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1.
Oxid Med Cell Longev ; 2022: 4564471, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35308167

RESUMO

The polarization of microglia is recognized as a crucial factor in reducing neuroinflammation and promoting hematoma clearance after intracerebral hemorrhage (ICH). Previous studies have revealed that redox components participate in the regulation of microglial polarization. Recently, the novel Nrf2 activator omaveloxolone (Omav) has been validated to improve neurological function in patients with neurodegenerative disorders by regulating antioxidant responses. In this study, we examined the efficacy of Omav in ICH. Omav significantly promoted Nrf2 nuclear accumulation and the expression of HO-1 and NQO1 in BV2 cells. In addition, both in vitro and in vivo experiments showed that Omav treatment inhibited M1-like activation and promoted the activation of the M2-like microglial phenotype. Omav inhibited OxyHb-induced ROS generation and preserved the function of mitochondria in BV2 cells. Intraperitoneal administration of Omav improved sensorimotor function in the ICH mouse model. Importantly, these effects were blocked by pretreatment with ML385, a selective inhibitor of Nrf2. Collectively, Omav modulated microglial polarization by activating Nrf2 and inhibiting ROS generation in ICH models, suggesting that it might be a promising drug candidate for the treatment of ICH.


Assuntos
Lesões Encefálicas , Microglia , Animais , Lesões Encefálicas/tratamento farmacológico , Hemorragia Cerebral/complicações , Hemorragia Cerebral/tratamento farmacológico , Hemorragia Cerebral/metabolismo , Humanos , Camundongos , Microglia/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Fenótipo , Triterpenos
2.
Commun Biol ; 5(1): 913, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36068295

RESUMO

Fundamental and clinical neuroscience has benefited tremendously from the development of automated computational analyses. In excess of 600 human neuroimaging papers using Voxel-based Morphometry (VBM) are now published every year and a number of different automated processing pipelines are used, although it remains to be systematically assessed whether they come up with the same answers. Here we examined variability between four commonly used VBM pipelines in two large brain structural datasets. Spatial similarity and between-pipeline reproducibility of the processed gray matter brain maps were generally low between pipelines. Examination of sex-differences and age-related changes revealed considerable differences between the pipelines in terms of the specific regions identified. Machine learning-based multivariate analyses allowed accurate predictions of sex and age, however accuracy differed between pipelines. Our findings suggest that the choice of pipeline alone leads to considerable variability in brain structural markers which poses a serious challenge for reproducibility and interpretation.


Assuntos
Encéfalo , Imageamento por Ressonância Magnética , Encéfalo/diagnóstico por imagem , Mapeamento Encefálico/métodos , Substância Cinzenta/diagnóstico por imagem , Humanos , Imageamento por Ressonância Magnética/métodos , Reprodutibilidade dos Testes
3.
Am J Lifestyle Med ; 16(5): 622-632, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072688

RESUMO

Objective. This cross-sectional study examined associations between adolescent dietary behaviors and family/home environments with parent-adolescent dyadic analysis. Methods. Secondary data were analyzed for adolescents and their parents who participated in the 2014 Family Life, Activity, Sun, Health, and Eating (FLASHE) study. Relationships between adolescent and parent intake of convenience/fast-food, fruits/vegetables, and sugar, as well as family/community and mealtime environments, were examined using Pearson's correlations, 2-sample t tests, analysis of variance, and general linear model analyses. Results. Among this nationwide parent-adolescent dyad sample (N = 1890), a moderate relationship between parent and adolescent consumption of convenience/fast-foods (r = 0.426) and fruits/vegetables (r = 0.416) was found. Adolescent convenience/fast-foods intake differed by sex, neighborhood socioeconomic status, meals eaten with family, and meals eaten in front of the TV (P < .001 for all) as well as race/ethnicity (P = .004). Adolescent fruit/vegetable intake differed by home location (P = .029), school location (P = .032), meals eaten together with family, meals eaten in front of the TV, and body mass index category (P < .001 for all). Generalized linear models revealed multiple predictors of adolescent dietary behaviors. Conclusion. Findings suggest dynamic relationships between adolescent and parent dietary cancer risk factors and adolescent fruit/vegetable consumption disparities by multiple environmental factors. Interventions targeting adolescent-parent dyads may help reduce cancer risk associated with diet.

