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1.
Gene ; 764: 145080, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32858178

RESUMO

Spermatocyte (spc) formation from spermatogonia (spg) differentiation is the first step of spermatogenesis which produces prodigious spermatozoa for a lifetime. After decades of studies, several factors involved in the functioning of a mouse were discovered both inside and outside spg. Considering the peculiar expression and working pattern of each factor, this review divides the whole conversion of spg to spc into four consecutive development processes with a focus on extracellular cues and downstream transcription network in each one. Potential coordination among Dmrt1, Sohlh1/2 and BMP families mediates Ngn3 upregulation, which marks progenitor spg, with other changes. After that, retinoic acid (RA), as a master regulator, promotes A1 spg formation with its helpers and Sall4. A1-to-B spg transition is under the control of Kitl and impulsive RA signaling together with early and late transcription factors Stra8 and Dmrt6. Finally, RA and its responsive effectors conduct the entry into meiosis. The systematic transcription network from outside to inside still needs research to supplement or settle the controversials in each process. As a step further ahead, this review provides possible drug targets for infertility therapy by cross-linking humans and mouse model.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Espermatócitos/fisiologia , Espermatogênese/genética , Espermatogônias/fisiologia , Animais , Proteínas Morfogenéticas Ósseas/metabolismo , Autorrenovação Celular/genética , Humanos , Masculino , Camundongos , Túbulos Seminíferos/citologia , Túbulos Seminíferos/crescimento & desenvolvimento , Fatores de Transcrição/metabolismo , Transcrição Genética , Tretinoína/metabolismo , Regulação para Cima
2.
J Neuroinflammation ; 17(1): 330, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33153475

RESUMO

BACKGROUND: Inflammatory response has been recognized as a pivotal pathophysiological process during cerebral ischemic stroke. NLRP3 inflammasome, involved in the regulation of inflammatory cascade, can simultaneously lead to GSDMD-executed pyroptosis in cerebral ischemia. Low-density lipoprotein receptor (LDLR), responsible for cholesterol uptake, was noted to exert potential anti-inflammatory bioactivities. Nevertheless, the role of LDLR in neuroinflammation mobilized by cerebral ischemia/reperfusion (I/R) has not been investigated. METHODS: Ischemic stroke mice model was accomplished by middle cerebral artery occlusion. Oxygen-glucose deprivation was employed after primary cortical neuron was extracted and cultured. A pharmacological inhibitor of NLRP3 (CY-09) was administered to suppress NLPR3 activation. Histological and biochemical analysis were performed to assess the neuronal death both in vitro and in vivo. In addition, neurological deficits and behavioral deterioration were evaluated in mice. RESULTS: The expression of LDLR was downregulated following cerebral I/R injury. Genetic knockout of Ldlr enhanced caspase-1-dependent cleavage of GSDMD and resulted in severe neuronal pyroptosis. LDLR deficiency contributed to excessive NLRP3-mediated maturation and release of IL-1ß and IL-18 under in vitro and in vivo ischemic conditions. These influences ultimately led to aggravated neurological deficits and long-term cognitive dysfunction. Blockade of NLRP3 substantially retarded neuronal pyroptosis in Ldlr-/- mice and cultured Ldlr-/- neuron after experimental stroke. CONCLUSIONS: These results demonstrated that LDLR modulates NLRP3-mediated neuronal pyroptosis and neuroinflammation following ischemic stroke. Our findings characterize a novel role for LDLR as a potential therapeutic target in neuroinflammatory responses to acute cerebral ischemic injury.

