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1.
Mater Sci Eng C Mater Biol Appl ; 106: 110161, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31753384

RESUMO

Solid inclusion complexes between chrysin and four amino-appended ß-cyclodextrins (ACDs) were prepared by suspension method and characterized in solid and solution states by kinds of analytical methods. The scanning electron microscopy (SEM) showed distinct micro-morphologies of them. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis revealed their unique thermal properties, such as decomposition temperatures and endothermic points. Powder X-ray diffractometry (XRD) analysis disclosed their unique crystal patterns. Their nuclear magnetic resonance (NMR) analyses provided the variations of chemical shifts before and after the formation of inclusion complexes. Their binding stability constants (Ks) were 574, 842, 704, and 474 L·mol-1, respectively, as determined by spectral titration. A 1:1 inclusion mode with self-assembly of their amino side chains inside the ACD cavity was proposed based on Job plot and 2D-ROESY experiments. Water solubility of chrysin was promoted up to 4411.98 µg·mL-1 after formation of inclusion complexes with ACDs, better than that of ß-CD and its derivatives, i.e., HP- and SBE-ß-CD. In vitro antioxidant activity of chrysin was also improved after inclusion complexation by the DPPH scavenging assay. Furthermore, in vitro cytotoxicity of solid inclusion complexes towards three human cancer cell lines, A549, HT-29 and HCT116 were enhanced significantly.

2.
Methods Mol Biol ; 2064: 125-134, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31565771

RESUMO

Imaging mass spectrometry is a powerful technology that combines the molecular measurements of mass spectrometry with the spatial information inherent to microscopy. This unique combination of capabilities is ideally suited for the analysis of metabolites and lipids from single cells. This chapter describes a methodology for the sample preparation and analysis of single cells using high performance MALDI FTICR MS. Using this approach, we are able to generate profiles of lipid and metabolite expression from single cells that characterize cellular heterogeneity. This approach also enables the detection of variations in the expression profiles of lipids and metabolites induced by chemical stimulation of the cells. These results demonstrate that MALDI IMS provides an insightful view of lipid and metabolite expression useful in the characterization of a number of biological systems at the single cell level.

3.
Sci Total Environ ; 701: 134721, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31715478

RESUMO

Although epidemiological studies have evaluated the associations of ambient air pollution with depression, the results remained mixed. To clarify the nature of the association, we performed a comprehensive systematic review and meta-analysis with the Inverse Variance Heterogeneity (IVhet) model to estimate the effect of ambient air pollution on depression. Three English and four Chinese databases were searched for epidemiologic studies investigating associations of ambient particulate (diameter ≤ 2.5 µm (PM2.5), ≤10 µm (PM10)) and gaseous (nitric oxide (NO), nitrogen dioxide (NO2), carbon monoxide (CO), sulfur dioxide (SO2) and ozone (O3)) air pollutants with depression. Odds ratios (OR) and corresponding 95% confidence intervals (CI) were calculated to evaluate the strength of the associations. We identified 22 eligible studies from 10 countries of the world. Under the IVhet model, per 10 µg/m3 increase in long-term exposure to PM2.5 (OR: 1.12, 95% CI: 0.97-1.29, I2: 51.6), PM10 (OR: 1.04, 95% CI: 0.88-1.25, I2: 85.7), and NO2 (OR: 1.05, 95% CI: 0.83-1.34, I2: 83.6), as well as short-term exposure to PM2.5 (OR: 1.01, 95% CI: 0.99-1.04, I2: 51.6), PM10 (OR: 1.01, 95% CI: 0.98-1.04, I2: 86.7), SO2 (OR: 1.03, 95% CI: 0.99-1.07, I2: 71.2), and O3 (OR: 1.01, 95% CI: 0.99-1.03, I2: 82.2) was not significantly associated with depression. However, we observed significant association between short-term NO2 exposure (per 10 µg/m3 increase) and depression (OR: 1.02, 95% CI: 1.00-1.04, I2: 65.4). However, the heterogeneity was high for all of the pooled estimates, which reduced credibility of the cumulative evidence. Additionally, publication bias was detected for six of eight meta-estimates. In conclusion, short-term exposure to NO2, but not other air pollutants, was significantly associated with depression. Given the limitations, a larger meta-analysis incorporating future well-designed longitudinal studies, and investigations into potential biologic mechanisms, will be necessary for a more definitive result.