4.
Lancet Oncol ; 23(9): 1133-1144, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36055304

RESUMO

BACKGROUND: The first interim analysis of the KEYNOTE-564 study showed improved disease-free survival with adjuvant pembrolizumab compared with placebo after surgery in patients with clear cell renal cell carcinoma at an increased risk of recurrence. The analysis reported here, with an additional 6 months of follow-up, was designed to assess longer-term efficacy and safety of pembrolizumab versus placebo, as well as additional secondary and exploratory endpoints. METHODS: In the multicentre, randomised, double-blind, placebo-controlled, phase 3 KEYNOTE-564 trial, adults aged 18 years or older with clear cell renal cell carcinoma with an increased risk of recurrence were enrolled at 213 hospitals and cancer centres in North America, South America, Europe, Asia, and Australia. Eligible participants had an Eastern Cooperative Oncology Group performance status of 0 or 1, had undergone nephrectomy 12 weeks or less before randomisation, and had not received previous systemic therapy for advanced renal cell carcinoma. Participants were randomly assigned (1:1) via central permuted block randomisation (block size of four) to receive pembrolizumab 200 mg or placebo intravenously every 3 weeks for up to 17 cycles. Randomisation was stratified by metastatic disease status (M0 vs M1), and the M0 group was further stratified by ECOG performance status and geographical region. All participants and investigators involved in study treatment administration were masked to the treatment group assignment. The primary endpoint was disease-free survival by investigator assessment in the intention-to-treat population (all participants randomly assigned to a treatment). Safety was assessed in the safety population, comprising all participants who received at least one dose of pembrolizumab or placebo. As the primary endpoint was met at the first interim analysis, updated data are reported without p values. This study is ongoing, but no longer recruiting, and is registered with ClinicalTrials.gov, NCT03142334. FINDINGS: Between June 30, 2017, and Sept 20, 2019, 994 participants were assigned to receive pembrolizumab (n=496) or placebo (n=498). Median follow-up, defined as the time from randomisation to data cutoff (June 14, 2021), was 30·1 months (IQR 25·7-36·7). Disease-free survival was better with pembrolizumab compared with placebo (HR 0·63 [95% CI 0·50-0·80]). Median disease-free survival was not reached in either group. The most common all-cause grade 3-4 adverse events were hypertension (in 14 [3%] of 496 participants) and increased alanine aminotransferase (in 11 [2%]) in the pembrolizumab group, and hypertension (in 13 [3%] of 498 participants) in the placebo group. Serious adverse events attributed to study treatment occurred in 59 (12%) participants in the pembrolizumab group and one (<1%) participant in the placebo group. No deaths were attributed to pembrolizumab. INTERPRETATION: Updated results from KEYNOTE-564 support the use of adjuvant pembrolizumab monotherapy as a standard of care for participants with renal cell carcinoma with an increased risk of recurrence after nephrectomy. FUNDING: Merck Sharp & Dohme LLC, a subsidiary of Merck & Co, Inc, Rahway, NJ, USA.


Assuntos
Carcinoma de Células Renais , Hipertensão , Neoplasias Renais , Adulto , Anticorpos Monoclonais Humanizados , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma de Células Renais/tratamento farmacológico , Carcinoma de Células Renais/cirurgia , Método Duplo-Cego , Seguimentos , Humanos , Neoplasias Renais/tratamento farmacológico , Neoplasias Renais/etiologia , Neoplasias Renais/cirurgia , Nefrectomia/efeitos adversos
5.
Artigo em Inglês | MEDLINE | ID: mdl-36071361

RESUMO

Light and hydrodynamic force are important physical factors affecting growth of Microcystis. The most recent study found that green light has good effect in inhibiting growth of Microcystis. To understand the effect of mixing modes on Microcystis under the green light, we investigated the effects of continuous mixing and intermittent mixing on the abundance of Microcystis in Taihu Lake under field conditions. The study results found that abundance of Microcystis in control, intermittent mixing group, and continuous mixing group decreased 76.62%, 40.36%, and 95.18% on day 7 compared with that on day 1 in this experiment. At the end of the experiment, abundance percentages of diatoms and green algae to total phytoplankton abundance were 1.57% and 0.48% in control, 2.32% and 0.67% in intermittent mixing group, and 22.47% and 20.27% in continuous mixing group. The results indicated that continuous mixing favored the removal of Microcystis under green light conditions and was helpful for the growth of green algae and diatoms. The results provide a new approach for the control of Microcystis blooms.

