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1.
Eur J Pharmacol ; 889: 173614, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33010304

RESUMO

The present study aimed to assess the effect of sigma-1 receptor (S1R) stimulation on ventricular remodeling and susceptibility to ventricular arrhythmias (VAs) after myocardial infarction (MI) in rats. Wild-type male rats were placed into one of the following four treatment groups. For four weeks, animals in the Sham group and MI group received intraperitoneal (i.p.) injections of 0.9% saline (1 ml/kg/day); those in the MI + F group received fluvoxamine (FLV) (0.3 mg/kg/day); and those in the MI + F + BD group received FLV plus BD1047 (0.3 mg/kg/day). After that, the ventricular electrophysiological parameters were measured via the langendorff system. Ventricular fibrosis quantification was determined with Masson staining. Cardiac function was evaluated by echocardiography. The protein levels of S1R, connexin (Cx)43, Cav1.2, Kv4.2, Kv4.3, tyrosine hydroxylase (TH), nerve growth factor (NGF), growth-associated protein 43 (GAP43) were detected by Western blot assays. Our results indicated that fluvoxamine significantly prolonged the ventricular effective refractory period (ERP), shortened action potential duration (APD), reduced susceptibility to VAs after MI. Masson staining showed a decrease in ventricular fibrosis in the MI + F group. Furthermore, the contents of Cx43, S1R, Cav1.2, Kv4.2, Kv4.3 were increased in the MI + F group compared with the MI group (all P < 0.05). The contents of TH, NGF, GAP43 were reduced in the MI + F group compared with the MI group. (all P < 0.05). However, BD1047 reduces all of these effects of FLV. The results suggest that S1R stimulation reduces susceptibility to VAs and improves cardiac function by improving myocardial fibrosis, lightning sympathetic remodeling, electrical remodeling, gap junction remodeling and upregulating S1R content.

2.
Environ Res ; 192: 110289, 2020 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-33027626

RESUMO

BACKGROUND: Previous studies have indicated that exposure to green space may benefit human health. However, the available evidence concerning the effects of greenness, especially school-based greenness, on pediatric obesity is scarce. OBJECTIVE: To explore the association between school-based greenness and adiposity in children and adolescents in China. METHOD: We conducted a nationwide cross-sectional study of 56,620 children and adolescents (aged 6-18 years) in seven provinces/municipalities across China. School-based greenness was assessed using satellite-derived Normalized Difference Vegetation Index (NDVI) and Soil Adjusted Vegetation Index (SAVI) within 100-, 500-, and 1000-m circular buffers around each school's address. Generalized linear mixed regression models were used to estimate associations of greenness with BMI z-scores (zBMI), waist circumference, and prevalent overweight/obesity. We also explored the potential mediating role of ambient air pollution and physical activity in the greenness-adiposity associations. RESULT: In the adjusted model, an IQR increase in NDVI-1000m was associated with lower zBMI (ß: -0.11, 95% confidence interval[CI]: -0.13,-0.09) and waist circumference (ß: -0.64, 95%CI: -0.78,-0.50). Consistently, an IQR increase in NDVI-100m, NDVI-500m, NDVI-1000m was associated with 7-20% lower odds of overweight/obesity in the adjusted models. Air pollutants mediated 6.5-29.1% of the association between greenness and zBMI. No significant mediation effect was observed for physical activity. CONCLUSION: Higher school-based greenness levels were associated with lower zBMI, waist circumference, and lower odds of overweight and obesity in children and adolescents. Ambient air pollutants may partially mediate the greenness-adiposity associations.

3.
Artigo em Inglês | MEDLINE | ID: mdl-33048764

RESUMO

We propose a general and scalable global optimization framework directly operating on annotated graph data by introducing a Bayesian graph neural network to approximate the expensive-to-evaluate objectives. It prevents the cubical complexity of Gaussian processes and can scale linearly with the number of observations. Its parallelized variant makes it scalable. We provide strict theoretical support on its convergence. Intensive experiments conducted on both artificial and real-world problems, including molecular discovery and urban road network design, demonstrate the effectiveness of the proposed methods compared with the current state of the art.