3.
Environ Pollut ; : 115930, 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33183869

RESUMO

Advanced oxidation processes (AOPs) based on peroxymonosulfate (PMS) activation have attracted increasing attention in recent years for organic pollutants removal. Herein, we put forward a facile method to form cobalt phosphide/carbon composite for PMS activation. Combining impregnation approach with pyrolysis treatment enabled the formation of Co2P/biochar composites using baker's yeast and Co2+ as precursors. The as-synthesized products exhibited excellent catalytic activity for sulfamethoxazole (SMX) degradation over the pH range 3.0-9.0 b y activating PMS. For example, 100% of SMX (20 mg L-1) removal was achieved in 20 min with catalyst dosage of 0.4 g L-1 and PMS loading of 0.4 g L-1. Near zero Co2+ leaching was observed during catalytic reaction, which remarkably lowered the toxic risk of transition metal ion in water. Meanwhile, the reusability of catalyst could be attained by thermal treatment. SMX degradation intermediates were identified by liquid chromatography-mass spectrometry (LC-MS), which facilitated the proposal of possible SMX degradation pathways. Ecological Structure Activity Relationships (ECOSAR) analysis indicated that SMX degradation intermediates may not pose ecological toxicity to the environment. Further investigation verified that Co2P/biochar composites could set off PMS activation not only for the degradation of SMX but also for other sulfonamides. In this study, we not only developed a facile method of utilizing environmental-benign biomass for transition metal phosphide/carbon composite formation, but also achieved highly efficient antibiotic elimination by PMS-based AOP.

4.
J Clin Invest ; 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33141760

RESUMO

Oligodendrocytes express low-density lipoprotein receptor (LDLR) to endocytose cholesterol for the maintenance of adulthood myelination. However, the potential role of LDLR in chronic cerebral ischemia-related demyelination remains unclear. We used bilateral carotid artery stenosis (BCAS) to induce sustained cerebral ischemia in mice. This hypoxic-ischemic injury caused a remarkable decline of oligodendroglial LDLR with impaired oligodendroglial differentiation and survival. Oligodendroglial cholesterol levels, however, remained unchanged. Mice miR-344e-3p and human homolog miR-410-3p, two miRNAs directly targeting Ldlr, were identified in experimental and clinical leukoaraiosis, thus leading to LDLR reduction. Lentiviral delivery of LDLR ameliorated the demyelination following chronic cerebral ischemia. By contrast, Ldlr-/- mice displayed inadequate myelination in the corpus callosum. Ldlr-/- oligodendrocyte progenitor cells (OPCs) exhibited defective ability to differentiate and myelinate axons in vitro. Transplantation with Ldlr-/- OPCs could not rescue the BCAS-induced demyelination. Such LDLR-dependent myelin restoration might involve a physical interaction of the Asn-Pro-Val-Tyr (NPVY) motif with phosphotyrosine binding domain of Shc, which subsequently activated MEK/ERK pathway. Together, our findings demonstrate that the aberrant oligodendroglial LDLR in chronic cerebral ischemia impairs myelination through intracellular signal transduction. Preservation of oligodendroglial LDLR may provide a promising approach to treat ischemic demyelination.

5.
JMIR Mhealth Uhealth ; 8(11): e18907, 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33164904

RESUMO

BACKGROUND: With the rise of mobile medicine, the development of new technologies such as smart sensing, and the popularization of personalized health concepts, the field of smart wearable devices has developed rapidly in recent years. Among them, medical wearable devices have become one of the most promising fields. These intelligent devices not only assist people in pursuing a healthier lifestyle but also provide a constant stream of health care data for disease diagnosis and treatment by actively recording physiological parameters and tracking metabolic status. Therefore, wearable medical devices have the potential to become a mainstay of the future mobile medical market. OBJECTIVE: Although previous reviews have discussed consumer trends in wearable electronics and the application of wearable technology in recreational and sporting activities, data on broad clinical usefulness are lacking. We aimed to review the current application of wearable devices in health care while highlighting shortcomings for further research. In addition to daily health and safety monitoring, the focus of our work was mainly on the use of wearable devices in clinical practice. METHODS: We conducted a narrative review of the use of wearable devices in health care settings by searching papers in PubMed, EMBASE, Scopus, and the Cochrane Library published since October 2015. Potentially relevant papers were then compared to determine their relevance and reviewed independently for inclusion. RESULTS: A total of 82 relevant papers drawn from 960 papers on the subject of wearable devices in health care settings were qualitatively analyzed, and the information was synthesized. Our review shows that the wearable medical devices developed so far have been designed for use on all parts of the human body, including the head, limbs, and torso. These devices can be classified into 4 application areas: (1) health and safety monitoring, (2) chronic disease management, (3) disease diagnosis and treatment, and (4) rehabilitation. However, the wearable medical device industry currently faces several important limitations that prevent further use of wearable technology in medical practice, such as difficulties in achieving user-friendly solutions, security and privacy concerns, the lack of industry standards, and various technical bottlenecks. CONCLUSIONS: We predict that with the development of science and technology and the popularization of personalized health concepts, wearable devices will play a greater role in the field of health care and become better integrated into people's daily lives. However, more research is needed to explore further applications of wearable devices in the medical field. We hope that this review can provide a useful reference for the development of wearable medical devices.

