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1.
Immunol Invest ; : 1-20, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31985304

RESUMO

Our previous study reveals that gamma delta (γδ) T cells were activated and dendritic cells (DCs) underwent maturation during the inflammation phase in experimental autoimmune uveitis (EAU) mice, and the interaction between DCs and γδ T cells may significantly exacerbate the development of EAU. However, the interactions between DCs and γδ T cells that can affect DCs maturation to influence EAU development must be further addressed. In this study we showed that mature DC numbers in TCR-δ-/- (KO) EAU mice were lower than those in wild-type (WT) C57BL/6 (B6) mice. The γδ T cells harvested from WT EAU mice secreted more interferon-γ (IFN-γ), however, after blocking IFN-γ, the maturation of DCs was significantly downregulated. By contrast, the percentage of IFN-γ- and IL-17-producing CD4+ T cells in KO EAU mice decreased to a greater extent than that in WT EAU mice during the inflammatory phase. Additionally, the levels of IFN-γ/IL-17 in serum were in agreement with those of CD4+ T cells. Furthermore, after activated γδ T cells injection, the inflammatory symptoms of EAU mice were more aggravated. In vitro co-cultures of both cell types showed that activated γδ T cells may induce DCs to generate higher levels of intracellular cell adhesion molecule-1 (ICAM-1/CD54), CD80, CD83, and CD86. Moreover, co-culture of the two cells may induce the activation of CD4+ T cells. Taken together, our results demonstrated that activated γδ T cells may promote DCs maturation and further enhance the generation of Th1/Th17 cells in EAU mice, resulting in exacerbated EAU.

2.
J Hazard Mater ; 386: 121962, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31911380

RESUMO

Here, we report the photocatalytic oxidation (PCO) of o-xylene on carbon quantum dots (CQDs) modified TiO2 nanoparticles. The results demonstrated that with 1 wt% CQDs loading, 87 % of o-xylene (50 ppm) can be photodegraded, which is 55.3 % higher than pure TiO2 (56 %) under UV/visible light. This improved photocatalytic activity is associated with the important role of CQDs on TiO2 surface, which increased the o-xylene adsorption and facilitated the photogenerated hole-electron separation process. Also, the 1 wt%CQDs/TiO2 nanocomposite showed photocatalytic activity in the visible region (λ > 400 nm) compared to pure TiO2 (inactive). The DFT study revealed that o-xylene strongly adsorb on TiO2 (001) surface than (101) through π electrons of the aromatic ring. The in situ DRIFTS study showed that free OH groups on the photocatalyst surface could act as effective Lewis sides for the o-xylene adsorption. The interaction of π electrons of the aromatic ring and isolated OH groups was also observed. The FTIR peaks for CO2 increased in the case of CQDs/TiO2 nanocomposite contrasted to pure TiO2, which suggested that the presence of CQDs improved the mineralization potency of TiO2. These findings should affect the quest for a better photocatalyst to photodegrade VOCs.

3.
J Sep Sci ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960568

RESUMO

A newly developed portable capillary liquid chromatograph was investigated for the separation of various pharmaceutical and illicit drug compounds. The system consists of two high-pressure syringe pumps capable of delivering capillary-scale flow rates at pressures up to 10 000 psi. Capillary liquid chromatography columns packed with sub-2 µm particles are housed in cartridges that can be inserted into the system and easily connected through high-pressure fluidic contact points by simply applying a specific, predetermined torque rather than using standard fittings and less precise sealing protocols. Several over-the-counter analgesic drug separations are demonstrated, along with a simple online measurement of tablet dissolution. Twenty illicit drug compounds were also separated across six targeted drug panels. The results described in this study demonstrate the capability of this compact liquid chromatography instrument to address several important drug-related applications while simplifying system operation, and greatly reducing solvent usage and waste generation essential for onsite analysis.