6.
Rev Sci Instrum ; 93(8): 085004, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-36050122

RESUMO

A new multimodal bidirectional linear inertial impact motor with bidirectional motion based on self-clamping control driven by a single-harmonic signal was designed and manufactured. By applying driving signals of different resonant frequencies to the piezoelectric plate of a piezoelectric motor combined with the unique structural design of the motor, the piezoelectric motor has multiple modes and has the ability of two-way movement. First, the overall structure of the motor is introduced, and its working principle and theoretical displacement characteristics are presented through the periodic motion diagram of the piezoelectric motor. Second, the simulation analysis is carried out to determine the working modal of the proposed motor with COMSOL5.2. Finally, a motor prototype is developed, and the accuracy of the working principle and the simulation analysis is verified through experimental tests. When the motor has no load, the driving voltage is 200 Vp-p. The maximum speed when moving to the right reached 3.125 mm/s when the preload is 2 N, and the driving frequency is 96 Hz. The maximum speed when moving to the left reached 4.301 mm/s when the preload is 4 N, and the driving frequency is 148 Hz. In the load capacity test of the motor prototype, the maximum load of the piezoelectric motor prototype moving to the right and left can reach 0.4 and 0.6 N, respectively. Compared with similar inertial impact motors, the proposed motor achieves flexible control of driving and switching of two-way movement conveniently and has a certain driving ability.


Assuntos
Vibração , Simulação por Computador , Movimento (Física)
7.
ACS Omega ; 7(34): 30087-30092, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36061713

RESUMO

Carbon fiber-reinforced titanium matrix composites were prepared by powder metallurgy. Carbon fiber (CF) powder and titanium (Ti) powder are mixed, pressed, and then sintered at a high temperature of 1300-1500 °C. The morphology and conductivity of carbon fiber-reinforced titanium matrix (Ti-CF) composites were studied. When the temperature range of the Ti-CF composites was from 1300 to 1500 °C, the porosity and resistivity first decreased and then increased. When the sintering temperature was 1350 °C, the diffraction peak of the sample was the strongest, the porosity was the smallest (4.16%), and the resistivity was the smallest (2.7 MΩ·mm). CFs have a very good strengthening effect on titanium-based composite materials.

8.
Biomed Pharmacother ; 153: 113438, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36076553

RESUMO

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease and is an enormous burden on both patients and society. There is an urgent need for effective alternative therapeutic strategies for the treatment of DN, as medical treatment is currently limited. The anti-inflammatory, antioxidative, anti-apoptotic, and anti-fibrosis properties of curcumin, a polyphenol curcuminoid, have been demonstrated in research on diabetic nephropathy. The clinical and preclinical trials and mechanisms by which curcumin affects DN have been discussed in this review. A deeper understanding of the pharmacological effects of curcumin on diabetic nephropathy may provide new therapies to improve the development and occurrence of diabetic nephropathy.


Assuntos
Curcumina , Diabetes Mellitus , Nefropatias Diabéticas , Anti-Inflamatórios/farmacologia , Curcumina/farmacologia , Curcumina/uso terapêutico , Diabetes Mellitus/tratamento farmacológico , Nefropatias Diabéticas/tratamento farmacológico , Diarileptanoides , Humanos , Polifenóis/farmacologia , Polifenóis/uso terapêutico
9.
Commun Biol ; 5(1): 931, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36076060