7.
J Hazard Mater ; : 124273, 2020 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-33131939

RESUMO

In the field of radioactive waste immobilization, the investigation of irradiation stability is of considerable importance. In this study, uranium-contaminated soil samples were irradiated by 1.5 MeV Xe20+ ions with fluences ranging from 1 × 1012 to 1 × 1015 ions/cm2. Xe20+ heavy-ion radiation was used to simulate the self-irradiation of actinide nuclides. The uranium-contaminated soil samples were sintered via microwaves. Grazing incidence X-ray diffraction results showed that irradiation can cause crystallization of the sample. After irradiation, the Vickers hardness of the samples decreased slightly. Comparative analysis showed that the sample had good radiation resistance, and the leaching rate (28 d) of the sample increased slightly after irradiation, but the overall performance was stable. Our investigation reveals the corresponding mechanism of uranium-contaminated soil irradiation of 1.5 MeV Xe20+ ions.

8.
Mol Med Rep ; 22(6): 5472-5478, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33174004

RESUMO

Although insulin is known to affect neointimal hyperplasia via distinct signaling pathways, how neointimal hyperplasia is affected in insulin­deficient type 1 diabetes remains unknown. The aim of the current study was to investigate two major signaling branches of insulin action regulating neointimal hyperplasia following arterial injury in type 1 diabetes with or without exogenous insulin administration. Rats were treated with vehicle (control group), streptozotocin (STZ) alone (STZ group; uncontrolled type 1 diabetes) or STZ followed by insulin (STZ + I group; controlled type 1 diabetes). Subsequently, a type 1 diabetic rat model of carotid artery balloon injury was established. Following this, the intima­to­media area ratios were examined for evidence of neointimal hyperplasia in the carotid arteries of the rats by performing hematoxylin­eosin staining. Furthermore, the protein expression of extracellular signal­regulated kinase (ERK), phosphorylated (p­) ERK, protein kinase B (Akt) and p­Akt in the carotid arteries of the rats was determined via immunoblotting. Moreover, an in vitro model of type 1 diabetes was induced by incubation of primary vascular smooth muscle cells (VSMCs) with glucose and/or insulin. Cellular proliferation and signaling protein expression levels in VSMCs were determined by measuring the incorporation of tritiated thymidine and performing immunoblotting, respectively. The results demonstrated that compared with that in control rats, neointimal hyperplasia and expression of p­Akt in uncontrolled type 1 diabetic rats were significantly decreased. This decrease was recovered in controlled type 1 diabetes with insulin therapy. Furthermore, the difference in the expression of p­ERK between groups was not significant. Additionally, the results of the cell experiments were consistent with those from the animal studies. In conclusion, the preferential signaling along the phosphatidylinositol 3­kinase/Akt pathway of insulin action in response to insulin restoration may contribute to neointimal hyperplasia. The present study provides a novel approach for the further treatment of neointimal hyperplasia in type 1 diabetes.

9.
Nanoscale ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33201980

RESUMO

We investigate the geometric and electronic properties of single-atom catalysts (SACs) within metal-organic frameworks (MOFs) with respect to electrocatalytic CO2 reduction as a model reaction. A series of mid-to-late 3d transition metals have been immobilised within the microporous cavity of UiO-66-NH2. By employing Rietveld refinement of new-generation synchrotron diffraction, we not only identified the crystallographic and atomic parameters of the SACs that are stabilised with a robust MN(MOF) bonding of ca. 2.0 Å, but also elucidated the end-on coordination geometry with CO2. A volcano trend in the FEs of CO has been observed. In particular, the confinement effect within the rigid MOF can greatly facilitate redox hopping between the Cu SACs, rendering high FEs of CH4 and C2H4 at a current density of -100 mA cm-2. Although only demonstrated in selected SACs within UiO-66-NH2, this study sheds light on the rational engineering of molecular interactions(s) with SACs for the sustainable provision of fine chemicals.