4.
Environ Sci Technol ; 2019 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-31789509

RESUMO

Fast and effective removal of elemental mercury in a wide temperature range is critical for the smelting industry. In this work, a recyclable magnetic iron sulfide/selenide sorbent is developed to capture and recover Hg0 from smelting flue gas. Benefiting from Se doping, the Hg0 capture performance of prepared FeSxSey is significantly enhanced compared with traditional iron sulfide, especially at high temperatures. Considering the recyclability and working temperature, FeS1.32Se0.11 exhibits the best Hg0 capture performance. The average capture rate of FeS1.32Se0.11 is 3.661 µg/g/min at 80 °C and its saturation adsorption capacity is 20.216 mg/g. The flue gas compositions have almost no effect on Hg0 capture. X-ray photoelectron spectroscopy and mercury thermal programmed desorption suggest that the stable active Se-Sn2- adsorption site can combine with Hg0 to form HgSe, consequently improving Hg0 capture performance at high temperatures. After Hg0 capture, the spent FeSxSey can be collected by magnetic separation and regenerated through selective extraction, which facilitates harmless treatment and resource reuse of mercury. With the advantages of excellent Hg0 capture performance, wide operating temperature range, and remarkable recycling property, FeSxSey microparticles may be a promising sorbent for Hg0 capture in industrial applications, while opening a new avenue to realize the resource utilization toward toxic elements.

5.
Commun Biol ; 2: 390, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31667364

RESUMO

Adolescent idiopathic scoliosis is the most common spinal disorder in adolescents with a prevalence of 0.5-5.2% worldwide. The traditional methods for scoliosis screening are easily accessible but require unnecessary referrals and radiography exposure due to their low positive predictive values. The application of deep learning algorithms has the potential to reduce unnecessary referrals and costs in scoliosis screening. Here, we developed and validated deep learning algorithms for automated scoliosis screening using unclothed back images. The accuracies of the algorithms were superior to those of human specialists in detecting scoliosis, detecting cases with a curve ≥20°, and severity grading for both binary classifications and the four-class classification. Our approach can be potentially applied in routine scoliosis screening and periodic follow-ups of pretreatment cases without radiation exposure.

6.
Biomed Pharmacother ; 120: 109381, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31542616

RESUMO

BACKGROUND: Qinghuo Rougan Formula (QHRGF) is a traditional Chinese medicine (TCM) that has been widely apllied to treat uveitis for several decades. However, the inhibitory mechanism of QHRGF in uveitis has remained to be an enigma. METHODS: The Chinese herbal medicine pharmacology data and analysis platform wereused to search and screen for the effective components of the QHRGF compound injection and to analyse possible therapeutic targets based on network topology. In addition, various known disease target databases were enraolled, the therapeutic target proteins in uveitis were screened, and a protein-protein interaction (PPI) network was constructed. Enrichment analysis was performed on key nodes. Finally, the inhibitory effect of QHRGF on uveitis was verified by experiments. RESULTS: We identified 259 major candidate targets of QHRGF and successfully constructed a 'QHRGF-compound-target-uveitis' network. Above-mentioned targets revealed by Gene enrichment analysis have played an significant role in the cell cycle, autoimmune disease, apoptosis and related signal pathways. We demonstrated that QHRGF attenuates local inflammation in experimental autoimmune uveoretinitis (EAU) rats by regulating natural killer T (NKT) cells and inhibiting MAPK signal pathways. CONCLUSION: QHRGF may regulate the local immune response and inflammatory factors mainly through the MAPK signal pathway. For autoimmune uveitis, QHRGF may be a promising, long-lasting treatment strategy.

7.
J Biochem Mol Toxicol ; 33(10): e22387, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31476248

RESUMO

Breast cancer is a prevalent of tumoregenesis in women and reports for the maximum mortality and morbidity in the global. Ginger (Zingiber officinale) is the mainly widespread spice and herbal remedies used in the world. Since antique periods, ginger has been used in Greece, India and China for the curing of upset stomach, nausea, diarrhea, colds, and headaches. The current work was planned to explore the anticancer properties of zingerone (ZO) toward 7,12-dimethylbenz(a)anthracene (DMBA)-treated mammary carcinogenesis in Sprague-Dawley (SD) rats and MCF-7 mammary cancer cells. The mammary carcinogenesis was produced through a single dosage of DMBA (20 mg/kg bwt) mixed in soya oil (1 mL) administrated intragastrically with a gavage. We found improved concentrations of lipid peroxidation (LOOH and TBARS), carcinoembryonic antigen, lowered levels of enzymatic (CAT, GPx, and SOD), and nonenzymatic (vitamin E, GSH, and vitamin C) antioxidant in mammary tissues and plasma of DMBA-induced cancer bearing animals. Moreover, augmented concentrations of phase I (Cyt-b5 and CYP450 ) and reduced levels of phase II (GR and GST) detoxification microsomal proteins in mammary tissues were noticed. ZO administrations significantly reverted back to all these parameters in this way, showing efficient of anticancer effect. Furthermore, our in vitro study also supported the anticancer effect of the treatment of ZO were noticed loss of cell viability, improved reactive oxygen species formation, and reduced MMP. Furthermore, the status of apoptosis proteins such as Bcl-2, Bax, and Bid expressions was determined by using Western blot analysis techniques. Overall, these results proposed the anticancer effect of ZO toward DMBA-induced mammary cancer in SD animals and Michigan cancer foundation-7 mammary cancer cells.


