Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.062
Filtrar
1.
PLoS One ; 15(10): e0240143, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33007026

RESUMO

Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V. parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-field gel electrophoresis (PFGE) for molecular typing. O3:K6 was the main serotype and tlh+tdh+trh- was the most frequently detected virulence genotype in clinical strains. O2:Kut was the main serotype and tlh+tdh-trh- was the most frequently detected virulence genotype in environmental strains. Antibiotic resistance testing indicated that the isolates were highly resistant to ampicillin (90.76%), followed by gentamicin and tetracycline. Following the restriction enzyme NotI digestion, the 91 strains yielded 81 PFGE patterns, and 16 clones had similarity values of > 85.00%, indicating a high level of diversity. Finally, there may be cross-contamination between freshwater and seawater products, so it is necessary to strengthen supervision of food processing.

2.
Biol Reprod ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33030202
3.
Cardiovasc Diabetol ; 19(1): 161, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32998742

RESUMO

BACKGROUND: Type 2 diabetes mellitus (T2DM) increases the risks of heart failure and mortality in patients with hypertension, however the underlying mechanism is unclear. This study aims to investigate the impact of coexisting T2DM on left ventricular (LV) deformation and myocardial perfusion in hypertensive individuals. MATERIALS AND METHODS: Seventy hypertensive patients without T2DM [HTN(T2DM-)], forty patients with T2DM [HTN(T2DM+)] and 37 age- and sex-matched controls underwent cardiac magnetic resonance examination. Left ventricular (LV) myocardial strains, including global radial (GRPS), circumferential (GCPS) and longitudinal peak strain (GLPS), and resting myocardial perfusion indices, including upslope, time to maximum signal intensity (TTM), and max signal intensity (MaxSI), were measured and compared among groups by analysis of covariance after adjusting for age, sex, body mass index (BMI) and heart rate followed by Bonferroni's post hoc test. Backwards stepwise multivariable linear regression analyses were performed to determine the effects of T2DM on LV strains and myocardial perfusion indices in patients with hypertension. RESULTS: Both GRPS and GLPS deteriorated significantly from controls, through HTN(T2DM-), to HTN(T2DM+) group; GCPS in HTN(T2DM+) group was lower than those in both HTN(T2DM-) and control groups. Compared with controls, HTN(T2DM-) group showed higher myocardial perfusion, and HTN(T2DM+) group exhibited lower perfusion than HTN(T2DM-) group and controls. Multiple regression analyses considering covariates of systolic blood pressure, age, sex, BMI, heart rate, smoking, indexed LV mass and eGFR demonstrated that T2DM was independently associated with LV strains (GRPS: p = 0.002, model R2= 0.383; GCPS: p < 0.001, model R2= 0.472; and GLPS: p = 0.002, model R2= 0.424, respectively) and perfusion indices (upslope: p < 0.001, model R2= 0.293; TTM: p < 0.001, model R2= 0.299; and MaxSI: p < 0.001, model R2= 0.268, respectively) in hypertension. When both T2DM and perfusion indices were included in the regression analyses, both T2DM and TTM were independently associated with GRPS (p = 0.044 and 0.017, model R2= 0.390) and GCPS (p = 0.002 and 0.001, model R2= 0.424), and T2DM but not perfusion indices was independently associated with GLPS (p = 0.002, model R2= 0.424). CONCLUSION: In patients with hypertension, T2DM had an additive deleterious effect on subclinical LV systolic dysfunction and myocardial perfusion, and impaired myocardial perfusion by coexisting T2DM was associated with deteriorated LV systolic dysfunction.