4.
Food Chem ; 303: 125399, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31470274

RESUMO

It is still a challenge to solve the matrix interferences in veterinary drug residue analysis. In this study, we reported a thin layer chromatography (TLC)-high-performance liquid chromatography (HPLC) method for determining total florfenicol (FF) residues, expressed as florfenicol amine (FFA), in porcine edible tissues. The tissue homogenate were acid-hydrolyzed to liberate the bound residues and convert them into FFA. The hydrolysates were washed with ethyl acetate and subsequently extracted with ethyl acetate under alkaline conditions. The supernatants were concentrated through evaporation, defatted with hexane, purified by TLC and analyzed by HPLC at 225 nm. The optimal developing solvent for TLC purification was ethyl acetate-acetone-ammonium hydroxide mixtures (2:8:0.5, v/v/v). The method was fully validated according to decision 2002/657/EC, and could be used for the routine monitoring of FF residues in pig. TLC showed excellent purification efficiency, and was expected to solve the matrix interferences in veterinary drug residue analysis.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Cromatografia em Camada Delgada/métodos , Resíduos de Drogas/análise , Tianfenicol/análogos & derivados , Drogas Veterinárias/análise , Estruturas Animais/química , Animais , Cromatografia Líquida/métodos , Carne/análise , Suínos , Tianfenicol/análise
5.
Chemosphere ; 239: 124660, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31505445

RESUMO

The co-precipitation of Fe2+, Cu2+, Zn2+, Cd2+ and Ni2+ were investigated by a mechanochemical processing with CaCO3. The results showed that the synergies of the metal ions led to efficient co-precipitation. The precipitation of Fe2+, Cu2+ and Cd2+ are over 99% and that of Zn2+ and Ni2+ about 98.4% and 93.8%. A significant advantage of the process is that the moisture content of filter residue is much lower (less than 50%) than that using the lime neutralization (more than 80%), offering a potential solution to the sludge problem in wastewater treatment. A further advantage is the neutral pH (about 7.5) obtained by using CaCO3 rather than the highly alkaline pH (about 11) obtained using lime (Ca(OH)2) neutralization method.

6.
Chemosphere ; 240: 124949, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31568949

RESUMO

Pharmaceutically active compounds are of great concern due to their detection frequency in the environment and the unexpected risks. In this study, the simultaneous removal of mixed pharmaceuticals by microalgae was explored using a typical freshwater diatom Navicula sp. Results showed that Navicula sp. could efficiently remove atenolol, carbamazepine, ibuprofen and naproxen with the efficiencies of >90% after 21 d of exposure. As compared to the removal efficiencies of each pharmaceutical in the individual pharmaceutical treatments, the degradation of sulfamethoxazole, bezafibrate, and naproxen was improved in the mixed treatment, whereas the removal efficiencies of carbamazepine and atenolol decreased. Additionally, the presence of hydrophobic pharmaceuticals (i.e., ibuprofen and naproxen) accelerated the degradation of carbamazepine and sulfamethoxazole and inhibited the removal of atenolol in the mixture with the combination of six pharmaceuticals, while the addition of other pharmaceuticals show no significant effect on the removal of ibuprofen and naproxen. The bioaccumulation of pharmaceuticals in Navicula sp. increased as their log KOW values decreased. Four bezafibrate metabolites were identified and the degradation pathways of bezafibrate in diatom were proposed. It is the first report on the metabolism of BEZ in diatom, and further studies on the environmental risk of the metabolites should be investigated.