RESUMO

The emergence and spread of the mcr-1 gene and its mutants has immensely compromised the efficient usage of colistin for the treatment of drug-resistant Gram-negative bacterial infection in clinical settings. However, there are currently no clinically available colistin synergis. Here we identify artemisinin derivatives, such as dihydroartemisinin (DHA), that produces a synergistic antibacterial effect with colistin against the majority of Gram-negative bacteria (FIC < 0.5) without induced resistance, particularly those carrying the mcr-1 gene. Mechanism analysis reveals the direct engagement of DHA with the active center of MCR-1 to inhibit the activity of MCR-1. Meanwhile, the results from transcriptome and electron microscope analysis show that DHA could also simultaneously affect the flagellar assembly and the energy metabolism of bacteria. Moreover, in the mouse infection models of Gram-negative bacteria, combination therapy shows remarkable treatment benefits, as shown by an improved survival rate, reduced morbidity, alleviated pathological injury and decreased bacterial loading. Due to the generally safe profile of specialized malaria medication administration in humans, artemisinin derivatives are a promising class of multi-target inhibitors on bacterial resistance and virulence that can be used to extend the usage life of colistin and to tackle the inevitability of serious bacterial infection with colistin.


Assuntos
Artemisininas , Infecções por Bactérias Gram-Negativas , Animais , Antibacterianos/farmacologia , Artemisininas/farmacologia , Colistina/farmacologia , Bactérias Gram-Negativas , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Humanos , Camundongos , Testes de Sensibilidade Microbiana
10.
J Hazard Mater ; 439: 129671, 2022 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-36104900

RESUMO

The extracellular electron transfer (EET) is regarded as one of the crucial factors that limit the application of the bioelectrochemical system (BES). In this study, two different solid-phase redox mediators (RMs), biochar (1.2 g/L, T-B) and humin (1.2 g/L, T-H) were used for boosting the microorganisms accessing the electrons required for 2,4,6-TCP dechlorination under weak electrical stimulation (-0.278 V vs. Standard hydrogen electrode). BES with dissolved RM anthraquinone-2,6-disulfonate (AQDS 0.5 mmol/L, T-A) was used as a comparison. The results showed that dechlorination of 2,4,6-TCP could be greatly accelerated by biochar (1.78 d-1) and humin (1.50 d-1) than AQDS (0.24 d-1) and no RM control (T-M, 0.27 d-1). Moreover, phenol became the predominant dechlorination product in T-H (78.5 %) and T-B (63.0 %) instead of 4-CP in T-M (67.1 %) and T-A (89.8 %). Pseudomonas, Sulfurospirillum, Desulfuromonas, Dehalobacter, Anaeromyxobacter, and Dechloromonas belonging to Proteobacteria or Firmicutes rather than Chloroflexi might be responsible for the dechlorination activity. Notably, different RMs tended to stimulate distinct electroactive bacteria. Pseudomonas was the most abundant microorganism in T-M (41.92 %) and T-A (17.24 %), while Rhodobacter was most prevalent in T-H (20.04 %) and Azonexus was predominant in T-B (48.48 %). This study is essential in advancing the understanding of EET in BES for microbial degradation of organohalide contaminants under weak electrical stimulation.


Assuntos
Halogenação , Ácidos Alcanossulfônicos , Biodegradação Ambiental , Carvão Vegetal , Clorofenóis , Estimulação Elétrica , Substâncias Húmicas
11.
Environ Res ; 215(Pt 1): 114222, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36049511

RESUMO

BACKGROUND: Alpine meadows, typical steppes, and deserts are among the globally important rangeland types that are generally distributed along temperature and precipitation gradients. Mineral losses caused by grazing are one of the key factors that can lead to instability or even degradation of these rangeland ecosystems. METHODS: We examined the concentrations of Cu, Fe, Mn, and Zn in soil, forage, and livestock dungs from diverse rangeland types in northwest China, to determine the relationships between these trace elements (TEs) concentrations and climatic factors (i.e., temperature, precipitation, and humidity), and to evaluate the potential risks of TEs deficiencies or excesses in these rangeland ecosystems. RESULTS: Forage Zn concentrations in forage of all three types of rangeland, and Cu concentrations in forage of the alpine meadow did not meet the growth requirements of grazing livestock. Concentrations of Cu, Fe, and Mn in forage and Fe, Mn, and Zn in livestock dungs had quadratic parabola relationships with temperature, precipitation, and humidity, but the relationships between climate factors and Cu, Fe, and Mn concentrations in soil were not significant. In addition, the abilities of the plant to absorb Cu, Fe, and Zn from soil were stronger in the typical steppe than that in the alpine meadows and desert. Also, the abilities of livestock to return TEs to soil were stronger in the alpine meadow than that in the typical steppe and desert. CONCLUSION: We derived a conceptual mode that the ratio of TE concentrations of the plant to soil and of livestock dung to forage represents the abilities of plants to absorb TEs from the soil matrix and livestock to return TEs to soil or to absorb TEs from forage, respectively. Results indicate potentially more serious risks of TEs deficiencies, especially that of Zn than previously considered in typical steppes and desert rangelands.