4.
Comp Biochem Physiol C Toxicol Pharmacol ; 239: 108894, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32949816

RESUMO

Beta-cypermethrin, a type II synthetic pyrethroid insecticide, is widely used in pest control. Several studies have demonstrated that beta-cypermethrin can affect the reproductive system of mammals. However, there is still a scarcity of information about the reproductive toxicity to fish induced by beta-cypermethrin and its molecular mechanism. Therefore, this study was conducted to address this scientific question, in which the adult zebrafish were exposed to 0 (blank control), 0 (acetone solvent control), 0.1, 0.5, and 2.5 µg/L of beta-cypermethrin for 21 days. A decrease in cumulative egg production of zebrafish was observed, indicating that beta-cypermethrin had a negative impact on reproductive capacity of zebrafish. Regarding the histomorphological analysis of gonads, the delay of gonadal development was observed after exposure for 21 days. In addition, significant changes in plasma 17ß-estradiol (E2) and testosterone (T) were found in zebrafish. Further exploration showed that the transcription levels of hypothalamic-pituitary-gonadal (HPG) axis-related genes were remarkably changed, which corresponded well with the alterations of hormone levels. These results demonstrated that beta-cypermethrin might have an adverse effect on the reproduction system of zebrafish through delaying gonadal development, disturbing sex hormone secretion, and affecting HPG axis gene expression. This study suggests that beta-cypermethrin poses a potential threat to the reproduction of fish populations, and the toxicity assessment of beta-cypermethrin plays a vital role in the environmental risk assessment of pesticides.

5.
Oncol Rep ; 44(4): 1638-1648, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32945522

RESUMO

Lung carcinoma is a prominent cause of mortality among patients with cancer. Previous studies have reported the vital role of long non­coding RNAs (lncRNAs) in the malignant progression of lung cancer. lncRNA RP11­284F21.9 was originally identified to be expressed in lung carcinoma, but its specific function remains unknown. Therefore, the present study aimed to elucidate the role of lncRNA RP11­284F21.9 in lung carcinoma progression. The expression of RP11­284F21.9 in lung cell lines and tissues was measured using reverse transcription­quantitative PCR. The endogenous expression of RP11­284F21.9 was silenced using RNA interference, and cell viabilities were measured with a Cell Counting Kit­8 assay. The invasion and apoptosis of cells were determined via Transwell assays and flow cytometry, respectively. The protein expression levels were measured by western blotting. An increased expression of RP11­284F21.9 was identified in both lung carcinoma tissues and cells. Knockdown of RP11­284F21.9 in lung carcinoma cells inhibited cell proliferation and invasion, but promoted cell apoptosis. The present study identified the existence of a direct interaction between RP11­284F21.9 and microRNA (miRNA/miR)­627­3p. Mechanistically, it was demonstrated that RP11­284F21.9 promoted the proliferation and invasiveness of lung carcinoma cells, in part, via the regulation of miR­627­3p. Furthermore, cell division cycle and apoptosis regulator 1 (CCAR1) was identified as a target gene of miR­627­3p. The in vivo tumor growth assay also demonstrated that the knockdown of RP11­284F21.9 suppressed tumor growth, upregulated miR­627­3p and downregulated CCAR1 in the xenograft model of nude mice. Thus, the present findings indicated the tumor promoting functions of RP11­284F21.9 in the progression of lung carcinoma, and provided a novel lncRNA/miRNA axis as a target for the management of lung cancer.

6.
Environ Res ; 189: 109926, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32980014

RESUMO

While accumulating evidence shows that air pollution exposure is an important risk factor to influenza prevalence, their association has been inadequately investigated in mountainous regions with dense populations and high humidity. We aim to estimate the association and exposure-outcome effects between exposure to nitrogen dioxide (NO2) and influenza prevalence in a mountainous region with a dense population and high humidity. We investigated 14,993 patients with confirmed influenza cases from January 2013 to December 2017 in Chongqing, a mountainous city in southwest China. We developed distributed lag non-linear models with quasi-Poisson link to take into account the lag and non-linear effects of NO2 exposure on influenza prevalence. We estimated that the cumulative effect of a 10 µg/m3 increase in NO2 with seven-day lag (i.e., summing all the contributions up to seven days) corresponded to relative risk of 1.24 (95% CI: 1.17-1.31) in daily influenza prevalence. Comparing to annual mean of the World Health Organization air quality guidelines of 40 µg/m3 for NO2, we estimated that 14.01% (95% CI: 10.69-17.08%) of the influenza cases were attributable to excessive NO2 exposure. Our results suggest that NO2 exposure could worsen the risk of influenza infection in this mountainous city, filling the gap of relevant researches in densely populated and mountainous cities. Our findings provide evidence for developing influenza surveillance and early warning systems.