7.
Biochim Biophys Acta Gen Subj ; 1864(1): 129397, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31306710

RESUMO

BACKGROUND: Yes-associated protein (YAP) is a key effector of the Hippo pathway and is frequently dysregulated in aggressive human cancers. Aberrant YAP activation has emerged as an important driver of tumorigenesis, chemoresistance and metastasis. Since posttranslational modifications (PTMs) are pivotal modifiers that determine protein activation or subcellular localization, the malfunction of YAP due to dysregulated PTMs has been linked to various cancers. Collectively, although YAP has long been considered an "undruggable" transcription cofactor, its PTMs may be its "Achilles' heel". To provide theoretical support for developing small molecule inhibitors based on PTMs, in this review article, we summarize the current understanding of the impact of PTMs in regulating the Hippo-YAP pathway and further discuss potential therapeutic intervention. SCOPE OF REVIEW: In our review, we summarize the known posttranslational modifications (PTMs) of YAP that dictate its protein stability, transcriptional activity and subcellular localization at different stages. Here, we clearly summarize the specific enzymes and sites involved in YAP PTMs and place additional focus on the consequences of PTM-modulated YAP activity and translocation. MAIN CONCLUSION: PTMs of YAP play fundamental roles in controlling the protein abundance and function. Therefore, interfering with PTMs of YAP may contribute to solving the "undruggable" problem in YAP inhibition, thus providing new approaches for YAP-based cancer therapy. GENERAL SIGNIFICANCE: Future studies that target corresponding PTM-related kinases/enzymes will provide new strategies for cancer therapy, particularly in tumors with YAP dysregulation.

8.
Biomaterials ; 229: 119580, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31707296

RESUMO

Hypoxia, which frequently reduces the sensitivity to many therapeutic interventions, including chemotherapy, radiotherapy and phototherapy, has been acknowledged as an important reason for poor prognosis. Burgeoning evidences have proved that the tumor hypoxia microenvironment can reduce the therapeutic effect on tumor through inhibiting the drug efficacy, limiting immune cell infiltration of tumors and accelerating tumor recurrence and metastasis. However, the relationship between oxygen supply and the proliferation of cancer cells is still ambiguous and argued. Different from the current commonly used oxygen supply strategies, this study concentrated on the reduction of endogenous oxygen consumption. Specifically, a novel photosensitizers (IR780) and metformin are packaged in PEG-PCL liposomes. Once such nanoparticles accumulated in tumor tissues, the tumor foci were irradiated through 808 nm laser, generated ROS to further release metformin and IR780. Metformin can directly inhibit the activity of complex Ⅰ in the mitochondrial electron transport chain, thus performed a potent inhibitor of cell respiration. After overcoming tumor hypoxia, the combination of mitochondria-targeted photodynamic therapy (PDT) and photothermic therapy (PTT) via IR780 may achieve superior synergistically therapeutic efficacy. Benefit from excellent characteristics of IR780, such synergistic PDT PTT with the inhibition of mitochondrial respiration can be monitored through near-infrared/photoacoustic dual-modal imaging. Such a conception of reducing endogenous oxygen consumption may offer a novel way to solve the important puzzles of hypoxia-induced tumor resistance to therapeutic interventions, not limited to phototherapy.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31792793