4.
J Clin Lab Anal ; : e23619, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33067888

RESUMO

BACKGROUND: The ongoing coronavirus disease 19 (COVID-19) is posing a threat to the public health globally. Serological test for SARS-CoV-2 antibody can improve early diagnosis of COVID-19 and serves as a valuable supplement to RNA detection. METHOD: A SARS-CoV-2 IgG/IgM combined antibody test strip based on colloidal gold immunochromatography assay was developed, with both spike protein and nucleocapsid protein of SARS-CoV-2 antigen used for antibody detection. From 3 medical institutions across China, serum or plasma of 170 patients with confirmed COVID-19 diagnosis and 300 normal controls were collected and tested with the strip. Sensitivity, specificity, kappa coefficient, receiver operating characteristic (ROC) curve, and area under the curve (AUC) were analyzed. Positive rates in different medical centers, age group, gender, and different disease course were compared. RESULTS: 158 out 170 samples from confirmed COVID-19 patients had positive results from the test, and 296 out of 300 samples from normal controls had negative results. The kit was 92.9% sensitive and 98.7% specific. The positive rate was 77.3% during the first week after disease onset, but reached 100% since day 9. AUC and kappa coefficient were 0.958 and 0.926, respectively, which showed the consistency of the test results with the standard diagnosis. Age or gender caused little variations in the kit sensitivity. CONCLUSION: The rapid, easy-to-use SARS-CoV-2 IgG/IgM combined antibody test kit has a superior performance, which can help with accurate diagnosis and thus timely treatment and isolation of COVID-19 patients, that contributes to the better control of the global pandemic.

5.
Neurosci Bull ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33048310

RESUMO

General anesthesia severely affects the metabolites in the brain. Glycogen, principally stored in astrocytes and providing the short-term delivery of substrates to neurons, has been implicated as an affected molecule. However, whether glycogen plays a pivotal role in modulating anesthesia-arousal remains unclear. Here, we demonstrated that isoflurane-anesthetized mice exhibited dynamic changes in the glycogen levels in various brain regions. Glycogen synthase (GS) and glycogen phosphorylase (GP), key enzymes of glycogen metabolism, showed increased activity after isoflurane exposure. Upon blocking glycogenolysis with 1,4-dideoxy-1,4-imino-D-arabinitol (DAB), a GP antagonist, we found a prolonged time of emergence from anesthesia and an enhanced δ frequency in the EEG (electroencephalogram). In addition, augmented expression of glycogenolysis genes in glycogen phosphorylase, brain (Pygb) knock-in (PygbH11/H11) mice resulted in delayed induction of anesthesia, a shortened emergence time, and a lower ratio of EEG-δ. Our findings revealed a role of brain glycogen in regulating anesthesia-arousal, providing a potential target for modulating anesthesia.

6.
Molecules ; 25(20)2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33050458

RESUMO

Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties. To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays. The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting. The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B. Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time. The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 µg/mL EEP-B. This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression. These findings could provide a theoretical basis for MAEC treatment using EEP-B.

7.
Acta Pharmacol Sin ; 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028984

RESUMO

Sustained elevation of corticosterone (CORT) is one of the common causes of aging and major depression disorder. However, the role of elevated CORT in late life depression (LLD) has not been elucidated. In this study, 18-month-old female rats were subjected to bilateral adrenalectomy or sham surgery. Their CORT levels in plasma were adjusted by CORT replacement and the rats were divided into high-level CORT (H-CORT), low-level CORT (L-CORT), and Sham group. We showed that L-CORT rats displayed attenuated depressive symptoms and memory defects in behavioral tests as compared with Sham or H-CORT rats. Furthermore, we showed that glutamatergic transmission was enhanced in L-CORT rats, evidenced by enhanced population spike amplitude (PSA) recorded from the dentate gyrus of hippocampus in vivo and increased glutamate release from hippocampal synaptosomes caused by high frequency stimulation or CORT exposure. Intracerebroventricular injection of an enzymatic glutamate scavenger system, glutamic-pyruvic transmine (GPT, 1 µM), significantly increased the PSA in Sham rats, suggesting that extracelluar accumulation of glutamate might be the culprit of impaired glutamatergic transmission, which was dependent on the uptake by Glt-1 in astrocytes. We revealed that hippocampal Glt-1 expression level in the L-CORT rats was much higher than in Sham and H-CORT rats. In a gradient neuron-astrocyte coculture, we found that the expression of Glt-1 was decreased with the increase of neural percentage, suggesting that impairment of Glt-1 might result from the high level of CORT contributed neural damage. In sham rats, administration of DHK that inhibited Glt-1 activity induced significant LLD symptoms, whereas administration of RIL that promoted glutamate uptake significantly attenuated LLD. All of these results suggest that glutamatergic transmission impairment is one of important pathogenesis in LLD induced by high level of CORT, which provide promising clues for the treatment of LLD.

