartphone, in particular, the accelerometers and gyroscopes typically embedded on them. The algorithm processes the signal by extracting gait cycles, which are then fed into a Recurrent Neural Network (RNN) to generate feature vectors. To optimize the accuracy of this algorithm, we apply a random grid hyperparameter selection process followed by a hand-tuning method to reach the final hyperparameter configuration. The different configurations are tested on a public database with 744 users and compared with other algorithms that were previously tested on the same database. After reaching the best-performing configuration for our algorithm, we obtain an equal error rate (EER) of 11.48% when training with only 20% of the users. Even better, when using 70% of the users for training, that value drops to 7.55%. The system manages to improve on state-of-the-art methods, but we believe the algorithm could reach a significantly better performance if it was trained with more visits per user. With a large enough database with several visits per user, the algorithm could improve substantially.

17.
Biomaterials ; 223: 119472, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31499254

RESUMO

Inflammation during photothermal therapy (PTT) of tumor usually results in adverse consequences. Here, a biomembrane camouflaged nanomedicine (mPDAB) containing polydopamine and ammonia borane was designed to enhance PTT efficacy and mitigate inflammation. Polydopamine, a biocompatible photothermal agent, can effectively convert light into heat for PTT. Ammonia borane was linked to the surface of polydopamine through the interaction of hydrogen bonding, which could destroy redox homoeostasis in tumor cells and reduce inflammation by H2 release in tumor microenvironment. Owing to the same origin of outer biomembranes, mPDAB showed excellent tumor accumulation and low systemic toxicity in a breast tumor model. Excellent PTT efficacy and inflammation reduction made the mPDAB completely eliminate the primary tumors, while also restraining the outgrowth of distant dormant tumors. The biomimetic nanomedicine shows potentials as a universal inflammation-self-alleviated platform to ameliorate inflammation-related disease treatment, including but not limited to PTT for tumor.

18.
Bioresour Technol ; 293: 122088, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31499331

RESUMO

This study presented an innovative method for phosphorus (P) recovery as vivianite from waste activated sludge (WAS) via optimizing iron dosing and pH value during anaerobic fermentation (AF). The optimal conditions for vivianite formation were in the pH range of 6.0-9.0 with initial PO43- >5 mg/L and Fe/P molar ratio of 1.5. Notably, FeCl3 showed advantages over ZVI for the simultaneous release of Fe2+ and PO43- during WAS fermentation, especially in acidic conditions. The FeCl3 dosing at pH 3.0 could contribute to 78.81% Fe2+ release and 85.69% of total PO43- release from WAS. They were ultimately recovered in the form of high-purity vivianite (93.67%). Clostridiaceae (40.25%) was the predominant bacteria in FeCl3-pH3 reactors, which played key roles in inducing dissimilatory iron reduction for Fe2+ formation. Therefore, P recovery as vivianite from WAS fermentation might be a promising and highly valuable approach to relieve the P crisis.


Assuntos
Fósforo , Esgotos , Anaerobiose , Fermentação , Compostos Ferrosos , Concentração de Íons de Hidrogênio , Ferro , Fosfatos , Eliminação de Resíduos Líquidos
19.
Dig Dis Sci ; 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31493039

RESUMO

BACKGROUND: Retrospective studies observe an increased risk of keratinocyte carcinomas (KCs) in patients with inflammatory bowel disease (IBD) on thiopurine (TP) medication. The role of traditional risk factors such as skin type and sun protection behavior has not been studied in this population. This study aimed to examine traditional KC risk factors and thiopurine use on skin cancer development in an IBD cohort. METHODS: Consecutive IBD patients were recruited from four specialist centers in Australia and New Zealand, each with varying UV exposure indices. Data pertaining to race, skin color, freckling and sun protection behavior, dose of TP therapy, and skin cancer development were elicited through a self-reported questionnaire. RESULTS: A total of 691 IBD patients were included with 62 reporting KC development. Thiopurine usage was similar among patients who developed skin cancer compared with those who did not (92% vs. 89%, p = 0.3). There was no statistically significant association between KC development and TP dose or 6-thioguanine nucleotide levels. In multivariate modeling, four factors were independently and significantly associated with KC: age over 61 years old versus less than 30 years old (OR 6.76; 95% CI 2.38-19.18), residing in Brisbane versus Christchurch (OR 3.3; 95% CI 1.6-6.8), never staying in the shade versus staying in the shade ≥ 50% of the time (OR 3.8; 95% CI 1.4-10.5), and having a skin type that never tanned versus other skin types (OR 6.9; 95% CI 2.9-16.0). CONCLUSION: Skin type, age, and sun protection behavior are more important risk factors for KC development than thiopurine medication use in this IBD population.

20.
Mol Carcinog ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31513316

RESUMO

Ovarian cancer is the most lethal gynecologic malignancy due to the lack of symptoms until advanced stages, and new diagnosis and treatment strategy is in urgent need. In this study, we found higher expression of miR-19a-3p in ovarian cancer tissues compared with that in the adjacent normal tissues. By chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) analysis, we showed that nuclear factor-kappaB (NF-κB) binds to the promoter of miR-19a-3p, leading to reduced expression in ovarian cancer cells. Further study indicated that miR-19a-3p inhibits the expression of insulin-like growth factor binding protein-3 (IGFBP-3), resulting in enhanced growth and migration of ovarian cancer cells in vitro and tumor growth in vivo. These results showed that miR-19a-3p enhances the oncogenesis of ovarian cancer through inhibition of IGFBP-3 expression, and which can be inhibited by NF-κB, suggesting an NF-κB/miR-19a-3p/IGFBP-3 pathway in the oncogenesis of ovarian cancer, which expands our understanding of ovarian cancer and they may contribute to the development of new diagnosis and treatment of ovarian cancer.

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