7.
Atherosclerosis ; 312: 43-53, 2020 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-32971395

RESUMO

BACKGROUND AND AIMS: Long noncoding RNAs (lncRNAs) have recently been implicated in many biological and disease processes, but the exact mechanism of their involvement in atherosclerosis is unclear. The aberrant proliferation and migration of vascular smooth muscle cells (VSMCs) is a major contributor to the development of atherosclerotic lesions. This study aimed to investigate the potential effects of lncRNA ZNF800, a previously uncharacterized lncRNA, on VSMC proliferation and migration. METHODS: The expression of lncRNA ZNF800 in atherosclerotic plaque tissues was detected using reverse transcription-quantitative PCR (RT-qPCR), while the role and mechanism of lncRNA ZNF800 in proliferation and migration of VSMCs were investigated by CCK8 assay, transwell assay, scratch wound assay, RT-qPCR and Western blot. RESULTS: We found that lncRNA ZNF800 was significantly more abundant in atherosclerotic plaque tissues, and substantially suppressed the proliferation and migration of VSMCs. LncRNA ZNF800 had no effect on phosphatase and tensin homolog deleted on chromosome 10 (PTEN) mRNA expression but dramatically increased the levels of PTEN protein. Enhanced lncRNA ZNF800 expression inhibited the activity of the AKT/mTOR/HIF-1α signaling pathway, downregulated the expression of vascular endothelial growth factor α (VEGF-α) and matrix metalloproteinase 1 (MMP1), and suppressed VSMC proliferation and migration. These inhibitory effects of lncRNA ZNF800 were abolished by knockdown of PTEN. The inhibitory effects of lncRNA ZNF800 on cell proliferation and migration and the expression of VEGF-α and MMP1 were exacerbated by HIF-1α knockdown in VSMCs. CONCLUSIONS: These findings demonstrated that lncRNA ZNF800 suppressed VSMC proliferation and migration by interacting with PTEN through a mechanism involving AKT/mTOR/HIF-1α signaling. Therefore, it may play a key atheroprotective role and represent a potential therapeutic target for atherosclerosis-related diseases.

8.
Biomed Environ Sci ; 33(8): 557-565, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32933607

RESUMO

Objective: To determine the prevalence and determinants of folic acid (FA) supplementation in Chinese couples planning for pregnancy and in women during early pregnancy. Methods: This was a cross-sectional study based on the Shanghai PreConception Cohort (SPCC) study. Data on FA supplementation and socio-demographic features were collected using questionnaires. Couples visiting clinics for pre-pregnancy examination and pregnant women at < 14 gestational weeks were recruited in Shanghai, China, between March 2016 and September 2018. Results: Among the pregnancy planners, 42.4% (4,710/11,099) women and 17.1% (1,377/8,045) men used FA supplements, while 93.4% (14,585/15,615) of the pregnant women used FA supplements. FA supplement use was higher in female pregnancy planners who were older ( RR: 1.13, 95% CI: 1.08-1.18), had higher education ( RR: 1.71, 95% CI: 1.53-1.92), and were residing in urban districts ( RR: 1.06, 95% CI: 1.01-1.11) of FA supplementation; female pregnancy planners with alcohol consumption ( RR: 0.95, 95% CI: 0.90-0.99) had lower odds of FA supplementation. In early pregnancy, women with higher educational level ( RR: 1.04, 95% CI: 1.03-1.06), who underwent pre-pregnancy examination ( RR: 1.02, 95% CI: 1.01-1.03) had higher odds of using an FA supplement; older aged ( RR: 0.99, 95% CI: 0.98-0.99), and multigravida ( RR: 0.97, 95% CI: 0.96-0.98) had lower odds of FA supplementation. Conclusion: Although the majority of pregnant women took FA supplements, more than half of the women planning for pregnancy did not. Urgent strategies are needed to improve pre-conception FA supplementation.