RESUMO

Leaf vegetables serve as an important food for the local residents in China. This paper focuses on the uptake, accumulation, transfer, and mercury (Hg) sensitivity of leafy vegetables. Two types of soil (an alkaline Cambosol and an acid Ferrosol) and eleven species of leafy vegetable, namely, Spinach, Tung choy, Leek, Fennel, Coriander, Chinese flowering cabbage, Wuta-tsai, Pakchoi, Chicory, Crown daisy, and Lettuce, were selected to investigate their sensitivity to Hg accumulation in a greenhouse pot experiment. Three Hg concentration treatments were carried out as control (background values), low concentration (1.5 times standard value), and high concentration (2 times standard value) as adjusted by the soil pH. Hg concentrations of more than half vegetable samples grown in Cambosol (collected from Shandong Province) reached or exceeded the maximum permissible food safety levels (10 µg kg-1) according to the General Standard of Contaminants in Food in China (GB 2762-2012), while only about 15% in Ferrosol (collected from Jiangxi Province). Meanwhile, Hg bio-concentration factors (BCF) in all treatments were < 1, while Hg translocation factors (TF) in most treatments were < 1. Correlation analysis among soil, root, and edible plant parts revealed that the principal source of Hg in leafy vegetables was most likely from Hg-contaminated soils. Species sensitivity distribution (SSD) models were constructed and their simulated curves indicated that sensitivity to Hg was highest in Pakchoi in low Hg-contaminated soils, and Chicory in highly Hg-contaminated soils. Therefore, Hg concentration is mostly accumulated in roots of leafy crops, which reduces the risk of Hg bioaccumulation in edible portion of vegetables, and (2) Brassicaceae vegetables are mostly less sensitive to soil Hg contamination. Our results provide effective guidance for the selection of leafy vegetables for cultivation and daily consumption that minimizes health risk.

10.
J Dig Dis ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31794121

RESUMO

AIMS: The prevalence of inflammatory bowel disease (IBD) has been increasing worldwide, and the risk of infection has increased due to the use of immunosuppressive and biologic medications. Some of these infections can be prevented with vaccinations. The aim of this study was to evaluate the vaccination practices of Chinese gastroenterologists for IBD patients. METHODS: Questionnaires based on quick response code were distributed using e-mail and the WeChat platform to gastroenterologists at 20 hospitals in China. The vaccination practices of the gastroenterologists, including for hepatitis B, hepatitis A, and varicella, were assessed. RESULTS: Of the 468 gastroenterologists who received the questionnaire, 307 (65.6%) completed it. The gastroenterologists were most concerned with hepatitis B; 83.4% always or frequently took an infection history, 53.7% took an immunization history, and 73.6% tested for hepatitis B infection. However, few gastroenterologists did so for hepatitis A or varicella. The proportion of patients who took an infection history and immunization history, and tested for varicella infection, was 16%, 15%, and 9.4%, respectively. Only a few gastroenterologists recommended vaccination for infection-negative patients before IBD medical treatment (26.7% for hepatitis A, 45.6% for hepatitis B, and 28% for varicella vaccination). CONCLUSIONS: Vaccination practices for IBD patients engaged in by Chinese gastroenterologists varied greatly, suggesting that education regarding immunization is needed. This article is protected by copyright. All rights reserved.

11.
Mar Pollut Bull ; : 110693, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31753563

RESUMO

Fourteen field cruises were carried out in a mariculture region of the northern Shandong Peninsula, North Yellow Sea, China from 2016 to 2017 for a better understanding of the biogeochemical behaviors, sources and export of dissolved inorganic nutrients. The spatial variations of nutrients were not obvious due to the influence of complex hydrological and biochemical conditions. Potential nutritional level was characterized in oligotrophy, and trophic status was rated at medium level. A preliminary estimation of nutrient budgets demonstrated that the dissolved inorganic nitrogen (DIN) load was mainly from atmospheric deposition and scallop excretion, accounting for 56.9% and 35.6% of its total influx. Scallop excretion and sediment release were the major source of phosphate (DIP), contributing to 25.2% and 44.3%, while dissolved silicon (DSi) was mainly from sediment release, accounting for 94.2%. In addition, about 136.7 × 103, 7.3 × 103 and 485.5 × 103 mol km-2 yr-1 of DIN, DIP and DSi could be converted into other forms, e.g. organic and particulate matter and gas species.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31753642

RESUMO

Raman spectra of water/dimethyl sulfoxide (DMSO) mixtures have been observed at room temperature and atmospheric pressure. We find that the Raman peaks corresponding to the symmetric and asymmetric O-H stretching vibration mode of water rapidly move to lower wavenumber with increasing DMSO concentration. These results indicate that the strong hydrogen bond between DMSO-water complexes helps to strengthen the tetrahedral structure of water when the volume fraction of DMSO is less than 0.6. Moreover, the blue/red shifts of SO and C-H are obvious when the concentration of DMSO reaches 0.6, which may be due to changes in the structure of the DMSO-water complex. Furthermore, the frequency shift of the C-H group indicates that the non-polar methyl group of DMSO forms a hydrophobic hydrated structure. Finally, the frequency shift of the Raman peaks of SO and C-H exhibited a highly consistent concentration dependence due to the cooperation effect of the C-H⋯O with the O-H⋯OS.

13.
ACS Nano ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31769966

RESUMO

Spinal cord injury (SCI) is one of the most debilitating injuries, and transplantation of stem cells in a scaffold is a promising strategy for treatment. However, stem cell treatment of SCI has been severely impaired by the increased generation of reactive oxygen species in the lesion microenvironment, which can lead to a high level of stem cell death and dysfunction. Herein, a MnO2 nanoparticle (NP)-dotted hydrogel is prepared through dispersion of MnO2 NPs in a PPFLMLLKGSTR peptide modified hyaluronic acid hydrogel. The peptide-modified hydrogel enables the adhesive growth of mesenchymal stem cells (MSCs) and nerve tissue bridging. The MnO2 NPs alleviate the oxidative environment, thereby effectively improving the viability of MSCs. Transplantation of MSCs in the multifunctional gel generates a significant motor function restoration on a long-span rat spinal cord transection model and induces an in vivo integration as well as neural differentiation of the implanted MSCs, leading to a highly efficient regeneration of central nervous spinal cord tissue. Therefore, the MnO2 NP-dotted hydrogel represents a promising strategy for stem-cell-based therapies of central nervous system diseases through the comprehensive regulation of pathological microenvironment complications.

14.
J Cell Biochem ; 2019 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-31736127

RESUMO

Long noncoding RNAs (lncRNAs) are recently recognized as noteworthy regulators of different tumors, counting gastric cancer (GC). Lately, long intergenic noncoding RNA (LINC) 00665 has been verified to display significant parts in several cancers. Be that as it may, its role and mechanism in GC movement still stay uninvestigated. As of now, we observed LINC00665 was obviously GC cells (MKN28, BGC-823, SGC7-901, AGS, HGC-27) in comparison to GES-1 cells, which was identified as human normal gastric epithelial cells. Then, LINC00665 was obviously downregulated in GC cells including AGS and BGC-823 cells. Loss of LINC00665 greatly repressed AGS and BGC-823 cell survival and cell expansion. Moreover, GC cell apoptosis was significantly induced by the loss of LINC00665. For another, we found that the GC cell cycle was also captured in G1 and G2 phases. The experiments on cell migration and invasion indicated that knockdown of LINC00665 restrained GC cell migration and invasion. Modifications in Wnt signaling are closely associated with the development of cancers. Here, we found that Wnt signaling was significantly inactivated by the silence of LINC00665 in GC cells. ß-catenin and cyclinD1 were restrained whereas GSK-3ß was induced by the inhibition of LINC00665 in GC cells. Furthermore, we confirmed the impact of LINC00665 in vivo using xenograft models. Taken these together, we indicated that LINC00665 could function as a novel biomarker in GC progression.

15.
J Virol ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666381

RESUMO

Infectious bursal disease virus (IBDV) is an important member of the Birnaviridae family, causing severe immunosuppressive disease in chickens. The major capsid VP2 protein is responsible for the binding of IBDV to the host cell and its cellular tropism. In order to find the potential interaction proteins with IBDV VP2, LC/MS assay was conducted and host protein chicken CD74 protein was identified. Here, we investigate the role of chicken CD74 in IBDV attachment. Co-immunoprecipitation assays indicated that the extracellular domain of CD74 interacted with the VP2 protein of multiple IBDV strains. Knockdown and Overexpression experiments showed that CD74 promotes viral infectivity. Confocal assay showed that CD74 overexpression allows the attachment of IBDV and subvirus-like particles (SVPs) to the cell surface of non-permissive cells, and qPCR analysis further confirmed the attachment function of CD74. Anti-CD74 antibody, soluble CD74, depletion of CD74 by small interfering RNA (siRNA), and CD74 knockdown in the IBDV-susceptible DT40 cell line significantly inhibited IBDV binding, suggesting a pivotal role of this protein in virus attachment. These findings demonstrate that CD74 is a novel important receptor for IBDV attachment to the chicken B lymphocyte cell line DT40.IMPORTANCE CD74 plays a pivotal role in the correct folding and functional stability of MHC II molecules and in the presentation of antigenic peptides, acting as a regulatory factor in the antigen presentation process. In our study, we demonstrated a novel role of CD74 during IBDV infection, showing that chicken CD74 plays a significant role in IBDV binding to target B cells by interacting with the viral VP2 protein. This is the first report demonstrating that CD74 is involved as a novel attachment receptor in the IBDV life cycle in target B cells, thus contributing new insight into host-pathogen interactions.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31669033

RESUMO

OBJECTIVE: The study objective was to evaluate the management of malperfusion in acute type B aortic dissection with endovascular fenestration/stenting. METHODS: From 1996 to 2018, 182 patients with an acute type B aortic dissection underwent fenestration/stenting for suspected malperfusion based on imaging, clinical manifestations, and laboratory findings. Data were obtained from medical record review and the National Death Index database. RESULTS: The median age of patients was 55 years. Signs of malperfusion included abdominal pain (61%), lower-extremity weakness (27%), nonpalpable lower-extremity pulses (24%), and abnormal lactate, creatinine, liver enzymes, and creatine kinase levels. Confirmed hemodynamically significant malperfusion affected the spinal cord (2.7%), celiac (24%), superior mesenteric (40%), renal (51%), and iliofemoral (43%) arterial distributions. Of the 182 patients, 99 (54%) underwent aortic fenestration/stenting, 108 (59%) had 1 or multi-branch vessel fenestration/stenting, 5 (2.7%) had concomitant thoracic endovascular aortic repair, 17 (9.3%) had additional thrombolysis or thromboembolectomy, and 48 (26%) received no intervention. After fenestration/stenting, 24 patients (13%) required additional procedures for necrotic bowel or limb and 9 patients (4.9%) had subsequent aortic repair (thoracic endovascular aortic repair, open repair) before discharge. The new-onset paraplegia was 0%. The in-hospital mortality was 7.7% over 20+ years and 0% in the last 8 years. The 5- and 10-year survivals were 72% and 49%, respectively. The significant risk factors for late mortality were age and acute paralysis (hazard ratio, 3.5; both P < .0001). Given death as a competing factor, the 5- and 10-year cumulative incidence of reintervention was 21% and 31% for distal aortic pathology, respectively. CONCLUSIONS: Patients with acute type B aortic dissection with malperfusion can be managed with endovascular fenestration/stenting with excellent short- and long-term outcomes. This approach is particularly helpful to patients with static malperfusion of aortic branch vessels.

17.
J Agric Food Chem ; 67(48): 13282-13298, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31690068

RESUMO

Dietary supplementation with conjugated linoleic acid (CLA) has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates dextran sulfate sodium (DSS)-induced colitis in mice. CLA was administered in five different doses: 40, 20, 10, 5, and 2.5 mg/day. Doses of CLA at 10 mg/day and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40, 20, and 10 mg/day CLA significantly increased the concentration of mucin2 and goblet cells, but neither 5 mg/day CLA nor 2.5 mg/day CLA had any effects. Meanwhile, 40 and 20 mg/day CLA treatments significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative-stress-related enzymes (superoxide dismutase, glutathione peroxidase, and catalase) and inflammatory cytokines [tumor necrosis factor-α, interleukin (IL)-10, and IL-6] were modulated by 40 and 20 mg/day CLA. Furthermore, 40 mg/day CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter. In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier, and reverting microbiota changes.

18.
Int J Med Robot ; : e2042, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31702110

RESUMO

BACKGROUND: Haptic devices with active translation and orientation outputs are highly preferred in surgical teleoperation. However, commercial products are expensive, while state-of-the-art research prototypes are difficult to reproduce outside the original laboratories. METHODS: This paper presents the design and experimental characterizations of two styluses for the CombX, a haptic device with both force and torque outputs constructed from two TouchX haptic devices, which have only force outputs at their styluses. The arrangement was optimized to improve the specifications. Additional functions for surgical teleoperation were also integrated. RESULTS: The CombX has a translation workspace larger than 160 × 160 × 160 mm3 . After calibration, it can provide force outputs of up to 16.32 N and torque outputs of up to 316 mNm. The CombX has also been successfully used to teleoperate a continuum surgical manipulator for two surgical tasks. CONCLUSION: The results show that the CombX is a viable option for surgical teleoperation.

19.
Toxicol Lett ; 319: 102-110, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31706006

RESUMO

Crizotinib is a multi-target receptor tyrosine kinase inhibitor which is of great importance for the management of ALK-rearranged non-small cell lung cancer (NSCLC) patients. Serious erythroderma and toxic epidermal necrolysis have been reported associated with crizotinib treatment. The underlying mechanisms have not been examined. In this study, we tested the toxicity of crizotinib on immortal human keratinocytes (HaCaT) and human primary keratinocytes. We found that crizotinib directly cause cytotoxic on these two cells, which could be the explanation of the clinical characteristic of pathology. Apoptosis was observed and Z-VAD-FMK, a pan-caspase inhibitor can almost totally reverse the apoptosis induction effect of crizotinib. However, mitochondrial dysfunction and DNA damage were not involved in crizotinib-induced apoptosis, indicating the intrinsic apoptosis pathway have no connection with this cutaneous toxicity. Further studies showed that crizotinib significantly increased cleaved-caspase-8, a signaling protein of extrinsic apoptosis pathway, in a concentration and time-dependent manner. Moreover, we found the targets of crizotinib were not involved in HaCaT cells apoptosis. Collectively, our findings first report keratinocytes apoptosis is the key cause of crizotinib-induced cutaneous toxicity. We also reveal crizotinib induce apoptosis through the extrinsic apoptosis pathway due to detected up-regulated cleaved-caspase-8. Meanwhile, the apoptosis is independent of mitochondrial dysfunction, DNA damage and related drug targets inhibition.

20.
Stem Cell Res Ther ; 10(1): 352, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31779687

RESUMO

BACKGROUND: Traumatic brain injury (TBI) is a common neurotrauma leading to brain dysfunction and death. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold promise in the treatment of TBI. However, their efficacy is modest due to low survival and differentiation under the harsh microenvironment of the injured brain. MG53, a member of TRIM family protein, plays a vital role in cell and tissue damage repair. The present study aims to test whether MG53 preserves hUC-MSCs against oxidative stress and enhances stem cell survival and efficacy in TBI treatment. METHODS: In this study, we performed a series of in vitro and in vivo experiments in hUC-MSCs and mice to define the function of MG53 enhancing survival, neurogenesis, and therapeutic efficacy of stem cells in murine traumatic brain injury. RESULTS: We found that recombinant human MG53 (rhMG53) protein protected hUC-MSCs against H2O2-induced oxidative damage and stimulated hUC-MSC proliferation and migration. In a mouse model of contusion-induced TBI, intravenous administration of MG53 protein preserved the survival of transplanted hUC-MSCs, mitigated brain edema, reduced neurological deficits, and relieved anxiety and depressive-like behaviors. Co-treatment of MG53 and hUC-MSCs enhanced neurogenesis by reducing apoptosis and improving PI3K/Akt-GSK3ß signaling. CONCLUSION: MG53 enhances the efficacy of hUC-MSCs in the recovery of TBI, indicating that such adjunctive therapy may provide a novel strategy to lessen damage and optimize recovery for brain injury.

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