8.
Clin Cardiol ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33022118

RESUMO

BACKGROUND: The development of a perfect method for determining the mean QRS axis (ÂQRS) is still lacking. HYPOTHESIS: We proposed a new simple method, whether this method is accurate is unknown. METHODS: The axis perpendicular to the mean QRS axis (P-ÂQRS) divides six limb leads into two groups. All the leads that are in the range of 180° along the ÂQRS are positive, while all the leads in another 180° are negative, one lead is isodiphasic if it is on the P-ÂQRS. If no lead is isodiphasic, then the P-ÂQRS is located in the middle of two adjacent leads, which can help us determine the P-ÂQRS. The six limb leads that fall in the range of -30° to 120° are as follows: aVL (-30°), I (0°), -aVR (30°), II (60°), aVF (90°), and III (120°). We can check an external lead (aVL or III) first. For example, if lead III is isodiphasic and lead aVF is positive, the P-ÂQRS is 120°; if lead III is negative and lead aVF is positive, then the P-ÂQRS is 105°. If more than one lead is negative, all such leads can be checked individually until a positive or isodiphasic lead is found. The ÂQRS can be easily decided once we know the P-ÂQRS. In total, 200 recorded ECGs were investigated. We obtained the ÂQRS from our new method, computer interpretations, and a standard bipolar method. The Pearson correlation coefficient and Bland-Altman analysis were performed. RESULTS: The mean and SDs were remarkably similar, the correlation coefficient between the P-ÂQRS method and the bipolar method was 0.976 (P < .001). Mean bias (Bland-Altman limits of agreement) between the two methods was 0.885 (-12.37 to 14.14). CONCLUSION: The new method is simple and is able to assess the mean QRS axis accurately.

9.
Steroids ; 164: 108734, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33010265

RESUMO

A new withanolide, tubocaapsanolide MAP (MeOH addition product) (1), as well as its known precursor tubocaapsanolide A (2) were obtained from Tubocapsicum anomalum (Solanaceae). Compound 1 was a MeOH addition product transformed from compound 2 during the process of separation using MeOH as solvent. The structures of the two withanolides including absolute configuration were elucidated by extensive spectroscopic analysis and X-ray single crystal diffraction. In the test of anti-triple negative breast cancer (TNBC) effects, tubocapsusanlide A (2) showed potent inhibitory activity against four human TNBC cell lines, while tubocapsusanlide MAP (1) exhited significantly weaker inhibitory than that of tubocapsusanlide A (2), indicating that α-ß unsaturated carbonyl unit contained in 2 was closely related to its anti-TNBC activity. The potent bioactivity displayed significant developing potential of withanolides as anti-TNBC lead compounds or drug candidates. And this report may provide some useful guidances for the preparation and bioactivity research of withanolides.

10.
Neuroreport ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33044327

RESUMO

Recent structural MRI studies on gray matter (GM) volumes using group-level mass-univariate statistical analysis suggest that chronic and heavy cannabis exposure may affect brain region-based morphology. In this prospective study, we use a multivariate pattern analysis approach to investigate the voxel-level change of GM densities in chronic heavy cannabis users. Principal component analysis and linear support vector machine are used in this study, resulting in an 88.1% separation between chronic heavy cannabis users (N = 20) and non-cannabis healthy controls (HCs, N = 22) through leave-one-out cross-validation. The model's discriminative pattern showed that GM density decreases in the part of middle frontal gyrus, inferior frontal gyrus, middle temporal gyrus, inferior temporal gyrus and left occipital lobe in heavy cannabis users with respect to HCs and increases in the part of lentiform nucleus, left cerebellum and right parietal lobe. These results suggest that GM densities alteration has taken place on chronic heavy cannabis users compared with HCs at voxel level.