Assuntos
Suplementos Nutricionais/análise , Ácido Fólico/administração & dosagem , Complexo Vitamínico B/administração & dosagem , Adulto , China , Estudos de Coortes , Estudos Transversais , Dieta , Feminino , Humanos , Masculino , Gravidez , Inquéritos e Questionários , Adulto Jovem
9.
Am J Sports Med ; 48(12): 2954-2961, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32866043

RESUMO

BACKGROUND: Steep posterior tibial slope (PTS) and excessive anterior tibial translation (ATT) have been identified as important anatomic risk factors for anterior cruciate ligament (ACL) injury, which have raised concerns about clinical outcomes after primary ACL reconstruction (ACLR). PURPOSE: To investigate anatomic risk factors of primary ACLR failure and to determine the cutoff values of PTS and ATT for predicting primary ACLR failure. STUDY DESIGN: Case-control study; Level of evidence, 3. METHODS: Between November 2015 and May 2017, a total of 215 consecutive patients with clinically diagnosed noncontact ACL injuries who underwent primary anatomic ACLR were retrospectively analyzed. Among them, 25 patients who showed complete discontinuity of ACL fibers on final follow-up magnetic resonance imaging scans were allocated into the failure group (study group). They were matched 1:2 to 50 control participants who showed clear and continuous ACL fibers on magnetic resonance imaging scans (control group). PTS and ATT were measured on preoperative weightbearing whole leg lateral radiographs and compared between the groups. The cutoff values of PTS and ATT for predicting primary ACLR failure were determined by the receiver operating characteristic curve. Moreover, predictors of primary ACLR failure were assessed by multivariate logistic regression analysis, including sex, age, body mass index, concomitant meniscal tears, degree of pivot-shift test, and KT-1000 arthrometer side-to-side difference, PTS, and ATT. RESULTS: PTS and ATT values in the study group were significantly higher than those in the control group (mean ± SD: PTS, 17.2°± 2.2° vs 14.4°± 2.8°; ATT, 8.3 ± 3.4 mm vs 4.1 ± 3.1 mm; P < .001). The cutoff values of PTS and ATT for predicting primary ACLR failure were 17° (sensitivity, 66.7%; specificity, 90.9%) and 6 mm (sensitivity, 87.5%; specificity, 79.5%), respectively. Additionally, PTS ≥17° (odds ratio, 15.6; 95% CI, 2.7-91.5; P = .002) and ATT ≥6 mm (odds ratio, 9.9; 95% CI, 1.9-51.4; P = .006) were determined to be risk factors of primary ACLR failure, whereas sex, age, body mass index, concomitant meniscal tears, degree of the pivot-shift test, and KT-1000 arthrometer side-to-side difference were not. CONCLUSION: In this study, PTS ≥17° and ATT ≥6 mm, as measured on weightbearing whole leg radiographs, were identified to be predictive risk factors of primary ACLR failure. This study adds to the existing knowledge about potential surgical indications of simultaneous slope-reducing high tibial osteotomy to mitigate the primary ACLR failure rate.

10.
Hepatology ; 2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-32865250

RESUMO

We read with great interest the study by Ishikawa et al. The authors found that induction of the mouse major histocompatibility complex (MHC) antigen, blastocyst H2 (H2-Bl) expression by growth hormone is critical for suppressing innate immune cells such as natural killer (NK) cells/NK T cells (NKTs) and macrophage-mediated hepatocyte apoptosis, which favors C57BL/6 mice liver regeneration and survival after partial hepatectomy.

11.
J Nat Prod ; 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32975117

RESUMO

The mangrove-derived endophytic fungus Peniophora incarnata Z4 produced seven new xanthone derivatives, including four new tetrahydroxanthones (1-4), one new chromone (5), one new xanthone (6), and one new xanthone dimer (7), together with one known compound, globosuxanthone B (8). Their structures were determined by an extensive analysis of 1D and 2D NMR, HRESIMS, ECD, and single-crystal X-ray diffraction data. In cytotoxic activity assays, compound 2 showed cytotoxicity against three carcinoma cell lines with IC50 values less than 10 µM.