10.
Life Sci ; 263: 118586, 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33065148

RESUMO

AIMS: Mitochondrial dysfunction is receiving considerable attention due to irreplaceable biological function of mitochondria. Ionizing radiation and tigecycline (TIG) alone can cause mitochondrial dysfunction, playing important role in tumor therapy. However, prior studies fail to investigate combined mechanism of carbon ion irradiation (IR) and TIG on tumor proliferation inhibition. The study aimed to explore the combined effects of both on autophagy and apoptosis. MATERIALS AND METHODS: NSCLC cells A549 and H1299 were treated with carbon ion, TIG, or both. Cell survival rate, autophagy, apoptosis, expression of mitochondrial signaling proteins were determined by clone formation assay, immunofluorescence of LC3B, flow cytometry and western blotting, respectively; ATP content, mitochondrial membrane potential (MMP) and Ca2+ level in mitochondria were used to assessed mitochondrial function. KEY FINDINGS: Results showed IR combined TIG inhibited cells proliferation by increasing apoptosis in both cells and enhancing autophagy in H1299 cells. Additionally, combination treatment induced the most severe mitochondrial dysfunction by sharply reducing ATP, MMP and increasing Ca2+ level of mitochondria. Up-regulation and down-regulation of mitochondrial translation proteins (EF-Tu, GFM1 and MRPS12) expression affected apoptosis and autophagy, while the level of p-mTOR was consistent with their expression in both cell types. In A549 cells, p-AMPK level decreased while p-Akt and p-mTOR increased after combination treatment. SIGNIFICANCE: Overall, our results showed that p-Akt and p-AMPK antagonistically targeted p-mTOR to regulate mitochondrial translation proteins to affect autophagy and apoptosis. Furthermore, this study suggests that combination of carbon ion and TIG is a potential therapeutic option against tumors.

11.
J Vis Exp ; (163)2020 09 22.
Artigo em Inglês | MEDLINE | ID: mdl-33044454

RESUMO

Sleep disturbance is generally common in populations as a chronic disease or a complained event. Chronic sleep disturbance is proposed to be closely linked to the pathogenesis of diseases, especially neurodegenerative diseases. We recently found that 2 months of sleep fragmentation initiated Alzheimer's disease (AD)-like behavioral and pathological changes in young wild-type mice. Herein, we present a standardized protocol to achieve chronic sleep fragmentation (CSF). Briefly, CSF was induced by an orbital rotor vibrating at 110 rpm and operating with a repetitive cycle of 10 s-on, 110 s-off, during light-ON phase (8:00 AM-8:00 PM) continuously for up to 2 months. Impairments of spatial learning and memory, anxiety-like but not depression-like behavior in mice as consequences of CSF modeling, were evaluated with Morris water maze (MWM), Novel object recognition (NOR), Open field test (OFT) and Forced swimming test (FST). In comparison with other sleep manipulations, this protocol minimizes the handling labors and maximizes the modeling efficiency. It produces stable phenotypes in young wild-type mice and can be potentially generated for a variety of research purposes.

12.
J Bus Res ; 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-33110278

RESUMO

Despite their ubiquity in academic and commercial research, evidence of the usefulness of consumer confidence indices is mixed. To contribute to this debate, we examine the psychological mechanisms through which consumer confidence does (and does not) affect consumer behavior. We develop a conceptual model, which we test via structural equation modelling and moderated mediation analysis, using data from a sample of US consumers (n = 1,090). Rather than conceptualize consumer confidence as a single construct, our study is the first to distinguish between national consumer confidence and personal consumer confidence. Consistent with cognitive appraisal theory, personal consumer confidence mediates the relationship between national consumer confidence and perceived financial vulnerability, which in turn leads to increased price conscious behavior. Drawing on attribution theory, we find that external locus of control enhances the effects of national consumer confidence. We provide practical advice to economic forecasters, business analysts, marketers, and financial educators.