11.
Biomed Res Int ; 2020: 3516128, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33029504

RESUMO

Ulcerative colitis is one of the IBD which cause a chronic intestinal inflammation and dysfunctional of the mucosal barrier. For now, the incident of UC was steadily increased all over the world. It has become a novel independent risk factor of several severe diseases especially colon-rectal cancer. However, the etiology of UC was still obscure. Previous studies show that high-fat diet contributed to the pathogenesis of immune system dysregulation, and farnesoid X receptor (FXR) was also implicated in the pathogenesis of various inflammatory symptoms. Yet, their inner roles in the pathogenesis of UC have not been mentioned. In this study, we aim to investigate the role of FXR in UC. High-fat diet (HFD) promotes the progression of DSS-induced UC, shows an increasing secretion of bile acid in serum, and leads to a downregulation of FXR target genes (FXRα, Shp, and lbabp). Adding FXR agonist FexD rescues the phenotype induced by high-fat diet, whereas TGFBRI inhibitor SB431542 abrogates the restoration by FexD in DSS-induced UC mice. To further verify the relationship between the FXR and TGFB signaling pathway, we made a UC-HFD model in the Caco2 cell line. Results shows the same conclusion that FXR mitigate UC inflammation through a TGFB-dependent pathway. These results expand the role of FXR in ulcerative colitis and suggest that FXR activation may be considered a therapeutic strategy for UC.

12.
Nucleic Acids Res ; 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33010159

RESUMO

Proteolysis-targeting chimeras (PROTACs), which selectively degrade targeted proteins by the ubiquitin-proteasome system, have emerged as a novel therapeutic technology with potential advantages over traditional inhibition strategies. In the past few years, this technology has achieved substantial progress and two PROTACs have been advanced into phase I clinical trials. However, this technology is still maturing and the design of PROTACs remains a great challenge. In order to promote the rational design of PROTACs, we present PROTAC-DB, a web-based open-access database that integrates structural information and experimental data of PROTACs. Currently, PROTAC-DB consists of 1662 PROTACs, 202 warheads (small molecules that target the proteins of interest), 65 E3 ligands (small molecules capable of recruiting E3 ligases) and 806 linkers, as well as their chemical structures, biological activities, and physicochemical properties. Except the biological activities of warheads and E3 ligands, PROTAC-DB also provides the degradation capacities, binding affinities and cellular activities for PROTACs. PROTAC-DB can be queried with two general searching approaches: text-based (target name, compound name or ID) and structure-based. In addition, for the convenience of users, a filtering tool for the searching results based on the physicochemical properties of compounds is also offered. PROTAC-DB is freely accessible at http://cadd.zju.edu.cn/protacdb/.

13.
Acta Pharmacol Sin ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33024240

RESUMO

DJ-1 is a multifunctional protein associated with cancers and autosomal early-onset Parkinson disease. Besides the well-documented antioxidative stress activity, recent studies show that DJ-1 has deglycation enzymatic activity and anti-ferroptosis effect. It has been shown that DJ-1 forms the homodimerization, which dictates its antioxidative stress activity. In this study, we investigated the relationship between the dimeric structure of DJ-1 and its newly reported activities. In HEK293T cells with Flag-tagged and Myc-tagged DJ-1 overexpression, we performed deletion mutations and point mutations, narrowed down the most critical motif at the C terminus. We found that the deletion mutation of the last three amino acids at the C terminus of DJ-1 (DJ-1 ΔC3) disrupted its homodimerization with the hydrophobic L187 residue being of great importance for DJ-1 homodimerization. In addition, the ability in methylglyoxal (MGO) detoxification and deglycation was almost abolished in the mutation of DJ-1 ΔC3 and point mutant L187E compared with wild-type DJ-1 (DJ-1 WT). We also showed the suppression of erastin-triggered ferroptosis in DJ-1-/- mouse embryonic fibroblast cells was abolished by ΔC3 and L187E, but partially diminished by V51C. Thus, our results demonstrate that the C terminus of DJ-1 is crucial for its homodimerization, deglycation activity, and suppression of ferroptosis.

14.
J Environ Manage ; 277: 111439, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33035939

RESUMO

Overgrazing is the main driver of grassland degradation and productivity reduction in northern China. The restoration of degraded grasslands depends on optimal grazing regimes that modify the source-sink balance to promote best carbon (C) assimilation and allocation, thereby promoting rapid compensatory growth of the grazed plants. We used in situ13CO2 labeling and field regrowth studies of Stipa grandis P.A. Smirn.to examine the effects of different grazing intensities (light, medium, heavy, and grazing exclusion) on photosynthetic C assimilation and partitioning, on reallocation of non-structural carbohydrates during regrowth, and on the underlying regulatory mechanisms. Light grazing increased the sink demand of newly expanded leaves and significantly promoted 13C fixation by increasing the photosynthetic capacity of the leaves and accelerating fructose transfer from the stem. Although C assimilation decreased under medium and heavy grazing, S. grandis exhibited a tolerance strategy that preferentially allocated more starch and 13C to the roots for storage to balance sink competition between newly expanded leaves and the roots. Sucrose phosphate synthase (SPS), sucrose synthase (SS), and other plant hormones regulated source-sink imbalances during regrowth. Abscisic acid promoted accumulation of aboveground biomass by stimulating stem SPS activity, whereas jasmonate increased root starch synthesis, thereby increasing belowground biomass. Overall, S. grandis could optimize source-sink relationships and above- and belowground C allocation to support regrowth after grazing by the regulating activities of SPS, SS and other hormones. These results provide new insights into C budgets under grazing and guidance for sustainable grazing management in semi-arid grasslands.

15.
Chem Asian J ; 2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-32865315

RESUMO

Metastable metallic phases of transition-metal dichalcogenide (TMD) nanomaterials have displayed excellent performance and emerged as promising candidates for sustainable energy sources low-cost storage and conversion because of their two-dimensional (2D) layered structures and extraordinary physicochemical properties. In order to broaden the range of potential applications, defect engineering is applied to the metastable phases of TMDs for further improvement of their catalytic and electronic properties. According to some recent studies, effective introduction of defects without perturbing the interior conductivity contributes to the development of metastable TMDs. This review provides deep insights into recent progress in electrochemistry using defect engineering in the metastable phases of TMDs. After introducing the structures of metastable phases and methods for defect construction, significant developments in catalysis and energy storage applications are discussed to elucidate structure-function relationships. Key challenges and future directions for defect engineering in the metastable phases of TMDs are also highlighted in the conclusions.

16.
J Food Biochem ; : e13482, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32964487

RESUMO

Two new water-soluble polysaccharide fractions (SCP II-1 and SCP II-2) were obtained from silkworm cordycepsusing DEAE Sepharose FF and Superose 6 columns chromatography. The characterization of their basic structure was studied by high performance liquid chromatography, high performance ion chromatography, infrared spectroscopy and atomic force microscope (AFM). The results showed that the molecular weight of SCP II-1 and SCP II-2 were 35.2 kDa and 23.4 kDa, and they were mainly composed of ribose, mannose, glucose, and galactose in molar ratio of 1.0:27.38:8.52:17.99 and 1.0:21.21:1.95:14.28, respectively. The AFM topography confirmed the highly branched chain conformation of SCP II-1, while SCP II-2 had more polymerized chain morphology. These two fractions possessed excellent antioxidant and antitumor activities, especially SCP II-1 showed better inhibition than SCP II-2. Those data suggested that purified polysaccharides from silkworm cordyceps have potential application in functional food and pharmaceutical industry. PRACTICAL APPLICATIONS: Silkworm cordyceps is cultivated on the 5th instar larvae inoculated with Cordyceps sp. and the functions of cordyceps have been reported recently. The separation and purification of silkworm cordyceps polysaccharide is helpful to better exert important biological functions. The study on the structure-function relations of polysaccharides will be useful to the application of polysaccharides in functional food and pharmaceutical industry.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32915724

RESUMO

The key to the effective control of a diffusion system lies in how accurately we could predict its unfolding dynamics based on the observation of its current state. However, in the real-world applications, it is often infeasible to conduct a timely and yet comprehensive observation due to resource constraints. In view of such a practical challenge, the goal of this work is to develop a novel computational method for performing active observations, termed active surveillance, with limited resources. Specifically, we aim to predict the dynamics of a large spatio-temporal diffusion system based on the observations of some of its components. Towards this end, we introduce a novel measure, the γ value, that enables us to identify the key components by means of modeling a sentinel network with a row sparsity structure. Having obtained a theoretical understanding of the γ value, we design a scalable Sentinel Network Mining Algorithm (SNMA) for deriving the sentinel network that could involve complex diffusion mechanisms via group sparse Bayesian learning. We show the effectiveness of SNMA by validating it using both synthetic datasets and five real-world datasets. The experimental results are appealing, which demonstrate that SNMA readily outperforms the state-of-the-art methods.

18.
Virchows Arch ; 2020 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-32918598

RESUMO

To examine the clinicopathologic and immunohistochemical features of a group of newly defined low-grade oncocytic renal tumors (LOT) that have the "CD117 negative/cytokeratin (CK)7 positive" immunoprofile. We have queried our hospital database and found 4456 consecutive renal tumors between 2016 and 2019. Among these renal tumors, eight (8) cases meet the morphologic and immunohistochemical characterization for low-grade oncocytic renal tumor (LOT). The eight (8) patients' mean age is 56.6 years (range 39-70 years old), and the male to female ratio is 1:1. Macroscopically, these LOTs generally present with tan-brown and solid cut surfaces and demonstrate similar solid, compact nested growth pattern microscopically. Tumor cells exhibit oncocytic cytoplasm and uniformly rounded to oval nuclei. There are areas of edematous stroma containing dispersed single or small clustered tumor cells. All tumors are negative for CD117 and positive for CK7. Uniform reactivity is also found for BerEP4, cyclin D1, and SDHB. Besides, CD10, vimentin, and AMACR are either negative or only focally positive. All of the tumors are negative for CA9 and TFE. The Ki-67 index is less than 5% in the seven (7) internal cases. Seven (7) of the eight (8) patients who are available for follow-up are alive and without disease recurrence (mean follow-up period of 21.6 months, ranging from 6 to 43 months). We described a group of low-grade oncocytic renal tumors identified retrospectively in a large tertiary cancer center, which was probably the first report originated from China or even Asia in the English literature so far. These tumors demonstrated eosinophilic cytoplasm and low-grade appearing nuclei with a "CD117 negative/CK7 positive" immunoprofile. The incidence rate was about 3.7% of the oncocytic renal tumors and 0.18% of all the renal tumors that were received in our lab during the four-year period. It is necessary to separate this group of tumors by its characteristic morphologic and immunophenotypic features.

19.
PLoS One ; 15(9): e0238179, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32881902

RESUMO

Carotenoid cleavage dioxygenase (CCD), a key enzyme in carotenoid metabolism, cleaves carotenoids to form apo-carotenoids, which play a major role in plant growth and stress responses. CCD genes had not previously been systematically characterized in Brassica napus (rapeseed), an important oil crop worldwide. In this study, we identified 30 BnCCD genes and classified them into nine subgroups based on a phylogenetic analysis. We identified the chromosomal locations, gene structures, and cis-promoter elements of each of these genes and performed a selection pressure analysis to identify residues under selection. Furthermore, we determined the subcellular localization, physicochemical properties, and conserved protein motifs of the encoded proteins. All the CCD proteins contained a retinal pigment epithelial membrane protein (RPE65) domain. qRT-PCR analysis of expression of 20 representative BnCCD genes in 16 tissues of the B. napus cultivar Zhong Shuang 11 ('ZS11') revealed that members of the BnCCD gene family possess a broad range of expression patterns. This work lays the foundation for functional studies of the BnCCD gene family.

20.
Artigo em Inglês | MEDLINE | ID: mdl-32980146

RESUMO

OBJECTIVE: The study objective was to provide evidence for choosing a bioprosthesis in treating patients with active aortic valve endocarditis. METHODS: From 1998 to 2017, 265 patients with active aortic valve endocarditis underwent aortic valve replacement with a stented valve (n = 97, 37%) or a stentless valve (n = 168, 63%) with further breakdown into inclusion technique (n = 142, 85%) or total root replacement (n = 26, 15%). Data were obtained from the Society of Thoracic Surgeons database aided with chart review, surveys, and National Death Index data. RESULTS: The median age of patients was 53 years (43-56) in the stented group and 57 years (44-66) in the stentless group. The stented and stentless groups had high rates of heart failure (54% and 40%), liver disease (16% and 7.7%), prosthetic valve endocarditis (14% and 48%), root abscess (38% and 70%), and concomitant ascending aorta procedures (6.2% and 22%), respectively. The stentless group required permanent pacemakers in 11% of cases. Operative mortality was similar between groups (6.2% and 7.1%). The 5-year survival was 52% and 63% in the stented and stentless groups, respectively. Significant risk factors for long-term mortality included liver disease (hazard ratio, 2.38), previous myocardial infarction (hazard ratio, 1.64), congestive heart failure (hazard ratio, 1.63), and renal failure requiring dialysis (hazard ratio, 4.37). The 10-year cumulative incidence of reoperation was 12% and 3.4% for the stented and stentless groups, respectively. The 10-year freedom from reoccurrence of aortic valve endocarditis was 88% for the stented and 98% for the stentless groups. CONCLUSIONS: Both stented and stentless aortic valves are appropriate conduits for replacement of active aortic valve endocarditis for select patients.

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