12.
ACS Appl Mater Interfaces ; 14(37): 41978-41987, 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36094174

RESUMO

Lithium lanthanum zirconium oxide (LLZO) has long been considered as a promising solid electrolyte for all-solid-state lithium (Li) metal batteries because of its interfacial stability when coupled with a Li metal anode. However, the cubic phase of LLZO (c-LLZO) with a higher Li-ion conductivity has a complex atomic structure and is subject to complicated phase transition during its processing and working conditions, which remain largely elusive. Here, we reveal the phase transition process during the formation of c-LLZO nanotubes through detailed microscopic characterization by scanning and transmission electron microscopy as well as X-ray diffraction. We find four typical stages during the formation of c-LLZO along with several intermediate phases including lanthanum (La)-rich cubic lanthanum zirconium oxide (La-rich c-LZO), c-LZO, and La-rich c-LLZO. We also reveal the role of m-Li2CO3 and h-Li2O2 as the "phase mediator".

13.
J Agric Food Chem ; 70(37): 11560-11571, 2022 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-36094400

RESUMO

In this study, 10 metabolites were obtained by collecting and extracting fecal samples after oral administration of panaxadiol (PD). Of these 10 metabolites, M7 (3ß,21ß,22α-hydroxy-24-norolean-12-ene), M8 (21ß,22α-hydroxy-24-norolean-12-ene-3-one), M9 (3ß,30α-hydroxy-24-norolean-22,30-epoxy-12-ene), and M10 (3ß,21ß-hydroxy-24-norolean-12-ene) were new compounds. MTT screening of the isolated compounds revealed that the inhibitions of cancer cells by M2, M4, M7, M8, and M10 were significantly stronger than that by the mother drug M0, with the activity of M2 being the most significant. Further, we investigated the anticancer mechanism of M2. The results showed that M2 significantly increased the level of ROS in cells; regulated the expressions of Bax, Bcl-2, and Cyt-C through the mitochondrial pathway; triggered the caspase cascade; and induced apoptosis. M2 could also induce G1 phase arrest and significantly regulate cell cycle-related proteins. In conclusion, the experimental results provide data for further study on the metabolic mechanism of PD in vivo and the potential of developing new anti-cancer drugs.

14.
Front Plant Sci ; 13: 964957, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36092398

RESUMO

Fertilizer management is vital for sustainable agriculture under climate change. Reduced basal and increased topdressing fertilizer rate (RBIT) has been reported to improve the yield of in-season rice or wheat. However, the effect of RBIT on rice and wheat yield stability and soil organic carbon (SOC) sequestration potential is unknown, especially when combined with straw incorporation. Here, we report the effect of RBIT with/without straw incorporation on crop yields, yield stability, SOC stock, and SOC fractions in the lower Yangtze River rice-wheat system region over nine years. RBIT with/without straw incorporation significantly increased nine-year average and annual rice yields but not wheat yields. Compared with conventional fertilization (CF), RBIT did not significantly affect wheat or rice yield stability, but combined with straw incorporation, it increased the sustainable yield index (SYI) of wheat and rice by 7.6 and 12.8%, respectively. RBIT produced a higher C sequestration rate (0.20 Mg C ha-1 year-1) than CF (0.06 Mg ha-1 year-1) in the 0-20 cm layer due to higher root C input and lower C mineralization rate, and RBIT in combination with straw incorporation produced the highest C sequestration rate (0.47 Mg ha-1 year-1). Long-term RBIT had a greater positive effect on silt+clay (0.053 mm)-associated C, microbial biomass C (MBC), dissolved organic C, and hot water organic C in the surface layer (0-10 cm) than in the subsurface layer (10-20 cm). In particular, the increases in SOC pools and mean weight diameter (MWD) of soil aggregates were greater when RBIT was combined with straw incorporation. Correlation analysis indicated that topsoil SOC fractions and MWD were positively correlated with the SYI of wheat and rice. Our findings suggest that the long-term application of RBIT combined with straw incorporation contributed to improving the sustainability of rice production and SOC sequestration in a rice-wheat system.