12.
Cell Stem Cell ; 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32966778

RESUMO

Although cell transplantation can rescue motor defects in Parkinson's disease (PD) models, whether and how grafts functionally repair damaged neural circuitry in the adult brain is not known. We transplanted hESC-derived midbrain dopamine (mDA) or cortical glutamate neurons into the substantia nigra or striatum of a mouse PD model and found extensive graft integration with host circuitry. Axonal pathfinding toward the dorsal striatum was determined by the identity of the grafted neurons, and anatomical presynaptic inputs were largely dependent on graft location, whereas inhibitory versus excitatory input was dictated by the identity of grafted neurons. hESC-derived mDA neurons display A9 characteristics and restore functionality of the reconstructed nigrostriatal circuit to mediate improvements in motor function. These results indicate similarity in cell-type-specific pre- and post-synaptic integration between transplant-reconstructed circuit and endogenous neural networks, highlighting the capacity of hPSC-derived neuron subtypes for specific circuit repair and functional restoration in the adult brain.

13.
Enzyme Microb Technol ; 140: 109629, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32912689

RESUMO

2-Phenylethanol (2-PE) production through bio-synthesis method has become an appealing option owning to the mild conditions and high product selectivity. However, 2-PE is toxic to cells, which is an important limiting factor for the biosynthesis of 2-PE. In this study, a novel 2-PE generating Meyerozyma sp. strain YLG18 was first isolated, which could produce 2-PE through both Ehrlich and Shikimate pathways. Moreover, the indigenous high 2-PE tolerance makes it a promising candidate for high 2-PE production. Response surface methodology and in situ product recovery technology could improve the final 2-PE production to 3.20 g/L, representing the highest 2-PE production by using Meyerozyma sp. Furthermore, genes involved in 2-PE synthesis were identified and their expression levels between Shikimate pathway and Ehrlich pathway were compared. Based on the genomic and transcriptional analysis, a penta-functional enzyme AroM and an aspartate aminotransferase (AAT) with the potential to convert phenylalanine into phenylpyruvate were identified. These findings would help broaden our knowledge and add the pool of known 2-PE generating microbes and genes.

14.
Zhongguo Zhong Yao Za Zhi ; 45(16): 3900-3907, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893587

RESUMO

A highly sensitive monoclonal antibody against aflatoxin B_1(AFB_1) was prepared and an indirect competition enzyme-linked immunosorbent assay(ic-ELISA) was established based on the antibody which was used for high-throughput and rapid screening of AFB_1 contamination in Chinese herbal medicines to ensure the safety of medication. In this study, the structure of AFB_1 was modified by improved oxime method, and the carrier protein was coupled by EDC-NHS method to obtain the complete antigen of AFB_1, which was more convenient and environmental friendly. The Balb/c female mice were immunized using increasing the immunization dose and various ways of injection, and finally the AFB_1 monoclonal antibody was prepared. The AFB_1 monoclonal antibody belongs to IgG_(2 b) immunoglobulin by identifying its immunological characteristics, and its sensitivity(IC_(50)) can reach 0.15 µg·L~(-1), and the affi-nity is 2.81×10~8 L·mol~(-1). The cross-reaction rates of AFB_2, AFG_1, and AFG_2 were 35.07%, 8.75%, and 1.15%, respectively, and there was almost no cross-reactivity with other mycotoxins. Based on the high sensitivity and specificity of the antibody, an ic-ELISA method was established and applied to the determination of AFB_1 contamination in Ziziphi Spinosae Semen. According to the matrix matching standard curve, the linear concentration range for AFB_1 was 0.05-0.58 µg·L~(-1)(R~2=0.992), the recoveries were 88.00%-119.0%, and the detection limit was 1.69 µg·kg~(-1). The AFB_1 in 33 batches of Ziziphi Spinosae Semen samples was determined by ic-ELISA, and the contamination level was 3.62-206.58 µg·kg~(-1). The linear correlation coefficient between the detection results of ic-ELISA and UHPLC-MS/MS was 0.996, and there were no false positive and false negative cases. It indicates that the established ic-ELISA is accurate and reliable, and could provide a simple and effective technique for fast screening of AFB_1 contamination in Ziziphi Spinosae Semen, and also could be considered as the reference for the detection and monitoring of AFB_1 contamination in other Chinese herbal medicines.