Assuntos
9,10-Dimetil-1,2-benzantraceno/toxicidade , Apoptose/efeitos dos fármacos , Carcinógenos/farmacologia , Caspases/metabolismo , Guaiacol/análogos & derivados , Neoplasias Mamárias Experimentais/prevenção & controle , Animais , Biotransformação , Western Blotting , Guaiacol/farmacologia , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Células MCF-7 , Neoplasias Mamárias Experimentais/induzido quimicamente , Ratos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo
8.
Polymers (Basel) ; 11(8)2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31382443

RESUMO

Novel proton exchange membranes (PEMs) based on graft copoly(arylene ether sulfone)s with enhanced phase-separated morphology were prepared using atom transfer radical polymerization (ATRP). A series of PEMs with different graft lengths and sulfonation degrees were prepared. The phase-separated morphologies were confirmed by transmission electron microscopy. Among the membranes prepared and evaluated, PAESPS18S2 exhibited considerably high proton conductivity (0.151 S/cm, 85 °C), benefitting from the graft polymer architecture and phase-separated morphology. The membranes also possessed excellent thermal and chemical stabilities. Highly conductive and stable copoly(arylene ether sulfone)-based membranes would be promising candidates as polymer electrolytes for fuel cell applications.

9.
J Hazard Mater ; 380: 120814, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31325696

RESUMO

Although coupling reduced graphene oxide (RGO) with TiO2 is believed to enhance the photocatalysis through the light utilization, studies on its photothermal conversion effect are rarely reported. Herein, RGOP (reduced graphene oxide/P25) was synthesized to explore roles of the enhanced light adsorption and photothermal conversion in the photocatalytic process. It was found that although RGOP had increased absorbance, it actually possessed lower available light utilization compared with P25. In the synergistic effect of available light utilization, transfer resistance and hydrophilicity, RGOP exhibited less superoxide radicals but more hydroxyl radicals. In the presence of scavenger experiments, O2- was proved to play the predominant role in the photocatalytic process, while OH was the secondary one. In comparison to P25, the change of active radicals of RGOP was adverse to its photocatalysis. However, due to the superior adsorptive property of RGOP, it exhibited higher photocatalytic activity than P25. The improved photocatalytic activity of RGOP was ascribed to its superior adsorptive ability aside from active radicals (O2-, OH).

10.
Med Sci Monit ; 25: 5453-5464, 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31332157

RESUMO

BACKGROUND Previous reports suggested that methamphetamine (METH) exposure could lead to inhibition of rat testis spermatogenesis. Glycolysis and glucose metabolism as well as oxidative stress have been implicated in testis spermatogenesis. Here we explored the underlying mechanism of local metabolism and glycolysis of testis after METH exposure. MATERIAL AND METHODS METH was intraperitoneally injected into rats with different doses and duration of METH exposure to establish short-term and chronic exposure models. The serum 8-hydroxy-2 deoxyguanosine (8-OHdG) level of rats was detected by enzyme-linked immunosorbent assay. Untargeted gas chromatography-mass spectrometry analysis was applied to identify differential metabolites and metabolic signature. The mRNA expression of hypoxia inducible factor 1alpha (HIF1alpha), glucose transporter 1 (GLUT1), hexokinase 1 (HK1) and lactate dehydrogenase C (LDHC) in rat testes were detected by polymerase chain reaction. Further, we determined the 4 proteins with western blotting and immunohistochemistry. RESULTS Decreased testes index and sperm counts were showed in the chronic METH group. The metabolome revealed that the main differential metabolites impacted were associated with glycolysis and glucose metabolism. The mRNA and protein expression of GLUT1, HK1, and LDHC were reduced in the chronic METH group but elevated in the short-term METH group, whereas HIF1alpha was upregulated in the short-term METH group but remained at baseline in the chronic METH group. CONCLUSIONS Overall, glucose metabolism was regulated by HIF1alpha after short-term METH exposure. Reduced glycolysis in the testis led to impaired spermatogenesis after chronic METH exposure.

11.
ChemSusChem ; 12(8): 1576-1590, 2019 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-30656828

RESUMO

Chemical energy conversion/storage through water splitting for hydrogen production has been recognized as the ideal solution to the transient nature of renewable energy sources. Solid polymer electrolyte (SPE) water electrolysis is one of the most practical ways to produce pure H2 . Electrocatalysts are key materials in the SPE water electrolysis. At the anode side, electrode materials catalyzing the oxygen evolution reaction (OER) require specific properties. Among the reported materials, only iridium presents high activity and is more stable. In this Minireview, an application overview of single iridium metal and its oxide catalysts-binary, ternary, and multicomponent catalysts of iridium oxides and supported composite catalysts-for the OER in SPE water electrolysis is presented. Two main strategies to improve the activity of an electrocatalyst system, namely, increasing the number of active sites and the intrinsic activity of each active site, are reviewed with detailed examples. The challenges and perspectives in this field are also discussed.

12.
Environ Sci Pollut Res Int ; 26(7): 6735-6744, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30632039

RESUMO

Nonferrous metal smelting produces a large amount of Hg0 in flue gas, which has caused serious damage to the environment and human health. In this work, amorphous cobalt sulfide was synthesized by a liquid-phase precipitation method and was used for capturing gaseous Hg0 from simulated smelting flue gas at low temperatures (50~150 °C). In the adsorption process, Hg0 can be transformed into the stable mercury compound, which is confirmed to be HgS by X-ray photoelectron spectroscopy (XPS) and temperature programmed desorption of Hg (Hg-TPD) analysis. Meanwhile, XPS results also demonstrate that S22- species on the surface of cobalt sulfide play an important role in Hg0 transformation. At the temperature of 50 °C (inlet Hg0 concentration of 214 µg·m-3), the Hg0 adsorption capacity of cobalt sulfide (penetration rate of 25%) is as high as 2.07 mg·g-1, which is much higher than that of popular adsorbents such as activated carbons and metal oxides. In addition, it was found that the Hg0 removal efficiency by cobalt sulfide in the flue gas with high concentration of SO2 (5%) remained more than 94%. The good adsorption and Hg0 removal performance guarantee cobalt sulfide the great superiority and application potential in the treatment of Hg0 in smelting flue gas with high concentration of SO2.


Assuntos
Poluentes Atmosféricos/química , Poluição do Ar/prevenção & controle , Cobalto/química , Mercúrio/química , Metalurgia , Adsorção , Poluentes Atmosféricos/análise , Carvão Vegetal , Gases , Mercúrio/análise , Metais , Óxidos , Espectroscopia Fotoeletrônica , Temperatura Ambiente
13.
J Hazard Mater ; 363: 179-186, 2019 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-30308356

RESUMO

The mercury resources recovery and safe disposal of mercury-containing waste is an urgent problem. In this study, a new method using an iodide solution system was proposed to selectively recover mercury from high mercury-containing smelting wastes. The mercury leaching efficiency, yields, leaching kinetics and thermodynamics were researched. The major factors which affect mercury leaching efficiency including iodide concentration, oxidant, pH and temperature were evaluated. Over 97% and 93% of mercury can be efficiently leached from wastewater treatment sludge (W-S) and acid sludge (A-S). After leaching, the mercury concentration during leaching toxicity test is under the limits set for hazardous waste. Additionally, the electrolytic technology can efficiently recover mercury from leachate in the form of elemental mercury, and the leachate after electrolytic can be reused for mercury leaching. The mercury leaching kinetics follows the shrinking core diffusion model and is controlled by solid product diffusion. The mechanism research shows the leaching efficiency was strongly dependent on the distribution of mercury species in smelting waste. The consequence on mercury leaching and recovery could provide nonferrous smelters with a practical and yet easy-to-adopt perspective to reduce the risk of mercury contamination and selectively recover mercury resources from mercury-containing smelting wastes.

15.
Environ Sci Technol ; 52(20): 11612-11620, 2018 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-30232878

RESUMO

Secondary organic aerosol (SOA), a dominant air pollutant in many countries, threatens the lives of millions of people. Extensive efforts have been invested in studying the formation mechanisms and influence factors of SOA. As promising materials in eliminating air pollutants, the role of photocatalytic materials in SOA formation is unclear. In this study, TiO2 was employed to explore its impact on SOA formation during the photooxidation of m-xylene with NO x in a smog chamber. We found that the presence of TiO2 strongly suppressed SOA formation. The yields of SOA in the photooxidation experiments of m-xylene with NO x were 0.3-4%, whereas negligible SOA was formed when TiO2 was added. When ((NH4)2SO4) was introduced as seed particles, the presence of TiO2 decreased the yields of SOA from 0.3-6% to 0.3-1.6%. The sharply decreased concentrations of reactive carbonyl compounds were the direct cause of the suppression effect of TiO2 on SOA formation. However, the suppression effect was influenced by the addition of seed particles and the initial concentration of NO x. Reaction mechanisms of the photocatalysis of m-xylene with and without NO x were proposed.


Assuntos
Poluentes Atmosféricos , Aerossóis , Compostos Orgânicos , Titânio
16.
J Hazard Mater ; 352: 172-181, 2018 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-29609149

RESUMO

Mechanochemical degradation (MCD) is a promising eco-friendly method to dispose persistent organic pollutants (POPs). Mechanically induced free-radical attack was thought to be one of the key elements in initiating and accelerating the dechlorination and degradation of POPs. In this study, mechanochemical formation of free-radicals and their roles in the remediation of hexachlorobenzene (HCB) contaminated soil were explored using both of experimental analysis and quantum chemical calculations. It was found that chlorinated phenoxy radicals(CB-O) can be produced in the milling process and they played a vital role in the dechlorination of HCB, based on the results of electron spin-resonance (ESR) and X-ray photoelectron spectra (XPS). Two transition states of mechanochemical reaction along the formation of pentachlorinated phenoxy radical (PeCB-O) were located, with the energy barriers of 39.4 and 3.4 kJ/mol. The localized orbital locator (LOL), Mayer bond order and topological analysis were also implemented to depict the process in detail. Free-radical attack dominated dechlorination pathway of HCB in the MCD process was also verified by the Fukui function analysis. The study on the mechanically-induced generation of free-radicals and their associated modes of action on the degradation of HCB will provide a deep insight into mechanochemical remediation mechanism of POPs contaminated soil.

17.
IEEE Trans Neural Syst Rehabil Eng ; 26(3): 698-708, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29522413

RESUMO

In off-line training of motor imagery-based brain-computer interfaces (BCIs), to enhance the generalization performance of the learned classifier, the local information contained in test data could be used to improve the performance of motor imagery as well. Further considering that the covariance matrices of electroencephalogram (EEG) signal lie on Riemannian manifold, in this paper, we construct a Riemannian graph to incorporate the information of training and test data into processing. The adjacency and weight in Riemannian graph are determined by the geodesic distance of Riemannian manifold. Then, a new graph embedding algorithm, called bilinear regularized locality preserving (BRLP), is derived upon the Riemannian graph for addressing the problems of high dimensionality frequently arising in BCIs. With a proposed regularization term encoding prior information of EEG channels, the BRLP could obtain more robust performance. Finally, an efficient classification algorithm based on extreme learning machine is proposed to perform on the tangent space of learned embedding. Experimental evaluations on the BCI competition and in-house data sets reveal that the proposed algorithms could obtain significantly higher performance than many competition algorithms after using same filter process.


Assuntos
Algoritmos , Interfaces Cérebro-Computador , Imaginação , Movimento , Eletroencefalografia/classificação , Eletroencefalografia/métodos , Humanos , Aprendizagem , Aprendizado de Máquina , Reprodutibilidade dos Testes
18.
ACS Omega ; 3(4): 4070-4080, 2018 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31458643

RESUMO

The extraction of lanthanide series by Cyanex301, i.e., bis(2,4,4-trimethylpentyl)dithiophosphinic acid (HC301), has been modeled by density functional theory calculation, taking into account the formation of both inner- and outer-sphere complexes. The inner-sphere complex Ln(C301)3 and the outer-sphere complex Ln(H2O)9(C301)3 are optimized, followed by the analysis of interaction energy, bond length, Laplacian bond orders, and Mulliken populations. The covalency degree increases in Ln-S and Ln-O bonds in the inner- and outer-sphere complexes, respectively, as the lanthanide series is traversed. Mulliken population analysis indicates the important role of the 5d-orbital participation in bonding in the formation of inner- and outer-sphere complexes. Two thermodynamic cycles regarding the formation of inner- and outer-sphere complexes are established to calculate the extraction Gibbs free energies (ΔG extr), and relaxed potential energy surface scan is utilized to model the kinetic complexation of C301 anion with hydrated metal ions. Light lanthanides can form both inner- and outer-sphere complexes, whereas heavy lanthanides only form outer-sphere complexes in biphasic extraction. After adopting the data of forming inner-sphere complex for light Ln(III) and that of forming outer-sphere complexes for heavy Ln(III), the trend of the calculated -ΔG extr agrees very well with that of the experimental distribution ratios on crossing the Ln(III) series. Results from this work help to theoretically understand the extraction behavior of Cyanex301 with respect to different Ln(III).

19.
J Evid Based Complementary Altern Med ; 22(4): 883-891, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-29228810

RESUMO

Many studies have investigated the efficacy of Endostar combined with transcatheter arterial chemoembolization (TACE) versus TACE alone for hepatocellular carcinoma (HCC). A systematic review was conducted to evaluate the efficacy of Endostar. PubMed, Embase, and other databases were searched, and meta-analysis was performed using RevMan 5.3 software. Nine studies, all of which were clinical randomized controlled trials, involving 411 participants were included. The overall response rate, disease control rate and α-fetoprotein negative conversion ratio, and the 6- and 12-month survival rate of HCC patients treated with combined Endostar and TACE were higher than those treated with TACE alone ( P < .01). Furthermore, the incidence of tumor progression was low after Endostar treatment ( P = .005). The incidence of adverse effects (leukocytopenia, liver function damage, and vomiting) was similar in Endostar with TACE and in TACE alone ( P > .05). However, large studies and more randomized trials are necessary to determine the effects of Endostar on HCC.


Assuntos
Inibidores da Angiogênese/uso terapêutico , Carcinoma Hepatocelular/terapia , Quimioembolização Terapêutica/métodos , Endostatinas/uso terapêutico , Neoplasias Hepáticas/terapia , Proteínas Recombinantes/uso terapêutico , Carcinoma Hepatocelular/mortalidade , Quimioembolização Terapêutica/efeitos adversos , Terapia Combinada , Endostatinas/efeitos adversos , Humanos , Neoplasias Hepáticas/mortalidade , Proteínas Recombinantes/efeitos adversos
20.
PLoS One ; 12(9): e0184669, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28910353

RESUMO

BACKGROUND: The six-minute walk test (6MWT) is a safe, simple, inexpensive tool for evaluating the functional exercise capacity of patients with chronic respiratory disease. However, there is a lack of standard reference equations for the six-minute walk distance (6MWD) in the healthy Chinese population aged 18-59 years. AIMS: The purposes of the present study were as follows: 1) to measure the anthropometric data and walking distance of a sample of healthy Chinese Han people aged 18-59 years; 2) to construct reference equations for the 6MWD; 3) to compare the measured 6MWD with previously published equations. METHOD: The anthropometric data, demographic information, lung function, and walking distance of Chinese adults aged 18-59 years were prospectively measured using a standardized protocol. We obtained verbal consent from all the subjects before the test, and the study design was approved by the ethics committee of Wenzhou People's Hospital. The 6MWT was performed twice, and the longer distance was used for further analysis. RESULTS: A total of 643 subjects (319 females and 324 males) completed the 6MWT, and average walking distance was 601.6±55.51 m. The walking distance was compared between females and males (578±49.85 m vs. 623±52.53 m; p < 0.0001) and between physically active subjects and sedentary subjects (609.3±56.17 m vs. 592±53.23 m; p < 0.0001). Pearson's correlation indicated that the 6MWD was significantly correlated with various demographic and the 6MWT variables, such as age, height, weight, body mass index (BMI), heart rate after the test and the difference in the heart rate before and after the test. Stepwise multiple regression analysis showed that age and height were independent predictors associated with the 6MWD. The reference equations from white, Canadian and Chilean populations tended to overestimate the walking distance in our subjects, while Brazilian and Arabian equations tended to underestimate the walking distance. There was no significant difference in the walking distance between Korean reference equations and the results of the current study. CONCLUSION: In summary, age and height were the most significant predictors of the 6MWD, and regression equations could explain approximately 34% and 28% of the distance variance in the female and male groups, respectively.


Assuntos
Teste de Esforço/métodos , Teste de Esforço/normas , Caminhada/fisiologia , Adulto , Fatores Etários , Antropometria , Composição Corporal , Estatura , China , Estudos Transversais , Feminino , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Padrões de Referência , Valores de Referência , Análise de Regressão , Adulto Jovem
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