13.
Arch Microbiol ; 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33108487

RESUMO

Bacterial strain BBQ-18T, isolated from a freshwater lake in Taiwan, is characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicate that strain BBQ-18T forms a phylogenetic lineage in the genus Flavobacterium. Strain BBQ-18T is most closely related to Flavobacterium alvei HR-AYT with 98.5% 16S rRNA gene sequence similarity. Strain BBQ-18T shows 70.5-89.5% average nucleotide identity and 13.7-38.2% digital DNA-DNA hybridization identity with the type strains of other closely related Flavobacterium species. The strain is Gram-stain negative, strictly aerobic, motile by gliding, rod shaped and formed yellow colonies. Optimal growth occurs at 25 °C, pH 6, and in the absence of NaCl. Strain BBQ-18T contains iso-C15:0, summed feature 3 (C16:1ω6c and/or C16:1ω7c) and anteiso-C15:0 as the predominant fatty acids. The polar lipid profile consists of phosphatidylethanolamine, four uncharacterized aminophospholipids and two uncharacterized phospholipids. The major polyamine is homospermidine. The major isoprenoid quinone is MK-6. The DNA G+C content of the genomic DNA is 33.8%. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain BBQ-18T should be classified as a novel species of the genus Flavobacterium, for which the name Flavobacterium undicola sp. nov. is proposed. The type strain is BBQ-18T (= BCRC 81050T = LMG 30052T = KCTC 52810T).

14.
Biomed Res Int ; 2020: 6287545, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33062689

RESUMO

An increasing number of patients infected with nontuberculous mycobacteria (NTM) are observed worldwide. However, it is challenging to identify NTM lung diseases from pulmonary tuberculosis (PTB) due to considerable overlap in classic manifestations and clinical and radiographic characteristics. This study quantifies both cavitary and bronchiectasis regions in CT images and explores a machine learning approach for the differentiation of NTM lung diseases and PTB. It involves 116 patients and 103 quantitative features. After the selection of informative features, a linear support vector machine performs disease classification, and simultaneously, discriminative features are recognized. Experimental results indicate that bronchiectasis is relatively more informative, and two features are figured out due to promising prediction performance (area under the curve, 0.84 ± 0.06; accuracy, 0.85 ± 0.06; sensitivity, 0.88 ± 0.07; and specificity, 0.80 ± 0.12). This study provides insight into machine learning-based identification of NTM lung diseases from PTB, and more importantly, it makes early and quick diagnosis of NTM lung diseases possible that can facilitate lung disease management and treatment planning.

15.
Ann Clin Lab Sci ; 50(5): 600-610, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33067206

RESUMO

OBJECTIVE: Tuberculosis (TB) is the most common cause of acquired immune deficiency syndrome (AIDS)-related deaths worldwide. The purpose of this study was to identify genes that are significant for the mechanisms involved in Mycobacterium tuberculosis (MTB) and human immunodeficiency virus (HIV) co-infection. MATERIALS AND METHODS: We selected 113 HIV/TB and 109 HIV/LTBI (latent TB infection) genes from GSE37250 and GSE69581 datasets. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used for Kyoto Encyclopedia of Gene and Genome (KEGG) pathway and gene ontology (GO) analyses. The protein-protein interaction (PPI) network of common differentially expressed genes (DEGs) were visualized using Cytoscape software with Search Tool for the Retrieval of Interacting Genes (STRING). RESULTS: A total of 83 DEGs were found to be common to both datasets. These included 64 up-regulated genes and 19 down-regulated genes. The PPI network was analyzed, and 12 up-regulated genes were identified. Re-analysis using DAVID found no significant signaling pathways enriched by these twelve genes (CAMP, CTSG, DEFA1, DEFA1B, DEFA3, DEFA4, ELANE, HP, HPSE, OLFM4, PGLYRP1, TCN1). CONCLUSIONS: Twelve significantly up-regulated DEGs that may be potential therapeutic targets for HIV/TB were identified using a series of bioinformatics analytical methods.

16.
Adv Mater ; : e2003251, 2020 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-33073405

RESUMO

Metal-air fuel cells with high energy density, eco-friendliness, and low cost bring significantly high security to future power systems. However, the impending challenges of low power density and high-current-density stability limit their widespread applications. In this study, an ultrahigh-power-density Zn-air fuel cell with robust stability is highlighted. Benefiting from the water-resistance effect of the confined nanopores, the highly active cobalt cluster electrocatalysts reside in specific nanopores and possess stable triple-phase reaction areas, leading to the synergistic optimization of electron conduction, oxygen gas diffusion, and ion transport for electrocatalysis. As a result, the as-established Zn-air fuel cell shows the best stability under high-current-density discharging (>90 h at 100 mA cm-2 ) and superior power density (peak power density: >300 mW cm-2 , specific power: 500 Wgcat -1 ) compared to most reported non-noble-metal electrocatalysts. The findings will provide new insights in the rational design of electrocatalysts for advanced metal-air fuel cell systems.