15.
Orthop Surg ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36125193

RESUMO

The patella's inferior pole transmits force generated by contraction of the quadriceps muscle to the tibial tuberosity through the attached patellar ligament, thus completing knee extension. Therefore, fractures of the patella's inferior pole disrupt the coherence of mechanical transmission in the lower extremities. There appears to be no consensus among trauma centers regarding the treatment of infrapatellar pole fractures, primarily because there is no consistent design or application of internal fixation for this type of fracture. We designed a new internal implant similar to the smile necklace based on our previous study. This smile-necklace plate (SNP) has the advantage of both plate fixation and tension-band wiring fixation, permitting early rehabilitation, especially in osteoporotic comminuted infrapatellar pole fractures. Finite element analysis helped verify the biomechanical advantages of the SNP in comparison with existing studies. Hence, this novel implant is a promising treatment option for inferior pole patellar fractures.

16.
Front Cell Infect Microbiol ; 12: 929856, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36046746

RESUMO

Objective: To evaluate the diagnostic performance of metagenomic next-generation sequencing (mNGS) and culture in pathogen detection among intensive care unit (ICU) and non-ICU patients with suspected pulmonary infection. Methods: In this prospective study, sputum samples were collected from patients with suspected pulmonary infection for 2 consecutive days and then subjected to DNA or RNA sequencing by mNGS or culture; 62 ICU patients and 60 non-ICU patients were admitted. In the end, comparisons were made on the pathogen species identified by mNGS and culture, the overall performance of these two methods in pathogen detection, and the most common pathogens detected by mNGS between the ICU and non-ICU groups. Results: In DNA and RNA sequencing, the positive rate of pathogen detection reached 96.69% (117/121) and 96.43% (108/112), respectively. In culture tests, the positive rate of the pathogen was 39.34% (48/122), much lower than that of DNA and RNA sequencing. In general, the positive rate of pathogen detection by sputum mNGS was significantly higher than that of sputum culture in the total and non-ICU groups (p < 0.001) but did not show a significant difference when compared to the result of sputum culture in the ICU group (p = 0.08). Haemophilus spp., Candida albicans, Enterococcus spp., and viruses from the mNGS results were excluded before comparing the overall performance of these two methods in pathogen detection. Specifically, among the 10 most common bacteria implied from the mNGS results, significant differences were observed in the number of cases of Haemophilus parainfluenzae, Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Staphylococcus aureus, and Enterococcus faecalis between the ICU and non-ICU groups (p < 0.05). Conclusions: This study demonstrated the superiority of mNGS over culture in detecting all kinds of pathogen species in sputum samples. These results indicate that mNGS may serve as a valuable tool to identify pathogens, especially for ICU patients who are more susceptible to mixed infections.


Assuntos
Metagenômica , Pneumonia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Metagenoma , Metagenômica/métodos , Pneumonia/microbiologia , Estudos Prospectivos , Sensibilidade e Especificidade
17.
Langmuir ; 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36099553

RESUMO

Defect engineering is a promising method for improving the performance of MoS2 in various fields. In this study, sulfur-defect-enriched MoS2 (SD-MoS2) nanosheets were fabricated via a facile microwave-hydrothermal strategy in 10 min for tetracycline (TC) adsorption applications. The introduction of sulfur defects in MoS2 induced more exposed unsaturated sulfur atoms at the edge, enhancing the interaction between the adsorbent and antibiotic and improving the adsorption activity of the antibiotic. Density functional theory calculations further revealed that sulfur defects in MoS2 could alter the electronic structure and exhibited low TC adsorption energy of -2.09 eV. This work provides a new method for fabricating MoS2 nanosheets and other transition metal dichalcogenide-based adsorbents with enhanced antibiotic removal performance and a comprehensive understanding of antibiotic removal mechanisms in SD-MoS2.