Assuntos
Aflatoxina B1/análise , Sêmen/química , Animais , Anticorpos Monoclonais , Contaminação de Medicamentos , Ensaio de Imunoadsorção Enzimática , Feminino , Camundongos , Espectrometria de Massas em Tandem
15.
Biomark Med ; 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32927965

RESUMO

Aims: To explore new SNP sites of miRNAs associated with gastric cancer, thereby providing valuable biomarkers to diagnose and screen gastric cancer. Materials & methods: A 1:1 case-control study was carried out. Microarrays were used to screen the SNP loci of miRNAs in the genomes of matched pairs of patients, 96 with gastric cancer and 96 healthy controls. For validation, mass spectrometry was used to classify miRNA SNP loci in 622 pairs of subjects. Results: rs7143252 was linked to a higher occurrence of gastric cancer. Conclusions: These results suggest that rs7143252 could be used as a specific biomarker to diagnose and screen gastric cancer.

16.
J Med Syst ; 44(10): 182, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32885290

RESUMO

Physiological signals can contain abundant personalized information and indicate health status and disease deterioration. However, in current medical practice, clinicians working in the general wards are usually lack of plentiful means and tools to continuously monitor the physiological signals of the inpatients. To address this problem, we here presented a medical-grade wireless monitoring system based on wearable and artificial intelligence technology. The system consists of a multi-sensor wearable device, database servers and user interfaces. It can monitor physiological signals such as electrocardiography and respiration and transmit data wirelessly. We highly integrated the system with the existing hospital information system and explored a set of processes of physiological signal acquisition, storage, analysis, and combination with electronic health records. Multi-scale information extracted from physiological signals and related to the deterioration or abnormality of patients could be shown on the user interfaces, while a variety of reports could be provided daily based on time-series signal processing technology and machine learning to make more information accessible to clinicians. Apart from an initial attempt to implement the system in a realistic clinical environment, we also conducted a preliminary validation of the core processes in the workflow. The heart rate veracity validation of 22 patient volunteers showed that the system had a great consistency with ECG Holter, and bias for heart rate was 0.04 (95% confidence interval: -7.34 to 7.42) beats per minute. The Bland-Altman analysis showed that 98.52% of the points were located between Mean ± 1.96SD. This system has been deployed in the general wards of the Hyperbaric Oxygen Department and Respiratory Medicine Department and has collected more than 1000 cases from the clinic. The whole system will continue to be updated based on clinical feedback. It has been demonstrated that this system can provide reliable physiological monitoring for patients in general wards and has the potential to generate more personalized pathophysiological information related to disease diagnosis and treatment from the continuously monitored physiological data.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32882403

RESUMO

BACKGROUND & AIMS: Pancreatic ductal adenocarcinoma (PDA) initiation and progression are accompanied by an immunosuppressive inflammatory response. Here, we evaluated the immunomodulatory role of chemosensory signaling in metaplastic tuft cells (MTCs) by analyzing the role of GNAT3, a gustatory pathway G-protein expressed by MTCs, during PDA progression. METHODS: Gnat3-null (Gnat3-/-) mice were crossbred with animals harboring a Cre-inducible KrasLSL-G12D/+ allele with either Ptf1aCre/+ (KC) or tamoxifen-inducible Ptf1aCreERT/+ (KCERT) mice to drive oncogenic KRAS expression in the pancreas. Ex vivo organoid conditioned medium generated from KC and Gnat3-/-;KC acinar cells was analyzed for cytokine secretion. Experimental pancreatitis was induced in KCERT and Gnat3-/-;KCERT mice to accelerate tumorigenesis, followed by analysis using mass cytometry and single-cell RNA sequencing. To study PDA progression, KC and Gnat3-/-;KC mice were aged to morbidity or 52 weeks. RESULTS: Ablation of Gnat3 in KC organoids increased release of tumor-promoting cytokines in conditioned media, including CXCL1 and CXCL2. Analysis of Gnat3-/-;KCERT pancreata found altered expression of immunomodulatory genes in Cxcr2 expressing myeloid-derived suppressor cells (MDSCs) and an increased number of granulocytic MDSCs, a subset of tumor promoting MDSCs. Importantly, expression levels of CXCL1 and CXCL2, known ligands for CXCR2, were also elevated in Gnat3-/-;KCERT pancreata. Consistent with the tumor-promoting role of MDSCs, aged Gnat3-/-;KC mice progressed more rapidly to metastatic carcinoma compared with KC controls. CONCLUSIONS: Compromised gustatory sensing, achieved by Gnat3 ablation, enhanced the CXCL1/2-CXCR2 axis to alter the MDSC population and promoted the progression of metastatic PDA.