17.
Sci Rep ; 10(1): 15543, 2020 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-32968148

RESUMO

Targeted protein degradation (TPD) has emerged as a powerful tool in drug discovery for the perturbation of protein levels using heterobifunctional small molecules. E3 ligase recruiters remain central to this process yet relatively few have been identified relative to the ~ 600 predicted human E3 ligases. While, initial recruiters have utilized non-covalent chemistry for protein binding, very recently covalent engagement to novel E3's has proven fruitful in TPD application. Herein we demonstrate efficient proteasome-mediated degradation of BRD4 by a bifunctional small molecule linking the KEAP1-Nrf2 activator bardoxolone to a BRD4 inhibitor JQ1.

18.
PLoS One ; 15(9): e0239405, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32997680

RESUMO

Sri Lanka's community forestry (CF) program emerged in the early 1980s following a global trend to conserve forest resources and provide benefits to the local community. However, very little is known about the effect of CF on forest resources. We assess the impacts of CF on forest conditions of semi-mixed evergreen forest in the intermediate zone of Sri Lanka using the before-after control-impact method. The study examines tree density, regeneration, woody species diversity, and evidence of disturbance as parameters to analyze the impact of the CF program. Data are analyzed using the difference in differences approach. The results show that the CF program has increased seedling and sapling density to a significant degree and reduced human disturbances. A major contribution of the CF program is that it was found to reduce invasive species and forest fires. The program reduced the amount of invasive species up to six times less than previous. The findings revealed that the impact of CF on forests may vary depending on pre-existing forest conditions, length of period to implement, perception, and decisions by local people. Community understanding and decision-making, in tandem with government policy, will weigh heavily on its future effectiveness.


Assuntos
Conservação dos Recursos Naturais/métodos , Agricultura Florestal , Florestas , Características de Residência , Biodiversidade , Humanos , Sri Lanka , Árvores/crescimento & desenvolvimento
19.
Can Respir J ; 2020: 1654653, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32963642

RESUMO

Identify the treatment effects and risk factors for mortality in patients with pulmonary tuberculosis receiving antituberculosis treatment under the Directly Observed Treatment Short-Course (DOTS) program to reduce the mortality rate of tuberculosis. A retrospective cohort analysis was conducted on the outcomes of antituberculosis treatment of 7,032 patients with tuberculosis in the DOTS program, in the Tuberculosis Management Information System from 2014 to 2017 in Tianjin, China. The Kaplan-Meier method and multifactor Cox proportional risk regression model were used to analyze the risk factors for mortality during antituberculosis treatment under DOTS. The success rate of antituberculosis treatment was 90.24% and the mortality rate was 4.56% among 7,032 cases of tuberculosis in Tianjin. Cox regression analysis showed that advanced age, male sex, human immunodeficiency virus (HIV) positivity, first sputum positivity, retreated tuberculosis, and a delayed visit (≥14 days) were risk factors for mortality in patients with pulmonary tuberculosis receiving antituberculosis treatment under DOTS. The treatment effects in patients with pulmonary tuberculosis during antituberculosis treatment under DOTS were positive in Tianjin. Advanced age, male sex, HIV positivity, first sputum positivity, retreated tuberculosis, and a delayed visit (≥14 days) increased the risk for mortality during antituberculosis treatment.

20.
Talanta ; 220: 121430, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32928435

RESUMO

A simple and fast method for copper ions (Cu2+) and silver ions (Ag+) detection was established with cadmium telluride quantum dots (CdTe QDs) as fluorescent probes. In the presence of Cu2+ or Ag+, the fluorescence intensity of TGA-CdTe QD can be significantly quenched, which fitted a linear relationship between the fluorescence quenching degree (F0-F)/F0 and the concentration of metal ions. In this work, the lowest detected concentration for Cu2+ and Ag+ was 35.0 nM and 25.3 nM, respectively. In addition, the differentiation of Cu2+ and Ag+ at different concentrations was realized with the principal component analysis (PCA). Furthermore, Cu2+ was successfully detected in body fluids. This method provides a good potential for copper ions and silver ions detection with simplicity, rapidity, and excellent selectivity.

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