18.
Chem Biol Drug Des ; 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36102275

RESUMO

Given the difficult of experimental determination, quantitative structure-property relationship (QSPR) and deep learning (DL) provide an important tool to predict physicochemical property of chemical compounds. In this paper, partial least squares (PLS), genetic function approximation (GFA) and deep neural network (DNN) were used to predict the Lee retention index (Lee-RI) of PAHs in SE-52 and DB-5 stationary phases. Four molecular descriptors, molecular weight (MW), quantitative estimate of drug-likeness (QED), atomic charge weighted negative surface area (Jurs_PNSA_3) and relative negative charge (Jurs_RNCG) were selected to construct regression models based on genetic algorithm. For SE-52, PLS model showed best prediction power, followed by DNN and GFA. The relative error (RE), root mean square error (RMSE) and regression coefficient (R2 ) of best PLS regression model are 1.228%, 5.407 and 0.980. For DB-5, DNN model showed best prediction power, followed by GFA and PLS. The RE, RMSE and R2 of best DNN regression model for DB-5-1 and DB-5-2 are 1.058%, 4.325, 0.976, 0.821%, 3.795 0.970, respectively. The three regression models not only show good predictive ability, but also highlight the stability and ductility of the models.

19.
Ultrasonics ; 127: 106833, 2022 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-36070635

RESUMO

High-frame-rate plane wave (PW) imaging suffers from unsatisfactory image quality due to the absence of focus in transmission. Although coherent compounding from tens of PWs can improve PW image quality, it in turn results in a decreased frame rate, which is limited for tracking fast moving tissues. To overcome the trade-off between frame rate and image quality, we propose a progressively dual reconstruction network (PDRN) to achieve adaptive beamforming and enhance the image quality via both supervised and transfer learning in the condition of single or a few PWs transmission. Specifically, the proposed model contains a progressive network and a dual network to form a close loop and provide collaborative supervision for model optimization. The progressive network takes the channel delay of each spatial point as input and progressively learns coherent compounding beamformed data with increased numbers of steered PWs step by step. The dual network learns the downsampling process and reconstructs the beamformed data with decreased numbers of steered PWs, which reduces the space of the possible learning functions and improves the model's discriminative ability. In addition, the dual network is leveraged to perform transfer learning for the training data without sufficient steered PWs. The simulated, in vivo, vocal cords (VCs), and public available CUBDL dataset are collected for model evaluation.

20.
Nutrients ; 14(17)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36079746

RESUMO

The consumption of processed foods has increased compared to that of fresh foods in recent years, especially due to the coronavirus disease 2019 pandemic. Here, we evaluated the health effects of clarified apple juices (CAJs, devoid of pectin and additives) processed to different degrees, including not-from-concentrate (NFC) and from-concentrate (FC) CAJs. A 56-day experiment including a juice-switch after 28 days was designed. An integrated analysis of 16S rRNA sequencing and untargeted metabolomics of cecal content were performed. In addition, differences in the CAJs tested with respect to nutritional indices and composition of small-molecule compounds were analyzed. The NFC CAJ, which showed a higher phenolic content resulting from the lower processing degree, could improve microbiota diversity and influence its structure. It also reduced bile acid and bilirubin contents, as well as inhibited the microbial metabolism of tryptophan in the gut. However, we found that these effects diminished with time by performing experiment extension and undertaking juice-switching. Our study provides evidence regarding the health effects of processed foods that can potentially be applied to public health policy decision making. We believe that NFC juices with a lower processing degree could potentially be healthier than FC juice.


Assuntos
COVID-19 , Microbioma Gastrointestinal , Malus , Animais , Sucos de Frutas e Vegetais , Malus/química , Metabolômica/métodos , RNA Ribossômico 16S/genética , Ratos
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