18.
Artigo em Inglês | MEDLINE | ID: mdl-32924190

RESUMO

Cryptocaryon irritans, a pathogen model for fish mucosal immunity, causes skin mucosal and systematic humoral immune response. Where and how MHC II antigen presentation occurs in fish infected with C. irritans remain unknown. In this study, the full-length cDNA of the grouper cysteine protease CTSS was cloned. The expression distributions of six genes (CTSB, CTSL, CTSS, GILT, MHC IIA and MHC IIB) involved in MHC II antigen presentation pathway were tested. These genes were highly expressed in systematic immune tissues and skin and gill mucosal-associated immune tissues. All six genes were upregulated in skin at most time points. Five genes expected CTSS was upregulated in spleen at most time points. CTSB, CTSL and MHC IIA were upregulated in the gill and head kidney at some time points. These results indicate that the presentation of MHC II antigen intensively occurred in local infected skin and gill. Spleen, not head kidney, had the most extensive systematic antigen presentation. In skin, six genes most likely peaked at day 2, earlier than in spleen (5-7 days), marking an earlier skin antibody peak than any recorded in serum previously. This significant and earlier mucosal antigen presentation indicates that specific immune response occurs in local mucosal tissues.

19.
ACS Nano ; 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32955859

RESUMO

Thin two-dimensional (2D) material absorbers have the potential to reduce volume-dependent thermal noise in infrared detectors. However, any reduction in noise must be balanced against lower absorption from the thin layer, which necessitates advanced optical architectures. Such architectures can be particularly effective for applications that require detection only within a specific narrow wavelength range. This study presents a Fabry-Pérot cavity enhanced bP/MoS2 midwave infrared (MWIR) photodiode. This simple structure enables tunable narrow-band (down to 0.42 µm full width at half-maximum) photodetection in the 2-4 µm range by adjusting the thickness of the Fabry-Pérot cavity resonator. This is achieved while maintaining room-temperature performance metrics comparable to previously reported 2D MWIR detectors. Zero bias specific detectivity and responsivity values of up to 1.7 × 109 cm Hz1/2 W-1 and 0.11 A W-1 at λ = 3.0 µm are measured with a response time of less than 3 ns. These results introduce a promising family of 2D detectors with applications in MWIR spectroscopy.

20.
Colloids Surf B Biointerfaces ; 196: 111304, 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32777663

RESUMO

Oxidative stress induced by reactive oxygen species (ROS) overproduction and accumulation would hinder the osseointegration process at the bone-implant interface, leading to a higher rate of implant failure. To endow titanium (Ti) implants with antioxidant activity, we developed a coating approach mediated by tannic acid (TA)-Ca2+ coordination complexation. A hydroxyapatite (HA)/TA composite coating was prepared, based on Ti substrates modified by anodized and annealed titanium dioxide (TiO2) nanotube arrays. The results reveal that highly ordered TiO2 nanotubes with a diameter of 142.23 ±â€¯14.52 nm and a length of 374.17 ±â€¯42.47 nm were fabricated on the Ti substrate and the XRD pattern shows the TiO2 anatase phase after annealing at 450 ℃. TA-Ca complexes were formed on the surface of TiO2 nanotubes by immersing the constructs into the mixed solution of TA and CaCl2, where they are served as calcium sites for the HA growth by later phosphorylation. The HA nanoparticles present needle-shape with the diameter of 18 ∼ 20 nm. The total antioxidant capacity assay was employed to confirm the antioxidant effect of the HA/TA composite coating. The results indicate that it has a persistent and strong antioxidative activity. In vitro cytological test results show that HA/TA coating exhibits good cytocompatibility for osteoblasts proliferation and adhesion.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA